Absstract of: NL2031876A

A method for co—producing methane and bio—oil by coupling anaerobic digestion of domestic wastewater with microalgae cultivation is provided, including: (1) removing suspended matters from domestic wastewater; (2) performing anaerobic digestion fermentation. reaction; (3) purifying, desulfurizing, and drying marsh gas: one part of the marsh gas is used for combustion for power generation, and the other part is used for separating CO2 and biomethane; CO2 generated by the combustion for power generation enters an oil—enriched, microalgae cultivation system; the combustion for power generation supplies electric energy to a light source; (4) adding water into biogas slurry for dilution, performing sterilization treatment and aeration treatment, and adjusting a pH value; (5) applying the pretreated biogas slurry to oil—enriched microalgae cultivation; and (6) squeezing oil— enriched, microalgae to obtain bio—oil, and returning remaining algae residues to the anaerobic digestion fermentation reaction.

Absstract of: CA3134576A1

A system and method of using a biofilter for removal of heavy metals. A biofilter apparatus for extracting heavy metals from wastewater using Spirulina microalgae is disclosed. The biofilter apparatus comprises a column having a column cap and a base, an inlet hose connected to the column cap, an outlet hose connected to the base, a plurality of holder disks connected to the base, a microfilter found between the holder disks, and a plurality of microbeads contained in the column. The microbeads are coated with a microalgae wherein the microalgae purifies the wastewater that flows through the inlet hose and produces cleaner wastewater that exits through the outlet hose. The microfilter filters out heavy metal (e.g., mercury, cadmium, lead, copper, and zinc) from the wastewater to form biomass briquettes. A plurality of biofilter apparatus may be connected in series to create a biofilter system.

Absstract of: US2023110971A1

A system, comprising a Trickle Bed Reactor (TBR), a microalgae cultivation module, and a feedback module is used to sustainably boost CO2 fixation for growing micro-algae. The TBR comprises a packing material in the form of non-porous particles with a high surface-to-volume ratio, forming a substrate for attachment of Volatile Fatty Acid (VFA) producing microbes, fed with CO2 (and/or CO), H2, nutrients, and a moistening liquid. The TBR output is fed to the microalgae cultivation module which uses micro-algae selected or adapted for increased productivity in the presence of VFAs. No CO2 needs to be fed to the microalgae cultivation module. At least part of the output of the microalgae cultivation module is fed by the feedback module back to the TBR either as a source of nutrients or for as a means backflushing for unclogging or expulsing the packing material from the TBR for cleaning/disinfection. The overall CO2 balance of the system operation is negative.

Absstract of: US2023115516A1

Provided are a culture apparatus and a culture method with which microalgae can be cultured satisfactorily. The culture apparatus includes a plurality of culture tanks and a water storage tank, and cultures microalgae in a culture solution. Each of the plurality of culture tanks has a translucent accommodating portion containing the culture solution and microalgae. The volumes of the accommodating portions of the plurality of culture tanks are different from each other. The water storage tank has a translucent water storage unit for storing a stored water. The plurality of culture tanks are selectively arranged in the water storage unit.

Absstract of: US2023114259A1

The present invention can: efficiently and readily produce glucose from microalgae that accumulate starch in their cells; and obtain ethanol. During a preparation step of the glucose production method, microalgae are prepared on which a saccharifying enzyme acts on starch accumulated inside the microalgae cells, without disrupting the cell walls. In a saccharification step, starch inside the cells is saccharified and glucose is generated, by adding a saccharifying enzyme to the microalgae without a disruption treatment. The ethanol production method has a step in which, after the saccharification step, the glucose undergoes alcoholic fermentation and ethanol is generated.

