The present invention is directed generally to chimeric proteins that can facilitate targeting of nanoparticle carriers to antigen presenting cells, and to nanoparticulate carriers comprising these chimeric proteins. An embodiment of the chimeric protein comprises of a TLR5 agonist such as a flagellin, and a gpl20-derived peptide. The invention is also directed to methods of internalising an antigen in an antigen presenting cell, and methods of eliciting an immune response to an antigen in a subject, using the nanoparticulate carriers comprising the chimeric proteins.

Antibody molecule-drag conjugates (ADCs) that specifically bind to lipopolysaceharides (LPS) are disclosed. The antibody molecule-drug conjugates can be used to treat, prevent, and/or diagnose bacterial infections and related disorders.

The subject relates to an isolated O25b antigen of multi drug resistant (MDR) E. coli strains and immunogens or vaccines containing the same.

Antibody molecule-drug conjugates (ADCs) that specifically bind to lipopolysaccharides (LPS) are disclosed. The antibody molecule-drug conjugates can be used to treat, prevent, and/or diagnose bacterial infections and related disorders.

The present invention relates to a novel bacteriophage having a specific bactericidal activity against salmonella, a composition for the prevention or treatment of infectious diseases comprising the bacteriophage as an active ingredient, an antibiotic comprising the bacteriophage as an active ingredient, an animal feed or drinking water comprising the bacteriophage as an active ingredient, and a sanitizer or cleaner comprising the bacteriophage as an active ingredient. The novel bacteriophage of the present invention has a specific bactericidal activity against Salmonella choleraesuis, Salmonella typhimurium, Salmonella derby, Salmonella infantis or Salmonella newport with no influences on beneficial bacteria, as well as excellent acid- and heat-resistance and desiccation tolerance. Therefore, the novel bacteriophage can be used for the prevention or treatment of salmonellosis or salmonella food poisoning, which is an infectious disease caused by Salmonella choleraesuis, Salmonella typhimurium, Salmonella derby, Salmonella infantis or Salmonella newport, and also widely used in animal feeds, drinking water for livestock, sanitizers, and cleaners.

The present invention relates to method of treating and/or preventing inflammation in a subject caused by bacteria that express HtrA. The present invention further relates to immunogenic compositions comprising an HtrA polypeptide or fragment thereof, as well as a therapeutic and/or prophylactic vaccine for treating or preventing disease.

It was discovered that when a combination consisting of a protein secreted by a bacterium belonging to the genus Salmonella with dead cells of a bacterium belonging to the genus Salmonella was administered to a living organism, a highly excellent defense effect against the infection with a bacterium belonging to the genus Salmonella was achieved compared with the case of administering either the protein or the dead cells singly. Thus, provided is a vaccine that has a high safety and an excellent defense capability against salmonellosis.

Provided herein are methods of producing a heterologous polypeptide and compositions comprising same.

In one aspect, the present disclosure provides compounds of formulae I and II. In another aspect, a compound of formula I or II is formulated into compositions with an antigen, optionally with a vesicle. In some embodiments, compositions are administered intramuscularly.

Detection of microbial pathogens related to bacterial infections through amplification especially by RT- LAMP The invention is in the field of the detection of microbial pathogens related to bacterial infections. More particularly, the invention relates to methods and products, particularly primers, for the simultaneous or individual detection of Salmonella spp., Staphylococcus aureus, Streptococcus pneumoniae, Haemophilus influenzae and/or Escherichia coli, in particular for performing this detection using isothermal amplification of specific genes of these pathogens, and more particularly using loop-mediated isothermal amplification (LAMP), either as end-point assay or as real-time LAMP assays.

种猪的养殖方法，包括以下步骤：(1)整理猪圈；(2)养殖：将断奶后的仔猪移到猪圈中饲养，每天喂食3～4顿，每头猪每天喂食1.5～3.5kg猪饲料；所述猪饲料包括以下按重量份计的组成成分，玉米60～80份、苜蓿20～40份、洋槐树叶20～30份、鱼骨7～9份、蕨菜5～9份、藕6～8份、韭菜4～6份、黄瓜4～6份、莴苣4～6份、桂圆6～8份、松子仁6～8份、番桃5～7份、凤梨5～7份、金桔3～5份、酸菜10～15份、葡萄酒3～5份；(3)放养；(4)病症防治；(5)出圈。本发明的猪的养殖方法，以种健康的方式加快猪的生长速度。

本发明提供种弱毒肠炎沙门氏菌疫苗，其中的抗原是保藏编号为CGMCC NO.13251的重组肠炎沙门氏菌疫苗株。本发明提供的疫苗安全可靠，不会造成散毒危险；常规注射疫苗会产生应激，疫苗吸收效果不好，而本疫苗可以通过口服免疫，并且避免免疫原在到达肠粘膜之前被降解的可能；本疫苗集载体与佐剂为体，不但能诱发机体产生体液免疫，而且能产生细胞免疫和粘膜免疫，既可以防控IBDV的侵害，又可以在定程度上防控沙门氏菌的感染；本疫苗生产工艺简单方便，生产成本极低，不需提纯蛋白，只需将疫苗菌扩大培养，冻干即可，口服免疫，节省人力成本。

Disclosed is a method for producing partially purified extracellular metabolite preparation from the probiotic bacterial strain Bacillus coagulans SBC-37 (Deposited in the Microbial Type Culture Collection and Gene Bank as strain number MTCC 5856) exhibiting 99% genetic homology with the known bacterial strains Bacillus coagulans ATCC 31284, Bacillus coagulans NBRC 3887 and Bacillus coagulans ATCC 7050. Also disclosed is the anti-microbial profile of said extracellular metabolite preparation against a panel of microbial pathogens, including synergistic anti-microbial effects of preparation when combined with a synergistic preservative blend comprising from about 61% w/w of thymol, about 38% of monolaurin and about 1% w/w of magnolol obtained from supercritical fluid extracts of Magnolia officinalis. The extracellular metabolite preparation alone or the combination of said extracellular metabolite preparation and preservative blend also inhibits microbial biofilm formation in a synergistic manner.

