Resumen de: WO2023014294A2

This disclosure relates to a recirculating algal-bacterial symbiosis (RABS) system. The RABS system comprises a bacterial bioreactor (BBR) and an algal bioreactor (PBR) that are connected via a recirculation system comprising a first, unidirectional recirculation channel for conveying a liquid stream from the BBR to the PBR, and a second, unidirectional recirculation channel for conveying a liquid stream from the PBR to the BBR. This enables simultaneous treatment of wastewater by the bacteria while culturing algae or microalgae in the PBR.

Resumen de: US2023045512A1

The Biopowerplant is a system that integrates the generation of carbon-neutral energy through the cultivation and conversion of microalgal biomass, with sewage sanitation and environmental carbon recovery, with the additional and secondary production of biofertilizer, biofuel, water for reuse. This system integrates a suboptimal anaerobic digestion subsystem focused on the generation of biogas, the processing of the resulting digestate through a microalgal consortium culture subsystem with biofilm induction and smooth decreasing gradient of light radiation, and the transformation of the generated microalgal biomass into syngas through a subsystem of evaporation, torrefaction, pyrolysis, gasification, and combustion in separate chambers. The syngas and methane from the biogas are subsequently used as fuel in an electric power generator capable of operating with mixed gases. The biogas generation process is enriched through the recirculation of the microalgal biomass supernatant, the residual heat from the syngas generation subsystem, and the heat transferred from the combustion gases of the electric generator. The residual sludge from the biogas generation subsystem is recirculated towards a longitudinal biopile subsystem, where it acts as an anaerobic medium compared to the aerobic medium that constitutes the concentrated microalgal biomass, and both streams are mixed to be transformed into the syngas generation subsystem. Input inflows for system operation are mainly sewage,

Resumen de: AU2021300276A1

Some embodiments include a microalgae culturing system including a bioreactor adapted to propagate microalgae in a culture solution using in combination at least one of natural and artificial light, and at least one nutrient including at least a carbon source, where the microalgae are freely suspended in and form part of the culture solution. A microalgae feed source is coupled to the bioreactor and a first controller between a water conditioning assembly and the bioreactor. The water conditioning assembly is coupled as an input of supply water to the bioreactor, and configured to condition the supply water to a specified purity that enables substantially unhindered growth of the microalgae in the culture solution to a specified concentration, and the first controller is configured to control supply of the microalgae feed source to the bioreactor.

Resumen de: WO2021195712A1

The technology relates to a method for producing a transformed and fully-segregated cyanobacteria, the method comprising incubating the cyanobacteria and a nucleic acid comprising a selectable marker under conditions suitable for transformation of the cyanobacteria with the nucleic acid; further incubating the cyanobacteria in growth media under conditions suitable for recovery of the cyanobacteria; and selecting the transformed and fully-segregated cyanobacteria using a selection agent.

Resumen de: EP4130273A1

The present invention relates to recombinant microalgal cells and their use in heterologous protein production, methods of production of heterologous polypeptides in microalgal extracellular bodies, microalgal extracellular bodies comprising heterologous polypeptides, and compositions comprising the same.

Resumen de: CN218435662U

The utility model provides a microalgae culture device for riverway water body remediation. The microalgae culture device comprises a culture device main body, a stirring structure and an illumination mechanism, the culture device main body comprises a box body, a culture cavity is formed in the box body, and the box body is provided with an air hole and a feeding opening; the illumination mechanism comprises an inner concave part and a culture lamp part, the inner concave part is formed by sinking the bottom wall face of the box body towards the interior of the culture cavity, the inner concave part is made of a transparent material, the inner concave part is provided with a downward first opening, an assembly cavity is formed in the inner concave part, and an illumination part of the culture lamp part extends into the assembly cavity through the first opening; the rotating part of the stirring structure extends into the culture cavity, and the concave part is far away from the rotating area of the rotating part of the stirring structure. Compared with the prior art, the stirring structure can slowly drive the repairing microalgae in the culture cavity to move in the culture cavity at a constant speed, so that all the repairing microalgae have the time of approaching the culture lamp part, all the repairing microalgae can obtain sufficient illumination, and the culture efficiency is improved.

Resumen de: CN115678785A

The invention belongs to the technical field of microalgae biological culture, and discloses a food-grade chlorella culture medium and a culture method. According to the chlorella culture medium disclosed by the invention, a food-grade photoautotrophic culture medium is taken as a basis, waste yeast liquid and grain washing liquid recovered in a beer brewing process are fed into the photoautotrophic culture medium and are respectively used as an organic nitrogen source and a carbon source for culturing chlorella, and photoautotrophic and heterotrophic mixotrophic mode culture is carried out; the food-grade chlorella which is high in biomass, high in protein, safe and pollution-free can be obtained. The cyclic utilization of beer brewing waste yeast and saccharification and grain washing wastewater can be realized, and the environmental pollution is reduced.

