Alerta

GENETICALLY MODIFIED BACTERIA FOR MULTI-MODAL SECRETION OF A NEOANTIGEN

Resumen de: WO2025037284A2

A vaccine and methods of treatment thereof, wherein the vaccine comprises a recombinant Gram-negative bacteria genetically modified to express a first antigen fusion peptide comprising a neoantigen or series thereof, said neoantigen or series thereof associated with a first secretion signal from a double membrane-spanning secretion system and a second antigen fusion peptide comprising a homologous neoantigen or series thereof, associated with a second secretion signal from an outer membrane-spanning secretion system. The Gram-negative bacteria may be further modified for quadmodal transport. Specifically, the fusion peptides include signal peptides are each associated with a Type III (T3SS) and a Type V (T5SS) secretion system.

Bacillus subtilis BS-1 and application thereof in buffalo silage

Resumen de: CN122256177A

The invention relates to the technical field of microorganisms, in particular to bacillus subtilis BS-1 and application of the bacillus subtilis BS-1 in buffalo silage, the strain BS-1 has good bacteriostatic activity on escherichia coli, salmonella and streptococcus uberis, and after the strain BS-1 is mixed with orange peel and manioc waste according to a specific proportion to be fermented, the bacillus subtilis BS-1 can be prepared into the buffalo silage. The antibacterial spectrum of the obtained silage is remarkably enhanced, and escherichia coli, campylobacter jejuni and salmonella causing diarrhea as well as streptococcus uberis, staphylococcus aureus and streptococcus agalactiae causing mastitis can be inhibited at the same time. In the feed, after 60%-75% of orange peel and 25%-40% of manioc waste are mixed, the crude protein content of a fermentation product is remarkably increased, and the fiber content is reduced. Feeding tests show that the feed can significantly improve the lactation yield and milk quality of the milk buffalo in the lactation period and effectively relieve diarrhea. The invention provides the silage which is safe and efficient and has nutrition and disease prevention functions.

Bacteroides fragilis and application thereof

Resumen de: CN122256201A

The invention relates to bacteroides fragilis and application thereof, the bacteroides fragilis is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 14, 2026, and the preservation number is CGMCC No. 38257. The strain can regulate and control STING-dependent training immunity, effectively resist infection of salmonella typhimurium and H9N2 avian influenza virus, maintain the integrity of a host intestinal barrier and relieve inflammatory injury. The bacteroides fragilis provided by the invention has good biological safety, can be used as a training immunity inducer, is expected to be used as a candidate probiotic or vaccine adjuvant for preventing/treating bacterial and virus infection, is suitable for human health and poultry breeding industry, and provides a new solution for resisting antibiotic resistance.

Broad-spectrum bacteriophage targeting mucous escherichia coli, cronobacter and salmonella and application of broad-spectrum bacteriophage

Resumen de: CN122256270A

The invention discloses a broad-spectrum bacteriophage targeting mucous escherichia coli, cronobacter and salmonella and application of the broad-spectrum bacteriophage. The invention provides a bacteriophage Escherichia coli phagevB EcoP 3BB7B capable of efficiently cracking mucus type enterobacter, the bacteriophage can specifically crack escherichia coli which can produce corac acid and is in a mucus type phenotype, and the technical bottleneck that adsorption of a mucus layer to most bacteriophages is limited is broken through; compared with the prior art, the recombinant plasmid has the advantages that the recombinant plasmid has high specific activity and cross-genus splitting capacity, not only has good splitting activity on mucus type escherichia coli, but also can effectively split important food-borne pathogenic bacteria such as various serotypes of salmonella, cronobacter sakazakii and the like, and the range of a host spectrum is remarkably widened. The bacteriophage can achieve efficient amplification under the condition of low infection complex number, is good in environmental stability, high in lysis activity, free of drug-resistant genes and toxicity-related genes and high in safety, and has good clinical and food safety prevention and control application prospects.

Gene chip for simultaneously detecting 12 food-borne pathogenic bacteria and application thereof

Resumen de: CN122235342A

The invention relates to the technical field of biological detection, and particularly discloses a gene chip for simultaneously detecting 12 food-borne pathogenic bacteria and application thereof, the gene chip comprises a polyvinyl chloride solid-phase carrier, and a specific probe, a positive control probe and a hybridization control probe which are fixed on the surface of the polyvinyl chloride solid-phase carrier. The gene chip can be used for simultaneously detecting salmonella, escherichia coli O157: H7, staphylococcus aureus, listeria monocytogenes, bacillus cereus, vibrio parahaemolyticus, vibrio cholerae, vibrio fluvialis, shigella flexneri, campylobacter jejuni, clostridium perfringens and yersinia enterocolitica. The gene chip disclosed by the invention is arranged by adopting a preset dot matrix, and detection is realized through sample total DNA extraction, multiple PCR amplification and biotin labeling, chip hybridization, washing, sealing, enzyme labeling, color development and scanning interpretation; the method can realize synchronous detection of 12 target bacteria, and has good specificity, repeatability and application value.

