Absstract of: CN119752763A
The invention relates to application of carbon nitride in microalgae culture and a method for culturing microalgae, which comprises the step of adding carbon nitride into a culture system. According to the method, the graphite phase carbon nitride (g-C3N4) material is added into a microalgae culture system to obtain the g-C3N4-microalgae hybrid, so that the growth speed of microalgae and the accumulation speed of biomass are increased, and the yield of target metabolites in microalgae can also be increased. The practice of the invention creates a new path for breaking through the inherent bottleneck of the natural photosynthetic system of cyanobacteria and improving the photosynthetic carbon sequestration efficiency.
Absstract of: CN119746618A
The invention provides a regeneration gas recycling system, which comprises a regeneration gas separator, a regeneration tower, a regeneration tower, a regeneration tower and a regeneration tower, an inlet of the water collecting tank is connected with a liquid outlet of the regenerated gas separator; an inlet of the gas storage tank is connected with a gas outlet of the regenerated gas separator; the microalgae culture tank is used for culturing microalgae; a nutrient solution is stored in the nutrient solution circulating assembly, an outlet of the moisture collecting tank is connected with an inlet of the nutrient solution circulating assembly, and an outlet of the nutrient solution circulating assembly is connected with a liquid inlet of the microalgae culture tank; an outlet of the gas storage tank is connected with an inlet of the carbon dioxide injection assembly, and an outlet of the carbon dioxide injection assembly is connected with a carbon dioxide inlet of the microalgae culture tank, so that the problem that in the carbon capture process in the prior art, water and carbon dioxide in treated exhaust gas are generally discharged together is solved.
Absstract of: CN119751129A
The invention belongs to the technical field of organic fertilizers, and particularly relates to a composite algae cell as well as a preparation method and application thereof. According to the preparation method disclosed by the invention, the stable algae cell with the nanoparticle layer is obtained by co-incubation of the positively charged amino-modified nanoparticles and the algae cell; and mixing and emulsifying a tannic acid aqueous solution and the algae cells with the nano-particle layers to obtain nano-particle algae cells containing lipid membrane layers, culturing under illumination, and carrying out photosynthesis to obtain the composite algae cells with the inner cores carrying oxygen. In addition, the lipid membrane can carry pesticide and other substances, and the antibacterial ability of the composite algae cells obtained by the method is greatly improved; fertilization is conducted in an ultrasonic atomization delivery mode, the mass transfer efficiency of microalgae fertilizer to leaf surfaces is improved, and the fertilizer utilization rate is remarkably increased.
Absstract of: CN119750795A
The invention discloses an efficient microalgae resourceful treatment method which comprises the following steps: inoculating a microalgae concentrated solution with the dry weight of more than 30mg/L into wastewater to be treated, then respectively adding nano zero-valent iron suspensions with different concentrations at different physiological stages of microalgae, culturing for 14 days to obtain a microalgae culture solution, detecting the indexes of COD, NH4 < + >-N and PO4 < 3->-P in the microalgae culture solution, and determining the content of the microalgae in the wastewater to be treated according to the indexes of COD, NH4 < + >-N and PO4 < 3->-P in the microalgae culture solution. The method comprises the following steps: standing a microalgae culture solution for 9 hours, carrying out self-flocculation settling on microalgae, carrying out centrifugal treatment on the microalgae culture solution subjected to self-flocculation settling, filtering, carrying out freeze drying to obtain microalgae powder, and extracting the microalgae grease by adopting a chloroform-methanol method. According to the method, nano zero-valent iron with different concentrations is added in different growth stages of the microalgae, so that the performance of removing nutrient substances in the wastewater by the microalgae is enhanced, and meanwhile, the flocculation capacity of the microalgae and the accumulation of grease content are promoted.
