Absstract of: CN121022664A
The invention provides a cyanobacteria strain for producing phenylalanine and application of the cyanobacteria strain, and belongs to the technical field of functional cyanobacteria. The invention provides a cyanobacteria strain for producing phenylalanine, which is a synechocystis sp. Strain UV120, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.46456. The invention also provides a preparation method of the cyanobacteria strain for producing phenylalanine. According to the invention, two mutagenized blue algae strains are obtained by adopting ultraviolet mutagenesis, and the strain UV120 not only has a good growth characteristic under the condition of high-concentration L-phenylalanine or analogues thereof, but also has the characteristic of high yield of L-phenylalanine, so that a new means is provided for large-scale synthesis of phenylalanine.
Absstract of: CN121038801A
Compositions comprising microalgal extracellular vesicles (MEVs) formulated for intranasal delivery are provided whereby, after intranasal administration, the MEVs are transported by a specific route, via olfactory nerves to specific regions in the brain after intranasal administration, and through lateral olfactory beams (LOTs) to interconnected brain regions. The MEV is transported via neuronal axons. The MEV has the ability to traverse synapses including: (i) a synapse between an olfactory sensory neuron (OSN) and a monk cap/clustered neuron; (ii) synapses between monk cap/cluster neurons and local neurons in a plurality of brain regions colonized by a lateral olfactory beam (LOT); and (iii) synapses between neurons in the brain region colonized by LOT and neurons from the frontal cortex, hippocampus, thalamus and hypothalamus. The compositions contain extracellular vesicles (MEVs) from microalgae loaded with biologically active cargo for use in treating, detecting, diagnosing or monitoring a disease, disorder or condition of the brain or involving the brain, in particular to provide neuronal delivery of cargo. The compositions and methods have various applications as therapeutic and diagnostic agents for treating, diagnosing, and monitoring the brain or a disease, disorder, or condition involving the brain. The compositions are useful in methods and uses for the treatment of cancers involving the brain, and are useful, for example, for the delivery of therapeutic agents f
Absstract of: CN121021291A
The invention provides a method for comprehensively extracting eicosanoic acid containing EPA and ARA from phaeodactylum tricornutum and application of eicosanoic acid, a complete process chain of algae culture, algae mud acquisition, rapid ASE extraction of microalgae oil, SPE enrichment and semi-preparative HPLC purification is established, the anti-inflammatory medium is definite in composition proportion, and the structure is identified through high performance liquid chromatography; existing literatures and patents do not disclose a combination of the algal strain capable of enriching the multiple anti-inflammatory media at the same time. The raw material of the phaeodactylum tricornutum is from marine diatom which can be cultured on a large scale, the risk of pesticide residue of terrestrial plants is avoided, an organic chlorine solvent is avoided in the production process, and the requirement of FDA/EFSA on natural marine source functional ingredients is met; the compound can be used for anti-inflammatory prescription medicines, postoperative inflammation relieving foods and relieving and repairing cosmetics at the same time, and the market application range is wide.
Absstract of: CN121030277A
The invention provides an intelligent treatment method for mariculture tail water, and belongs to the technical field of machine learning. By constructing a multi-mode tail water environment-evolution data set, comprehensive perception of a tail water visual image sequence, physicochemical index time sequence data and operation control parameters is realized, and dynamic evolution prediction of a tail water system is carried out based on a virtual environment generation model. Through the steady state identification and mode mining module, the stable operation cluster, the critical boundary and the abrupt change mode of the tail water system can be quantified, control-abrupt change response knowledge is extracted, and a structured basis is provided for optimization decision making. On the basis, a multi-target control optimization model is constructed, efficient regulation and control over the microalgae growth rate, the suspended particle sedimentation rate and the nutritive salt absorption efficiency are achieved, meanwhile, the potential mutation risk is avoided, and steady-state operation of the system is guaranteed. According to the method, intelligentization, precision and multi-objective optimization of the tail water treatment process can be achieved, and efficient and reliable technical support is provided for mariculture tail water ecological management.
