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175
results.
Updated on
23/10/2026 [07:32:00]
Results 50 to 75 of 175
Absstract of: CN224646950U
The utility model relates to the technical field of microalgae culture biology, and discloses a microalgae in-situ reaction culture device which comprises a culture box body, a culture platform which is driven by a supporting shaft to rotate is arranged in the culture box body, and a clamping jig is arranged on the culture platform; a liquid supply end cover internally provided with a liquid supply cavity communicated with the liquid conveying pipe is fixed to the top of the incubator body, the upper portion of the supporting shaft is rotationally connected with the liquid supply end cover, a liquid conveying channel communicated with the liquid supply cavity is formed in the supporting shaft, and liquid distribution pipes which are communicated with the liquid conveying channel and correspond to the clamping jigs one to one are arranged on the outer side of the supporting shaft; according to the device, the uniformity of a culture environment is improved through rotation of the culture container, continuous liquid supply in a rotating state is realized, in-situ reaction can be completed without shutdown and box opening, environmental fluctuation and pollution risks are reduced, the reliability and repeatability of an experimental result are remarkably improved, and the problems of poor environmental uniformity and high experimental efficiency in the prior art are solved. And continuous liquid supply cannot be realized in a continuous rotating state of the culture container.
Absstract of: CN122581042A
The invention discloses a method for improving soil fertility of traditional Chinese medicinal materials by using a nitrogen-fixing cyanobacteria biological membrane. The method sequentially comprises six steps of blue-green algae culture, biological membrane matrix construction, biological membrane formation, biological membrane particle preparation, soil application and ecological regulation. The method comprises the following steps: screening blue-green algae strains with high nitrogen fixation activity, such as nostoc and anabaena, performing precise culture to obtain a high-activity culture solution, compounding porous minerals, an organic carbon source and a biopolymer adhesive into a biological membrane matrix, inoculating the blue-green algae culture solution, and regulating and controlling the environment to form a three-dimensional reticular blue-green algae biological membrane. The particles are applied to the 0-5 cm surface layer of the traditional Chinese medicine soil according to the application amount of 200-500 kg/hm < 2 >, so that the blue-green algae forms a biological crust structure. The soil structure is improved through cyanobacteria photosynthetic nitrogen fixation and exopolysaccharides, and a synergistic ecological system is formed with rhizosphere microorganisms.
Absstract of: CN122587839A
The invention discloses an environment-friendly microalgae culture pond, and belongs to the technical field of microalgae culture, the environment-friendly microalgae culture pond comprises a pond body, a partition bin is arranged in the middle of the pond body, the bottom of the partition bin is fixedly connected with the inner wall of the pond body, and the side wall of the pond body is provided with a driving mechanism used for driving the interior of the pond body to flow circularly. A light guide mechanism used for guiding light into the bottom layer of water flow in the pool body is arranged in the partition bin, and cleaning mechanisms used for cleaning the output end of the light guide mechanism and driven by a driving mechanism in a linkage mode are arranged on the two sides of the partition bin. An external light source is transmitted to the bottom layer of the pond body through the light guide mechanism via the light collecting cover and the light guide cylinder on the inner wall of the reflecting layer, so that the problem of insufficient illumination of the bottom layer caused by water depth of a traditional microalgae culture pond is effectively solved; and the transmission structure is linked with the cleaning mechanism to periodically and automatically clean the diffuse reflection light-transmitting piece, so that the situation that the light transmittance is affected by dirt attachment is prevented.
Absstract of: CN122587877A
The invention discloses a microalgae culture solution and a culture method. According to the microalgae culture solution, microalgae is scenedesmus or chlorella, and the formula of the microalgae culture solution is as follows: 0.4-1.2 g of yeast extract powder and 1 liter of water. The culture method of the microalgae culture solution comprises the following steps: preparing the culture solution according to the formula, and filling the culture solution into a container; inoculating algae species in the growing period according to the proportion of 5-20%, and carrying out aeration/stirring culture; performing closed or semi-open or full-open culture for 3-7 days; and putting the cultured algae seeds into a water body needing to be purified or storing the algae seeds in a shady, cool and ventilated place for later use after filling. The technical scheme solves the technical problems of simplifying the formula components of the culture solution, simplifying the operation, promoting the growth, reducing the cost and reducing the requirements on preparation technicians.
