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ALGAE-BASED BIOPLASTICS AND METHODS OF MAKING

Resumen de: US2025145938A1

Provided for are methods of producing triacylglycerol-accumulated microalgae, methods for making bioplastics from triacylglycerol-accumulated microalgae, methods for making alga-mixed plastics, and products including these bioplastics. Methods of triacylglycerol accumulation using centrifugation are also provided. Products such as plastic beads and other consumer products can be made from the bioplastics described herein.

Oral broad-spectrum vibrio subunit microalgae vaccine for aquatic products and preparation method thereof

Resumen de: CN119925585A

The invention belongs to the field of microorganisms, and particularly relates to an oral broad-spectrum vibrio subunit microalgae vaccine for aquatic products and a preparation method of the oral broad-spectrum vibrio subunit microalgae vaccine. The invention provides an aquatic oral broad-spectrum vibrio subunit microalgae vaccine which expresses vibrio membrane protein OmpK by taking microalgae as a vaccine carrier. The gene expression cassette of the vibrio membrane protein OmpK is inserted into a genome of microalgae, and the sequence of the gene expression cassette of the vibrio membrane protein OmpK is as shown in SEQ ID NO: 4 after sequence optimization. When the microalgae vaccine is used for feeding cultured animals such as fishes and shrimps, the vibrio resistance can be remarkably improved, the environmental safety is high, and the large-scale production cost is low.

Sample collection container for liquid microalgae space technology test

Resumen de: CN119931821A

The invention discloses a sample collection container for a liquid microalgae space technology test. The sample collection container comprises a vibration isolation soft bag, a heat preservation module and a sample collection container, the vibration isolation soft bag is composed of an upper cover, a bag body and a zipper, a vibration isolation body made of polymer foam materials is arranged in the upper cover and used for relieving the influence of impact, overload, vibration and other environments on the heat preservation module and the sample collection container in the rocket launching and returning capsule returning period, and internal objects are protected to be smoothly injected into a rail and returned. The heat preservation module is composed of a shell, an inner cover and an inner container, and the inner container is filled with phase change cold storage materials. And the temperature control effect is achieved. And a suitable environment is provided for the liquid-based microalgae needing low-temperature descending. The sample collection container is composed of a bottle body, a one-way conducting valve, a diaphragm, a connector, a protective cap, a bottle cap and a guide pipe, and sample collection under the weightless environment is achieved. According to the invention, through vibration isolation design, air pressure balance design and one-way sealing design, a sample collection container and package for reliable liquid algae sample collection and protection

Application of P450 monooxygenase in increasing content of fucoxanthin in microalgae

Resumen de: CN119932129A

The invention belongs to the technical field of biology, and discloses application of P450 monooxygenase in increasing the content of fucoxanthin in microalgae, the microalgae with the fucoxanthin synthesis and/or light stress response capacity improved and a construction method of the microalgae. Researches find that P450 monooxygenase is closely related to synthesis of fucoxanthin and plays a regulating role in light stress response of diatom, and overexpression of P450 monooxygenase can cause increase of the content of fucoxanthin in microalgae; under the condition of light stress, the content of fucoxanthin in a transformant is greatly increased compared with that of wild type fucoxanthin. According to the invention, an engineering algal strain with improved fucoxanthin synthesis and/or light stress response capability is also obtained, a material basis is provided for the biosynthesis industrialization of fucoxanthin, and the additional value of microalgae culture is improved at the same time.

