Resumen de: CN115851690A

The invention relates to the technical field of antibiotic enzymes, in particular to a hybrid antibiotic enzyme for killing animal-derived pathogenic bacteria and application of the hybrid antibiotic enzyme. The hybrid antibiotic enzyme comprises a hybrid protein E1C1, and the hybrid protein E1C1 comprises a catalytic domain E1 as shown in SEQ ID NO: 2 and a binding domain C1 as shown in SEQ ID NO: 3. The invention also provides an application of the hybrid antibiotic enzyme in preparation of a pharmaceutical composition for controlling microbial infection, and a pharmaceutical composition with effective components comprising the hybrid antibiotic enzyme. The hybrid antibiotic enzyme disclosed by the invention can be used for rapidly cracking common animal-derived pathogenic bacteria such as escherichia coli, salmonella, staphylococcus aureus and helicobacter pylori, and also can be used for efficiently killing clinically separated common pathogenic bacteria such as acinetobacter baumannii, pseudomonas aeruginosa and klebsiella pneumoniae.

Resumen de: CN115851535A

The invention discloses a lactobacillus rhamnosus WFP52 with an effect of regulating immunity and application of the lactobacillus rhamnosus WFP52, and belongs to the technical field of microorganisms. The classification name of the lactobacillus rhamnosus WFP52 is Lacticaseibacillus rhamnosus, the lactobacillus rhamnosus WFP52 is preserved in the Guangzhou Microbial Culture Collection Center, and the preservation number of the lactobacillus rhamnosus WFP52 is GDMCC No.62580. The lactobacillus rhamnosus WFP52 is named as Lacticaseibacillus rhamnosus WFP52. The strain has excellent low after-acid characteristic and normal-temperature storage stability, not only is beneficial to improving the fermentation characteristic of the sour milk product, but also can adapt to long-time storage under the normal-temperature condition, thereby bringing much convenience to links such as production, storage, transportation and consumption. The strain also has a good inhibition effect on salmonella enteritidis, listeria monocytogenes, staphylococcus aureus and escherichia coli. Meanwhile, the strain can significantly improve the secretion level of cell factors IL-8, IL-10 and IL-12, and has the immunoregulation ability. Therefore, the method has wide application prospects in food, medicine and health care product industries.

Resumen de: BR102021018518A2

KIT PARA A DETECÇÃO DE RESPOSTAS IMUNES CONTRA A BACTÉRIA SALMONELLA ENTERICA (SALMONELA). Um kit para a detecção de respostas imunes contra a bactéria Salmonella enterica (salmonela) com lipopolissacarídeo (LPS) ou subunidades deste de salmonela imobilizado em substrato sólido; e flagelina ou subunidades desta de salmonela imobilizada em substrato sólido. Um kit para a detecção de respostas imunes contra a bactéria Salmonella enterica (salmonela) produzido por várias etapas sequenciais. Um kit para a detecção de respostas imunes contra a bactéria Salmonella enterica (salmonela) configurado para diferenciar entre animais infectados ou vacinados contra salmonela há período entre 1 dia e 21 dias ou há períodos superiores a este.

Resumen de: CN115856297A

The invention provides a preparation method of a kit for detecting salmonella typhimurium and the kit, and belongs to the technical field of kits. The preparation method comprises the following steps: firstly, preparing magnetic beads with superparamagnetism, coating the magnetic beads serving as a magnetic core with an organic covalent skeleton (COF) to form a core-shell structure, namely MCOF nano magnetic particles, and then loading gold nanoparticles on the MCOF nano magnetic particles by using an in-situ reduction method, so as to obtain the MCOF nano magnetic particles. And finally, coupling with an aptamer of salmonella typhimurium to synthesize the nano probe apt-MCOF-AuNPs with high fluorescence emission. The salmonella typhimurium and the nanoprobe are co-incubated to form a probe-thallus compound, a quenching agent Fe < 3 + > is added to quench fluorescence of the probe, the fluorescence of the probe cannot be quenched by the Fe < 3 + > due to the protective effect of thallus on the nanoprobe and competitive binding of the thallus and the Fe < 3 + >, and the solution is bright green fluorescence under irradiation of 365 nm ultraviolet light. Therefore, rapid and specific detection of salmonella typhimurium is realized.