Absstract of: CN121021241A
The invention discloses a production method of a microalgae water-soluble fertilizer based on swine urine. The production method comprises the following steps: S1, collecting swine urine; s2, primary treatment of swine urine; s3, carrying out mold removal and detoxification on the swine urine; s4, culturing the microalgae; s5, strengthening the microalgae; s6, collecting microalgae; s7, preparation of the water-soluble fertilizer: in the step S1, firstly filtering the pig urine by using a 100-150-mesh filter screen to remove feed residues or excrement particles, and in the step S2, firstly diluting the pig urine until the nitrogen content in the pig urine is 100-300mg/L, then carrying out catalytic ozonation on the pig urine, adding ferric oxide and activated carbon into the inner side of the pig urine according to a ratio of 2: 1, and uniformly stirring to obtain the water-soluble fertilizer. The ratio of the ferric oxide and the activated carbon to the swine urine is 1: 10, the microalgae water-soluble fertilizer with low heavy metal pollution is prepared by sufficiently treating the swine urine, then adjusting the microalgae and finally combining the swine urine with the microalgae, and the microalgae water-soluble fertilizer can be used in a farmland in a large area and is high in use efficiency.
Absstract of: CN121020826A
The invention discloses a method for constructing an Algammox system, and relates to a method for constructing a system. The invention aims to solve the problems that the existing anaerobic ammonium oxidation bacteria (AnAOB) are easily damaged by ROS (reactive oxygen species) induced by light irradiation and are greatly influenced by DO (dissolved oxygen). According to the invention, microalgae is innovatively introduced into a PNA technology, and an Algammox system is constructed. Dissolved oxygen is supplied in situ by utilizing microalgae through photosynthesis, so that the dependence on mechanical aeration is reduced; meanwhile, a biofilm carrier strategy is adopted, so that the inhibition of illumination on AnAOB is effectively shielded, and the biomass retention capacity is enhanced. The result shows that the total nitrogen removal rate of the developed Algammox system is as high as 97%. The invention belongs to the technical field of biological environmental protection and biological energy.
Absstract of: CN121022548A
The invention discloses an intelligent sampling and on-line detection device and method for microalgae culture, and relates to the technical field of biological culture on-line detection. The microalgae stock solution detection unit comprises a stock solution detection pool, two sides of the stock solution detection pool are respectively provided with a divergent light source and a spectrum detection sensor, and the microalgae stock solution detection unit is used for detecting microalgae pigment and cell concentration in the stock solution through spectrum inversion; the microalgae diluent detection unit comprises a diluent detection pool, and a plurality of diluent detection sensors are arranged in the diluent detection pool and used for detecting the pH value, the conductivity and the inorganic salt ion concentration in the diluent; and the automatic sampling and diluting unit comprises a PLC (Programmable Logic Controller) as well as a first peristaltic pump, a second peristaltic pump, a first electromagnetic valve, a second electromagnetic valve and a plurality of liquid level meters which are electrically connected with the PLC, and is used for controlling linkage operation of each component and completing stock solution sampling, solution changing, stock solution detection pool cleaning, stock solution diluting, solution discharging and diluent detection pool cleaning. According to the invention, effective detection of multiple parameters of the microalgae culture syst
Absstract of: CN121020835A
The invention discloses a method for improving livestock and poultry breeding wastewater treatment by a phycomycete system through starch wastewater and application, and belongs to the technical field of environmental engineering and biological recycling. The method comprises the following steps: (1) pretreating livestock and poultry breeding wastewater and starch wastewater to remove impurities; livestock and poultry breeding wastewater and starch wastewater are mixed according to a certain volume ratio to prepare mixed wastewater; (2) mixing chlorella sorojin and aspergillus oryzae in a logarithmic phase according to a certain quantity ratio, and inoculating to construct a microalgae-fungus co-culture system; and (3) putting the microalgae-fungus co-culture system into the mixed wastewater, treating under an illumination oscillation condition, and purifying the mixed wastewater. According to the method, the starch wastewater is adopted as a novel carbon source for replacing a traditional commercial carbon source, and a biological treatment mode of microalgae-fungus co-culture is combined, so that efficient purification of the livestock and poultry breeding wastewater is realized, meanwhile, biomass accumulation is promoted, resource utilization of wastes is realized, and dual targets of wastewater treatment and resource recovery are achieved.
