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Holding type listeria monocytogenes specific molecular target and rapid detection method thereof

Resumen de: CN122060880A

The invention discloses a persistent listeria monocytogenes specific molecular target and a rapid detection method thereof, and belongs to the technical field of microbiological detection. The molecular target is obtained through bioinformatics analysis and screening, is further screened through specific experimental verification, and is respectively specific genome sequence fragments of six retention types of listeria monocytogenes CC5, CC8, CC31, CC87, CC121 and CC204. A corresponding specific primer group is designed based on the target, sample DNA is amplified through PCR, a product is subjected to visual analysis in combination with gel electrophoresis, and whether the target retention type strain exists or not can be judged according to the size of a band. The detection method has the advantages of being short in detection time, low in cost, simple and convenient to operate and high in specificity, results are accurate, judgment is easy, practicability is high, the limitation that traditional culture and identification periods are long, and phenotypic analysis steps are tedious is broken through, and the detection method is suitable for rapid screening of the persistent listeria monocytogenes in samples of food, environment and the like.

Multiple qPCR (quantitative polymerase chain reaction) primer group and kit for detecting core pathogenic bacteria in aquaculture and application of multiple qPCR primer group and kit

Resumen de: CN122038618A

The invention discloses multiple qPCR (quantitative polymerase chain reaction) primers and a kit for detecting core pathogenic bacteria in aquaculture and application of the multiple qPCR primers and the kit. The multiple qPCR primer group comprises a primer combination A (SEQ ID NO.1-3) for detecting edwardsiella tarda, a primer combination B (SEQ ID NO.4-6) for detecting streptococcus iniae and a primer combination C (SEQ ID NO.7-9) for detecting aeromonas salmonicida. The invention also provides a kit containing the primer group and a detection method. A single-tube multiple detection system is constructed for three pathogenic bacteria which are most harmful in aquaculture, detection is rapid, and compared with a traditional culture method, the period is greatly shortened; the method is high in flux, low in cost and suitable for large-scale monitoring; and the kit has strong specificity and high sensitivity, and can realize early screening. The invention provides an effective tool for rapid, accurate and high-throughput detection of core pathogenic bacteria in aquaculture.

Convection amplification primer group, probe and kit for multiple detection of lower respiratory pathogens

Resumen de: CN122038621A

The invention belongs to the field of biomedical treatment, and particularly relates to a convection amplification primer group, a probe and a kit for multiple detection of lower respiratory pathogens. The convection amplification primer pair comprises a legionella pneumophila primer pair, an adenovirus primer pair and a chlamydia psittaci primer pair, primer sequences in the legionella pneumophila primer pair, the adenovirus primer pair and the chlamydia psittaci primer pair respectively comprise a target nucleic acid complementary sequence and an interface sequence, and the interface sequence is located at the 5'end of the target nucleic acid sequence; the nucleic acid is not complementary with the target nucleic acid; the target nucleic acid complementary sequence has a folded sequence, and the folded sequence is complementary to the interface sequence, so that the primer sequence has a bent hairpin structure. Meanwhile, a primer probe is designed, and a respiratory tract pathogenic bacteria multiple detection kit is prepared. The multiple detection can avoid non-specific amplification and improve the detection sensitivity.

Method for detecting multiple pathogenic bacteria by using magnetic separation time difference

Resumen de: CN122042964A

The invention relates to a method for detecting multiple pathogenic bacteria by using magnetic separation time difference, and belongs to the technical field of microbiological analysis and detection. According to the invention, the dual-mode aptamer sensor is integrated, so that rapid and accurate multiple pathogenic bacteria detection can be realized. The core of the time separation system based on magnetic coding comprises fluorescent magnetic coding nanoparticles, a gold nanoparticle-aptamer and a fluorescent-ultraviolet dual-mode probe. A preparation process of the fluorescent-ultraviolet dual-mode probe specifically comprises the following steps: sequentially anchoring quantum dots and specific cDNA on the surface of a magnetic coding nanosphere; and carrying out functional combination with aptamer-modified gold nanoparticles to finally synthesize the dual-mode probe with both fluorescence signal response and ultraviolet absorption response. The probe can realize targeted recognition and separation detection of various pathogenic bacteria through specific binding of the surface aptamer and target pathogenic bacteria.

