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Salmonella dual-mode detection method based on medium-temperature Argonaute driving

Resumen de: CN122409630A

The invention discloses a salmonella dual-mode detection method based on medium-temperature Argonaute driving, and belongs to the technical field of food safety detection. According to the method, the functionalized DNA hydrogel internally packaged with an asymmetric RPA system, a CbAgo system and nano-enzyme is constructed, cascade signal amplification, DNA hydrogel phase change and nano-enzyme signal output links are integrated, and rapid detection of salmonella is realized under the assistance of gravity. The invention also independently develops portable detection equipment and an intelligent application program, realizes automatic image acquisition and analysis, has the advantages of high sensitivity, strong specificity and simplicity and convenience in operation, and is suitable for on-site rapid detection of food safety.

Specific primer combination for detecting glacier melt water transmission related pathogenic microorganisms

Resumen de: CN122405852A

The invention relates to a specific primer combination for detecting glacier melt water transmission related pathogenic microorganisms. The specific primer combination comprises a vibrio cholerae primer Seq1 with a target gene of ompW, a bacillus cereus primer Seq4 with a target gene of gyrB, a clostridium botulinum primer Seq7 with a target gene of bond/A, and a legionella pneumophila primer Seq10 with a target gene of mip. The method is high in sensitivity and good in environmental applicability, not only can realize joint detection of spreading related pathogenic microorganisms in the glacier environment, but also can be used for analyzing migration and diffusion paths in different environmental media, and provides technical support for glacier melt water spreading process and downstream biological safety risk assessment.

Detection system for food-borne pathogenic bacteria and application thereof

Resumen de: CN122405789A

The invention belongs to the technical field of biological detection, and particularly relates to a detection system for food-borne pathogenic bacteria and application of the detection system. The detection system for the food-borne pathogenic bacteria comprises a composite response material, hyaluronic acid hydrogel containing L-ascorbic acid-2-trisodium phosphate and bacteriophage, the composite response material is prepared from ZIF-90 nano particles, hyaluronidase and alkaline phosphatase, wherein the hyaluronidase and the alkaline phosphatase are encapsulated in the ZIF-90 nano particles. According to the invention, a dual-mode paper-based visual detection system based on combination of phage specific recognition and a ZIF-90 response release mechanism is constructed, and high-specificity detection of target pathogenic bacteria is realized. Compared with a nucleic acid amplification or immunological method which has the defect that live bacteria and dead bacteria are difficult to distinguish, the method provided by the invention only generates signal response to the live bacteria which can be infected by the bacteriophage, so that the authenticity and the reliability of a detection result are remarkably improved.

Quadruple PCR (Polymerase Chain Reaction) detection kit and method for detecting four bovine-derived respiratory pathogenic bacteria

Resumen de: CN122405853A

The invention belongs to the technical field of biological detection, and discloses a quadruple PCR (Polymerase Chain Reaction) detection kit and method for detecting four bovine-derived respiratory tract pathogenic bacteria. The kit and the method comprise a primer pair for detecting pasteurella multocida with nucleotide sequences shown as SEQ ID NO.1 and SEQ ID NO.2, a primer pair for detecting mannheimia haemolytica with nucleotide sequences shown as SEQ ID NO.3 and SEQ ID NO.4, and a primer pair for detecting mannheimia haemolytica with nucleotide sequences shown as SEQ ID NO.3 and SEQ ID NO.4, the nucleotide sequences of the primer pair are as shown in SEQ ID NO.5 and SEQ ID NO.6, and the primer pair is used for detecting klebsiella pneumoniae; the nucleotide sequences of the primer pair are as shown in SEQ ID NO.7 and SEQ ID NO.8, and the primer pair is used for detecting mycoplasma bovis. The quadruple PCR detection kit and the quadruple PCR detection method provided by the invention can amplify DNAs of four pathogens including Pm, Mh, Kp and MB in the same reaction system, and have the characteristics of high detection efficiency, high sensitivity and strong specificity.

Method, composition and kit for rapidly detecting two food-borne pathogenic pseudomonas

Resumen de: CN122405858A

The invention relates to an oligonucleotide primer probe composition for detecting food-borne pathogenic pseudomonas bacteria (pseudomonas alcaligenes and pseudomonas buchneri) based on an ERA fluorescence method or an ERA test strip method, and a detection method using the composition. The pseudomonas alcaligenes or pseudomonas buchneri in the sample can be rapidly, specifically and sensitively detected, and the method is easy and convenient to operate, short in reaction time and free of complex temperature control equipment, and can be widely applied to rapid screening, pollution monitoring and risk early warning of food-borne pathogenic pseudomonas in various food samples such as milk and dairy products.

