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METHODS AND COMPOSITIONS FOR DETECTING VIRULENT AND AVIRULENT ESCHERICHIA COLI STRAINS

Resumen de: US20260071283A1

Disclosed herein are methods for detecting virulent Shiga toxin-producing E. coli (STEC) strains O26, O103, O121, and O111 in a biological sample comprising the steps of: (i) enriching the bacterial concentration of the biological sample to result in an enriched sample; (ii) isolating DNA from said enriched biological sample; and (iii) detecting virulent strain in said isolated DNA sample via real-time PCR and a melt curve assay. Also disclosed are primers for said assay, as well as kits comprising said primers.

Primer set for detecting Listeria monocytogenes or Listeria innocua a kit comprising the same and a method for detecting the strain

Resumen de: KR20260032656A

본 발명은, 리스테리아 모노사이토제네스(Listeria monocytogenes) 또는 리스테리아 이노쿠아(Listeriainnocua)의 검출용 프라이머 세트, 이를 포함하는 키트 및 상기 균의 검출 방법에 대한 것이다.

Lateral flow strip based on loop-mediated isothermal amplification for detection of Salmonella

Resumen de: KR20260030992A

본 발명은 살모넬라 검출을 위한 고리매개등온증폭법 기반 측방유동스트립에 관한 것으로, 보다 상세하게는 고리매개등온증폭(loop-mediated isothermal amplification)용 프라이머 세트; 상기 프라이머 세트에 의해 증폭되는 증폭산물에 특이적으로 결합하는 프로브; 및 측방유동스트립(lateral flow strip);을 포함하는, 살모넬라 속 균을 검출하기 위한 측방유동검사(lateral flow assay) 키트 및 상기 키트를 이용한 살모넬라 속 균의 검출방법에 관한 것이다.

DIGITAL PCR ASSAY FOR THE DETECTION OF ENTEROHEMORRHAGIC ESCHERICHIA COLI

Resumen de: US20260062763A1

The present disclosure relates primer pairs, probes, kits, and methods of use thereof to detect virulent strains of Shiga toxin-producing Escherichia coli (E. coli) (STEC).

BIOSENSOR, METHOD FOR OBTAINING THE BIOSENSOR, METHOD FOR DETECTING PSEUDOMONAS AERUGINOSA AND DETECTION KIT USING THE SENSOR FOR THE RAPID DETECTION OF P. AERUGINOSA

Resumen de: WO2026047265A1

The present invention relates to a biosensor, a method for obtaining same and the use thereof to detect Pseudomonas aeruginosa in clinical samples from patients, based on the use of molecular gates on porous organic-inorganic hybrid supports that allow specific and selective recognition of bacterial DNA. The molecular gate developed comprises an oligonucleotide sequence complementary to a specific region of the DNA of this bacterium. The external surface of the porous support is chemically modified to provide reactive groups capable of bonding with short-sequence oligonucleotides (O1) designed to act as an anchor and to subsequently bond oligonucleotides (O2) specific for the recognition of P. aeruginosa. In the presence of P. aeruginosa genomic DNA, the oligonucleotides (O2) are capable of specific recognition by complementarity and of detaching from the pore surface, leading to the release of a fluorescent indicator.

Salmonella viable bacteria detection system based on deoxyribozyme activation and method thereof

Resumen de: CN121575133A

The invention belongs to the technical field of biological analysis and detection, and particularly discloses a live salmonella detection system and method based on deoxyribozyme activation, the detection system comprises a Sub probe, a deoxyribozyme probe, a hairpin chain probe, a hyperbranched dendritic nano-molecule, a crRNA/Cas12a binary compound, a fluorescent probe and magnetic beads, the hyperbranched dendritic nano-molecule comprises a substrate A, a substrate B and a trigger chain B probe, the substrate A is composed of an A-F chain probe, an A-Q chain probe and an auxiliary chain A probe, and the substrate B is composed of a B-F chain probe, a B-Q chain probe and an auxiliary chain B probe. According to the detection system, ribonuclease H2 released only by metabolism of living salmonella is specifically recognized, and is utilized to activate a Sub-Dz substrate, so that the subsequent reaction can be triggered only by viable bacteria from the source, the problem of dead bacteria interference is effectively solved, and high-sensitivity and high-specificity rapid detection of the viable salmonella is realized.

LAMP primer group, kit and method for detecting pathogens in fresh milk

Resumen de: CN121575130A

The invention belongs to the technical field of biology, and particularly relates to an LAMP primer group, a kit and a method for detecting pathogens in fresh milk, a visual LAMP detection method of listeria monocytogenes is established by taking a gyrB gene of the listeria monocytogenes as a target, and a visual LAMP detection method is established by taking a mycobacterium tuberculosis Rv2875 gene as a target. The kit has good specificity and good sensitivity, and still has good specificity and sensitivity when being used for detecting artificially polluted cow milk.

