Ministerio de Industria, Turismo y Comercio LogoMinisterior
 

Alerta

Resultados 52 resultados
LastUpdate Última actualización 13/10/2026 [07:45:00]
pdfxls
previousPage Resultados 50 a 52 de 52  

LAMP-CRISPR primer composition for detecting Escherichia coli, kit and detection method

NºPublicación:  CN122214522A 16/06/2026
Solicitante: 
BEIHANG UNIV
\u5317\u4EAC\u822A\u7A7A\u822A\u5929\u5927\u5B66
CN_122214522_PA

Resumen de: CN122214522A

The invention discloses an LAMP-CRISPR primer composition, a kit and a detection method for detecting Escherichia coli, and belongs to the technical field of molecular detection. According to the invention, LAMP primers and a Cas12b-sgRNA specific recognition sequence are synergistically designed, an LAMP primer group with nucleotide sequences as shown in SEQ ID NO.1-6 and sgRNA as shown in SEQ ID NO.9 are provided, an LAMP-CRISPR primer composition is formed, and the LAMP-CRISPR primer composition and a CRISPR/Cas12b system are made into the kit. LAMP amplification and CRISPR/Cas12b are combined for use, a one-step constant-temperature reaction system is constructed, the amplification detection process is remarkably shortened, meanwhile, high specificity and high sensitivity are achieved, and the problems that a sepsis pathogenic bacterium detection technology is long in time consumption, poor in specificity, incapable of achieving multiple detection, difficult to apply beside a bed and the like are effectively solved.

Application of salmonella bacteriophage vBSalDSP13 and rapid detection method of salmonella bacteriophage vBSalDSP13

Nº publicación: CN122214285A 16/06/2026

Solicitante:

INST OF MICROBIOLOGY GUANGDONG ACADEMY OF SCIENCES GUANGDONG DETECTION CENTER OF MICROBIOLOGY
GUANGDONG HUANKAI BIOTECHNOLOGY CO LTD
\u5E7F\u4E1C\u7701\u79D1\u5B66\u9662\u5FAE\u751F\u7269\u7814\u7A76\u6240\uFF08\u5E7F\u4E1C\u7701\u5FAE\u751F\u7269\u5206\u6790\u68C0\u6D4B\u4E2D\u5FC3\uFF09
\u5E7F\u4E1C\u73AF\u51EF\u751F\u7269\u79D1\u6280\u6709\u9650\u516C\u53F8

CN_122214285_PA

Resumen de: CN122214285A

The invention discloses application of a salmonella bacteriophage vBSalDSP13 and a rapid detection method of the salmonella bacteriophage vBSalDSP13. The preservation number of the bacteriophage vBSalDSP13 is GDMCC No: 67876-B1, and the preservation number of the bacteriophage vBSalDSP13 The virulent bacteriophage vBSalDSP13 provided by the invention is wide in host spectrum, can split up to 25 salmonella serotypes, can split two strains of escherichia coli at the same time, and has a characteristic molecular target. The bacteriophage SP13 has good thermal stability, pH stability and chloroform tolerance, is not easy to inactivate in the application process, and has a good inhibition effect on target bacteria in milk and chicken. In conclusion, the bacteriophage SP13 can be used as a single inhibitor or combined with other pathogenic bacteria bacteriophages to form an inhibitor for preventing and controlling pathogenic bacteria such as salmonella and the like.

traducir