Method for rapidly detecting streptococcus suis or/and salmonella based on RAA-EXO technology and application thereof

Resumen de: CN122382222A

The invention discloses a method for rapidly detecting streptococcus suis or/and salmonella based on an RAA-EXO technology and application of the method. According to the method, specific RAA primers and EXO probes are designed and screened aiming at streptococcus suis recN genes and salmonella invA genes, the sequences of the specific RAA primers and the EXO probes are shown as SEQ ID NO.3, 5 and 10 and SEQ ID NO.14, 15 and 20 respectively, a single or double RAA-EXO detection system for the streptococcus suis and the salmonella is established, detection can be completed within 20 minutes under the condition of the constant temperature of 39-41 DEG C, and the detection sensitivity is high. And no cross reaction with common bacteria such as escherichia coli, staphylococcus aureus and the like exists. Results can be visually judged through a fluorescence curve or a blue light instrument, an effective tool is provided for rapid, sensitive and on-site detection of streptococcus suis and salmonella, and the kit has important application value in the fields of food safety and epidemic disease prevention and control.

Salmonella detection system based on RPA-CRISPRCas12a and application thereof

Resumen de: CN122382226A

The invention discloses a salmonella detection system based on RPA-CRISPRCas12a and application of the salmonella detection system. The detection system comprises an RPA (recombinase polymerase amplification) primer pair, a probe, crRNA (ribonucleic acid) and LbCas12a protein, the sequences of the RPA amplification primer pair are as shown in SEQ ID NO. 1 and SEQ ID NO. 2; the sequence of the probe is as shown in SEQ ID NO.7; and the sequence of the crRNA is as shown in SEQ ID NO. 8. The RPA-CRISPR/Cas12a visual detection system has the advantages of high sensitivity, strong specificity, simplicity and convenience in operation, rapidness in detection and the like, the lowest detection limit of salmonella reaches 1 fg/mu L, and stable and repeatable fluorescence signals and visual results can still be generated under the concentration. The system effectively overcomes the defects of a traditional detection method, is suitable for on-site rapid screening and risk early warning of salmonella in a food chain, provides technical support for food safety supervision, and also provides a reference technical scheme for rapid detection of other food-borne pathogenic bacteria.

CRISPR-Cas12a-based rapid detection method for food-borne pathogenic bacteria

Resumen de: CN122382221A

The invention relates to the technical field of nucleic acid detection, in particular to a food-borne pathogenic bacterium rapid detection method based on CRISPR-Cas12a. According to the method, after genome DNA is extracted from a food sample, bridging primers of an upstream primer combination identification gene segment and a downstream primer combination virulence gene segment are provided, the two segments are located on the same continuous DNA molecule at the interval of 50-1500 bp, and the two segments are located on the same continuous DNA molecule; amplifying for 20-40 minutes at the constant temperature of 37 DEG C, and amplifying to obtain a chimeric amplicon only when the two segments are linked to the same molecule; and activating Cas12a trans-cleavage activity by combining crRNA of a guide sequence spanning a junction site with a chimeric amplicon, cleaving a single-stranded DNA reporter probe and detecting a signal. In the single-tube constant-temperature reaction, ligase is not needed, the linkage state of the gene and the virulence gene is directly confirmed and identified, the detection limit is 10-100 CFU/mL, and the time consumed in the whole process is less than 60 minutes.

Super-multiplex PCR (polymerase chain reaction) primer combination capable of simultaneously detecting 35 pathogenic bacteria and fecal indicator bacteria and application of super-multiplex PCR primer combination

Resumen de: CN122382225A

The invention discloses a super-multiplex PCR (polymerase chain reaction) primer combination capable of simultaneously detecting 35 pathogenic bacteria and fecal indicator bacteria and application of the super-multiplex PCR primer combination. The super-multiplex PCR primer combination comprises 35 pairs of target specific primers, and nucleotide sequences of the 35 pairs of target specific primers are shown as SEQ ID NO.1-70. The method for detecting 35 pathogenic bacteria and fecal indicator bacteria comprises the following steps: extracting DNA of a sample to be detected to obtain a DNA mixture of a target sample; carrying out ultra-multiplex PCR amplification on the target sample DNA mixture to obtain a target amplification product, and carrying out high-throughput sequencing to obtain a target amplification product sequencing result; and detecting and judging the to-be-detected sample. According to the method, generation of dimers among the primers and nonspecific amplification are effectively reduced, homogenized amplification of 35 targets in a single reaction system is realized, and the method can be applied to the fields of food-borne pathogenic bacterium detection, public health emergency detection and the like.

