Alerta

BREAST MILK-DERIVED LIMOSILACTOBACILLUS FERMENTUM MSJK0025 CAPABLE OF IMPROVING INTESTINAL FLORA CONDITION AND USE THEREOF

Resumen de: EP4545628A1

The present application relates to a Limosilactobacillus fermentum MSJK0025 from breast milk for adjusting gut microbiota and an application thereof. The Limosilactobacillus fermentum MSJK0025 from breast milk is preserved in China General Microbiological Culture Collection Center, with a preservation number of CGMCC No.26426. The Limosilactobacillus fermentum from breast milk provided in the present application is a new strain separated from the healthy nursing woman, named as "Limosilactobacillus fermentum MSJK0025", having good antibiotics sensitivity, and its property can be stably inherited. It has good resistance to acid, bile salt, and artificial gastric juice and intestinal juice, and fast growth rate and good safety. At the same time, the strain has strong survival capability in the gut, and effectively inhibits pathogenic bacteria in the gut such as Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, and Candida albicans, but does not inhibit the probiotic in the gut such as lactobacillus and bifidobacterium, so the strain has broad application prospect in adjusting the balance of gut microbiota.

基于碳水化合物的抗沙门氏菌属疫苗的研发

Resumen de: AU2023312737A1

Provided herein are vaccine composition comprising a

TUMOR NEOANTIGEN VACCINE SYSTEM SPECIFICALLY MARKING HETEROLOGOUS PROTEIN, AND USE THEREOF

Resumen de: WO2025081515A1

The present invention provides a tumor neoantigen vaccine system specifically marking a heterologous protein, and a use thereof. The vaccine system is composed of a heterologous protein mRNA vaccine and a recombinant oncolytic virus; the heterologous protein mRNA vaccine is used to induce an organism to generate heterologous protein-specific memory T cells, and the recombinant oncolytic virus is used to promote a tumor to express microorganism-derived tumor neoantigens. When pre-immunized memory T cells detect these marked specific neoantigens, the tumor expressing the neoantigens is quickly activated and killed, the release of tumor autoantigens is promoted, dendritic cells phagocytize antigens to further initiate an anti-tumor response, and a systemic anti-tumor immune effect is activated by means of antigenic epitope spreading.

IMMUNOSTIMULATORY COMPOSITION

Resumen de: US2025127889A1

The present disclosure relates to a composition for inducing migration of activated B cells to a germinal center, the composition including a substance that induces CD11b expression in B cells, a production method therefor, or use thereof. In one aspect, the present disclosure relates to immunostimulation and maintenance or improvement of mucosal flora using the substance that induces the CD11b expression in B cells.

METHOD FOR PREPARING BIOFLOC CULTURE MEDIUM BY USING ORGANIC WASTES

Resumen de: WO2025081938A1

Provided is a method for preparing a biofloc culture medium by using organic wastes. The method comprises: adding a first auxiliary material to organic wastes, uniformly mixing same to obtain a mixture, and adjusting the water content of the mixture to 45-55%; inoculating an activated Bacillus subtilis seed culture, Bacillus licheniformis seed culture and Saccharomyces cerevisiae seed culture into the mixture according to a ratio, and performing fermentation with stirring and ventilation, so as to obtain a fermented product; adding a second auxiliary material to the fermented product, and uniformly mixing same to obtain the biofloc culture medium. In the finished biofloc product prepared by the method, the viable count of Bacillus subtilis is greater than or equal to 400 million cfu/g, the viable count of Bacillus licheniformis is greater than or equal to 200 million cfu/g, the viable count of Saccharomyces cerevisiae is greater than or equal to 1200 million cfu/g, the total number of bacterial colonies is greater than or equal to 8000 million cfu/g, and no salmonella is detected. The present application can solve the pollution problem of livestock and poultry manure and further achieves recycling utilization of livestock and poultry manure.