Resumen de: CN115851988A

The invention relates to the technical field of microbiological detection, and particularly discloses a multiple PCR detection primer combination for bacteria and application thereof, and the multiple PCR detection primer combination for bacteria comprises primer combinations for performing multiple PCR detection on pseudomonas aeruginosa, staphylococcus aureus, klebsiella pneumoniae, proteus mirabilis, helicobacter and salmonella respectively. The invention also provides a multiple PCR detection kit for bacteria and a multiple PCR detection method for bacteria. The detection primer combination disclosed by the invention has good sensitivity and specificity, is matched with an optimized amplification system and program, so that the amplification efficiency is remarkably improved, the result is accurate, the efficiency is high, the consumed time is short, the damage to animals can be avoided, and the detection primer combination has a practical application value.

Resumen de: CN115851771A

The invention discloses a salmonella gallinarum attenuated isolated strain not expressing Peg mycosis and application of the salmonella gallinarum attenuated isolated strain. The isolated strain is orally inoculated with an SGOP strain with the dose of 1 * 10 < 10 > CFU and has no pathogenicity to SPF chickens of 1 day age, the SGOP strain is immunized and inoculated to the SPF chickens of 1 day age, chicken flocks immunized with the SGOP strain can resist toxic attack of salmonella gallinarum wild strain U20 after 21 days, and 70% of chicken flocks attacked with toxic attack are protected without diseases. The SGOP strain disclosed by the invention is relatively high in safety, does not influence the monitoring and purification procedures of avian salmonella, and has relatively great potential in clinical prevention and control of salmonella gallinarum infection and marker attenuated vaccine candidate strains thereof.

Resumen de: CN115850407A

The invention belongs to the technical field of germ detection, and particularly relates to an antigen protein and application thereof, and the antigen protein is suitable for salmonella pullorum detection. The antigen protein comprises a sequence as shown in SEQIDNO.1 or SEQIDNO.2, the antigen protein has very strong specificity to salmonella pullorum antibodies, the salmonella pullorum is detected by an indirect ELISA (Enzyme-Linked Immunosorbent Assay) method taking the two antigen proteins as coating antigens, the minimum positive serum dilution ratio can reach 1: 1600, and the sensitivity is high; in addition, the intra-batch repetition variable coefficient and the inter-batch repetition variable coefficient are both lower than 10%, and repeatability is good.

Resumen de: CN115851990A

The invention discloses a primer and a kit for rapidly detecting salmonella and application of the primer and the kit. The detection kit is composed of a nucleic acid rapid amplification reagent, fluorescence immunochromatography test paper and an operation buffer solution. The nucleic acid rapid amplification reagent comprises an amplification buffer solution containing graphene, a salmonella specific amplification primer and a quality control product. The fluorescence immunochromatography test paper is composed of a sample pad, a combination pad coated with a fluorescent microsphere antibody (a marker at one end of an anti-DNA amplification product) conjugate, an NC film containing a detection line (a marker at the other end of the anti-DNA amplification product) and a control line, absorbent paper and a PVC rubber plate. And after the rapid nucleic acid amplification reaction is completed, adding an operation buffer solution, uniformly mixing, dropwise adding to a test paper sample adding window, and carrying out fluorescence signal interpretation by using a fluorescence analyzer after 2 minutes. And the whole process can be completed within 30 minutes. The salmonella rapid detection kit is simple, convenient, rapid and sensitive to operate, reliable in result and beneficial to practical application.

Resumen de: CN115851500A

The invention discloses a strain of lactobacillus plantarum (Lactobacillus plantarum) CC0907 and an application of the lactobacillus plantarum CC0907. The strain is separated and screened from the intestinal tract of the bufo bufo gargarizans collected from the natural reserve, grows well on an MRS agar culture medium, does not have a hemolysis phenomenon, is sensitive to various antibiotics, does not carry antibiotic resistance genes, has better endurance capacity to acid, cholate, artificial gastric juice and artificial intestinal juice, has strong adhesion capacity to human colon cancer cells HT-29, and can be used for preparing the antibiotic-resistant strain for the bufo bufo gargarizans of the bufo bufo gargarizans of the bufo bufo gargarizans. The lactobacillus plantarum has the advantages that the lactobacillus plantarum is high in lactic acid and acetic acid production capacity, has high inhibition capacity on staphylococcus aureus, salmonella, escherichia coli, pseudomonas aeruginosa, staphylococcus haemolyticus, pasteurella multocida and the like, has the potential of reducing blood sugar and cholesterol, and has good prevention and treatment effects on a DSS-induced mouse colitis model; the inflammation index is obviously reduced. Therefore, the strain can be applied to the field of human and animal functional foods.

