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ROTATABLE POWER SUPPLYING DEVICE USING MICROALGAE CULTURE BUBBLES AND CULTURING APPARATUS COMPRISING THE SAME

Resumen de: KR20250088251A

본 발명은 수조 내에서 부상하는 공기의 부력에 의해 상향이동하는 복수의 바스켓; 상기 복수의 바스켓이 일렬로 장착되며 공기에 의한 부력만으로 상기 바스켓의 상향이동과 함께 회전되는 바스켓 이송수단; 및 상기 바스켓 이송수단의 상단부 및 하단부에 한쌍이 결합되어 함께 회전하여 외부로 동력을 전달하는 회전동력전달부를 포함하는 미세조류 배양 기포를 이용한 수조내 회전동력장치 및 이를 포함하는 배양기를 제공한다.

Quantitative feeding system for preparation of microalgae culture medium

Resumen de: CN120158363A

The invention discloses a quantitative feeding system for preparation of a microalgae culture medium, which relates to the technical field of microalgae culture and comprises a base plate, a vertical plate vertically arranged at the top of the base plate, a microalgae culture tank arranged at the top of the base plate and a culture medium preparation and supply tank, the culture medium step-by-step replacement device and the centrifuge are sequentially arranged on the outer wall of the vertical plate from top to bottom, and the step-by-step dilution device is arranged on the base plate and located at the bottom of the centrifuge. When the microalgae culture medium feeding system is used for feeding a culture medium, the culture medium is supplied in a manner of gradually replacing and adapting the culture medium and thoroughly replacing and diluting the culture medium, so that microalgae can quickly adapt to the solution concentration of a new culture medium, and normal growth and reproduction of the microalgae are facilitated.

Culture Method of Polar Microalgae

Resumen de: KR20250087223A

본 발명은 배양기간에 걸쳐 영양원 또는 영양원 함유 배지를 배양기에 분할투입하여 미세조류를 배양하는 것을 특징으로 하는 극지 미세조류의 배양방법을 제공한다.

Thermal power plant wastewater resource utilization system and method based on microalgae technology

Resumen de: CN120136346A

The invention provides a power plant wastewater resource utilization system based on a microalgae technology, which comprises a wastewater pretreatment unit: a microalgae culture unit which is provided with a culture pond or a reactor, introduces pretreated high-concentration wastewater into the culture pond or the reactor, and inoculates microalgae for culture; the harvesting and extracting unit is used for harvesting the microalgae after the microalgae grow to a certain stage; and the wastewater reflux and reutilization unit is used for further treating the wastewater after microalgae culture, removing residual microalgae cells and metabolites, and then refluxing the wastewater to a power plant for reutilization or up-to-standard discharge. The invention aims to convert inorganic pollutants such as nitrogen, phosphorus and the like in the wastewater discharged by the power plant into valuable biomass resources.

Bile shellfish breeding method and bile shellfish breeding system

Resumen de: CN120130411A

The invention provides a gall shell culture method and a gall shell culture system. The bile shell culture method provided by the invention comprises the following steps: putting culture effluent of a bile shell culture pond into a microalgae treatment pond, culturing chaetoceros mulleri in the microalgae treatment pond, and carrying out primary treatment on the culture effluent to obtain primarily treated tail water; the tail water obtained after primary treatment is put into a secondary treatment pond, probiotics are added, the tail water obtained after primary treatment is subjected to secondary treatment through the probiotics, and tail water obtained after secondary treatment is obtained; the tail water subjected to secondary treatment is put into a gall shell culture pond to be used for culturing gall shells, and water circulation is achieved; wherein chaetoceros mulleri is in logarithmic growth in the microalgae treatment pond, the chaetoceros mulleri is food for breeding the gall-bladder, and ammonia nitrogen in the breeding effluent is removed while the chaetoceros mulleri grows, so that the content of the ammonia nitrogen in the tail water subjected to primary treatment meets the requirement of breeding the gall-bladder; the probiotics are used for removing nutrient substances which cannot be absorbed by the microalgae in the primarily treated tail water.