RPA-CRISPR method for rapidly detecting pathogens in air

Resumen de: CN122038544A

The invention belongs to the technical field of environmental biological safety monitoring, and particularly relates to an RPA-CRISPR method for rapidly detecting pathogens in air. According to the invention, a two-step detection strategy integrating RPA isothermal amplification and CRISPR detection is established; the method specifically comprises the following steps: firstly, acquiring genome DNA of an air sample, and carrying out specific amplification on a trace target by utilizing an RPA technology; then, a product is introduced into a CRISPR-Cas12a system, and the trans-shear activity is recognized and activated by utilizing the specificity of crRNA, so that signal cascade amplification is realized. Pseudomonas aeruginosa is used as a model pathogen, and a primer and probe combination is designed aiming at a specific gene of the pseudomonas aeruginosa. Results show that the method can complete detection within 10-15 minutes at the soonest, the lowest detection limit can reach a single copy level (100 copies/L), and the method has the advantages of high environmental interference resistance, high sensitivity and no need of large instruments, and can meet the on-site rapid screening requirements of air pathogen aerosol in public places, medical environments and other scenes.

Primer probe group for simultaneously detecting six pathogenic bacteria, multiple fluorescent quantitative PCR (Polymerase Chain Reaction) method and kit

Resumen de: CN122038605A

The invention relates to a primer probe group, a multiplex fluorescent quantitative PCR method and a kit for simultaneously detecting six pathogenic bacteria, and belongs to the technical field of molecular biology, the primer probe group comprises primer sequences and probe sequences shown in SEQ ID No.1-18 in a table 1; the 5'end of each probe sequence is modified with a reporter group, and the 3 'end of each probe sequence is modified with a quenching group; the reporter group is Cy5, FAM and VIC, and the quenching group is Eclipse. The invention provides a method for simultaneously detecting six pathogenic bacteria including vibrio parahaemolyticus, salmonella, staphylococcus aureus, escherichia coli O157: H7, listeria monocytogenes and shigella in the same reaction system by combining a multiple real-time fluorescent quantitative PCR (Polymerase Chain Reaction) technology. The multiplex real-time fluorescent quantitative PCR kit provided by the invention is used for detecting the six pathogenic bacteria, can improve the detection sensitivity and specificity, greatly improves the detection efficiency, and overcomes the defects of complex steps, long detection period and incapability of adapting to large-scale rapid detection in the conventional detection method.

Biosensor detection reagent for detecting salmonella typhimurium and preparation method

Resumen de: CN122038409A

The invention discloses a biosensor detection reagent for detecting salmonella typhimurium and a preparation method, and belongs to the technical field of biomedicine. According to the invention, a DNA sequence of an amino-modified nucleic acid aptamer for detecting salmonella typhimurium is screened out and is shown as SEQ ID No: 1. Based on the nucleic acid aptamer, amino modified aptamer-fluorescent nano-enzyme serving as an energy donor and sulfydryl modified aptamer-gold nanoparticles serving as an energy receptor are mixed according to the volume ratio of 1: 1, and the biosensor detection reagent is prepared. The linear detection range of the biosensor detection reagent on salmonella typhimurium is 101-107 CFU/mL, the linear correlation coefficient R2 is equal to 0.9981, and the detection limit is as low as 1.527 CFU/mL. The biosensor detection reagent disclosed by the invention still keeps good selectivity and stability in a complex environment with high protein, high fat and high salt, and is suitable for rapid field detection in the fields of food safety and medical diagnosis.

