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92
resultados
Última actualización
18/08/2026 [07:49:00]
Resumen de: US20260224161A1
0000 A biosensor for real-time monitoring of a wound can provide valuable data regarding the presence of bacteria. A nanofibrous biosensor is provided for monitoring wound status at the point of care. The colorimetric biosensor changes color in response to low levels of bacteria and fungi, making it visible to unaided and untrained eyes. A colorimetric probe is a hemicyanine dye that changes color from yellow to green in the presence of lipase. Dye is incorporated into a shell composition of core-shell nanofibers made of polyurethane and polyvinylpyrrolidone. As a means of increasing the biosensor's sensitivity, the alignment of nanofibers is controlled and a surfactant added to the shell (e.g., Tween 80). Alignment of nanofiber enables better localization, and Tween 80 increases lipase activity, which facilitates near immediate color changes above critical levels of Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Candida aureus. All ESKAPEE bacteria are detected within 2 hours.
Resumen de: US20260218268A1
Disclosed are nucleic acid oligomers, including amplification oligomers and detection probes, for detection of Yersinia enterocolitica, Vibrio spp., Shiga Toxin Expressing E. coli (STEC) O157, and Plesiomonas shigelloides target nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding formulations, reaction mixtures, and kits. Methods of synthesizing the nucleic acid oligomers are also disclosed.
Resumen de: EP4772522A2
Disclosed herein are methods and compositions for detecting one or more pathogens that cause atypical pneumonia. Detectable pathogens include Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila.
Resumen de: CN122303462A
The invention provides a primer/probe composition for simultaneously detecting shigella and salmonella, a kit and application, and belongs to the technical field of gene detection. The invention comprises a primer/probe set for detecting shigella and a primer/probe set for detecting salmonella. The invention has the advantages of high detection sensitivity, strong specificity, high dual synchronous detection efficiency, good repeatability, good stability and low pollution risk, and is especially suitable for low-load sample detection and rapid screening of monkey group samples.
Resumen de: CN122303454A
The invention discloses a detection method and application of F17 escherichia coli, fimbriae virulence gene ctg04543 or ctg04549 of the F17 escherichia coli is taken as a target, a fluorescent quantitative probe is designed according to a nucleotide sequence, genome DNA of the F17 escherichia coli is taken as a template, the fimbriae virulence gene ctg04543 or ctg04549 of the F17 escherichia coli is detected through fluorescent quantitative PCR, and the F17 escherichia coli in a sample can be quantitatively detected according to a fitted standard curve. The F17 Escherichia coli detection method provided by the invention has the characteristics of simplicity, rapidness, quantitation, high sensitivity and high specificity, can realize quantitative detection of F17 Escherichia coli within 2-3 hours, can meet the detection requirements under various conditions of laboratory research, animal pathogen diagnosis and the like, and has wide application prospects and extremely high market implementation possibility.
Resumen de: CN122278469A
The invention discloses a high-sensitivity rapid fluorescent probe free of metal toxicity, a preparation method and application of the high-sensitivity rapid fluorescent probe in food pathogenic bacterium detection, and belongs to the technical field of food detection. The probe is composed of an organic fluorescent molecule graphene composite main body, an organic-inorganic hybrid dopant and a specific recognition molecule, the doping agent is carboxylated cellulose and silicon dioxide nanoparticles, and the mass ratio of the doping agent to the composite main body is 1: (6-10); the specific recognition molecule adopts a pathogenic bacterium aptamer and is grafted on the surface of the probe through spray coating. The probe triggers fluorescence response by means of specific binding of the aptamer and target pathogenic bacteria, detection can be completed within 5 min, the sensitivity is larger than or equal to 90%, and the specificity is larger than or equal to 98%; the probe does not contain metal components, is free of poison and environmental risks, and is suitable for on-site rapid detection of food and living detection of livestock and poultry; meanwhile, the preparation cost is reduced by 90%, the single sample detection cost is reduced by 40%, and the application prospect is good.
