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Kit containing pushing mechanism and used for detecting plant pathogenic bacteria and detection method of kit

Resumen de: CN121425649A

The invention relates to the technical field of plant pathogenic bacterium detection, in particular to a plant pathogenic bacterium detection kit containing a pushing mechanism and a detection method thereof.The kit comprises a lower base and an upper base, the upper base and the lower base are movably installed together through a hasp, and a receding groove is formed in the upper end of the lower base; a groove is formed in one side of the receding groove, a rotating disc is movably connected into the receding groove, a connecting cavity is formed in the lower end of the upper base, and a plurality of sets of containing holes are formed in the upper end of the rotating disc and used for containing reagent bottles. The motor drives the rotating disc to rotate, so that the multiple groups of reagent bottles are driven to move, when the reagent bottles needing to be used move to the position of the glass window, the motor is turned off, the guide wheel located at the position of the glass window enters the groove at the moment, and dust, insects and other external substances are prevented from entering the cavity; the condition that the reagent is damaged or deteriorated is avoided.

Method for detecting and identifying shiga toxin producing escherichia coli and/or enterohemorrhagic escherichia coli

Resumen de: CN121443757A

The present invention relates to a method for detecting and identifying at least one Shiga toxin-producing Escherichia coli (STEC) that may be present in a sample containing enterobacteria, said method comprising the following steps:-lysing the sample in order to lyse the STEC, thereby obtaining a solution comprising the nucleic acids thereof; -contacting said nucleic acid solution with primers to allow amplification of at least the stx1 and/or stx2 genes or gene fragments; -if amplified to at least one of the stx1 and/or stx2 genes or gene fragments, placing a portion of the sample onto an agar reaction medium comprising: at least one toxin inducer, at least one agglutination conjugate, at least one agglutination conjugate, at least one poison inducer, at least one poison inducer, at least one poison inducer, at least one poison inducer, at least one poison inducer, and at least one poison inducer. The binding partner is formed by at least one binding partner specific to the STX1 protein and/or at least one binding partner specific to the STX2 protein, and the binding partner is coupled with the nanoparticle; -detecting and confirming the presence of at least one STEC by the presence of a halo on the agar around said STEC.

Nucleic acid combination product, kit and method for detecting various respiratory tract pathogenic bacteria

Resumen de: CN121406811A

The invention discloses a nucleic acid combination product, a kit and a method for detecting various respiratory tract pathogenic bacteria. The nucleic acid combination product comprises a primer pair and a probe, wherein the sequences of the primer pair and the probe are respectively shown as SEQ ID NO: 1-SEQ ID NO: 24. The nucleic acid combination product realizes synchronous detection of various pathogens through a one-tube eight-color fluorescent PCR, freeze-drying and ultrasonic direct diffusion method technology, significantly reduces experimental operation steps, shortens detection time, reduces the complexity of result interpretation, and has strong anti-interference capability and high sensitivity.

Dual MIRA detection method for type A clostridium botulinum and staphylococcus aureus for portable reagent card incubator and application of dual MIRA detection method

Resumen de: CN121406806A

The invention belongs to the technical field of biological detection, and particularly relates to a dual MIRA detection method for type A clostridium botulinum and staphylococcus aureus for a portable reagent card incubator. The double colloidal gold type MIRA method established by the invention has good specificity on type A clostridium botulinum and staphylococcus aureus, and has no cross reaction with six strains such as pasteurella multocida, klebsiella pneumoniae and salmonella. The lowest detection limits of the method on the staphylococcus aureus and the A-type clostridium botulinum are 2.94 * 100 copies/mu L and 2.27 * 100 copies/mu L respectively. The optimized double colloidal gold type MIRA method can be successfully applied to a reagent card incubator. The detection method disclosed by the invention has the advantages of good specificity, high sensitivity, simplicity in operation, short time consumption and the like.

CRISPR (clustered regularly interspaced short palindromic repeats)-based food-borne pathogenic bacterium crRNA (ribonucleic acid), kit, multiple detection method and application

Resumen de: CN121380063A

The invention discloses CRISPR (clustered regularly interspaced short palindromic repeats)-based food-borne pathogenic bacterium crRNA (crRNA), a kit, a multiple detection method and application, and belongs to the technical field of food safety detection. Specific genes of salmonella typhimurium (invA), staphylococcus aureus (femB), listeria monocytogenes (hly) and vibrio parahaemolyticus (toxR) are used as target genes for detection, an RPA primer and crRNA are designed for each gene, a quadruple RPA-CRISPR reaction system is established, the dual technical advantages of RPA amplification and a CRISPR system are combined, the detection sensitivity of the method is smaller than 10 copies/L, the detection time is greatly shortened, and the detection efficiency is greatly improved. And visual detection is realized through a blue light detector.