標的微生物を検出するための反応媒体、及び関連する方法

Resumen de: AU2023262222A1

The invention relates to a gelled reaction medium for the detection, identification, enumeration and/or isolation of at least one target microorganism in a sample that may contain same, comprising at least one binding partner specific to a component of a target microorganism or of a component derived from said microorganism, coupled to at least one nanoparticle to form at least one binding conjugate.

鼠伤寒沙门菌毒力基因Ict的鉴定及其减毒疫苗候选株的制备与应用

Resumen de: CN119842745A

本发明公开了鼠伤寒沙门菌毒力基因Ict的鉴定及其减毒疫苗候选株的制备与应用。本发明选择强毒力鼠伤寒沙门菌SL1344株作为亲本株，将其中降解代谢衣康酸的关键毒力基因Ict进行敲除，构建鼠伤寒沙门菌SL1344减毒疫苗候选株。通过小鼠动物毒力实验、免疫保护力实验和安全性实验等证实该疫苗候选株SL1344‑ΔIct缺失株，不仅对动物的致病力显著降低，且提供了良好的免疫原性和免疫保护力。本发明实现对强毒力鼠伤寒沙门氏菌株的减毒，可广泛应用于沙门菌的防控，为研制沙门菌的减毒活疫苗及活载体疫苗奠定基础。

Duplex fluorescent PCR detection primer probe group for duck salmonella enteritidis and salmonella typhimurium, kit, and use thereof

Resumen de: GB2634693A

A duplex fluorescent PCR detection primer probe group of duck Salmonella enteritidis and Salmonella typhimurium, a kit, and the use thereof. Comparative analysis on the whole genomes of Salmonella enteritidis and Salmonella typhimurium is performed to screen out as identification targets a specific fragment Sdf I of lyg D gene of Salmonella enteritidis and a specific gene STM4497 of Salmonella typhimurium, and the present application designs specific primers and probes.

Rapid diagnosing Composition for detecting multiple Salmonella enterica serovars

Resumen de: KR20250049691A

본 발명은 살모넬라 혈청형 동시 검출을 위한 신속 진단 조성물에 관한 것으로서, 본 발명에서는 인간에서 발병률이 높은 여러 살모넬라 엔테리카(Salmonella enterica) 혈청형을 진단하기 위한 신속 도트-블롯 면역분석법을 개발하였다. 10개의 상업용 항체(4개의 다클론 항체 및 6개의 단클론 항체)에 대한 적용 가능성을 2016년 미국에서 67.5%의 살모넬라 감염 사례와 관련된 18개의 혈청형을 사용하여 시험하였다. Ab 3(다클론; 18개 혈청형 중 8개), Ab 8(단클론; 18개 혈청형 중 13개) 및 Ab 9(단클론; 18개 혈청형 중 10개) 항체는 웨스턴 블롯에서 높은 검출율을 나타냈고, 두 항체의 조합(Ab 3+8, Ab 3+9 및 Ab 8+9)을 도트-블롯 분석에 적용하였다. Ab 3+8의 조합은 시험된 18개의 혈청형 중 15개를 식별할 수 있었는데, 이는 S. Enteritidis, S. Typhimurium, S. Javiana, S. I 4,5,12:i:-, S. Infantis, S. Montevideo, S. Braenderup, S. Thompson, S. Saintpaul, S. Heidelberg, S. Oranienburg, S. Bareilly, S. Berta, S. Agona 및 S. Anatum을 포함하며, 2016년 미국에서 53.7%의 살모넬라 감염 사례와 관련되어 있다. 본 발명에서 최적화한 면역분석법은 3시간 안에 주요 15종의 혈청형을 진단할 수 있고, 이러한 비용 효율적이고 신속한 방법은 살모넬라 검출을 위한 현장 비색 방법으로 유용하게 활용될 수 있을 것으�

