Absstract of: CN119506138A
The invention relates to a method for improving soil fertility and enzyme activity of a rice field by using a nitrogen-fixing cyanobacteria biological membrane, which comprises the following steps: step 1, expanding propagation of nitrogen-fixing cyanobacteria: selecting anabaena globifera as a rice field application algae species, and performing expanding culture on the algae species to obtain a nitrogen-fixing cyanobacteria secondary algae species; step 2, applying nitrogen-fixing cyanobacteria: applying the nitrogen-fixing cyanobacteria in a rice base fertilizer period and a tillering period, and replacing part of chemical fertilizer with the nitrogen-fixing cyanobacteria to reduce the application amount of the chemical fertilizer; secondly, the nitrogen-fixing cyanobacteria returns to the rice field through biological nitrogen fixation and rotting decomposition of dead algae, the content of nitrogen and carbon in the soil is increased, the soil fertility is improved, and the nitrogen-fixing cyanobacteria can provide nutrient substances for microorganisms in the soil, improve the enzyme activity of the soil and improve the quality of the soil when applied to the rice field.
Absstract of: CN119522288A
The present disclosure provides a method for determining exfoliation of an outer membrane of cyanobacteria capable of easily determining whether or not the outer membrane has been exfoliated from a cell wall. A method for determining exfoliation of an outer membrane of cyanobacteria according to the present invention comprises: a measurement step (S01) for measuring the concentration of at least one amino acid selected from the group consisting of isoleucine, leucine, tyrosine and phenylalanine in a culture supernatant of cyanobacteria; and a determination step (S02) for determining whether or not the outer membrane of the cyanobacteria has been peeled off from the cell wall on the basis of the concentration of the at least one type of amino acid measured in the measurement step.
Absstract of: CN119506048A
The invention relates to the field of microalgae culture, in particular to a closed marine microalgae culture system which comprises a greenhouse, a water pool is arranged in the greenhouse, a buoyancy device and a wave making device float in the water pool, a photosynthesis device is arranged on the buoyancy device and is in an upward convex shape, and the photosynthesis device comprises a transparent outer shell and a transparent inner shell. A photosynthesis cavity is formed between the transparent outer shell and the transparent inner shell, an exhaust valve is arranged at the top of the transparent outer shell and communicated with the photosynthesis cavity, the bottoms of the transparent outer shell and the transparent inner shell are both connected with the annular bottom shell in a sealed mode, and a feeding port and a discharging port are formed in the annular bottom shell. A gas uniformizing component is arranged at the bottom of the photosynthesis cavity. The greenhouse provided by the invention can provide a good environment for marine microalgae culture, and is beneficial to the growth of microalgae; meanwhile, the light receiving area of the photoreactor is large, and algae are not easily adsorbed on the container wall; the photoreactor floats in the water tank, so that stirring of a culture solution in the photoreactor is facilitated.
Absstract of: CN119506068A
The invention discloses a control method for bacterial pollution in microalgae culture and a culture device thereof, and relates to the field of microalgae culture, and the control method comprises the following steps: step 1, carbon dioxide supply: when microalgae are cultured in a transparent incubator, when a carbon dioxide sensor detects that carbon dioxide in the incubator is smaller than a preset interval, the carbon dioxide sensor detects that carbon dioxide in the incubator is smaller than the preset interval; the valve is opened, so that the carbon dioxide enters the air supply pipeline through the communicating pipe, the circular reducer pipe and the connecting pipe, and then is sprayed into the nutrient solution from the spray head; 2, carbon dioxide is dissolved, when the carbon dioxide passes through a round reducer pipe, a driving assembly in a treatment mechanism is driven, namely, an impeller rotates, the impeller transmits rotating force to a third straight gear, the rotating third straight gear drives a gear ring in a stirring assembly to rotate, and the carbon dioxide is stirred; a gear ring drives a stirring rod to stir a nutrient solution by controlling a horizontal plate to cooperate with sprayed bubbles, so that the dissolution of carbon dioxide is accelerated, the growth conditions of microalgae are optimized, and the growth of bacteria is inhibited; and step 3, cleaning the inner wall of the box: when a horizontal plate moves, pushing a pushing block
Absstract of: CN222524495U
The utility model discloses a circular microalgae reactor, which belongs to the technical field of microalgae biology and comprises a transparent cylindrical reaction tank, a feed port and a discharge port are respectively arranged at the upper end and the lower end of the cylindrical reaction tank, a stirring mechanism is arranged in the cylindrical reaction tank, and an annular illumination mechanism is arranged on the cylindrical reaction tank. The annular illumination mechanism comprises an annular flexible light guide plate and an LED lamp arranged on the annular flexible light guide plate. Microalgae culture solution and microalgae are added into the cylindrical reaction tank through the feed port, the annular flexible light guide plate and the LED lamp which are arranged on the cylindrical reaction tank provide enough light sources for the circular microalgae reactor, and the flexible light guide plate has the advantages of high luminous efficiency and low power consumption under the condition of equal area luminance; the circular microalgae reactor provided by the utility model can fully utilize light, and the specific surface area of the circular microalgae reactor is higher than that of other microalgae reactors.
