本发明涉及种蛋白自组装新型纳米疫苗及其制备方法，基于抗原蛋白自组装制备而成，在疫苗制备过程中选择性引入分子佐剂，抗原含量≥85％，无需铝佐剂、弗氏佐剂等辅助即可引发高效免疫效应，利用物理调控暴露蛋白分子间的巯基，通过巯基/二硫键交换反应，形成以二硫键交联为主的稳定的蛋白纳米粒。本发明克服了传统纳米疫苗需引入外源载体或交联剂等缺陷，可同时提高疫苗的免疫效应及生物安全性。所得到的疫苗颗粒形貌规整，稳定性强，调控方式灵活，重复性好，可高效刺激树突细胞成熟。具有较强的通用性、普适性，在系列的抗原蛋白上得到验证，在疫苗新方法及生物制药领域具有潜在重大应用价值。

Intact, bacterially-derived minicells can safely introduce therapeutically effective amounts of plasmid-free functional nucleic acid to target mammalian cells. To this end, functional nucleic acid can be packaged into intact minicells directly, without resort to expression constructs, the expression machinery of the host cell, harsh chemicals or electroporation.

The present invention relates to a therapy for treating or preventing several diseases in animals, based on the administration of a highly concentrated avian derived immunoglobulins formulation, obtained from the egg yolk from hens previously hiper-immunized with a vaccine formulation comprising infectious agents or toxins antigens, a light mineral oil and a particulate adjuvant.

The present invention relates to a vaccine composition for prevention of salmonella infection, and a mixture adjuvant (hereinafter "BeesonBiologics adjuvant, BBL-A1") comprising a trivalent antigen mixture including an msbB gene-deleted Salmonella Galinarum, an msbB gene-deleted Salmonella Enteritidis, and an msbB gene-deleted Salmonella Typhimurium provides an inactivated trivalent vaccine composition (hereinafter "Trivac") for prevention of salmonella infection. Due to the redeeming side effects of conventional oily vaccine compositions in addition to simultaneously preventing the infection of the three serovars, the pharmaceutical composition of the present invention is effectively available for the prevention of salmonella infection.

The invention relates to a live vaccine for protection against enteric bacterial infection.

The present invention relates to the use of a composition selected from the group consisting of antibodies, antibody fragments, insulin-like growth factor antagonists, Toll-like receptor antagonists and mixtures thereof for the treatment or the prophylaxis of certain diseases.

The invention relates to a process for preparing an immunologic product based on chicken immunoglobulins. According to the invention, the process comprises the immunization of usual egg-laying hens with an antigen mass consisting of a mixture of bacteria, viruses and mushrooms, previously inactivated, in association with a usual adjuvant, after which immune response assessment tests are performed, the hens exhibiting a positive response being admitted for the egg production, wherefrom the eggs containing 100...200 mg lgY and 10...20 mg specific lgY, respectively, are selected and processed for preservation purposes.

The invention provides methods of formulating an anti-inflammatory composition for treating inflammatory conditions in a specific organ or tissue. The method involves selecting at least one pathogen that is pathogenic in the specific organ or tissue; producing an antigenic composition comprising antigenic determinants that together are specific for the pathogen; and formulating the antigenic composition for administration as an anti-inflammatory composition capable of eliciting an anti-inflammatory response in the specific organ or tissue, wherein the condition characterized by inflammation is not a cancer. In embodiments of the invention, a cancer is situated in the specific organ or tissue.

A method of treating or preventing a bacterial infection in an animal comprises administering to the animal an effective amount of an antibacterial peptide, the antibacterial peptide being derived from degraded date pits which are degraded by solid state degradation by a fungus, Trichoderma reesei. The antibacterial peptide has a molecular mass of less than 10 kDa and an amino acid sequence including (a) SEQ ID NO:4 or (b) SEQ ID NO:6. The bacterial infection is caused by a Gram-positive bacteria or a Gram-negative bacteria, for example, a Salmonella species, a Campylobacter species, a Shigella species, an Escherichia species, a Pseudomonas species, and a Staphylococcus species.

The present invention relates to compositions comprising improved flagellin derived constructs and methods of using the same in the treatment of various diseases.

A method of detecting a Salmonella microorganism is provided. The method includes the use of a selective growth medium, a first indicator system that is converted to a first detectable product by a Salmonella microorganism, and a second indicator system that is converted to a second detectable product by β-galactosidase enzyme activity. The method further comprises inoculating the growth medium and incubating the inoculated growth medium at a temperature higher than 40 degrees C.

