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一种抗大肠埃希氏菌的纳米抗体及其制备方法和应用

Resumen de: CN122562945A

本发明提供了一种抗大肠埃希氏菌的纳米抗体及其制备方法和应用，属于分子生物学、噬菌体展示技术以及蛋白质组学技术领域。所述抗大肠埃希氏菌的纳米抗体具有与大肠埃希氏菌特异性结合的功能，本发明公布了该纳米抗体及编码该纳米抗体的基因序列，该纳米抗体的表达载体和宿主细胞以及生产该纳米抗体的方法。本发明提供的抗大肠埃希氏菌的纳米抗体具有体积小、表达效率高、溶解性好、稳定性强等优点，在食品中大肠埃希氏菌检测方面具有良好的应用前景。

一种肿瘤靶向定植且肠道可条件性清除的工程菌及其应用

Resumen de: CN122563840A

本发明公开了一种肿瘤靶向定植且肠道可条件性清除的工程菌及其应用。该工程菌首次整合了“肿瘤专性定植回路”与“大肠杆菌素条件性敏感回路”：通过营养缺陷型突变（ΔaroA）与缺氧依赖性增殖回路（P hypox ‑ftsZ）的双重锁定，实现严格的肿瘤微环境专性定植；通过外源大肠杆菌素受体基因与时间延迟免疫蛋白回路的协同，实现在肠道穿越窗口期（约0‑4小时）内对大肠杆菌素耐受、穿越完成后恢复敏感的条件性清除能力。本发明工程菌解决了口服活菌制剂“既要高效穿越肠屏障、又要彻底清除肠道残留”的核心矛盾，为肿瘤细菌疗法提供了安全、可控的活体底盘，可广泛用作定植菌或效应菌应用于各类级联放大肿瘤免疫治疗系统。

NOVEL COMPOUND

Resumen de: WO2026170052A1

The present invention provides for enrichment of animal foodstuffs using endolysin polypeptides or fragments thereof, which possess antimicrobial activity (e.g., against gram-negative bacteria, such as Salmonella enterica). The invention also provides for nucleic acid molecules encoding the endolysin, and methods of their production. Also provided are compositions comprising the endolysins, or comprising any of the means for producing the endolysin (e.g., a host cell, lysate, or dried product, etc.) that has antimicrobial activity, for the use of said compositions in a method of reducing the population of gram-negative bacteria in a foodstuff, or preventing or treating a disease or disorder in an animal.

RESPIRATORY DRY POWDER DELIVERY

Resumen de: US20260232581A1

0000 The present disclosure provides pharmaceutical compositions for use in delivering therapeutics, such as vaccines or biologic material, to the nasal cavity or the deep lungs. In particular, the pharmaceutical compositions may preferentially deposit on the lower or middle turbinate region and the nasopharynx region of the nasal cavity. In other aspects the pharmaceutical compositions may be used to generate powders that penetrate the deep lungs. Also, provided herein are methods of preferential delivery to these regions.

FLUID TREATMENT APPARATUS WITH PULSED ELECTRIC FIELDS

Resumen de: EP4790026A1

0001 Apparatus for treating various fluids, such as wine, must, water, oil, sauce, fruit juice, and others, to prevent contamination or the development and spread of diseases such as salmonella and legionella. The apparatus allows to avoid excessive proliferation of colonies of bacteria and yeasts which, if present in excessive quantities, can cause mould and pathogens in general. The apparatus comprises a container body containing a reticular cathode and anode that allow the liquid to pass through and are powered by an electrical connector that receives electrical current from a power supply controlled by an electronic control unit. The container body is configured to be immersed in the liquid to be treated, allowing the liquid to flow through the cathode and anode, which generate a pulsed electric field. The electrical signal has a frequency of about 300 Hz, an amplitude of 100V and pulses of few milliseconds. To increase the efficiency of the treatment, the signal changes polarity every 90 seconds.

