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117
resultados
Última actualización
19/08/2026 [08:36:00]
Resultados 75 a 100 de 117
Resumen de: CN122150572A
The invention discloses an independent electrochemical immunosensing device for instantly detecting salmonella typhimurium. The invention firstly provides a bendable patch. The bendable patch comprises a microfluidic module and a sensing module, the sensing module comprises a substrate and an immune sensing layer arranged on the substrate, and a working electrode, a reference electrode and a counter electrode are arranged on the immune sensing layer; the working electrode is obtained by sequentially modifying a carbon electrode with 3D-PCNAs, EDC/NHS, a salmonella typhimurium antibody and bovine serum albumin; the microfluidic module includes a sealing layer, a spacer layer, and an automatic transport layer. The free-standing electrochemical immunosensing device provided by the invention comprises a bendable patch and an electronic module. The electrochemical immunosensing device is independent working equipment, has the characteristics of no need of a peristaltic pump and low cost, and is suitable for on-site instant detection of salmonella typhimurium.
Resumen de: CN122145623A
The invention relates to the technical field of molecular biology, in particular to an anti-salmonella enteritidis nano antibody Nb122 as well as application and a preparation method thereof, and relates to a nucleotide sequence and an amino acid sequence of the anti-salmonella enteritidis nano antibody Nb122. Meanwhile, the invention relates to application of the anti-salmonella enteritidis nano antibody Nb122 in preparation of a product for reducing adhesion and invasion of salmonella enteritidis to IPEC-J2 cells, and a preparation method of the anti-salmonella enteritidis nano antibody Nb122. According to the anti-salmonella enteritidis nano antibody provided by the technical scheme, the adhesion and invasion of salmonella enteritidis to IPEC-J2 cells can be reduced.
Resumen de: CN122140959A
The invention discloses an attenuated salmonella loaded adjuvant nano combined drug, which is characterized in that cyclodextrin is modified on the surface of bacteria by virtue of a condensation reaction of amino and carboxyl, and adamantane is modified on the surface of an adjuvant nano drug by virtue of a diselenide dynamic bond, so that the nano combined drug is prepared. Then adamantane and cyclodextrin are assembled through host-guest interaction of adamantane and cyclodextrin, so that the nano-drug is stably combined to the surface of attenuated salmonella. The diselenide dynamic bonds can be broken through ultrasonic response, so that the drug release effect is realized. The combined medicine has the tumor targeted enrichment capacity of bacteria and the characteristic that adjuvant nanoparticles are released under the action of ultrasonic waves, targeted delivery and deep penetration can be achieved in tumor tissue, and a remarkable tumor inhibition effect is achieved under stimulation of the ultrasonic waves. Experimental results show that the combined medicine shows a good treatment effect in the aspect of inhibiting tumor growth and has excellent biological safety at the same time.
Resumen de: CN122146539A
The invention relates to lactobacillus johnsonii for producing novel III-type bacteriocin, engineering bacteria and application of the lactobacillus johnsonii and the engineering bacteria, and belongs to the technical field of microorganisms. The lactobacillus johnsonii JLJ071 strain is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the lactobacillus johnsonii JLJ071 strain is CGMCC No.34541. The lactobacillus johnsonii JLJ071 strain can be used for preparing the lactobacillus johnsonii strain. The amino acid sequence of the bacteriocin is as shown in Seq ID No. 1. The expressed novel III-type bacteriocin has good bactericidal activity on listeria monocytogenes and salmonella pullorum, has the characteristics of good acid resistance, high temperature resistance, protease resistance and the like, and has a good application prospect in feed additives.
Resumen de: AU2026203814A1
The present disclosure relates generally to compositions, dosage forms, and methods for preventing and treating infections. The compositions include intact and substantially non- viable Gram-negative bacterial cells which have been treated to reduce lipopolysaccharide (LPS)-associated endotoxin activity, which surprisingly have increased activity to trigger immune cell production of cytokines. ay a y
Resumen de: CN122128407A
The invention discloses an LFS-SERS rapid quantitative detection method for salmonella, and belongs to the technical field of food safety, the method comprises the following steps: S1, cracking a sample to be detected to obtain a crude DNA solution; s2, carrying out salmonella specific LAMP (loop-mediated isothermal amplification) by taking the crude DNA solution as a template, so as to obtain a biotin labeled product; and S3, mixing dCas9 protein, sgRNA, the SERS-labeled gold nanoparticle-probe conjugate, the biotin labeled product and a loading buffer solution, incubating at room temperature for 1-5 minutes, carrying out a chromogenic reaction through a lateral flow test strip, and carrying out SERS detection on a T line in LFS by using a portable Raman spectrometer connected with an intelligent terminal to realize quantitative detection of salmonella in a to-be-detected sample. The method is strong in specificity, high in sensitivity and good in stability, the whole detection process can be completed within 40 minutes, the cost is low, and the operation is simple and convenient.