METHODS AND SYSTEMS FOR THE RAPID DETECTION OF LISTERIA USING INFECTIOUS AGENTS

Resumen de: AU2026204192A1

Disclosed herein are methods and systems for rapid detection of microorganisms such as Listeria spp. in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Listeria-specific bacteriophage, allows detection of a specific microorganism, such as Listeria spp. and an indicator signal may be amplified to optimize assay sensitivity. ay a y

- Recombinant Expression Vector for Secretion of Interferon- and Attenuated Salmonella Transformed Therewith

Resumen de: WO2024025312A1

The present invention relates to a recombinant expression vector for secretion of IFN-β proteins and an attenuated Salmonella strain transformed by means of same, and provides: a recombinant expression vector comprising flgM genes, IFN-β genes, and flhDC genes; an attenuated Salmonella strain transformed by means of same; and a pharmaceutical composition for cancer treatment comprising the attenuated Salmonella strain as an active ingredient.

一种Ulp1酶双抗夹心ELISA试剂盒及应用

Resumen de: CN120992924A

The invention belongs to the technical field of Ultrap1 enzyme quantitative detection, and particularly relates to an Ultrap1 enzyme double-antibody sandwich ELISA (enzyme-linked immuno sorbent assay) kit and application. Comprising an elisa plate coated with an anti-Ultrap1 enzyme mouse monoclonal antibody, an anti-Ultrap1 enzyme rabbit polyclonal antibody, an HRP-labeled goat anti-rabbit antibody, a diluent, a cleaning solution, a confining solution, a developing solution and a stop solution. According to the present invention, the complex of the Ulp1 enzyme and the SUMO tag protein is adopted as the immunogen to simultaneously immunize mice and New Zealand rabbits so as to prepare different species of the good affinity Ulp1 enzyme antibodies, and then the optimal reaction condition is further screened to develop the stable double-antibody sandwich ELISA detection kit with characteristics of high specificity and strong sensitivity, the defects in the prior art can be effectively overcome.

Application of salmonella bacteriophage vBSalDSP13 and rapid detection method of salmonella bacteriophage vBSalDSP13

Resumen de: CN122214285A

The invention discloses application of a salmonella bacteriophage vBSalDSP13 and a rapid detection method of the salmonella bacteriophage vBSalDSP13. The preservation number of the bacteriophage vBSalDSP13 is GDMCC No: 67876-B1, and the preservation number of the bacteriophage vBSalDSP13 The virulent bacteriophage vBSalDSP13 provided by the invention is wide in host spectrum, can split up to 25 salmonella serotypes, can split two strains of escherichia coli at the same time, and has a characteristic molecular target. The bacteriophage SP13 has good thermal stability, pH stability and chloroform tolerance, is not easy to inactivate in the application process, and has a good inhibition effect on target bacteria in milk and chicken. In conclusion, the bacteriophage SP13 can be used as a single inhibitor or combined with other pathogenic bacteria bacteriophages to form an inhibitor for preventing and controlling pathogenic bacteria such as salmonella and the like.

一种htrA基因敲除的减毒沙门氏菌重组工程菌株及其应用

Resumen de: CN122214226A

本发明公开了一种htrA基因敲除的减毒沙门氏菌重组工程菌株及其应用，对减毒沙门氏菌VNP20009进行基因组遗传改造，将基因组中的htrA基因敲除，获得ΔhtrA‑VNP菌株。通过回补性表达带有/不带有信号肽的HtrA蛋白，首次证实信号肽对于HtrA蛋白的功能发挥至关重要。无信号肽的htrA基因回补菌株与ΔhtrA‑VNP菌株生物学表型无显著差异。本发明所述的表达带有信号肽的HtrA蛋白的ΔhtrA‑VNP菌株，HtrA蛋白通过调控细菌胞外多糖水平，一方面影响菌株的免疫原性与抗癌效果，另一方面影响菌株对抗巨噬细胞的杀伤效果，进而影响细菌在体内的分布特性。本发明基于htrA基因活性作用模式，建立了瘤内时空特异性启动表达带有信号肽的HtrA蛋白的ΔhtrA‑VNP菌株，实现更高效、安全与精准的活体微生物抗肿瘤治疗。

Application of serine and glycine depletion engineering bacteria targeting soft tissue sarcoma

