Alerta

Florfenicol nano-liposome modified by lactoferrin as well as preparation method and application of florfenicol nano-liposome

Resumen de: CN120960461A

The invention discloses a lactoferrin modified florfenicol nano-liposome (R-FL) as well as a preparation method and application thereof. The R-FL is prepared by adopting a technology of combining an improved reversed-phase solvent evaporation method and chemical coupling. The R-FL disclosed by the invention is strong in slow and controlled release capability and good in biocompatibility, has excellent in-vitro and in-vivo antibacterial effects, and can be used for inhibiting the formation of a biofilm by precisely damaging the structure of the biofilm of the drug-resistant salmonella typhimurium in a targeting manner. The R-FL can effectively regulate the intestinal flora disorder caused by the drug-resistant salmonella typhimurium, so that the intestinal flora is recovered to a normal level. The R-FL can be used as a novel nano preparation with great potential for resisting drug-resistant salmonella typhimurium disease, and has a wide application prospect.

Lactobacillus plantarum strain LM2 and application thereof

Resumen de: CN120966672A

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LM2 and application of the lactobacillus plantarum strain LM2. The strain has relatively high GABA (gamma-aminobutyric acid) production capacity and nitrite degradation capacity, has a bacteriostatic effect on intestinal pathogens such as staphylococcus aureus, salmonella enteritidis, listeria monocytogenes and campylobacter jejuni, has a good bacteriostatic function, also has good acid resistance and cholate resistance, and can be used for preparing a bacterial strain for treating intestinal diseases. The strain can well survive in artificial intestinal juice and artificial gastric juice; in order to increase the yield of GABA, the accelerant capable of remarkably promoting the growth of the strain LM2 is obtained by researching the accelerant, and the highest survival rate of the freeze-dried powder prepared by optimizing the accelerant and the freeze-dried powder can reach 99% or above, the nitrite degradation rate can reach 99% or above, the yield of GABA is increased to 3.0 g/L or above, and the freeze-dried powder is bacterial powder capable of being widely applied.

Pediococcus pentosaceus TRM54998 with broad-spectrum antibacterial activity and application thereof

Resumen de: CN120966712A

The invention discloses pediococcus pentosaceus TRM54998 with broad-spectrum antibacterial activity and application of the pediococcus pentosaceus TRM54998, and belongs to the field of microorganisms. The strain is preserved in the China Center for Type Culture Collection (CCTCC) on June 04, 2024, and the preservation number is CCTCC NO: M 20241142. It is proved that the strain has good acid resistance and bile salt resistance and can grow in the environment with the bovine bile salt concentration being 0.1-0.2% (w/v) or in the pH value range being 3-7; the fermentation supernatant has an inhibition effect on nine pathogenic bacteria such as salmonella and the like, and the antibacterial effect is stable; meanwhile, experiments prove that the pediococcus pentosaceus TRM54998 has no wide drug resistance, can relieve the problems of aggravated drug resistance, damage to microbial ecological balance and the like caused by antibiotic abuse, provides a strain resource for preparing a biological bacteriostatic agent or an intestinal probiotic agent, and has a wide application prospect.

Bacteriostatic composition, and bacterial agent, veterinary drug and feed containing same

Resumen de: CN120960268A

The invention relates to the technical field of nano engineering and probiotics, in particular to a bacteriostatic composition and a fungicide, a veterinary drug and a feed containing the bacteriostatic composition. The invention provides a bacteriostatic composition prepared by simultaneously mixing nano iron sulfide and lactobacillus, which can protect the intestinal colonization ability of lactobacillus and other beneficial bacteria, so that the nano iron sulfide promotes bacterial ferroptosis of pathogenic bacteria in the intestinal tract. The nano iron sulfide and the lactobacillus synergistically inhibit the growth of pathogenic bacteria such as escherichia coli, salmonella typhimurium and staphylococcus aureus in vitro and in intestinal tracts, have no adverse effect on beneficial bacteria and host intestinal epithelial cells, and are beneficial to intestinal health. The invention provides a natural and efficient application mode, and has very high conversion value.

