Resumen de: WO2021195712A1

The technology relates to a method for producing a transformed and fully-segregated cyanobacteria, the method comprising incubating the cyanobacteria and a nucleic acid comprising a selectable marker under conditions suitable for transformation of the cyanobacteria with the nucleic acid; further incubating the cyanobacteria in growth media under conditions suitable for recovery of the cyanobacteria; and selecting the transformed and fully-segregated cyanobacteria using a selection agent.

Resumen de: EP4130273A1

The present invention relates to recombinant microalgal cells and their use in heterologous protein production, methods of production of heterologous polypeptides in microalgal extracellular bodies, microalgal extracellular bodies comprising heterologous polypeptides, and compositions comprising the same.

Resumen de: CN115677054A

The invention discloses a bacteria-algae photobioreactor which comprises an adjusting tank, a bacteria-algae mixing reactor and a microalgae recycling tank, the regulating tank is connected with a domestic sewage source and is used for collecting domestic sewage and regulating physicochemical properties of the domestic sewage to be suitable for growth of bacteria and algae; the bacteria-algae mixing reactor comprises a transparent columnar reaction container; the adjusting tank is connected with the reaction container through a first pipeline; and a first lifting pump and a first stop valve are mounted on the first pipeline. According to the bacteria-algae photobioreactor, microalgae does not need to be solidified, the microalgae can be directly inoculated into domestic sewage, the contact area is large, and the treatment efficiency is high. The method comprises the following steps: treating organic matters, N and P in sewage through bacteria and algae; according to the invention, the sewage is used as a raw material, then the microalgae is recycled through the microalgae recycling box through electric flocculation, the sewage is further treated in the microalgae recycling process through electric flocculation, suspended particles can be settled by destroying or neutralizing the repulsive force of particles suspended in water in the sewage, and some heavy metal ions can also be removed.

Resumen de: WO2023008787A1

Disclosed herein are a microalga-derived exosome mimetic and a manufacturing method therefor. The method for manufacturing an exosome mimetic of the present invention comprises a step of exclusion processing a buffer solution containing microalgae to prepare nanovesicles. The present invention has the advantage of being able to mass-produce exosome mimetics derived from microalgae on the basis of an extrusion processing method. In particular, the present invention can increase the yield of vesicle production of exosome mimetics derived from microalgae by selectively applying a biolipid surfactant that enhances extrusion processing efficiency.

Resumen de: WO2023008787A1

Disclosed herein are a microalga-derived exosome mimetic and a manufacturing method therefor. The method for manufacturing an exosome mimetic of the present invention comprises a step of exclusion processing a buffer solution containing microalgae to prepare nanovesicles. The present invention has the advantage of being able to mass-produce exosome mimetics derived from microalgae on the basis of an extrusion processing method. In particular, the present invention can increase the yield of vesicle production of exosome mimetics derived from microalgae by selectively applying a biolipid surfactant that enhances extrusion processing efficiency.

Resumen de: WO2023006827A1

The invention relates to a method of making a product for human consumption which comprises partially lysed microalgae, said method comprising a) preparing a suspension of microalgae, wherein the microalgae belong to a phylaselected from Chlorophyta, Ochrophyta, and Heterokonta; b) applying a pulsed electric field to the suspension of microalgae, wherein said pulsed electric field has a specific energy input between 25 to 150 kJ per kg suspension (kg sus-1); c) optionally treating the microalgae with enzyme; d) incubating the biomass suspension at 300 rpm, 4-37 °C for 6-48 h e) forming a biomass of partially lysed microalgae; and f) adding the biomass of partially lysed microalgae to a product for human consumption.

Resumen de: RO137238A2

The invention relates to a process for treating residual flows in dairy industry. According to the invention, the process consists in cultivating the Nannochloris sp. microalgae strain in a mixotrophic regime, by using 2.5...10 g/L lactose from whey obtained as a secondary flow during cheese-making, as a source of organic carbon and bubbling of air enriched with 7% CO2 as a source of inorganic carbon, in a photobioreactor equipped with an adjustable lighting source, thermostating and gas bubbling system, in alternating cycles of 12 h of natural/artificial lighting and 12 h of dark, at a temperature of 28 +/-1°C, with the addition of a whey volume corresponding to a concentration of 2.5...10g/L lactose, related to the total volume of nutrient medium, after 7 days of cultivation there resulting a 99...100% reduction of lactose content, up to 96% reduction of chemical oxygen consumption, up to 91% reduction of nitrogen content and 70% reduction of phosphorus content, respectively, and an increase of microalgae biomass quantity of up to 138%.