Resumen de: CN122124229A
The invention discloses a sodium caseinate-based Pickering emulsion as well as a preparation method and application thereof, and belongs to the technical field of vaccine adjuvants. The salmonella typhimurium flagellin and the sodium caseinate are used as raw materials to prepare the Pickering emulsion for the first time, and the prepared Pickering emulsion has the characteristics of small particle size, good biocompatibility and high stability, and can induce a body to generate higher immune response. The invention provides the application of the Pickering emulsion prepared by using salmonella typhimurium flagellin and sodium caseinate as raw materials as the vaccine adjuvant for the first time. The Pickering emulsion prepared by the preparation method disclosed by the invention is simple in steps, only needs two processes of mixing and homogenizing, and can effectively load a mode antigen OVA. The emulsion has good cell safety, and can promote endocytosis of macrophages to exogenous antigens. The emulsion can induce an organism to generate high-level antibody titer, and the antibody titer reaches more than 120,000 times.
Resumen de: CN122128190A
The invention relates to the technical field of microorganisms, in particular to streptococcus nonlyticus and application thereof in preparation of a salmonella infection resistant preparation. The invention provides a strain of streptococcus alactolyticus, which is preserved in the China Center for Type Culture Collection on March 30, 2026, and the preservation number of the streptococcus alactolyticus is CCTCC (China Center for Type Culture Collection) NO: M 2026565. After a host is subjected to intragastric administration of the streptococcus non-lactolyticus in advance, the resistance to salmonella typhimurium is obviously improved. After the host is infected with the salmonella typhimurium, the weight of the host is slowly reduced, the amount of the salmonella typhimurium in the liver and the spleen is inhibited, and the pathogenic bacterium load of the host is reduced.
Resumen de: CN122128143A
The invention belongs to the technical field of biology, and discloses bacillus amyloliquefaciens ZJ02 capable of inhibiting bacteria and reducing ammonia nitrogen, the preservation number is CCTCC NO: M20252399, the preservation date is October 30, 2025, the preservation unit is China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the bacillus amyloliquefaciens ZJ02 has an obvious antibacterial effect on escherichia coli and salmonella, and the bacillus amyloliquefaciens ZJ02 can be used for preparing a bacillus amyloliquefaciens strain. The size of an inhibition zone of salmonella (SA) in 24 hours is 18.11 mm; compared with other strains, the strain has a better ammonia nitrogen degradation effect under the same condition, the ammonia nitrogen degradation rate is 76.58%, and when the initial ammonia nitrogen concentration is 500 mg/L, the ammonia nitrogen degradation rate reaches up to 87.57%; besides, the bacillus licheniformis has excellent capability of reducing ammonia release of animal waste, and in a pig farm environment ammonia removal capability test, compared with a control group, the NH3 concentration is reduced by 60.27% on the sixth day and is obviously superior to that of bacillus subtilis and bacillus licheniformis; meanwhile, the invention also provides application of the bacillus amyloliquefaciens.
Resumen de: CN122123386A
The invention discloses application of a lactobacillus johnsonii cell-free supernatant in inhibition of a salmonella biofilm, and belongs to the technical field of microorganisms. The invention provides a new application of an acellular supernatant of lactobacillus johnsonii BNCC186110, and the acellular supernatant can inhibit the formation of biological membranes of salmonella typhimurium SL1344 and 14028, destroy the biological membranes of salmonella typhimurium SL1344 and 14028, and further reduce the colonization of salmonella typhimurium SL1344 and 14028.
Resumen de: MX2026005882A
Disclosed herein are methods and systems for rapid detection of microorganisms such as Listeria spp. in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Listeria-specific bacteriophage, allows detection of a specific microorganism, such as Listeria spp. and an indicator signal may be amplified to optimize assay sensitivity.