Method and kit for simultaneously detecting avian leukosis virus, mycoplasma synoviae and salmonella pullorum antibodies

Resumen de: CN120971727A

The invention discloses a method and a kit for simultaneously detecting avian leukosis virus, mycoplasma synoviae and salmonella pullorum antibodies, and belongs to the technical field of animal epidemic disease detection. According to the method, an avian leukosis virus p27 protein, a chicken mycoplasma synoviae p50 protein and a truncated GroEL-delta8-1 protein of a dominant antigen GroEL of salmonella pullorum are taken as coating antigens, synchronous detection of the three antibodies is realized by optimizing ELSA reaction conditions, and a kit capable of simultaneously detecting the three antibodies is developed at the same time. The kit has the characteristics of strong specificity, high sensitivity and simplicity and convenience in operation, is suitable for rapid screening and purification of poultry epidemic diseases, and reduces the detection cost.

Phenyl thiazolyl benzimidazole compound as well as preparation method and application thereof

Resumen de: CN120965679A

The invention discloses a phenyldihydrothiazolyl benzimidazole compound as well as a preparation method and application thereof, and relates to the technical field of antibacterial drug research. The phenyl dihydrothiazolyl benzimidazole compound prepared by the invention shows broad-spectrum antibacterial activity, has an obvious inhibition effect on staphylococcus aureus, bacillus subtilis, escherichia coli, salmonella and pseudomonas aeruginosa, has the antibacterial effect enhanced along with the increase of the concentration of the compound, shows obvious concentration dependence, and can be used for preparing a compound for treating the pathogenic bacteria. The potential in the development of antibacterial drugs is shown. The compound disclosed by the invention is simple in synthesis process, low in cost, good in repeatability and high in yield, chemical components are easy to control, a new choice is provided for the field of antibacterial drugs, and the compound has important significance for solving the problem of drug resistance of bacteria.

Pharmaceutical composition based on iron-based complex and natural naphthoquinone and application thereof

Resumen de: CN120960256A

The invention discloses a pharmaceutical composition based on an iron-based complex and natural naphthoquinone. The pharmaceutical composition is formed by mixing a coordination polymer and a quinone compound, wherein the coordination polymer is obtained by coordinating epigallocatechin gallate and an iron element. According to the method, a coordination compound formed by natural polyphenol EGCG and Fe is combined with a quinone compound, a novel Fenton reaction system can be constructed, the system uses the quinone compound as a sustainable H2O2 supply source, H2O2 is continuously supplemented in a bacterial microenvironment through quinone redox cycle in an infected microenvironment, and the reaction time is shortened; according to the present invention, the bottleneck of insufficient H2O2 in the infected microenvironment is effectively overcome, the stable substrate is provided for the Fenton reaction, the Fenton reaction circulation is promoted by using the Fe < 3 + > reducing ability of the EGCG so as to produce Fe < 2 + >, and the synergistic interaction effect is generated between the EGCG and the Fe < 2 + > so as to achieve the continuous and efficient generation of OH, such that the removal ability on the salmonella and other drug-resistant bacteria is significantly enhanced;

Weissella fusiformis JWC120 and application thereof

Resumen de: CN120966694A

The invention relates to the technical field of microorganisms, in particular to Weissella fusiformis JWC120 with a function of inhibiting common harmful bacteria in animal breeding and application of the Weissella fusiformis JWC120. The Weissella fusiformis JWC120 with the function of inhibiting common harmful bacteria in animal breeding, provided by the invention, has the preservation number of CGMCC No.31945, and can effectively inhibit common escherichia coli, salmonella, staphylococcus aureus and shigella in animal breeding; a bacteriocin crude extract of the strain has a remarkable inhibition effect on common staphylococcus aureus in animal breeding, and has a good application prospect in animal disease prevention and treatment and development of livestock and poultry breeding health-care products.

Method for detecting food-borne pathogenic bacteria by time-of-flight mass spectrometry

Resumen de: CN120967022A

The invention provides a method for detecting eight food-borne pathogenic bacteria by a time-of-flight mass spectrometry. A primer group comprises a specific primer pair combination of multiple PCR (Polymerase Chain Reaction) of the eight food-borne pathogenic bacteria, wherein the food-borne pathogenic bacteria comprise intestinal aggregation Escherichia coli, intestinal hemorrhagic Escherichia coli, intestinal invasive Escherichia coli, intestinal pathogenic Escherichia coli, Shiga toxin producing Escherichia coli, enterotoxin producing Escherichia coli, listeria monocytogenes and salmonella typhimurium. According to the method, a specific primer is used for performing multiple PCR amplification on pathogen DNA, and the size of a fragment of a multiple PCR product is detected through MALDI-TOF MS. According to the method, multiple PCR and MALDI-TOF MS are combined to be used for identifying clinical pathogenic microorganisms, and the method is rapid, simple, convenient, easy to observe, visual and high in accuracy.