NdhE mutant and application thereof

Resumen de: CN120137921A

The invention relates to an NAD (P) H dehydrogenase subunit gene ndhE mutant capable of increasing the growth speed of cyanobacteria. A section of amino acid with the sequence shown as SEQ ID NO: 5 is added to the tail of a coded protein; the invention also relates to application of the ndhE mutant in improving the growth speed of cyanobacteria and a constructed fast-growing engineering strain. According to the invention, ndhE of synechococcus PCC 7942 is mutated, so that a section of amino acid with a sequence as shown in SEQ ID NO: 5 is added at the tail of a protein coded by the synechococcus PCC 7942, the obtained mutant strain has a faster growth speed in highlight, and the increase of the growth speed has a synergistic effect with AtpA C252Y mutation. Therefore, the growth speed of the synechococcus can be greatly improved by performing corresponding genetic modification on the synechococcus, and the yield of the microalgae and the target compound can be improved by using the mutant strain as the chassis algae for metabolic modification and production of the target compound.

Microalgae feed and preparation method thereof

Resumen de: CN120130570A

The present invention provides a microalgae feed and a preparation method thereof, and relates to the technical field of animal feeds, the microalgae feed comprises 25% of microalgae powder, 30% of a protein source, 40% of a carbohydrate source and 30% of a mineral substance mixture based on the total weight of the feed, and the mineral substance mixture comprises the following components in percentage by weight: 25% of the microalgae powder, 30% of the protein source, 40% of the carbohydrate source and 40% of the mineral substance mixture based on the total weight of the feed. The content of the mineral substance mixture accounts for 5% of the total weight of the feed, the comprehensiveness and balance of feed nutrition are ensured through the elaborate proportioning of the protein source, the carbohydrate source and the mineral substance mixture, various nutritional requirements of animals in different growth stages are met, healthy growth and development of the animals are promoted, and through the analysis of intestinal flora, the health of the animals is promoted. According to the present invention, the number of the beneficial bacteria such as bifidobacteria and lactic acid bacteria in the animal intestinal tract fed with the microalgae feed is substantially increased, the positive effect is provided on the improvement of the animal intestinal flora, the efficient utilization of the feed reduces the emission of nitrogen, phosphorus and other pollutants in the excrement,

Threonyl-tRNA synthetase mutant and application thereof

Resumen de: CN120137919A

The invention relates to a threonyl-tRNA synthetase mutant, proline at the 499th site of the threonyl-tRNA synthetase mutant is mutated into serine, and a reference sequence of threonyl-tRNA synthetase before mutation is as shown in SEQ ID NO: 2; the invention also relates to application of the threonyl-tRNA synthetase in improving the growth speed of cyanobacteria and a constructed fast-growing engineering strain. According to the invention, the mutation from proline to serine at the 499th site is introduced into the threonyl-tRNA synthetase gene of synechococcus PCC 7942, so that the obtained mutant strain has a faster growth speed in highlight, and the increase of the growth speed has a synergistic effect with the AtpA C252Y mutation. Therefore, the growth speed of the synechococcus can be greatly improved by performing corresponding genetic modification on the synechococcus, and the yield of the microalgae and the target compound can be improved by using the mutant strain as the chassis algae for metabolic modification and production of the target compound.