Hot spot self-assembly colorimetric-Raman sensing platform and escherichia coli detection method

Resumen de: CN121575084A

The invention discloses a hot spot self-assembly colorimetric-Raman sensing platform and an escherichia coli detection method, the hot spot self-assembly colorimetric-Raman sensing platform comprises an isothermal amplification system, a CRISPR-dCas9 system, GNPs-probe and SA-GNPs, a target bacterial gene is taken as a DNA template, RPA amplification is carried out through the isothermal amplification system, and a double-chain amplification product modified by terminal biotin is obtained; the CRISPR-dCas9 system is used for targeted recognition of a double-chain amplification product modified by terminal biotin to form a composite product; gNPs-probe is composed of gold nanoparticles, a Raman signal molecule and a DNA probe, and the GNPs-probe is combined with the stem-loop structure of the sgRNA of the composite product through the DNA probe; sA-GNPs is combined with the biotin of the composite product through streptavidin; gNPs-probe and SA-GNPs are assembled on the composite product, so that a colorimetric-Raman sensing platform for detecting target bacteria is formed. According to the present invention, the cross validation of the generated colorimetric, ultraviolet and Raman multi-mode signals is adopted to improve the result reliability, the isothermal amplification and the self-assembly hot spot are adopted to enhance the multiple sensibilization detection signal, and the specificity is enhanced based on the specific primer group and the CRISPR-dCas9 system mediated s

Intelligent auxiliary system for single-tube detection of food-borne pathogenic bacteria

Resumen de: CN121574809A

According to the intelligent auxiliary system for single-tube detection of the food-borne pathogenic bacteria, the One-pot-RPA-CRISPR/Cas12a reaction system is combined with the embedded artificial intelligence detection device, so that high-sensitivity and high-specificity detection and automatic grading judgment of the food-borne pathogenic bacteria are realized; through wireless interaction between the portable detection equipment and the intelligent terminal, the portability and field applicability of the equipment are realized; by introducing a YOLOv8n model and an OpenCV image processing algorithm, automatic identification and quantitative analysis of fluorescence signals are realized, subjective errors of manual interpretation are avoided, and the detection efficiency and consistency are improved; the system has good expandability, can meet the detection requirements of various pathogens or biomarkers by replacing primers and crRNA, is suitable for various scenes such as food pollution monitoring, primary medical diagnosis, family health management and the like, and has wide social benefits and application prospects.

Listeria monocytogenes detection kit and detection method based on iron-tannic acid network coating aggregation-induced emission nanodots

Resumen de: CN121555660A

The invention provides a listeria monocytogenes detection kit based on iron-tannic acid network coating aggregation-induced emission nanodots and a detection method. The listeria monocytogenes detection kit comprises Cas12a protein, crRNA, a single-stranded nucleic acid fluorescent probe, an iron-tannic acid network coating aggregation-induced emission nanodot dispersion liquid, a Lamp element, a buffer solution and enzyme-free water. According to the present invention, the iron-tannic acid network coating aggregation-induced emission nano-dot is adopted as the light-emitting material, Cy5-ssDNA is adopted as the fluorescent probe, and the specific gene segment Listeria of Listeria monocytogenes is adopted as the target sequence to construct the two-color ratio fluorescent biosensor, and the two-color ratio fluorescent biosensor can be used for detection of Listeria monocytogenes in food, the method has the advantages of simplicity, no dependence on large instruments, low cost, short detection period, low background, high sensitivity, strong specificity, high efficiency, reliability and the like.

HanddRPA detection method for litchi pathogenic bacteria and application of handdRPA detection method

Resumen de: CN121555674A

The invention relates to a handdRPA (recombinase polymerase amplification) detection method for litchi pathogenic bacteria and application, the handdRPA detection method has high specificity for litchi peronophythora, only aims at specific sequence amplification of a target pathogen P. litchii, and does not generate cross reaction with common plant pathogenic bacteria and environmental microorganisms. According to the detection method, on the premise of no cross amplification, the accuracy and reliability of detection are guaranteed, and false positive is effectively avoided; and moreover, high-specificity and accurate detection consistent with P.litchii can be realized under a complex microorganism background, and the requirement of rapid field detection in a complex sample is met. In practical application, the specificity provides a solid foundation for field pathogen detection, and also provides technical support for popularization and application of handdRPA technology in pathogen field detection.