Vanilic acid derived aromatic hydrocarbon ruthenium complex as well as preparation method and application thereof

Resumen de: CN120965770A

The invention discloses a vanillic acid derived aromatic ruthenium complex as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical chemistry and metal complex synthesis. Aiming at the problems of insufficient antibacterial activity and prominent drug resistance of existing antibiotics to multi-drug-resistant and pan-drug-resistant salmonella typhimurium, the method comprises the following steps: synthesizing vanillic acid esterification and etherification derivatives, respectively coupling with 3-picolinic acid and 4-picolinic acid to generate an esterification ligand and an etherification ligand, and then reacting with a dimeric ruthenium precursor in an inert atmosphere to obtain the antibacterial antibiotic for the multi-drug-resistant and pan-drug-resistant salmonella typhimurium. And preparing the aromatic ruthenium complex. According to the method, a ligand structure is modified and optimized through a carboxylic ester bond and an ether bond, and the antibacterial activity is remarkably enhanced by combining the synergistic effect of a ruthenium metal center. The obtained complex has an efficient inhibition effect on clinically separated pan-drug-resistant and multi-drug-resistant salmonella typhimurium, the minimum inhibitory concentration (MIC90) of the complex is as low as 0.06 mu g/mL, and the complex is low in hemolytic toxicity and suitable for developing novel drugs for resisting drug-resistant bacterial infec

粘液を透過するのを制限するための合成結合剤

Resumen de: CN117838855A

The invention relates to a synthetic adhesive for limiting passage through mucus. Synthetic adhesives for reducing the proportion of targets that can traverse mucus and/or free cleavage are disclosed, as well as methods of using these synthetic adhesives to reduce mucosal penetration and/or free cleavage of targets.

Veterinary drug compound preparation as well as preparation method and application thereof

Resumen de: CN120960359A

The invention relates to the technical field of animal medicines, and particularly discloses a veterinary medicine compound preparation as well as a preparation method and application thereof. The veterinary medicine compound preparation is prepared by boiling, extracting, concentrating and diluting eight Tibetan medicines including aconitum pendulum, fructus chebulae, radix inulae inula, benzoin, pterocephalus hookeri, lagotis lagotis, amomum tsao-ko and rhodiola rosea. The veterinary drug compound preparation provided by the invention has an antibacterial range of 8.67 mm to 23.77 mm on enterotoxic large intestine dried bacteria, salmonella enteritidis and pasteurella multocida, and has an inhibition rate of 60% to 72% on enterotoxigenic escherichia coli diarrhea, salmonella enteritidis diarrhea and pasteurella multocida diarrhea; therefore, the veterinary drug compound preparation provided by the invention has a remarkable inhibition effect on enterotoxic large intestine dry bacteria, salmonella enteritidis and pasteurella multocida.

Lactobacillus plantarum strain LS2 and application thereof

Resumen de: CN120966673A

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LS2 and application thereof.The lactobacillus plantarum strain LS2 is separated from cheese, has the high GABA production capacity and the nitrite degradation capacity, has the antibacterial effect on enteric pathogenic bacteria including escherichia coli, salmonella enteritidis and campylobacter jejuni, and has the advantages that the lactobacillus plantarum strain LS2 is high in GABA production capacity and nitrite degradation capacity; the strain has good antibacterial function, good acid resistance and cholate resistance, and can well survive in artificial intestinal juice and artificial gastric juice; in order to improve the yield of GABA, the accelerant capable of remarkably promoting the growth of the strain LS2 is obtained by researching the accelerant, the highest survival rate of the freeze-dried powder prepared by optimizing the accelerant and the freeze-dried powder can reach 99% or above, the nitrite degradation rate can reach 98% or above, the yield of GABA is improved to 3.3 g/L or above, and the freeze-dried powder is bacterial powder capable of being widely applied.

アセトキシ水素化ホウ素類を用いた糖抗原のコンジュゲーション

Resumen de: CN120418260A

The present disclosure generally relates to improved methods for preparing glycoconjugates. The method includes conjugating a saccharide to a carrier protein using an acetoxyborohydride-containing reducing mixture prepared in situ.