Preparation of biomass carbon quantum dots and application of biomass carbon quantum dots in microalgae culture

Resumen de: CN120136080A

The invention discloses preparation of biomass carbon quantum dots with pine sawdust as a raw material and an application method of the biomass carbon quantum dots in microalgae culture. According to the method, high-yield biomass carbon quantum dots are synthesized through an alkali-assisted oxidation process, and the blue light emission characteristic of the biomass carbon quantum dots is matched with the absorption spectrum of a microalgae photosynthetic system II (PSII). The method comprises the following specific steps: pretreating pine sawdust, then carrying out hydrothermal reaction to prepare pine derived hydrothermal carbon, and then carrying out alkaline oxidation stripping on the hydrothermal carbon through NaOH and H2O2 to extract the carbon quantum dots. The prepared biomass carbon quantum dots have the advantages of high yield, excellent optical performance, good water solubility and biocompatibility. In microalgae culture, the biomass concentration of microalgae and the synthesis efficiency of protein and lipid can be remarkably improved by adding a proper amount of the carbon quantum dots, and an efficient and environment-friendly solution is provided for waste recycling and microalgae high-value culture.

Raw material composition of thermoplastic product mixed with microalgae, product and preparation method thereof

Resumen de: CN120153024A

The invention relates to a raw material composition of a thermoplastic product mixed with microalgae, the product and a preparation method, the composition comprises polyvinyl alcohol, microalgae, a compatilizer, a plasticizer, a cross-linking agent, a reaction auxiliary agent and a lubricant, the weight percentage of the microalgae is between 10% and 30% based on the weight of the polyvinyl alcohol, and the weight percentage of the compatilizer is between 10% and 30% based on the weight of the plasticizer. The weight percentage of the compatible agent is between 2% and 5% based on the weight of the polyvinyl alcohol, the weight percentage of the plasticizer is between 20% and 30% based on the weight of the polyvinyl alcohol, and the weight percentage of the cross-linking agent is between 0% and 1.5% based on the weight of the polyvinyl alcohol. The weight percentage of the reaction auxiliary agent based on the weight of the polyvinyl alcohol is between 1% and 5%, and the weight percentage of the lubricant based on the weight of the polyvinyl alcohol is between 0.1% and 0.5%.

Culture method of blue-green bacteria

Resumen de: CN120137811A

The invention discloses a culture method of blue-green bacteria. The culture method of the blue-green bacteria comprises the following steps: providing the blue-green bacteria, and carrying out physical mutation treatment on the blue-green bacteria to obtain primary mutation blue-green bacteria. And carrying out chemical mutation treatment on the primary mutated cyanobacteria to obtain secondary mutated cyanobacteria. And carrying out a temperature resistance test and an environment resistance test on the secondary mutation cyanobacteria to obtain the target cyanobacteria. The fatality rate of the physical mutation treatment is 50-80%, and the fatality rate of the chemical mutation treatment is 40-55%. The blue-green bacteria with high temperature resistance and industrial waste gas resistance can be obtained through the culture method of the blue-green bacteria provided by the invention.

Marine microalgae efficient gene editing system as well as construction method and application thereof

Resumen de: CN120137975A

The invention discloses a marine microalgae efficient gene editing system as well as a construction method and application thereof. An efficient'all-in-one 'CRISPR/CAS9 expression vector pKSdiaCas9-sgRNAble is constructed by inserting an expression cassette of a bleomycin resistance gene ble into a pKSdiaCas9sgRNA plasmid, meanwhile, four gRNAs targeting different sites of the same gene are respectively designed to obtain four CRISPR/CAS9'all-in-one' plasmids, and the four CRISPR/CAS9'all-in-one 'plasmids are co-transformed into algae cells, so that the total editing probability and the probability of homozygote mutation are greatly improved. Through optimization of a gene editing tool, improvement of a gene gun transformation method and an identification strategy of a genome level, efficient and stable marine microalgae gene editing is realized, and an important technical support is provided for microalgae gene function research and application.