Absstract of: WO2023051077A1

Provided are a genetically engineered strain for producing polylactic acid and a method for producing polylactic acid. The method comprises: synthesizing a D-lactate dehydrogenase gene, a propionyl-CoA transferase gene and a polyhydroxyalkanoate synthase gene into a target gene, and performing double enzymatic cleavage on same and a vector, purifying, and transforming a competent cell to obtain a recombinant plasmid; extracting DNA of the recombinant plasmid and introducing the DNA into an engineering strain, and extracting and verifying to obtain an engineering cell strain; and then culturing same in a culture medium, introducing carbon dioxide and light into the culture medium, centrifuging, drying, and recycling. According to the present invention, by modifying natural cyanobacteria and using a high density fermentation strategy, the biomass of the cyanobacteria is greatly improved. According to the present invention, the problem in the prior art that only a sugar-derived raw material can be used to produce polylactic acid is solved.

Absstract of: GB2595644A

Disclosed are modified strains of Chlorella vulgaris having a reduced chitin content. The reduced chitin content may improve digestibility. Also disclosed is a method for producing them. The method involves performing mutagenesis of a parent strain of Chlorella vulgaris. The mutation may be induced with a mutagen such as ethyl methanesulphonate. Also disclosed is a composition comprising algae biomass derived from the modified strains of Chlorella vulgaris and their use in food and/or cosmetics amongst other applications.

Absstract of: CN115885041A

The present technology relates to a method for producing transformed and fully isolated cyanobacteria, said method comprising incubating cyanobacteria and a nucleic acid under conditions suitable for transforming cyanobacteria with a nucleic acid comprising a selection marker; further incubating cyanobacteria in the growth medium under conditions suitable for cyanobacteria recovery; the transformed and fully isolated cyanobacteria are selected using a selective agent.

Absstract of: CN115873714A

The invention discloses a microbial agent for treating and recycling carbon, nitrogen and phosphorus in high-salinity wastewater and application thereof. The microbial inoculum comprises seawater microalgae and fungal hyphae. The microbial agent can efficiently treat carbon, nitrogen and phosphorus in high-salinity wastewater, operation is easy, and carbon, nitrogen and phosphorus in biomass recycled after treatment can be recycled. The problems that the efficiency of treating carbon, nitrogen and phosphorus in high-salinity water by a current microbiological method is not high, and high-salinity-resistant microalgae are difficult to recover are solved; a better effect can be achieved when the salinity of the wastewater is below 4%. In addition, PVA and sodium alginate do not need to be added to form gel balls and then added into the wastewater, appropriate algae and bacteria are directly added into the wastewater, the algae and the bacteria can form the balls in the wastewater while treating carbon, nitrogen and phosphorus in the wastewater, then the balls are fished and recycled together subsequently, and operation is simpler and more convenient.

Absstract of: CN115873685A

The invention provides a pneumatic stirring type photobioreactor. The pneumatic stirring type photobioreactor comprises a shell, a paddle wheel and an aeration pipe, the paddle wheel is rotatably mounted in the shell; the aeration pipe is mounted below the paddle wheel, the aeration pipe performs aeration towards one side of the paddle wheel so as to push the paddle wheel to rotate, and gas introduced into the aeration pipe at least contains carbon dioxide. The gas containing carbon dioxide provides a carbon source for photosynthesis of the microalgae and promotes growth of the microalgae; on the other hand, gas in the aeration pipe forms rising bubbles in the algae liquid, after the bubbles impact the paddle wheel, kinetic energy and pressure energy of the bubbles are greatly partially converted into mechanical energy of the paddle wheel, and the paddle wheel hinders the rising motion of the bubbles, so that the retention time of the bubbles in the algae liquid is prolonged; therefore, carbon dioxide gas in the bubbles is promoted to diffuse to surrounding algae liquid; the paddle wheel is driven by gas, so that the energy consumption and extra cost are reduced, and the economical efficiency is high; meanwhile, the flow condition of the algae liquid is improved through rotation of the paddle wheel, and microalgae cells can better receive illumination.