Antibacterial peptide p-1-SB and application thereof

Resumen de: CN120943899A

The invention belongs to the technical field of antibacterial products, and relates to an antibacterial peptide p-1-SB and application thereof. The amino acid sequence of the antibacterial peptide p-1-SB is as shown in SEQ ID NO.1, the antibacterial peptide p-1-SB is screened from a probiotic fungus Saccharomyces boulardii, and the antibacterial peptide p-1-SB has the advantages of low hemolysis, low cytotoxicity, high stability and efficient in-vivo antibacterial activity, and has application safety. The antibacterial peptide p-1-SB has strong antibacterial activity on Gram-negative bacteria enterohemorrhagic escherichia coli, urinary tract infection escherichia coli, shigella, salmonella and acinetobacter baumannii which are commonly infected by a human body, and has potential application value in development of prevention and treatment of bacterial infections such as intestinal diarrhea or urinary tract infection.

Plant additive of piglet feed, piglet feed, piglet milk powder material and application

Resumen de: CN120937995A

The embodiment of the invention provides a plant additive of a piglet feed, the piglet feed, a piglet milk powder material and application. According to the plant additive for the piglet feed, antibacterial components of the plant additive are composed of eucommia ulmoides extract and orange peel extract, and the mass ratio of the eucommia ulmoides extract to the orange peel extract is (2.0-3.0): (1.0-1.5). By adopting the compatibility of the folium cortex eucommiae extract and the orange peel extract, the reproduction of coliform and salmonella as pathogenic bacteria in the intestinal tracts of the piglets can be effectively inhibited, and the plant additive has anti-inflammatory and antibacterial effects on the intestinal tracts of the piglets, so that the intestinal health of the piglets can be maintained, and the absorption of the piglets on nutrient substances of the feed after eating is ensured; the survival rate and the weaning weight of the piglets are favorably improved.

Salmonella colorimetric detection method based on DNAWalker driving double-signal amplification

Resumen de: CN120945079A

The invention develops a colorimetric detection method for salmonella based on DNAWalker driving double signal amplification. The detection principle is as follows: target DNA triggers AP hybridization to form Y-type connection with CP, an Nt.BbvCI restriction enzyme cutting site is exposed, DNAWalker is released through restriction enzyme cutting circulation, and target circulation amplification is realized. Meanwhile, PDANS (palladium-iron-nano particles) is synthesized through Fe < 2 + >/H2O2 Fenton reaction, a pH indicator is loaded through silver nano particle modification (PDANS (at) Ag), and a signal is enhanced. An amplified product is combined with PDANS (at) Ag through a magnetic bead sandwich structure, remarkable color change is caused, and quantitative detection can be performed through a smart phone App (such as' Color Grab ') or a microwell plate reader. The method is easy and convenient to operate, high in sensitivity and suitable for on-site rapid identification of salmonella.

Beef cattle feed additive and preparation method thereof

Resumen de: CN120937994A

The invention provides a beef cattle feed additive and a preparation method thereof, and belongs to the technical field of feed additives.Eucommia ulmoides fermentation extract is added to the feed additive, contains a large amount of chlorogenic acid and has a synergistic effect with citrus flavone, the content of fat in beef muscles is remarkably increased, nano-coated slow-release selenium yeast is added, the selenium bioavailability is increased, and the feed additive has the advantages of being capable of improving the beef quality and the like. Meanwhile, the citrus flavonoid microcapsules are added, so that the release of the citrus flavonoid microcapsules in rumen is reduced, the citrus flavonoid microcapsules are released in a targeted manner in the abomasum, and the utilization rate of citrus flavonoid is increased. The probiotics and the traditional Chinese medicine mixture are combined to inhibit escherichia coli and salmonella, and the illness probability of beef cattle is reduced. In the raw materials, through the synergistic effect of the composite mineral substances and the traditional Chinese medicine components, the anti-stress capability of the beef cattle is improved.

ENGINEERED IMMUNOSTIMULATORY BACTERIAL STRAINS AND USES THEREOF

Resumen de: US2025345369A1

Provided are delivery immunostimulatory bacteria that have enhanced colonization of tumors, the tumor microenvironment and/or tumor-resident immune cells, and enhanced anti-tumor activity. The immunostimulatory bacteria are modified by deletion of genes encoding the flagella, or by modification of the genes so that functional flagella are not produced, and/or are modified by deletion of pagP or modification of pagP to produce inactive PagP product. As a result, the immunostimulatory bacteria are flagellin and/or pagP−. The immunostimulatory bacteria optionally have additional genomic modifications so that the bacteria are adenosine or purine auxotrophs. The bacteria optionally are one or more of asd−, purI−, and msbB−. The immunostimulatory bacteria, such as Salmonella species, are modified to encode immunostimulatory proteins that confer anti-tumor activity in the tumor microenvironment, and/or are modified so that the bacteria preferentially infect immune cells in the tumor microenvironment, or tumor-resident immune cells, and/or are modified to induce less cell death in immune cells than in other cells. Also provided are methods of inhibiting the growth or reducing the volume of a solid tumor by administering the immunostimulatory bacteria.