Resumen de: CN122283128A
The invention relates to a method for detecting pathogenic bacteria based on magnetic coding and a microfluidic separation system, and belongs to the technical field of microbiological analysis and detection. The core of the spatial separation system based on magnetic coding comprises a single-channel micro-fluidic chip, a 3D printing magnetic separation module, a fluorescence-ultraviolet dual-mode detection light source and a fluorescence-ultraviolet dual-mode probe. According to the micro-fluidic device, fluid dynamic behaviors and magnetic field gradient distribution in the channel can be accurately regulated and controlled, so that the magnetic nanospheres are efficiently controlled and separated, and the accuracy of multi-target analysis is further enhanced; meanwhile, due to directional enrichment of the magnetically labeled particles in the microchannel, a tedious sample concentration step can be directly omitted, the total analysis time is remarkably shortened, and after an optical or electrochemical sensor is combined, rapid real-time detection of target molecules can be achieved.
Resumen de: CN122282714A
The invention discloses an embedded whole-cell biosensor, a preparation method of the embedded whole-cell biosensor and application of the embedded whole-cell biosensor in machine learning assisted mercury ion detection, and belongs to the technical field of sensor materials and detection. According to the sensor, escherichia coli (E. coli) for expressing green fluorescent protein (GFP) is encapsulated in a polyacrylamide/hyaluronic acid (PAAM/HA) hydrogel matrix, so that the sensor can realize high-selectivity and high-sensitivity detection on mercury ions, can effectively eliminate interference of competitive metal ions, can realize complete regeneration through EDTA-2Na treatment, and can be used for detecting mercury ions in a high-sensitivity manner. The device can be repeatedly used under the condition that the performance is not lost; stable fluorescence response as long as three weeks can be maintained; excellent biocompatibility and biodegradability are achieved, and the risk of secondary pollution is reduced to the maximum extent; and rapid on-site analysis is facilitated.
Resumen de: CN122278855A
The invention discloses sgRNA (small guide RNA), an RPA (recombinase polymerase amplification) primer pair and application of the sgRNA and the RPA primer pair in campylobacter coli detection. The sgRNA contains a target sequence of which the nucleotide sequence is as shown in SEQ ID NO.1 or SEQ ID NO.2. The lowest detection limit of the detection method is 100 CFU/mL, the specificity is high, the detection operation is simple, the detection method is not limited by site equipment and professional ability of operators, the application range is wide, the detection method can be used for rapidly detecting campylobacter colons in actual excrement samples, and the detection result is accurate.
Resumen de: CN122279066A
The invention relates to a method for trace enrichment and detection of Listeria monocytogenes in agricultural products, which comprises the following steps: homogenizing an agricultural product sample to be detected and removing inhibitory impurities to obtain a sample solution; carrying out induction treatment on the sample liquid to expose a recognition site of the listeria monocytogenes; contacting the sample liquid subjected to the induction treatment with a solid-phase carrier with listeria monocytogenes recognition molecules fixed on the surface, so that the target bacteria are selectively locked on the surface of the solid-phase carrier, and washing to remove unlocked substances; performing core release treatment on the target bacteria locked on the surface of the solid-phase carrier to obtain a nucleic acid template; performing specific nucleic acid detection on the nucleic acid template for the listeria monocytogenes, and judging whether the listeria monocytogenes exists in the sample to be detected or not according to a detection result. The method can reduce the adverse effect of a complex agricultural product matrix on subsequent recognition and nucleic acid detection, and improve the sensitivity and accuracy of trace target bacterium detection in a complex sample.
Resumen de: CN122256540A
The invention provides a molecular diagnosis method combining a multi-enzyme isothermal rapid amplification (MIRA) technology and a CRISPR-Cas12a nucleic acid detection technology, and particularly relates to application of the molecular diagnosis method in rapid, sensitive and specific detection of common pathogenic bacteria infected by a central nervous system (CNS). By combining a multi-enzyme isothermal rapid amplification technology with a CRISPR-Cas12a nucleic acid detection system, rapid, sensitive and specific detection of nucleic acid of common pathogenic bacteria of the central nervous system is realized under a constant temperature condition, so that a new technical means is provided for early diagnosis of central nervous system infection.
