Alerta

Rapid separation and detection method for pathogenic bacteria in aquaculture water

Resumen de: CN121294686A

The invention discloses a rapid separation and detection method for pathogenic bacteria in a culture water body, and belongs to the field of microbiological detection. According to the method, the sample size is reduced by enriching microorganisms, no complex equipment is needed, and the detection period is remarkably shortened through room-temperature pyrolysis and isothermal amplification LAMP detection, so that the requirements of aquaculture early warning and medication guidance are met; the mutant Bst-WK30 DNA polymerase containing seven specific amino acid mutation sites is adopted for LAMP detection, the amplification efficiency is high, the specificity is high, pathogenic bacteria can be accurately identified, and multiple common pathogenic microorganisms with large harm can be detected. All the components of the kit cooperate to realize efficient splitting decomposition and specific amplification; through cooperation of physical filtration and electrochemical enrichment, the separation device rapidly separates target microorganisms, reduces impurity interference, enriches the microorganisms to a filter membrane within 20 min, and is simple in structure, convenient to carry and suitable for rapid on-site sample treatment.

DETECTION OF ENTEROHEMORRHAGIC ESCHERICHIA COLI

Resumen de: WO2026010501A1

The invention relates to methods for detecting the presence or absence of an enterohemorrhagic Escherichia coli (EHEC) in a sample and/or identifying said EHEC. The invention further relates to such methods comprising subjecting the sample or protein therein to mass spectrometry to obtain mass spectrometry data, comparing the obtained mass spectrometry data with reference data for at least one EHEC strain with a specific serotype and optionally one or more non-EHEC strains and detecting the presence or absence of the EHEC.

DETECTION OF ENTEROHEMORRHAGIC ESCHERICHIA COLI

Resumen de: EP4674974A1

The invention relates to methods for detecting the presence or absence of an enterohemorrhagic Escherichia coli (EHEC) in a sample and/or identifying said EHEC. The invention further relates to such methods comprising subjecting the sample or protein therein to mass spectrometry to obtain mass spectrometry data, comparing the obtained mass spectrometry data with reference data for at least one EHEC strain with a specific serotype and optionally one or more non-EHEC strains and detecting the presence or absence of the EHEC.

Primer set for detecting pathogens causing Salmonella Enteritis in pigs and Method for detecting pathogens using the same

Resumen de: KR20260003546A

본 발명은 돼지 살모넬라성 장염 유발 병원균을 검출할 수 있는 프라이머 세트에 관한 것으로, 본 발명에 따른 프라이머 세트를 이용하여 중합효소 연쇄반응을 통해 돼지 살모넬라성 장염 유발 병원균을 신속, 정확하게 검출할 수 있고, 검출한계가 낮아 소량의 돼지 가검물 시료로부터 병원균을 검출하여 감염병을 조기에 진단할 수 있다.

Kit for rapidly and jointly detecting multiple pathogen nucleic acids related to dyspnea pregnancy outcome and application of kit

Resumen de: CN121272078A

The invention belongs to the technical field of in-vitro diagnostic reagents for rapid detection of human pathogenic microorganisms, and particularly relates to a kit for rapid combined detection of multiple pathogen nucleic acids related to a dyspnea pregnancy outcome and application of the kit. The pathogen is selected from one or more of escherichia coli, candida albicans, candida glabrata, group B streptococcus, streptococcus gonorrhoeae, chlamydia trachomatis or ureaplasma urealyticum, and the kit comprises a pathogen and lactobacillus rapid detection micro-fluidic chip. A primer group for detecting the pathogen and the lactic acid bacillus nucleic acid and an array consisting of an amplification control and a blank control are fixed on the micro-fluidic chip. According to the present invention, the microfluidic chip rapid detection technology and the nucleic acid amplification technology are adopted to achieve the purposes of rapid detection and multiple detection so as to rapidly and conveniently determine the poor prognosis risk of the pregnant woman at present, timely take the specific measures, and avoid the occurrence of the poor prognosis.