免疫刺激細菌ベースのワクチン、治療薬およびＲＮＡデリバリープラットフォーム

Resumen de: WO2022036159A2

Provided are attenuated immunostimulatory bacteria with genomes that are modified to, for example, reduce toxicity and improve the anti-tumor activity, such as by increasing accumulation in the tumor microenvironment, particularly in tumor- resident myeloid cells, improving resistance to complement inactivation, reducing immune cell death, promoting adaptive immunity, and enhancing T-cell function. Also provided are immunostimulatory bacteria for use as vaccines, and for delivery of mRNA. The increase in colonization of phagocytic cells improves the delivery of encoded therapeutic products to the tumor microenvironment and to tumors and permits, among other routes, systemic administration of the immunostimulatory bacteria. The increase in colonization of phagocytic cells also provides for use of immunostimulatory bacteria for direct tissue administration for use as vaccines.

基于磁性MIPs与ZIF-8双识别的肺炎克雷伯菌分子印迹传感器的构建

Resumen de: CN122545801A

0001 本发明构建了一种基于磁性分子印迹与硼酸亲和协同作用的三明治结构传感器，用于肺炎克雷伯菌的快速可视化检测。该传感器以表面接枝双键的Fe3O4@SiO2为载体，以肺炎克雷伯菌为模板，通过自由基聚合制备了具有特异性识别空腔的磁性分子印迹聚合物（MIPs）。同时，合成了负载姜黄素的ZIF‑8材料，并修饰对羧基苯硼酸构建ZIF‑8@Cur@PBA信号探针，利用硼酸基团与细菌表面糖蛋白的顺式二醇基发生特异性结合。当目标菌存在时，MIPs与探针通过细菌介导形成三明治夹心结构，经磁分离后加入盐酸裂解ZIF‑8释放姜黄素，实现信号放大，再用氢氧化钠中和恢复颜色，通过肉眼观察实现可视化半定量检测，同时测定紫外吸光度进行定量分析。该传感器表现出优异的选择性、抗干扰能力和稳定性，为肺炎克雷伯菌的快速、灵敏检测提供了新方法，具有重要的实际应用价值。

一种基于微流控和银沉积的食源性致病菌的检测方法

Resumen de: CN122545614A

本发明公开了一种基于微流控和银沉积的食源性致病菌的检测方法，利用噬菌体功能化磁珠特异性捕获目标致病菌,通过高碘酸钠氧化细菌表面多糖生成醛基,诱导银氨溶液中Ag+原位还原为银单质并负载于细菌表面,再利用微流控芯片，结合Ag/AgCl阳极溶出伏安法检测氧化峰电流,实现致病菌的定量检测。本发明利用细菌表面多糖诱导原位银沉积实现信号放大,单个细菌表面可沉积大量银单质结合固态Ag/AgCl阳极溶出伏安法的高信噪比,检测灵敏度高,且在宽浓度范围内电流信号与致病菌对数浓度呈良好线性关系，且检测时间≤1小时,试剂消耗少,且试剂盒便携,适用于食品生产、流通、检测等场景的现场快速检测。

免疫原性組成物

Resumen de: WO2025021695A1

The present invention relates to immunogenic compositions comprising outer membrane vesicles (OMVs) adsorbed to an aluminium hydroxide adjuvant, whereby the aluminium hydroxide adjuvant is quenched. The present invention also relates to the use of the immunogenic compositions for providing protection against diseases caused by Gram-negative bacteria. The present invention also relates to a method for reducing particle aggregation and/or average particle diameter in compositions comprising OMVs and an aluminium hydroxide adjuvant.

