Absstract of: WO2026129604A1
The present invention relates to the field of microbial fertilizer manufacturing. Disclosed are an in-situ manufacturing and application device and method for an agricultural microalgae fertilizer. The device comprises a first box, wherein the first box is filled with a culture solution and microalgae; a replenishment tube is in communication with the first box; a plurality of small overflow holes are provided in a side wall of the first box; the replenishment tube can replenish the culture solution in the first box; when the replenishment tube injects the culture solution into the first box and the liquid level of the culture solution reaches the small overflow holes, culture solution containing microalgae flows out into the soil; and when the injection of the culture solution into the first box is stopped, the microalgae remaining in the first box continue to grow.
Absstract of: CN122266461A
The invention discloses an intelligent regulation and control method and system for chlorella high-nitrogen and high-phosphorus wastewater treatment based on WGCNA and machine learning, and belongs to the technical field of wastewater treatment. The method comprises the following steps: constructing a chlorella high nitrogen and phosphorus wastewater treatment time sequence experimental system, and synchronously collecting physiological phenotypes and whole transcriptome sequencing data of multiple time nodes; the method comprises the following steps: constructing a gene co-expression network by adopting WGCNA, and screening a feature module and a candidate gene set which are remarkably related to target traits of wastewater treatment; constructing a working condition classification and phenotype prediction model in combination with a random forest algorithm; integrating two types of analysis results to screen a core hub gene set, constructing a gene marker, and realizing early prediction of wastewater treatment efficiency and intelligent process regulation and control. According to the method, the chlorella wastewater treatment efficiency can be accurately predicted, the high-nitrogen and high-phosphorus wastewater treatment efficiency is remarkably improved, and theoretical support and a complete technical scheme are provided for a microalgae wastewater recycling technology.
Absstract of: CN122256151A
Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding a xylose isomerase, where the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganism comprises at least one nucleic acid sequence encoding a xylulokinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganism is capable of growing on xylose as a carbon source.
Absstract of: CN122254677A
The invention provides a cascade coupling pretreatment and microalgae recycling method for high-ammonia-nitrogen wastewater. The method comprises the steps of ammonia nitrogen pre-evaluation and air stripping, weak acid regulation and aeration carbon removal, enhanced acidification and acid coagulation, ozone oxidation and foam separation, pH back regulation and microalgae culture. According to the technical scheme, through multi-stage cooperation of decarburization, acidification and oxidation, a carbonate buffer system in the wastewater is broken through aeration under the weak acid condition, and follow-up acid consumption is greatly reduced; contaminants are separated out through strong acid coagulation; and finally, low-cost and high-efficiency pretreatment of the wastewater is realized by utilizing ozone oxidation deep purification and foam separation. Nutrient elements of the treated wastewater are well reserved, the pH value is stable, and the wastewater can be efficiently converted into microalgae biomass after being inoculated with microalgae with high ammonia nitrogen tolerance. The problems that in the prior art, high-ammonia-nitrogen and high-alkalinity wastewater treatment cost is high, decoloration and turbidity reduction are difficult, ammonia nitrogen toxicity inhibition and sterilization pH are out of control and the like are solved, and resource utilization and energy utilization of wastewater are achieved.
Absstract of: CN122251299A
The invention discloses a shrubalthea flower composite extract as well as a preparation method and application thereof. The compound extract is prepared from shrub althea flower exosome, shrub althea flower traditional active ingredients, edelweiss extract and microalgae exopolysaccharides, wherein the shrub althea flower exosome and the shrub althea flower traditional active ingredients are extracted from shrub althea flower raw materials at the same time through an integrated extraction process. The preparation method comprises the steps of raw material pretreatment, integrated extraction, separation and purification, synergistic compounding and post-treatment, and two types of active substances are efficiently and synchronously obtained by optimizing solvent, temperature, time and purification parameters. The compound extract shows excellent anti-aging, anti-inflammatory and moisturizing effects in cosmetics, and has excellent effects of regulating intestinal flora and enhancing immunity in functional food. According to the process, a remarkable synergistic effect is generated by combining specific synergistic components, the effect is far better than that of a single component or a simple mixture, and maximum utilization of plant resources and synergistic improvement of effects are achieved.