Resumen de: WO2023001894A1

Provided are compositions containing extracellular vesicles from microalgae (MEVs) that are exogenously loaded with bioactive cargo or other cargo of interest, such as for therapeutic, industrial, diagnostic, and cosmetic uses. The MEVs have a variety of applications as therapeutics, including as vaccines, as anti-cancer therapeutics, as diagnostics, and other such uses.

Resumen de: US2023022786A1

A food composition in the form of an oil-in-water emulsion, comprising 66 to 87 wt % of vegetable oil, less than 1 wt % of microalgal oil, water, microalgal protein, and wherein the composition has a pH of from 2 to 4.8, and wherein the composition is free from egg-derived ingredients.

Resumen de: WO2023001967A1

The present invention relates to a method of preparation of comprising microalgae, in particular Chlorella and products resulting thereof.

Resumen de: US2023025620A1

Extracellular vesicles derived from native, photosynthetic, non-fermenting microalgae are provided. A method for isolating extracellular vesicles from native, photosynthetic, non-fermenting microalgae involving growth, centrifugation and ultracentrifugation steps is also provided. Use of the isolated extracellular vesicles as carriers for deliverling diagnostic, therapeutic, nutraceutic and/or cosmetic agents is further provided.

Resumen de: EP4122330A1

The present invention relates to a method of preparation of comprising microalgae, in particular Chlorella and products resulting thereof.

Resumen de: CN115636864A

The invention provides a method for extracting protein by wall breaking of microalgae, which comprises the following steps: screening a target algae raw material, preparing the raw material subjected to wall breaking treatment, and adjusting the moisture content in the target algae raw material to prepare a microalgae sample; after the temperature of the to-be-frozen area and the temperature of the melting area are adjusted to set values through a refrigerating machine, the microalgae samples are placed on a rotating conveying belt, so that the microalgae samples are rapidly frozen in the freezing link and then enter a rapid melting area along with operation of the conveying belt; the molten microalgae sample is conveyed to an extraction section for protein extraction, a refrigerant in a refrigerator absorbs heat after expansion, and a low-temperature environment is provided for the freezing area; and repeating the steps to form a complete protein extraction process. According to the invention, the frozen algae are quickly melted by using heat generated in the refrigeration process of the refrigerator, so that the energy is saved, the production of the freezing and quick melting section process is coherent, the process is simplified, and the efficiency is improved.

Resumen de: CN115637227A

The invention discloses an animal cell culture reactor system with zero carbon emission and application thereof. The animal cell culture reactor system comprises an animal cell culture reactor and a microalgae culture reactor, a gas inlet of the microalgae culture reactor is communicated with a gas outlet of the animal cell culture reactor, and a gas outlet of the microalgae culture reactor is communicated with a gas inlet of the animal cell culture reactor. The microalgae culture reactor is used for absorbing and treating carbon dioxide in waste gas produced in the animal cell culture process, and oxygen produced in the microalgae culture process is supplied to the animal cell culture system, so that green and environment-friendly treatment of carbon dioxide is achieved, and resources such as oxygen are saved. The method realizes energy conservation and emission reduction and cyclic utilization of waste resources, is a low-carbon and green animal cell culture technology, and fully responds to the requirements of carbon emission reduction and carbon neutralization.

Resumen de: CN115627241A

The invention discloses a culture method of nitrogen-fixing anabaena, which comprises the following steps: preparation of a culture medium and illumination culture: separately sterilizing leucine and arginine at high temperature, and adding the sterilized leucine and arginine into the sterilized basic culture medium; the zeatin is also added into the sterilized basic culture medium after being subjected to membrane filtration sterilization, an anabaena azotica seed solution is inoculated, illumination shaking culture is conducted for 7-9 days, and then the microalgae culture solution is obtained, and the microalgae cell density is 3.12 * 107-5.35 * 107 pieces/mL. According to the method, the anabaena azotica can grow rapidly, the number of the microalgae cells is increased, the culture time is shortened, and the production cost is reduced; the nitrogen-fixing anabaena culture solution can be used as a microbial fertilizer.