一株杂交瘤细胞株、其分泌的沙门菌SifA蛋白单克隆抗体及应用

Resumen de: CN122521592A

本发明公开了一株杂交瘤细胞株、其分泌的沙门菌SifA蛋白单克隆抗体及应用。本发明所提供的单克隆抗体具有效价高、特异性强的优势。本发明采用基于沙门菌SifA蛋白单抗建立的间接竞争ELISA检测试剂盒在用于检测时可快速准确地检测出感染沙门菌的血清样品，与现有技术相比，缩短了检测时间、简化了检测步骤、拥有高通量及广谱性的优势，为快速、高效检测多种不同血清型沙门菌感染提供了可靠的免疫学技术。

一种可分泌微菌素MccY的减毒鸡白痢沙门菌及其制备方法和应用

Resumen de: CN122521544A

本发明公开了一种可分泌微菌素MccY的减毒鸡白痢沙门菌（pMccY‑asd），其制备方法及应用，归属于禽沙门菌病的防治领域。本发明提供的鸡白痢减毒沙门菌毒力显著降低，能有效清除强力菌株感染，并可应用平板凝集试验对免疫接种鸡和自然感染鸡予以区分。通过宿主载体平衡致死系统构建pMccY‑asd质粒，可以使菌株在不依赖抗生素筛选的情况下锁定质粒，并且持续高效地分泌表达MccY，对野生型鸡白痢沙门菌及不同血清型沙门菌有较好的抑菌作用。

一种沙门菌属特异性抗原片段、基因、试剂盒及其在检测鸡血清中沙门菌抗体中的应用

Resumen de: CN122520725A

本发明公开了一种沙门菌属特异性抗原片段、基因、试剂盒及其在检测鸡血清中沙门菌抗体中的应用。所述沙门菌属特异性抗原PagN片段的氨基酸序列如SEQ ID NO.1所示。本发明首次公开了沙门菌属特异性抗原PagN片段在沙门菌鸡群感染的血清学诊断价值，并将其作为间接ELISA的包被抗原，建立了沙门菌抗体间接ELISA检测方法。沙门菌外膜蛋白PagN片段能够特异性识别沙门菌属抗体，能绕过血清型差异造成的检测障碍，迅速锁定沙门菌属感染，适用于临床中需要快速初筛的场景。本发明能够用于鸡群中沙门菌属抗体的检测，为沙门菌的流行病学调查以及沙门菌感染的精准防控提供了有力的技术支持。

一种抗福氏志贺氏菌的纳米抗体及其制备方法和应用

Resumen de: CN122520769A

本发明提供了一种抗福氏志贺氏菌的纳米抗体及其制备方法和应用，属于分子生物学、噬菌体展示技术以及蛋白质组学技术领域。所述抗福氏志贺氏菌的纳米抗体具有与福氏志贺氏菌特异性结合的功能，本发明公布了该纳米抗体及编码该纳米抗体的基因序列，该纳米抗体的表达载体和宿主细胞以及生产该纳米抗体的方法。本发明提供的抗福氏志贺氏菌的纳米抗体具有体积小、表达效率高、溶解性好、稳定性强等优点，在食品中福氏志贺氏菌检测方面具有良好的应用前景。

大肠埃希氏杆菌感染的噬菌体疗法

Resumen de: US2017000831A1

0001 The present invention relates to bacteriophage therapy. More particularly, the present invention relates to novel bacteriophages having a high specificity against Escherichia coli strains, their manufacture, components thereof, compositions comprising the same and the uses thereof in phage therapy.

一种同时检测李斯特菌和大肠杆菌O157:H7的双探针体系、试纸条及检测方法

Resumen de: CN122525123A

0001 本发明公开了一种同时检测李斯特菌和大肠杆菌O157:H7的双探针体系、试纸条及检测方法，其中，构建的双探针体系包含AIENPs‑FAM荧光纳米探针和AIENPs‑地高辛荧光纳米探针，以及与其对应的免疫层析试纸条，进一步，构建的基于AIENPs的RPA‑CRISPR‑LFCS方法，可以同时实现对李斯特菌LM和大肠杆菌O157:H7的高灵敏筛查，LM的检出限为29 fg/μL（223 CFU/mL），大肠杆菌O157:H7的检出限为25.1 fg/μL（195 CFU/mL）。