Resumen de: CN115678745A

The invention relates to the technical field of coupling microalgae cultivation, in particular to a coupling microalgae air purification cultivation device which comprises a cultivation box, a plurality of universal wheels and an air inlet cylinder, the universal wheels are symmetrically arranged on the cultivation box, the air inlet cylinder is arranged on the cultivation box and communicated with the cultivation box, and the cultivation device further comprises a filter screen, an air inlet pipe and an air outlet pipe. The air inlet pipe is annular and is movably arranged in the air inlet cylinder; the mounting frame is movably arranged in the air inlet cylinder and is connected with the filter screen; the power element is arranged in the air inlet cylinder and is connected with the mounting frame; the air conveying module is arranged on the breeding box and used for conveying air into the breeding box; and the dust removal module is arranged in the air inlet cylinder and is connected with the air conveying module. According to the cleaning device, dust or impurities on the surface of the filter screen can be cleaned, dust attached to the brush can be discharged into the water tank instead of workers, the workers do not need to clean the brush regularly, and the input amount of labor force is reduced.

Resumen de: CN218435695U

The utility model discloses a shaking type microalgae culture system which comprises a flexible transparent cylinder belt, the flexible transparent cylinder belt is basically horizontally arranged, liquid blocking devices are arranged at the two ends of the flexible transparent cylinder belt, and width limiting devices are arranged on the two sides of the flexible transparent cylinder belt. The liquid is stored between the liquid blocking devices at the two ends, the width of the liquid is limited by the width limiting device, the upper side wall of the flexible transparent barrel belt is connected with a transmission rope and is hoisted, and the transmission rope is connected with a shaking device. According to the system, the plastic barrel belt is connected with the shaking device, the barrel belt and algae liquid are guided to move through the shaking device, and the system has the advantages of being good in overall stirring effect, obvious in vertical turning effect, soft in stirring action, labor-saving, low in cost and the like.

Resumen de: CN115677054A

The invention discloses a bacteria-algae photobioreactor which comprises an adjusting tank, a bacteria-algae mixing reactor and a microalgae recycling tank, the regulating tank is connected with a domestic sewage source and is used for collecting domestic sewage and regulating physicochemical properties of the domestic sewage to be suitable for growth of bacteria and algae; the bacteria-algae mixing reactor comprises a transparent columnar reaction container; the adjusting tank is connected with the reaction container through a first pipeline; and a first lifting pump and a first stop valve are mounted on the first pipeline. According to the bacteria-algae photobioreactor, microalgae does not need to be solidified, the microalgae can be directly inoculated into domestic sewage, the contact area is large, and the treatment efficiency is high. The method comprises the following steps: treating organic matters, N and P in sewage through bacteria and algae; according to the invention, the sewage is used as a raw material, then the microalgae is recycled through the microalgae recycling box through electric flocculation, the sewage is further treated in the microalgae recycling process through electric flocculation, suspended particles can be settled by destroying or neutralizing the repulsive force of particles suspended in water in the sewage, and some heavy metal ions can also be removed.

Resumen de: CN218435690U

The utility model provides a screening box which comprises a liquid storage box, cultivation liquid is stored in the liquid storage box, a cultivation box is fixedly installed on the liquid storage box, a box cover is fixedly installed above the cultivation box through screws, a plurality of fluorescent lamps are embedded in the lower surface of the box cover, and a plurality of cultivation assemblies are installed in the cultivation box at intervals. The multiple cultivation pipes are embedded in the cultivation box at intervals, one ends of the multiple cultivation pipes are jointly connected with an air inlet pipe and a liquid inlet pipe through three-way pipes, the liquid inlet pipe is communicated with the micro water pump, the micro water pump is fixedly installed on the liquid storage box, and the liquid inlet end of the micro water pump is communicated with the liquid storage box. The other ends of the cultivation pipes are jointly connected with a liquid return pipe through a connector, the liquid return pipe is communicated with the liquid storage tank, the cultivation pipes are located in the cultivation tank, and ventilation pipes are arranged at the ends, close to the connector, of the cultivation pipes. The screening box provided by the utility model can be used for automatically and circularly supplying a culture solution, is uniform in illumination distribution and is convenient for culturing microalgae.