Resumen de: CN115627236A

The invention discloses a normal-temperature preservation method of a microalgae culture solution, relates to microalgae types including chlorella pyrenoidosa, anabaena nitrogen-fixing and micromonofidus, and belongs to the technical field of microalgae biology. The method comprises the following steps: adding triiodobenzoic acid and potassium sorbate into a microalgae culture solution (the concentration of microalgae cells is 2.0 * 10 < 7 >-4.0 * 10 < 7 >/mL), adding glycerol and sorbitol, adding nutrient substances (glucose and yeast extract powder), adjusting the pH value, and packaging by adopting an opaque plastic barrel. The microalgae culture solution is stored in a dry and shady room at normal temperature for 6 months, the concentration of living algae cells is 1.40 * 10 < 7 >/mL to 2.92 * 10 < 7 >/mL, the survival rate of the algae cells is 70%-73%, and the quality requirement of the microalgae culture solution as a microbial fertilizer is met.

Resumen de: CN115627237A

The invention belongs to the technical field of bioengineering, and discloses a method for increasing the yield of astaxanthin in haematococcus pluvialis by coupling proline with multiple stresses, which comprises the following steps: firstly, culturing haematococcus pluvialis in a growing period by adopting an abiotic stress coupling proline induction condition to enable algae cells to grow and accumulate biomass; and then culturing the microalgae by utilizing abiotic stress coupled proline in an induction period. Under an abiotic stress condition, 100 [mu] mol/L of proline is added, so that the astaxanthin content and the astaxanthin yield of the microalgae can be remarkably improved, rapid accumulation of the astaxanthin in algae cells is realized through the culture method, and a new research direction is provided for efficiently culturing haematococcus pluvialis to produce the astaxanthin. According to the method disclosed by the invention, the content of astaxanthin in haematococcus pluvialis is remarkably increased, and when the concentration of added proline is 100 mu mol/L, the content and yield of the astaxanthin in microalgae are 42.77 mg/g and 3.27 mg/L/d respectively, which are increased by 61.40% and 55.45% compared with those of a control group.

Resumen de: CN115627216A

The invention relates to the technical field of air supply and stirring of closed microalgae cultivators, and discloses an automatic stirring device of a closed microalgae cultivator, which comprises a driver and a stirring shaft driven by the driver, the driver is fixed on the closed microalgae cultivator, the stirring shaft is located in the closed microalgae cultivator, the driver is provided with a first air inlet hole, and the stirring shaft is provided with a second air inlet hole. The stirring shaft is provided with a first air outlet hole, and gas enters the driver from the first air inlet hole, is discharged from the first air outlet hole and then enters the closed microalgae culture device for microalgae to use. The closed microalgae culture device has the beneficial effects that the stirring shaft is driven to rotate by utilizing air flow for supplying air to the closed microalgae culture device, the air flow is sprayed out from the stirring shaft in a scattering manner and forms a vortex-shaped stirring effect along with the rotation of the stirring shaft, and the aim of stirring is fulfilled while air is supplied, so that the air can be fully dispersed into a microalgae culture solution; meanwhile, discharge of oxygen in the culture solution is facilitated, gas exchange of the culture solution is accelerated, and gas and nutrients are fully dispersed.

Resumen de: CN115624134A

The invention relates to the field of food, and particularly discloses a functional microalgae nutrition fortified fermented wheaten food, and steamed twisted rolls are prepared from the following raw materials: flour, edible microalgae powder, a sweet tea extract, milk powder, yeast, baking powder, edible soda ash and water. The prepared steamed twisted rolls are comprehensive in nutrition, one or more microalgae of chlorella pyrenoidosa, haematococcus pluvialis, euglena gracilis, micrococcus and chlamydomonas reinhardtii are combined, matched and mutually promoted, so that the steamed twisted rolls are rich in protein, vitamins, mineral elements, dietary fibers and other nutrient elements required by a human body; the algae powder and the flour are fermented together under the action of yeast, so that rich nutritional ingredients are generated through decomposition, and meanwhile, the algae fishy smell can be removed.