KaiA gene mutant and application thereof

Resumen de: CN120136984A

The invention relates to a kaiA gene mutant capable of improving the growth speed of cyanobacteria, the 266th-273rd amino acids are shown as SEQ ID NO: 5, and the 274th amino acids and the amino acids behind the 274th amino acids are deleted; the invention also relates to application of the kaiA gene mutant in improving the growth speed of cyanobacteria and a constructed fast-growing engineering strain. According to the invention, the kaiA gene of synechococcus PCC 7942 is mutated, so that the 266th-273rd amino acids of the coded protein are changed, and the 274th and subsequent amino acids are deleted, so that the obtained mutant strain has a faster growth speed, and the increase of the growth speed has a synergistic effect with AtpA C252Y mutation. Therefore, the growth speed of the synechococcus can be greatly improved by performing corresponding genetic modification on the synechococcus, and the yield of the microalgae and the target compound can be improved by using the mutant strain as the chassis algae for metabolic modification and production of the target compound.

METHOD FOR HARVESTING MICROALGAE AND EXTRACTING USEFUL MICROALGAE PRODUCTS USING ULTRATHIN ANISOTROPIC MAGNETIC NANOPARTICLES

Resumen de: KR20250085071A

본 발명은 미세조류가 포함된 용액에 표면에 양전하가 유도된 초박형 자성 나노입자를 첨가한 후 혼합하여 자성 나노입자-미세조류 응집체를 형성하는 제1 단계, 상기 자성 나노입자-미세조류 응집체가 포함된 혼합용액을 초음파 처리하는 제2 단계, 상기 초음파 처리된 혼합용액에 지질추출용매를 처리하여 미세조류가 생산한 유용 물질 및 지질을 추출하는 제3 단계 및 자석을 이용하여 상기 초음파 처리된 혼합용액에서 상기 자성 나노입자를 회수하는 제4 단계를 포함하는 초박형 자성 나노입자를 이용한 미세조류의 수확 및 미세조류가 생산한 유용 물질 및 지질의 추출 방법을 개시하며, 상기 제2 단계 동안, 상기 초박형 자성 나노입자가 상기 미세조류의 세포벽을 기계적으로 파쇄하여 세포벽 내에 있는 미세조류가 생산한 유용 물질 및 지질을 추출하는 것을 특징으로 한다.

METHOD FOR CULTIVATING CYANOBACTERIA

Resumen de: US2025188443A1

A method for cultivating cyanobacteria includes: providing the cyanobacteria; subjecting the cyanobacteria to physical mutagenesis, so as to obtain primary mutant cyanobacteria; subjecting the primary mutant cyanobacteria to chemical mutagenesis, so as to obtain secondary mutant cyanobacteria; and subjecting the secondary mutant cyanobacteria to a temperature resistance test and an environment resistance test, so as to obtain target cyanobacteria. A fatality rate of the physical mutagenesis ranges between 50% and 80%, and a fatality rate of the chemical mutagenesis ranges between 40% and 55%.

IMPROVEMENT OF NUTRITIONAL VALUE OF FISH FEED

Resumen de: EP4566457A1

This invention relates to optimization of standard fish feed by incorporating a c-phycocyanin additive into a standard fish feed in an oil suspension. Isolated c-phycocyanin, which is isolated from the biomass of the genus Microcystis (microalgae) and Aphanizomenonflos-aquae (Klamath algae), improves the physiological parameters, growth intensity and physical/chemical meat properties of rainbow trout (Oncorhynchus mykiss) and African catfish (Clarias gariepinus).

Method for treating acidic organic wastewater by using mixed algae and application thereof

Resumen de: CN120117754A

The invention discloses a method for treating acidic organic wastewater by using mixed algae. The method comprises the following steps: (1) inoculating and culturing filamentous algae in the acidic organic wastewater; (2) culturing the filamentous algae in the acidic organic wastewater for 6 days, and then adding streptozotoci or/and Widdersonia with the same algae cell concentration to carry out cooperative treatment or gradient treatment on the acidic organic wastewater; (3) fishing algal biomass in the wastewater after the co-treatment or gradient treatment is performed for 6 days; and (4) detecting parameters of the acidic organic wastewater, and judging whether cooperative treatment or cascade treatment needs to be carried out again according to the detected parameters. The obtained algae biomass is used as feed. The method has the innovation points that filamentous algae and high-stain-resistance streptazone algae or/and high-nutrient-value widmannella are subjected to mixed culture in the acidic organic wastewater by adopting a synergistic treatment or gradient treatment mode, so that high-yield algae biomass is obtained while the acidic organic wastewater is treated; an efficient, stable, economical and feasible microalgae wastewater treatment system is realized.