Absstract of: WO2025241022A1
The present invention provides a process for obtaining an extract with a high lutein content from microalgae, comprising a microalgae cultivation process with high biomass and lutein production, as well as a process for extracting lutein and chlorophylls using green solvents. The microalgae belong to the genus Chlorella from Brazilian biodiversity, which produces metabolites comprising lutein and chlorophylls. The cultivation process for the microalgae of the genus Chlorella was optimized, and different carbon and nitrogen sources used in the culture medium were evaluated with respect to lutein production. The lutein extraction process employs solvents such as ethanol and glycerin, which are safe for cosmetic and nutraceutical applications. The obtained extract has a high lutein content and also contains chlorophylls and neoxanthin. Lutein assists in protecting the skin against photoinduced damage, and the extract with a high lutein content is suitable for use in cosmetic compositions, pharmaceutical compositions, and the food industry.
Absstract of: WO2025243087A2
The present disclosure relates to compositions, nutraceutical compositions, and dietary supplements that improve gut health, enhance energy, improve focus and energy, reduce cravings, improve stress management, improve overall health, promote healthy aging, replenish nutrients, support immune health, promote healthy skin, promote hair and nail growth, and enhance recovery after physical activity. The compositions comprise one or more of each of seaweeds, enzymes, and protein; two or more of each of adaptogens, botanicals, extracts, fibers, microalgae, micronutrients, minerals, vitamins, and whole fruit or vegetable powders; and three or more probiotics.
Absstract of: WO2025243318A1
The present disclosure provides a microbiological process for the production of hydroquinone from benzoquinone in a microalgal system. The method is particularly characterized by the extraction of the hydroquinone product at a specific points in the process followed by re- introduction of the benzoquinone substrate into the microalgal system. The said method of the 0 present disclosure allows sustained production of hydroquinone.
Absstract of: CN121005766A
The invention discloses an application of a transcription factor ChZF7 of a basophilic microalgae Chlorella sp. BLD in regulation and control of salt tolerance of microalgae. According to the invention, the coding gene of the transcription factor ChZF7 is transferred into chlamydomonas reinhardtii by using a transgenic technology after intron insertion and codon optimization, so that transgenic chlamydomonas reinhardtii is obtained. The salt tolerance of the chlamydomonas reinhardtii with the over-expressed ChZF7 gene is remarkably improved, the accumulation of neutral fat and active oxygen under the stress of 225mM salt is less than that of the chlamydomonas reinhardtii in a control group, the content of penetrating substance proline is increased, in addition, the change of cell components in the chlamydomonas reinhardtii under the stress of salt can be weakened by the over-expressed ChZF7 gene, and the salt tolerance of the chlamydomonas reinhardtii is improved. The transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 can regulate and control the salt tolerance of the chlamydomonas reinhardtii, a foundation is laid for genetic improvement and directed molecular breeding of the microalgae, the transcription factor ChZF7 plays an important role in improvement of the salt tolerance of the microalgae, reutilization of salinized water resources is facilitated, and the transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 have
Absstract of: CN121006354A
The invention relates to a microalgae carbon sequestration technology, and aims to provide a method for promoting microalgae carbon sequestration by using a transparent film carrying a nitrogen-doped carbon dot coating. According to the method, a transparent film is arranged on the outer side of the photobioreactor in a surrounding manner, so that external light rays penetrate through the transparent film and then enter the photobioreactor; the nitrogen-doped carbon dot coating is carried on the surface of the transparent film, ultraviolet light of 280-390 nm in external light is converted into blue light of 400-500 nm through the coating, and growth and carbon sequestration are promoted while damage to algae cells is reduced. Damage to algae cells can be greatly reduced by converting ultraviolet rays, and meanwhile, the ultraviolet rays are converted into blue light to further promote microalgae growth and carbon sequestration. The light source improvement effect of the photobioreactor can be comprehensively improved, the entering blue light source is enhanced and more uniform, and spectral distribution is optimized; the method can be used for manufacturing the composite film in an industrial production scale and transforming the photobioreactor in batches, and is low in cost, simple to operate and suitable for popularization and application.