一种减毒沙门氏菌来源的细菌外膜囊泡蛋白载药系统及其制备方法和应用

Resumen de: CN119792228A

本发明公开了一种减毒沙门氏菌来源的细菌外膜囊泡蛋白载药系统及其制备方法和应用，本发明利用细菌外膜囊泡蛋白载药系统可以在细菌外膜囊泡表面实现蛋白药物的“即插即用”。本发明构建了细菌外膜囊泡蛋白载药系统。本发明敲除了减毒鼠伤寒沙门氏菌VNP20009的OmpA，并回补在胞外环结构域中插入SpyTag的重组OmpA，利用SpyTag/SpyCatcher间自发形成异肽键的性质，将SpyCatcher与蛋白药物的融合蛋白展示在细菌外膜囊泡表面，制备方法简单，容易操作，在细菌外膜囊泡表面展示多种蛋白，并高效保护和负载蛋白药物。结合细菌外膜囊泡的免疫刺激特性，实现肿瘤的高效治疗。

SipD重组蛋白在制备检测禽沙门氏菌抗体的间接ELISA检测试剂盒中的应用

Resumen de: CN119804862A

本发明涉及血清学检测技术领域，特别是涉及SipD重组蛋白在制备检测禽沙门氏菌抗体的间接ELISA检测试剂盒中的应用。本发明通过大量研究，成功筛选出用于检测禽沙门氏菌抗体的特异性抗原‑SipD蛋白。本发明以禽沙门氏菌III型分泌系统SipD亚基(SipD蛋白)为抗原，经蛋白表达纯化后获得包被抗原(SipD重组蛋白)，通过反应条件的摸索与优化，最终建立了灵敏性高、特异性强和重复性好的禽沙门氏菌间接ELISA检测方法。相比于传统的玻片凝集抗原检测方法，该方法极大地提高了检测通量和检测效率。该检测方法的建立对于人类、动物、食品中的沙门氏菌检测具有重要的公共卫生意义。

IMPROVED SALMONELLA VECTORED THERAPIES FOR TREATMENT OF CANCER

Resumen de: WO2023235633A2

A genetically modified Salmonella cell (GMSC) engineered to exhibit specific targeting to cells and regulated delayed lysis in vivo, the GMSC comprising a first heterologous nucleic acid that encodes a first gene product that causes the GMSC to be selectively localized to and/or internalized by a target cell in vivo and a second heterologous nucleic acid that encodes a second gene product that facilitates killing of the target cells following internalization.

METHOD FOR IDENTIFYING ESBL ENZYMES IN ENTEROBACTERIA

Resumen de: WO2023227848A1

The present invention relates to a method for determining the properties of resistance to beta-lactam antibiotics of a strain of enterobacteria present in a biological sample, which method comprises the steps of: a) lysis of the bacteria and hydrolysis of the bacterial proteins in order to obtain a mixture of peptides, b) analysis of this mixture of peptides by targeted mass spectrometry coupled with reverse phase liquid chromatography, detection of at least one specific peptide of an enzyme exhibiting extended spectrum beta-lactamase (ESBL) activity being indicative of the resistance to beta-lactams of the strain of enterobacteria present in the biological sample, the method being characterised in that the analysis step (b) is carried out in the presence of an ion pairing reagent (IPR) added into the chromatography column or into the mixture of peptides.

一种感应铁镁离子的细菌传感器的构建方法及其应用

Resumen de: CN119776251A

本发明公开了一种感应铁镁离子的细菌传感器的构建方法及其应用。本发明属于微生物领域，具体涉及一种感应铁镁离子的细菌传感器的构建方法及其应用。重组大肠杆菌含有超折叠绿色荧光蛋白sfGFP编码基因、感应蛋白PmrB突变体编码基因和响应调节蛋白PmrA突变体编码基因、信号传导蛋白PmrD编码基因和强核糖体结合位点RBS，并含有通过调节蛋白PmrA激活的磷酸化酶PmrG的启动子驱动的T7 RNA聚合酶编码基因和T7启动子驱动的sfGFP编码基因，同时过表达磷酸乙醇胺转移酶EptA编码基因。本发明构建了检测铁离子浓度的细菌传感器，并可通过铁镁离子的诱导改变细菌传感器的脂多糖的修饰，增强细菌的耐药性。