Respiratory pathogen convection amplification primer pair, probe and multiple detection application

Resumen de: CN122038620A

The invention belongs to the field of biomedical treatment, and particularly relates to a convective amplification primer pair and probe for respiratory tract pathogenic bacteria and multiple detection application of the convective amplification primer pair and probe. The convection amplification primer pair comprises a legionella pneumophila primer pair, a mycoplasma pneumoniae primer pair and a bordetella pertussis primer pair, and the legionella pneumophila primer pair, the mycoplasma pneumoniae primer pair and the bordetella pertussis primer pair are respectively composed of an upstream primer and a downstream primer; each of the upstream primer and the downstream primer comprises a target nucleic acid complementary sequence and an interface sequence, and the interface sequence is located at the 5'end of the target nucleic acid complementary sequence and is not complementary with target nucleic acid; the target nucleic acid complementary sequence has a folded sequence, and the folded sequence is complementary with the interface sequence, so that the primer sequence has a curved hairpin structure, a probe is designed, and a respiratory tract pathogenic bacteria multiple detection kit is prepared. The multiple detection can avoid non-specific amplification and improve the detection sensitivity.

High-flux automatic detection system for food pathogenic bacteria of micro-fluidic chip

Resumen de: CN122042956A

The invention relates to the technical field of food detection, and discloses a high-flux automatic detection system for food pathogenic bacteria of a micro-fluidic chip, which solves the problem that the micro-fluidic chip is inconvenient to quickly clean at present, and comprises a box body and the micro-fluidic chip, the outer side of the box body close to the bottom is fixedly connected with a liquid discharge pipe, and the liquid discharge pipe is fixedly connected with the micro-fluidic chip. A cleaning mechanism is arranged on the box body, a clamping mechanism is arranged in the box body, a mounting mechanism is arranged at the bottom of the micro-fluidic chip, a plurality of reaction cavities are formed in the micro-fluidic chip at equal intervals, a channel is formed in the end part of each reaction cavity, and a plurality of hoses are fixedly connected to the outer side of the micro-fluidic chip at equal intervals; one end, far away from the micro-fluidic chip, of each hose is fixedly connected with a hard tube, and each hose is communicated with each reaction cavity; according to the invention, waste liquid in the reaction cavity and the channel can be rapidly discharged, so that the micro-fluidic chip can be fully and rapidly cleaned without being disassembled.

DETECTION OF AN AMPHIPHILE USING VISUAL INSPECTION OF A LIGAND-MODIFIED SUBSTRATE

Resumen de: US20260133191A1

Disclosed herein are compositions, devices, systems, and methods for detection of an amphiphile using visual inspection of a ligand-modified substrate. For example, disclosed herein are assays for detection of an amphiphile via visual inspection, the assay comprising a ligand-modified substrate and a film of lubricant disposed on the ligand-modified substrate. Also disclosed herein are methods of use of any of the assays disclosed herein. In some examples, the methods comprise contacting any of the assays disclosed herein with a liquid sample; tilting the assay; and visually inspecting the liquid sample disposed on the assay to determine a property of the liquid sample. In some examples, the amphiphile comprises a biological amphiphile, such as an endotoxin. In some examples, the biological amphiphile comprises an endotoxin secreted by a gram-negative bacteria, such as Escherichia coli.

METHODS AND MEANS FOR DETECTION OF LEGIONELLA

Resumen de: EP4488391A1

The invention relates to methods for determining whether a sample comprises Legionella pneumophila, the method comprising performing an isothermal nucleic acid amplification (iNAAT) reaction, preferably loop-mediated isothermal amplification (LAMP), with said sample with a primer set specific for the Legionella pneumophila DotB or MIP gene and determining whether the sample comprises an amplification product of the amplification reaction. The invention further relates to primer sets specific for the Legionella pneumophila DotB or MIP gene that are useful in such method, and kits of part comprising such primer set.