Absstract of: CN121003297A
The invention discloses a preparation method of a spirulina-fish oil nano-emulsion and application of the spirulina-fish oil nano-emulsion in fish oil microcapsules, and belongs to the technical field of microalgae application, and the preparation method specifically comprises the following steps: adding spirulina powder into pure water, and magnetically stirring uniformly to obtain a spirulina suspension; dropwise adding fish oil into the spirulina turbid liquid, and pre-emulsifying by using a high-speed dispersion homogenizer during the period to prepare a spirulina fish oil crude emulsion; treating the crude spirulina fish oil emulsion by using a high-pressure micro-jet homogenizer to obtain the spirulina-fish oil nano-emulsion. And carrying out spray drying on the spirulina-fish oil nano-emulsion by using a spray drying technology to obtain the spirulina-fish oil microcapsule. The spirulina fish oil microcapsule prepared by the invention has relatively good oxidation stability and thermal stability, can well cover the fishy smell of the fish oil, is easy to transport and store, and is more easily accepted by consumers.
Absstract of: CN121006355A
The invention relates to a microalgae photosynthetic carbon sequestration technology, and aims to provide a method for promoting microalgae carbon sequestration by a polyethylene glycol passivated nitrogen-doped carbon dot conversion spectrum. The method comprises the following steps: passivating the surfaces of nitrogen-doped carbon dots by using polyethylene glycol, and then directly adding the passivated nitrogen-doped carbon dots into a microalgae suspension, ultraviolet light with the wavelength of 280-390 nm in external light is converted into blue light with the wavelength of 400-500 nm by passivating the nitrogen-doped carbon dots so as to be directly utilized by microalgae, so that growth and carbon sequestration are promoted while the stress resistance of algae cells is improved. According to the method, damage to algae cells can be greatly reduced by converting an ultraviolet spectrum, and meanwhile, microalgae growth and carbon sequestration can be further promoted by converting ultraviolet light into blue light; the spectrum conversion effect is enhanced, the overall light energy utilization efficiency of microalgae carbon sequestration is improved, light inhibition is relieved, and the stress resistance of microalgae cells is improved, so that microalgae growth carbon sequestration is promoted, and the development of the microalgae carbon sequestration industry is promoted.
Absstract of: CN120988812A
The invention relates to the technical field of microalgae culture, in particular to an air supply and temperature regulation system of a floating microalgae photobioreactor and application of the air supply and temperature regulation system, the air supply and temperature regulation system comprises a floating platform, a plurality of incubators are arranged in the floating platform, an air supply system is arranged among the incubators, and a driving mechanism is further arranged above the floating platform. According to the air supply and temperature regulation system of the floating microalgae photobioreactor and the application thereof, the air bag is inflated and deflated to drive overall floating and sinking, stability and reliability of floating and sinking actions are ensured by utilizing the characteristic of high corrosion resistance of the air bag, and an inserting rod in a limiting pipe drives a round rod to rotate through matching of a convex block and a spiral groove in the fixed-distance rising process; therefore, the three groups of limiting blocks are controlled to pop out in sequence, three different mechanical limiting points are formed, and the multi-stage accurate control of the immersion depth of the incubator is realized.
