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Method for constructing acid-resistant microalgae based on ferredoxin

Resumen de: CN120485238A

The invention discloses an acid-resistant microalgae construction method based on ferredoxin, and belongs to the field of biology and molecular biology. The method specifically comprises the following steps: taking Phatr3J33543 with a sequence structure as shown in SEQ ID No.1, Phatr3J50516 with a sequence structure as shown in SEQ ID No.2 or Phatr3Jdraft1806 with a sequence structure as shown in SEQ ID No.3 as a target gene, removing a termination codon of the target gene, and connecting a fluorescent protein gene with an initiation codon removed through a DNA (Deoxyribose Nucleic Acid) sequence for coding glycine to obtain a recombinant gene; inserting the recombinant gene into multiple cloning sites of a pPhaNR plasmid to obtain a vector plasmid; introducing the carrier plasmids into diatom cells through electroporation, and inoculating the diatom cells to an ESAW solid selective culture medium containing bleomycin to grow brown algal colonies; selecting the algal colonies with fluorescent protein signals from the algal colonies, namely the acid-resistant engineering algae. Compared with wild algae, the acid resistance of the engineering algae obtained by the invention is obviously improved. The method has important reference significance on acid-resistant diatom cultivation.

Acid-resistant microalgae construction method based on bicarbonate ion transporter protein

Resumen de: CN120485237A

The invention discloses an acid-resistant microalgae construction method based on bicarbonate ion transporter protein, and belongs to the field of biology and molecular biology. The method specifically comprises the following steps: taking Phatr3J33543 with a sequence structure as shown in SEQ ID No.1, Phatr3J50516 with a sequence structure as shown in SEQ ID No.2 or Phatr3Jdraft1806 with a sequence structure as shown in SEQ ID No.3 as a target gene, removing a termination codon of the target gene, and connecting a fluorescent protein gene with an initiation codon removed through a DNA (Deoxyribose Nucleic Acid) sequence for coding glycine to obtain a recombinant gene; inserting the recombinant gene into multiple cloning sites of a pPhaNR plasmid to obtain a vector plasmid; introducing the carrier plasmids into diatom cells through electroporation, and inoculating the diatom cells to an ESAW solid selective culture medium containing bleomycin to grow brown algal colonies; selecting the algal colonies with fluorescent protein signals from the algal colonies, namely the acid-resistant engineering algae. Compared with wild algae, the acid resistance of the engineering algae obtained by the invention is obviously improved. The method has important reference significance on acid-resistant diatom cultivation.

LIQUID COMPOSITION

Resumen de: WO2025166399A1

The present disclosure generally relates to liquid compositions that can be used to treat a water body, for example a dam, river or waste water. In particular, the present disclosure relates to compositions that can be used to control cyanobacteria in a water body. The liquid compositions comprise worm castings, a sugar source and optional other additives/excipients. The present disclosure also relates to methods of treating a water body using the liquid compositions. The present disclosure also relates to processes for preparing the liquid compositions, and also to uses and kits comprising the liquid compositions.

CULTURE METHOD AND CULTURE DEVICE

Resumen de: US2025257313A1

A culture device comprises: a gas supply unit; and a plurality of containers. The gas supply unit is connected with the plurality of containers through a plurality of gas supply lines, respectively. The plurality of gas supply lines are provided with a plurality of valves, respectively. While microalgae are cultured in the plurality of containers, the opening degrees of the plurality of valves are adjusted. By such adjustment of the opening degrees, a greater amount of gas is supplied to one of the plurality of containers than those of the other containers.

MICROALGAE-ENRICHED FOODS

Resumen de: US2025255337A1

In alternative embodiments, provided are compositions, including products of manufacture, foods and drinks and kits, comprising one or mixtures of two or more or a plurality of microalgae, wherein the microalgae, optionally blue-green algae or cyanobacteria, or extracts of the microalgae, or dried extracts of the microalgae, are formulated into a food, candy, nutritional supplement, or an ingestible liquid. In alternative embodiments, the one or two or more or a plurality of microalgae comprise a microalgae from the genus: Spirulina sp., Arthrospira sp., Limnospira sp, Dunaliella salina, and/or Nannochloropsis salina. In alternative embodiments, the one or two or more or a plurality of microalgae are formulated into a food such as a guacamole (to generate for example, to generate a Microalgae-Enriched Guacamole (MEG).

USE OF CYANOBACTERIAL BIOREACTOR FOR CARBON SEQUESTRATION

Resumen de: US2025257372A1

The subject invention provides compositions and methods for reducing deleterious atmospheric gases using microorganisms. In preferred embodiments, a composition comprising one or more beneficial microorganisms convert carbon dioxide to calcium carbonate, magnesium carbonate, a bicarbonate, or a combination thereof. The source of the carbon dioxide can be industrial emissions. In some embodiments, the composition sequesters carbon with the use of a trough growth system and the culture and/or growth by-product can be sequestered in underground geological formations.

Chemical sterilization or disinfection method of microalgae culture medium using calcium hypochlorite

Nº publicación: KR20250122828A 14/08/2025

Solicitante:

경상국립대학교산학협력단