Absstract of: CN222524501U
The utility model relates to the technical field of microalgae culture, and discloses a columnar reactor system for microalgae culture, which comprises a columnar reactor body and a reflux pipeline, and one end of the reflux pipeline is connected to the bottom of the columnar reactor body. The other end of the backflow pipeline is communicated to a gas exchange space at the upper part of the columnar reactor body and is provided with a spray head, and a conveying pump is arranged on the backflow pipeline and is used for conveying the microalgae culture solution or the cleaning disinfectant in the columnar reactor body to the gas exchange space and spraying the microalgae culture solution or the cleaning disinfectant to the inner wall of the columnar reactor body through the spray head; according to the columnar reactor system provided by the utility model, the gas exchange space can be thoroughly cleaned and disinfected, and the problems that microalgae cells adhered to the side wall of the columnar reactor die due to the fact that the microalgae cells cannot be in contact with a microalgae culture solution for a long time and further pollute microalgae in the whole culture system are solved.
Absstract of: CN119506183A
The invention provides a method for regulating and controlling synthesis of microalgae fatty acid by using a long-chain carbon signal substance and application thereof, and the method for regulating and controlling synthesis of microalgae fatty acid by using the long-chain carbon signal substance comprises the following steps: culturing microalgae by using a culture medium to obtain a microalgae culture solution; then, a long-chain carbon signal substance is prepared into a solution, the long-chain carbon signal substance is C14-HSL, and the structural formula of the C14-HSL is shown in the formula I; adding a long-chain carbon signal substance solution into the microalgae culture solution to obtain a mixed solution; and culturing the mixed solution. According to the method, the C14-HSL signal substance is inoculated into the microalgae culture solution, so that the growth of microalgae can be effectively promoted, the relative content and composition of saturated fatty acid, monounsaturated fatty acid and polyunsaturated fatty acid in microalgae cells are influenced, the content of fatty acid related to biodiesel precursor synthesis is increased, and the yield and quality of biodiesel are improved. # imgabs0 #
Absstract of: CN119506047A
The invention discloses a photoconductive enhanced osmunda culture'light tree 'bioreactor for CO2 capture and resource utilization. The bioreactor comprises a transparent tank body made of a transparent material, and refractive fibers and hollow fibers are embedded in the transparent tank body. The refractive fiber is made of a high-light-conductivity material, can introduce and uniformly distribute an external light source into the whole bioreactor, and provides necessary illumination conditions for microalgae. The reactor can effectively capture carbon dioxide and utilize microalgae for resourceful culture. The reactor system provides a suitable microalgae growth environment with sufficient light and sufficient carbon sources for microalgae through gas-liquid separation, and promotes rapid growth of microalgae. The reactor can be applied to the fields of industrial waste gas treatment, greenhouse gas emission reduction, biological energy production, aquaculture wastewater and the like. A CO2 trapping technology is organically combined with a CO2 biosynthesis and resource utilization technology, so that efficient trapping and resource utilization of CO2 are realized.