Escherichia coli colorimetric detection method established based on aptamer enhanced octahedral Ag2O nanoparticle simulation oxidase activity

Resumen de: CN121380277A

The invention discloses an Escherichia coli colorimetric detection method established based on aptamer enhanced octahedral Ag2O nanoparticle simulation oxidase activity, which is characterized by comprising the following steps: (1) fully and uniformly mixing dispersion liquid of octahedral Ag2O NPs and P12-55 aptamer solution, and then incubating; (2) adding a test sample into the mixed solution, and continuing incubation; and (3) adding TMB and a NaAc buffer solution, carrying out ultraviolet/visible spectrum analysis at room temperature, recording absorbance values of the test sample and the blank sample at 652nm, and calculating the change of the absorbance values to obtain a detection result.

High-flux real-time fluorescent quantitative PCR (polymerase chain reaction) chip for detecting soil transmission pathogenic bacteria and detection method thereof

Resumen de: CN121380410A

The invention discloses a high-flux real-time fluorescent quantitative PCR (polymerase chain reaction) chip for detecting soil-transmitted pathogenic bacteria and a detection method of the high-flux real-time fluorescent quantitative PCR chip. The chip comprises a primer composition consisting of 64 pairs of primer pairs which are designed for specific genes of 47 species and 7 genera of soil-propagated plant pathogenic fungi, 8 species of soil-propagated plant pathogenic bacteria and two reference genes. On the basis of a high-flux quantitative PCR (HT-qPCR) technology, a novel chip capable of simultaneously detecting 47 species and 7 genera of soil-transmitted plant pathogenic fungi and 8 species of soil-transmitted plant pathogenic bacteria is established, and a powerful tool is provided for evaluating potential risks of soil-transmitted plant pathogens in an environment. The method is applied to pathogenic bacteria spread in soil, the abundance and distribution of the pathogenic bacteria spread in the soil can be detected and analyzed, the defects of low-flux amplification of traditional single-gene qPCR and high cost of metagenomics are overcome, and the method has wide application prospects.

Reaction culture medium and method for detecting shiga toxin-producing escherichia coli and/or enterohemorrhagic escherichia coli

Resumen de: CN121399274A

The present invention relates to a gelling reaction medium for the detection, identification and/or isolation of at least one Shiga toxin-producing Escherichia coli strain, the reaction medium comprising:-at least one toxin-inducing agent,-at least one agglutination conjugate, -a nanoparticle comprising at least one specific binding partner of STX1 and/or at least one specific binding partner of STX2 conjugated to the nanoparticle,-a concentration gradient of a compound for inhibiting non-target bacteria. The invention also relates to related methods of detecting and/or isolating Shiga toxin-producing Escherichia coli that may be present in a sample containing intestinal bacteria.

Salmonella typhimurium fluorescence detection method based on double signal amplification

Resumen de: CN121380294A

The invention discloses a salmonella typhimurium fluorescence detection method based on dual signal amplification, and relates to the technical field of microbiological detection.The method comprises the specific steps that firstly, SNA1 and SNA2 probes are constructed, related chains are co-modified on the surfaces of gold nanoparticles through a freezing method, and part of sequences are closed; then preparing an Apt.-F compound; then incubating the sample and the compound; then starting a dual signal amplification reaction; and finally, detecting the fluorescence intensity by using a fluorospectro photometer, and quantifying the salmonella typhimurium according to the relationship between the fluorescence intensity and the bacterium concentration. According to the invention, two kinds of functionalized SNA probes are constructed, the aptamer and DNAzyme are arranged, a dual signal amplification pathway is established, target bacteria are accurately identified through the aptamer, a trigger chain is released to start chain replacement, a fluorophore is released, the detection sensitivity is improved, the probe design is optimized to ensure specificity, the process is simplified, and rapid quantification is realized; an efficient scheme is provided for trace pathogenic bacterium detection.