Absstract of: KR20260095297A
본 발명은 단일 종의 미세조류를 대량을 배양할 수 있는 자동화된 대량 배양 방법 및 그 시스템에 관한 것이다. 구체적으로는 수평으로 배치되며 서로 연결된 복수의 배양기가 다단으로 배치되어 있고, 각 단의 첫 번째 배양기들은 서로 연결되어 있는 복수의 배양기를 이용하여 미세조류의 추가 접종 없이도 대량으로 미세조류를 생산할 수 있는 대량 생산 방법 및 그 시스템에 관한 것이다.
Absstract of: CN122256327A
The invention discloses a preparation method and application of a filamentous microalgae and biochar compound fertilizer for phosphorus synergy of saline-alkali soil, and belongs to the field of agricultural resource utilization. Through a three-step synergistic process of acid modification, ultrasonic assistance and co-culture loading, stable colonization and function enhancement of the microalgae in the porous structure of the charcoal are realized. Organic acid is creatively adopted to modify biochar, the pore structure and surface functional groups of the biochar are optimized, and the adhesion capacity of microalgae is improved; then, pores of the biochar are cleaned through ultrasonic assistance, and microalgae cells are promoted to enter the pores; and finally, co-culturing the modified charcoal and filamentous microalgae under an illumination condition, so that the microalgae actively grows and is colonized in a porous structure of the charcoal, and the integrated active microalgae-charcoal compound fertilizer is formed. The soil conditioner has the advantages of increasing the contents of available nitrogen and available potassium in soil, remarkably improving the bioavailability of original phosphorus in the soil and the like. In addition, the soil can be adjusted to be in a neutral range, and a healthy and long-acting soil micro-ecological system is constructed.
Absstract of: CN224377813U
The utility model provides a full-circulation fishery breeding water treatment device based on photocatalytic microalgae coupling, which mainly comprises a photocatalytic treatment pond, an adult breeding pond, a seedling breeding pond and a microalgae bait breeding pond, an anode, a cathode, a photocatalytic film, deposited bottom mud, an ultraviolet lamp, a CO2 aeration disc, a gate valve, a water pump, an LED light source and a water quality sensor are also arranged in different tanks. An anode, a cathode, a water body and an external circuit in the water treatment device jointly form a bacteria-algae biological fuel cell for supplying power to equipment such as an ultraviolet lamp of the photocatalytic treatment pond, an LED light source of each culture pond, a water pump and the like; a water quality sensor network is formed in the full-circulation water treatment device, and the processes of water supplementing, bait supplementing and the like are efficiently controlled in time. According to the utility model, the photocatalytic oxidation and the biological fuel cell are cooperated to purify culture sewage, the microalgae is used as bait to reduce cost and improve immunity, and the microbial fuel cell generates power to supply oxygen to the microalgae light source, so that zero emission, full circulation and water saving are realized.
Absstract of: CN122235188A
The invention relates to a construction method of a blue algae engineering strain capable of improving photosynthesis efficiency. The construction method comprises the following steps: constructing a recombinant vector pUC18-NS17942-GmR-pCPCB-HmaS *-HAOX2-GSH containing a mutant 4-hydroxymandelic acid synthase HmaS * coding gene, a hydroxy acid oxidase HAOX2 coding gene, glutathione peroxidase and a glutathione reductase GSH coding gene; the recombinant vector pUC18-NS17942-GmR-pCPCB-HmaS *-HAOX2-GSH is integrated to a genome neutral site NS1 of a cyanobacteria host, and the cyanobacteria engineering strain is obtained through gentamicin resistance screening. According to the method, the proportion of O2/CO2 in cells is directly adjusted through a metabolic engineering means, the RuBisCO carboxylation efficiency is remarkably improved on the premise that RuBisCO is not modified, and finally stable increase of photosynthetic efficiency and biomass is achieved.
Absstract of: CN224378023U
The utility model discloses a multilayer spiral photobioreactor and relates to the field of bioreactors. The multilayer spiral photobioreactor comprises a base, and further comprises a transparent tank body fixedly connected to the base; the sealing cover is detachably connected to the tank body; the infusion pump is arranged on one side of the base; one end of the liquid conveying pipe is communicated with the interior of the lower end of the tank body; the transparent tank body and the spiral pipe guarantee natural light penetration, the LED lamp bar accurately supplements light, the spiral path prolongs the light receiving time of algae liquid, the problems that a traditional reactor is uneven in illumination and insufficient in light receiving are solved, the photosynthetic efficiency of microalgae is promoted to be improved, and the microalgae growth rate is directly increased, and the biomass accumulation is increased; the microalgae are in full contact with nutrient substances and light through the combination of algae liquid circulating flow and gas microbubble injection, algae liquid layering and local nutrient deficiency are avoided, the stability of the microalgae growth environment is guaranteed, and the culture consistency and yield are improved.