Absstract of: CN122586263A
The invention relates to the technical field of water pollution treatment and environmental bioremediation, in particular to a method for removing heavy metals in a water body by using organic acid enhanced microalgae. The method comprises the following steps: inoculating microalgae in a logarithmic phase into a heavy metal polluted water body, then adding organic acid, culturing the water body, and carrying out solid-liquid separation to remove the heavy metals in the water body; the microalgae is scenedesmus quadricauda; and the organic acid is citric acid. According to the method, the synergistic effect of extracellular chelating detoxification of citric acid and intracellular enrichment optimization of microalgae is utilized. The citric acid not only reduces the acute toxicity of free-state metal ions, but also improves the exposure degree of functional groups on the surfaces of cells, so that the surface adsorption quantity and intracellular accumulation quantity of the microalgae to Cu and Zn reach peak values, and the synergistic removal efficiency of heavy metals is greatly improved.
Absstract of: CN122588203A
The invention discloses a screening method and application of a symbiotic strain of coal gangue decomposing bacteria and salt-tolerant microalgae, and relates to the technical field of environmental microorganism and waste resource utilization. The method comprises the steps of liquid-phase primary screening and solid-phase secondary screening, wherein in the liquid-phase primary screening, the algae density serves as an index, and strains are divided into growth promotion (larger than or equal to 1.55 times), microsymbiosis (smaller than or equal to 5% fluctuation) or algae inhibition (decreasing gt, 10%); and the growth-promoting and micro-symbiotic strains are retained. Coal gangue is used as a substrate for solid-phase secondary screening, blank and single-bacterium/single-algae control are set, and screening standards are as follows: the effective nutrient of a phycomycete co-treatment group is increased remarkably higher than the sum of single groups, the pH change is within +/-0.5, the final value is 6.0-8.0, and the ratio of exopolysaccharides is gt; and 50%. The screened symbiotic system has the functions of growth promotion, improvement and acidification resistance, and can be used for coal gangue improvement and ecological restoration.
Absstract of: CN122587878A
The invention belongs to the technical field of microorganisms, and particularly relates to micro-miscanthus XY7 with a function of synergistically enriching organic selenium, nano-selenium and carotenoid and application of the micro-miscanthus XY7. The microcystis spp XY7 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number of the microcystis spp XY7 is CCTCC NO: M 20252714, and the preservation date of the microcystis spp XY7 is November 27, 2025. The invention provides a novel high-selenium-resistant microalgae strain microawn XY7, and the tolerance concentration of the novel high-selenium-resistant microalgae strain microawn XY7 to inorganic selenium ions reaches 2000 mg.L <-1 >. According to the method, the micro-miscanthus XY7 can normally grow and breed in the culture medium with high selenium concentration, high-selenium raw materials or selenium-containing wastewater do not need to be diluted, the raw material cost and the pretreatment cost of selenium-rich microalgae culture are greatly reduced, and meanwhile, excellent germplasm resources are provided for bioremediation of high-selenium polluted water and soil.
Absstract of: CN122588197A
The invention discloses a dunaliella salina autophagy level evaluation method based on rapamycin induction and monodansyl cadaverine fluorescence detection. The method comprises the following steps: culturing dunaliella salina to a logarithmic phase, adding a sample, and continuously culturing for 12-24 hours; monodansyl cadaverine is added for dyeing; the method comprises the following steps: a) determining the fluorescence intensity by using a fluorospectro photometer; b) observing the form, the number and the subcellular distribution of the autophagosome by using a laser scanning confocal microscope; or c) determining the average fluorescence intensity or the percentage of positive cells in an FL1 channel by using a flow cytometer. And judging the autophagy-induced activity level of the sample according to the difference between the sample group and the control group. A quantitative autophagy detection system suitable for dunaliella salina is systematically established for the first time, the method has the advantages of being easy and convenient to operate, rapid in detection, objective in result, capable of being applied in a high-throughput mode and the like, and a standardized technical tool is provided for microalgae autophagy mechanism research and stress resistance breeding.