CRISPR/Cas12a-based double-layer microfluidic electrochemical biosensor as well as preparation method and application thereof

Resumen de: CN121540789A

The invention belongs to the technical field of biosensing analysis and food safety detection, and particularly relates to a CRISPR/Cas12a-based double-layer microfluidic electrochemical biosensor as well as a preparation method and application thereof. A single-walled carbon nanohorn-polypyrrole-gold nanoparticle compound modified electrode is constructed and integrated with an electrochemical detection system, then the electrochemical detection system and a micro-fluidic chip are integrated to obtain the double-layer micro-fluidic electrochemical biosensor, targeted detection of different pathogenic bacteria can be achieved by replacing crRNA, and the electrochemical biosensor has the advantages of high specificity, high sensitivity, high sensitivity and the like. Good universality and expansibility are realized; the biosensor can rapidly and sensitively detect staphylococcus aureus and salmonella in dairy products, the detection ranges are 1.06 * 10 < 1 >-1.06 * 10 < 7 > CFU/mL and 1.04 * 10 < 1 >-1.04 * 10 < 7 > CFU/mL respectively, the detection limits are as low as 3 CFU/mL, and the biosensor has good practicability.

Method for detecting salmonella in pasteurized milk

Resumen de: CN121522155A

The invention discloses a method for detecting salmonella in pasteurized milk, relates to the technical field of food detection, and provides a one-step rapid high-sensitivity detection method suitable for salmonella in pasteurized milk by constructing a platinum-based polymer nano-enzyme composite probe and combining immunomagnetic bead separation and signal amplification strategies. Based on the excellent peroxidase-like activity of the platinum-based polymer nano-enzyme, the composite probe can catalyze a chromogenic substrate to generate a strong signal amplification effect in the presence of low-concentration target bacteria, so that the detection limit of salmonella in a pasteurized milk matrix as low as 10 CFU/mL is realized; compared with a traditional culture method and part of existing immunological methods, the sensitivity is improved by one to two orders of magnitude, and the detection capacity of low-pollution-level salmonella is remarkably enhanced.

PfAgo detection system, RPA primer group, kit and method for synchronously detecting staphylococcus aureus and escherichia coli

Resumen de: CN121518674A

The invention provides a PfAgo detection system for synchronously detecting staphylococcus aureus and escherichia coli, an RPA primer group, a kit and a method, and belongs to the technical field of pathogenic bacterium detection. The system comprises PfAgo protein, at least two fluorescent report probes respectively corresponding to staphylococcus aureus and escherichia coli, and at least two groups of specific guide DNA (deoxyribonucleic acid), namely guide gDNA. According to the invention, RPA isothermal amplification and PfAgo protein-mediated nucleic acid cleavage reaction are combined, so that high-specificity and high-sensitivity rapid detection of two pathogenic bacteria is realized. The method is short in detection time, easy and convenient to operate, free of complex instruments and suitable for on-site rapid screening and multiple detection of pathogenic bacteria in complex matrixes such as food and cosmetics.

Method for separating, enriching and rapidly detecting listeria monocytogenes

Resumen de: CN121499800A

The invention belongs to the technical field of gene detection, and provides a method for separating, enriching and rapidly detecting Listeria monocytogenes, which comprises the following steps: S1, preparing immunomagnetic beads labeled with an anti-Listeria monocytogenes monoclonal antibody 2F9; s2, mixing the immunomagnetic beads obtained in the step S1 with a sample to be detected, and enriching listeria monocytogenes through a magnetic separation technology; s3, preparing a rapid detection kit; s4, detecting the enriched listeria monocytogenes by using the rapid detection kit in the step S3; by preparing the immunomagnetic beads labeled with the listeria monocytogenes monoclonal antibody 2F9, specific separation and enrichment of listeria monocytogenes in a sample are realized, and the problems of high target strain acquisition difficulty and long culture period in enrichment of a traditional culture method are solved; meanwhile, the defect that an existing gene detection method cannot specifically separate and enrich target bacteria is overcome, and the detection pertinence and efficiency are greatly improved.

Cell sensor based on four-channel electrode device and application of cell sensor in pathogenic bacterium detection

Resumen de: CN121499631A

The invention discloses a cell sensor based on a four-channel electrode device and application of the cell sensor in pathogenic bacterium detection, and belongs to the technical field of analysis and detection. The cell sensor is formed by combining HEK 293/hNAIP-NLRC4 cells serving as a recognition element and a ready-to-use cell culture paper chip serving as a carrier with a four-channel electrode device with a cell culture pool. The sensor constructed by taking HEK 293/hNAIP-NLRC4 cells as a recognition element is high in sensitivity, can distinguish escherichia coli pathogenic bacteria with T3SS from escherichia coli non-pathogenic bacteria without T3SS, specifically perceives various pathogenic bacteria with T3SS, specifically recognizes non-targeted food-borne pathogenic bacteria, and provides a new thought for risk detection and research of unknown bacteria in food.