Photobioreactor

Resumen de: CN120098752A

The photobioreactor comprises a box body, a light source assembly and an aeration module, a containing cavity is formed in the box body, the light source assembly is detachably arranged in the containing cavity, a light source is arranged in an outer cylinder, the outer wall of the outer cylinder gradually inclines towards the box body from bottom to top, and an aeration pipe is located below the light source assembly. When the aeration pipe performs upward aeration, as the outer wall of the outer cylinder is in an inclined state, bubbles always rise along the outer wall surface of the box body, so that the bubbles strongly scour the outer wall of the outer cylinder in the rising process to prevent microalgae from growing on the outer wall of the outer cylinder in a wall-attached manner, and light generated by the light source assembly can penetrate through the outer cylinder to be emitted out. The light source assembly is arranged in the reactor, so that the effectiveness of illumination during long-term microalgae culture in the reactor is ensured, the light availability is improved, the microalgae can grow under sufficient illumination, the yield of the microalgae is improved, the illumination intensity of the light source assembly does not need to be improved, and the culture cost of the microalgae is reduced.

Agricultural non-point source pollution control system and method based on nitrogen-fixing cyanobacteria

Resumen de: CN120097757A

Agricultural non-point source pollution is caused by runoff of fertilizers and pesticides in farmland, and especially the problem of water eutrophication caused by nitrogen and phosphorus pollution is serious. Traditional pollution control measures such as reduction of chemical fertilizer consumption and construction of physical isolation belts are limited in effect and high in cost. Nitrogen-fixing cyanobacteria can fix nitrogen in the atmosphere and absorb nitrogen in water, so that the nitrogen-fixing cyanobacteria is considered to be a potential natural agricultural pollution control tool. The invention aims to provide an agricultural non-point source pollution control system based on nitrogen-fixing cyanobacteria and an application method thereof, so as to efficiently replace part of chemical fertilizers, reduce agricultural non-point source pollution, improve soil fertility and promote healthy growth of crops.

Pollution and carbon reduction method for rare earth ammonia nitrogen wastewater by utilizing microalgae closed reaction

Resumen de: CN120097520A

The invention discloses a method for reducing pollution and carbon in rare earth ammonia-nitrogen wastewater by utilizing a microalgae closed reaction. The method comprises the following steps: preparing an ammonia-nitrogen wastewater culture medium, inoculating microalgae, pressurizing CO2 in a reactor, regulating and controlling culture and monitoring and harvesting. According to the invention, the waste gas CO2 discharged in the rare earth production process can be effectively utilized, a cheap carbon source is provided for an ammonia nitrogen biological removal system, meanwhile, carbon reduction and decontamination are realized, and the defect that in the current technical scheme, the biological removal of ammonia nitrogen needs to additionally provide commercial carbon nutrition for the system is overcome; the reactor runs under the micro-positive pressure condition, CO2 gas mass transfer is enhanced, a sufficient carbon source is provided for microalgae growth, system escape of ammonia nitrogen and CO2 is avoided, and the defects that in the current technical scheme, the ammonia nitrogen recycling effect is poor, and ammonia nitrogen escapes are overcome.

Modified corncob as well as preparation method and application thereof

Resumen de: CN120097524A

The invention belongs to the technical field of environmental restoration and biology, and discloses a modified corncob as well as a preparation method and application thereof. According to the method, a mixed solution of NaOH and acetyl bromide is used for modifying corncobs, lignin in the corncobs is removed through alkalization pretreatment in the modification process, the porosity and the specific surface area of the corncobs are increased, and meanwhile, acetyl bromide is adopted for treatment, so that the adsorption capacity and the immobilization efficiency of the corncobs to microalgae are enhanced. The modified corncob can be crosslinked with an exogenous polymer (EPS) secreted by the microalgae, so that the microalgae can be stably attached and survive for a long time, and the application effect of the corncob in wastewater treatment and ecological restoration is improved. The invention provides an efficient and sustainable biological immobilization technology, provides a new technical path for water pollution treatment and ecological restoration, and has a wide application prospect.