Absstract of: CN122556267A
The invention discloses an artificial cyanobacteria crust constructed by utilizing pullulan and EPS-producing bacteria and a preparation method of the artificial cyanobacteria crust, and belongs to the technical field of ecological restoration and desertification control. The method comprises the following steps: preparing a mixed soil algae culture solution and a high-yield EPS bacterial suspension, and jointly inoculating a pullulan solution, the algae culture solution and the bacterial suspension on the surface of soil for culturing to form the artificial cyanobacteria crust. The pulullan adopted by the invention has good film-forming and binding properties, and can enhance the binding force among soil particles in a short time; the EPS-producing bacteria can continuously secrete exopolysaccharides, a three-dimensional network structure is formed among soil particles and on the surfaces of microorganisms, and the mechanical strength, wind erosion resistance and disturbance resistance of the crust are remarkably improved; the extracellular polysaccharide secreted by the EPS-producing bacteria has excellent water retention performance, and the survival rate and the ecological function of the whole biological crust system are improved; the artificial cyanobacteria crust is rapidly formed by regulating the concentration of pullulan and the variety and proportion of EPS-producing bacteria and algae.
Absstract of: CN122563594A
The invention relates to a biochar-biogas slurry-microalgae composite material based on nano CO2 bubble regulation and control and application of the biochar-biogas slurry-microalgae composite material in promotion of soil biological crust. According to the method, accurate pH regulation and control and CO2 enrichment are realized through two times of nano CO2 bubble injection: the first time is used for acidification and carbon enrichment of a biochar-biogas slurry mixed system; and for the second time, activation and pH fine adjustment of the material before spraying are carried out, so that the pH of the system is reduced to be below 7, and the microalgae activity and EPS secretion capacity are enhanced. Through a quadruple synergistic mechanism of nano CO2 bubble regulation and control, biochar skeleton support, biogas slurry nutrition supply and microalgae EPS bonding, rapid surface skinning, wind erosion resistance enhancement, evaporation inhibition and carbon sequestration increase in the cold and arid region are realized.
Absstract of: CN122563852A
The invention discloses application of polygonatum sibiricum leach liquor in promotion of growth of chlorella, and belongs to the technical field of chlorella cultivation. According to the method, the rhizoma polygonati extract and the chlorella culture solution are mixed and then cultured, the growth of the chlorella can be effectively promoted, a natural plant source extract is adopted to replace a traditional chemical additive or an organic carbon source, the problems of poor environmental compatibility and high production cost caused by chemical substances are avoided, and the method is suitable for industrial production. And a green and low-cost growth promoting mode is provided for large-scale culture of chlorella. Besides, the algae biomass obtained by culturing the chlorella containing the polygonatum sibiricum extract can be used as a natural delivery carrier of active ingredients of polygonatum sibiricum and can be used for producing functional foods or cosmetics, so that the synergistic utilization of microalgae culture and traditional Chinese medicine resource development is realized.
Absstract of: CN122562149A
The invention discloses a preparation method of magnetic aldehyde cationic starch and a method for collecting microalgae through flocculation under the assistance of an external magnetic field, and relates to a preparation method of a flocculant and a method for collecting microalgae. The invention aims to solve the technical problems that the separation speed is slow when the cationic starch is used for flocculating microalgae, floc is easy to break under high disturbance, and the economical efficiency is poor due to the fact that the required dosage is too high in the prior art. The magnetic aldehyde group cationic starch is obtained by carrying out cationization modification on natural starch and then introducing an aldehyde group functional group and FeOmagnetic nanoparticles. The flocculating agent is added into a microalgae-containing water body, and rapid mixing and sedimentation treatment are performed under the action of an external electric field, so that rapid aggregation and efficient solid-liquid separation of microalgae cells can be realized, and the microalgae recovery rate of 85.1% can be achieved within 5 minutes. The method can be used in the fields of water treatment and microalgae resource utilization.