Sewage treatment device and sewage treatment method

Resumen de: CN119930098A

The invention relates to the technical field of sewage treatment, and discloses a sewage treatment device and a sewage treatment method.The sewage treatment device comprises a container, a diaphragm, a light source and a backflow pipe, a containing cavity is formed in the container, the diaphragm is arranged in the container and divides the containing cavity into a first area and a second area, and the diaphragm is suitable for small molecules and soluble solids to pass through; the light source is arranged on one side of the second area, the backflow pipe is communicated with the first area and the second area, the interior of the container is divided into two spaces through the diaphragm, sludge can only react in the first area, microalgae can only conduct photosynthesis in the second area, and the microalgae subjected to photosynthesis flows back into the first area through the backflow pipe, so that the microalgae can be recycled, and the yield of the microalgae is improved. The sludge activity can be improved, the sewage treatment aeration energy consumption can be reduced, the sewage treatment nitrogen and phosphorus removal effect can be improved, and CO2 emission in the sewage treatment process can be reduced, so that the pollution reduction and carbon reduction coordinated synergism target of sewage treatment can be realized.

Method for recycling cyanobacteria mud and kitchen waste by using hermetia illucens larvae

Resumen de: CN119924265A

The invention belongs to the technical field of organic waste recycling, and particularly relates to a method for recycling cyanobacteria mud and kitchen waste by using hermetia illucens larvae. And mixing the pretreated cyanobacteria mud with the kitchen waste to obtain a mixed feed, and adding a bacillus inoculant for aerobic fermentation to obtain hermetia illucens larvae and larva sand. By regulating and controlling the matching of the matrix and the synergistic effect of the fungicide, the nutritional value and the utilization efficiency of the mixed feed are increased, and the black soldier flies grow faster in the early stage. The bred hermetia illucens larvae can be used as a high-quality protein feed, and the larva sand can be used as an organic fertilizer raw material. According to the method disclosed by the invention, collaborative recycling of the cyanobacteria and the kitchen waste is realized, and a biological solution capable of being applied on a large scale is provided for treatment and utilization of the water-blooming cyanobacteria mud.

Culture optimization method for improving microalgae output

Resumen de: CN119931836A

The invention relates to a culture optimization method for improving microalgae output, equipment of the culture optimization method comprises a transparent culture container, a stirring paddle located in the culture container and an auxiliary light source for forming uniform light supplement on the peripheral surface of the culture container, the speed of the stirring paddle is adjustable, and the illumination intensity of the auxiliary light source is adjustable. The culture optimization method mainly comprises three experimental groups, the first experimental group selects stirring paddle stirring blade types, the second experimental group selects stirring paddle rotating speeds, and the third experimental group selects illumination intensity, so that the culture optimization method is used as an optimization scheme for subsequent microalgae culture, and the yield of microalgae is increased.

Microalgae hydrogel particle in-situ treatment device for nitrogen and phosphorus removal of bay water body

Resumen de: CN222834110U

The utility model relates to the technical field of deep nitrogen and phosphorus removal of a water body, in particular to an in-situ treatment device suitable for a bay water body denutrition process, which comprises a support device and a microalgae hydrogel particle bearing device, the supporting frame is used for supporting and fixing the microalgae hydrogel particle bearing device and is fixedly connected with the floating cylinder, and the floating cylinder is fixedly connected with the vertically arranged heavy object so as to realize the positioning of the height in water. According to the water eutrophication in-situ treatment device provided by the utility model, biomass fixation and in-situ nitrogen and phosphorus removal are carried out by arranging the capsule body filled with the microalgae hydrogel particles, and the microalgae hydrogel particles are used as a buffer, so that microalgae biomass grows in the hydrogel, the biomass can be effectively enriched under a certain stress environment condition, and the water eutrophication in-situ treatment device has the advantages that the water eutrophication in-situ treatment effect is improved; the problems that biomass of a traditional biological membrane is lost and the integrity of a cell membrane is damaged due to the influence of hydraulic shearing force are solved.

Solid culture medium for microalgae separation culture and microalgae separation method

Resumen de: CN119931838A

The invention provides a solid culture medium for microalgae separation culture and a microalgae separation method, and belongs to the technical field of algae separation, the solid culture medium provided by the invention comprises the following components: 30-40 g/L of agar, 0.2 g/L of KNO3, 0.02 g/L of KH2PO4, 0.005 g/L of FeSO4. 7H2O, 0.02 g/L of EDTANa2, 0.0005 g/L of MnSO4, 0.04 g/L of Na2SiO3, 6 mu g/L of vitamin B1, and 50 ng/L of vitamin B12. When the solid culture medium and the separation method provided by the invention are used for separating the microalgae, the separation time of the microalgae can be greatly shortened, the purity of the microalgae is improved, high-quality microalgae resources are provided for subsequent research, large-scale culture and application of the microalgae, and the solid culture medium and the separation method have wide application prospects and important practical values in the related fields of the microalgae.

