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71
resultados
Última actualización
27/06/2026 [08:22:00]
Resultados 50 a 71 de 71
Resumen de: CN121718619A
The invention belongs to the technical field of cosmetic detection, and particularly relates to a method, a primer group and a kit for simultaneously detecting six pathogenic bacteria in cosmetics based on a gene membrane chip technology, co-enrichment culture, genome DNA extraction, multiple PCR amplification, gene membrane chip preparation and membrane chip color development detection, and an experimental result is judged according to the actual color development condition of a target. According to the present invention, the color developing points on the membrane chip are compared with the probe layout map, the internal reference color developing shows that the bacteria are detected, the color developing of each target point represents the detection of each specific bacteria, and the detection method has characteristics of simple operation, high accuracy, good repeatability, strong specificity, high stability and high sensitivity.
Resumen de: CN121674527A
The invention relates to a rapid drug sensitivity detection reagent and detection method for pathogenic bacteria of aquatic animals, and the rapid drug sensitivity detection reagent for pathogenic bacteria of aquatic animals comprises the following components in percentage by weight: 1-3% of 2, 3, 5-triphenyl tetrazole chloride, 0.5-1% of iodonitrotetrazole, 5-10% of sodium chloride, 5-8% of sucrose, 0.1-0.5% of vitamin C powder and the balance of water. The invention also discloses a rapid drug sensitivity detection method of the detection reagent for pathogenic bacteria of aquatic animals. The rapid drug sensitivity detection reagent for the pathogenic bacteria of the aquatic animals is short in detection time, suitable for detection of various aquatic pathogenic bacteria, capable of supporting rapid screening of various antibiotics, low in cost and convenient to popularize and use in a large scale. The detection method is simple to operate and short in time consumption, can quickly complete drug sensitivity experiments of pathogenic bacteria of aquatic animals in batches, and is convenient for quickly and accurately screening out drugs for treating and preventing related bacterial diseases of the aquatic animals.
Resumen de: CN121678650A
The invention provides a method for detecting residual blood through object surface disinfection wet tissue, and relates to the technical field of medical environment, medical equipment and equipment surface cleaning detection. After the object surface disinfection wet tissue with the residual blood detection function is used for collecting surface samples of a medical environment, a medical instrument and equipment, the residual blood pollution degree of the surfaces of the medical environment, the medical instrument and the equipment is qualitatively, semi-quantitatively and quantitatively judged according to the color change of the object surface disinfection wet tissue with the residual blood detection function. The semi-quantitative detection calibrator comprises multiple stages of color blocks and subdivision grids, and the residual blood volume is calculated by counting the area proportion of different color blocks through visual inspection; according to the quantitative detection AI rule, wet tissue images are scanned through photography and analysis equipment, software analyzes the mapping relation between pixel RGB values and standard concentration, the method is easy and convenient to operate, large-batch data can be rapidly processed, the cost is remarkably reduced, and the method is particularly suitable for rapid pollution evaluation of medical environments, medical instruments and equipment surfaces. Meanwhile, the pirameton can generate a composite reaction wi
Resumen de: CN121674407A
The invention provides a salmonella lateral flow chromatography biosensor. The invention particularly relates to a sandwich lateral flow chromatography sensor consisting of a nucleic acid nano enzyme and a bacteriostatic aptamer. When a target to be detected exists, the target to be detected is combined with the nucleic acid nano-enzyme probe on the combination pad, flows through the capillary action and is specifically intercepted by the Sal5-4-17nt aptamer when passing through the T line, and the redundant nucleic acid nano-enzyme probe continuously flows to the C line through the capillary action and is intercepted and captured through hybridization among nucleic acid sequences; therefore, after the color developing solution is added, obvious blue strips appear on T and C; when the to-be-detected target does not exist, the nucleic acid nano-enzyme probe is not intercepted by the T line and is intercepted only when the to-be-detected target passes through the C line, that is, only the C line has a blue band after the developing solution is added. The test paper can realize visual detection of salmonella within 5 minutes after probe dosage and hybridization sequence design and color developing solution and color developing time optimization, and meanwhile, the test paper has good biological safety.