Method for synchronously and quickly detecting various mycotoxins by competitive immunochromatography based on microbial nano-enzyme tag and application thereof

Resumen de: CN121276046A

The invention discloses a method for synchronously and quickly detecting various mycotoxins by competitive immunochromatography based on a microbial nano-enzyme label and application, and the method can be used for simultaneously and accurately detecting various common mycotoxins, including aflatoxin B1, zearalenone and fumonisins B1, by utilizing the enhanced catalytic effect mediated by the microbial nano-enzyme label. The surface of Escherichia coli is coated with a SiO2 interlayer so as to improve the structural stability and the solution dispersity, and meanwhile, the surface of Escherichia coli is coated with a layer of catalytic shell composed of Au (at) Pt nanoparticles so as to generate excellent colorimetric and catalytic performance. According to the application, ESi-Au-Pt is introduced into a nano enzyme-ICA system, so that the sensitivity and accuracy of on-site analysis of a complex sample are remarkably improved, the limit of detection (LOD) of synchronous detection of target fungaltoxin is reduced to 6.1/1.6/5.4 pg/mL, the detection range spans 4-5 orders of magnitude, in addition, the established method shows excellent accuracy and stability in a complex matrix, and the method can be applied to the field analysis of fungaltoxin. The method shows a huge potential in the field-based real-time monitoring of small-molecule pollutants.

Multiplex PCR (Polymerase Chain Reaction) primer for simultaneously detecting eight respiratory tract pathogenic bacteria and application of multiplex PCR primer

Resumen de: CN121272080A

The invention belongs to the technical field of bacterium detection, and discloses a multiple PCR primer for simultaneously detecting eight respiratory tract pathogenic bacteria and application thereof. On the basis of a Luminex liquid phase chip technology, specific primers are designed aiming at eight common respiratory tract infection pathogenic bacteria including pseudomonas aeruginosa, staphylococcus aureus, streptococcus pneumoniae, klebsiella pneumoniae, acinetobacter baumannii, stenotrophomonas maltophilia, haemophilus influenzae and escherichia coli; the multiple PCR primers which are high in amplification efficiency, good in specificity and suitable for simultaneously detecting the eight respiratory tract pathogenic bacteria are screened out, the multiple PCR system and microsphere hybridization conditions are continuously optimized, the multiple PCR method for the eight respiratory tract pathogenic bacteria is successfully constructed by applying the Luminex xTAG technology, and the multiple PCR method can be applied to rapid detection of multiple pathogenic microorganisms.

C8 esterase activated double-lock chemiluminescent probe, preparation method thereof and application of C8 esterase activated double-lock chemiluminescent probe in preparation of detection kit for detecting salmonella

Resumen de: CN121270430A

The invention relates to the technical field of chemical analysis and detection, and discloses a C8 esterase activated double-lock chemiluminescence probe, a preparation method thereof and application of the C8 esterase activated double-lock chemiluminescence probe in preparation of a salmonella detection kit, the double-lock chemiluminescence probe is DSCP-CBCN, and the structural formula of the double-lock chemiluminescence probe is shown in the specification. The DSCP-CBCN probe is composed of a C8 esterase cleavable substrate, a phenoxy cyclobutane chemiluminophor and a self-eliminating connexon p-hydroxy benzyl alcohol. After the DSCP-CBCN probe and salmonella are incubated, sodium hypochlorite and hydrogen peroxide are added to generate remarkable chemiluminescence, POCT rapid detection of salmonella can be realized by combining a chemiluminescence microplate reader, the bacterial culture time is shortened to 3-7 hours, the limit of detection (LOD) value is as low as 1.9 CFU/mL, and the sensitivity is improved by 1008 times compared with that of a commercial MUCAP probe. The method is suitable for salmonella large-scale screening in the fields of clinical pathogen diagnosis, food safety detection and environmental health monitoring.

Synchronous detection method for klebsiella pneumoniae and escherichia coli based on RPA-CRISPR/Cas12a and application

Resumen de: CN121272079A

The invention relates to the technical field of molecular biology and pathogenic microorganism detection, and discloses a Klebsiella pneumoniae and Escherichia coli synchronous detection method based on RPA-CRISPR/Cas12a and application, and the method comprises the following steps: extracting DNA from a sample, carrying out isothermal amplification by adopting an RPA primer designed aiming at a Klebsiella pneumoniae rcsA gene and an Escherichia coli uriA gene, and carrying out amplification by adopting an RPA primer designed aiming at a Klebsiella pneumoniae RcsA gene and an Escherichia coli uriA gene; a detection system containing Cas12a enzyme, crRNA and a fluorescence report substrate is constructed, and a result is interpreted through a fluorescence or lateral flow chromatography test strip. Detection can be completed within 70 minutes at the constant temperature of 37 DEG C, the sensitivity reaches 5 * 10 copies/mu L, the specificity is high, no cross reaction exists, the kit is suitable for clinical early screening, hospital infection monitoring and on-site POCT application, and an efficient and reliable new tool is provided for pathogen diagnosis.