Absstract of: CN122563851A
The invention relates to a microalgae carbon sequestration technology, and aims to provide a method for improving the protein content and calcium and magnesium ion absorption capacity of microalgae through high-salt domestication. Comprising the following steps: inoculating chlorella cells into a basic culture medium, and then carrying out high-salt gradient domestication under the condition of introducing CO2-containing flue gas; the content of calcium and magnesium ions in the culture system is synchronously increased in each domestication stage, and the same concentration is increased each time until the final concentrations of the calcium and magnesium ions in the final culture system are 5g/L respectively; screening the chlorella communities subjected to high-salt gradient domestication to obtain algae strains with the highest protein content and good growth; and carrying out transfer culture on the screened algal strains, and verifying the genetic stability of high protein content and high calcium and magnesium ion absorption performance. The carbon sequestration chlorella with significantly improved protein content can be obtained only through one-time domestication and screening, and the nutritional value and economical efficiency of the frond biomass as feed, food and biological product raw materials are improved.
Absstract of: CN122563695A
The invention discloses a microalgae self-adaptive culture system and a culture regulation and control method, the culture system comprises a reactor assembly, a photoelectric coupling assembly and an intelligent control assembly, the reactor assembly is provided with a containing cavity for containing a microalgae culture solution; the photoelectric coupling assembly is coaxially sleeved and fixed outside the reactor assembly, the photoelectric coupling assembly comprises a light emitting unit and a spectrum acquisition unit, and the light emitting unit and the spectrum acquisition unit are located on the inner ring surface of the photoelectric coupling assembly and are arranged in a staggered manner in the circumferential direction; the light emitting surface of the light emitting unit and the receiving surface of the spectrum acquisition unit face the accommodating cavity; the intelligent control assembly is in signal connection with the light-emitting unit and the spectrum acquisition unit. The technical problems that in existing high-density microalgae culture, light inhibition early warning lags behind, and the light energy utilization rate is low are solved.
Absstract of: CN122562187A
The invention relates to the technical field of sewage treatment, and discloses a microalgae synergistic pollution reduction and carbon reduction treatment method for dairy farm sewage. The method comprises the following steps: firstly, feeding dairy farm sewage subjected to pretreatment and anaerobic fermentation into a microalgae photosynthetic reaction unit, and identifying an inflow water quality fluctuation event in combination with inflow water quality data and dairy farm production period information; profile data of illumination, turbidity, dissolved oxygen, pH and temperature are collected in the water depth direction, and the position and thickness of an effective photosynthetic water layer are determined; the liquid level depth and the circulating stirring frequency are adjusted according to the change of the effective photosynthetic water layer, and the carbon dioxide injection flow and the hydraulic retention time are adjusted in a linkage mode; recording water quality, energy consumption, equipment operation and carbon dioxide injection states in the effluent discharge and microalgae harvesting process, and executing degraded operation or parameter backspacing according to abnormal grades; according to the invention, the effluent stability, the carbon dioxide utilization rate and the pollution reduction and carbon reduction operation reliability of dairy farm sewage treatment can be improved.
Absstract of: CN122556421A
The invention relates to a fishery ecological breeding system and a breeding method. The system comprises a prawn breeding pond, a settling pond, a multi-stage biological tail water purification device, a microfilter, a medium-pressure ultraviolet sterilizer, an algae breeding pond, a shellfish three-dimensional soilless breeding device and a tail water reservoir which are sequentially connected through a circulating pipeline. The prawn culture tail water is subjected to solid-liquid separation through a sedimentation tank and then enters a multi-stage biological tail water purification device, and nitrogen and phosphorus are synergistically removed by utilizing microalgae and bacteria; purified water is filtered and disinfected through a microfilter and a medium-pressure ultraviolet sterilizer and then enters a microalgae culture pond, and tail water serves as a culture medium to produce high-concentration algae liquid such as cyclotella and isochrysis galbana; algae liquid is used for three-dimensional culture of shellfish for filter feeding, excrement and residual feed are discharged to the sedimentation tank, and supernatant effluent flows back to the prawn culture pond to form a closed loop. According to the method, ecological coupling of shrimps, algae and shellfish can be achieved, near-zero emission of breeding tail water is achieved, shellfish products are free of mud smell, the comprehensive cost is greatly reduced, and the method is particularly suitable for factor
Absstract of: CN122563737A
The invention provides a staggered cultivation method of spirulina and haematococcus pluvialis, which comprises the following steps: in four seasons of one year, two microalgae such as spirulina and haematococcus pluvialis are cultivated in three types of devices such as a greenhouse raceway pond, a flat plate type photobioreactor and a pipeline type photobioreactor in staggered adverse periods; spirulina is cultivated in a greenhouse raceway pond and stored in a plastic barrel, and full-period cultivation of the spirulina in the greenhouse raceway pond can be achieved. According to the staggered-time cultivation method for haematococcus pluvialis flat plate type photobioreactor green nutrition cell cultivation, pipeline type photobioreactor erythrocyte transformation cultivation, greenhouse raceway pond green nutrition cell cultivation and fixed cell cross-season preservation, four-season continuous cultivation of haematococcus pluvialis can be achieved. According to the method, light, temperature, time and space resources are fully utilized, the productivity of the two microalgae is reasonably adjusted, and time-staggered efficient cultivation is achieved.
