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三级淋巴结构生物模型的构建方法

Resumen de: CN122081186A

本发明公开了一种三级淋巴结构生物模型的构建方法，可以快速高效诱导原位TLS的产生，不依赖于特定的组织器官，不需要细胞编辑、转基因小鼠等复杂昂贵的制备流程，在2～3周内产生稳定、成熟、存续时间长的TLS结构，具有周期短、成本低、操作简单、可重复、安全有效的特点。

METHODS OF PRODUCING SHIGA TOXIN B-SUBUNIT (STXB) MONOMERS AND OLIGOMERS, AND USES THEREOF

Resumen de: US20260137765A1

A method of producing a monomer of a Shiga toxin B-subunit (STxB) protein or of a variant thereof by peptide chemical synthesis, as well as to a method of producing a pentamer of the STxB protein or of the variant thereof. The methods are particularly advantageous as they overcome major issues typically observed in peptide chemical synthesis, including solubility and purity issues.

Novel Salmonella Enterica-Specific Bacteriophage OPT-SAL01, and Antibacterial Composition Comprising Same

Resumen de: US20260137738A1

The present invention relates to a novel bacteriophage OPT-SALO1 with specific killing ability for Salmonella enterica, an antibiotic composition comprising the bacteriophage, a composition for adding to a feed, a feed, a disinfectant or a cleaning agent, and a method for preventing or treating infectious diseases caused by Salmonella enterica comprising a step of administering the bacteriophage to a subject.

Burkholderia pseudomallei complex outer membrane vesicles as adjuvants

Resumen de: AU2026203336A1

Outer membrane vesicles from bacteria of the Burkholderia pseudomallei complex can be used as adjuvants in compositions and methods to potentiate the immune response to immunogens. ay a y

METHOD FOR INCREASING ETEC CS6 ANTIGEN PRESENTATION ON CELL SURFACE AND PRODUCTS OBTAINABLE THEREOF

Resumen de: US20260139012A1

A method for increasing the presentation of ETEC CS6 antigen on a cell surface, comprising the step of contacting cells expressing said antigen with an aqueous solution comprising 0.6-2.2 percent phenol by weight, such that the presentation of said antigen is increased by at least 100%. A method for the manufacture of a killed whole cell vaccine for immunization against CS6-expressing ETEC. Cells and vaccines obtainable by the above methods.

ASSAYS FOR ANTIMICROBIAL ACTIVITY AND APPLICATIONS THEREOF

Resumen de: US20260140114A1

The disclosure provides methods, compositions, and kits for enhanced detection of microbes in samples and monitoring of antimicrobial activity in a subject.

Detoxified lipopolysaccharides (LPS), naturally non-toxic LPS, and uses thereof

Resumen de: AU2026203393A1

DETOXIFIED LIPOPOLYSACCHARIDES (LPS), NATURALLY NON-TOXIC LPS, AND USES THEREOF The invention relates to an enriched population of modified lipopolysaccharide (LPS) molecular species being: - devoid of phosphate group at position C1 of the reducing end of their lipid A domain; and - substituted at position C6’ of the non-reducing end of their lipid A domain by a 10 hydrophilic moiety, with the proviso that said hydrophilic moiety is not a hydroxyl group. It also relates to compositions comprising the enriched population of modified LPS; and uses of naturally-occurring LPS molecular species and/or enriched population of modified LPS molecular species for treating and/or preventing cancer, inflammatory 15 diseases or infectious diseases, and for stimulating an immune response or vaccinating a subject. - 10 - ay a y - - 1

ANTI-INTERLEUKIN-23 RECEPTOR VHH ANTIBODIES AND USES THEREOF

Resumen de: WO2026102349A1

Interleukin-23 receptor (IL23R) VHH antibodies are disclosed, as well as methods of making and using the same, e.g., for treatment of IL23-mediated diseases and disorders, including, e.g., gastrointestinal-related (Gl-related) diseases (e.g., inflammatory bowel disease (IBD) (e.g., ulcerative colitis (UC) or Crohn's disease (CD)), a colon cancer, a small intestine cancer, a gastric cancer, an irritable bowel syndrome, a gastrointestinal ulcer, a gut-associated infection (e.g., a Salmonella infection or a Clostridium difficile infection), celiac disease, or pathogenic inflammation).