Absstract of: RO137350A2

The invention relates to a biotechnological process for obtaining Chlorella sorokiniana UTEX 1230 green microalgae biomass. According to the invention, the process consists in that the biomass of Chlorella sorokiniana UTEX 1230 microalgae strain cultivated in a medium known per se, in the exponential growth phase, having a optical density (OD) of about 0.05 OD units at 660 nm, is irradiated with γ rays of 75 Gy, at a dose flow rate of 0.1...1.0 Gy/s, for 1...10 min, until the algal culture reaches, by growth and cell multiplication, about 0.2 OD units at 660 nm, the so irradiated culture being admixed with a 150...230 times higher amount of non-irradiated culture medium and maintained for 7 days at atmospheric pressure, at a temperature of 30°C and illumination of 16500 lux, in a medium continuously bubbled with atmospheric air, to result in an increase of harvested biomass yield , of total proteins and lipids, of total carotenes and chlorophyll.

Absstract of: US2023101427A1

A system for producing microalgae in the form of biofilm, including a frame, a motor, at least one support and a web designed to receive the biofilm and in which the frame is configured to support the motor, the motor is configured to impart a rotational movement on at least one support, the at least one support is configured to have a rotational movement about an axis of rotation and to support and impart the rotational movement on the web, and the web is configured to at least partially surround the at least one support, wherein that the support is configured to have a non-negative buoyancy in a liquid.

Absstract of: WO2023047059A1

The present invention relates to a photobioreactor (3) for producing biomass in a closed system, comprising: - a biomass receptacle comprising a first flat wall (30) and a second flat wall (31), the two flat walls (30, 31) being parallel; - a base (32) extending perpendicularly from an edge (300) of the first flat wall (30) to an edge of the second flat wall (31), the two flat walls (30, 31) and the base (32) together defining a space for the growth of biomass; - movement means (5) which are mounted on the base (32) and are rigidly connected to the biomass receptacle so as to be translatable in a sliding direction (B) and which are configured to guide a translational movement of the biomass receptacle along a track (23) of a supporting structure (2) bearing the photobioreactor, parallel to the sliding direction (B).

Absstract of: KR20230042652A

본 발명은 광변동 조건하에서 높은 세포 성장률 및 지질 생산성을 갖는 돌연변이 미세조류에 관한 것이다.

Absstract of: CN218755740U

The utility model relates to a carbon sink microalgae culture assembly, and belongs to the technical field of microalgae culture biology. The carbon sink microalgae culture assembly comprises a transparent bottle body, and a liquid discharge pipe is arranged at the bottom of the bottle body; the transparent bottle cap covers the bottle opening of the transparent bottle body, the transparent bottle cap is provided with an exhaust port and a mounting hole, and the exhaust port is provided with a first filter membrane; the transparent hard tube is inserted into the mounting hole, the two ends of the transparent hard tube are a sealed end and an open end respectively, the sealed end and the open end are located inside and outside the transparent bottle body respectively, and the section, located in the transparent bottle body, of the transparent hard tube is a bent tube; the lamp strip is inserted into the bent pipe through the open end; the air outlet end of the air supply assembly is communicated with the interior of the transparent bottle body. Through the structure, the assembly not only can be used for culturing microalgae, but also can be used for purifying air, and the lamp strip in the bent pipe covers a wider area in the culture solution, so that the illumination intensity obtained by the microalgae in each area in the culture solution is more balanced, and the growth conditions of the microalgae in each area in the culture solution tend to be consistent.

Absstract of: CN218755771U

The utility model discloses a gas circulation absorption device for a microalgae culture photoreactor, which comprises a bottom plate, a shell and a gas pipeline are arranged on the bottom plate, the bottom plate is fixed on the bottom plate through screws, a vertical partition plate is arranged in the middle of the shell and divides the shell into a left part and a right part, a shell on the left side of the vertical partition plate is a gas mixing cabin, a CO2 sensor is arranged in the gas mixing cabin, a gas mixing pipeline connected with a CO2 gas cylinder is arranged on the left side of the gas mixing cabin, and a gas flow controller is arranged in the middle of the gas mixing pipeline. According to the microalgae carbon sequestration device, CO2 which is not absorbed by microalgae and generated oxygen can be collected, after the oxygen is filtered out, the CO2 concentration is configured again according to the set proportion, and the CO2 is recycled, so that the carbon sequestration efficiency of the microalgae is improved, the production cost is reduced, and the CO2 utilization rate and the economic benefit are improved.