Absstract of: CN119506094A
The invention relates to the field of microalgae breeding, in particular to a method for screening and cultivating yellow chlorella pyrenoidosa mutant strains capable of rapidly growing, which mainly comprises the following steps: marking wild type chlorella pyrenoidosa on a flat plate, selecting single algae colonies for activation culture, and culturing the single algae colonies to obtain the yellow chlorella pyrenoidosa mutant strains. The yellow chlorella pyrenoidosa capable of rapidly growing is screened and cultured, the yellow chlorella pyrenoidosa is cultured for 4-6 days under the heterotrophic condition of 25-30 DEG C, the color characterization is stable, the hereditary character of the yellow chlorella pyrenoidosa can be judged to be relatively stable, the growth rate is high, the chlorophyll content is remarkably lower than that of wild type chlorella pyrenoidosa, the biomass of the next day can reach 2.1 g/L under the condition that the optimal carbon source is 5g/L, and the yield is high. Compared with wild type chlorella pyrenoidosa, the invention has the advantages that the content of xanthophyll and biomass are higher than those of wild type chlorella pyrenoidosa, the amino acid composition is excellent, the EAA/TAA value is 44.74%, and the EAA/NEAA ratio is 0.81, so that the chlorella pyrenoidosa has the potential of replacing traditional edible resources, can be used as an optimal substitute of traditional food due to the excellent protein quality, and bro
Absstract of: CN119506046A
A high-efficiency self-luminous fiber'light tree 'bioreactor is characterized in that components of the bioreactor are provided with light guide fiber tubes, membrane filaments of a hollow fiber membrane are prepared by blending polymers and self-luminous materials, and the hollow fiber membrane has high light transmission to promote photosynthetic biological reaction. The reactor can effectively capture carbon dioxide and utilize microalgae for resourceful culture. The reactor system provides a suitable microalgae growth environment with sufficient light and sufficient carbon sources for microalgae through gas-liquid separation, and promotes rapid growth of microalgae. The reactor can be applied to the fields of industrial waste gas treatment, greenhouse gas emission reduction, biological energy production, aquaculture wastewater and the like. A CO2 trapping technology is organically combined with a CO2 biosynthesis and resource utilization technology, so that efficient trapping and resource utilization of CO2 are realized.
Absstract of: CN119506045A
The invention discloses a landscape illumination and outdoor algae ecological'light tree 'integrated bioreactor, which can provide illumination for algae by utilizing sunlight in the daytime, and can provide necessary illumination conditions for microalgae by utilizing artificial light sources such as street lamps and automobile light at night, and meanwhile, energy is saved. Through efficient photosynthesis, the reactor can remarkably absorb CO2 in the atmosphere, and greenhouse gas emission is reduced. The bioreactor further comprises a landscape lighting device, soft green light can be emitted at night by means of street lamps, the attractive landscape effect is formed, and the night visual attraction of a city is improved. The CO2 supply device ensures that the algae grow in a proper environment through CO2 absorbers arranged around the main body structure. The overall design not only has an environmental protection function, but also can be used as a part of urban landscape, enhances the aesthetic property and environmental awareness of cities, and is suitable for various outdoor environments such as urban green lands, parks and streets.
Absstract of: CN119506044A
The invention discloses a refractive braided tube hollow fiber membrane light tree bioreactor. The bioreactor comprises a transparent tank body made of a transparent material, the inner layer of a membrane wire is a refraction braided tube, the outer layer of the membrane wire is a polymer film with biological friendliness, and the refraction braided tube is used for introducing and uniformly distributing an external light source into the whole bioreactor to provide necessary illumination conditions for microalgae. The reactor can effectively capture carbon dioxide and utilize microalgae for resourceful culture. The reactor system provides a suitable microalgae growth environment with sufficient light and sufficient carbon sources for microalgae through gas-liquid separation, and promotes rapid growth of microalgae. The reactor can be applied to the fields of industrial waste gas treatment, greenhouse gas emission reduction, biological energy production, aquaculture wastewater and the like. A CO2 trapping technology is organically combined with a CO2 biosynthesis and resource utilization technology, so that efficient trapping and resource utilization of CO2 are realized.
Absstract of: CN119490944A
The invention relates to a microalgae culture method which comprises the following steps: culturing microalgae species in the presence of a fat-soluble additive to obtain a culture solution containing floating layer microalgae; and harvesting the microalgae on the floating layer. The fat-soluble additive is introduced in the microalgae culture process, the concentration of a microalgae floating layer can be effectively improved, the viscosity of the microalgae is improved, and therefore the culture efficiency and the harvesting efficiency of the microalgae are remarkably improved.
Absstract of: WO2025038756A1
Embodiments of the present disclosure pertain to a composition suitable for use in treating an agricultural material. In some embodiments, the composition includes a microalgae culture, an extract of the microalgae culture, compounds from the microalgae culture, or combinations thereof. Additional embodiments of the present disclosure pertain to methods of treating an agricultural material by applying a composition of the present disclosure to the agricultural material.