Absstract of: CN119751617A
The invention provides an antibacterial peptide and application thereof in juvenile mollusk culture. The amino acid sequence of the provided antibacterial peptide is SEQ ID NO: 2. The antibacterial peptide obtained through screening comes from bivalve shellfish, has small toxic and side effects on the bivalve shellfish, is suitable for playing a role in the bivalve shellfish, and has specificity and pertinence on bivalve shellfish culture water pathogenic bacteria. The amino acid sequence of the provided antibacterial peptide is optimized, the antibacterial activity is high, the stability is high, and the expression efficiency in yeast is high. After high expression of the antibacterial peptide, the microcapsule of which the particle size is suitable for filter feeding of the juvenile mollusks is prepared, so that the problem that the juvenile mollusks cannot feed due to direct splashing to culture water in the prior art is solved, and the problems of low microalgae expression efficiency and low antibacterial peptide content are solved.
Absstract of: CN119752635A
The invention discloses a method and an expression system for expressing bacteriophage lyase by using chlorella vulgaris. The microalgae vector takes chlorella vulgaris as an expression vector, and recombinant plasmids are electrically transferred into cell nucleus genes of the chlorella vulgaris. The method is simple, safe and low in cost. The carrier is high in microalgae system conversion rate, and the expressed lyase has good activity to bacteria. The chlorella vulgaris is an edible alga, and has a wide prospect in the subsequent application of oral administration or perfusion. Meanwhile, the fluorescence imaging performance of the microalgae system is outstanding, and possibility is provided for non-invasive tracking and real-time monitoring of the lyase in vivo. The unique characteristics of chlorella vulgaris are utilized, and a feasible thought and method are provided for efficient expression of the lyase.
Absstract of: CN119752634A
The invention discloses a culture method of microalgae with high starch content, and belongs to the technical field of new food resource mining. Microalgae are cultured in two stages, the ventilation capacity is fixed in the first stage, the ventilation capacity is dynamically adjusted in the second stage, the ventilation capacity in the early stage of culture is 0.75-1.5 L/min, the ventilation capacity is adjusted to 3-6 L/min when the microalgae start to grow in the exponential stage, and the ventilation capacity is adjusted to 0.75-1.5 L/min when the microalgae start to grow in the later stage of the exponential stage until culture is finished. The high-efficiency culture of the microalgae with high starch content is realized by dynamically adjusting the ventilatory capacity of the second culture stage, so that the microalgae cell powder with high starch content is obtained, and the maximum starch content can reach 61% or above. The method has the advantages of short required culture period, simplicity and convenience, no pollution to the environment in the culture process, and important significance for expanding the microalgae as a new resource food and expanding a new starch resource.
Absstract of: CN119752633A
The invention discloses a method for promoting expression of a chlamydomonas reinhardtii selenoprotein gene and increasing the yield of selenoprotein. The method comprises the following steps: S1, inoculating a chlamydomonas reinhardtii algal species into a liquid culture medium, and carrying out shake culture under the illumination conditions of 20-30 DEG C and 0-200 mu mol/(m < 2 >. S) until a growth delay period to a stable period to obtain a chlamydomonas reinhardtii culture solution; s2, the chlamydomonas reinhardtii culture solution is taken, sodium selenite and abscisic acid are added, the concentration of the abscisic acid in the culture solution is 10-1000 mu mol/L, and shaking culture is conducted under the illumination conditions of 20-30 DEG C and 0-200 mu mol/(m < 2 >. S). The plant hormone ABA is added in the chlamydomonas reinhardtii selenium-rich culture process, microalgae growth and biomass accumulation are not affected, expression of selenoprotein can be promoted, and when the method is applied to production of selenoprotein-rich chlamydomonas reinhardtii powder, the selenoprotein yield can be greatly increased.
Absstract of: US2025109414A1
Apparatus 101 for producing a continuous stream of biohydrogen includes a continuous flow stirred tank reactor (CSTR) 12, lighting arrangement 13; ports 14; peristaltic pumps 16; and gas chromatograph (GC) 18. The CSTR 12 includes internal bioreactor chamber 20; a photosynthesizing microorganism inlet stream 22.1 for delivering Chlorella vulgaris; an organic waste inlet stream 22.2 for delivering wastewater activated sludge (WWAS); and a nutrient inlet stream 22.3 for delivering nutrients into the chamber 20; an outlet stream 24; and stirrers for stirring the contents of the chamber. The lighting 13 is provided by lamps 26 for transmitting light through a transparent sidewall of the chamber 20. The ports 14 and CG 18 are for taking measurements from the chamber 20. The pumps 16 control flowrate of the streams 22.1, 22.2, 22.3 and 24 for regulating biohydrogen production from fermentation while Chlorella vulgaris consumes oxygen to avoid biohydrogen conversion into methane.