USE OF SALMONELLA TYPHIMURIUM-DERIVED GLUTAMATE DEHYDROGENASE AND RELATED BIOMATERIALS THEREOF IN AMINO ACID SYNTHESIS

Resumen de: WO2026098490A1

Disclosed in the present application is the use of Salmonella typhimurium-derived glutamate dehydrogenase and related biomaterials thereof in amino acid synthesis. Provided in the present application is the use of Salmonella typhimurium-derived glutamate dehydrogenase for improving the L-amino acid yield of a microorganism. In the present application, an optimized coding gene of the Salmonella typhimurium-derived glutamate dehydrogenase is further obtained by means of codon optimization, and the introduction of the optimized coding gene into a recipient microorganism can significantly improve the L-amino acid yield of the recipient microorganism.

Methods of Inactivating Pathogens

Resumen de: US20260131039A1

This disclosure relates to a method for sanitizing a space. The method includes applying a composition containing triethylene glycol into a space containing a pathogen (e.g., a Staphylococcus, a Pseudomonas, a Listeria, Salmonella, Klebsiella, a mycobacterium, a mold, or a spore) in an amount effective to inactivate the pathogen.

SIGNAL SEQUENCES FOR DELIVERING CARGO TO BACTERIAL MICROCOMPARTMENTS

Resumen de: US20260132409A1

The present disclosure provides fusion proteins comprises fusion proteins comprising the signal sequence of PduB or PduM and a heterologous protein, as well as constructs for expressing the fusion proteins, and methods of their use. The fusion proteins are designed to deliver the heterologous proteins to bacterial microcompartments and modify the 1,2-propanediol metabolic pathway.

APPARATUS AND METHOD FOR DETECTING MICROBIAL CONTAMINATION

Resumen de: WO2018208840A1

Provided are novel methods for screening and testing for pathogens in food, water, and bodily fluids using methods that are faster to complete than conventional methods of culturing and plating that require lengthy times in properly equipped labs. The invention utilizes specific, rapid and sensitive optical detection to capture small concentrations of the target bacteria and render them amenable for detection with various specific synthesis binding agents approaches. The technique merges capture and detection steps with quantification unit suitable to provide results in a relatively shorter time current detection methods.

一种基于SERS的快速超灵敏检测大肠杆菌O157:H7的方法

Resumen de: CN121995052A

0001 本发明属于生物检测技术领域，具体公开了一种基于SERS的快速超灵敏检测大肠杆菌O157:H7的方法，包括如下步骤：制备免疫磁珠捕获纳米粒子、制备贵金属或贵金属复合纳米粒子溶胶、制备免疫贵金属或贵金属复合信号纳米粒子、制备三明治式夹心结构、大肠杆菌O157:H7检测。本发明的方法以抗体作为特异性识别元件，同时特异性结合并测定大肠杆菌O157:H7，一方面能够稳定纳米材料在检测体系中的分散状态，另一方面能够高特异性和高亲和力地识别目标蛋白，并且稳定性好，制备成本低，在很大程度上提高了检测的准确性；本发明检测速度更快本发明检测流程极简，用时短，适配现场快检与批量筛查，同时检出限低，兼具超快检测速度与超高检测灵敏度，可精准识别痕量目标菌体。

METHODS OF TREATMENT OF INTESTINAL INFECTIONS

Resumen de: US20260124215A1

The present invention relates to compositions and methods for treating or preventing infections, in particular infections by intracellular parasites such as Cryptosporidium spp. The present invention also relates to compositions and methods for treating or preventing viral or bacterial infections, in particular intestinal infections such as those caused by rotavirus and Salmonella spp. Infections. The methods comprise administration of, and the compositions comprise, a farnesyl-diphosphate farnesyltransferase 1 (FDFT1) inhibitor, such as lapaquistat.

Salmonella vectored therapies for treatment of cancer

Resumen de: US20260124255A1

0000 A genetically modified Salmonella cell (GMSC) engineered to display pattern-associated and danger-associated molecular patterns to recruit and enhance innate immunity and exhibit specific targeting to cells and regulated delayed lysis in vivo, the GMSC comprising a first heterologous nucleic acid that encodes a first gene product that causes the GMSC to be selectively localized to and/or internalized by a target cell in vivo and a second heterologous nucleic acid that encodes a second gene product that facilitates killing of the target cells following internalization.