Absstract of: WO2023198689A1
The present invention relates to a process for obtaining a protein extract from microalgae. The protein extract obtained has a texturising ability to form a protein network, pure or in a mixture with another protein source, allowing the formation of food gels, extruded protein material or cooked cereal products. The protein extract obtained also has properties of coagulation, pure or in a mixture with another protein source, allowing the formation of food products that require protein coagulation without the formation of a protein network, such as hot emulsions or desserts ("custards"). The invention also relates to the use of the protein extract for replacing the texturising functionalities provided by animal proteins (egg proteins, dairy proteins, red or white meat proteins) within these products, and therefore for replacing sources of animal protein within these products.
Absstract of: WO2023198694A1
The present invention relates to a method for producing fermented textured microalgae, comprising steps of mixing and incubating the microalgae with lactic ferments, and also the composition obtained using the method, and the use of the composition for food.
Absstract of: WO2023201190A1
The subject invention provides compositions and methods for reducing deleterious atmospheric gases using microorganisms. In preferred embodiments, a composition comprising one or more beneficial microorganisms convert carbon dioxide to calcium carbonate, magnesium carbonate, a bicarbonate, or a combination thereof. The source of the carbon dioxide can be industrial emissions. In some embodiments, the composition sequesters carbon with the use of a trough growth system and the culture and/or growth by-product can be sequestered in underground geological formations.
Absstract of: WO2023198774A1
The invention provides a recombinant microalgal strain comprising in its genome a first modification which causes overexpression of a PSR1 gene, and optionally a further modification which reduces or eliminates expression from an endogenous PTC1 gene. The strains of the invention have utility in promoting phosphate uptake, for example from wastewater, with the microalgae then being useful as fertilisers.
Absstract of: CN119454566A
The invention relates to a biological oxygen therapy and antioxidation dual-strategy soluble microneedle as well as a preparation method and application thereof, and belongs to the technical field of medicines. The microneedle is an integrated soluble microneedle and comprises a matrix formed by a water-soluble polymer and a compound which is uniformly dispersed in the matrix and formed by microalgae and metal organic framework nano-enzyme, the compound formed by the microalgae and the metal organic framework nano-enzyme is a compound of a'core-shell 'structure, the metal organic framework nano-enzyme serving as the shell is wrapped on the surface of the microalgae serving as the core. The microneedle disclosed by the invention has the capability of rapidly resisting oxidation in situ and continuously and deeply conveying oxygen, is widely applied to preparation of medicine design or preparations for treating tumors, promoting wound healing, regulating an inflammation microenvironment, treating skin burns and scalds and removing scars, and is good in patient applicability; and the preparation process is green, environment-friendly, simple, rapid, mild in condition and suitable for large-scale production.
Absstract of: CN222499090U
The utility model relates to the technical field of microalgae photobioreactors, and discloses a slow flow thin layer type photobioreactor which comprises a supporting frame and a water tank container installed on the supporting frame, the water tank container is arranged in an inclined mode, and a plurality of baffles which are arranged in a staggered mode are arranged in the water tank container in an array mode in the extending direction. Through cooperation of the baffles which are arranged in the water tank container and are arranged in a staggered mode, the flowing track of algae liquid is in an S shape, the flowing path of the algae liquid is greatly increased, and the retention time of the algae liquid in the water tank container is prolonged; the flow velocity required for maintaining the algae liquid with the same thickness is obviously smaller than that required when the baffle plates are not arranged, so that the energy consumption caused by overhigh flow velocity can be reduced, and the energy consumption can be greatly reduced by adjusting the distance between the two adjacent baffle plates and the width of the water tank reactor.
Absstract of: CN222499138U
The pipeline type photobioreactor comprises a bottom plate and a top plate, a plurality of cleaning assemblies are arranged between the bottom plate and the top plate, a plurality of reaction straight pipes I and reaction straight pipes II are arranged on the front side and the rear side of each cleaning assembly respectively, the reaction straight pipes II are located on the rear sides of the reaction straight pipes I, and the reaction straight pipes II are located on the rear sides of the reaction straight pipes II. According to the pipeline type photobioreactor, the inner side of the spiral main body of the pipeline type photobioreactor can be fully cleaned by utilizing the cleaning assembly, and a cleaning worker does not need to extend a flushing pipe into a gap of a spiral pipeline when cleaning the inner side of the spiral reactor main body; and a user does not need to worry that the flushing pipe collides with the reaction straight pipe I, the reaction straight pipe II and the reaction curved pipe during cleaning, so that the pipeline type photobioreactor is more convenient and simpler to clean.