Absstract of: CN224371095U
The utility model discloses a gas-liquid countercurrent contact purification tower and relates to the field of waste gas treatment. The gas-liquid countercurrent contact purification tower comprises a transparent tower body, the lower end of one side of the transparent tower body is fixedly communicated with a gas conveying pipe, the upper end of the transparent tower body is fixedly communicated with an exhaust pipe, and the gas-liquid countercurrent contact purification tower further comprises a spraying pipe fixedly connected to the upper end of the interior of the transparent tower body; according to the utility model, a countercurrent mode of feeding CO2-containing waste gas from the bottom and spraying algae liquid from the top is adopted, and a plurality of filler layers with immobilized algae species are matched, so that the gas-liquid contact time is greatly prolonged, the mass transfer is enhanced, the problems of short contact between microalgae and pollution gas and low carbon sequestration efficiency are solved, the waste gas is efficiently purified, the concentration of the algae liquid is stabilized by a tower bottom circulating pump, and continuous and stable contact is ensured; the cylindrical structure is matched with multiple layers of fillers, the occupied space is small, the scene with limited space is adapted, the transparent tower body can visually monitor the internal gas-liquid and algae-liquid states, the abnormity can be timely handled, and the oper
Absstract of: CN122235189A
The invention relates to a construction method of a blue-green algae engineering strain for improving photosynthetic efficiency based on tyrosine metabolism. The method comprises the following steps: constructing a recombinant vector pUC18-NS17942-GmR-pCPCB-Hpd-HmgABC containing a 4-hydroxyphenylpyruvate dioxygenase (Hpd) encoding gene, a homomelanic acid dioxygenase (HmgA) encoding gene, a fumaryl acetoacetic acid hydrolase (HmgB) encoding gene and a maleoyl acetoacetic acid isomerase (HmgC) encoding gene; the recombinant vector pUC18-NS17942-GmR-pCPCB-Hpd-HmgABC is integrated to a genome neutral site NS1 of a cyanobacteria host, and the cyanobacteria engineering strain is obtained through gentamicin resistance screening. According to the method, the proportion of O2/CO2 in cells is adjusted by constructing an oxygen removal metabolism module, the RuBisCO carboxylation efficiency is remarkably improved on the premise that RuBisCO is not modified, and finally stable increase of photosynthetic efficiency and biomass is achieved.
Absstract of: CN122228956A
The invention discloses a biological bait fortification method for increasing the survival rate of octopus ocellatus larvae and promoting growth, which is applied to biological bait in the stage (from early hatching to bottom attaching stage, and suitable for trunk length of 0.5 cm-1. 5cm) of the octopus ocellatus larvae, and comprises the following steps: screening out an optimal microalgae ratio and fortification time, and carrying out nutrition fortification on disinfected living bait, so that the survival rate of the octopus ocellatus larvae is increased, and the survival rate of the octopus ocellatus larvae is increased. The method has the advantages that diseases of octopus ocellatus due to ingestion of baits with germs are prevented, nutritional components of biological baits are specially made through a food chain according to growth and development requirements of the octopus ocellatus, the survival rate and digestive gland index of the young octopus ocellatus are increased, the development cycle of the young octopus ocellatus is shortened, and good growth and development of the octopus ocellatus are guaranteed; the polyunsaturated fatty acid provided by the invention is a key nutrient substance for the development of the octopus ocellatus larvae, and a flowing mode of key nutrient components including DHA, EPA, ARA and essential amino acids is disclosed, so that the method has important significance on large-scale artificial breeding of the octopus ocellatus and dir
Absstract of: CN122233588A
The invention discloses a low-carbon emission treatment method for aquaculture tail water, which comprises the following steps: carrying out electrochemical oxidation and flocculation treatment on the aquaculture tail water through an electrochemical reactor, converting macromolecular organic carbon into micromolecular volatile fatty acid, and simultaneously forming a composite floc and treated effluent; the treated effluent enters a biofloc reactor, volatile fatty acid is used as a carbon source, dissolved nitrogen and phosphorus are converted into mycoprotein and polyphosphate through microbial assimilation, and biofloc and biochemical treated effluent are formed; the biochemical treatment effluent is introduced into a photobioreactor, residual inorganic nitrogen and inorganic phosphorus are absorbed and carbon dioxide is fixed by utilizing microalgae photosynthesis, and microalgae biomass and purified effluent are produced; and mixing the biological floc and the microalgae biomass to prepare a recycling product. The method is used for solving the technical problems that organic carbon and nitrogen and phosphorus resources in tail water are used as wastes for high-energy-consumption degradation and are not used as recyclable resources, and the recycling value of the organic carbon and nitrogen and phosphorus resources as carbon sink and nutrient sources is neglected in the prior art.