REACTION MEDIUM AND METHOD FOR DETECTING SHIGA TOXIN-PRODUCING E.COLI AND/OR ENTEROHAEMORRHAGIC E.COLI

Resumen de: WO2025003117A1

The present invention relates to a gelled reaction medium for detecting, identifying, and/or isolating at least one Shiga toxin-producing strain of E. coli, the reaction medium comprising: - at least one toxin inducer, - at least one agglutinating conjugate comprising at least one specific binding partner of STX1 and/or at least one specific binding partner of STX2, coupled to a nanoparticle; - a concentration gradient of a compound for inhibiting non-target bacteria. The present invention also relates to the associated method for detecting and/or isolating Shiga toxin-producing E. coli which is likely to be present in a sample comprising enterobacteria.

METHOD FOR DETECTING AND CONFIRMING SHIGA TOXIN-PRODUCING ESCHERICHIA COLI AND/OR ENTEROHAEMORRHAGIC E. COLI

Resumen de: WO2025003119A1

The invention relates to a method for detecting and confirming at least one Shiga toxin-producing Escherichia Coli (STEC) which may be present in a sample comprising enterobacteria, comprising the following steps: - performing lysis of the sample, enabling lysis of the STECs in order to obtain a solution comprising the nucleic acids thereof; - bringing the solution of nucleic acids into contact with primers, making it possible to amplify at least the stx1 and/or stx2 gene or gene fragment; - if at least one of the stx1 and/or stx2 genes or gene fragments is amplified, part of the sample is deposited on an agar reaction medium comprising ■ at least one toxin inducer, ■ at least one agglutinating conjugate formed by at least one binding partner specific to the STX1 protein and/or at least one binding partner specific to the STX2 protein, which binding partner(s) is (are) coupled to a nanoparticle; - detecting and confirming the presence of at least one STEC by the appearance of a halo on the agar around the STEC.

用于多模式分泌新抗原的基因改造细菌

Resumen de: WO2025037284A2

A vaccine and methods of treatment thereof, wherein the vaccine comprises a recombinant Gram-negative bacteria genetically modified to express a first antigen fusion peptide comprising a neoantigen or series thereof, said neoantigen or series thereof associated with a first secretion signal from a double membrane-spanning secretion system and a second antigen fusion peptide comprising a homologous neoantigen or series thereof, associated with a second secretion signal from an outer membrane-spanning secretion system. The Gram-negative bacteria may be further modified for quadmodal transport. Specifically, the fusion peptides include signal peptides are each associated with a Type III (T3SS) and a Type V (T5SS) secretion system.

METHODS AND SYSTEMS FOR THE RAPID DETECTION OF SALMONELLA USING INFECTIOUS AGENTS

Resumen de: MX2025013800A

Disclosed herein are methods and systems for rapid detection of microorganisms in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Salmonella-specific bacteriophage, allows detection of a specific microorganism, such as Salmonella spp. and an indicator signal may be amplified to optimize assay sensitivity.

Application of saliva combined lactobacillus in preparation of product for inhibiting growth and proliferation of bacteria and/or improving flavor

Resumen de: CN121942903A

The invention discloses application of lactobacillus salivarius with bacteriostatic ability and capable of improving flavor of fermented milk in the fermented milk, and belongs to the technical field of microorganisms. The fermented milk prepared from the lactobacillus salivarius combined CCFM1332 can inhibit porphyromonas gingivalis, has a strong inhibition effect on the forming ability of salmonella typhimurium, staphylococcus aureus, pseudomonas aeruginosa and pseudomonas fluorescens biological membranes, and increases the content of essential amino acids and the content of flavor amino acids in the fermented milk; the proportion of bitter amino acids is reduced and the flavor of the fermented milk is improved. In addition, the CCFM1332 is high in acid production capacity, the pH can be rapidly reduced, the fermentation time is shortened, and the production efficiency is improved.

Application of phytobacterium plantarum in preparation of product for inhibiting growth and proliferation of bacteria and/or improving flavor

Resumen de: CN121942904A

The invention discloses a plant lactobacillus CCFM1296 strain for improving free amino acid content and in-vitro bacteriostatic ability of fermented milk, and belongs to the technical field of microbial lactobacillus. According to the fermented milk prepared by the plant lactobacillus CCFM1296, the content of free amino acid can be increased, the content of flavor amino acid can be increased, the loss of sweet amino acid can be reduced, and the inhibition effect on the biofilm forming ability of salmonella typhimurium, pseudomonas aeruginosa and pseudomonas fluorescens can be enhanced.