Method and device for quantitatively detecting pathogenic bacteria and detecting sensitivity of antibacterial agent

Resumen de: CN121249844A

The invention relates to the technical field of biosensing, in particular to a pathogenic bacteria quantitative detection and antibacterial drug sensitivity detection method and device. Based on the relationship between the concentration of gelatin and the time when a to-be-detected solution passes through a detection interval from bottom to top, a pathogenic bacteria quantitative detection and antibacterial drug sensitivity detection method is obtained; according to the quantitative detection method for the pathogenic bacteria, on the basis of ensuring the sensitivity, the detection time is shortened, and staphylococcus aureus as low as 1 CFU/mL can be detected after incubation for 8 hours. The MIC value measured by the antibacterial drug sensitivity detection method is highly consistent with the result of the traditional gold standard method (broth microdilution method), but the required time is greatly shortened (only about 6 hours are needed, and the standard method needs 18-24 hours).

Electrochemical sensing detection system and method based on bacterial nucleotide metabolism

Resumen de: CN121253627A

The invention relates to the technical field of water toxicity detection, in particular to an electrochemical sensing detection system and method based on bacterial nucleotide metabolism. Comprising the following steps: dispersing carboxylated multi-walled carbon nanotubes in N, N-dimethylformamide to prepare a dispersion liquid with a concentration of 0.5 to 1 mg/mL; adding ZnO nanoparticles, and performing ultrasonic treatment to obtain a mixed dispersion liquid; and dispensing the mixture on the surface of a pretreated glassy carbon electrode, and drying the glassy carbon electrode under an infrared lamp to obtain the nano-composite modified electrode. A three-electrode system formed by taking the nano-composite modified electrode as a working electrode, taking saturated calomel as a reference electrode and taking a platinum column as a counter electrode is connected to an electrochemical workstation; a guanine and xanthine mixed oxidation peak current generated by metabolism of Escherichia coli is used as a comprehensive toxicity effect index, and the toxicity of a target system is evaluated by monitoring the change of the mixed oxidation peak current. According to the invention, by utilizing the synergistic interaction of the nano composite material, the sensitive and rapid detection of the comprehensive toxicity of the water body is realized without adding a mediator.

PCR kit for triple PCR detection of escherichia coli, salmonella enteritidis and riemerella anatipestifer and application of PCR kit

Resumen de: CN121249921A

The invention belongs to the technical field of triple PCR detection PCR kits, and particularly relates to a triple PCR detection PCR kit for escherichia coli, salmonella enteritidis and riemerella anatipestifer and application of the triple PCR detection PCR kit. According to the kit, specific primers are designed by taking an escherichia coli alkaline phosphatase gene, a salmonella enteritidis invasion protein A gene and a riemerella anatipestifer helicase gene as target genes, the specific primers comprise a primer pair aiming at the phoA gene, the sequence of an upstream primer phoA-F is 5 '-TACAGGTACTGCGGGCTTATC-3', the sequence of a downstream primer phoA-R is 5 '-CTTACCGGGAATACATCACA-3', and the length of an amplified target fragment is 622bp; according to the primer pair for the invA gene, the sequence of an upstream primer invA-F is 5 '-AAAAGAAGGGTCGTCGTTAG-3', the sequence of a downstream primer invA-R is 5 '-GGAAGGTACTGCCAGAGGTC-3', the length of an amplified target fragment is 801 bp, and according to the primer pair for the dnaB gene, the sequence of an upstream primer dnaB-F is 5 '-GACGCTATATCATAGATTTAAC-3', the sequence of a downstream primer dnaB-R is 5 '-TCCACCGCTATATATTCTAG-3', and the length of the amplified target fragment is 431 bp.

