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84
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09/10/2025 [13:07:00]
Resultados 75 a 84 de 84
Resumen de: CN120285163A
The invention relates to the technical field of gene engineering, in particular to application of an RNF213 carboxyl terminal E3 structural domain or a coding gene thereof or a biological material containing the coding gene thereof in product preparation. The application of the RNF213 E3 structural domain in regulating the innate immune pathway is disclosed for the first time, and a powerful scientific basis and a tool are provided for prevention, control and treatment of bacteria such as salmonella and the like. The constructed vector containing the RNF213 E3 structural domain can significantly improve the expression level of immune factors, promote the activity of an NF-kappa B pathway and reduce the cell bacterium loading amount, provides a thought for scientific research and industrial application related to prevention and control of gram-negative bacteria such as salmonella, and lays a scientific foundation.
Resumen de: CN120290759A
The invention discloses a method and a kit for rapidly detecting campylobacter jejuni based on a specific molecular target glnp. The sequence of the molecular target is as shown in SEQ ID NO. 1. The PCR/qPCR detection kit for detecting campylobacter jejuni is established based on the target glnp, has extremely high specificity, only has a specific amplification signal for nucleic acid of campylobacter jejuni, and does not have any cross reaction for other common food-borne pathogenic bacteria such as escherichia coli, salmonella and staphylococcus aureus. Meanwhile, the sensitivity is high, and the nucleic acid detection target can detect the campylobacter jejuni genome DNA as low as 132fg/mu L. The method has the advantages of short detection time, high specificity, high detection sensitivity, simplicity in operation and the like.
Resumen de: CN120290758A
The invention discloses a campylobacter jejuni rapid detection method and a kit based on a specific molecular target rpoZ. The sequence of the molecular target is as shown in SEQ ID NO. 1. The PCR/qPCR detection kit for detecting campylobacter jejuni is established on the basis of the target rpoZ, has extremely high specificity, only has specific amplification signals for nucleic acid of campylobacter jejuni, and has no cross reaction for other common food-borne pathogenic bacteria such as escherichia coli, salmonella and staphylococcus aureus. Meanwhile, the sensitivity is high, and the nucleic acid detection target can detect the campylobacter jejuni genome DNA as low as 132fg/mu L. The method can effectively detect campylobacter jejuni in samples such as food, is high in specificity, good in accuracy and simple to operate, and can be applied to the fields of food safety and the like.
Resumen de: CN120290757A
The invention belongs to the technical field of biological detection, and discloses a multiple qPCR (quantitative polymerase chain reaction) kit for detecting food-borne pathogenic bacteria of aquatic products and application of the multiple qPCR kit. The kit comprises primers and a probe group for detecting vibrio parahaemolyticus, listeria monocytogenes and salmonella, and the sequences of the primers and the probe group are as shown in SEQ ID NO.1-9. According to the invention, probes with different fluorescence labels are utilized to realize simultaneous detection of three food-borne pathogenic bacteria of vibrio parahaemolyticus, listeria monocytogenes and salmonella in aquatic products. The method has the characteristics of short time consumption, good specificity, high sensitivity, high economic benefit and the like, has important significance on molecular diagnosis of the food-borne pathogenic bacteria in the aquatic products, and meets the detection requirements of simultaneously detecting the three food-borne pathogenic bacteria in the aquatic products.
Resumen de: CN120290363A
The invention discloses lactococcus formosanus with coaggregation capacity with helicobacter pylori and application of the lactococcus formosanus, and belongs to the technical field of microorganisms and the technical field of medicines. The lactococcus formosanus CCFM1461 screened by the invention has relatively strong gastric environment tolerance and acid production capacity, the copolymerization rate of the lactococcus formosanus CCFM1461 and helicobacter pylori within 120 minutes can reach 69.94%, the adhesion of the helicobacter pylori in AGS cells can be reduced, and the adhesion rate of the helicobacter pylori is reduced by 43.79%; the strain also has strong co-aggregation ability with other pathogenic bacteria (Escherichia coli, Staphylococcus aureus, Shigella, Salmonella typhimurium and Pseudomonas fluorescens), and has huge application prospects in the aspects of removal of helicobacter pylori, preparation of a helicobacter pylori adhesion scavenger and the like.
Resumen de: CN120285059A
The invention belongs to the technical field of veterinary drugs, and discloses a Chinese pulsatilla root alcohol extract and gentamicin composition and application thereof in inhibition of drug-resistant salmonella typhimurium. The pulsatilla alcohol extract composition provided by the invention comprises pulsatilla alcohol extract and gentamicin, and for salmonella typhimurium, the optimal ratio concentration of the pulsatilla alcohol extract to the gentamicin is 31.25 mg/mL: 2 mu g/ml; the Chinese pulsatilla root alcohol extract and the gentamicin have the synergistic bacteriostatic effect on salmonella typhimurium, the composition reduces the concentration of single bacteriostasis of the Chinese pulsatilla root alcohol extract and the gentamicin, the cost is saved, and the composition has wide application prospects and development value in the fields of food processing, medicine and breeding industry.
Resumen de: CN120290433A
本发明公开了一种基于鞭毛蛋白和LPS减毒的鼠伤寒沙门氏菌EN‑VNP菌株及其制备方法和应用，具体涉及生物技术领域。该菌株是通过在沙门氏菌VNP 20009菌株基础上，依次敲除或沉默鞭毛蛋白基因fliC、fljB、磷酸乙醇胺转移酶基因eptA、4‑氨基‑4‑脱氧‑L‑阿拉伯糖转移酶基因arnT、磷酸转移酶yeiU，并敲入或过表达磷酸转移酶基因lpxE形成的。本发明的鼠伤寒沙门氏菌EN‑VNP菌株具有较高的安全性，减毒后的菌株不含有鞭毛蛋白且LPS的脂质A分子仅保留一个磷酸基团，提高了菌株的安全性，使其更适合作为疫苗和药物递送载体。
Resumen de: CN120288855A
The invention discloses a preparation method of nano ozone water and application of the nano ozone water in killing food-borne pathogenic bacteria, and belongs to the technical field of bactericides. The nano ozone water is prepared by utilizing the ultra-fine bubble generator and the ozone generator, and the prepared nano ozone water is high in stability, not easy to decompose and high in practicability. The nano ozone water can achieve an effective killing effect on escherichia coli, salmonella, enterococcus, campylobacter and clinical multi-drug-resistant bacteria thereof, and a new resource is provided for preparing a safe and reliable antibacterial product.
Nº publicación: CN120285052A 11/07/2025
Solicitante:
JINLING INSTITUTE OF TECH
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Resumen de: CN120285052A
The invention discloses an application of herba schizonepetae essential oil in preparation of a salmonella pigeonae resisting medicine. The herba schizonepetae essential oil is extracted by adopting a steam distillation method. The herba schizonepetae essential oil provided by the invention shows an obvious inhibition effect on salmonella pigeons. The herba schizonepetae essential oil is natural in source and simple in preparation method, and has important application value in the aspect of preparing anti-salmonella pigeon.
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