Absstract of: US2025108080A1
Provided are compositions containing extracellular vesicles (MEVs) produced in and isolated from genetically-engineered microalgae, which encode cargo, such as nucleic acids and polypeptides. The MEVs are endogenously loaded by the microalgae with the cargo. The MEVs have a variety of applications as a delivery system for therapeutics, including vaccines, anti-cancer therapeutics, diagnostics, and other such uses. The fate of MEVs upon administration depends upon the route of administration.
Absstract of: AU2023350703A1
The present invention relates to a method for harvesting microalgae, the method comprising (i) mixing one or more monomers, a photoinitiator, water and a deoxygenation system to form a mixture; (ii) exposing the mixture to UV light so as to form an aqueous solution of one or more polymers; (iii) introducing the aqueous solution of one or more polymers to a suspension comprising microalgae in an amount sufficient to produce flocculated microalgae; and (iv) harvesting the flocculated microalgae. The present invention also relates to a method for preparing and selecting a polymeric flocculant for use in harvesting microalgae and a related apparatus.
Absstract of: WO2025070726A1
The purpose of the present invention is to provide a preparation that is effective for maintaining or increasing a hair fibrous structural protein. Provided are a composition for maintaining or increasing a fibrous structural protein, a composition for maintaining or increasing skin elasticity, a composition for improving hair quality, and a composition for promoting the production of collagen, elastin, or hyaluronic acid, wherein each of the compositions contains a culture supernatant of microalgae and/or extracellular vesicles of microalgae.
Absstract of: MX2025000775A
A system and method for growing microalgae capable of mixotrophic metabolism, preferably Chlorella sp. Microalgae grown in the system using the method are able to survive and grow in dark refrigeration, which allows the algae to be stored and transported for application as a live culture. In addition, the microalgae can be grown in sufficient quantities to be sold commercially for application to crops as a biostimulant.
Absstract of: CN119736165A
According to the high-concentration microalgae fresh-keeping method based on the gel, sodium alginate and agar are dissolved to form hydrogel, then microalgae cells and carrageenan are added for 3D printing and cross-linking solidification, the printable solid content of the sodium alginate gel is reduced through the microalgae hydrogel formula, the activity and biomass of microalgae are effectively preserved, and the microalgae fresh-keeping effect is improved. The microalgae breeding and transportation are further ensured not to be influenced by seasons.
Absstract of: CN119736144A
The invention relates to an intelligent regulation and control ceramic membrane microalgae recovery system and method. The invention relates to the technical field of microalgae recovery, which comprises the following steps: inoculating a target microalgae variety in a microalgae culture pond, and setting proper initial culture conditions such as temperature, illumination intensity and nutrient substance addition amount according to the growth characteristics of microalgae; various parameters in the microalgae culture pond are monitored in real time through the intelligent monitoring module; according to monitoring data, the ceramic membrane filtering unit is controlled through the feedback control unit, the filtering performance of a culture solution is improved, and the stability of membrane flux is maintained. Compared with a traditional ceramic membrane recovery method, the method has the advantages that the microalgae recovery efficiency is remarkably improved by 30% or above, the membrane pollution degree is reduced by 40% or above, the economic benefits and environmental benefits of microalgae recovery are greatly improved, and a solid foundation is laid for large-scale industrial development and utilization of microalgae resources.
Absstract of: CN119736227A
The invention relates to application of high-temperature-resistant and high-light cyanobacteria in preparation of a biological photovoltaic device, the high-temperature-resistant and high-light cyanobacteria is synechococcus PCC 7942 obtained by introducing C252Y mutation on an ATP synthase alpha subunit, and the reference sequence of the ATP synthase alpha subunit is shown as SEQ ID NO: 1. According to the invention, a high-tolerance mutant strain HL7942 is used as a photoelectric conversion core element, so that a biological membrane cathode is formed on a substrate, a biological photovoltaic device can stably carry out light energy conversion output under the condition of high temperature and high light (2400 mu mol photons m <-2 > s <-1 >, 35 DEG C), and the light current output level is increased by 2.54 times compared with that of an early-stage device. Chitosan is used for packaging a biological membrane electrode, so that a biological membrane is stably attached to a substrate while the photosynthetic activity is maintained, the photocurrent output density of the device is improved, and the stable output period of the device is prolonged.