Absstract of: CN119464400A
The invention discloses a preparation method of microalgae nano-selenium and application of biotransformed nano-selenium in prevention and treatment of plant late blight. The novel preparation method of the microalgae nano-selenium comprises the following steps: firstly, extracting an intracellular extract of chlorella pyrenoidosa, and then adding sodium selenite into the intracellular extract to carry out nano-selenium conversion; compared with an existing reported method that after sodium selenite is added into a microalgae living body culture solution to be converted into nano-selenium, algae is taken to break cells, and the nano-selenium is collected, the method has the advantages that the microalgae living body does not need to be used when the nano-selenium is biologically converted, and the subsequent complicated nano-selenium separation and purification steps can be avoided; the nano-selenium obtained by converting the intracellular extract can be directly applied. Experiments prove that the effect of the nano-selenium obtained through biotransformation on inhibiting phytophthora infestans of potato late blight pathogenic bacteria is obviously better than that of chemically synthesized nano-selenium, and the nano-selenium obtained through biotransformation has an obvious effect on preventing and treating potato late blight and has an obvious effect on promoting plant growth.
Absstract of: CN119462252A
The invention discloses an extraction method of microalgae, and belongs to the technical field of microorganisms, and the extraction method comprises the three steps of microalgae culture, enzymolysis reaction and compounding aftertreatment. When a microalgae extract is applied to a microalgae fertilizer, the yield and quality of crops can be effectively improved; and the schizochytrium limacinum culture medium is treated by using p-hydroxybenzoic acid and diammonium phosphate, so that the cell membrane permeability of the schizochytrium limacinum is changed, other nutrient substances can enter the schizochytrium limacinum conveniently, and meanwhile, cell wall breaking of the schizochytrium limacinum is facilitated. Under a proper ratio of an enzymolysis mixture, pyrolytic biochar, bentonite and biomass tar, the prepared microalgae-biochar simultaneously has abundant pore structures, a large amount of microalgae loads and strong combination structural performance, the activity performance of the microalgae-biochar can be effectively maintained, and the application range of the microalgae-biochar is widened. The method has a wide market prospect when being used for preparing a microalgae fertilizer.
Absstract of: CN119464003A
The invention relates to a fish embryo-based high-concentration cyanobacteria toxin development toxicity evaluation device and method, and relates to the technical field of pollutant toxicity analysis, the device structure comprises a base and an electric guide rail mechanism 1; the carrier is arranged on the first electric guide rail mechanism in a sliding mode, a movable oscillation base is arranged on the carrier, a plurality of perforated plates used for placing fish embryos are arranged on the movable oscillation base, and a lighting structure is arranged at the position, located below the movable oscillation base, of the carrier; the liquid injection unit is used for injecting cyanobacteria toxin working solutions with different high concentrations into the porous plates, and an observation unit is arranged at the top of the incubation chamber; and the adjusting shading unit is arranged between the lighting structure and the perforated plate. The evaluation device provided by the invention is convenient to use, reduces the labor intensity of workers, improves the working efficiency, and facilitates large-scale and high-efficiency evaluation and analysis of the fish embryonic development toxicity of the high-concentration cyanobacteria toxin.
Nº publicación: CN119461667A 18/02/2025
Applicant:
NANJING UNIV OF SCIENCE AND TECHNOLOGY
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Absstract of: CN119461667A
The invention discloses a method for relieving microalgae high-temperature stress and improving sewage treatment performance by utilizing a ferroptosis inhibitor. According to the method, a ferroptosis inhibitor Fer-1 is added into a microalgae growth environment, so that Fer-1 fully enters microalgae cells, and then the ferptosis inhibitor Fer-1 is applied to sewage treatment. According to the method, the ferroptosis inhibitor Fer-1 is added to regulate and control the way of programmed cell death of the microalgae, so that the inhibition effect of high temperature on the growth of the microalgae is remarkably relieved, the balance of an intracellular oxidation-anti-oxidation system is effectively maintained, the influence of a lipid metabolism way of the chlorella is relieved, high-temperature stress resistance is realized, and the yield of the chlorella is improved. Meanwhile, the nitrogen and phosphorus removal performance of the sewage is improved, the removal rates of ammonia nitrogen and phosphorus are improved by 44.50% and 30.72% respectively, and the method has important application value for effectively protecting microalgae cells under the extreme temperature condition and promoting low-carbon resourceful treatment of the microalgae in the sewage.