Resumen de: CN122256541A
The invention provides a kit for detecting common pathogenic bacteria of otitis media based on a multi-enzyme constant-temperature rapid nucleic acid amplification technology. The kit comprises a first primer pair for detecting streptococcus pneumoniae, a second primer pair for detecting haemophilus influenzae, a third primer pair for detecting moraxella catarrhalis, a fluorescent probe combination and a colloidal gold probe combination, the nucleotide sequences of the first primer pair are respectively SEQ ID NO: 2 and SEQ ID NO: 4, the nucleotide sequences of the second primer pair are respectively SEQ ID NO: 9 and SEQ ID NO: 10, and the nucleotide sequences of the third primer pair are respectively SEQ ID NO: 13 and SEQ ID NO: 18. According to the triple multienzyme constant-temperature rapid synchronous detection kit and the detection method, high sensitivity, high specificity and operation convenience are achieved, and the clinical bedside rapid diagnosis requirement is met.
Resumen de: CN122235342A
The invention relates to the technical field of biological detection, and particularly discloses a gene chip for simultaneously detecting 12 food-borne pathogenic bacteria and application thereof, the gene chip comprises a polyvinyl chloride solid-phase carrier, and a specific probe, a positive control probe and a hybridization control probe which are fixed on the surface of the polyvinyl chloride solid-phase carrier. The gene chip can be used for simultaneously detecting salmonella, escherichia coli O157: H7, staphylococcus aureus, listeria monocytogenes, bacillus cereus, vibrio parahaemolyticus, vibrio cholerae, vibrio fluvialis, shigella flexneri, campylobacter jejuni, clostridium perfringens and yersinia enterocolitica. The gene chip disclosed by the invention is arranged by adopting a preset dot matrix, and detection is realized through sample total DNA extraction, multiple PCR amplification and biotin labeling, chip hybridization, washing, sealing, enzyme labeling, color development and scanning interpretation; the method can realize synchronous detection of 12 target bacteria, and has good specificity, repeatability and application value.
Resumen de: US20260168026A1
The present invention relates to a CRISPR-Cas-based composition for detection of Listeria monocytogenes and a Listeria monocytogenes detection method using same and, more specifically, to a composition for detection of Listeria monocytogenes, comprising a primer pair capable of specifically amplifying Listeria monocytogenes by isothermal amplification, a guide RNA, and a CRISPR-Cas protein, and a detection method using same.
Resumen de: CN122214517A
The invention relates to a triple multiple fluorescence detection kit based on a synergistic pathogenic mechanism of enteropathogenic bacteria and application of the triple multiple fluorescence detection kit. The kit comprises a sample treatment solution, targeted capture magnetic beads, an eluent, a multiple PCR premixed solution, a primer probe mixed solution, a positive reference substance, a negative reference substance and an exogenous internal standard, the sequences of the primer probes are as shown in SEQ ID NO. 1-9. The invention also provides a method for detecting enterotoxin-producing bacteroides fragilis, escherichia coli carrying pks gene island and fusobacterium nucleatum in excrement by using the kit, and a method for assisting in evaluating the risk of colorectal cancer. The triple multiple fluorescence detection kit breaks through the technical bottleneck of synchronous and accurate detection of high and low abundance targets in multiple fluorescence PCR, ensures the detection sensitivity of low abundance key targets BFT gene and clbB gene, significantly reduces the false negative risk, and provides a more comprehensive molecular basis for early warning and risk stratification.
Resumen de: CN122211684A
The invention belongs to the technical field of food safety detection, and discloses a self-locking kit for nucleic acid detection of food-borne escherichia coli O157 and an operation method of the self-locking kit. The self-locking kit comprises a self-locking unit consisting of a clamping arm, a guide rail, an elastic element and a lock catch, when the reagent tube is not put in, the lock catch limits the clamping arm, and when the reagent tube is put in, the guide rail and the elastic element are matched with the clamping arm, so that when the buckle end of the lock catch is separated from the limiting groove, the elastic element extends to push the lower end of the clamping arm to slide outwards, and the clamping arm is clamped in the limiting groove. And the upper ends of the clamping arms are driven to fold inwards to clamp the reagent tube. The self-locking unit utilizes a lever linkage principle and a spring energy storage release mechanism to solve the contradiction between fixing and taking and placing convenience of the reagent tube.