Resumen de: CN121678796A
The invention belongs to the field of electrochemical sensors, and discloses an anti-pollution microelectrode for identifying pseudomonas aeruginosa strains and detecting phenazine spatial distribution of the pseudomonas aeruginosa strains as well as a preparation method and application of the anti-pollution microelectrode. The anti-pollution microelectrode comprises a carbon fiber electrode base body, a Fe-HHTP-PDA composite substrate layer and a Fe-HHTP-SBMA anti-pollution layer. The two-dimensional conductive metal organic framework material Fe-HHTP is doped in the anti-pollution layer based on the PDA substrate and the SBMA, so that the problem of signal attenuation caused by insulativity of the anti-pollution layer is successfully solved; by virtue of a two-dimensional ordered structure and a mixed valence iron node, the Fe-HHTP not only serves as a conductive bridge to maintain electron transmission, but also specifically catalyzes an oxidation-reduction reaction of phenazine substances, so that the anti-pollution function is realized, and meanwhile, the detection sensitivity is maintained and even improved. The preparation method is simple and convenient to operate and controllable in modification, and does not need a traditional high-pressure and high-temperature process.
Resumen de: CN121653276A
The invention discloses a fluorescent quantitative PCR (polymerase chain reaction) primer group for detecting pathogenic bacteria of tomato root rot and application, and relates to the technical field of molecular biology and plant disease detection, the primer group comprises a primer pair BT-F/BT-R for specifically detecting the pathogenic bacteria of phytophthora and a primer pair EF-F/EF-R for specifically detecting fusarium oxysporum, the nucleotide sequences of the primers are respectively shown as SEQ ID NO: 1-4. The invention also provides a kit containing the primer group and a method for detecting tomato root rot pathogenic bacteria, and the method comprises the following steps: extracting genome DNA of a sample to be detected, performing SYBR Green I real-time fluorescent quantitative PCR amplification by taking the obtained genome DNA as a template and adopting the primer group, and detecting the tomato root rot pathogenic bacteria according to the fluorescent quantitative PCR amplification result. And judging whether the to-be-detected sample contains phytophthora pathogenic bacteria and fusarium oxysporum or not. According to the invention, tomato root rot pathogens phytophthora and fusarium oxysporum can be specifically detected, and accurate identification and quantitative detection of the phytophthora and fusarium oxysporum can be realized.
Resumen de: US20260071283A1
Disclosed herein are methods for detecting virulent Shiga toxin-producing E. coli (STEC) strains O26, O103, O121, and O111 in a biological sample comprising the steps of: (i) enriching the bacterial concentration of the biological sample to result in an enriched sample; (ii) isolating DNA from said enriched biological sample; and (iii) detecting virulent strain in said isolated DNA sample via real-time PCR and a melt curve assay. Also disclosed are primers for said assay, as well as kits comprising said primers.
Resumen de: CN121633474A
The invention relates to a double-probe detection kit for detecting escherichia coli O157: H7 and a preparation method of the double-probe detection kit, and belongs to the technical field of food safety. The double-probe detection kit consists of a magnetic separation probe reagent and a copper-manganese double-metal nano-enzyme probe reagent which are independently packaged. The magnetic separation probe reagent is a concanavalin agglutinin A modified magnetic bead, and is used for specifically binding escherichia coli O157: H7 in a meat product detection sample and carrying out separation and enrichment treatment; the bimetallic nano-enzyme probe reagent is copper-manganese bimetallic nano-enzyme modified by a nucleic acid aptamer, and is used for specifically combining with the escherichia coli O157: H7 subjected to separation and enrichment treatment and generating an escherichia coli O157: H7 concentration dependent colorimetric signal and a fluorescence signal. According to the invention, mutual verification is carried out between dual-mode signals, so that the accuracy of a detection result is improved; the detection time is within 1.5 h, and the method has good specificity for common type strains. The invention provides a design thought for the development of detection sensors for escherichia coli O157: H7 and other meat-derived pathogenic bacteria.
Resumen de: KR20260032656A
본 발명은, 리스테리아 모노사이토제네스(Listeria monocytogenes) 또는 리스테리아 이노쿠아(Listeriainnocua)의 검출용 프라이머 세트, 이를 포함하는 키트 및 상기 균의 검출 방법에 대한 것이다.
Resumen de: KR20260030992A
본 발명은 살모넬라 검출을 위한 고리매개등온증폭법 기반 측방유동스트립에 관한 것으로, 보다 상세하게는 고리매개등온증폭(loop-mediated isothermal amplification)용 프라이머 세트; 상기 프라이머 세트에 의해 증폭되는 증폭산물에 특이적으로 결합하는 프로브; 및 측방유동스트립(lateral flow strip);을 포함하는, 살모넬라 속 균을 검출하기 위한 측방유동검사(lateral flow assay) 키트 및 상기 키트를 이용한 살모넬라 속 균의 검출방법에 관한 것이다.
