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METHODS AND SYSTEMS FOR THE RAPID DETECTION OF SALMONELLA USING INFECTIOUS AGENTS

Resumen de: MX2025013800A

Disclosed herein are methods and systems for rapid detection of microorganisms in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Salmonella-specific bacteriophage, allows detection of a specific microorganism, such as Salmonella spp. and an indicator signal may be amplified to optimize assay sensitivity.

Primer and probe combination for simultaneously detecting urinary tract klebsiella pneumoniae, escherichia coli and drug-resistant genes through nested PCR (polymerase chain reaction) and kit of primer and probe combination

Resumen de: CN121951097A

The invention relates to a primer and probe combination for simultaneously detecting urinary tract klebsiella pneumoniae, escherichia coli and drug-resistant genes through nested PCR (Polymerase Chain Reaction) and a kit of the primer and probe combination, which are characterized in that a phoE target gene is selected for primer and probe design to ensure that cross amplification of related bacteria is not caused, so that the detection specificity requirement of actual klebsiella pneumoniae is met; the Klebsiella pneumoniae and the escherichia coli can be identified at the same time, the source of the drug-resistant gene KPC can be judged, and the method is very important for clinical precise diagnosis and treatment.

Bacteria visual detection and in-situ sterilization kit and method and application thereof

Resumen de: CN121950996A

The invention discloses a bacterium visual detection and in-situ quantitative sterilization method and application, and relates to the field of bacterium detection and sterilization. According to the method, liquid crystal double-emulsion (LCDE) is taken as a carrier, hCG, alpha-amylase and kanamycin are loaded, and bacterium visual detection and in-situ quantitative sterilization are realized by combining a host-guest competition effect and a pregnancy test strip (PTS). The pyrene-modified single strand and the LPS aptamer are hybridized to form double strands, when a target (LPS or bacteria) exists, the aptamer is specifically bound with the double strands to release the pyrene-modified single strand, the pyrene-modified single strand competes to occupy a beta-CD cavity and release CTAB, and the stability of an LCDE interface is destroyed to release an internal drug carrier; exo I shears single-stranded DNA, alpha-amylase hydrolyzes beta-CD, and the Exo I and the alpha-amylase synergistically amplify signals. The technology is excellent in detection performance, the LPS detection limit reaches 0.8 ng/mL, and the escherichia coli detection limit is 1.2 CFU/mL; the kanamycin is released according to needs, the sterilization rate exceeds 80%, the problems of environmental pollution and drug resistance caused by excessive drugs can be avoided, operation is easy and convenient, stability is good, and the method has great application value in multiple fields.

Preparation method of SERS (Surface Enhanced Raman Scattering) sensor based on oversized annular DNAzyme, product and application of SERS sensor in detection of escherichia coli O157: H7

Resumen de: CN121955375A

The invention discloses a preparation method of an SERS (Surface Enhanced Raman Scattering) sensor based on oversized annular DNAzyme, a product and application of the SERS sensor in escherichia coli O157: H7 detection, and relates to the technical field of nano materials and biological detection. The SERS sensor disclosed by the invention comprises an aptamer, namely a Key chain-Link chain three-chain body, an LDNAzyme-Block chain double-chain body, an rA Hairpin, an Au-H1 Hairpin probe, an H2 Hairpin probe and an AuNPs-H3 Hairpin probe, and the SERS sensor disclosed by the invention is characterized in that the SERS sensor comprises an aptamer, namely a Key chain-Link chain three-chain body, an LDNAzyme-Block chain double-chain body, an rA Hairpin probe and an AuNPs-H3 Hairpin probe; in the presence of escherichia coli O157: H7, a bimorphic gold nano assembly probe can be formed; the preparation method of the SERS sensor is simple and easy to control, the bimorphic gold nano assembly probe formed after target recognition has a Y-shaped structure with 3D space constraint, the stability is high, the detection operation on escherichia coli O157: H7 is simple and convenient, the sensitivity is high, the detection limit is low, and the specificity is high.