A system and method for analyzing a biomarker in a biological sample is provided. A biological sample is loaded onto a microchip and an enzyme-linked immunosorbent assay specific to the biomarker is performed on the microchip. A color image of the microchip is generated using a mobile device and a color intensity of a selected portion of the color image is determined. The color intensity is correlated with a biomarker concentration using a baseline curve calculation and the concentration of the biomarker is then reported.

The present invention refers to new glycoconjugate antigens expressing built-in multiple epitopes and to polyvalent glycoconjugate vaccines intended for the protection of mammalians, and particularly for the protection of the human population from enteropathogenic bacteria, such as the Gram-positive anaerobic bacterium Clostridium difficile and the Gram-negative bacteria Salmonella typhi, Escherichia Coli, Vibrio Cholerae, Shigella flexneri, Salmonella typhimurium, Salmonella enteritidis, Salmonella paratyphi A, Shigella sonnei, Shigella dysenteriae, Salmonella cholerasuis, Klebsiella, Enterobacter,Pseudomonas aeruginosa and/or from viral gastrointestinal infections due to human noroviruses.

This invention relates to immunogenic compositions, particularly vaccine compositions, for use in providing protection against illness caused by bacterial infection with Shigella strains.

本发明公开了种融合表达鸡传染性法氏囊病毒VP2蛋白和沙门菌属外膜蛋白的重组乳酸菌菌株及其用途。本发明所述的重组乳酸菌菌株，含有经优化后的编码鸡传染性法氏囊病毒VP2蛋白的基因序列以及编码沙门菌属外膜蛋白OMP的基因序列。实验证明该重组乳酸菌菌株其能够高效融合表达VP2蛋白和外膜蛋白OMP，且以可溶性形式存在于细胞质中。将本发明的重组乳酸菌菌株直接作为疫苗免疫鸡，实验结果表明：该重组乳酸菌菌株能有效诱导机体产生特异性的免疫应答，主要产生特异性中和抗体，使免疫鸡获得100％的抵抗高致病力vvIBDV致死性攻击的保护，并可清除体内残留的病毒，达到清除性免疫的目的。因此，本发明的提出为鸡传染性法氏囊病的防治提供了种新的技术手段。

The invention discloses a synergistic composition comprising a mix of bacteria of the genera Lactobacillus and Propionibacterium which is particularly useful to reduce or eliminate contamination by bacteria of the genus Salmonella and fungi, thus also preventing the occurrence of mycotoxins in soybean meal and its derivatives.

There is a process which can pasteurize in-shell chicken eggs to inactivate pathogens when present which includes all strains of salmonella and all strains of viruses that historically have been known to exist within chicken eggs and currently are known to be evolving into new and separate strains which may cause large quantities of human illnesses unless countermeasures are developed and employed. One such countermeasure is provided through pasteurization of the subject in-shell eggs through pasteurization involving concurrently a secured environment together with a protocol which enables total inactivation of the targeted pathogens whether bacterial or viral without risk of recontamination.

This invention relates, in part, to methods and compositions that are useful for the treatment and/or prevention of various disorders, including radiation-related disorders, such as acute radiation syndrome.

The present invention relates to avirulent Salmonella Gallinarum variants by inactivating virulence gene clusters of Salmonella Gallinarum (SG), a main pathogen of avian salmonellosis, and various uses thereof notably in the production of Salmonella-specific lytic bacteriophages, pharmaceutical compositions and feed additives.

Provided herein is a field-effect transistor based sensor for real-time detection of water contaminants and methods of use thereof.

The present invention relates to compositions comprising improved flagellin derived constructs and methods of using the same in the treatment of various diseases.

The present invention relates to a therapy for the treatment or prevention of diverse diseases in animals, based on the administration of a formulation of highly concentrated immunoglobulins of avian origin obtained from egg yolk from hens previously hyperimmunised with a vaccine formulation comprising antigens of infectious agents or toxins, a light mineral oil and a particulate adjuvant.

The present invention relates to vaccines comprising an effective amount of at least one antigen in a water-in-oil-in-water (WOW) emulsion and an additional adjuvant, and to the methods of preparation and uses thereof.

The present disclosure relates to camel id antibodies that inhibit growth, and colonization of Salmonella serovars. The present disclosure also relates to a modified Lactobacillus as a delivery vehicle for controlling salmonella in a host organism.

Provided herein is a field-effect transistor based sensor for real-time detection of water contaminants and methods of use thereof.