Resumen de: CN122214382A

The invention discloses an application of serine and glycine depletion engineering bacteria targeting soft tissue sarcoma, which comprises the steps of construction and identification of engineering bacteria SGS1, in-vitro experimental verification, material screening and preparation, and construction and identification, during use, screening and preparation of vector bacteria: selecting attenuated salmonella typhimurium VNP20009 as the vector bacteria, and screening and preparing the vector bacteria by using the attenuated salmonella typhimurium VNP20009 as the vector bacteria; the strain has high tumor enrichment, is purified by a centrifugal washing method after being subjected to sterile culture amplification, impurities and infectious microbes in a culture medium are removed for later use, a serine dehydratase gene from pseudomonas is selected, a target gene segment is obtained through a PCR amplification technology, and after the correctness of the segment size is verified through agarose gel electrophoresis, the target gene is recovered for later use. A secretory vector and a tumor microenvironment responsive promoter are selected, the secretory vector is subjected to enzyme digestion treatment, so that the vector has a site connected with a target gene, and meanwhile, the tumor microenvironment responsive promoter can specifically promote the expression of the target gene in a tumor microenvironment for later use.

Preparation of feed-grade nano zinc oxide with high specific surface area and application of feed-grade nano zinc oxide in piglets

Resumen de: CN122212224A

The invention discloses preparation of feed-grade high-specific-surface-area nano zinc oxide and application of the feed-grade high-specific-surface-area nano zinc oxide to piglets. Crude zinc oxide is adopted as a raw material, and the zinc oxide is prepared through the processes of ammonia leaching, ammonia distillation, calcination and the like. The feed-grade high-specific-surface-area nano zinc oxide prepared by the method has the specific surface area of more than 60m < 2 >/g, the ZnO content of more than 95%, the particle size of less than 100nm, the average particle size of 50-100nm, the content of harmful element lead of less than 10mg/kg, the arsenic content of less than 3mg/kg and the cadmium content of less than 8mg/kg. Compared with common nano-zinc oxide (OZO), the nano-zinc oxide has better stability in simulated piglet gastrointestinal fluid, has better bacteriostatic activity on escherichia coli K88, staphylococcus aureus and salmonella suis, has smaller toxicity on a pig small intestine epithelial cell line IPEC-J2, can significantly reduce cell apoptosis and generation of active oxygen in cells, and can be used for preparing the antibacterial nano-zinc oxide. 150mg/kg of the feed additive is added into daily ration of the first two weeks after weaning of the piglets to reduce the diarrhea of the weaned piglets, improve the average daily gain of the piglets and reduce the feed-gain ratio, and the feed additive has a good effect.

用于对饮食品样本、环境样本或生物体样本中的肠杆菌科细菌的有无和/或存在量进行检测的方法和试剂盒

Resumen de: EP4279503A1

0001 Provided is a method that enables the presence and/or amount of bacteria of Enterobacteriaceae in a food/drink sample, an environmental sample, or a biological sample to be detected easily and efficiently in a short amount of time. Said method includes a step for simultaneously detecting, on the basis of an antigen-antibody reaction, the presence and/or amount of bacteria of Enterobacteriaceae of at least two different genera in a sample.

WZ-93 strain for relieving constipation and application of WZ-93 strain

Nº publicación: CN122214196A 16/06/2026

Solicitante:

SHENZHEN DUNXING HEALTH TECH CO LTD
\u6DF1\u5733\u6566\u884C\u5065\u5EB7\u79D1\u6280\u6709\u9650\u516C\u53F8

Resumen de: CN122214196A

The invention relates to a strain of WZ-93 for relieving constipation and application thereof, the preservation number of the WZ-93 is CGMCC No.37033, and the WZ-93 has antibacterial activity on Escherichia coli, salmonella enteritidis and staphylococcus aureus, and can be used for treating constipation. The compound can be copolymerized with Escherichia coli, Salmonella enteritidis, Staphylococcus aureus, Streptococcus parablood, Enterobacter cloacae and Isostercas verbena, can grow and propagate by using oligosaccharide or D-psicose as a carbon source, can reduce secretion of inhibitory peptide neurotransmitters, can promote generation of excitatory peptide neurotransmitters, can promote secretion of anti-inflammatory factors, and can be used for preparing anti-inflammatory drugs. The composition can inhibit the secretion of proinflammatory factors, can enhance the intestinal peristalsis function, reduce the first black excrement discharge time, increase the excrement water content, improve the alpha-diversity of intestinal flora, promote the proliferation of beneficial bacteria and reduce the abundance of harmful bacteria, and can be used for preparing preparations or products for relieving constipation and preventing pathogenic bacterium infection.