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139
resultados
Última actualización
16/11/2025 [07:03:00]
Resumen de: CN120539398A
The invention belongs to the technical field of food microorganism detection, and provides a lateral flow chromatography kit for high-sensitivity quantitative detection of salmonella enteritidis, which comprises a salmonella enteritidis RPA primer group and a lateral flow chromatography test strip, raman label streptavidin, a Digoxin antibody and a streptavidin antibody are coated on the lateral flow chromatography test strip. The reporter probe is combined on the release pad of the flow measurement chromatography test strip, has two characteristics of colorimetry and SERS (Surface Enhanced Raman Scattering), can finish simultaneous qualitative and quantitative detection of pathogenic bacteria, and improves the detection sensitivity; and the Digoxin antibody and the streptavidin antibody are respectively combined on the detection line and the quality control line. The kit does not need to use large experimental equipment in the whole detection process, is high in detection accuracy and good in specificity, and is very suitable for real-time detection under the condition of relatively low development level.
Resumen de: CN120522382A
The invention discloses a nano-enzyme composite material for detecting pathogenic microorganisms in a complex matrix and application of the nano-enzyme composite material, and belongs to the technical field of pathogen detection. The kit containing the nano-enzyme composite material provided by the invention can realize accurate detection of food-borne pathogenic bacteria in a complex matrix, and has an excellent visual detection effect. And the environment-stable storage performance is excellent and can be up to 60 days. Excellent catalytic activity, stability and selectivity are shown in the aspect of detecting escherichia coli, staphylococcus aureus, vibrio parahaemolyticus and salmonella typhimurium. According to the invention, rapid and reliable detection can be realized, and the detection limit of different pathogens in a PB buffer solution (pH 7.2) is 2-7CFU/mL.
Resumen de: CN120518710A
The invention belongs to the technical field of biology, and particularly discloses an antibacterial peptide Meijia-1 and application thereof. The amino acid sequence of the antibacterial peptide is as follows: arginine, glycine, arginine, phenylalanine, glycine, arginine, phenylalanine, leucine, lysine, lysine, valine, arginine, arginine, phenylalanine, isoleucine, proline, lysine and valine. The antibacterial peptide Meijia-1 has better antibacterial activity on gram-positive bacteria (staphylococcus aureus) and gram-negative bacteria (escherichia coli and salmonella), and has the characteristics of heat resistance, salt resistance, acid and alkali resistance, repeated freezing and thawing resistance, serum resistance, organic solvent resistance and pepsin resistance. Therefore, the antibacterial peptide Meijia-1 has wide application prospects in the fields of pathogenic infection resistance, animal feed, food preservation and preservatives, cosmetics, detergents, disinfectants, pesticides, antibacterial molecular materials and the like.
Resumen de: CN120519328A
The invention belongs to the technical field of lead adsorption, and relates to lead-resistant lactobacillus salivarius Pb214 and application thereof. A new lead-resistant lactobacillus salivarius Pb214 is separated from cecum contents of healthy broilers, the classification name of the lead-resistant lactobacillus salivarius Pb214 is Lactobacillus salivarius, the preservation number of the lead-resistant lactobacillus salivarius Pb214 is CGMCC (China General Microbiological Culture Collection Center) NO.33992, and the lead-resistant lactobacillus salivarius Pb214 is preserved in China General Microbiological Culture Collection Center on March 27, 2025. Meanwhile, experiments prove that the maximum tolerance lead concentration of the lactobacillus salivarius is 1000mg/L, the average lead adsorption rate of the lactobacillus salivarius to a 5mg/L lead solution (pH 5) is 94.9%, the lead adsorption rate is the highest and reaches 96.33% when the pH is 7, and the lactobacillus salivarius can tolerate 0.3% cholate and also can inhibit the growth of salmonella pullorum and staphylococcus aureus. Therefore, the lead-resistant lactobacillus salivarius can be used for removing the lead metal element in a matrix, and the matrix can be soil, feed, a culture medium and other substances needing lead removal.