Multi-channel tubular photo-oxidation bioreactor device

Resumen de: CN222821568U

The utility model discloses a multichannel tubular photo-oxidation bioreactor device, which relates to the technical field of microalgae culture and online monitoring and comprises a water bath box, a gas regulation and control module, a dissolved oxygen monitoring module, an optical density monitoring module and a PH (potential of hydrogen) monitoring module. A glass culture tube and a temperature control module are mounted in the water bath box, the dissolved oxygen monitoring module extends into the glass culture tube through a probe or a sensor, and the optical density monitoring module is in contact with the glass culture tube through an optical sensor or a probe, so that accurate culture conditions are provided, and the microalgae culture efficiency is improved; according to the device, a controllable algae culture environment is provided, meanwhile, real-time monitoring of dissolved oxygen, OD value, PH and temperature is achieved, collection and analysis of scientific research data are facilitated, a user can conveniently set a culture program and check measurement data through a graphical interface of a control system, and the device is convenient to install and use due to the compact machine body structural design.

SYSTEM AND METHOD FOR CULTIVATING AND HARVESTING CYANOBACTERIAL BIOMASS

Resumen de: US2025136922A1

Disclosed is a system for cultivating and harvesting a cyanobacterial biomass. The system comprises at least one vessel that, when in operation, grows the cyanobacterial biomass in a nutrient growth media under regulated growth conditions and a base unit configured to receive the at least one vessel. The system also comprises at least one light source and a cultivation air pump that, when in operation, supplies light, heat and air to the at least one vessel. Notably, the cultivation air pump supplied air via a one-way air valve in the at least one vessel. The system also comprises at least one photodiode that, when in operation, measure a cyanobacterial biomass concentration in the at least one vessel. Disclosed also is a method for cultivating and harvesting a cyanobacterial biomass.

CHLORELLA MICROALGA AND USE THEREOF IN WASTEWATER TREATMENT

Resumen de: WO2025086320A1

Provided are a Chlorella microalga and the use thereof in wastewater treatment, the microalga being Chlorella sp., and the accession number thereof being CCTCCM 20231952. The wastewater includes municipal wastewater, food processing wastewater, printing and dyeing industrial wastewater and animal husbandry breeding wastewater.

BIO-BASED TAURINE PRODUCTION

Resumen de: US2025137018A1

The present invention describes methods for the fermentative production of taurine-containing products from unicellular organisms. More particularly, the invention relates to genetic modifications of the taurine and/or substrate biosynthetic pathways in unicellular organisms that include bacteria, algae, microalgae, diatoms, yeast, or fungi. The invention also relates to fermentation and processing methods for the production of various taurine-containing products. The invention also relates to the use of the cells, fermentation broth or extracts that contain taurine to produce products for use in food, feed, beverages, dietary and health supplements, cosmetics, personal care, pharmaceuticals, or agricultural production.

Active cyanobacteria dry land fertilizer as well as preparation method and application thereof