Multiplex PCR (Polymerase Chain Reaction) method for rapidly detecting pathogenic bacteria of tobacco brown spot

Resumen de: CN121227938A

The invention discloses a multiplex PCR (polymerase chain reaction) method for quickly detecting pathogenic bacteria of tobacco brown spot, which is used for synchronously identifying two main pathogenic bacteria causing tobacco brown spot, namely alternaria tenuissima and alternaria alternata, and comprises the following steps: S1, extracting genome DNA (deoxyribonucleic acid): extracting genome DNA of standard strains of alternaria tenuissima and alternaria alternata as a detection template; the multiple PCR system has the beneficial effects that through system verification, it is confirmed that all primers only generate expected amplification products for target pathogenic bacteria, no cross amplification reaction exists between alternaria alternata and alternaria tenuissima, and it is indicated that the multiple PCR system has high interspecific specificity. The established detection method is high in stability, good in repeatability and suitable for standardized operation under different laboratory conditions. Compared with a traditional morphological identification or single gene sequencing method, the multiplex PCR technology has the advantages that the detection period is remarkably shortened, the operation is simple and convenient, time and labor are saved, the cost is low and the like. The method is suitable for rapid identification of standard strains.

Method for synchronously detecting food-borne pathogenic bacteria based on multiple bacteriophage modified nano-enzyme

Resumen de: CN121231460A

The invention discloses a method for synchronously detecting food-borne pathogenic bacteria based on multiple bacteriophage modified nano enzyme, and belongs to the technical field of biology, a phage coated FeCoCe NF + TMB color development system is adopted to react with a solution to be detected, and the food-borne pathogenic bacteria in the solution to be detected are detected through the color development reaction; in the phage (at) FeCoCe NF + TMB color development system, a preparation method of the phage (at) FeCoCe NF comprises the following steps: immobilizing phages EhpYZU20, SalmpYZU54 and SapYZUH5 on a FeCoCe NF nano enzyme, so as to obtain the phage (at) FeCoCe NF. The preparation method of the phage (at) FeCoCe NF comprises the following steps of: immobilizing phages EhpYZU20, SalmpYZU54 and SapYZUH5 on the FeCoCe NF nano enzyme; according to the invention, synchronous visual detection of three food-borne pathogenic bacteria can be realized.

Detection system for rapidly identifying drug resistance phenotypes of pathogenic bacteria based on machine learning in combination with MALDI-TOF MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry)

Resumen de: CN121237227A

The invention relates to the technical field of clinical microbiological detection, in particular to a detection system for rapidly identifying drug resistance phenotypes of pathogenic bacteria based on machine learning in combination with MALDI-TOF MS. According to the system, optimal detection conditions are screened out, clinically separated pathogenic bacteria and antibiotics are subjected to grouping co-incubation, and MALDI-TOF MS detection is carried out through dynamic sampling at multiple time points; preprocessing the obtained mass spectrum data, and calculating to obtain an enhanced dynamic relative growth (RBD-RG) feature vector matrix; a plurality of machine learning models are trained based on the matrix, and an optimal drug resistance phenotype prediction model is screened out through cross validation, so that the drug resistance phenotypes (sensitivity, mediation and drug resistance) of pathogenic bacteria are quickly and accurately judged. The whole detection process can be completed within 2 hours, the detection efficiency is remarkably improved, key technical support is provided for early-stage precise medication of infectious diseases, and improvement of prognosis of patients and restraint of infection spreading are facilitated.

Method for detecting mouse salmonella by using qPCR (quantitative polymerase chain reaction) technology instead of traditional sacrificial sentinel mouse