A SYSTEM FOR RAPIDLY DETECTING AND IDENTIFYING MICROORGANISMS ON BIOLOGICAL SURFACES (E.G. FISH SKIN SURFACES) AND/OR NON-BIOLOGICAL SURFACES (E.G. HABITAT OR IC SURFACES) WITH A FLEXIBLE DISSOLVABLE CLOTH AND CORRESPONDING METHODS

Resumen de: EP4741805A1

0001 The current invention comprises systems and methods for the detection of food related bacteria, such as listeria, e. coli and s. aureus in a mobile and modular design. The combination of a dissolvable flexible cloth as sampling means, a multiple target sensor, smart readout optics and spectral processing enables to instantly ascertain, verify and validate the presence of specific microbes. The main application is the monitoring of the food and food production in order to prevent the coming to the market of bacteria invested products, such as fish, chicken or other food products destined for human consumption.

RPA-CRISPR/Cas12a-based magnetic control ratio fluorescent biosensor as well as preparation method and application thereof

Resumen de: CN122016744A

The invention provides a magnetic control ratio fluorescent biosensor based on RPA-CRISPR/Cas12a as well as a preparation method and application of the magnetic control ratio fluorescent biosensor, and belongs to the technical field of fluorescent biosensors and pathogenic bacterium detection. The magnetic control ratio fluorescence biosensor comprises a fluorescence reference signal element, a fluorescence response signal element and a specific recognition element, according to the magnetic control ratio fluorescent biosensor, high-sensitivity and specific detection of staphylococcus aureus and salmonella is realized through the magnetic and fluorescent characteristics of the fluorescent reference signal element, the fluorescent characteristic of the fluorescent response signal element and the nucleic acid cleavage activity of Cas12a; the magnetic control ratio fluorescent biosensor has a built-in calibration function, can reduce interference brought by the environment and instruments, has good selectivity to staphylococcus aureus and salmonella, has good anti-interference performance to other bacteria, and can be used for rapid detection in the field of food safety.

Primer pair, composition, kit and method for detecting pseudomonas plecoglossicida causing large yellow croaker visceral white-spot disease based on RPA-CRISPR/Cas12a technology

Resumen de: CN122012760A

The invention relates to a primer pair, a composition, a kit and a method for detecting pseudomonas plecoglossicida causing large yellow croaker visceral white-spot disease based on an RPA-CRISPR/Cas12a technology, the nucleotide sequences of upstream primers RPA-F are respectively as shown in SEQ ID NO.1-4, the nucleotide sequences of downstream primers RPA-R corresponding to the upstream primers RPA-F are respectively as shown in SEQ ID NO.5-8, and the composition comprises the primer pair, crRNA, Cas12a protein and a fluorescent reporter probe. Compared with the prior art, the invention establishes a P. plecoglossicida detection method which is simple and convenient to operate, short in detection time, good in specificity and high in sensitivity and only needs common constant-temperature equipment, and is suitable for on-site rapid detection in basic laboratories and large yellow croaker farms.

Real-time fluorescent quantitative PCR (polymerase chain reaction) detection method for total RNA (ribonucleic acid) residual quantity of escherichia coli

Resumen de: CN122012677A

The invention provides a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection method for total RNA (Ribonucleic Acid) residual quantity of escherichia coli. A reaction system comprises 3-5 mu L of Taqman RT-PCR Buffer (5 *), 1-3 mu L of TaqMan Enzyme Mix (10 *), 0.3-0.5 mu L of ROX Reference Dye (50 *), 1-1.5 mu L of 8-12 mu M forward specific primer, 1-1.5 mu L of 8-12 mu M reverse specific primer, 0.5-0.8 mu L of 8-12 mu M specific probe, 4-6 mu L of standard substance or to-be-detected sample and RT-PCR-grade water which is supplemented to 20 mu L, and a reaction procedure is as follows: the temperature is 52-54 DEG C for 4-6 minutes; the temperature is 94-96 DEG C for 18-22 s; the detection time is 4-6 seconds at the temperature of 94-96 DEG C, 38-42 seconds at the temperature of 56-58 DEG C and 44-46 cycles, and the detection limit of the method is as low as 2 pg/mL.