High-sensitivity aquatic pathogenic bacteria nucleic acid detection platform and method based on DNAzyme collaborative mechanism

Resumen de: CN121344161A

The invention belongs to the technical field of gene detection, and particularly relates to a high-sensitivity aquatic pathogenic bacterium nucleic acid detection platform and method based on a DNAzyme collaborative mechanism. According to the invention, through the synergistic effect of hD or multiple DNAzyme, the secondary structure of the target ssDNA is effectively and competitively dissociated, and the steric hindrance of the secondary structure on the combination of DNAzyme is eliminated, so that the detection sensitivity reaches an extremely low level of 1 CFU/mL; according to the invention, a chain intrusion coordination mechanism (hD or adjacent multiple DNAzyme) is systematically introduced in DNAzyme detection for the first time, the limitation of an ssDNA secondary structure on the detection sensitivity is broken through, and a general solution is provided for the detection of complex targets. The detection platform disclosed by the invention shows high specificity on vibrio parahaemolyticus and does not have cross reaction; in practical prawn sample detection, the detection rate reaches 100%, and the result is completely consistent with the qPCR gold standard. The whole detection process can be completed within 2 hours, and a complex DNA purification step is not needed. The collaborative mechanism can be widely applied to detection scenes of other pathogenic bacteria or nucleic acid targets, and has extremely high platform ductility.

Composite amplification system and detection kit for respiratory tract pathogenic bacteria and drug-resistant genes

Resumen de: CN121344222A

The invention belongs to the technical field of molecular detection, and particularly relates to a detection kit for respiratory tract pathogenic bacteria and drug-resistant genes based on a capillary electrophoresis detection platform and application of the detection kit. Compared with a traditional method, the method has the advantages that common respiratory tract infection pathogenic bacteria and drug-resistant genes are covered, detection sites are more comprehensive, specificity is higher, sensitivity is better, reliability is higher, and batch detection capacity is achieved.

Semi-solid culture medium for rapid detection of escherichia coli as well as detection method and application of semi-solid culture medium

Resumen de: CN121344143A

The invention relates to the technical field of Escherichia coli detection, and particularly discloses a semisolid culture medium for rapid detection of Escherichia coli and a detection method and application thereof, the semisolid culture medium comprises the following components by weight: 4.2-5.8 g/L of agar; 8.5 to 11.5 g/L of tryptone; the semi-solid culture medium comprises the following components: 3.2 to 4.8 g/L of beef extract, 6.5 to 9.5 g/L of lactose, 1.2 to 2.2 g/L of D-glucose, 4.2 to 5.8 g/L of sodium chloride, 42000 to 58000 mu g/L of penicillin, 0.12 to 0.28 g/L of cholate, 0.06 to 0.14 g/L of disodium EDTA (Ethylene Diamine Tetraacetic Acid), 0.12 to 0.22 g/L of L-cysteine, 1.2 to 2.8 g/L of yeast extract, 0.005 to 0.015 g/L of vitamin B and an acid-base indicator, the semisolid culture medium and the detection method thereof provided by the invention not only solve the core pain point of the existing escherichia coli detection method in the technical level, but also reduce the operation cost and improve the benefit for enterprises in the economic level, promote the standardization and high efficiency of the food microorganism detection technology in the industry level, and have practicability and industrial popularization value.

Immunomagnetic bead with thermomagnetic dual response, preparation method of immunomagnetic bead and application of immunomagnetic bead in detection of food-borne pathogenic bacteria

Resumen de: CN121347803A

The invention discloses an immunomagnetic bead with thermomagnetic dual response, a preparation method of the immunomagnetic bead and application of the immunomagnetic bead in detection of food-borne pathogenic bacteria. The preparation method comprises the following steps: (1) preparation of the magnetic core; (2) construction of the inorganic shell layer; (3) introduction of the temperature-sensitive polymer layer; (4) surface functionalization and streptavidin coupling; organic combination of the thermal response polymer and the magnetic core-shell structure is realized for the first time, so that the immunomagnetic bead has dual response capabilities of temperature control gathering and scattering and magnetic field separation. Under a low-temperature condition, the magnetic beads are kept in a dispersed state, so that target combination is facilitated; after the temperature is raised, the polymer is subjected to hydrophobic collapse, particles are gathered, and rapid capture is realized through a magnetic field, so that the detection efficiency and the binding stability are greatly improved.