Resumen de: CN119899054A

The invention belongs to the technical field of agricultural biology and the technical field of compound fertilizers, and discloses an active cyanobacteria dry land fertilizer and a preparation method and application thereof.The method comprises the following steps that cyanobacteria is cultured and harvested to obtain cyanobacteria concentrated pulp, then the cyanobacteria concentrated pulp is rinsed with a drying protective agent, and the active cyanobacteria dry land fertilizer is obtained; preferably, symbiotic rhizobium is added into the cyanobacteria concentrated slurry, and the cyanobacteria concentrated slurry is mixed with a matrix material to obtain a cyanobacteria embedding material; then drying and crushing the cyanobacteria concentrated slurry or the cyanobacteria embedding material to obtain cyanobacteria dry powder; and adding the cyanobacteria dry powder into a plant fertilizer or an edible fungus fertilizer or water or a soil improvement product to obtain the active cyanobacteria dry land fertilizer. Based on adsorption and embedding of plant growth promoting substances contained in cyanobacteria by the drying protective agent, the active cyanobacteria dry land fertilizer has the advantages of being resistant to storage and transportation, capable of remarkably promoting plant growth, suitable for various field environments, capable of improving soil and increasing fertility and the like.

Self-control type food wastewater advanced treatment reactor based on algal membrane and use method of self-control type food wastewater advanced treatment reactor

Resumen de: CN119898922A

The invention discloses an algal membrane-based self-controlled food wastewater advanced treatment reactor and a use method, and relates to the field of food production wastewater treatment, and the algal membrane-based self-controlled food wastewater advanced treatment reactor comprises a water absorption room, a microalgae biological treatment unit, an ultrafiltration membrane separation unit, an effluent and biomass collection device and a computer control system. The secondary treatment effluent of the food processing wastewater flows into the water absorption chamber through the water inlet pipe, most of original suspended matters are removed through the ceramsite layer and the gravel layer, the effluent enters the microalgae biological treatment unit through the water absorption pipe, and main residual pollutants such as nitrogen, phosphorus and organic matters in the secondary treatment effluent of the production wastewater are removed in the microalgae biological treatment unit. Meanwhile, microalgae utilize nutrient substances in the wastewater to accumulate biomass, and after the microalgae biomass is accumulated to a certain level, algae liquid of the microalgae biological treatment unit enters the ultrafiltration membrane separation unit to separate the microalgae from the wastewater, so that the traditional MBR treatment process is optimized, the occupied space is reduced, and the wastewater treatment efficiency is improved. The method has a wide application pros

Polysaccharide-producing talsalonema and application thereof in treatment of high-nitrogen and high-phosphorus wastewater

Resumen de: CN119875842A

The invention discloses a strain of polysaccharide-producing Trichothrix sp. And application of the polysaccharide-producing Trichothrix sp. ZSL-NS5 in high nitrogen and phosphorus wastewater treatment. The strain named as the Trichothrix sp. ZSL-NS5 is preserved in the China Center for Type Culture Collection on November 7, 2024, and the preservation number is CCTCC NO.M 20242470. The invention further discloses a preparation method of the polysaccharide-producing Trichothrix sp. ZSL-NS5. The application of the polysaccharide-producing Trichothrix sp. ZSL-NS5 in the high nitrogen and phosphorus wastewater treatment of the polysaccharide-producing Trichothrix sp. The algal strain is separated from wastewater of an aerobic pond of a dairy farm, has relatively strong wastewater tolerance and pollutant purification capacity, and has a total phosphorus removal rate of more than 95%, an ammonia nitrogen removal rate of nearly 100%, a TN removal rate of more than 85% and a COD removal rate of more than 70%. Besides, the talsalonema has the capability of producing microalgae EPS and polysaccharide, the polysaccharide content of the talsalonema reaches 63%, the yield of the talsalonema is obviously higher than that of common microalgae, and the talsalonema has the application value of preparing chemical raw materials and drugs.

Microalgae culture method

Resumen de: CN119875841A

The invention relates to a microalgae culture method, which comprises the following steps: carrying out first culture on a first microalgae species, and separating the cultured material to obtain a first culture clear liquid; a second microalgae species and the first culture clear liquid are mixed, second culture and third culture are sequentially carried out, and the third culture is carried out in the presence of a grease inducer. According to the method disclosed by the invention, the grease content of a microalgae product can be remarkably improved on the premise of not influencing the microalgae biomass accumulation level, and the utilization value of microalgae biomass is improved.