Multiple primer combination and method for identifying mycobacteria and respiratory tract pathogenic bacteria and detecting drug-resistant genes of mycobacteria and respiratory tract pathogenic bacteria

Resumen de: CN122012685A

The invention relates to the technical field of detection, and provides a multiple primer combination and method for identifying mycobacteria and respiratory tract pathogenic bacteria and detecting drug-resistant genes of the mycobacteria and the respiratory tract pathogenic bacteria, and the nucleotide sequence of the primer combination comprises sequences as shown in SEQ ID NO: 1-SEQ ID NO: 380; the method comprises the following steps: nucleic acid extraction: extracting DNA and RNA in a sample as templates; reverse transcription: reversely transcribing the RNA into DNA (Deoxyribonucleic Acid); carrying out multi-target amplification, and configuring multi-PCR amplification; linker connection and library purification; and performing machine sequencing and data analysis. According to the application, pathogen identification is more comprehensive and accurate, and five kinds of mycobacterium tuberculosis, 34 kinds of nontuberculous mycobacteria and 55 kinds of common respiratory tract pathogenic bacteria are included; 2000 drug-resistant gene loci of clinically used anti-tuberculosis first-line and second-line drugs are comprehensively covered; all drug-resistant gene loci recommended by WHO and a guide can be comprehensively detected; the sensitivity and the specificity are high.

Preparation method and application of flat plate for detecting minimum inhibitory concentration of fosfomycin by simple agar dilution method

Resumen de: CN122012668A

The invention discloses a preparation method and application of a flat plate for detecting the minimum inhibitory concentration of fosfomycin by a simple agar dilution method. The method comprises the following steps: dissolving fosfomycin and glucose-6-phosphoric acid dry powder in a small amount of sterile water in advance according to a target concentration, quantitatively coating the surface of solidified agar with the dissolved fosfomycin and glucose-6-phosphoric acid dry powder, and uniformly infiltrating the fosfomycin into the agar after drying so as to form a flat plate with a specific concentration gradient. The minimum inhibitory concentration of fosfomycin is detected through the flat plate provided by the invention, and compared with a standard agar dilution method, the basic consistency of the method reaches 100%, the classification consistency reaches 99%, and medicine distribution is uniform through verification of 100 strains of clinical urinary tract infection Escherichia coli. The method abandons tedious steps of preparing agar from zero in a traditional method, is simple and convenient to operate, is accurate and reliable, solves the technical problems that a traditional agar dilution method is tedious in operation and short in plate storage life, and provides an efficient and standardized fosfomycin drug sensitivity detection scheme for a clinical microbiological laboratory.

Method for rapidly and ultrasensitively detecting escherichia coli O157: H7 based on SERS (Surface Enhanced Raman Scattering)

Resumen de: CN121995052A

The invention belongs to the technical field of biological detection, and particularly discloses a method for rapidly and ultrasensitively detecting escherichia coli O157: H7 based on SERS (Surface Enhanced Raman Scattering). Comprising the following steps: preparing immunomagnetic bead capture nanoparticles, preparing precious metal or precious metal composite nanoparticle sol, preparing immune precious metal or precious metal composite signal nanoparticles, preparing a sandwich structure, and detecting escherichia coli O157: H7. According to the method, the antibody serves as a specific recognition element and is specifically combined with and used for measuring escherichia coli O157: H7, on one hand, the dispersed state of a nano material in a detection system can be stabilized, on the other hand, target protein can be recognized with high specificity and high affinity, and the method is good in stability, low in preparation cost and suitable for large-scale popularization and application. The detection accuracy is improved to a great extent; the method is higher in detection speed, extremely simple in detection process, short in time consumption, suitable for field rapid detection and batch screening, low in detection limit, ultra-high in detection speed and ultra-high in detection sensitivity, and capable of accurately recognizing trace target thalli.