Composite probe and test strip for detecting food-borne pathogenic bacteria and application of composite probe and test strip

Resumen de: CN122385882A

The invention provides a composite probe and a test strip for detecting food-borne pathogenic bacteria and application of the composite probe and the test strip, and belongs to the technical field of food-borne pathogenic bacteria detection. The composite probe comprises a bacteriophage functional capture probe and a ZMPAP signal composite probe; the bacteriophage functional capture probe contains a specific inactivated bacteriophage aiming at the food-borne pathogenic bacteria; the ZMPAP signal composite probe is prepared from a ZIF-67 metal organic framework material, methylene blue, polydopamine, gold nanoparticles and porcine immune globulin G. Compared with a single signal detection technology, the method disclosed by the invention has visual qualitative and accurate quantitative capabilities, is strong in specificity and good in repeatability, and provides a core technical support for on-site rapid prevention and control of the food-borne pathogenic bacteria.

Multiple Raman/visual dual-mode detection method and kit for pathogenic bacteria based on Cas12a cis/trans-cleavage activity

Resumen de: CN122385571A

The invention discloses a single-tube multiple Raman/visual detection method and kit for pathogenic bacteria based on Cas12a and an in-situ crRNA synthesis strategy, and belongs to the technical field of food safety detection. By designing single-molecule allosteric hairpin DNA containing a target recognition region, a T7 promoter and a crRNA template, 16S rRNA of specific response target bacteria, hairpin structure recombination and crRNA in-situ synthesis are started, and cis-and trans-cleavage of Cas12a is guided, so that rapid visual detection of the total bacteria amount and SERS identification and quantitative detection of different pathogenic bacteria targets are realized. The method solves the problem of insufficient qualitative accuracy and quantitative sensitivity caused by the interference of a biological matrix in the existing rapid detection method of the pathogenic bacteria, overcomes the dependence of a CRISPR/Cas12a method on a target PAM site, and greatly widens the detection range of the nucleic acid target of the food-borne pathogenic bacteria. The Raman/visual dual-mode detection system adapts to a field rapid detection scene, the detection limit is as low as the single cell level, the specificity and reproducibility are excellent, and the Raman/visual dual-mode detection system can be used for scenes such as food safety control and live pathogenic bacterium diagnosis.

O45 Escherichia coli detection antibody and application thereof

Resumen de: CN122356274A

The invention relates to the field of biological medicine, in particular to an O45 Escherichia coli detection antibody and an application method thereof. The antibody is a monoclonal antibody or an antigen binding fragment thereof, and comprises a heavy chain CDR sequence as shown in SEQ ID NO: 1-3 and a light chain CDR sequence as shown in SEQ ID NO: 4-6. The invention also provides a nucleic acid molecule for coding the antibody, an expression vector, a host cell and a preparation method thereof. Specific identification results show that the antibody is specifically combined with O45 Escherichia coli, has no cross reaction with O157, O26, O103, O111, O121, O145 and other Escherichia coli serotypes and O55 related serotypes, and also has no cross reaction with common food-borne pathogenic bacteria. The detection limit of an indirect ELISA detection method established based on the antibody to O45 Escherichia coli can reach 1 * 10 < 2 > CFU/mL, and the whole detection process can be completed within 24 hours. The monoclonal antibody provided by the invention can be applied to preparation of detection kits, detection test paper and other products, and is suitable for rapid screening of O45 Escherichia coli in import and export food.

DETECTION OF ATYPICAL PNEUMONIA

Resumen de: EP4772522A2

Disclosed herein are methods and compositions for detecting one or more pathogens that cause atypical pneumonia. Detectable pathogens include Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila.

Fluorescent rapid detection reagent and application thereof

Nº publicación: CN122344612A 07/07/2026

Solicitante:

FUDAN UNIV
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Resumen de: CN122344612A

The invention provides a fluorescent rapid detection reagent and application thereof, and belongs to the technical field of bacterial detection. The rapid fluorescent detection reagent provided by the invention comprises a bacterium specific aptamer, an initiation chain and hairpin structures H1, H2 and H3. By establishing a fluorescent specific amplification detection system with strong specificity and high sensitivity, a fluorescent signal amplification marker aiming at staphylococcus aureus and pseudomonas aeruginosa susceptible to rodents and a handheld visual detection technology of the fluorescent signal amplification marker are developed, and the fluorescent signal amplification marker and the handheld visual detection technology are prepared into a detection reagent, so that rapid and sensitive detection of target bacteria is realized, and the detection efficiency is improved. The method has important significance on screening detection and quality control of pathogenic microorganisms of experimental animals.