Resumen de: CN115838720A

The invention discloses an attenuated salmonella vector carrying shSTAT3/shPD-L1 recombinant plasmids and application of the attenuated salmonella vector, and belongs to the technical field of biology. According to the invention, two promoters are adopted to respectively express two shRNAs in the same expression vector, so that an attenuated salmonella vector carrying shSTAT3/shPD-L1 recombinant plasmid is successfully constructed; the shSTAT3/shPD-L1 recombinant plasmid can silence expression of STAT3 and PD-L1 in colorectal cancer cells at the same time, so that proliferation and migration of cancer cells are inhibited, cell apoptosis is promoted, and cell cycle arrest is caused; the shSTAT3/shPD-L1 recombinant plasmid carried by the attenuated salmonella can obviously inhibit the growth of C57BL/6N mouse in-situ colorectal cancer through the mechanisms of inhibiting cell proliferation, promoting apoptosis, increasing immune cell infiltration and the like, has a good anti-tumor effect, and becomes an effective new strategy for treating colorectal cancer.

Resumen de: CN115838786A

The invention discloses a detection technology for quantitative viable bacteria RNA single nucleotide polymorphism, and belongs to the field of nucleic acid detection. The method integrates nucleic acid sequence dependent amplification techniques (NASBAs), allowing rapid and specific generation of target RNA amplification, as well as amplification hindering mutation systems (ARMSs), by introducing mismatch at the 3'end basic group of the forward primer, the specificity of identifying single nucleotide is enhanced, so that high-selection identification is performed on RNA mutation, single nucleotide polymorphism with abundance as low as 0.5% can be sensitively identified, and salmonella viable bacteria as low as 80 CFU can be detected. The technology allows RNA amplification under an isothermal condition, single nucleotide polymorphism on RNA is detected in a single tube, the technology can be used for evaluating existence and activity of drug-resistant bacteria under the action of a disinfectant, and the application prospect is good.

Resumen de: CN115838813A

The invention provides a salmonella virulence gene detection primer group based on a fluorescent PCR technology and a kit, and belongs to the technical field of pathogen detection. The invention provides a salmonella virulence gene detection primer group based on a fluorescent PCR (Polymerase Chain Reaction) technology. The salmonella virulence gene detection primer group comprises a primer group for respectively amplifying ssaR, spvC, pefA, sipA and fimA virulence genes and a primer group for respectively amplifying sifA, sopE2, sopB, prgH and stn virulence genes. Compared with other primer groups, the primer group not only can accurately obtain a melting curve peak amplified by a target virulence gene, but also has specificity, meanwhile, the melting curve peaks in pairs are not mutually overlapped, and whether 10 salmonella virulence genes exist or not can be accurately, specifically and simultaneously detected. The invention provides a new means for rapidly detecting the virulence genotype of salmonella.

Resumen de: WO2023044419A2

Recombinant polypeptides that include a chimeric secretory component (cSC) protein in which the D2 domain of secretory component is modified to confer one or more non-native properties to the polypeptide are described. The D2 domain can be modified, for example, to confer specific binding to a target molecule, such as by replacing the D2 domain with a single domain antibody or by modifying the D2 domain by replacement of complementarity determining region (CDR)-like loops with CDR sequences from a single domain antibody. The D2 domain can also be modified to enable fluorometric or colorimetric detection of the recombinant polypeptide. Methods of using the recombinant polypeptides, such as for treating or inhibiting a microbial infection, are also described. In some examples of these methods, the D2 domain of the recombinant polypeptide is modified to confer specific binding to a microbial antigen.