Nucleic acid warm-expansion detection system, detection method and application of nucleic acid warm-expansion detection system

Resumen de: CN121992078A

The invention discloses a nucleic acid warm-amplification detection system, a detection method and application thereof, and develops a multi-form, rapid and sensitive RH-RPA detection method by combining escherichia coli RNase HII with an RPA/RT-RPA technology. According to the RH-RPA method, a long probe containing single ribonucleotide is used as a substrate for specific cutting of RNase HII, so that fluorescence visualization is realized. Besides, the long probe can be used as a forward primer after being cut, the forward primer and a reverse primer with a biomarker extend to generate a product with double markers, and rapid detection can be performed through lateral flow chromatography. Compared with the conventional common RPA-exo and RPA-nfo methods, the method disclosed by the invention has the advantages that the detection of a real-time fluorescence method and a lateral flow chromatography method can be realized at the same time under a low-temperature condition, the method is rapid, stable and accurate, the defects of the RPA probe method are overcome, and the practical application performance of RPA detection is greatly improved.

Method for rapidly and sensitively detecting pathogenic bacteria based on identification-amplification space-time isolation strategy of M13 bacteriophage biological lever

Resumen de: CN121992077A

Bacterial infection detection is often limited by the sensitivity of a traditional immunoassay, and is derived from signal load-recognition efficiency tradeoff caused by carrier diffusion limitation and steric hindrance. Therefore, an M13 bacteriophage mediated'biological lever 'system is constructed, recognition and signal amplification are separated in space, pIII protein displays a nano antibody to realize target specific capture, pVIII protein is covalently connected with a high-density DNA probe to serve as a signal interface, low-leakage catalytic hairpin assembly (CHA)-CRISPR/Cas13a cascade amplification (LUCAS) is triggered, and a single recognition event is converted into multi-stage signal enhancement. The design effectively improves transduction efficiency and inhibits background interference. Acinetobacter baumannii is taken as a model, and the system realizes the detection limit of 10 CFU/mL within 2 hours, is stable in labeled bronchoalveolar lavage fluid and has no cross reaction to common pathogens. The strategy provides powerful support for sensitive and rapid detection of pathogens.

REACTION MEDIUM AND METHOD FOR DETECTING SHIGA TOXIN-PRODUCING E.COLI AND/OR ENTEROHAEMORRHAGIC E.COLI

Resumen de: WO2025003117A1

The present invention relates to a gelled reaction medium for detecting, identifying, and/or isolating at least one Shiga toxin-producing strain of E. coli, the reaction medium comprising: - at least one toxin inducer, - at least one agglutinating conjugate comprising at least one specific binding partner of STX1 and/or at least one specific binding partner of STX2, coupled to a nanoparticle; - a concentration gradient of a compound for inhibiting non-target bacteria. The present invention also relates to the associated method for detecting and/or isolating Shiga toxin-producing E. coli which is likely to be present in a sample comprising enterobacteria.

METHOD FOR DETECTING AND CONFIRMING SHIGA TOXIN-PRODUCING ESCHERICHIA COLI AND/OR ENTEROHAEMORRHAGIC E. COLI

Resumen de: WO2025003119A1

The invention relates to a method for detecting and confirming at least one Shiga toxin-producing Escherichia Coli (STEC) which may be present in a sample comprising enterobacteria, comprising the following steps: - performing lysis of the sample, enabling lysis of the STECs in order to obtain a solution comprising the nucleic acids thereof; - bringing the solution of nucleic acids into contact with primers, making it possible to amplify at least the stx1 and/or stx2 gene or gene fragment; - if at least one of the stx1 and/or stx2 genes or gene fragments is amplified, part of the sample is deposited on an agar reaction medium comprising ■ at least one toxin inducer, ■ at least one agglutinating conjugate formed by at least one binding partner specific to the STX1 protein and/or at least one binding partner specific to the STX2 protein, which binding partner(s) is (are) coupled to a nanoparticle; - detecting and confirming the presence of at least one STEC by the appearance of a halo on the agar around the STEC.