Absstract of: CN222700286U
The utility model relates to the technical field of spirulina cultivation, in particular to small balcony microalgae cultivation equipment. The barrel type bioreactor is used as a spirulina culture container and has the advantages of being large in volume, convenient to clean, simple to install, low in manufacturing cost and the like, only an air blower is used for aeration in the spirulina growth process, the spirulina growth is not affected, meanwhile, a multi-barrel parallel connection mode is adopted, the yield of fresh spirulina is increased, and the cost is reduced. The fresh food requirements of small families can be basically met. The device comprises an intelligent electric cabinet, a special light supplementing lamp, a bioreactor and the like, the output end of the intelligent electric control box is connected with five special light supplementing lamps, and a bioreactor is arranged between every two special light supplementing lamps. According to the utility model, the barrel-type bioreactor is used as a spirulina culture container, only an air blower is used for aeration in the spirulina growth process, and meanwhile, a multi-barrel parallel connection mode is adopted, so that the total capacity of the bioreactor reaches 100-120L, and 60-80g of fresh algae can be produced every day.
Absstract of: CN119709359A
The invention discloses an agricultural microalgae fertilizer in-situ manufacturing and applying device and method, and belongs to the field of microbial fertilizer manufacturing, the device comprises a first box body, the first box body is filled with a culture solution and microalgae, the first box body is communicated with a supplement pipe, the side wall of the first box body is provided with a plurality of overflow small holes, and the overflow small holes are communicated with the supplement pipe; the supplementing pipe can supplement a culture solution into the first box body, the supplementing pipe injects the culture solution into the first box body, and when the liquid level of the culture solution reaches the overflow small hole, the culture solution containing microalgae flows out to the soil, and when injection of the culture solution into the first box body is stopped, the microalgae remaining in the first box body continues to grow; the device is easy and convenient to operate, efficient preparation and application of the microalgae fertilizer can be achieved on the premise that the activity of microalgae cells is not damaged, the manufacturing and transportation cost of the microalgae fertilizer can be greatly reduced, the utilization efficiency of the fertilizer can be improved, and healthy growth of crops can be promoted.
Absstract of: CN119707120A
The invention discloses a temperature-alternating microalgae membrane reactor high-efficiency nitrogen and phosphorus removal method, which comprises a reactor main body, a ceramic membrane assembly is arranged in the reactor, and microalgae are inoculated in the reactor; the reactor main body is internally provided with a stirring device for uniformly suspending microalgae in the reactor; the reactor main body is made of a transparent material, and a light source is arranged outside the reactor main body; the top of the reactor main body is communicated with a water inlet pipeline for water feeding, and the liquid level is controlled by a liquid level controller; the top of the ceramic membrane component is communicated with a water outlet pipeline; a water outlet pump is arranged on the water outlet pipeline; a temperature control device is arranged on the outer side of the reactor and can monitor, regulate and control the temperature of liquid in the reactor. According to the method, the microalgae are subjected to suspension culture in the microalgae membrane reactor in a temperature alternating manner, the sensitivity of the microalgae to the environment temperature is fully utilized, the method has the advantages of low energy consumption, few byproducts and the like, and the synchronous nitrogen and phosphorus removal efficiency of the microalgae can be remarkably improved.
Absstract of: CN119731197A
The present invention relates to engineered single-cell native algae bacteria microalgae comprising a loss of homologous Seipin gene function, an in vitro process for the production of triacylglycerol (TAG), and uses thereof.
Absstract of: CN119709410A
The invention relates to a desert algae sand burying preservation method. The desert microalgae are inoculated into the sandy soil culture medium obtained by mixing the algae culture medium and the sandy soil, so that desert algae cells are preserved in the culture medium close to the original living environment, the preservation time of the desert algae cells can be effectively prolonged, the survival rate can be increased, and long-term stable preservation of the desert algae cells with specific forms and physiological characteristics can be ensured. The method is simple and rapid to operate, low in application cost and capable of keeping the original hereditary characteristics of algae species for a long time and improving the preservation effect.