Method for detecting salmonella typhimurium based on strand displacement reaction and CRISPR/Cas12a in combination with colorimetric sensor and dairy product detection method

Resumen de: CN121496077A

The invention discloses a method for detecting salmonella typhimurium based on strand displacement reaction and CRISPR/Cas12a in combination with a colorimetric sensor and a dairy product detection method, and belongs to the technical field of food safety rapid detection. The invention provides a method for detecting salmonella typhimurium based on strand displacement reaction and CRISPR (clustered regularly interspaced short palindromic repeats)/Cas12a combined with a colorimetric sensor in order to solve the problems of low sensitivity and inaccurate result of detection of salmonella typhimurium in dairy products by a traditional method in the prior art. According to the method, DNA-Ag/Pt NCs is used as a signal molecule to construct the aptamer colorimetric sensor for detecting salmonella typhimurium; the colorimetric sensor has relatively high sensitivity and specificity, and can quickly and conveniently realize on-site detection of salmonella typhimurium.

Detection equipment for detecting escherichia coli

Resumen de: CN121495674A

The detection equipment comprises a detector shell and a sampling tube, the sampling tube is of an up-down split structure, the upper portion and the lower portion of the sampling tube are connected in an inserted mode, a fixing frame is arranged on one side of the detector shell, and a fixing rod and a movable rod are arranged in the fixing frame. When the device is implemented, the upper part and the lower part of the sampling tube can be separated by driving the connecting block, and after the upper part of the sampling tube moves to a preset position, the upper part of the sampling tube does not move upwards along with the continuous upward movement of the connecting block, and at the moment, a sample is placed on the sample loading frame and pushed into the fixed frame, so that the sampling tube can be fixed. The cotton swab in the sampling tube can be in full contact with the surface of a sample to achieve the sampling purpose, then the sampling tube is placed in the detector, escherichia coli in the sample can be detected, the sampling process can be more standardized through the arrangement of the device, and errors caused by manual sampling are avoided.

Specific primer for detecting swine edema disease and application thereof

Resumen de: CN121472438A

The invention discloses a specific primer pair for detecting a swine edema disease. The specific primer pair comprises a forward primer Stx2eF and a reverse primer Stx2eR, wherein the nucleotide sequence of the forward primer Stx2eF is as shown in SEQ ID NO: 1, and the nucleotide sequence of the reverse primer Stx2eR is as shown in SEQ ID NO: 2. Data show that the detection result of a sample inoculated with Shiga toxin-producing escherichia coli containing the Stx2e gene by using the primer pair disclosed by the invention is highly consistent with the actual infection degree, and the pathogenic bacterium load can be dynamically quantified through a standard curve. By means of the advantage, early warning of the pig edema disease can be achieved, and a key time window is provided for early prevention and control.

Bimodal biosensor detection kit for detecting escherichia coli and preparation method of bimodal biosensor detection kit

Resumen de: CN121476591A

The invention particularly relates to a bimodal biosensor detection kit for detecting escherichia coli and a preparation method, and belongs to the technical field of food safety detection. The bimodal biosensor detection kit is composed of reticular targeted magnetic beads and a biosensor detection reagent. A biosensor detection reagent is a copper metal doped carbon dot solution modified by an aptamer, and the aptamer is an aminated aptamer. The dual signals provided by the dual-mode sensor can be mutually calibrated, accidental errors of single signal output are avoided, and advantage detection modes can be selected according to different scenes; a reticular PGA structure is used as an intermediate to coat ferroferric oxide magnetic beads, and a high-load reticular structure provides more binding sites for a recognition element, so that the capture efficiency of a magnetic separation technology is further enhanced; the bimetallic targeted modified carbon dots driven by the enhanced magnetic separation technology have good detection performance when being used for detecting escherichia coli O157: H7, and the efficiency of capturing low-concentration target bacteria in a food matrix can reach 90% or above.

Folic acid-o-phenylenediamine co-doped carbon quantum dot, Escherichia coli O157: H7 detection probe, detection reagent and application

Resumen de: CN121471909A

The invention belongs to the technical field of food safety detection, and particularly relates to folic acid-o-phenylenediamine co-doped carbon quantum dots, an Escherichia coli O157: H7 detection probe, a detection reagent and application. The folic acid-o-phenylenediamine co-doped carbon quantum dots are prepared by heating folic acid and o-phenylenediamine to react. When the detection probe or the detection reagent based on the folic acid-o-phenylenediamine co-doped carbon quantum dots is used for detecting Escherichia coli O157: H7, the detection limit is low, the specificity is excellent, and the practicability is high.

