Alerta

Construction method of novel coronavirus-like particle induced cell membrane fusion model

Resumen de: CN122427960A

The invention discloses a construction method of a novel coronavirus-like particle induced cell membrane fusion model, and belongs to the technical field of biology, and the method comprises the following steps: constructing virus-like particles containing SARS-CoV-2 spike protein genes and RNA packaging sequence PS9, taking HEK-293T cells as packaging cells, co-expressing structural proteins M, E, N and S, and realizing VLPs assembly without replication ability. And then, infecting immortalized II-type alveolar epithelial cells by using the VLPs, and inducing S protein expression and mediating cell-cell membrane fusion under the condition of not needing a BSL-3 laboratory. The formation of fused giant cells can be visually observed through ZO-1 protein immunofluorescence staining, and the model is stable, high in biological safety and repeatable in vitro. The method overcomes the problems of safety risk of live virus infection and insufficient authenticity of a transient transfection model, can be used for research of an SARS-CoV-2 virus membrane fusion mechanism, and has a good application prospect.

Isothermal-PfAgo-RDB-based nucleic acid detection system, kit and detection method

Resumen de: CN122428060A

The invention relates to the technical field of biological detection, in particular to a nucleic acid detection system based on isothermal-PfAgo-RDB, a kit and a detection method, a primer group comprises F3, B3, FIP, BIP, LF and LB; the nucleic acid detection system comprises a nucleic acid isothermal amplification module, a PfAgo cutting module and an RDB visualization module; the target comprises an SARS-COV-2 O gene and/or an SARS-COV-2 N gene and/or MP (Methyl Pyrrolidone) and/or PIVs (Phosphatidylcholine); the PfAgo cutting module comprises gDNA (deoxyribonucleic acid), PfAgo and a probe; the probe comprises a fluorescent probe and an identification probe; a nucleic acid detection method based on isothermal-PfAgo-RDB comprises the following steps: (1) isothermal amplification: designing primers for target nucleic acid SARS-COV-2 O gene and/or SARS-COV-2 N gene and/or MP and/or PIVs, and carrying out isothermal amplification; (2) PfAgo cutting: guiding PfAgo to carry out specific cutting on a target in the sample through the 5 '-phosphorylated gDNA, so as to obtain a PfAgo treating fluid; and (3) carrying out RDB hybridization and color development. The kit has the advantages of high sensitivity, strong specificity, no cross reaction, high flux, low cost, suitability for mixed infection screening and the like.

Treatment and prevention of hepatitis b using covid-19 vaccine

Resumen de: CN122421982A

Treatment and prevention of hepatitis B are provided. In particular, a method for treating or preventing hepatitis B in a subject is provided, the method comprising administering to the subject an effective amount of a COVID-19 vaccine. Further provided is the use of a COVID-19 vaccine in the manufacture of a medicament for the treatment or prevention of hepatitis B.

Application of cytidine derivative in preparation of medicine for preventing and/or treating Ebola virus infection

Resumen de: CN122398841A

The invention provides application of a cytidine derivative or a pharmaceutically acceptable salt thereof in preparation of a medicine for preventing and/or treating Ebola virus infection. The structural formula of the cytidine derivative is shown as a formula (I) in the specification. According to the invention, the new potential of'old medicine 'is excavated, and the oral medicine, namely, alzovudine (FNC), which is originally developed for treating human immunodeficiency virus and new coronavirus infection is used for treating the Ebola virus. At present, the death rate caused by the Ebola virus is up to 90%, and no effective treatment medicine is available for the disease at present. Therefore, research on drugs for the Ebola virus is extremely urgent. Experimental results show that the FNC has good Ebola virus inhibition activity and low cytotoxicity, and the Ebola virus inhibition activity of the FNC is significantly superior to that of HIV and new coronavirus. According to the invention, old medicines are newly used, a more direct solution is provided for Ebola virus treatment, and an important role is played in epidemic situation control. (I)

Peptidomimetic compound or derivative thereof and application thereof

Nº publicación: CN122404474A 17/07/2026

Solicitante:

NOBEL INST OF BIOMEDICINE CO LTD
THE UNIV OF HONG KONG
CENTRO DE INVESTIG Y VACCINE SRL
\u73E0\u6D77\u8BFA\u8D1D\u5C14\u56FD\u9645\u751F\u7269\u533B\u836F\u7814\u7A76\u9662\u6709\u9650\u516C\u53F8
\u9999\u6E2F\u5927\u5B66
\u75C5\u6BD2\u4E0E\u75AB\u82D7\u7814\u7A76\u4E2D\u5FC3\u6709\u9650\u516C\u53F8

Resumen de: CN122404474A

The invention relates to peptidomimetic compounds or derivatives thereof and applications thereof. The invention provides a peptidomimetic compound and a derivative thereof, a compound shown in a formula (I), or an isomer, a pharmaceutically acceptable salt or a prodrug thereof. The compound and the derivative of the compound can effectively inhibit 3CL protease, can be prepared into drugs for treating and/or preventing SARS-CoV, SARS-CoV-2, MERS, HBV or HCV virus infection diseases, are novel in chemical structure and have good biological activity.