COMPOSITIONS AND METHODS FOR ENHANCING ANIMAL FEED SAFETY AND CONTROLLING PATHOGENS

Resumen de: WO2026165105A1

The present inventive concept provides non-toxic compositions that include components selected from ethanol, polymers, polar aprotic solvents, water, organic acids, phospholipids, polyols, polysaccharides, phenols, plant extracts, organic salts, peroxide, and antibiotics. The compositions are designed as an enhancement or additive to animal feed additives, animal feedstuff, animal feed, or animal hydration sources. The compositions provide a safer alternative to traditional formaldehyde (e.g., aldehyde-based) compositions by incorporating preservative and antimicrobial agents into animal feed products. The compositions provided herein render animal feed highly resistant to recontamination by pathogenic bacteria such as Salmonella, Campylobacter, Streptococcus, Staphylococcus, E. coli, Clostridia, and Bacillus. Moreover, the compositions provide safer working conditions by eliminating workers' exposure to carcinogenic substances.

IN OVO VACCINATION OF GENETICALLY MODIFIED SALMONELLA VECTORED VACCINES

Resumen de: WO2026165563A1

Disclosed are methods for inoculating embryonated avian eggs for conferring protective immune responses against diseases caused by a diversity of bacterial, viral and parasite pathogens. According to certain aspects methods are disclosed that involve administering, in ovo, a safe inoculating dose of a Protective Immunity Enhanced Salmonella Vaccine (PIESV) strain, or derivative thereof, and optionally co-administering a safe inoculating dose of a live self-destructing attenuated adjuvant Salmonella strain (SDAAS), or derivative thereof.

COMPOSITIONS AND METHODS FOR CONTROLLING PATHOGENS AND IMPROVING HYGIENE IN THE POULTRY AND LIVESTOCK INDUSTRIES

Resumen de: WO2026165108A1

The present inventive concept provides methods using non-toxic compositions that include components selected from ethanol, polymers, polar aprotic solvents, water, organic acids, phospholipids, polyols, polysaccharides, phenols, plant extracts, organic salts, peroxide, and peptide antibiotics designed as hygienic agents for the poultry and livestock industries. The compositions and methods provide a safer alternative to use of traditional formaldehyde (e.g., aldehyde-based) compositions by incorporating non-toxic preservation and antimicrobial agents to enhance biosecurity and sanitation in the livestock environments, and in particular, poultry production environments. The methods provided herein also control the growth of pathogenic bacteria such as Salmonella, Campylobacter, Streptococcus, Staphylococcus, E. coli, Clostridia, and Bacillus. Moreover, the methods and compositions provide safer working conditions by eliminating workers' exposure to carcinogenic substances.

METHODS FOR PRODUCING HIGH-LEVEL SQUALENE

Resumen de: US20260226485A1

0000 Aspects of the present disclosure relate to modified yeast cells capable of producing increased quantities of squalene as compared to unmodified yeast cells and methods of using the same.

BIOSENSOR MATERIALS

Resumen de: US20260227395A1

0000 The disclosure provides a three-dimensional (3-D) macroporous melamine foam membrane comprising an amine reactive group. The membrane foam can be used to perform ELISA assays for the detection of an analyte. Diffusion of the analyte through the different membrane layers is fast and homogeneous in all directions and suitable for detection of large volume of samples in low concentration of targets and multiple targets simultaneously in one integrated system with minimal interference from targets.

METHOD FOR IDENTIFYING A MULTI-PARAMETER PHENOTYPE OF MICROBIOTA

Resumen de: US20260227396A1

0000 A method for identifying a multi-parameter phenotype of microbiota. The method includes (i) providing a sample including microbiota, (ii) labeling the microbiota with multiple labels, each of which binds a phenotypic parameter of the microbiota, (iii) detecting an intensity of the labelled phenotypic parameters of single cells of the microbiota by flow cytometry, and (iv) segmenting the single cells into bins based on the intensities of detected phenotypic parameters, wherein the distribution of single cells in bins represents a multi-parameter phenotype of said microbiota. Also described is a system for identifying a multi-parameter phenotype of intestinal microbiota, a kit for identifying a multi-parameter phenotype of intestinal microbiota and methods for diagnosing a medical condition associated with microbiota, for example an inflammatory condition, such as an inflammatory bowel disease, in a subject.