Resumen de: WO2024155027A1
The present invention relates to an attenuated Salmonella gallinarum expressing FliC or FliC-hiL2 and use thereof. The Salmonella strain according to the present invention has excellent immune activity and exhibits excellent anti-cancer efficacy, and thus can be used as a therapeutic agent for cancer together with or independently of existing anti-cancer drugs.
Resumen de: CN120514781A
The invention relates to the technical field of application of natural active substances, in particular to application of tea polyphenol in yellow tea in inhibition of bacteria, gram-positive bacteria comprise staphylococcus aureus, bacillus subtilis, enterococcus faecalis and enterococcus faecium. The gram-negative bacteria comprise escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, proteus mirabilis, salmonella and acinetobacter baumannii. The bacteriostatic activity and related mechanism of the tea polyphenol in the yellow tea are systematically studied, the bacteriostatic effect of the tea polyphenol in the yellow tea on gram-negative bacteria and gram-negative bacteria is proved, and the bacteriostatic mechanism of the tea polyphenol in the yellow tea on bacteria is disclosed through bacteriostatic experiments of the tea polyphenol on different bacteria, so that the bacteriostatic effect of the tea polyphenol in the yellow tea on the gram-negative bacteria and the bacteriostatic effect of the tea polyphenol in the yellow tea on the gram-negative bacteria are proved. The bacteriostatic activity and related mechanisms of the tea polyphenol in the yellow tea are systematically researched, the development of potential clinical medicinal value and edible value of the tea polyphenol is facilitated, and a foundation is laid for the application of the tea polyphenol in the fields of food, medicine and the like.
Resumen de: CN120518775A
The invention relates to the technical field of biology, in particular to fusion protein, a construction method and application. A new fusion gene is constructed by adopting an SOE-PCR technology for the first time, the fusion protein H3-OtTx1a is obtained by expression in a suitable expression system, the functions of parent proteins of the fusion protein H3-OtTx1a are integrated, the antibacterial activity of the fusion protein H3-OtTx1a is higher than that of hermetia illucens antibacterial peptide H3 and spider peptide OtTx1a, and the fusion protein H3-OtTx1a has good thermal stability, acid-base stability and endogenous protease degradation resistance, and can be used for preparing the antibacterial peptide H3 and spider peptide OtTx1a. Growth of enterobacter, salmonella and staphylococcus aureus can be obviously inhibited, and the compound has wide application prospects in the fields of food, hygienic products, cosmetics, biopesticides, biological feed additives or natural food preservatives and the like.
Resumen de: CN120514728A
The invention discloses application of synanthrin in improving the action efficiency of ampicillin on drug-resistant salmonella, and relates to the technical field of medical biology. Researches show that synanthrin does not have an inhibiting effect on salmonella, but synanthrin and ampicillin are used together, the inhibiting effect of ampicillin on salmonella can be remarkably improved, and same conclusions are drawn by in-vitro tests and in-vivo tests. According to the invention, the use of antibiotics can be reduced, and a new research direction is provided for the prevention and treatment of salmonella.
Resumen de: CN120522381A
The invention provides a kit for detecting shigella by a Marker method, and the Marker method represents fluorescence intensity corresponding to shigella with different concentrations and is a basis for establishing a Marker graph; the kit comprises a Marker graph, a magnetic aptamer, a detection aptamer, a bacterial lysis solution, an enzyme digestion solution, a binding solution, a washing solution and a magnetic frame. The detection method comprises the following steps: the kit is used for splitting the shigella through a bacterial lysis solution to release DNA, the DNA is cut through an enzyme digestion solution to obtain a fragment rich in an ipaH gene, the magnetic aptamer and the detection aptamer are specifically combined with the ipaH fragment under the action of a binding solution to form a compound, the compound is washed with washing liquid, the fluorescence intensity is detected and compared with a Marker diagram, and quantitative detection of the shigella is realized. The method does not need to treat a sample in advance, shortens the detection time, and has strong specificity and high sensitivity.