Absstract of: WO2026126194A1
This invention relates to a hybrid photobioreactor device capable of facilitating the occurrence of light-dark reactions in a sequential process within a single system. This is done to increase biomass production of microalgae/cyanobacteria cultures, with real-time monitoring of growth parameters. This device is functionally divided into five components: 1) a closed flat-panel bioreactor unit; 2) a light source platform unit; 3) a microalgae culture suspension transfer pump unit; 4) a dark-tube bioreactor unit made of opaque material where the dark reactions occur; and 5) a data logger module to monitor photosynthesis parameters. The ratio of the microalgae culture residence time in the light-panel bioreactor, where the light reactions occur, to the residence time in the dark-tube bioreactor, where the dark reactions occur, is adjusted to achieve effective and efficient operating conditions to support microalgae growth.
Absstract of: WO2026127648A1
The present invention relates to a method for producing polyhydroxyalkanoate using a microalgal biomass hydrolysate. According to the present invention, PHA productivity can be improved by using a microalgal biomass hydrolysate for PHA production, and the chain length of PHA can be controlled according to the accumulation levels of lipids and carbohydrates in microalgae. Accordingly, the present invention provides the advantage of enabling the customized production of PHA having physical properties suitable for various applications. In addition, the present invention can replace existing biomass and can be effectively applied to PHA production processes requiring cost-effectiveness and eco-friendliness.
Absstract of: WO2026127757A1
A method for cultivating microalgae comprises the steps of inoculating a culture media with microalgae and adding a substrate into the inoculated culture media; and cultivating the microalgae under heterotrophic growth conditions and periodically introducing microscopic air bubbles and the substrate into the inoculated culture media.
Absstract of: WO2026125642A1
The present invention relates to a biotechnological process for the production of a OMWW fermented extract comprising the following steps: a) providing and mixing OMWW and microalgae Chlorella, thus obtaining a starting material; b) inoculating the starting material with a lactic acid bacterium and/or a yeast, and c) fermenting the starting material. The fermented OMWW extract obtained has the nutritional and functional profiles that enrich the nutritional characteristics of the product contained it, for example wheat bread, introducing notable biological and antioxidant compounds. Simultaneously, the products of the invention exhibited enhanced antifungal properties and extended shelf life especially.
Absstract of: WO2025032068A1
The present invention relates to a process for producing propylene and to a cyanobacterial cell for the production of propylene.
Absstract of: GB2702486A
The present invention relates to the use of carbon nanodots to treat wastewater. Specifically, the invention relates to the use of carbon nanodots for improving the efficiency of microorganism-mediated wastewater treatment, in particular carbon nanodots improve wastewater treatment involving bacteria, such as activated sludge treatment, treatment with photosynthetic bacteria, and also in treatments using microalgae. The use of carbon nanodots to control flocculation and/or sedimentation times in microorganism-mediated wastewater treatment is also described. A wastewater treatment solution comprising: wastewater, microorganisms suitable for wastewater treatment, and carbon nanodots; and a method for treating wastewater comprising the step of preparing the solution, are described. The carbon nanodots can contain surface polyethylene glycol functionality and/or surface polyethyleneimine functionality. The wastewater can comprise organic and/or inorganic matter. Figure 1a
Absstract of: KR20260091480A
0001a 본 발명은 미세조류 바이오매스 당화액을 이용한 폴리하이드록시알카노에이트 제조방법에 관한 것이다. 본 발명에 따르면, 미세조류 바이오매스 당화액을 PHA 생산에 이용함으로써 PHA의 생산성을 향상시킬 수 있고, 미세조류의 지질 및 탄수화물 축적량에 따라 PHA의 사슬 길이를 조절할 수 있다. 이에 따라 본 발명은 다양한 용도에 적합한 물성을 갖는 PHA를 맞춤형으로 제조할 수 있는 이점을 제공한다. 또한 본 발명은 기존의 바이오매스를 대체할 수 있으며, 경제성과 친환경성이 요구되는 PHA 생산 공정에 유용하게 적용될 수 있다.