Resumen de: CN115678802A

The invention discloses stenotrophomonas capable of degrading microcystic toxins, an immobilized microbial agent and application, and particularly relates to stenotrophomonas geniculate DMC-X3 which is obtained by separating and screening a surface water body sample of cyanobacterial blooms in a south reservoir in south Jingxian county in Zhangzhou City of Fujian Province. The strain DMC-X3 has good degradation performance on both low-concentration MC-LR and high-concentration MC-LR, and the degradation rate is gradually increased along with the increase of the inoculum size of the DMC-X3. Meanwhile, the DMC-X3 can efficiently degrade the microcystic toxins under the environmental pressure of extreme pH and temperature. According to the invention, sodium alginate and calcium chloride are used as immobilization materials to immobilize DMC-X3, and the obtained immobilized microbial inoculum can repeatedly and efficiently degrade microcystic toxins for multiple times.

Resumen de: WO2023008787A1

Disclosed herein are a microalga-derived exosome mimetic and a manufacturing method therefor. The method for manufacturing an exosome mimetic of the present invention comprises a step of exclusion processing a buffer solution containing microalgae to prepare nanovesicles. The present invention has the advantage of being able to mass-produce exosome mimetics derived from microalgae on the basis of an extrusion processing method. In particular, the present invention can increase the yield of vesicle production of exosome mimetics derived from microalgae by selectively applying a biolipid surfactant that enhances extrusion processing efficiency.

Resumen de: WO2023006827A1

The invention relates to a method of making a product for human consumption which comprises partially lysed microalgae, said method comprising a) preparing a suspension of microalgae, wherein the microalgae belong to a phylaselected from Chlorophyta, Ochrophyta, and Heterokonta; b) applying a pulsed electric field to the suspension of microalgae, wherein said pulsed electric field has a specific energy input between 25 to 150 kJ per kg suspension (kg sus-1); c) optionally treating the microalgae with enzyme; d) incubating the biomass suspension at 300 rpm, 4-37 °C for 6-48 h e) forming a biomass of partially lysed microalgae; and f) adding the biomass of partially lysed microalgae to a product for human consumption.

Resumen de: WO2023008787A1

Disclosed herein are a microalga-derived exosome mimetic and a manufacturing method therefor. The method for manufacturing an exosome mimetic of the present invention comprises a step of exclusion processing a buffer solution containing microalgae to prepare nanovesicles. The present invention has the advantage of being able to mass-produce exosome mimetics derived from microalgae on the basis of an extrusion processing method. In particular, the present invention can increase the yield of vesicle production of exosome mimetics derived from microalgae by selectively applying a biolipid surfactant that enhances extrusion processing efficiency.

Resumen de: CN218393090U

The utility model discloses a device for reducing carbon and fixing nitrogen by culturing microalgae with waste gas and waste water, which comprises a photobioreactor, a microalgae collector, a purified waste water recoverer and a dryer, the microalgae collector comprises a cavity, a filter barrel and a second pipeline, the filter barrel is arranged in the cavity and can rotate, a material inlet is arranged at the top end of the microalgae collector, and a material outlet is arranged at the bottom end of the second pipeline. A liquid outlet of the photobioreactor is communicated with the material inlet, one end of the second pipeline is communicated with the material inlet, and the other end of the second pipeline is introduced into the filter vat; a material outlet communicated to the outside of the cavity is formed in the bottom of the filtering barrel, and a first water outlet is formed in the bottom of the cavity. The device disclosed by the utility model can integrate wastewater purification, CO2 emission reduction and microalgae culture, and reduces the concentration of nitrogen and phosphorus nutritive salts in wastewater while absorbing CO2 in flue gas through microalgae culture. On one hand, loss of municipal wastewater and nutritive salt is avoided, resource utilization of waste gas and wastewater is fully achieved, and on the other hand, economical utilization of effectively recycled microalgae is achieved.

Resumen de: CN218404163U

The utility model discloses an annular microalgae cultivation pond which comprises a cultivation pond body, a servo motor is installed in the middle of the surface of the bottom of the cultivation pond body, the output end of the servo motor penetrates through the surface of the bottom of the cultivation pond body to be fixedly connected with a rotating rod, and stirring blades are arranged on the rotating rod. Heating pipes are installed on the two sides of the inner wall of the cultivation pool body, and supporting plates are arranged on the surfaces of the two sides of the cultivation pool body. According to the microalgae cultivation pool, a servo motor is turned on, the servo motor drives stirring blades on a rotating rod to slowly stir, a nutrient solution in the cultivation pool body can be effectively stirred, then heating pipes on the two sides of the inner wall of the cultivation pool body are used, the interior of the cultivation pool body can be evenly heated in an omnibearing mode, and the living cultivation environment of microalgae is improved; the microalgae can swim in the culture pond and are prevented from adhering to the inner wall of the culture pond body, the activity of the microalgae in the culture pond body is conveniently improved, and therefore the survival rate of the microalgae is conveniently increased.