Resumen de: WO2025175111A1
Two stage heated water treatment of shell eggs results in-shell poached eggs. The in-shell poached are sufficiently cooked so when the eggs are cracked onto a plate the eggs have the characteristics of conventional poached eggs without further cooking and need only to be warmed for consumption. The in-shell poached eggs are also pasteurized in the shell to meet the FDA and WHO requirement of a 5-log reduction of Salmonella Enteritidis.
Resumen de: WO2025171792A1
Provided is use of a combination of three or more of the following microorganisms in the preparation of an anti-tumor combination vaccine: Bordetella pertussis, Salmonella typhi, Salmonella paratyphi A, Salmonella paratyphi B, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, Proteusbacillus vulgaris, Lactic acid bacteria, Bifidobacterium longum, Bordetella pertussis, Corynebacterium diphtheriae and Clostridium tetani, Clostridium acetobutylicum, Salmonella typhimurium, and Streptococcus pyogenes. The anti-tumor combination vaccine is broad-spectrum, safe and non-toxic, and thus has the prospect of clinical applications.
Resumen de: US2025261659A1
Two stage heated water treatment of shell eggs results in-shell poached eggs. The in-shell poached are sufficiently cooked so when the eggs are cracked onto a plate the eggs have the characteristics of conventional poached eggs without further cooking and need only to be warmed for consumption. The in-shell poached eggs are also pasteurized in the shell to meet the FDA and WHO requirement of a 5-log reduction of Salmonella Enteritidis.
Resumen de: KR20240015036A
The present invention relates to a recombinant expression vector for secretion of ifn-03b2# protein and an attenuated salmonella strain transformed by the same. provided are a recombinant expression vector comprising a flgm gene, an ifn-03b2# gene, and a flhdc gene, an attenuated salmonella strain transformed by the same, and a pharmaceutical composition for treating cancer comprising the attenuated salmonella strain as an active ingredient.
Resumen de: KR20240015036A
The present invention relates to a recombinant expression vector for secretion of ifn-03b2# protein and an attenuated salmonella strain transformed by the same. provided are a recombinant expression vector comprising a flgm gene, an ifn-03b2# gene, and a flhdc gene, an attenuated salmonella strain transformed by the same, and a pharmaceutical composition for treating cancer comprising the attenuated salmonella strain as an active ingredient.
Resumen de: CN120051482A
The present invention relates to a modified live attenuated Gram-negative bacterium wherein the bacterium has been modified such that an RNA molecule can be safely and efficiently delivered to a target eukaryotic cell. Thus, the present invention relates to a bacterial delivery system and various uses and methods thereof.
Resumen de: CN120505437A
The invention relates to the technical field of salmonella detection, in particular to a salmonella molecular detection method and application thereof, and the salmonella molecular detection method comprises the following steps: S1, sample treatment: extracting DNA in a to-be-detected sample; step S2, primer design and synthesis: specific primers are designed for the invA gene, the hilA gene and the stn gene of salmonella; s3, multiple PCR reaction: taking the DNA extracted in the step S1 as a template, and carrying out multiple PCR amplification by adopting the primer in the step S2; s4, fluorescent probe hybridization: hybridizing a PCR amplification product in the step S3 with a fluorescent labeled probe; s5, fluorescence signal detection: detecting a fluorescence signal after hybridization through a fluorescence detector, and judging whether the sample contains salmonella or not according to the signal. According to the salmonella molecular detection method provided by the invention, a technology of combining multiple PCR with fluorescent probe hybridization is adopted, a plurality of conserved genes of salmonella can be detected at the same time, and the detection specificity and sensitivity are improved.