一株马流产沙门菌crp基因缺失株及其构建方法和作为减毒疫苗候选株的应用

Resumen de: CN119752748A

本发明属于兽医生物制品领域，具体涉及一株驴源马流产沙门菌crp基因缺失株YZUSAL01Δcrp及其构建方法和应用。本发明的基因缺失株已保藏于中国典型培养物保藏中心，保藏号为CCTCC NO:M 20241741。该缺失株是利用λ‑Red同源重组技术，将马流产沙门菌的crp基因敲除后获得的。本发明制备的基因缺失株毒力显著下降，具有良好的安全性。对小鼠的攻毒免疫保护效果良好，为马流产沙门菌减毒活疫苗奠定基础。

CRISPR-CAS-BASED COMPOSITION FOR SALMONELLA DETECTION AND SALMONELLA DETECTION METHOD USING SAME

Resumen de: US2025109447A1

The present invention relates to a CRISPR-Cas based composition for detecting Salmonella and a method for detecting Salmonella using the same, more specifically, to a composition for detecting Salmonella comprising primers capable of specifically amplifying Salmonella through isothermal amplification, guide RNA, and CRISPR-Cas proteins, and a method for detecting Salmonella using the same.

METHOD FOR DETECTING BIOLOGICAL OBJECTS BY MEANS OF SURFACE PLASMON RESONANCE IMAGING

Resumen de: US2025110128A1

A method for detecting biological objects by means of an SPR imaging detection system comprising an optical measurement device configured to generate plasmon resonance on a functionalized surface when said surface is exposed to a gas; the method comprising: an assimilation step by exposing the functionalized surface to a sample of interest formed of an aqueous carrier liquid containing the biological objects; a step of removing the liquid in contact with the functionalized surface and exposing the surface to a gas not containing the biological objects, said objects remaining bound to the ligands of the sensitive site of the functionalized surface; a step of acquiring an image of the sensitive site; a step of detecting the biological objects from the acquired image.

계절성 및 신종 감염의 치료 및 예방을 위한 백신

Resumen de: AU2023323947A1

The invention is directed to immunogenic compositions and method of treatment comprising a peptide or nucleic acid that encodes the peptide that induces an immune response in a mammal that is protective against infection by one or more pathogens. The peptide sequence contains multiple epitopes, wherein at least one epitope is a composite epitope which is a combination of two or more conserved epitopes of the pathogen wherein the amino acid sequence of the composite is not an amino acid sequence of the pathogen. In addition, the invention is directed to vaccines comprising the peptide or nucleic acid that encodes the peptide for treating and preventing an infection in mammals such as animals and humans.

一种表达Bcf菌毛的重组菌株及其应用

Resumen de: CN119736221A

本发明公开了一种表达Bcf菌毛的重组菌株，重组菌株由将含有Bcf操纵子基因的表达载体导入惰性载体菌中得到。将Bcf操纵子基因插入到表达载体pBR322中得到重组质粒，将所述重组质粒导入惰性载体菌S9中得到所述重组菌株，惰性载体菌S9的保藏编号为CGMCC No.17340。上述重组菌株在快速、特异性、敏感性、稳定性、简单和价廉等方面表现更好，能够满足畜禽群的沙门氏菌感染现场和大规模、大批量、快速检测的要求，通过靶向检测多种血清型沙门氏菌感染，适用于沙门氏菌的大规模初筛工作。还能够对沙门氏菌疫苗接种后抗体水平进行监测。

Novel bacteriophage with specific killing ability against Salmonella enterica and antibacterial composition containing the same

Resumen de: WO2025063348A1

The present invention relates to a novel bacteriophage having specific killing ability against Salmonella enterica and an antibacterial composition comprising same, wherein the composition comprising the bacteriophage as an active ingredient can be usefully employed as a composition for preventing or treating infectious diseases caused by Salmonella enterica, feed for livestock, drinking water, a cleaning agent, and a disinfectant.