Resumen de: CN122104496A
The invention belongs to the technical field of microorganisms, and particularly relates to lactic acid bacteria and application thereof. The bacterial strain is Ligilbacillus salvarius SunLab-GHL02 (for short, L. salvarius GHL02), is separated from lungs of high-production-performance Aitujiaci broiler chickens, is preserved in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC NO: M 20251726. The invention further discloses a method for preparing the high-production-performance Aitujiaci broiler chicken with the high-production-performance Aitujiaci broiler chicken with the high-production-performance Aitujiaci broiler chicken with the high-production-performance Aitujiaci broiler chicken. Through morphology, physiology and biochemistry, 16S rRNA and whole genome identification, the classification status and functional characteristics of the gene are determined. The strain has good acid resistance, cholate resistance and acid production capacity, and can remarkably inhibit pathogenic bacteria such as escherichia coli, pathogenic escherichia coli, salmonella, staphylococcus aureus and pseudomonas aeruginosa. Animal experiments show that fowl growth performance and feed conversion rate can be improved, anti-stress capability can be enhanced and health can be promoted by atomizing the strain. The strain and the application thereof can be widely applied to development of feed additives, probiotic preparations and animal health-care pr
Resumen de: CN122104609A
The invention discloses a long-tailed bacteriophage and application thereof. The preservation name of the bacteriophage is WHSATYSB-2501, the preservation number of the bacteriophage is CCTCC No: M 2026038, and the bacteriophage is preserved in the China Center for Type Culture Collection on January 8, 2026. The bacteriophage can be used for preparing a bactericide or a disinfectant, and can also be used for inhibiting or cracking salmonella typhimurium. The long-tailed bacteriophage WHSATYSB-2501 obtained through separation can efficiently kill salmonella typhimurium in sewage, the titer can reach 6.8 * 10 < 11 > PFU/mL when the optimal infection complex number is 0.01, and the splitting and inhibiting effects on salmonella typhimurium are remarkably higher than those of existing bacteriophages. The application scene and the application range of the bacteriophage in salmonella prevention and control are remarkably widened, and an efficient and stable novel technical means is provided for biological prevention and control of salmonella.
Resumen de: CN122097342A
The invention discloses application of phellinus igniarius in preparation of a product for relieving salmonella-induced intestinal injury of chicks, and belongs to the technical field of veterinary drugs. In a salmonella enteritidis infected chick model, 20 mg/kg BW of phellinus igniarius is drenched to chicks. Experimental results show that the phellinus igniarius can significantly relieve the growth performance of chicks, improve the diarrhea condition and the fecal state of the chicks, and improve the intestinal structure and permeability through ileum M cells to relieve chick intestinal injuries caused by salmonella enteritidis. The method for resisting the enteritis type salmonella by the chicks, provided by the invention, is beneficial to replacing a traditional antibiotic treatment method and assists the development of poultry breeding.
Resumen de: CN122104492A
The invention discloses bacillus mucilaginosus for inhibiting systemic infectious, pneumonia and nerve type salmonella pullorum and application of the bacillus mucilaginosus. The bacillus mucilaginosus for inhibiting systemic infectious, pneumonia and nerve type salmonella pullorum is named as Anti-S. gallinarum, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M20251300.
Resumen de: CN122104522A
The invention discloses lactobacillus salivarius ZY-01 with salmonella resistance and anti-inflammatory repair functions, the strain is preserved in the China General Microbiological Culture Collection Center on March 9, 2026, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.37873. The strain can inhibit salmonella, escherichia coli and staphylococcus aureus, can reduce expression of proinflammatory factors such as IL-6, IFN-gamma and TNF-alpha by inhibiting a JAK-STAT signal channel, can promote expression of an IL-10 anti-inflammatory factor and mucous membrane repair factors such as EGF, EGFR and TGF-beta1, and can achieve intestinal tract anti-inflammation and mucous membrane repair. The lactobacillus salivarius ZY-01 and the bacterial liquid, the bacterial powder and the fermentation product thereof can be used as core raw materials of animal feed additives, microecologics and veterinary drugs, are used for preventing and controlling salmonella infection in animal breeding and improving animal intestinal health, and have wide application prospects.
Resumen de: US20260144857A1
Technologies for the prevention and/or treatment of Shigella infections.
Resumen de: US20260146994A1
A system and method for detecting, enumerating, or extracting microorganisms in a sample is disclosed. Target microorganisms, such as Salmonella bacteria, may be of interest. Magnetic beads may be bound to the target microorganisms. After which, the bead-bound cells may be isolated. For example, a magnetic field may be applied in order to separate the target cells (with the magnetic beads attached thereto) and move then to a predetermined section of the well. Agar, or other immobilizing agent, may be added to the wells in order to immobilize the target cells. After which, the target cells are incubated and periodically analyzed to determine whether the target cells are growing, thereby indicating that the microorganisms are contained within the well.
Resumen de: WO2026108702A1
Disclosed in the present invention are a nanobody for detecting Escherichia coli O157:H7 and a preparation method therefor. The present invention aims to provide a nanobody for detecting Escherichia coli O157:H7 with high detection accuracy and a simple acquisition method. The amino acid sequence of the nanobody is set forth in SEQ ID NOs. 1-3. The present invention belongs to the technical field of biopharmaceuticals.