Resumen de: WO2025170108A1
The present invention relates to identifying and engineering a tail spike protein gene from a genome of bacteriophage SFP10 infected with a Salmonella strain, purifying and producing a recombinant protein using mSFP10TSP, which is a recombinant protein formulation for detecting Salmonella enterica, EGFP-mSFP10TSP fused with a green fluorescent protein, and SiBD-mSFP10TSP fused with an SiBD, and thereby specifically detecting the Salmonella strain.
Resumen de: CN120505321A
The invention relates to the technical field of salmonella detection, in particular to a multivalent aptamer probe for capturing salmonella, a preparation method of the multivalent aptamer probe and application of the multivalent aptamer probe to salmonella detection. The invention relates to a multivalent aptamer probe for capturing salmonella. The multivalent aptamer probe is prepared from a DNA sample H1, a DNA sample H2, an activation chain, an aptamer and cDNA dry powder through a hybridization chain reaction technology. The multivalent aptamer probe is used in the salmonella detection process, and has the advantages of one-tube detection, no secondary pollution and high detection sensitivity.
Resumen de: CN120505438A
The invention discloses a composition and method for detecting food-borne pathogenic bacteria and application, and belongs to the technical field of food safety detection. The gDNAs and fluorescent probe combination suitable for multi-target parallel detection is obtained through multiple rounds of screening, meanwhile, the advantages of LAMP efficient amplification and PfAgo targeted cutting are combined, a multiple LAMP-PfAgo rapid detection method for three pathogenic bacteria of staphylococcus aureus, salmonella and listeria monocytogenes is established, and the problem that LAMP multiple detection is difficult is effectively solved. According to the present invention, the specificity is high, the result determination is simple, the detection limit on the pure culture can achieve 101 CFU/mL, the detection can be completed within 45 min, and the important practical application value is provided.
Resumen de: CN120504728A
The invention discloses an application of a polypeptide in inhibiting bacteria or preparing a reagent for inhibiting bacteria, and belongs to the technical field of biology. According to preferred codons of bacillus subtilis, a polypeptide coding gene which is derived from myxobacteria and has unknown functions is designed, and a nucleotide sequence is obtained. And then carrying out recombinant expression in bacillus subtilis by utilizing a genetic engineering technology. The fermentation liquor of the recombinant bacillus subtilis shows better antibacterial activity on gram-positive bacteria represented by staphylococcus aureus, pseudomonas aeruginosa, salmonella, escherichia coli and gram-negative bacteria represented by vibrio parahaemolyticus. The antibacterial function of the polypeptide in myxobacteria is found for the first time, namely, the novel antibacterial peptide is provided, and compared with traditional antibiotics, the novel antibacterial peptide is equivalent or higher in antibacterial activity, wide in antibacterial spectrum and low in preparation cost and has wide application prospects in the fields of antibacterial preparations, food preservatives and the like.
Nº publicación: CN120505281A 19/08/2025
Solicitante:
HARBIN VETERINARY RES INSTITUTE CAAS HARBIN BRANCH OF CHINA ANIMAL HEALTH AND EPIDEMIOLOGY CENTER
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Resumen de: CN120505281A
The invention discloses a hybridoma cell strain secreting an anti-salmonella abortus groEL protein monoclonal antibody, the monoclonal antibody and application of the hybridoma cell strain. The hybridoma cell strain is named as E11, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the strain preservation number of the hybridoma cell strain is CGMCC No.46349. According to the present invention, the salmonella universal cELISA antibody detection method capable of being applied to different animals is established by using the E11 MAb secreted by the hybridoma cell strain secreting the anti-horse salmonella abortus groEL protein monoclonal antibody and the horse salmonella abortus groEL protein; the salmonella comprises salmonella abortus (S.Abortuqui), salmonella typhimurium (S.Typhi), salmonella dublin (S.Dublin) and salmonella enteritidis (S.Enteridis), so that the method disclosed by the invention has the advantages of good specificity and broad spectrum. The invention provides an effective technical means for diagnosis, prevention and control of salmonellosis of different animals clinically.
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