Absstract of: CN218755718U

The utility model discloses a photoreactor for stage culture of microalgae, which comprises a culture cabin main body, a control module, a pipeline type culture cabin, a carbon dioxide gas cylinder, an algae seed liquid storage tank, a culture liquid tank and an acid-alkali liquid tank, and the top of the culture cabin main body is fixedly connected with the control module; the culture cabin main body is sequentially provided with a first-stage culture cabin, a second-stage culture cabin and a third-stage culture cabin from bottom to top, partition plates are arranged among the first-stage culture cabin, the second-stage culture cabin and the third-stage culture cabin, and the right side of the first-stage culture cabin is respectively connected with a carbon dioxide gas cylinder, an algae seed liquid storage tank, a culture liquid tank and an acid-alkali liquid tank through pipelines; the pipeline type culture cabin is sequentially provided with a liquid inlet valve, a liquid inlet pump machine, a liquid outlet pump machine and a liquid outlet valve in the liquid flowing direction. By adopting the novel structural design, the microalgae multi-stage culture which can be realized by a plurality of photoreactors originally is simplified to be realized by only one photoreactor, so that the production cost is greatly saved.

Absstract of: CN115849571A

The invention discloses a photovoltaic-driven baffle plate sludge-condensation microalgae reactor system, which is characterized in that sewage sequentially passes through an anaerobic reaction zone, a microalgae reaction zone and a membrane separation zone, and a photovoltaic power supply driving unit supplies energy to system operation. The anaerobic reaction zone comprises an anaerobic baffling reaction cell and a buffer cell, and is used for removing most organic matters and SS (suspended solids) in sewage; the microalgae reaction area is divided into different space units by baffles, an aeration device is mounted at the bottom of the reaction area, a light gathering device is arranged in each space unit to provide a light source for microalgae growth, finally, sewage enters the membrane separation area to be subjected to algae-water separation, and high-concentration microalgae further removes N and P which cannot be effectively removed in the anaerobic section. The method can be applied to treatment of rural domestic sewage with a low C/N value, and mainly solves the problems that N and P cannot be effectively removed in the anaerobic sewage treatment process and the system operation energy consumption is low.

Absstract of: CN115851446A

The invention discloses a method for blocking DHA (docosahexaenoic acid) synthesis in oil-producing microalgae and increasing the relative content of EPA (eicosapentaenoic acid), the DHA synthesis in the oil-producing microalgae is blocked and the relative content of EPA is increased by performing gene editing on a delta5 elongase gene for coding C20 fatty acid elongase, and meanwhile, an engineering algal strain which does not produce DHA and is enriched with EPA is obtained, and the nucleotide sequence of the engineering algal strain is shown as SEQID NO.3. The invention also discloses a method for blocking DHA synthesis in oil-producing microalgae and increasing the relative content of EPA. According to the invention, gene editing is carried out on oil-producing microalgae delta 5 elongase (Elo5), so that the metabolic pathway of EPA for further extension and desaturation synthesis of DHA is blocked, and the goal of accumulation of EPA with higher relative content is achieved at the same time.

Absstract of: KR20230041852A

본 발명에 따른 부상식 수처리장치는, 대칭적으로 이격 배치된 형상을 갖는 복수의 부상체; 상기 복수의 부상체의 내측 간을 연결하는 복수개의 고정용 막대; 및 상기 고정용 막대의 상단에 형성된 막대 고정홈 상에 분리 가능하게 결합하는 미세조류 고정틀;을 포함하고, 상기 미세조류 고정틀은 상기 고정용 막대의 상단에 형성된 막대 고정홈 상에 분리 가능하게 결합하는 고정틀 바아 및 상기 고정틀 바아의 하단 상에 결합하는 고정틀 망을 포함하며, 상기 고정틀 망 상에 복수의 미세조류 군집이 고정되는 것을 특징으로 한다.