Method for detecting escherichia coli O157 based on CRISPR technology

Resumen de: CN121320581A

The invention provides a method for detecting escherichia coli O157 based on a CRISPR (clustered regularly interspaced short palindromic repeats) technology. The method comprises the following steps: S1, amplifying a sample to be detected to obtain an amplification product; amplification of the to-be-detected sample is to amplify the to-be-detected sample by using the RPA amplification primer group; s2, contacting the amplification product with Cas protein, sgRNA and a single-stranded nucleic acid detector, and detecting a detectable signal generated by cutting the single-stranded nucleic acid detector by the Cas protein, so as to detect the escherichia coli O157, the sequences of the RPA amplification primer group are as shown in SEQ ID No.2 and SEQ ID No.7, and the sequence of a target region hybridized with target nucleic acid in the sgRNA is as shown in SEQ ID No.9. The method disclosed by the invention has the advantages of good inclusiveness, strong specificity, high sensitivity and the like, and has a wide application prospect.

Rapid detection kit and detection method for vibrio parahaemolyticus based on one-tube method RPA-CRISPR/Cas12a

Resumen de: CN121320591A

The invention relates to a rapid vibrio parahaemolyticus detection kit based on a one-tube method RPA-CRISPR/Cas12a and a detection method of the rapid vibrio parahaemolyticus detection kit. A specific RPA (recombinase polymerase amplification) primer and CRISPR crRNA (clustered regularly interspaced short palindromic repeats) are designed by taking a ToxR gene of vibrio parahaemolyticus as According to the established one-tube method RPA-CRISPR/Cas12a detection system, the risk of aerosol pollution caused by a cover opening process is effectively avoided. The method disclosed by the invention is high in specificity, and has no cross reaction with related vibrios such as vibrio vulnificus, vibrio alginolyticus, vibrio cholerae, vibrio harveyi and vibrio anguillarum, and other aquatic pathogenic bacteria such as pseudomonas aeruginosa and pseudomonas plecoglossicida; the sensitivity is high, and the lowest detection limit of genome DNA is as low as 1 pg/mu L; the detection is rapid, and the whole process of nucleic acid amplification and fluorescence detection only needs about 30 minutes. The kit and the method do not need complex instruments and equipment, are simple to operate, are suitable for on-site rapid detection of aquatic product samples in a base layer, and have important application values in early screening, epidemiological investigation and comprehensive prevention and control of vibrio parahaemolyticus.

Method for detecting pathogenic bacteria in toothpaste, method for extracting and purifying nucleic acid from toothpaste and kit and phase change filtering assembly thereof

Resumen de: CN121320585A

The invention relates to the technical field of detection of pathogenic bacteria in oral care products, in particular to a method for detecting pathogenic bacteria in toothpaste, a method for extracting and purifying nucleic acid from the toothpaste and a kit and a phase change filtering assembly thereof. The pathogenic bacterium detection method comprises the following steps: carrying out cracking treatment on a toothpaste sample, and treating the cracked toothpaste sample by using a phase change filtering assembly; carrying out column purification on the dissolved sample subjected to gel filtration to obtain an eluent; and carrying out PCR (Polymerase Chain Reaction) detection on the eluent. According to the technical scheme, the technical problem that in the prior art, a method for rapidly and accurately detecting the microorganisms in the oral care product in an anti-matrix interference mode does not exist can be solved. According to the technical scheme, triple purification of cracking, phase change filtration and column purification is carried out, and a complex toothpaste formula is adapted. Compared with the prior art, the microbiological detection method provided by the technical scheme has the advantages that the detection efficiency, accuracy and suitability are remarkably improved, the microbiological safety of the oral care product is guaranteed, and the method has ideal popularization and application values.

Primer probe set, detection reagent and application of primer probe set in detection of virulent pathogenic bacteria

Resumen de: CN121320595A

The invention relates to the field of biological detection, in particular to a primer probe set, a detection reagent and application of the primer probe set and the detection reagent in detection of virulent pathogenic bacteria. The invention provides a primer probe group. The primer probe group comprises a primer probe group used for amplifying bacillus anthracis, Francisella tularensis, bacillus plague, rhinotrichum-like bacteria, brucella, vibrio cholerae, salmonella typhimurium and staphylococcus aureus, wherein the primer probe group is used for amplifying bacillus anthracis, Francisella tularensis, bacillus plague, rhinotrichum-like bacteria, brucella, vibrio cholerae and salmonella typhimurium. The invention establishes a primer probe combination for rapidly and sensitively detecting various virulent pathogenic bacteria and a kit thereof, aiming at eight virulent pathogenic bacteria such as bacillus anthracis, Francisella tularensis, bacillus plague, rhinotrichum, vibrio cholerae, brucella, salmonella typhimurium and staphylococcus aureus. The kit is stored in a freeze-dried state, adaptive sample lysate can directly expand different types of samples, and the kit has the advantages of being good in specificity, high in sensitivity, short in detection time, good in accuracy, good in stability after redissolution and good in compatibility.