Method for improving key enzyme to promote euglena to degrade phenol and enhance carbon sequestration

Resumen de: CN119875986A

The invention discloses a technology for degrading phenol and sequestering carbon by microalgae, and aims to provide a method for improving key enzyme to promote euglena to degrade phenol and enhance carbon sequestration. Comprising the following steps: performing phenol concentration gradient domestication on euglena cells, and adjusting gene expression quantities of three key enzymes, namely H < + > transport ATP enzyme, pyruvate decarboxylase and isocitrate dehydrogenase in the euglena cells; carrying out enlarged culture growth on the euglena domesticated plant, adding a proper amount of phenol into a culture medium, and continuously introducing gas containing CO2; in the culture process, the carbon sequestration rate of the euglena is calculated by measuring the carbon element content in the biomass of the euglena liquid; and after the culture is finished, measuring the content of euglena polysaccharide in the algae liquid in unit volume, and measuring the content of phenol in the algae liquid to calculate the degradation rate of phenol. According to the method, the euglena cells are domesticated by adopting phenol concentration gradient, the expression quantity of key enzyme genes is increased by several times, the phenol degradation capacity of the euglena cells is enhanced, the carbon sequestration rate of the euglena cells is increased, and the content of polysaccharide in the cells can be increased at the same time.

Brevibacillus laterosporus GBW-M0 and application thereof in aquaculture

Resumen de: CN119875879A

The invention provides brevibacillus laterosporus GBW-M0 and an application of the brevibacillus laterosporus GBW-M0 in aquaculture. The brevibacillus laterosporus GBW-M0 is classified and named as brevibacillus laterosporus, and the preservation number of the brevibacillus laterosporus GBW-M0 is CGMCC (China General Microbiological Culture Collection Center) No. 32335. The brevibacillus laterosporus GBW-M0 is subjected to seed solution preparation and immersion fermentation, and brevibacillus laterosporus GBW-M0 fermentation liquor is prepared. The brevibacillus laterosporus GBW-M0 fermentation liquor can reduce the concentration of enterocytozoon hepatopenaeus carried in the bodies of penaeus vannamei, is further used for preventing and controlling microsporidiosis of aquaculture animals, and can also inhibit the growth of microcystis aeruginosa, so that the brevibacillus laterosporus GBW-M0 fermentation liquor is used for treating cyanobacterial bloom in aquaculture water, and the success rate of aquaculture is improved.

Photo-biological circulating fixed bed reaction system and method for purifying urine based on microalgae

Resumen de: CN119874042A

The invention belongs to the technical field of domestic sewage treatment, and particularly relates to a photobiological circulating fixed bed reaction system and method for purifying urine based on microalgae, and the photobiological circulating fixed bed reaction system comprises a circulating fixed bed reactor, a lighting system, an aeration system and a liquid driving system, the photo-biological circulating fixed bed reaction system consists of a circulating fixed bed reactor, a lighting system, an aeration system and a liquid driving system; clinoptilolite is used as a filler of the fixed bed area, the concentration of ammonia nitrogen in the urine can be greatly reduced in the cyclic adsorption process, so that a favorable environment is provided for growth of microalgae, the concentration of the ammonia nitrogen in the treated urine is reduced to a quite low level, and under the action of concentration gradient pressure, the concentration of the ammonia nitrogen in the urine is reduced to a quite high level. The clinoptilolite filler loaded with ammonia nitrogen can desorb ammonia nitrogen into urine, a nitrogen source is provided for growth of microalgae, and meanwhile regeneration of the clinoptilolite filler is achieved in the process.