Resumen de: CN121227909A

The invention discloses a method for detecting mouse salmonella by using a qPCR (quantitative polymerase chain reaction) technology instead of a traditional sacrificial sentinel mouse, and a salmonella specific primer and probe combination comprises a forward primer with a sequence of 5 '-AGCGTACTGGAAAGGGAAAG-3', as shown in SEQ ID NO.1, a reverse primer with a sequence of 5 '-AGCGTACGAAAG-3', as shown in SEQ ID NO.2, a reverse primer with a sequence of 5 '-AGCGTACGAAGGAAAG-3', as shown in SEQ ID NO.3, the sequence of a reverse primer is 5-ATACCGCCAATAAAGTTCACAAAG-3 ', and the sequence of the reverse primer is as shown in SEQ ID NO. 2; the probe sequence of the probe is 5 '-FAM-CGTCACCTTGATAAACTCTATGCA-BHQ1-3', and the probe sequence of the probe is as shown in SEQ ID NO. 3. The method has the beneficial effects that a real-time fluorescent quantitative PCR method is established by utilizing the specific primer and the double-labeled fluorescent probe, so that rapid detection of salmonella pollution in facilities is realized; according to the method based on the environmental sample, use of additional animals is avoided, operation is easy and convenient, the result is accurate, and sensitivity and specificity are both superior to those of a traditional sentinel mouse method.

Intestinal escherichia coli gene detection kit based on silicon-based micro-fluidic chip and detection method of intestinal escherichia coli gene detection kit

Resumen de: CN121227912A

The invention discloses an intestinal escherichia coli gene detection kit based on a silicon-based micro-fluidic chip and a detection method of the intestinal escherichia coli gene detection kit. The kit provided by the invention comprises a rapid hot start DNA polymerase, a PCR reaction mixed solution, a positive reference substance and a negative reference substance, the kit also comprises the following primers which take the intestinal escherichia coli fliC gene as a target gene, are designed by a PCR fluorescent probe method based on a micro-fluidic chip technical platform: an upstream primer F, a downstream primer R and a detection probe P, and the nucleotide sequences of the primers are shown as SEQ ID NO.1-3. According to the invention, the intestinal escherichia coli gene detection kit which is more comprehensive in detection effect, high in specificity, good in sensitivity, low in omission ratio, convenient, simple and easy to operate is provided.

Detection method and application of mouse pathogenic bacteria

Resumen de: CN121227913A

The invention relates to a multiplex fluorescent quantitative PCR (Polymerase Chain Reaction) method for detecting mouse pathogenic bacteria, which can be used for simultaneously detecting four pathogens, namely salmonella, corynebacterium murine, pseudomonas aeruginosa and klebsiella pneumoniae.

Specific primer set of Pseudomonas aeruginosa and method for detecting Pseudomonas aeruginosa using the same

Resumen de: KR20250178279A

본 발명은 서열번호 1의 올리고뉴클레오티드 프라이머 및 서열번호 2의 올리고뉴클레오티드 프라이머를 포함하는, 슈도모나스 에르지노사(Pseudomonas aeruginosa) 검출용 프라이머 세트; 이를 포함하는 조성물; 이를 포함하는 키트; 및 이를 이용한 슈도모나스 에르지노사(Pseudomonas aeruginosa) 검출 방법에 관한 것이다.

Specific primer set of Listeria monocytogenes and method for detecting Listeria monocytogenes using the same

Resumen de: KR20250177874A

본 발명은 서열번호 1의 올리고뉴클레오티드 프라이머 및 서열번호 2의 올리고뉴클레오티드 프라이머를 포함하는, 리스테리아 모노사이토제네스(Listeria monocytogenes) 검출용 프라이머 세트; 이를 포함하는 조성물; 이를 포함하는 키트; 및 이를 이용한 리스테리아 모노사이토제네스(Listeria monocytogenes) 검출 방법에 관한 것이다.

Method for detecting listeria monocytogenes in livestock and poultry meat based on controllable change of peroxidase-like activity

Resumen de: CN121208336A

The invention belongs to the technical field of biological detection, and particularly relates to a method for detecting listeria monocytogenes in livestock and poultry meat based on controllable change of peroxidase-like activity. According to the method, heme, manganese chloride tetrahydrate and sodium hydroxide are taken as substrates, and the nano-enzyme Mn-Hemin with peroxidase-like activity is synthesized through a room-temperature stirring method. The nano-enzyme Mn-Hemin can specifically adsorb and recognize the listeria monocytogenes and regulate and control the peroxidase activity of the listeria monocytogenes, and finally a method for rapidly determining the listeria monocytogenes in a colorimetric mode is established. According to the listeria monocytogenes detection method provided by the invention, the detection time is only 5 minutes. In addition, Mn-Hemin is outstanding in anti-interference performance, various food-borne pathogenic bacteria similar to listeria monocytogenes can be effectively eliminated, the detection limit is 6.56 CFU/mL, the linear range is 1.0-1.0 * 10 < 5 > CFU/mL, and good sensitivity and accuracy are shown.