Monoclonal antibody combination for Brucella detection and application thereof

Resumen de: CN121449738A

The invention discloses a monoclonal antibody combination for Brucella detection and application thereof, and belongs to the technical field of immunodetection. The monoclonal antibody combination disclosed by the invention comprises a monoclonal antibody 15F4 and a monoclonal antibody 32H9. On the basis of the monoclonal antibody combination, a double-antibody sandwich ELISA detection method with 15F4 as a capture antibody and enzyme-labeled 32H9 as a detection antibody is established, and key parameters such as coating concentration, closing conditions, incubation and color development time and the like are optimized. The method has high specificity on brucella, and does not cross with common cross-reactive bacteria such as escherichia coli O: 157, salmonella and the like; the sensitivity is high, and the detection limit can reach 1 * 10 < 5 > CFU/mL; the repeatability is good, and the intra-batch and inter-batch variation coefficients are both smaller than 10%. The invention provides a core reagent and a reliable means for early, rapid and accurate diagnosis of brucellosis, and is suitable for on-site screening of clinical samples and animal epidemic diseases.

Pretreatment equipment for escherichia coli detection

Resumen de: CN121450414A

The invention relates to the technical field of microorganism detection automation equipment, and discloses pretreatment equipment for escherichia coli detection, which solves the problems in the background technology, and comprises a machine base, and a filling station and a rotary driving assembly arranged on the machine base. The driving assembly drives the L-shaped workbench to rotate between the initial station and the processing station through the sleeve. A bag opening clamping mechanism is arranged on the side of the sleeve, the bag opening is fixed through an elastic self-adaption module, and the pulling force generated when the bag opening is closed can be effectively buffered. A bag body processing mechanism is arranged on the horizontal portion of the L-shaped workbench and comprises a sealing clamp driven by a two-way lead screw and provided with a sealing convex rib and a groove, and an inertia flapping assembly driven by an air cylinder and capable of conducting reciprocating motion. The flapping assembly achieves soft flapping through the flexible flapping plate by means of inertia. According to the device, the bag lifting and flapping actions of human hands are simulated through the automatic mechanism, automatic filling, sealing and efficient flapping mixing of homogeneous bags are achieved, and the automation degree and the treatment efficiency of pretreatment are improved.

Multi-drug resistance gene detection system and method based on suspension array technology and application

Resumen de: CN121428129A

The invention discloses a multi-drug-resistance gene detection system and method based on a suspension array technology and application. The multi-drug-resistance gene detection system comprises coding microspheres, a primer group, a fluorescence labeled anti-Tag ssDNA molecule and phycoerythrin. The invention constructs a detection method aiming at a plurality of bacteria and drug-resistant genes by a suspension array technology, designs a plurality of pairs of specific primers of bacteria and drug-resistant genes with Tag labels, captures Tag labeled products after multiple PCR amplification by anti-Tag fixed on a suspension array, realizes accurate detection, and has the advantages of high specificity, high sensitivity, high sensitivity and high sensitivity. According to the present invention, with the kit, the four types of bacteria (E. coli, A. brumanni, K. pneumonae and P. aeruginosa) and the eight types of drug-resistant genes (blaKPC-2-like, blaNDM-like, blaOXA-48-like, blaOXA-23-like, blaIMP-like, blaVIM-like, blaCMY-2-like and blaADC-68-like) can be simultaneously detected in the same reaction system, such that the reagent consumption is reduced, the cost is reduced, and the detection time is shortened.

Biosensor for detecting pseudomonas aeruginosa and preparation method thereof

Nº publicación: CN121431842A 30/01/2026

Solicitante:

CHINA ACAD OF QUALITY INSPECTION & TESTING SCIENCES
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Resumen de: CN121431842A

The invention discloses a multivalent aptamer fluorescent biosensor for detecting pseudomonas aeruginosa and a preparation method of the multivalent aptamer fluorescent biosensor, sequence template design is carried out based on an aptamer (F23), a long multivalent ligand with repeated aptamer units is synthesized through RCA rolling circle amplification, and through the synergistic effect between the aptamer units and a formed'capture net ', the fluorescent biosensor is used for detecting the pseudomonas aeruginosa. The binding efficiency with the target pseudomonas aeruginosa is remarkably improved, and the sensitivity of a detection system is enhanced.