DETECTION OF PATHOGENIC E. COLI STRAINS

Resumen de: WO2025233323A1

The invention relates to compositions of binding peptides, and individual binding peptides, capable of specific binding to either of two variants of OmpA occurring in pathogenic E. coli, and to methods employing these binding peptides for detection or isolation of pathogenic E. coli.

항-박테리아 단백질 복합체

Resumen de: MX2025009214A

The invention relates to protein bacteriocins (PBs) as therapeutic agents, and specifically to protein complexes comprising two or more PB molecules associated with a protein scaffold which comprises cognate immunity protein domains for the effector portions of the respective PBs. In particular, the invention provides an anti-bacterial protein complex comprising (a) a first PB molecule and a second PB molecule; and (b) an immunity protein scaffold comprising a first immunity protein domain and a second immunity protein domain; wherein the first and second immunity protein domains are non-covalently bound to the respective first and second PB molecules.

Chicken preservation method based on compound essential oil extracted from natural plants

Resumen de: CN120918228A

The invention provides a chicken preservation method based on compound essential oil extracted from natural plants. The preparation method comprises the following steps: selecting thyme leaves, clove buds and cortex cinnamomi as raw materials, and fermenting by utilizing lactobacillus plantarum and saccharomyces cerevisiae to obtain fermented essential oil; uniformly mixing and stirring the fermented essential oil, rosemary essential oil and tea tree essential oil to form compound essential oil; preparing a nano emulsion; clathration is performed to obtain microencapsulated fermentation essential oil; and uniformly spraying the microencapsulated essential oil emulsion on the surface of the chicken, performing vacuum packaging, and performing refrigeration at 4 DEG C. According to the invention, the plant essential oil is modified through microbial fermentation, and a nano slow-release technology is combined, so that an efficient, safe and lasting fresh-keeping effect is realized, the active ingredients of the compound essential oil are increased after fermentation, the inhibition effect on common food-borne pathogenic bacteria such as salmonella and staphylococcus aureus is remarkably improved, and the refrigeration and fresh-keeping period of chicken is prolonged to 21 days or more.

Antibacterial compound, preparation method and application thereof, and pesticide agent

Resumen de: CN120923334A

The invention relates to the technical field of natural antibacterial compounds, in particular to an antibacterial compound, a preparation method and application thereof and a pesticide agent. The antibacterial compound is selected from compounds shown in the following structural formula: the compound has an inhibition effect on methicillin-resistant staphylococcus aureus and other drug-resistant bacteria, staphylococcus aureus, escherichia coli, salmonella, pseudomonas aeruginosa and other bacteria, and alternaria, fusarium, colletotrichum and other fungi; the compound can be applied as broad-spectrum antibacterial, broad-spectrum antifungal and drug-resistant bacterium resistant drugs.

Microflora for treating industrial wastewater

Resumen de: CN120924423A

The invention discloses a flora for treating industrial wastewater and application of the flora for treating the industrial wastewater or polluted soil, the flora comprises stenotrophomonas and at least two selected from the group consisting of comamonas, ochrobactrum, pseudomonas, brucella, citrobacter, salmonella, balloon and enterococcus, and wherein the industrial wastewater contains a bactericide.

一种马链球菌和马流产沙门菌二联融合抗原及应用

Nº publicación: CN120924562A 11/11/2025

Solicitante:

新疆农业大学

Resumen de: CN120924562A

本发明公开了一种马链球菌和马流产沙门菌二联融合抗原，其核苷酸序列如SEQ ID NO.1所示。本发明将马流产沙门菌的FljB与马链球菌的SeM、EAG、GAPDH部分抗原串联重组在一起，不仅可以同时预防马链球菌和马流产沙门菌2种不同的马传染病，还增强了小鼠的体液免疫应答水平，利用该融合抗原免疫可获得抵抗马链球菌和马流产沙门菌的攻毒保护效果，高于单独抗原蛋白的免疫和灭活全菌的免疫，这解决了以往灭活疫苗免疫效率低和免疫繁琐的问题，有利于降低免疫的成本。