Resumen de: CN121609765A
The invention discloses a competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit capable of accurately quantifying feline panleucopenia virus antigen. The kit comprises an elisa plate, cat panleucopenia virus negative/positive serum, a cat panleucopenia virus standard antigen and an elisa secondary antibody. Wherein the elisa plate is a recombinant protein coated with feline panleucopenia virus VP2, and the protein is a protein shown as a sequence 4 in a sequence table. A competitive ELISA method is adopted, the feline panleukopenia virus VP2 protein expressed by an escherichia coli expression system is small in antigen dosage and high in sensitivity and specificity, whether feline panleukopenia virus antigen exists or not can be efficiently detected, and the kit is high in specificity and specificity. Meanwhile, different standard substances can be used for accurately quantifying the content of the feline panleucopenia VP2 protein or the feline panleucopenia virus titer in the antigen or the vaccine. The kit disclosed by the invention is high in sensitivity, good in specificity and convenient to operate, and has a good market prospect.
Resumen de: CN121610589A
The invention belongs to the technical field of microbiological detection, and particularly relates to a rapid detection method of pseudomonas aeruginosa RAA-CRISPR/Cas12a and application of the rapid detection method of pseudomonas aeruginosa RAA-CRISPR/Cas12a. According to the invention, based on the combination of recombinase polymerase amplification (RAA) and a CRISPR-Cas12a (clustered regularly interspaced short palindromic repeats-CRISPR-Cas12a) system, the ultrasensitive and rapid detection of the Pseudomonas aeruginosa is realized through constant-temperature nucleic acid amplification and specific gene recognition. The detection limit of the method for detecting the pseudomonas aeruginosa is 5.37 * 10 < 1 > copies/L (genome DNA) or 6.4 * 10 < 1 > CFU/mL (bacterial liquid); the detection time is only 21 minutes, and the method can be applied to rapid detection of various environmental samples.
Resumen de: CN121610489A
The invention discloses a droplet microfluidic detection system for detecting pathogenic bacteria based on a deoxyribozyme probe, which belongs to the technical field of microbiological detection, and mainly comprises an automatic sample introduction device, a microfluidic droplet generation chip, a capillary tube-laser induced fluorescence detector and a signal acquisition and analysis device, the pathogen detection process comprises the following steps: respectively and automatically injecting an actual water sample, a deoxyribozyme probe, liquid drop oil, a pathogen lysis solution and a reaction buffer solution into the microfluidic liquid drop generation chip by the automatic sample injection device, stably generating liquid drops, and detecting the liquid drops through the capillary tube-laser induced fluorescence detector, and the signal acquisition and analysis device acquires and analyzes the fluorescence signal. The method has the advantages of high detection flux, high stability, high sensitivity, rapidness and the like, and can be applied to the field of environmental microbiological detection.
Resumen de: US20260062763A1
The present disclosure relates primer pairs, probes, kits, and methods of use thereof to detect virulent strains of Shiga toxin-producing Escherichia coli (E. coli) (STEC).
Resumen de: ES3057757A1
The present invention claims a biosensor, the method for obtaining it, and its use for the detection of Pseudomonas aeruginosa in clinical patient samples. This biosensor is based on the implementation of molecular gates on porous organic-inorganic hybrid supports that allow for the specific and selective recognition of bacterial DNA. The developed molecular gate comprises an oligonucleotide sequence complementary to a specific region of the DNA of this bacterium. The external surface of the porous support is chemically modified to provide reactive groups capable of forming a bond with short-sequence oligonucleotides (O1) designed to act as anchors and subsequently bind the specific oligonucleotides for recognizing P. aeruginosa (O2). In the presence of P. aeruginosa genomic DNA, the O2 oligonucleotides are able to specifically recognize it by complementarity and migrate from the pore surface, leading to the release of the fluorescent indicator. (Machine-translation by Google Translate, not legally binding)
Resumen de: CN121592790A
The invention relates to the technical field of biological detection, in particular to a primer probe group for detecting pathogens of milk goat-derived zoonosis and a kit and application thereof. The kit can be used for simultaneously detecting Escherichia coli, clostridium perfringens, cryptosporidium and Giardia lamblia in the same system, and has the advantages of good repeatability, high sensitivity, strong specificity, simplicity in operation and the like compared with the existing kit. The primer probe group contains any one or more specific primers and probes for detecting Escherichia coli, cryptosporidium, Giardia cyanea and clostridium perfringens respectively, and the primer probe group is used for preparing the kit for detecting the milk goat-derived zoonosis pathogen. The invention further discloses a kit for detecting the milk goat-derived zoonosis pathogen and the kit for detecting the milk goat-derived zoonosis pathogen and the kit for detecting the milk goat-derived zoonosis pathogen and the kit for detecting the milk goat-derived zoonosis pathogen.