Salmonella detection instrument and method for livestock and poultry breeding based on biomedicine

Resumen de: CN121950481A

The invention relates to the technical field of biomedicine detection, and discloses a biomedicine-based salmonella detection instrument and method for livestock and poultry breeding, the biomedicine-based salmonella detection instrument comprises a detection table and an electron microscope mounted at the upper end of the middle part of the detection table, a conveying assembly is arranged at the upper end of the detection table, and the conveying assembly is used for continuously feeding a culture dish; and the conveying assembly comprises two groups of rotating rods connected with the detection table. According to the salmonella detection instrument and method for livestock and poultry breeding based on biomedicine, stable power is provided for the conveying assembly through the first motor, and continuous and automatic conveying of culture dishes is achieved in cooperation with transmission of a synchronous belt wheel and a feeding belt; the feeding assembly synchronously completes accurate feeding of single culture dishes through the power of the conveying assembly by means of linkage of a driving belt wheel, a driven belt wheel and a transmission belt, a power source does not need to be additionally arranged, the structure is simplified, and meanwhile cooperation and consistency of the feeding rhythm and the conveying rhythm are achieved; and the detection requirements of large-batch salmonella in large-scale livestock and poultry breeding are effectively met.

Method for detecting food-borne pathogenic bacteria based on Argonaute protein in combination with loop-mediated isothermal amplification and electrochemical biosensing

Resumen de: CN121951013A

The invention discloses a method for detecting food-borne pathogenic bacteria based on Argonaute protein in combination with loop-mediated isothermal amplification and electrochemical biosensing, and belongs to the technical field of microbiological detection and biosensing. The integrated detection method based on Argonaute protein, loop-mediated isothermal amplification and electrochemical biosensing, which is high in sensitivity, strong in specificity, simple and convenient to operate and suitable for on-site rapid detection, is provided aiming at the problems that an existing food-borne pathogenic bacterium detection method is insufficient in sensitivity, depends on complex instruments, is difficult to apply on site and the like. Target nucleic acid is subjected to exponential amplification through LAMP (loop-mediated isothermal amplification), then Argonaute-mediated two-stage specific recognition and signal transduction are performed, and finally reading is performed through a high-sensitivity electrochemical platform, so that the detection limit of salmonella typhimurium as high as 1 CFU/mL is realized, and the detection range can cover 100-108 CFU/mL.

Preparation method of ratio type electrochemical luminescence biosensor, sensor and application of sensor in detection of escherichia coli O157: H7

Resumen de: CN121955376A

The invention discloses a preparation method of a ratio type electrochemical luminescence biosensor, the sensor and application of the sensor in detection of escherichia coli O157: H7, and relates to the technical field of nano materials and biological detection. The sensor provided by the invention comprises an Aptamer-Templet double-strand body, a phi29 polymerase, a Nb.BbvCI nicking enzyme, a probe 1 and a probe 2, wherein the probe 1 and the probe 2 are connected with each other; the method comprises the following steps: specifically recognizing surface protein of escherichia coli O157: H7, releasing a Templet chain, releasing a Trigger chain and opening a hairpin structure of a probe 1 under the assistance of polymerase and cutting enzyme, and opening a hairpin structure of a probe 2 by the opened probe 1, so that self-assembly of the probe 1 and the probe 2 is realized, and a detectable object is obtained; the detection limit of the sensor to escherichia coli O157: H7 is as low as 1 CFU/mL, and the sensor is wide in linear range, high in stability, suitable for multiple samples, high in specificity and easy and convenient to use and operate.

Rapid detection kit for bacterial food-borne pathogenic microorganisms and application of rapid detection kit

Resumen de: CN121951093A

The invention relates to the technical field of biology, in particular to a rapid detection kit for bacterial food-borne pathogenic microorganisms and application of the rapid detection kit. The invention develops a primer probe combination kit for detecting food-borne bacteria salmonella, staphylococcus aureus, vibrio parahaemolyticus, listeria monocytogenes, escherichia coli O157 and clostridium perfringens through optimization of primers, optimization of hydrogel components and a system and the like. The primer probe combination kit is used for detecting food-borne bacteria salmonella, staphylococcus aureus, vibrio parahaemolyticus, listeria monocytogenes, escherichia coli O157 and clostridium perfringens. The kit is used for detecting salmonella, staphylococcus aureus, vibrio parahaemolyticus, listeria monocytogenes, Escherichia coli O157 and clostridium perfringens. The kit is high in sensitivity and specificity, strong in anti-interference capability, excellent in stability and portability, rapid and efficient in detection, simple and convenient to operate and low in cost, and can realize result visualization and relative quantification, so that the kit has wide application prospects and values.