Absstract of: CN120997315A
The invention discloses a microalgae density measurement method based on image color correction, which comprises the following steps: providing stable illumination by using an LED panel light source under a pure white background, collecting images of microalgae samples with different densities, and constructing a color difference-density model of microalgae images; extracting the RGB average value of the whole image and calculating the weighted combination chromatic aberration of the RGB average value and the ideal white color; the method comprises the following steps: selecting a plurality of pure color backgrounds with different colors and different materials, collecting microalgae sample images, and setting a zero-density microalgae sample as a reference standard for correcting system errors; the image is processed through a color correction algorithm, chromatic aberration offset caused by the background is compensated, and the microalgae image approximates to the expression under the pure white background in a color space; according to the method, the influence of background color on the microalgae image can be corrected, the detection process is simplified, the accuracy and reliability of microalgae density measurement are improved, and the method is suitable for microalgae density monitoring under various environmental conditions.
Absstract of: CN120982448A
The invention relates to the technical field of monomeric oyster fry cultivation, and discloses a monomeric oyster fry attachment material and a preparation method thereof. The monomer young oyster attaching material comprises a fiber fabric base body and attaching layers loaded on the two surfaces of the fiber fabric base body. The adhesion layer is prepared from a film forming material; the film-forming material is prepared from the following raw materials: oyster shell powder, crushed oyster shells, carbon powder, living microalgae, an adhesive and water. According to the monomer young oyster attaching material provided by the invention, the fiber fabric matrix with flexibility and biodegradability is used as a framework, components in the attaching layers on the two sides can be biodegraded or physically broken, the structure is kept stable in an expected service cycle, and a stable structure is provided for attachment of young oysters; and the young oysters are not easy to break in the stripping process, the adhesion layer is not left after stripping, and the pollution to the breeding environment is avoided.
Absstract of: CN120988813A
The invention relates to the technical field of microalgae culture, in particular to a floating microalgae culture photobioreactor system and application thereof.The floating microalgae culture photobioreactor system comprises a grid frame fixedly arranged underwater, and a plurality of liftable culture containers are arranged in grid units of the grid frame; displacement sensors and temperature sensors are installed on the culture containers, lifting mechanisms are arranged at grid units of the grid frame, and each lifting mechanism comprises a servo electric cylinder and a pressing rod connected with the plurality of culture containers through elastic supporting pieces; the central controller receives data of the sensor and controls the servo electric cylinder to dynamically adjust the water immersion depth of the plurality of culture containers according to different time periods of a preset day. The lifting mechanism driven by the servo electric cylinder, the linkage elastic supporting piece and the culture container are arranged in the grid frame, and the height of the container is actively adjusted according to a preset time-depth curve in combination with the central controller, so that the temperature of algae liquid is stably maintained at normal temperature, and the problem of high-temperature death caused by strong light in summer is solved.
Absstract of: CN120997450A
The invention discloses an enteromorpha-microalgae competitive growth process simulation method, and relates to the technical field of marine ecology and environmental science. The method comprises the following steps: taking remote sensing image monitoring data of a research area, constructing an enteromorpha green tide coverage area three-dimensional space-time distribution matrix, and determining the distribution range of the matrix; dividing and numbering according to the size of a preset grid unit to obtain an enteromorpha block and a spatial position, and performing dimension reduction processing; the method comprises the following steps: dividing a research area into three subareas, and respectively constructing nutritive salt-enteromorpha-microalgae-debris ecological dynamics box-type models; determining a cross-regional shunting coefficient according to the drifting speed of the enteromorpha between the partitions, and connecting the three box-type models in series; and reconstructing and simulating a time-varying simulation value of the enteromorpha coverage area by using the serially connected models, determining an environmental element modulation effect value, and further determining the key control degree of each environmental element on the competitive growth process of the enteromorpha.
Absstract of: CN120989069A
The invention provides a preparation method of microalgae gel balls for water treatment, and relates to the field of water treatment, and the preparation method comprises the following steps: step S1, preparing a composite suspension, step S2, carrying out primary crosslinking to form balls, step S3, carrying out secondary crosslinking, step S4, carrying out in-situ enhancement, and step S5, coating. According to the method, microalgae is subjected to compound modification to obtain gel beads, and the finished product gel beads are obtained through coating, so that the swelling resistance and mechanical strength of the gel beads are improved, and the situation that a compact gel network and a multi-layer coating structure hinder nutrient substances, carbon dioxide and the like in a water body from diffusing to microalgae cells is avoided; the photosynthesis efficiency and pollution degradation capability of the microalgae are ensured, and the water treatment efficiency of the microalgae is improved.