Resumen de: WO2025021712A1
The present invention relates to immunogenic compositions comprising antigens from Salmonella enterica serovar Typhimurium (S. Typhimurium), Salmonella enterica serovar Enteritidis (S. Enteritidis), and Salmonella enterica serovar Typhi (S. Typhi). The present invention further relates to methods and uses of compositions comprising GMMA for boosting an immune response to an S. Typhi antigen, vaccines comprising the immunogenic compositions and methods and uses of the immunogenic compositions.
Resumen de: WO2025021695A1
The present invention relates to immunogenic compositions comprising outer membrane vesicles (OMVs) adsorbed to an aluminium hydroxide adjuvant, whereby the aluminium hydroxide adjuvant is quenched. The present invention also relates to the use of the immunogenic compositions for providing protection against diseases caused by Gram-negative bacteria. The present invention also relates to a method for reducing particle aggregation and/or average particle diameter in compositions comprising OMVs and an aluminium hydroxide adjuvant.
Resumen de: CN122081526A
The invention belongs to the technical field of molecular biological detection, and particularly relates to a primer and probe combination for detecting avian salmonella based on an RAA-LFD method and application of the primer and probe combination. The invention particularly relates to a primer and probe combination for detecting three avian serotype salmonella such as salmonella pullorum, salmonella enteritidis and salmonella typhimurium based on an RAA-LFD method and application of the primer and probe combination. Different types of probes and primers are respectively designed aiming at genes of serotype salmonella SP, SE and STM, rapid, sensitive and specific detection of salmonella and three serotype avian salmonella such as SP, SE and STM can be realized by adopting the primer and probe combination, the detection period of avian salmonellosis is greatly shortened, the detection time is shortened, and the detection efficiency is improved. The detection efficiency and accuracy of the avian salmonellosis are improved.
Resumen de: CN122081240A
0001 本发明公开了一种沙门菌H:i抗原表位特异性单克隆抗体及其应用，其由保藏编号为CCTCC NO:C202645的杂交瘤细胞分泌得到，该抗体具有效价高、特异性强的优势。本发明以此抗体构建的阻断ELISA检测试剂盒及其检测方法可特异性检测感染动物血清中含H：i抗原表位沙门菌的抗体，且与不含H:i抗原表位沙门菌及非沙门菌的抗体均不反应，敏感性、特异性和稳定性良好，为鼠伤寒和肯塔基等含H:i抗原表位沙门菌感染的直接血清学检测提供了新方法。
Resumen de: CN122087393A
The embodiment of the invention provides a chicken salmonella intestinal lesion spectral characteristic pattern recognition method and system, and relates to the technical field of chicken salmonella intestinal lesion spectral characteristic pattern recognition, and the method comprises the steps: obtaining a chicken intestinal tissue original spectrum; determining a distortion mode of the surface of the spectral probe; correcting the original spectrum according to the distortion mode to obtain a corrected spectrum; matching the corrected spectrum with a standard lesion spectrum, and calculating a matching degree between the corrected spectrum and the standard lesion spectrum to obtain a spectrum matching degree; and according to the spectrum matching degree, judging whether chicken intestinal tissues have chicken salmonella lesion or not. According to the invention, the detection accuracy and reliability of the spectrum identification system in a complex environment of a large-scale chicken farm can be improved.
Nº publicación: CN122081173A 26/05/2026
Solicitante:
GUANGXI ACAD OF AGRICULTURAL SCIENCES
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Resumen de: CN122081173A
The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LT303 and application thereof.The lactobacillus plantarum strain LT303 is separated from an acid material, and a fermentation product obtained after fermentation of the strain and leaf mustard has the antibacterial effect on salmonella enteritidis, staphylococcus aureus and/or listeria monocytogenes; the content of sweet amino acids, namely threonine, alanine, serine, glycine and proline, of the pickled Chinese cabbages prepared by fermentation of the strain LT303 is remarkably high, and the fermented pickled Chinese cabbages are the most sweet and fresh in taste and the most abundant in sweet amino acid content; besides, the texture of the pickled Chinese cabbages prepared by adding the mango juice, the pitaya juice and the siraitia grosvenorii juice is enhanced, and the content of sweet amino acids is more remarkably increased, so that the LT303 strain can be used for preparing the pickled Chinese cabbages which are more sweet in taste and more unique in flavor, fermentation liquor has the value of researching and developing antibacterial peptides, and new raw materials and thoughts are provided for increasing the additional value of products in the later period.
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