Rapid detection method for listeria monocytogenes RAA-CRISPR/Cas12a

Resumen de: CN121294692A

The invention belongs to the technical field of microbiological detection, and particularly relates to a rapid detection method for listeria monocytogenes RAA-CRISPR/Cas12a and application of the rapid detection method for the listeria monocytogenes RAA-CRISPR/Cas12a. According to the present invention, based on the combination of the recombinase polymerase amplification (RAA) and the CRISPR-Cas12a system, the ultra-sensitive and rapid detection of the Listeria monocytogenes is achieved through the constant temperature nucleic acid amplification and the specific gene recognition; the sensitivity reaches 3.6 CFU/mL (bacterial liquid) and 3.78 copies/L (genome DNA); the detection time is shortened to 26 minutes, and the method can be applied to rapid detection of various environmental samples.

Hybridoma cell strain for detecting Lijiang pseudomonas as well as antibody and application of hybridoma cell strain

Resumen de: CN121294364A

The invention discloses a hybridoma cell strain for detecting Lijiang pseudomonas as well as an antibody and application of the hybridoma cell strain. The hybridoma cell strain comprises a Hybrid cell line FL5391-19 hybridoma cell strain and/or a Hybrid cell line FL5391-14 hybridoma cell strain, and the hybridoma cell strain comprises a hybridoma cell line FL5391-19 hybridoma cell strain and a hybridoma cell line FL5391-14 hybridoma cell strain. The hybridoma cell line FL5391-19 hybridoma cell strain is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: C2025248, and the preservation date is August 13, 2025; the hybridoma cell line FL5391-14 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC (China Center for Type Culture Collection) NO: C2025247, and the preservation date is August 13, 2025. By utilizing the specific monoclonal antibody pair, not only can the Lijiang pseudomonas be detected with high specificity, but also the cichorium intybus pseudomonas and the capsicum pseudomonas with similar genetic relationship can be detected in a cross manner, so that the coverage detection on the main pathogenic bacteria of the tobacco bacterial black spot is realized.

Rapid separation and detection method for pathogenic bacteria in aquaculture water

Resumen de: CN121294686A

The invention discloses a rapid separation and detection method for pathogenic bacteria in a culture water body, and belongs to the field of microbiological detection. According to the method, the sample size is reduced by enriching microorganisms, no complex equipment is needed, and the detection period is remarkably shortened through room-temperature pyrolysis and isothermal amplification LAMP detection, so that the requirements of aquaculture early warning and medication guidance are met; the mutant Bst-WK30 DNA polymerase containing seven specific amino acid mutation sites is adopted for LAMP detection, the amplification efficiency is high, the specificity is high, pathogenic bacteria can be accurately identified, and multiple common pathogenic microorganisms with large harm can be detected. All the components of the kit cooperate to realize efficient splitting decomposition and specific amplification; through cooperation of physical filtration and electrochemical enrichment, the separation device rapidly separates target microorganisms, reduces impurity interference, enriches the microorganisms to a filter membrane within 20 min, and is simple in structure, convenient to carry and suitable for rapid on-site sample treatment.

Primer set for detecting pathogens causing Salmonella Enteritis in pigs and Method for detecting pathogens using the same

Resumen de: KR20260003546A

본 발명은 돼지 살모넬라성 장염 유발 병원균을 검출할 수 있는 프라이머 세트에 관한 것으로, 본 발명에 따른 프라이머 세트를 이용하여 중합효소 연쇄반응을 통해 돼지 살모넬라성 장염 유발 병원균을 신속, 정확하게 검출할 수 있고, 검출한계가 낮아 소량의 돼지 가검물 시료로부터 병원균을 검출하여 감염병을 조기에 진단할 수 있다.