一种胶体金免疫层析病菌检测系统及其检测方法

Resumen de: CN122506156A

本发明公开了一种胶体金免疫层析病菌检测系统及其检测方法，涉及免疫测定技术领域，包括：获取待测液体样本；利用毛细作用使所述待测液体样本沿所述试纸条层析，与所述胶体金标记抗体结合形成免疫复合物；若样本中存在目标病菌，则所述免疫复合物在迁移至所述检测线时被所述捕获抗体捕获并显色；同时，未结合的胶体金标记抗体在所述质控线处被所述二抗捕获并显色；当层析前沿到达所述吸水垫时，通过智能读取终端采集所述试纸条的显色图像；将所述显色图像上传至云端分析平台，基于深度学习模型对检测线与质控线的显色强度进行定量分析，输出目标病菌的浓度值及阴阳性判定结果；本发明提升了胶体金免疫层析病菌检测的准确性。

基于噬菌体展示技术筛选的沙门菌模拟抗原表位肽及应用

Resumen de: CN122502436A

本发明适用于微生物技术领域，提供了一种基于噬菌体展示技术筛选的沙门菌模拟抗原表位肽及应用，其中，该沙门菌模拟抗原表位肽能够与抗沙门菌的多克隆抗体特异性结合，其包括如序列表SEQ ID NO:1‑4所示的一种或多种氨基酸序列。本发明筛选的沙门菌模拟抗原表位肽能特异性结合抗沙门菌的多克隆抗体，显著诱导机体体液免疫和细胞免疫应答，并对多种沙门菌血清型及产肠毒素大肠杆菌可以产生交叉保护，攻毒存活率高，安全性好。本发明为由革兰氏阴性病原体引起的疾病的预防和治疗提供了一种全新的有效疫苗。

一种具有免疫增强功能的蛋白、其编码基因及应用

Resumen de: CN122502457A

本发明公开了一种具有免疫增强功能的蛋白、其编码基因及应用。本申请所述具有免疫增强功能的蛋白的氨基酸序列如SEQ ID NO.1所示。该蛋白取自鼠伤寒沙门菌鞭毛蛋白FliC的C端部分序列，不仅保留了鞭毛蛋白固有的免疫增强活性，还显著降低了其免疫原性；将其应用于含铝佐剂的猪圆环病毒2型亚单位疫苗时，可有效增强该疫苗的免疫原性，诱导体液和细胞免疫应答，并显著促进抗原特异性的Th1和Th2型免疫应答，对完善现有含铝佐剂亚单位疫苗的配方、提升疫苗免疫效果具有重要意义。

Traditional Chinese medicine composition for treating salmonella pullorum as well as preparation method and application of traditional Chinese medicine composition

Nº publicación: CN122479009A 31/07/2026

Solicitante:

SHANDONG ANIMAL DISEASE PREVENTION AND CONTROL CENTER SHANDONG PROVINCIAL ANIMAL DISEASE PREVENTION
SHANDONG AGRICULTURAL UNIV
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Resumen de: CN122479009A

The invention discloses a traditional Chinese medicine composition for treating salmonella pullorum as well as a preparation method and application thereof, and belongs to the technical field of medical preparations. Screening is performed through network pharmacology, and the traditional Chinese medicine composition for treating salmonella pullorum is formed on the basis of the compatibility theory of monarch, minister, assistant and guide and the traditional Chinese veterinary pharmacy differentiation theory. The traditional Chinese medicine composition takes the root of Chinese pulsatilla and cortex phellodendri as monarch drugs, radix scutellariae and herba portulacae as ministerial drugs, agastache rugosus and rhizoma atractylodis macrocephalae as adjuvant drugs and liquorice as conductant drugs; the traditional Chinese medicine composition has the main effects of clearing away heat and toxic materials, eliminating dampness and stopping dysentery and the auxiliary effects of tonifying spleen and consolidating foundation, and has good treatment effect on salmonella pullorum