Resumen de: US2023086107A1

The present disclosure belongs to the technical field of pathogen detection, in particular to loop-mediated isothermal amplification (LAMP) primer sets for detecting porcine susceptibility-related pathogenic bacteria, and a kit, a LAMP chip and use based on the same. The LAMP primer sets for detecting porcine susceptibility-related pathogenic bacteria include an Actinobacillus pleuropneumoniae primer set, a Haemophilus parasuis primer set, a Salmonella choleraesuis primer set, a Bordetella bronchiseptica primer set, a Pasteurella multocida primer set, a Streptococcus suis primer set, and an Erysipelothrix rhusiopathiae primer set.

Resumen de: AU2021324883A1

Provided are attenuated immunostimulatory bacteria with genomes that are modified to, for example, reduce toxicity and improve the anti-tumor activity, such as by increasing accumulation in the tumor microenvironment, particularly in tumor- resident myeloid cells, improving resistance to complement inactivation, reducing immune cell death, promoting adaptive immunity, and enhancing T-cell function. Also provided are immunostimulatory bacteria for use as vaccines, and for delivery of mRNA. The increase in colonization of phagocytic cells improves the delivery of encoded therapeutic products to the tumor microenvironment and to tumors and permits, among other routes, systemic administration of the immunostimulatory bacteria. The increase in colonization of phagocytic cells also provides for use of immunostimulatory bacteria for direct tissue administration for use as vaccines.

Resumen de: WO2023043986A1

This invention is directed to a product for use in avian subjects comprising a combination of in ovo vaccine and probiotic, and related methods.

Resumen de: US2023090746A1

Helicobacter pylori is a leading cause of gastric mucosal inflammation, peptic ulcers, and gastric adenocarcinoma. Emerging antimicrobial-resistant H. pylori has hampered the successful eradication of frequent chronic infections. Additionally, due to the absence of effective vaccines against H. pylori, a safe vaccine is highly demanded. Disclosed herein are innovative Protective Immunity Enhanced Salmonella Vaccine (PIESV) vector strains to deliver and express multiple H. pylori antigen genes Immunization of mice with a vaccine delivering the HpaA, NapA (also termed Hp-NAP), UreA and UreB antigens, provided sterile protection against H. pylori SS1 infection in 7 out of 10 tested mice. Compared to the control groups that had received PBS or a PIESV with an empty vector, immunized mice exhibited specific and significant cellular recall responses and antigen-specific IgG2c, IgG1, total IgG and gastric IgA antibody titers. Importantly, the mice immunized with the vaccine candidate showed a significant reduction in a load of an unidentified Gram-positive rod-shaped bacteria in their stomach compared to the control groups. In conclusion, a Salmonella Typhimurium-based live vaccine delivering four antigens shows promise as a safe and effective vaccine against H. pylori infection.

Resumen de: CN115820888A

The invention relates to a method for detecting living flora of a biological sample based on a PMA-qPCR technology. The method comprises the following steps: culturing Caco2 cells by using a DMEM (Dulbecco Modified Eagle Medium) culture medium; a Luria-Bertani (LB) culture medium is used for culturing escherichia coli and salmonella, and an MRS culture medium is used for culturing lactobacillus plantarum and enterococcus faecalis; preparing a simple synthetic community; pretreatment and doping culture of a biological sample; carrying out osmotic pressure lysis and PMAxx treatment on the host cells; extracting DNA (deoxyribonucleic acid); synthesizing a primer; carrying out metagenome sequencing; calculating the load and absolute abundance of the microbiota; carrying out bioinformatics analysis on shotgun metagenome data; and statistical analysis. The method disclosed by the invention can be used for effectively detecting the viable flora in biological samples such as saliva, excrement and the like.