DETECTION OF ENTEROHEMORRHAGIC ESCHERICHIA COLI

Resumen de: EP4674974A1

The invention relates to methods for detecting the presence or absence of an enterohemorrhagic Escherichia coli (EHEC) in a sample and/or identifying said EHEC. The invention further relates to such methods comprising subjecting the sample or protein therein to mass spectrometry to obtain mass spectrometry data, comparing the obtained mass spectrometry data with reference data for at least one EHEC strain with a specific serotype and optionally one or more non-EHEC strains and detecting the presence or absence of the EHEC.

Method for synchronously and quickly detecting various mycotoxins by competitive immunochromatography based on microbial nano-enzyme tag and application thereof

Resumen de: CN121276046A

The invention discloses a method for synchronously and quickly detecting various mycotoxins by competitive immunochromatography based on a microbial nano-enzyme label and application, and the method can be used for simultaneously and accurately detecting various common mycotoxins, including aflatoxin B1, zearalenone and fumonisins B1, by utilizing the enhanced catalytic effect mediated by the microbial nano-enzyme label. The surface of Escherichia coli is coated with a SiO2 interlayer so as to improve the structural stability and the solution dispersity, and meanwhile, the surface of Escherichia coli is coated with a layer of catalytic shell composed of Au (at) Pt nanoparticles so as to generate excellent colorimetric and catalytic performance. According to the application, ESi-Au-Pt is introduced into a nano enzyme-ICA system, so that the sensitivity and accuracy of on-site analysis of a complex sample are remarkably improved, the limit of detection (LOD) of synchronous detection of target fungaltoxin is reduced to 6.1/1.6/5.4 pg/mL, the detection range spans 4-5 orders of magnitude, in addition, the established method shows excellent accuracy and stability in a complex matrix, and the method can be applied to the field analysis of fungaltoxin. The method shows a huge potential in the field-based real-time monitoring of small-molecule pollutants.

Kit for rapidly and jointly detecting multiple pathogen nucleic acids related to dyspnea pregnancy outcome and application of kit

Resumen de: CN121272078A

The invention belongs to the technical field of in-vitro diagnostic reagents for rapid detection of human pathogenic microorganisms, and particularly relates to a kit for rapid combined detection of multiple pathogen nucleic acids related to a dyspnea pregnancy outcome and application of the kit. The pathogen is selected from one or more of escherichia coli, candida albicans, candida glabrata, group B streptococcus, streptococcus gonorrhoeae, chlamydia trachomatis or ureaplasma urealyticum, and the kit comprises a pathogen and lactobacillus rapid detection micro-fluidic chip. A primer group for detecting the pathogen and the lactic acid bacillus nucleic acid and an array consisting of an amplification control and a blank control are fixed on the micro-fluidic chip. According to the present invention, the microfluidic chip rapid detection technology and the nucleic acid amplification technology are adopted to achieve the purposes of rapid detection and multiple detection so as to rapidly and conveniently determine the poor prognosis risk of the pregnant woman at present, timely take the specific measures, and avoid the occurrence of the poor prognosis.

Multiplex PCR (Polymerase Chain Reaction) primer for simultaneously detecting eight respiratory tract pathogenic bacteria and application of multiplex PCR primer

Nº publicación: CN121272080A 06/01/2026

Solicitante:

HEBEI MEDICAL UNIV
\u6CB3\u5317\u533B\u79D1\u5927\u5B66

Resumen de: CN121272080A

The invention belongs to the technical field of bacterium detection, and discloses a multiple PCR primer for simultaneously detecting eight respiratory tract pathogenic bacteria and application thereof. On the basis of a Luminex liquid phase chip technology, specific primers are designed aiming at eight common respiratory tract infection pathogenic bacteria including pseudomonas aeruginosa, staphylococcus aureus, streptococcus pneumoniae, klebsiella pneumoniae, acinetobacter baumannii, stenotrophomonas maltophilia, haemophilus influenzae and escherichia coli; the multiple PCR primers which are high in amplification efficiency, good in specificity and suitable for simultaneously detecting the eight respiratory tract pathogenic bacteria are screened out, the multiple PCR system and microsphere hybridization conditions are continuously optimized, the multiple PCR method for the eight respiratory tract pathogenic bacteria is successfully constructed by applying the Luminex xTAG technology, and the multiple PCR method can be applied to rapid detection of multiple pathogenic microorganisms.