Resumen de: CN122214285A
The invention discloses application of a salmonella bacteriophage vBSalDSP13 and a rapid detection method of the salmonella bacteriophage vBSalDSP13. The preservation number of the bacteriophage vBSalDSP13 is GDMCC No: 67876-B1, and the preservation number of the bacteriophage vBSalDSP13 The virulent bacteriophage vBSalDSP13 provided by the invention is wide in host spectrum, can split up to 25 salmonella serotypes, can split two strains of escherichia coli at the same time, and has a characteristic molecular target. The bacteriophage SP13 has good thermal stability, pH stability and chloroform tolerance, is not easy to inactivate in the application process, and has a good inhibition effect on target bacteria in milk and chicken. In conclusion, the bacteriophage SP13 can be used as a single inhibitor or combined with other pathogenic bacteria bacteriophages to form an inhibitor for preventing and controlling pathogenic bacteria such as salmonella and the like.
Resumen de: CN122214522A
The invention discloses an LAMP-CRISPR primer composition, a kit and a detection method for detecting Escherichia coli, and belongs to the technical field of molecular detection. According to the invention, LAMP primers and a Cas12b-sgRNA specific recognition sequence are synergistically designed, an LAMP primer group with nucleotide sequences as shown in SEQ ID NO.1-6 and sgRNA as shown in SEQ ID NO.9 are provided, an LAMP-CRISPR primer composition is formed, and the LAMP-CRISPR primer composition and a CRISPR/Cas12b system are made into the kit. LAMP amplification and CRISPR/Cas12b are combined for use, a one-step constant-temperature reaction system is constructed, the amplification detection process is remarkably shortened, meanwhile, high specificity and high sensitivity are achieved, and the problems that a sepsis pathogenic bacterium detection technology is long in time consumption, poor in specificity, incapable of achieving multiple detection, difficult to apply beside a bed and the like are effectively solved.
Resumen de: CN122189209A
The invention relates to the fields of molecular diagnosis, synthetic biology and in-vitro detection, in particular to a molecular sensor system for directly detecting mycobacterium tuberculosis, which comprises a sequence-specific nucleic acid control module, a robust cell-free transcription and translation system and a detection substrate, the sequence-specific nucleic acid control module comprises a single-chain trigger recognition region designed for target RNA (Ribonucleic Acid) highly expressed by mycobacterium tuberculosis in a sputum microenvironment, and a signal report module regulated and controlled by the trigger recognition region; wherein the signal reporting module is used for coding NanoLuc luciferase; the robust cell-free transcription and translation system comprises a T7 RNA polymerase, an escherichia coli ribosome component, an RNase inhibitor with an effective concentration, and at least one matrix stable factor selected from trehalose and a nonionic surfactant. The detection substrate comprises a coelenterazine derivative which is suitable for the NanoLuc luciferase; therefore, the direct detection of the mycobacterium tuberculosis RNA in the sputum sample is realized.
Resumen de: CN122187691A
The invention relates to a D-alanine biological orthogonal probe platform, discloses a preparation method and application thereof, and belongs to the technical field of biological medicine. The probe A1 is firstly metabolized and doped into cell walls, the cell wall permeability of B1, B2 and C1 is gradually enhanced along with the gradual reduction of molecular weight, the fluorescent probe C1 and A1 are effectively subjected to site-click reaction so as to start fluorescence, and gram-positive bacteria staphylococcus aureus, bacillus subtilis and gram-negative bacteria escherichia coli are specifically detected. L-type bacteria losing cell walls are detected through induction of L-type transformation, and the L-type bacteria are obviously distinguished from normal bacteria containing the cell walls. The probe is also used for researching the anti-microbial drug resistance, and powerful evidence of the conclusion that the L-type bacteria cause the drug resistance of the bacteria is obtained. The research lays a foundation for developing the D-alanine biological orthogonal probe for detecting L-type bacteria and researching and diagnosing clinical pathogenic bacteria.