Resumen de: AU2021232694A1

The present disclosure relates to the field of grouper cultivation, in particular to a method for hatching and culturing grouper using chlorella. The present disclosure applies the self-produced natural green microalgae product to the indoor industrialized culturing of grouper, breaking through the problems of extremely low hatching survival rate of grouper and serious pollution, and opening up the whole process of indoor industrialized culturing, which provides a boost for the transformation to indoor industrialized culturing of grouper. - 23 -

Resumen de: US2023013336A1

A method of generating a variant cyanobacterium (e.g., Synechococcus elongatus, in particular S. elongatus sp. PCC11801) for photoautotrophic production of an amino acid (e.g., L-phenylalanine); the variant so produced; a method of extending growth of a culture of a variant cyanobacterium and its photoautotrophic production of an amino acid; and a method of photo-autotrophically producing L-phenylalanine.

Resumen de: WO2023287381A1

The invention relates to a method of obtaining and producing an antimicrobial bioactive extract from nitrogen stress-grown microalgae. Antimicrobial activity study is carried out with the antimicrobial bioactive extract obtained from the microalgae genus Auxenochlorella protothecoides in the said invention.

Resumen de: CN115612602A

The invention discloses a light algae ecological water oxygen content regulation and control device which comprises a culture cylinder, a light source assembly is arranged on the culture cylinder, a pneumatic piston assembly is arranged at the bottom of the culture cylinder and comprises an inflation and suction dual-function air pump, and a piston plate is arranged in the culture cylinder in a sliding mode; an air box is arranged at the top of the breeding barrel, and a carbon dioxide introduction mechanism and an oxygen discharging mechanism are arranged on the air box. The piston plate reciprocates to push algae liquid to circularly flow, oxygen generated by photosynthesis of microalgae in the algae liquid is conveniently transmitted into the gas box, when the oxygen concentration in the gas box exceeds a set value, the piston plate can be automatically adjusted to move in an accelerated mode, and the piston plate can be linked with the oxygen discharging mechanism to move in an accelerated mode when moving in the accelerated mode. Therefore, oxygen generated in the gas tank can be exhausted in an accelerated manner, regulation and control are realized, and the situation that when the content concentration of oxygen mixed gas in the gas tank is too high, the respiration of microalgae is too strong, too much oxygen is consumed and too much carbon dioxide is generated is avoided.

Resumen de: CN115612620A

The invention relates to an oil-producing microalgae culture and extraction technology, in particular to a microalgae culture and enzymolysis method, an application of an enzymolysis product and a grease extraction method. According to the method, the cattle urine (CU) and the water are used as the culture medium, so that the growth of the oil-producing microalgae can be effectively promoted, an important theoretical basis is provided for the development and industrial production of biological energy sources, the production cost of the microalgae is reduced, the problem of environmental pollution caused by animal husbandry excrement is relieved, and the method has great significance. According to the method, an enzymolysis method is ingeniously applied, and the use of organic reagents in a traditional extraction method is reduced to the maximum extent. The cost of oil extraction is reduced, and the utilization rate of the microalgae is also improved. The microalgae subjected to enzymolysis has relatively strong antioxidant activity and can be used as a raw material of food additives, medicines, cosmetics and the like, so that comprehensive utilization of the microalgae is really realized. The whole process from the raw materials to the production process to the product is economical and environment-friendly, and the method has great guiding significance in development and application of clean energy.

Resumen de: CN115611431A

The invention relates to the field of algae treatment, in particular to a treatment system and method for precisely controlling algae through biological manipulation, the treatment system comprises an online monitoring part and a biological algae control part, the online monitoring part comprises 5G hyperspectral online monitoring equipment, and the biological algae control part comprises 5G hyperspectral online monitoring equipment; the biological algae control part comprises an ecological soft enclosure, a high-density fish culture module, an ecological floating bed water purification module and precise aeration equipment; by using the on-line monitoring part, an area where cyanobacterial blooms need to be emphatically treated in a lake can be accurately positioned, meanwhile, the risk level of cyanobacterial blooms is accurately recognized, and response measures are rapidly taken in time, so that cyanobacterial treatment is more targeted, higher in accuracy and more obvious in treatment effect, and the economic benefit is increased. Meanwhile, ecological collection, treatment and resource utilization are carried out on excrement of fishes, environment friendliness is achieved, secondary pollution is reduced to the maximum extent, solar energy is adopted as direct power for equipment operation by using online monitoring equipment, cost is saved, and economical and reliable effects are achieved.