Absstract of: CN122212371A
The invention discloses an application of Platymonas subcordiformis in removal of phosphorus nutritive salts in mariculture wastewater, the Platymonas subcordiformis is Platymonas sp.CCMA-317, is preserved in the China Center for Type Culture Collection on January 20, 2026, and has a preservation number of CCTCC NO: M2026173, and the preservation number of the Platymonas subcordiformis is CCTCC NO: M2026173. The application adopts any one of the following culture modes: carrying out semi-continuous culture by taking the pretreated industrial mariculture wastewater as a substrate; or carrying out batch culture by taking open in-situ mariculture water as a substrate; or constructing a phycomycete symbiotic system with a halomarinobacter strain for culturing. The whole process does not need to domesticate algae species in advance, the pretreatment steps are simple (only conventional bolting-silk filtration, gravity settling, commercially available disinfectants and antichlor are adopted), the culture conditions are mild and easy to control, the technical threshold and the operation cost are remarkably reduced, and the method is suitable for industrial production. The defects of secondary pollution of a traditional chemical method, difficulty in domestication of a biological method and low batch culture efficiency of the existing microalgae are overcome, and the method conforms to green sustainable development and circular economy concepts and has a good industrial popularization
Absstract of: CN122214462A
The invention belongs to the technical field of algae strain breeding. The invention discloses a breeding method of a high-yield exopolysaccharide nannochloropsis algal strain. According to the breeding method, a nannochloropsis algal strain is taken as an object, an algal solution in a logarithmic phase is subjected to ARTP mutagenesis treatment, the mutagenesis fatality rate is controlled to be not lower than 90%, a mixed solution of glycerol and a BG-11 culture medium is taken as a protective solution to resuspend the algal body, and screening is performed after dark culture. By taking the standard that the yield of the extracellular polysaccharide is not lower than 20% before mutagenesis, the extracellular polysaccharide nannochloropsis algal strain with high yield and genetic stability is obtained by combining two-step screening of plate primary screening and fermentation secondary screening. According to the method, ARTP mutagenesis and directional screening are combined, the obtaining rate of high-yield extracellular polysaccharide algal strains can be effectively increased, the bred algal strains are stable in character, and the method is suitable for fermentation production of microalgae extracellular polysaccharide.
Absstract of: CN122214234A
The invention belongs to the technical field of water pollution treatment, and particularly relates to a three-phase phycomycete symbiont based on plant hormone combination and a construction method and application thereof. The three-phase phycomycete symbiont disclosed by the invention consists of dominant microalgae (chlorella), functional bacteria (S395-2) and functional fungi (helicosporium), and is obtained through combined regulation and control of plant hormones GA3 and GR24; the constructed three-phase phycomycete symbiont is high in balling stability and strong in heavy metal and residual antibiotic stress resistance, the content of extracellular polymeric substances (EPS) can be remarkably increased, the adsorption fixation and reduction effect on multiple ARGs such as sulfonamides, tetracyclines and macrolides in swine wastewater can be enhanced, the total reduction rate of the ARGs can reach 90% or above, and the total reduction rate of the ARGs can reach 90% or above. The symbiont can be subsequently developed into feed or food, the risk of secondary pollution is avoided, good practicability and economical efficiency are achieved, and an efficient and stable technical scheme is provided for pollution treatment of the pig raising wastewater ARGs.
Nº publicación: CN122214117A 16/06/2026
Applicant:
WENZHOU JIYIN TECH CO LTD
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Absstract of: CN122214117A
The invention discloses a microalgae three-dimensional culture device, and relates to the technical field of microalgae culture, the microalgae three-dimensional culture device comprises a collection tank and a substrate, the top of the collection tank is provided with a detachable cover, one side of the bottom of the collection tank is provided with a discharge pipe, one side of the top of the collection tank is connected with a water outlet of a water pump through a drain pipe, and a water inlet of the water pump is connected with a water inlet pipe; a water inlet of the water inlet pipe is detachably connected with the transparent culture barrel; a plurality of combined supports are detachably installed on the top of the base plate, a plurality of transverse frames are installed between the combined supports, and the combined supports and the transverse frames are jointly provided with a plurality of vertically-distributed transparent culture barrels; communicating openings are formed in the upper side and the lower side of the two ends of the transparent culture barrel, and sealing plugs are installed at the communicating openings in a threaded mode; according to the device, convenient vertical combination can be achieved according to needs, so that three-dimensional culture of algae is achieved, the device irradiates the algae through natural light and a light supplementing lamp at the same time, it can be guaranteed that the algae fully absorb and utilize illumination a