Artificial crust compound biological agent and use method thereof

Resumen de: CN119875937A

The invention discloses an artificial crust compound biological agent and a use method thereof, and aims to solve the problem of wound ecological restoration in Hexi corridor engineering construction. The compound biological agent is composed of a microalgae and artemisia desertorum gum mixed solution and bacillus powder, the microalgae comprise microcoleus vaginatus, pseudocladosporium javanicum and championia gracilis, the microalgae and the artemisia desertorum gum are mixed according to a specific proportion and then are compatible, and bacillus subtilis or bacillus mucilaginosus can be selected as the bacillus powder. When in use, the bacillus powder is firstly scattered, and then the microalgae artemisia desertorum gum mixed solution is sprayed. The compound biological agent provided by the invention can significantly improve the crust thickness, biomass and compressive strength of the sand soil, reduce the wind erosion rate, increase the contents of nitrogen, phosphorus, potassium and organic matters in the soil, effectively improve the ecology of the sand soil, and provide a new technology for wind erosion prevention and ecological restoration of the engineering slash in the arid region.

Natural reoxygenation enhanced nitrogen and phosphorus removal cascade artificial wetland system

Resumen de: CN119874043A

The invention relates to the technical field of constructed wetlands, in particular to a natural reoxygenation enhanced nitrogen and phosphorus removal cascade constructed wetland system. The system is provided with a first-stage constructed wetland, a second-stage ecological pond and a third-stage constructed wetland which are sequentially connected in a falling manner, wetland plants are planted in the first-stage constructed wetland and the third-stage constructed wetland; ecological pond plants are planted in the second-stage ecological pond through floating rafts, non-woven fabric is arranged below the floating rafts, and microalgae are enriched on the non-woven fabric; a mixed matrix of volcanic rock, zeolite and magnesite is arranged in the first-stage constructed wetland, a mixed matrix of volcanic rock and zeolite is arranged in the third-stage constructed wetland, and the effluent part of the second-stage ecological pond flows back to the front end of the first-stage constructed wetland. By means of a series of treatment modes, removal of nitrogen and phosphorus is enhanced, the removal rates of ammonia nitrogen, nitrate nitrogen, total nitrogen, COD and total phosphorus can reach 98.8%, 99.2%, 99.0%, 93.52% and 94.0% respectively, and the method has wide application prospects.

Acinetobacter with algal inhibition capability and application of acinetobacter

Resumen de: CN119875900A

The invention discloses acinetobacter with algal inhibition capability and application thereof, and relates to the technical field of microorganism and environmental organic pollutant repair and governance, the acinetobacter with algal inhibition capability is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO.65654. The acinetobacter with algal inhibition capability is preserved in Guangdong Microbial Culture Collection Center; the 16SrDNA nucleotide sequence of the acinetobacter is shown as SEQ ID NO: 7, and the acinetobacter is applied to treatment of cyanobacterial bloom. The method comprises the following steps: extracting an extracellular culture of cylindrotheca, culturing on a beef extract peptone solid culture medium, extracting a single colony from a culture product, preserving, inoculating into a beef extract peptone liquid culture medium, continuously culturing, and carrying out PCR (Polymerase Chain Reaction) amplification on bacteria separated from the culture product by adopting a specific primer pair. The obtained amplification product is subjected to sequencing and contrastive analysis, bacteria are identified to be the acinetobacter with the algae inhibition capacity, the growth of blue-green algae can be effectively inhibited, and the method has the advantages of being environmentally friendly, low in cost and remarkable in algae control effect.

Probiotic Formulations for Improving Athletic Performance

Resumen de: US2025127827A1

A probiotic formulation is provided including one or more bacteria, bacterial strains or bacterial species of the genus Veillonella, genus Faecalibacterium, genus Phascolarctobacteria, genus Oscillospira, genus Ruminococcus, genus Bacteroides, genus Blautia, family Christensenellaceae, genus Dialister, or phylum cyanobacteria.

HIGH-PROTEIN MICROALGAL BIOMASS HAVING EXCELLENT PEPSIN DIGESTIBILITY, CULTURE METHOD AND USE THEREOF

Nº publicación: US2025129325A1 24/04/2025

Solicitante:

CJ CHEILJEDANG CORP [KR]
CJ CheilJedang Corporation

Resumen de: US2025129325A1

The present disclosure provides high-protein microalgal biomass, a culture method and use thereof.