Primer group for simultaneously detecting four types of horse digestive tract bacteria and application of primer group

Resumen de: CN121975962A

The invention discloses a primer group for simultaneously detecting four equine digestive tract bacteria and application thereof, the primer group comprises primer sequences as shown in SEQ ID NO: 1-8, and the four equine digestive tract bacteria are salmonella enteritidis, salmonella typhimurium, clostridium difficile and lawsonia intracellular. Extracting total DNA (deoxyribonucleic acid) of a sample to be detected by using the excrement sample nucleic acid extraction kit; the total DNA is used as a template, and the primer group is used for multiple PCR reaction to obtain an amplification curve. According to the invention, a primer sequence with high sensitivity and specificity is adopted, so that the quality of a detection result is ensured; the detection method is simple to operate, time-saving and labor-saving; the detection flux is high, and the reagent consumable cost is low.

Pseudomonas aeruginosa drug resistance gene detection primer group based on multiple PCR-time-of-flight mass spectrometry, kit and kit use method

Resumen de: CN121975958A

The invention discloses a pseudomonas aeruginosa drug resistance gene detection primer group based on multiple PCR-time-of-flight mass spectrometry, a kit and a use method of the kit. The pseudomonas aeruginosa drug-resistant gene comprises armA, rmtB, rmtC, mexA, blaVIM, blaNDM, blaIMP, blaKPC, blaOXA and blaGES, and the pseudomonas aeruginosa drug-resistant gene is a drug-resistant gene of pseudomonas aeruginosa. The detection primer group comprises upstream and downstream amplification primers of each gene and a single-base extension primer; the nucleotide sequences of upstream and downstream amplification primers of each gene are respectively as shown in SEQ ID NO.1-NO.20; nucleotide sequences of single base extension primers of all the genes are shown as SEQ ID NO. 21 to NO. 30 respectively. By adopting the kit comprising the primer group, whether pseudomonas aeruginosa infection exists or not can be known within 40 minutes, and if the pseudomonas aeruginosa infection exists, the drug resistance condition of the pseudomonas aeruginosa can be known. The detection method has high sensitivity and specificity in the detection process, the sample detection accuracy rate can reach 92-97%, and the method can be used for rapid, accurate, high-sensitivity and high-throughput detection of the pseudomonas aeruginosa antibiotic-resistant strains and is beneficial to application and popularization in laboratories of hospitals and grass-roots departments.

Microflora prediction model for detecting premature delivery risk and kit and application thereof

Nº publicación: CN121975957A 05/05/2026

Solicitante:

SHANGHAI FIRST MATERNITY AND INFANT HOSPITAL
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Resumen de: CN121975957A

The invention relates to a flora prediction model for detecting premature delivery risk and a kit and application thereof. Through early-stage mNGS data collection and analysis, premature related strains are obtained, and through a large number of experimental screening and optimization, a set of optimal primer probe group is finally determined. The invention relates to a premature delivery risk calculation method, which comprises the following steps of: selecting 12 floras, namely lactobacillus crispatus, lactobacillus gasseri, lactobacillus inertus, lactobacillus jensenii, escherichia coli, enterococcus faecalis, enterobacter aerogenes, group B hemolytic streptococcus, ureaplasma parvum, chlamydia trachomatis, diplococcus gonorrhoeae and gardnerella vaginalis, and establishing a fluorescent quantitative PCR (Polymerase Chain Reaction) method and a premature delivery risk calculation model aiming at the detection of the 12 floras. Compared with an existing detection method, the method is simple, convenient, rapid, high in sensitivity and high in specificity, the false positive and false negative risk is reduced, and the purpose of batch detection is achieved. Clinically, the change of microbial flora in the reproductive system of a pregnant woman can be rapidly detected, corresponding treatment measures are taken, the risk of PTB occurrence is reduced, and the kit has a good application prospect.