Resumen de: CN121595864A
The invention discloses a method for detecting campylobacter jejuni cell swelling toxin CdtB in food, and provides application of a campylobacter jejuni cell swelling lethal toxin CdtB monoclonal antibody to detection of campylobacter jejuni in food for the first time. The invention aims to establish the detection kit and the detection method based on the campylobacter jejuni cell swelling toxin CdtB monoclonal antibody, and the detection kit and the detection method have relatively strong specificity and sensitivity. The CdtB monoclonal antibody prepared by the invention can be combined with CdtB protein secreted by campylobacter jejuni in food, so that the detection of the campylobacter jejuni is realized. The detection speed is high, semi-quantitative detection can be achieved, the detection sensitivity is high, and the lowest CdtB mass of 0.01 microgram can be detected.
Resumen de: CN121574809A
According to the intelligent auxiliary system for single-tube detection of the food-borne pathogenic bacteria, the One-pot-RPA-CRISPR/Cas12a reaction system is combined with the embedded artificial intelligence detection device, so that high-sensitivity and high-specificity detection and automatic grading judgment of the food-borne pathogenic bacteria are realized; through wireless interaction between the portable detection equipment and the intelligent terminal, the portability and field applicability of the equipment are realized; by introducing a YOLOv8n model and an OpenCV image processing algorithm, automatic identification and quantitative analysis of fluorescence signals are realized, subjective errors of manual interpretation are avoided, and the detection efficiency and consistency are improved; the system has good expandability, can meet the detection requirements of various pathogens or biomarkers by replacing primers and crRNA, is suitable for various scenes such as food pollution monitoring, primary medical diagnosis, family health management and the like, and has wide social benefits and application prospects.
Resumen de: CN121575133A
The invention belongs to the technical field of biological analysis and detection, and particularly discloses a live salmonella detection system and method based on deoxyribozyme activation, the detection system comprises a Sub probe, a deoxyribozyme probe, a hairpin chain probe, a hyperbranched dendritic nano-molecule, a crRNA/Cas12a binary compound, a fluorescent probe and magnetic beads, the hyperbranched dendritic nano-molecule comprises a substrate A, a substrate B and a trigger chain B probe, the substrate A is composed of an A-F chain probe, an A-Q chain probe and an auxiliary chain A probe, and the substrate B is composed of a B-F chain probe, a B-Q chain probe and an auxiliary chain B probe. According to the detection system, ribonuclease H2 released only by metabolism of living salmonella is specifically recognized, and is utilized to activate a Sub-Dz substrate, so that the subsequent reaction can be triggered only by viable bacteria from the source, the problem of dead bacteria interference is effectively solved, and high-sensitivity and high-specificity rapid detection of the viable salmonella is realized.
Resumen de: CN121575130A
The invention belongs to the technical field of biology, and particularly relates to an LAMP primer group, a kit and a method for detecting pathogens in fresh milk, a visual LAMP detection method of listeria monocytogenes is established by taking a gyrB gene of the listeria monocytogenes as a target, and a visual LAMP detection method is established by taking a mycobacterium tuberculosis Rv2875 gene as a target. The kit has good specificity and good sensitivity, and still has good specificity and sensitivity when being used for detecting artificially polluted cow milk.
Nº publicación: CN121575084A 27/02/2026
Solicitante:
ANHUI MEDICAL UNIV
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Resumen de: CN121575084A
The invention discloses a hot spot self-assembly colorimetric-Raman sensing platform and an escherichia coli detection method, the hot spot self-assembly colorimetric-Raman sensing platform comprises an isothermal amplification system, a CRISPR-dCas9 system, GNPs-probe and SA-GNPs, a target bacterial gene is taken as a DNA template, RPA amplification is carried out through the isothermal amplification system, and a double-chain amplification product modified by terminal biotin is obtained; the CRISPR-dCas9 system is used for targeted recognition of a double-chain amplification product modified by terminal biotin to form a composite product; gNPs-probe is composed of gold nanoparticles, a Raman signal molecule and a DNA probe, and the GNPs-probe is combined with the stem-loop structure of the sgRNA of the composite product through the DNA probe; sA-GNPs is combined with the biotin of the composite product through streptavidin; gNPs-probe and SA-GNPs are assembled on the composite product, so that a colorimetric-Raman sensing platform for detecting target bacteria is formed. According to the present invention, the cross validation of the generated colorimetric, ultraviolet and Raman multi-mode signals is adopted to improve the result reliability, the isothermal amplification and the self-assembly hot spot are adopted to enhance the multiple sensibilization detection signal, and the specificity is enhanced based on the specific primer group and the CRISPR-dCas9 system mediated s
BOPI
Sede Electrónica