Resumen de: CN122189228A
The invention belongs to the technical field of molecular detection of plant diseases, and discloses a primer probe composition, a kit and an RPA-LFD detection method for detecting pathogenic bacteria of northern corn leaf blight, and the primer probe composition comprises an upstream primer as shown in SEQ ID NO.1, a downstream primer marked by 5 '-terminal biotin as shown in SEQ ID NO.2, and a primer probe. The probe is marked by a 5'end fluorophore as shown in SEQ ID NO.3, contains a THF site in the middle and is modified by a 3 'end blocking group. The invention also provides a kit containing the composition and an LFD test strip. The detection method comprises the following steps: extracting DNA of a sample, carrying out RPA amplification by adopting the primer probe composition, detecting an amplification product through an LFD test strip, and judging whether the amplification product contains target pathogenic bacteria or not according to a color development result. The method is high in specificity, high in sensitivity, simple, convenient and rapid to operate, visible in result by naked eyes, free of precise instruments and suitable for on-site rapid detection and early diagnosis of northern corn leaf blight pathogenic bacteria.
Resumen de: CN122189216A
The invention discloses a multiple fluorescent quantitative PCR (Polymerase Chain Reaction) detection method and a multiple fluorescent quantitative PCR detection kit capable of simultaneously detecting three microorganisms. The primer pair and probe combination comprises a specific primer pair and a specific probe aiming at escherichia coli, a specific primer pair and a specific probe aiming at pasteurella multocida, and a specific primer pair and a specific probe aiming at clostridium perfringens. According to the method, escherichia coli, pasteurella multocida and clostridium perfringens can be synchronously detected through a single tube, the detection period is greatly shortened, and the detection cost is reduced; the primer probe has excellent compatibility, is simple and convenient to operate, and is suitable for clinical diagnosis and treatment, epidemiological investigation and other multi-scene application.
Resumen de: CN122189217A
The invention relates to a composition of universal oligonucleotide primers and probes for detecting pseudomonas bacteria based on an ERA fluorescence method or an ERA test strip method and a detection method using the composition. The detection method disclosed by the invention can be used for rapidly, specifically and sensitively detecting whether the pseudomonas bacteria exist in the sample or not, is simple and convenient to operate and short in reaction time, does not need complex temperature control equipment, and is suitable for rapid screening, pollution monitoring and risk early warning of the pseudomonas bacteria in milk and dairy product samples, food samples, environmental samples, water samples and related monitoring samples.
Resumen de: CN122189218A
The invention belongs to the technical field of biological diagnosis and environmental detection, and particularly relates to an Escherichia coli detection system and kit based on RPA-Cas12a and application of the Escherichia coli detection system and kit. According to the detection system, a UidA gene is taken as a target sequence, a primer for RPA isothermal amplification is designed, and an RPA amplification product is visually displayed or detected by a microplate reader through an RPA-Cas12a technology, so that whether a sample to be detected contains escherichia coli or not is judged. Compared with a traditional culture detection method, the detection method disclosed by the invention has the advantages that the detection efficiency is remarkably improved, complicated equipment is not needed, and the method is suitable for on-site rapid detection; through real-time fluorescence signal monitoring, a detection result can be obtained in real time, and the operation convenience and efficiency are further improved; besides, the detection method can detect escherichia coli as low as 100 copies/L, the sensitivity is high, the detection period is short, and detection can be completed within 30 min.
Nº publicación: CN122193573A 12/06/2026
Solicitante:
ZHEJIANG UNIV OF TECHNOLOGY
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Resumen de: CN122193573A
The invention discloses a kit for simultaneously detecting two food-borne pathogenic bacteria based on an SERS (Surface Enhanced Raman Scattering) technology as well as a preparation method and application of the kit, synchronous detection of double target bacteria is realized, staphylococcus aureus and escherichia coli O157: H7 can be simultaneously recognized and quantified in single detection through an SERS probe modified by two specific aptamers, and the detection efficiency is remarkably improved. The etSi (at) ZnO-Ag-Au composite substrate constructed by the method disclosed by the invention is strong in anti-interference capability and good in reproducibility. The method is high in sensitivity and low in detection limit. The sample pretreatment is simple, the detection steps are convenient and fast, the portable Raman spectrometer is combined, the detection can be quickly completed on the drug production and storage and transportation site, and the requirement on the professional skill of an operator is low.
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