The present invention relates to method of treating and/or preventing inflammation in a subject caused by bacteria that express HtrA. The present invention further relates to immunogenic compositions comprising an HtrA polypeptide or fragment thereof, as well as a therapeutic and/or prophylactic vaccine for treating or preventing disease.

The present invention relates to vaccines comprising an effective amount of at least one antigen in a water-in-oil-in-water (WOW) emulsion and an additional adjuvant, and to the methods of preparation and uses thereof.

The invention relates to bird feed comprising synthetic capsaicinoids for prohylactic use or treatment of salmonella infection.

Provided are methods of treating diarrhea, particularly malabsorption diarrhea, in neonatal, young and adult non-human animals, in which the diarrhea results from infection of the animals by Salmonella spp., such as Salmonella typhimurium, with a therapeutically effective amount of a proanthocyanidin polymer from Croton lechleri, in either enteric or non-enteric form.

Provided herein are (1) a method of mixing an aluminum salt-adsorbed immunogen with a monophosphoryl lipid A (MPLA)-containing liposome (L(MPLA)), and (2) the resulting immunogenic composition. The resulting immunogenic composition has an enhanced immunostimulation potency compared with either a composition comprising the uncapsulated immunogen mixed with the L(MPLA) or the aluminum salt-adsorbed immunogen alone.

A method for increasing the presentation of ETEC CS6 antigen on cell surface, comprising the step of contacting cells expressing said antigen with an aqueous solution comprising 0.6-2.2 percent phenol by weight, such that the presentation of said antigen is increased by at least 100 %. A method for the manufacture of a killed whole cell vaccine for immunization against CS6-expressing ETEC. Cells and vaccines obtainable by the above methods.

A device for detecting an analyte, within a sample, comprising a housing member. The housing member comprising an inlet, a force member (2070), a moveable locking member (2065), an analyte detection membrane system (2047), an attachment member (2066), and a channel system (2300). The inlet allows a sample to be added into the system for testing and connects with the channel system (2300). The force member (2070) provides a compressing force to the analyte detection membrane system (2047). The moveable locking member (2065) maintains the force member against the membrane system until the sample is ready for testing. The locking system may be activated to release the force member and remove the compressing force from the membrane system. The analyte detection membrane system (2047) comprises a conjugate pad, a permeable membrane, a test membrane and an absorbent member. The membrane system may be arranged with each layer stacked one on top of the other such that the fluid from the channel system may flow through each layer in order. The attachment member (2066) may be attached to the moveable locking member (2065) and the conjugate pad (2050) such that the conjugate pad may be removed from the membrane system when the locking member is actuated. Removal of the conjugate pad can expose the test membrane underneath. The device may comprise multiple membrane systems each arranged to test for a different analyte, the channel system may comprise multiple channels for directing a por

Disclosed is an a whole killed bacterial cell anti-inflammatory composition in the manufacture of a medicament for treating an individual for an inflammatory disease of the bone, wherein the anti-inflammatory composition comprises a whole killed bacterial cells selected or formulated so that together they are specific for at least one microbial pathogen that is pathogenic in the bone, and wherein the microbial pathogen is selected from the group consisting of Staphylococcus aureus, coagulase-negative staphylococci, Streptococcus pyogenes, Streptococcus pneumoniae, Streptococcus agalactiae, other streptococci spp., Escherichia coli, Pseudomonas spp., Enterobacter spp., Proteus spp., Serratia spp. paNovirus B 19, rubella virus, hepatitis B.

The subject relates to an isolated antibody that specifically binds to O25b antigen of multi drug resistant (MDR) E. coli strains, its medical and diagnostic use, method of producing the antibody, including an isolated nucleotide sequence, plasmids and host cells as used in the production of the antibody; and further an isolated epitope recognized the specific antibody.

A carrier for detecting food poisoning bacteria that is used to simultaneously detect Escherichia coli, Salmonella, Staphylococcus aureus, and Vibrio parahaemolyticus includes a plurality of probes comprising: a probe from a region of pyrH gene of Escherichia coli; a probe from a region of vtx1 gene of Escherichia coli; a probe from a region of vtx2 gene of Escherichia coli; a probe from a region of invA gene of Salmonella; a probe from a region of dnaJ gene of Staphylococcus aureus; a probe from a region of toxR gene of Vibrio parahaemolyticus; a probe from a region of tdh gene of Vibrio parahaemolyticus; a probe from a region of trh1 gene of Vibrio parahaemolyticus; and a probe from a region of trh2 gene of Vibrio parahaemolyticus, wherein the probes are immobilized on the carrier.

The present invention provides compositions including siderophore receptor polypeptides and porins from gram negative microbes, and preferably, lipopolysaccarhide at a concentration of no greater than about 10.0 endotoxin units per milliliter. The present invention also provides methods of making and methods of using such compositions.

Vaccine vectors capable of eliciting an immune response to enteric bacteria and methods of using the same are provided. The vaccine vectors include a polynucleotide encoding a PAL polypeptide. The PAL polypeptide may be expressed on the surface of the vaccine vector. The vaccine vector may also include a second polypeptide encoding an immunostimulatory polypeptide such as a CD 154 polypeptide or an HMGB1 polypeptide.