Absstract of: CN222684635U
The utility model discloses an integrated device based on microalgae culture harvesting and water circulation, which comprises a microalgae culture tank, a microalgae temporary storage tank and a microalgae harvesting tank, the microalgae culture tank is fixedly arranged below the microalgae temporary storage tank, the microalgae harvesting tank is fixedly arranged on one side of the microalgae culture tank, and the microalgae harvesting tank is fixedly arranged on the other side of the microalgae culture tank. An air inlet pipe and a liquid inlet pipe are respectively mounted at the top of the microalgae temporary storage tank, a culture conveying pipe is fixedly connected between the microalgae culture tank and the microalgae temporary storage tank, a valve C is mounted on the culture conveying pipe, a harvesting conveying pipe is fixedly connected between the microalgae temporary storage tank and the microalgae harvesting tank, and a valve A is mounted on the harvesting conveying pipe. According to the microalgae culture and harvesting integrated device, microalgae culture and harvesting are integrated, the structure is simplified, the construction cost is reduced, the operation difficulty is reduced, the possibility that microalgae are polluted is reduced, and commercial production of microalgae is met.
Absstract of: CN119709361A
The invention discloses a closed microalgae culture treatment device for pollutant treatment and carbon sequestration, and relates to the technical field of pollutant treatment.The closed microalgae culture treatment device is characterized in that a closed pipeline culture device in the prior art is modified, and adherent microalgae are cleaned by arranging a cylindrical sliding block in a tubular culture treatment unit; a plurality of annular air bags with adjustable expansion and contraction are arranged on the side wall of the cylindrical sliding block; an independent pipe body is arranged to be responsible for conveying a culture solution into the tubular culture treatment unit and outputting the culture solution from the tubular culture treatment unit; a channel for a culture solution to flow is formed in the cylindrical sliding block, and the culture solution is efficiently stirred in the moving process of the cylindrical sliding block; the closed microalgae culture treatment device for pollutant treatment and carbon sequestration has the technical effects that the cleaning strength of the closed microalgae culture treatment device for the pollutant treatment and the carbon sequestration is adjustable, the cleaning effect is stable, the microalgae culture solution is not easy to pollute during transfer, and the stirring efficiency of the microalgae solution is high.
Absstract of: CN119709367A
The invention provides an algae-light complementary microalgae culture system, which comprises a photovoltaic cell module, the photovoltaic cell module comprises an electric sunshade net, a storage battery and a plurality of groups of solar photovoltaic panels, and the electric sunshade net and the plurality of groups of solar photovoltaic panels are electrically connected with the storage battery; the sewage storage and transportation module is located under the electric sunshade net and electrically connected with the storage battery, and the sewage storage and transportation module is used for sewage storage, sterilization and temperature control; the microalgae culture module is located under the electric sunshade net and connected with the sewage storage and transportation module, and the microalgae culture module is used for culturing target microalgae; the remote control module is electrically connected with the electric sunshade net, the sewage storage and transportation module and the microalgae culture module. The problems that a photovoltaic power generation technology mentioned in the background technology is low in space utilization efficiency, and smoke CO2 and waste water generated by a biomass power plant are difficult to treat are solved.
Nº publicación: CN119709617A 28/03/2025
Applicant:
BEIJING INSTITUTE OF TECH
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Absstract of: CN119709617A
The invention provides an application of a Synecocus elongatus PCC 7942, and relates to the technical field of biomedicine, in particular to an application of the Synecocus elongatus PCC 7942. According to the present invention, the Synchrococcus elongatus PCC 7942 belongs to cyanobacteria, and the research results of the inventor show that the strain has functions of I-type macrophage differentiation promotion, fibroblast activation inhibition and PD-L1 expression inhibition, can remodel the tumor microenvironment immunosuppression state, has good safety, and has good application prospects in tumor treatment. The invention also provides a method for inducing the Synecocus elongatus PCC 7942 to kill cancer cells, the method is simple and efficient, and the killing ability of the strain on cancer cells can be obviously improved only by performing ultrasonic treatment on the strain.