Absstract of: US20260234544A1
This microorganism belongs to the genus Rhodococcus, Xanthobacter, Ancylobacter, Shewanella, or Aeromonas. Said microorganism may be Rhodococcus cerastii belonging to the genus Rhodococcus, Xanthobacter flavus belonging to the genus Xanthobacter, or Ancylobacter rudongensis belonging to the genus Ancylobacter. Said microorganism may be for promoting the growth of cyanobacterium, green algae, glaucophyte algae, or Euglena algae. Said microorganism may be for promoting the growth of organisms belonging to the phylum Cyanobacteria, Ochrophyta, Euglena, Cryptophyta, Haptophyta, Cercozoa, Glaucophyta, Rhodophyta, Chlorophyta, or Streptophyta.
Absstract of: EP4790131A1
A process for producing calcium hydroxide, comprising:reacting calcium carbonate with an acid in an aqueous solution at a temperature of 10-50°C to produce a calcium ion solution and carbon dioxide,separating the carbon dioxide from the calcium ion solution,treating the calcium ion solution to form calcium hydroxide,directing the separated carbon dioxide to a photobioreactor containing microalgae, andgrowing the microalgae in the photobioreactor using the carbon dioxide through photosynthesis.
Absstract of: EP4790127A1
A system and method are provided for processing an industrial effluent and cultivating microalgae. The system comprises a primary tank, a screening unit with a motor-driven cleaning mechanism, a flocculation tank supplied with a chemical flocculant, a sand filter, an acidification unit with pH control, a solid-liquid separation unit, an anaerobic digestion unit, and a microalga cultivation unit. The effluent is screened, flocculated, filtered, acidified to a pH from 4.2 to 5.2, preferably 4.45 to 4.75, and subjected to solid-liquid separation to remove precipitated proteins and destabilised colloidal material, thereby producing a clarified effluent. The clarified effluent is anaerobically digested at a defined organic loading rate to generate biogas and a liquid digestate. At least a portion of the digestate and/or carbon dioxide derived from the biogas is used for microalga cultivation. The system enables stable anaerobic digestion without membrane-based pretreatment and provides a conditioned stream suitable for downstream microalga growth.
Absstract of: CN122542608A
The invention discloses a method for efficiently synthesizing human selenoprotein P from microalgae, and belongs to the field of bioengineering. Aiming at the problem that human selenoprotein P contains 10 selenocysteines and the translation readthrough efficiency is low due to multiple UGA termination codons in heterologous expression, the method comprises the following steps: performing codon optimization on a nucleotide sequence for coding the human selenoprotein P according to the codon preference of chlamydomonas reinhardtii; the method comprises the following steps: constructing a multi-copy expression vector containing two tandem expression cassettes, wherein each expression cassette is composed of an HSP70A-RBCS2 strong promoter, an optimized target gene and a terminator; transforming chlamydomonas reinhardtii, and screening a recombinant algal strain; culturing, adding a selenium source to induce expression, collecting algae strains, and purifying. Complete sequence expression of the human selenoprotein P containing 10 selenocysteine is realized in the chlamydomonas reinhardtii for the first time, the obtained protein has correct Sec doping and glycosylation modification, and the chlamydomonas reinhardtii is low in culture cost, free of endotoxin and suitable for production of food-grade or pharmaceutical-grade recombinant protein.