Biosensor for detecting pseudomonas fluorescens and detection method

Resumen de: CN121208335A

The invention belongs to the technical field of food safety and biosensors, and particularly relates to a biosensor for detecting pseudomonas fluorescens and a detection method. The biosensor for detecting the pseudomonas fluorescens, provided by the invention, comprises a nano click enzyme at bacteriophage recognition probe, a magnetic adsorption platform and a click substrate, and is a fluorescent biosensing system based on a click nano enzyme mediated signal amplification strategy and bacteriophage specific recognition. By combining the high catalytic activity of the recognition probe on the click reaction, the high-specificity signal amplification advantage of the recognition probe on a target detection object and the enrichment amplification advantage of magnetic adsorption, the probe shows excellent detection sensitivity and specificity in pseudomonas fluorescens detection, the detection limit is 1 CFU/mL, the linear range is 102-107 CFU/mL, and the detection sensitivity is high. Therefore, technical support can be provided for ultra-sensitive and high-specificity detection of the pseudomonas fluorescens, and the method is very suitable for on-site rapid screening of raw milk, pasteurized milk, UHT milk and diluted samples of the pasteurized milk, the UHT milk and the diluted samples of the pasteurized milk and the UHT milk.

Method for synchronously and rapidly detecting escherichia coli and multi-drug-resistant genes thereof

Resumen de: CN121204211A

The invention provides a method for synchronously and rapidly detecting Escherichia coli and multiple drug-resistant genes thereof, which comprises the following steps: culturing a sample at night to obtain a bacterial solution to be detected, adding a buffer solution and a metal bath, centrifuging, taking a supernatant, detecting by micro-fluidic, optimizing the extraction process of nucleic acid in the sample, designing an optimal RPA primer probe group, and detecting the multiple drug-resistant genes of the Escherichia coli. A reasonable RPA coupled microfluidic detection system is established, synchronous and rapid detection of the escherichia coli uidA and I-type integrase gene intI1 thereof and drug-resistant genes sul1 and aadA5 is realized, the specificity is high, the sensitivity is high, the reaction time is short, the operation is convenient, and a method support is provided for monitoring and prevention and control of food-borne drug-resistant bacteria.

Primer group for simultaneously detecting four pathogenic microorganisms and application of primer group in preparation of micro-fluidic chip and kit

Resumen de: CN121182993A

The invention discloses a primer group for simultaneously detecting four pathogenic microorganisms and application of the primer group in preparation of a micro-fluidic chip and a kit. The sequences of the primers are as shown in SEQ ID NO: 1-24. The method has the advantages that the microfluidic technology and the isothermal amplification technology are combined, four sample indexes are detected at the same time in one-time detection, and the method has the advantages of being high in throughput, high in specificity, good in experiment repeatability and convenient and rapid to operate; the kit can be used for rapidly and accurately detecting salmonella (SAL), staphylococcus aureus (SA), listeria monocytogenes (LM) and escherichia coli O157: H7 (E. coli O157: H7).

Composition for detecting respiratory tract infection pathogens, kit and application

Nº publicación: CN121182992A 23/12/2025

Solicitante:

SANSURE BIOTECH INC
\u5723\u6E58\u751F\u7269\u79D1\u6280\u80A1\u4EFD\u6709\u9650\u516C\u53F8

Resumen de: CN121182992A

The invention relates to the technical field of biological detection, and discloses a composition for detecting respiratory tract infection pathogens, a kit and application. The composition disclosed by the invention can be used for rapidly and simultaneously detecting legionella pneumophila, bordetella pertussis, chlamydia pneumoniae, mycoplasma pneumoniae, mycobacterium tuberculosis, aspergillus and cryptococcus neoformans in a sample with high specificity, and has relatively high sensitivity and stability. Compared with traditional detection methods such as pathogen isolated culture and genome sequencing, the efficiency of identifying and jointly detecting multiple respiratory tract pathogens by adopting the composition disclosed by the invention is effectively improved, the detection process is simple and convenient to operate, and the composition is suitable for large-scale popularization and application.