Method for detecting food-borne pathogenic bacteria based on Argonaute protein in combination with loop-mediated isothermal amplification and electrochemical biosensing

Resumen de: CN121951013A

The invention discloses a method for detecting food-borne pathogenic bacteria based on Argonaute protein in combination with loop-mediated isothermal amplification and electrochemical biosensing, and belongs to the technical field of microbiological detection and biosensing. The integrated detection method based on Argonaute protein, loop-mediated isothermal amplification and electrochemical biosensing, which is high in sensitivity, strong in specificity, simple and convenient to operate and suitable for on-site rapid detection, is provided aiming at the problems that an existing food-borne pathogenic bacterium detection method is insufficient in sensitivity, depends on complex instruments, is difficult to apply on site and the like. Target nucleic acid is subjected to exponential amplification through LAMP (loop-mediated isothermal amplification), then Argonaute-mediated two-stage specific recognition and signal transduction are performed, and finally reading is performed through a high-sensitivity electrochemical platform, so that the detection limit of salmonella typhimurium as high as 1 CFU/mL is realized, and the detection range can cover 100-108 CFU/mL.

Rapid detection kit for bacterial food-borne pathogenic microorganisms and application of rapid detection kit

Resumen de: CN121951093A

The invention relates to the technical field of biology, in particular to a rapid detection kit for bacterial food-borne pathogenic microorganisms and application of the rapid detection kit. The invention develops a primer probe combination kit for detecting food-borne bacteria salmonella, staphylococcus aureus, vibrio parahaemolyticus, listeria monocytogenes, escherichia coli O157 and clostridium perfringens through optimization of primers, optimization of hydrogel components and a system and the like. The primer probe combination kit is used for detecting food-borne bacteria salmonella, staphylococcus aureus, vibrio parahaemolyticus, listeria monocytogenes, escherichia coli O157 and clostridium perfringens. The kit is used for detecting salmonella, staphylococcus aureus, vibrio parahaemolyticus, listeria monocytogenes, Escherichia coli O157 and clostridium perfringens. The kit is high in sensitivity and specificity, strong in anti-interference capability, excellent in stability and portability, rapid and efficient in detection, simple and convenient to operate and low in cost, and can realize result visualization and relative quantification, so that the kit has wide application prospects and values.

Application, screening method and medicine of Brawler's bacteria

Resumen de: CN121943972A

The invention belongs to the technical field of biology, and discloses application, a screening method and a medicine of Brawler's bacteria, and application of the Brawler's bacteria to preparation of anti-inflammatory drugs. Firstly, it is determined that intestinal microorganisms play an important role in resisting salmonella infection through a fecal microorganism transplantation test; secondly, screening is carried out on excrement extracts of chicken infected with salmonella, it is found that the abundance of the Brawler is extremely high, and it is further found through in-vitro tests and in-vivo tests that the Brawler can inhibit the systemic inflammatory response, caused by infection of salmonella enteritidis, of the chicken.

Bacillus velezensis JL40 with antibacterial and preservative effects

Nº publicación: CN121950636A 01/05/2026

Solicitante:

FOSHAN UNIV
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Resumen de: CN121950636A

The invention discloses bacillus velezensis JL40 with antibacterial and preservative effects, and belongs to the field of microorganisms. The strain has good biological safety, and antibacterial substances produced by metabolism of the strain can keep stable antibacterial activity under extreme pH, high temperature and various organic solvent environments. Experiments show that the JL40 has a remarkable inhibition effect on food-borne pathogenic bacteria such as escherichia coli, staphylococcus aureus, salmonella and listeria monocytogenes, and meanwhile, the JL40 shows a relatively strong inhibition capability on main pathogenic bacteria such as edwardsiella tarda, aeromonas hydrophila and citrobacter buchneri in aquaculture. In the aspect of agricultural application, infection of banana fruit putrefying bacteria can be reduced, and banana decay is reduced. Therefore, the bacillus velezensis JL40 and the metabolites thereof have wide application potential in the aspects of replacing traditional chemical preservatives, inhibiting food-borne pathogenic bacteria and putrefying bacteria, prolonging the preservation time of the picked bananas and the like.