Resumen de: CN115651925A

The invention discloses a mutagenesis and screening method for improving astaxanthin produced by haematococcus pluvialis, which comprises the following steps: (1) mutagenizing haematococcus pluvialis solution by using an ARTP mutagenesis method, and observing the growth condition of the haematococcus pluvialis by combining with a microscope to determine optimal process parameters of ARTP mutagenesis; (2) mixing the mutagenized microalgae solution with a protection solution, and culturing in a solid culture medium; (3) selecting the cultured haematococcus pluvialis strains, continuously carrying out pore plate culture on the obtained single strains, and then carrying out multi-index two-step screening according to the algae biomass, the specific growth rate, the astaxanthin content and the oxidation resistance; and (4) carrying out enlarged culture on the screened mutant strain to obtain the target algae strain meeting the requirements. According to the method, double screening indexes reflecting the yield and quality of astaxanthin and a two-step screening procedure are adopted, and the problems that in the haematococcus pluvialis strain screening process, the workload is large, the number of screening indexes is small, and potential mutation strains are likely to be lost can be effectively solved.

Resumen de: RO137238A2

The invention relates to a process for treating residual flows in dairy industry. According to the invention, the process consists in cultivating the Nannochloris sp. microalgae strain in a mixotrophic regime, by using 2.5...10 g/L lactose from whey obtained as a secondary flow during cheese-making, as a source of organic carbon and bubbling of air enriched with 7% CO2 as a source of inorganic carbon, in a photobioreactor equipped with an adjustable lighting source, thermostating and gas bubbling system, in alternating cycles of 12 h of natural/artificial lighting and 12 h of dark, at a temperature of 28 +/-1°C, with the addition of a whey volume corresponding to a concentration of 2.5...10g/L lactose, related to the total volume of nutrient medium, after 7 days of cultivation there resulting a 99...100% reduction of lactose content, up to 96% reduction of chemical oxygen consumption, up to 91% reduction of nitrogen content and 70% reduction of phosphorus content, respectively, and an increase of microalgae biomass quantity of up to 138%.

Resumen de: WO2023001894A1

Provided are compositions containing extracellular vesicles from microalgae (MEVs) that are exogenously loaded with bioactive cargo or other cargo of interest, such as for therapeutic, industrial, diagnostic, and cosmetic uses. The MEVs have a variety of applications as therapeutics, including as vaccines, as anti-cancer therapeutics, as diagnostics, and other such uses.

Resumen de: WO2023001967A1

The present invention relates to a method of preparation of comprising microalgae, in particular Chlorella and products resulting thereof.

Resumen de: US2023025620A1

Extracellular vesicles derived from native, photosynthetic, non-fermenting microalgae are provided. A method for isolating extracellular vesicles from native, photosynthetic, non-fermenting microalgae involving growth, centrifugation and ultracentrifugation steps is also provided. Use of the isolated extracellular vesicles as carriers for deliverling diagnostic, therapeutic, nutraceutic and/or cosmetic agents is further provided.

Resumen de: US2023022786A1

A food composition in the form of an oil-in-water emulsion, comprising 66 to 87 wt % of vegetable oil, less than 1 wt % of microalgal oil, water, microalgal protein, and wherein the composition has a pH of from 2 to 4.8, and wherein the composition is free from egg-derived ingredients.

Resumen de: EP4122330A1

The present invention relates to a method of preparation of comprising microalgae, in particular Chlorella and products resulting thereof.

Resumen de: CN115636864A

The invention provides a method for extracting protein by wall breaking of microalgae, which comprises the following steps: screening a target algae raw material, preparing the raw material subjected to wall breaking treatment, and adjusting the moisture content in the target algae raw material to prepare a microalgae sample; after the temperature of the to-be-frozen area and the temperature of the melting area are adjusted to set values through a refrigerating machine, the microalgae samples are placed on a rotating conveying belt, so that the microalgae samples are rapidly frozen in the freezing link and then enter a rapid melting area along with operation of the conveying belt; the molten microalgae sample is conveyed to an extraction section for protein extraction, a refrigerant in a refrigerator absorbs heat after expansion, and a low-temperature environment is provided for the freezing area; and repeating the steps to form a complete protein extraction process. According to the invention, the frozen algae are quickly melted by using heat generated in the refrigeration process of the refrigerator, so that the energy is saved, the production of the freezing and quick melting section process is coherent, the process is simplified, and the efficiency is improved.