Device for treating aquaculture tail water by utilizing marine microalgae and treatment method thereof

Resumen de: CN120097592A

The invention provides a device for treating aquaculture tail water by utilizing marine microalgae and a treatment method thereof, and belongs to the technical field of aquaculture tail water. The device comprises a box body, a pretreatment mechanism and a purification mechanism, a pretreatment bin, a waste collection bin, a purification bin, a first precipitation bin, a second precipitation bin and a third precipitation bin are arranged in the box body, the pretreatment mechanism is installed in the pretreatment bin, and the pretreatment mechanism comprises a filter cartridge and a first rotating shaft; the filter cartridge is rotatably mounted in the pretreatment bin, and a gear ring is fixedly mounted on the surface of the filter cartridge. By arranging the pretreatment mechanism, tail water is introduced into the filter cartridge, suspended matters and organic matters in the tail water are filtered, the subsequent purification efficiency is improved, the filter cartridge is driven by the motor to rotate, impurities accumulated on the inner wall of the filter cartridge are fed into the waste collection bin through a spiral blade to be collected, use is convenient, water is fed into a branch pipe through a water pump, and the water treatment efficiency is improved. The surface of the filter cartridge is spray-washed by the nozzle, so that the filter cartridge is prevented from being blocked by impurities, and the filtering effect is ensured.

Vegetable-Based Food Product

Resumen de: US2025176603A1

A vegetable-based food product for use in chronic autoimmune thyroiditis (Hashimoto's disease) is described. It includes a mixture of cooked or steamed vegetables, nuts, herbs and water. It contains steamed broccoli and Brazil nuts and olive oil with omega-3 hemp oil and thyme and parsley and onion and black mustard and black cumin and garlic and curcumin and/or piperine and beta-glucans as a source of selenium from Shitake mushrooms (Lentinula edodes) along with a liquid extract of microalgae containing omega-3 acids and MCT (oil powder) and inulin and is enriched with a functional additive in the form of a highly concentrated vegetable protein isolate.

CULTURE DEVICE

Resumen de: US2025179407A1

A culture device comprises an accommodation part which is capable of accommodating a culture solution and a microalgae, and a plurality of guide parts which are provided to the accommodation part. A guide part has a main body part and a ceiling part which is provided to an upper end of the main body part. In the main body part, a gas is supplied to a lower end which faces a bottom part of the accommodation part. The ceiling part directs the gas from the guide part in one direction in the width direction of the accommodation part.

GLASS NOODLES WASTEWATER TREATMENT PROCESS BASED ON MICROALGAE

Nº publicación: WO2025112477A1 05/06/2025

Solicitante:

SHANDONG ACAD OF AGRICULTURAL SCIENCES [CN]
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Resumen de: WO2025112477A1

The present invention relates to a glass noodles wastewater treatment process based on microalgae, belonging to the technical field of microbial wastewater treatment. Chlorella sorokiniana NZ1 and Monoraphidium sp. NZ4 are selected. An analysis of experiment results shows that when Chlorella sorokiniana NZ1 and Monoraphidium sp. NZ4 are subjected to mixed culturing, the biomass of both in the wastewater reaches the highest level. At the same time, the removal rates of water quality indicators also exhibit the most significant improvement; specifically, the removal rates of COD, TN, ammonia nitrogen, and TP can respectively reach 83.4%, 91.19%, 81.51%, and 78.71%. Thus, it can be concluded that subjecting the algal strains NZ1 and NZ4 to mixed culturing is the most effective glass noodles wastewater treatment method.