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一种CeO2-Au复合材料结合TDN-MultiApt形成的MNP-DNA-Apt复合体及制备方法与应用

Resumen de: CN121142039A

本发明公开了一种CeO2‑Au复合材料结合TDN‑MultiApt形成的MNP‑DNA‑Apt复合体及制备方法与应用，所述的复合体是通过下述方法制备得到的：先构建立方体c‑CeO2‑Au纳米颗粒，再构建DNA‑CeO2‑Au探针（DA探针），然后利用四个单链DNA样品构建TDN结构，并通过碱基互补配对与适配体Apt链接，形成TDN‑MultiApt；TDN‑MultiApt通过抗原‑抗体反应和碱基互补配对分别与链霉亲和素修饰的磁珠MNP和DA探针结合形成MNP‑DNA‑Apt复合体，即形成MDA探针。本发明中的立方体CeO2‑Au结合TDN‑MultiApt比色传感系统突破了传统的基于培养和其他分子诊断技术的局限性，该系统通过比色模式进行检测，适用于现场快速检测，突出了其作为现场检测解决方案的潜力。

使用减毒鼠伤寒沙门氏菌治疗神经系统肿瘤

Resumen de: WO2024197078A2

Provided herein are compositions and methods for treating benign nervous system tumors, including schwannomas, using attenuated mutants of Salmonella typhimurium comprising a spiC deletion, and, optionally, one or more checkpoint inhibitors.

COMPOUNDS FOR TREATING INFECTIONS

Resumen de: US2025375435A1

Disclosed herein are compositions and methods for treating a Salmonella infection. Disclosed herein are compositions and methods for inhibiting FraB in Salmonella. Disclosed herein are methods of increasing 6-phospho-fructose-aspartate within Salmonella bacteria.

ANALYTICAL METHOD FOR GLYCOGONJUGATES USING A CAPILLARY-BASED IMMUNOASSAY SYSTEM

Resumen de: US2025377362A1

The invention provides analytical methods for identifying and quantifying complex glycoconjugate compositions, in particular for the analysis of a glycoconjugate in a sample comprising at least 4 glycoconjugates.

약학적 조성물 및 이의 제조 방법과 용도

Resumen de: US20260048111A1

The present disclosure relates to the technical field of medicine, and in particular to a pharmaceutical composition, preparation method and use thereof. The pharmaceutical composition includes a first active ingredient, a second active ingredient, and a pharmaceutically acceptable carrier or excipient. The first active ingredient is a microbial agent, including one or more of Staphylococcus aureus, Bordetella pertussis, diphtheria toxoid, tetanus toxoid, Salmonella typhi, and Salmonella paratyphi. The second active ingredient includes polyinosinic acid, polycytidylic acid, and vitamin. The pharmaceutical composition of the present disclosure pertains to an artificial active immunotherapy for tumors. It can “stimulate” the entire immune system, making the therapy of using bacteria to activate the human immune system to kill cancer cells highly stable and reliable. This composition can significantly save and prolong the lives of cancer patients while exhibiting extremely high safety, minimal toxic side effects, and low production costs.

METHOD FOR ENHANCING IMMUNOGENICITY OF RECOMBINANT PROTEIN ANTIGEN AND USE THEREOF

Resumen de: WO2025246011A1

Provided are a method for enhancing the immunogenicity of a recombinant protein antigen and use thereof. The method comprises conjugating a polysaccharide with a recombinant protein antigen to form a nano-scale protein antigen. The polysaccharide is selected from sodium hyaluronate, chitosan, glucan, fucoidan, and sodium alginate. The recombinant protein antigen is a protein antigen derived from a bacterium. The bacterium belongs to the genus of Staphylococcus, Neisseria, Klebsiella, Escherichia, Clostridium, Salmonella, Shigella, Pseudomonas, Acinetobacter, Bordetella, Enterococcus, Haemophilus, Mycobacterium, or Streptococcus. The method not only improves the immunogenicity of the recombinant protein antigen, but also overcomes the defects of competitive immunoregulation and poor immune enhancement effects caused by bacterial capsular polysaccharide modification. The method also has the advantages of high clinical application value, simple starting material acquisition, low cost, good process stability, ease in scale expansion, high safety, and the like.

METHOD FOR IDENTIFYING A MULTI-PARAMETER PHENOTYPE OF MICROBIOTA

Resumen de: WO2024156739A1

The invention relates to a method for identifying a multi-parameter phenotype of microbiota. The method comprises (i) providing a sample comprising microbiota, (ii) labeling said microbiota with multiple labels, each of which binds a phenotypic parameter of said microbiota, (iii) detecting an intensity of the labelled phenotypic parameters of single cells of the microbiota by flow cytometry, and (iv) segmenting the single cells into bins based on the intensities of detected phenotypic parameters, wherein the distribution of single cells in bins represents a multi-parameter phenotype of said microbiota. The invention further relates to a system for identifying a multi-parameter phenotype of intestinal microbiota, a kit for identifying a multi-parameter phenotype of intestinal microbiota and methods for diagnosing a medical condition associated with microbiota, for example an inflammatory condition, such as an inflammatory bowel disease, in a subject.

一种冰箱沉降菌检测方法及检测装置

Nº publicación: CN121049237A 02/12/2025

Solicitante:

山东省血液中心

Resumen de: CN121049237A

本发明公开一种冰箱沉降菌检测方法及检测装置，涉及检测技术领域，技术方案为，先采用Aptamer和Toehold switch策略构建检测载体作为检测介质；再将其置于待检测空间，形成固定形状和面积的检测区域并与空气接触；接着对检测区域进行图像采集，识别颜色变化区域；最后分析图像获取沉降菌检测结果。此方法无需复杂培养分离，简化流程、缩短检测时间；通过图像采集与颜色分析直观快速出结果，操作便捷。相比传统方法，它克服了费时费力、需多法结合的弊端，能快速准确检测医用冰箱沉降菌，预防交叉感染，保障药品、血液安全，具有高特异性、灵敏度，应用潜力大。