Primer probe combination, kit, nucleic acid detection method and application

Resumen de: CN121931266A

The invention provides a primer probe combination, a kit, a nucleic acid detection method and application, and relates to the technical field of biochemical detection. The primer probe combination comprises a first primer pair and a first probe, wherein the first primer pair and the first probe are used for specifically amplifying pseudomonas aeruginosa; the second primer pair and the second probe are used for specifically amplifying enterococcus; the third primer pair and the third probe are used for specifically amplifying staphylococcus aureus; the fourth primer pair and the fourth probe are used for specifically amplifying the salmonella enterica. According to the primer and probe combination, a specific nucleotide sequence is utilized, efficient and accurate recognition of pseudomonas aeruginosa, enterococcus, staphylococcus aureus and salmonella enterococcus is achieved in a complex intestinal environment, and non-specific interference is effectively avoided; meanwhile, synchronous quantitative detection of four pathogens is supported, the detection efficiency is remarkably improved, and an accurate and sensitive detection means is provided for rapid clinical evaluation of flora conditions.

Micro-fluidic chip for simultaneously detecting multiple food-borne pathogenic bacteria as well as preparation method and application of micro-fluidic chip

Resumen de: CN121927707A

The invention relates to a micro-fluidic chip capable of simultaneously detecting multiple food-borne pathogenic bacteria as well as a preparation method and application of the micro-fluidic chip, in particular to detection of the food-borne pathogenic bacteria, and belongs to the technical field of analysis and detection. The device comprises a functionalized glass slide, a PDMS chip layer and a copper net, the functionalized glass slide is located at the bottommost layer, and a PDMS chip layer in plasma bonding connection is arranged on the functionalized glass slide; a plurality of through holes are formed in the PDMS chip layer, and a copper net is placed in each through hole; a plurality of round holes with the same diameter are formed in the copper net. The micro-fluidic chip is prepared through preparation of a functionalized glass slide, preparation of a PDMS chip layer, preparation of a detection unit and training of a deep learning model and is used for detecting various food-borne pathogenic bacteria at the same time. The liquid crystal biosensor provided by the invention has the advantages of simplicity, portability, rapidness, sensitivity, low cost, no mark, visualization and the like. The method has a good practical application prospect in the fields of rapid detection and the like.

Electrochemical sensor for detecting escherichia coli in food and preparation method of electrochemical sensor

Resumen de: CN121917619A

The invention belongs to the technical field of harmful microorganism detection, and particularly provides an electrochemical sensor for detecting escherichia coli (escherichia coli O157: H7) in food and a preparation method of the electrochemical sensor. According to the electrochemical sensor, the surface of a substrate electrode is sequentially modified with an electron transduction layer and a recognition molecular layer; wherein the electron transduction layer is formed by an infinite coordination polymer composite material of gold nanoparticles; the recognition molecular layer is a nucleic acid aptamer. The electrochemical sensor is high in sensitivity, good in selectivity and low in detection limit; compared with other detection methods, the method does not need incubation and can be directly used for in-situ and rapid detection of bacteria.

Application of electrochemical active bacteria sensitive to organochlorine pollutants in detection of biotoxicity of water quality

Resumen de: CN121917621A

The invention discloses application of electrochemical active bacteria sensitive to organochlorine pollutants in detection of water quality biotoxicity, and belongs to the technical field of water quality biotoxicity detection.After a bacteria solution of the electrochemical active bacteria is exposed in toxic pollutants, the water quality biotoxicity is determined according to an electric signal inhibition rate; the toxic pollutants are organic chlorine pollutants; the electrochemical active bacterium is a pseudomonas genus, and specifically, the pseudomonas genus is Pseudomonas lopnurensis or Pseudomonas delhiensis. The invention further discloses a preparation method of the electrochemical active bacterium. The method provided by the invention is expected to improve the sensitivity of determining the biotoxicity of the organic chlorine pollutants, overcome the application bottleneck of low sensitivity, and provide technical support for biotoxicity monitoring work.

Primer and method for detecting salmonella and drug-resistant gene thereof and application of primer and method

Resumen de: CN121915177A

The invention belongs to the technical field of microbiological detection, and particularly relates to a primer and a method for detecting salmonella and a drug-resistant gene thereof and application of the primer and the method. The gene of the salmonella is invA, and the drug resistance genes are blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA; the gene of the salmonella is invA, and the drug resistance genes of the salmonella are blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA; the primers comprise inner primers and outer primers of invA, blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA, a primer group is designed according to conserved regions of a salmonella invA gene and part of beta-lactam drug-resistant genes blaNDM-1, blaTEM-1, blaOXA and blaCTX-M, a detection method of SYBR-LAMP and HNB-LAMP technologies is established, and screening of part of beta-lactam drug-resistant genes carried by animal-derived pathogenic strains is realized.