Absstract of: CN122542385A
The invention belongs to the technical field of gene engineering and biology, and particularly relates to a Myrmecia incisa MLDP gene knockout mutant strain as well as a construction method and application thereof. The MLDP gene knockout mutant strain (delta MiMLDP) of Myrmecia incisa is obtained by performing targeted knockout on the MLDP gene of Myrmecia incisa through a CRISPR/Cas9 gene editing technology. Compared with a wild type, the obtained MLDP gene knockout mutant strain has a remarkably changed grease accumulation phenotype, can be used for grease production and biofuel preparation, can also be used for feed preparation, and can also be used as an additive or a carrier for preparation of food, cosmetics and medicines. Targeted knockout of the MLDP gene is achieved in Myrmecia incisa for the first time, a genetic material is provided for studying the function of MLDP in microalgae grease metabolism, and a new germplasm is also provided for creating microalgae with novel grease composition and lipid droplet structures.
Absstract of: CN122542346A
The invention discloses a device and a method for clean ecological cultivation of haematococcus pluvialis by an outdoor photobioreactor, and relates to the technical field of artificial cultivation of microalgae, and the method comprises the following steps: forming an annular raceway pond, creating an outdoor dust-free clean cultivation environment, arranging a partition plate, placing bundled waste glass tubes, and arranging a screen; a solid arc-shaped platform is arranged, alternanthera philoxeroides is planted, oocystis bogei is introduced for cultivation, and the oocystis bogei and zooplankton in the oocystis bogei form a food chain system; planktonic animals and plants and alternanthera philoxeroides in the water body are fully utilized by the crayfish, and waste generated by the crayfish enters the water body of the raceway pond to be recycled through material and energy circulation. The clean and ecological cultivation method is utilized, raised dust is reduced, the local light and temperature environment is improved, the influence of periodic breeding of various animals and plants on the land on microalgae production is reduced, large-scale efficient haematococcus pluvialis cultivation and crayfish cultivation integrated production is achieved, and the space utilization rate is increased.
Absstract of: CN122541024A
The invention provides a bacteria-algae symbiotic artificial wetland system and an operation method, and relates to the field of rainwater treatment and resource utilization. The algal-bacterial symbiotic artificial wetland system comprises a wetland bed body, a water inlet device, a water drainage device and a control unit. According to the system, an up-down partition structure and dry-wet alternate operation are adopted, the impact load resistance is effectively improved, and the energy consumption of the system is remarkably reduced through natural drying, emptying and reoxygenation. The algal-bacterial microspheres in the light-transmitting area avoid flora loss, in-situ oxygen production of microalgae supports symbiotic bacteria to realize high-efficiency denitrification, and low-carbon and stable purification of multi-element pollutants in complex runoff is finally realized by combining the synergistic interception effect of the filler at the bottom layer of the non-light-transmitting area.
Nº publicación: CN122542368A 11/08/2026
Applicant:
TONGWEI AGRICULTURAL DEV CO LTD
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Absstract of: CN122542368A
The invention discloses an automatic high-photosynthetic-efficiency microalgae reactor and application thereof, and relates to the technical field of microalgae reactors, the automatic high-photosynthetic-efficiency microalgae reactor comprises a reactor support assembly, a plurality of conveyor belt gear shafts are rotatably arranged at the upper end and the lower end of the reactor support assembly, and the conveyor belt gear shafts are connected with a solid attachment medium through a transmission assembly; a nutrient solution supply assembly is arranged at a gear shaft of a conveying belt arranged at the upper end, a transmission assembly drives a solid attachment medium to sequentially flow through an inoculation area, an illumination culture area, a microalgae harvesting area, a cleaning and disinfecting area and an inoculation area, and a closed circulation loop is formed, so that automatic continuous production is realized; the adjacent conveyor belt gear shafts which are arranged in a vertically staggered mode enable solid attachment media to form inclined sections between the conveyor belt gear shafts, the multiple inclined sections are combined to enable the overall path of the media to be in a W-shaped winding shape, the microalgae attachment area on the unit occupied area is increased while microalgae attachment is guaranteed, and the yield is increased.
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