Absstract of: CN120987475A
The invention relates to a microalgae bioreactor and a sewage treatment method. The microalgae bioreactor comprises a shell, a filter bed, an LED light source and a spraying device, a water outlet and a reflux inlet are formed in the lower part of the shell; the filter bed is made of a porous material and is arranged in the shell; the number of the LED light sources is at least one, and the LED light sources are arranged outside and/or inside the shell; the spraying device is arranged above the filter bed; a water inlet connected with a sewage inlet pipeline is formed in the spraying device; the water inlet is also connected with the reflux inlet through a reflux pipeline. According to the microalgae bioreactor disclosed by the invention, nitrogen and phosphorus substances in sewage are effectively removed by utilizing a microalgae biological membrane, and the effect of recycling nitrogen and phosphorus nutrient elements is realized.
Absstract of: CN120988056A
The invention discloses a preparation method of a chlamydomonas reinhardtii polypeptide composition, and belongs to the technical field of natural active ingredients. The preparation method comprises the following steps: respectively carrying out nitrogen deficiency stress culture on chlamydomonas reinhardtii cc620 and cc621 strains, mixing, standing for mating, and centrifugally collecting mating supernate; adding the mating supernatant into the chlamydomonas reinhardtii fresh cell suspension, after cell wall hydrolysis removal, centrifugally collecting the supernatant to obtain cell wall hydrolysate, and resuscitating and culturing the protoplast precipitate to be circularly used for cell wall hydrolysis removal; the cell wall hydrolysate is subjected to nanofiltration, concentration and impurity removal are achieved, and the chlamydomonas reinhardtii polypeptide composition can be obtained. The chlamydomonas reinhardtii polypeptide composition prepared by the invention is a product rich in hydroxyproline and arabinose modified oligopeptide, and shows relatively high bioavailability in a rat oral pharmacokinetic test. The potential application of the microalgae-derived collagen oligopeptide analogue provided by the invention relates to the fields of foods, health foods, cosmetics, medicines and the like.
Absstract of: CN120988846A
The invention discloses a culture method and application of beta-1, 3-glucan-producing microalgae, and realizes high-efficiency synergy from microalgae culture to separation. According to the enrichment culture medium, by optimizing the ratio of carbon and nitrogen sources and nutritional ingredients, efficient accumulation of beta-1, 3-glucan can be remarkably improved, meanwhile, the microalgae biomass is promoted, and the problem of low product content in conventional culture is solved. The matched microalgae culture method breaks through the limitation that traditional plate streak bacterial colonies are mixed, water drop separation consumes time and is prone to pollution, the high-purity single strain is accurately extracted from a complex water sample, the operation standardization degree is high, and the manual randomness influence is reduced. Through the synergistic application of the two, the microalgae with high biomass and high glucan content can be efficiently obtained, the separation purity can be guaranteed, high-quality resources are provided for scientific research and industrial production, and the application of the microalgae in the fields of food, medicine and the like is promoted.
Nº publicación: CN120988904A 21/11/2025
Applicant:
CENTRAL CHINA NORMAL UNIV
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Absstract of: CN120988904A
The invention discloses a compound microbial agent and application thereof in prevention and control of cyanobacterial bloom, two algicidal bacteria are obtained through screening, the preservation number is CCTCC NO: M 2024698, and the preservation number is CCTCC NO: M 2024699, and the compound microbial agent is proved to have obvious inhibition effects on a plurality of cyanobacteria bacteria such as microcystis aeruginosa, lacustrine triphora, anabaena and cylindrocystis, and can be used for preventing and controlling cyanobacteria bloom. And the composite bacterial agent combined by the two algicidal bacteria has a remarkable algicidal effect on algal blooms in natural water areas, can be applied to the field of biological prevention and control of freshwater cyanobacterial blooms, and provides an ideal choice for treatment of cyanobacterial blooms in water bodies.