Aquatic product quality safety detection device for rapidly screening food-borne pathogenic bacteria

Resumen de: CN121899348A

The invention relates to the technical field of aquatic product quality safety detection, in particular to an aquatic product quality safety detection device for rapidly screening food-borne pathogenic bacteria. Comprising a workbench, a support, a suction head bin, a sample frame, a detection module, a suction head recovery box, a movement module and a pipetting module, the support is fixedly arranged at the top of the workbench, and the suction head bin, the sample frame, the detection module and the suction head recovery box are arranged between the workbench and the support and are sequentially arranged from left to right; the movement module is fixedly arranged at the inner top of the bracket, and the pipetting module is movably arranged below the movement module; an integrated, processized and automatic detection platform is constructed, the complete process from suction head installation, sample/reagent suction, liquid transfer and distribution to final suction head discarding is automatically completed, instability and fatigability of manual operation are avoided, and the overall detection efficiency and consistency are remarkably improved.

Three-dimensional fluorescence detection method and system for identifying escherichia coli in bile

Resumen de: CN121899099A

The invention relates to the technical field of visible light absorption spectrum detection, in particular to a three-dimensional fluorescence detection method and system for identifying escherichia coli in bile, and the method comprises the following steps: generating a light source through a light source generation module; adjusting the intensity of the light source by using a light splitting module, and dividing the light source into two paths of light so as to obtain sample detection light and a reference spectrum; acquiring a background spectrum and a bile detection spectrum by utilizing a sample detection module based on the sample detection light; according to the reference spectrum, the background spectrum and the bile detection spectrum, the upper computer uses a spectrum detection model to identify escherichia coli in the bile sample. Based on the detection principle of three-dimensional fluorescence, the intelligent degree and efficiency of detection of escherichia coli in bile can be improved, and the detection cost can be reduced.

Semi-quantitative detection kit for clostridium perfringens, detection method and application

Resumen de: CN121896313A

The invention belongs to the technical field of microbiological detection, and particularly provides a clostridium perfringens semi-quantitative detection kit, a detection method and application, the clostridium perfringens semi-quantitative detection kit comprises a liquid reagent A and a solid reagent B; the liquid reagent A comprises an aqueous solution in which tryptone, soy peptone, yeast extract powder and ammonium ferric citrate are dissolved; the solid reagent B comprises sodium pyrosulfite. According to the clostridium perfringens semi-quantitative detection kit provided by the invention, the specific liquid culture medium with an optimized formula is adopted, and the reagent A and the reagent B adopt a liquid-solid split charging design, so that sodium pyrosulfite is prevented from being degraded in a liquid environment, and the shelf life is prolonged. And a standard colorimetric card and a time comparison table can be prepared, so that subjective errors are reduced. In addition, the device can be integrated with a disposable sampling tool, a portable constant-temperature water bath cup and a timing prompt assembly to form a complete detection system which can be used after being opened, and the single detection cost is low.

SERS method for high-sensitivity detection of aquatic product pathogenic bacteria

Nº publicación: CN121899103A 21/04/2026

Solicitante:

DONGSHAN CUSTOMS COMPREHENSIVE TECHNICAL SERVICE CENTER
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Resumen de: CN121899103A

According to the scheme, target bacteria (such as vibrio parahaemolyticus) are subjected to high-sensitivity SERS detection by utilizing a sandwich structure principle, Au (at) Ag-MPBA/PEG-Ab1 is prepared as an SERS probe for quantitative detection of the target bacteria, and enrichment of the target bacteria is effectively realized by combining a magnetic composite nano material Fe3O4 (at) TA/Ag NPs-Ab2, so that the SERS detection of the pathogenic bacteria in the aquatic products is realized. A sandwich structure detection system with high sensitivity and specificity to vibrio parahaemolyticus is formed, finally, signal amplification is achieved by preparing a two-dimensional flexible functional regenerated SERS substrate of a high-density SERS'hot spot 'with a bamboo cellulose membrane as a raw material, so that enrichment and sensitivity detection of vibrio parahaemolyticus is rapidly completed, the detection range is 10-107 CFU/mL, and the detection sensitivity is high. The method has great potential in the aspects of food safety monitoring and infectious disease diagnosis.