一株沙门氏菌噬菌体PC192、其噬菌体组合物及其应用

Resumen de: CN116121203A

The invention discloses a clostridium welchii bacteriophage PM11, a bacteriophage composition and application of the clostridium welchii bacteriophage PM11, the clostridium welchii bacteriophage PM11 is used for preventing and controlling poultry bacterial diarrhea, the preservation number of the clostridium welchii bacteriophage PM11 is CGMCC No.22372, and the clostridium welchii bacteriophage PM11 is preserved in the China General Microbiological Culture Collection Center on April 12, 2021 and has the preservation number of CGMCC The invention also discloses a bacteriophage composition formed by compounding the clostridium welchii bacteriophage PM11, an escherichia coli bacteriophage PD205 and a salmonella bacteriophage PC192, and the clostridium welchii bacteriophage and the bacteriophage composition can be used as active ingredients for effectively preventing and treating avian bacterial diarrhea, and can also be used as an environment disinfectant. And compared with a single lytic bacteriophage, the bacteriophage has the characteristic of wide lysis spectrum, widens the sterilization range and improves the sterilization activity.

PROBIOTIC AND PREBIOTIC COMPOSITION AND USE THEREOF

Resumen de: WO2025060396A1

A probiotic and prebiotic composition and the use thereof. The composition comprises human milk oligosaccharides, Bifidobacterium bifidum and Bifidobacterium longum subsp. infantis, and has the advantages of improving the resistance of gastrointestinal tracts to pathogenic bacterial infection and reducing colonization of Salmonella.

SALMONELLA STRAIN WITH CHROMOSOMALLY INTEGRATED LANDING PAD

Resumen de: AU2023346467A1

The present invention relates to a modified live attenuated strain of Salmonella, said strain comprising at least one chromosomally integrated synthetic polynucleotide sequence inserted into a pre-determined pseudogenomic location, said sequence comprising at least one defined recombination site for the introduction of a heterologous polynucleotide sequence encoding a polypeptide, wherein said chromosomally integrated synthetic polynucleotide sequence is located within at least one genomic locus as defined by any one of SEQ ID NOs: 1-30, or a sequence comprising at least 70% identity to any one of SEQ ID NOs: 1-30. The present invention also relates to a vaccine composition comprising the modified strain and various uses and methods thereof.

A Novel Poultry Salmonella Vaccine and Diagnostic Methodology to Control Foodborne Salmonellosis

Resumen de: US2025099564A1

A vaccine for treating Salmonella enteriditis that includes an immunogenically effective amount of a Salmonella enteritidis protein InvG, and optionally a pharmaceutically acceptable carrier is described. Methods of using compositions that include the Salmonella Enteritidis protein InvG or a delivery vector that expresses Salmonella Enteritidis protein InvG for immunizing poultry against Salmonella Enteritidis are also described.

NOVEL BACTERIOPHAGE HAVING SPECIFIC KILLING ABILITY AGAINST SALMONELLA ENTERICA AND ANTIBACTERIAL COMPOSITION COMPRISING SAME

Nº publicación: WO2025063348A1 27/03/2025

Solicitante:

SHLAB INC [KR]
(\u110C\u116E)\uC5D0\uC2A4\uC5D0\uC774\uCE58\uB7A9

Resumen de: WO2025063348A1

The present invention relates to a novel bacteriophage having specific killing ability against Salmonella enterica and an antibacterial composition comprising same, wherein the composition comprising the bacteriophage as an active ingredient can be usefully employed as a composition for preventing or treating infectious diseases caused by Salmonella enterica, feed for livestock, drinking water, a cleaning agent, and a disinfectant.