Absstract of: CN218710374U

The utility model relates to the technical field of microalgae culture equipment, and discloses algae culture equipment which is characterized in that the upper end of a rotating shaft is fixedly connected with a rotating disc matched with the interior of a culture box in size, the upper end of the rotating disc is circumferentially and fixedly connected with fixed columns at equal intervals, the fixed columns are cylindrical, and the side surfaces of the fixed columns are provided with mounting grooves; the mounting groove is a rectangular groove, and a heating rod is arranged in the mounting groove. According to the algae culture device, algae needing to be cultured are placed in the culture box, the heating rod is started, the heating rod rapidly transmits the temperature to the clamping block, the clamping block rapidly transmits the temperature to culture liquid of the algae, the mounting frame pressurizing device is started, and the mounting frame pressurizing device drives the mounting frame oxygen generator to rotate, so that the algae culture device is started, and the algae culture device is started. A mounting frame oxygen generator drives a mounting frame connecting pipe to rotate, and the mounting frame connecting pipe drives a fixing column, a heating rod and a mounting groove to rotate, so that a culture solution is uniformly heated, and the heat conduction efficiency is improved.

Absstract of: CN218710393U

The utility model provides a photoreactor with a turbulent flow device, which comprises a reactor, the reactor is of a rectangular box body structure, a liquid inlet box is arranged on the top surface of the reactor, the inside of the liquid inlet box is communicated with the inside of the reactor, the top surface of the reactor is connected with an exhaust valve, the inside of the reactor is connected with a turbulent flow assembly, and the turbulent flow assembly is connected with the liquid inlet box. A plurality of groups of turbulent flow assemblies are arranged in parallel, an aeration disc is arranged on the bottom surface in the reactor, the bottom of the aeration disc penetrates through the reactor through a guide pipe to be connected with a CO2 gas cylinder, and the aeration disc is used for introducing CO2 gas into the reactor. According to the utility model, the plurality of groups of turbulent flow assemblies are arranged in the reactor, so that when CO2 gas is introduced into the reactor from bottom to top by the aeration disc, the turbulent flow assemblies are utilized to block and slow down the floating speed of bubbles formed by the CO2 gas in the reactor, thereby prolonging the retention time of the CO2 gas in algae liquid, enabling microalgae to fully absorb CO2, and further improving the microalgae quality. The growth efficiency of the microalgae is improved.

Absstract of: WO2023043063A1

The present application relates to a novel microalgae strain with the efficacy of promoting plant growth and a use thereof. When cultured in the presence of fermentation exhaust gas, a novel Chlorella vulgaris CD02-3002 strain according to an aspect grows fast with a high efficiency of photosynthesis and thus can effectively reduce the exhaust gas generated by microbial fermentation. Having the effect of promoting plant growth when applied to plants, a culture of the strain or a supernatant of the culture can be used as a fertilizer for plants and thus can be advantageously applied as a novel carbon reduction technique.

Absstract of: WO2023040130A1

A pharmaceutical composition for radiation protection, and a preparation method therefor and the use thereof. Amifostine and natural microalgae in the pharmaceutical composition are in osmotic pressure driving combination, and are thus gradually degraded in the intestinal tract after oral administration, fully cover the intestinal tract, and are slowly released. The drug concentration in small intestine tissues is significantly increased, and the radiation protection effect on the intestinal tissues is fully developed; meanwhile, the systemic toxic and side effects of the drug are avoided. The pharmaceutical composition can also achieve the effects of nutrient supplement and intestinal inflammation regulation, has a good oral safety and a simple and feasible preparation process, is easy to store and take, solves the problems of a low absorption efficiency and oral safety of a drug and a preparation thereof in the prior art, and has wide application prospects in the field of intestinal radiation protection.

Absstract of: WO2023043298A1

The present invention relates to mutant microalgae with a high cell growth rate and high lipid productivity under fluctuating light conditions. The Chlamydomonas reinhardtii mutant strain provided by the present invention has a high cell growth rate and high lipid productivity under fluctuating light conditions, and thus can be used in various applications, for example, the analysis of physiological responses and metabolic pathways of cells associated with various light conditions.