Resumen de: CN115820498A

The invention discloses a strain of lactobacillus plantarum YJ2406 and an application of the strain of lactobacillus plantarum YJ2406. The lactobacillus plantarum YJ2406 provided by the invention has the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.25907. The strain is separated from dry pickled Chinese cabbage in Guizhou province, grows well on an MRS culture medium, has no hemolysis phenomenon, is sensitive to various common types of antibiotics, does not carry antibiotic resistance genes, has better endurance capacity to acid, cholate, artificial gastric juice and artificial intestinal juice, has stronger adhesion capacity to human colon cancer cells HT-29, has strong short-chain fatty acid production capacity, and can be used for preparing the antibiotic-resistant strain. The traditional Chinese medicine composition has good in-vitro blood sugar and cholesterol lowering capacity, has strong inhibition capacity on escherichia coli, staphylococcus aureus, salmonella typhimurium, pseudomonas aeruginosa, campylobacter jejuni and campylobacter coli, has good prevention and treatment effects on a DSS-induced mouse colitis model, can significantly reduce inflammation symptoms, and has good clinical application prospects. The intestinal flora structure of the mouse can be effectively adjusted, and the number of lactobacillus is remarkably increased.

Resumen de: CN115820482A

The invention relates to bacillus amyloliquefaciens YN-BA2 as well as a microbial agent and application thereof, and belongs to the technical field of biological control and microorganisms, the bacillus amyloliquefaciens YN-BA2 is classified and named as bacillus amyloliquefaciens and is preserved, and the preservation number is GDMCC No: 62746. The strain has good heat resistance, acid resistance and cholate resistance, has a high inhibition effect on salmonella pullorum and salmonella enteritidis in vitro, can be used for preparing a biological prevention and control microecological preparation, is orally taken to chicken flocks, and is suitable for prevention and control of salmonella pullorum diseases in livestock and poultry industries.

Resumen de: CN115819525A

The invention discloses a tumor-targeting typhoid toxin B subunit mimic peptide and application thereof. And the typhoid fever toxin B subunit mimic peptide is selected from any one of six polypeptides such as YQPB-1: COOH-Thr-Gly-Ser-Gly-Asn-Ala-Asn-Val-NH2, and the like. The invention further discloses a preparation method of the typhoid fever toxin B subunit mimic peptide. The invention further discloses application of the typhoid toxin B subunit mimic peptide or the dimer and the multimer thereof in preparation of a tumor-targeted diagnostic reagent or a tumor treatment medicine. A series of novel mimic peptides simulating typhoid toxin B subunit (PltB) secreted by typhoid salmonella are developed, and the mimic peptides of the YQPB-X series can be specifically combined with tumor cells with high ganglioside expression; in-vivo optical imaging and results prove that the compound has excellent imaging results on various tumors such as neuroblastoma, non-small cell lung cancer, melanoma, breast cancer and osteosarcoma.

Resumen de: CN115812854A

The invention provides a feed additive and application thereof. The feed additive comprises the following components in parts by weight: 35-40 parts of formic acid, 5-10 parts of acetic acid, 15-30 parts of propionic acid, 2-5 parts of octanoic acid, 5-10 parts of chitosan oligosaccharide, 5-10 parts of betaine hydrochloride and 10-20 parts of copper sulfate, and 2-5 parts of chlorine dioxide tablets are added before use. The feed additive provided by the invention has relatively strong bacteriostatic and bactericidal effects. The composition is sprayed and added into the feed polluted by the mould and the salmonella, so that the mould and the salmonella in the feed can be remarkably killed and removed. The composition is provided for salmonella infected broiler chickens, the production performance of the broiler chickens can be remarkably improved, and the number and the bacterium carrying rate of main pathogenic bacteria such as intestinal salmonella, escherichia coli and campylobacter are reduced. The device can be used in the feed industry and used for feed cleaning; the feed antibiotic can also be replaced to be used in healthy breeding of poultry, and intestinal health and antibiotic-free production of the poultry are promoted.

Resumen de: US2023082935A1

This invention is directed to a product for use in avian subjects comprising a combination of in ovo vaccine and probiotic, and related methods.

Resumen de: US2023081896A1

Provided herein is an air monitoring system with a venturi pump including an air supply passageway, a sample passageway, and a discharge passageway, the discharge passageway in fluid communication with the air supply passageway and the sample passageway, and a detection device including a biochip, a light emitting source, a photodetector, and a controller electronically coupled to the photodetector. Also provided herein is a photonic biogel and uses thereof for spectroscopic detection of airborne pathogens.

Resumen de: WO2023039194A1

Provided herein is the ability for intravenous delivery of bacteria without the septic side-effects and for delivery of immune modulating proteins that are systemically toxic to be delivered directly to the tumor micro-environment without any systemic toxicity.