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Method for cooperatively fattening oysters by using composite microalgae and lactic acid bacteria

Resumen de: CN122096025A

The invention discloses a method for cooperatively fattening oysters by utilizing compound microalgae and lactic acid bacteria. The method comprises the following steps: temporarily culturing the oysters; the method comprises the following steps: selecting chlorella vulgaris, Platymonas Qingdao, skeletonema costatum and Chaetoceros mulleri as composite microalgae, and carrying out expanding culture on each microalgae in an f/2 culture medium to a logarithmic phase; lactobacillus plantarum is used as a probiotic agent, and activation and amplification culture are carried out; then collecting and concentrating the thalli; preparing a bacterial suspension for later use; mixing the composite microalgae liquid and the lactobacillus plantarum suspension in a fermentation container, and fermenting in a standing manner; and then feeding the fermented mixed liquid twice a day, taking out the oysters, and finishing fattening. According to the method, through the synergistic effect of the microalgae and the lactic acid bacteria, the fatness, the protein content, the total sugar content, the triglyceride content and the unsaturated fatty acid content of the oysters can be remarkably improved, meanwhile, the fattening cost is effectively reduced, the weight increment of the oysters can be accelerated, the meat yield can be increased, the aquatic water can be purified through the metabolic activity of the microalgae, and nitrogen and phosphorus accumulation in the water can be reduced.

Application of pyrroloquinoline quinone in relieving cyanobacteria toxin poisoning of pelteobagrus fulvidraco

Resumen de: CN122096291A

The invention discloses application of pyrroloquinoline quinone (PQQ) or derivatives thereof in preparation of products for relieving or preventing and treating cyanobacteria toxin poisoning of pelteobagrus fulvidraco, and belongs to the technical field of aquatic animal feed. The PQQ or the derivative thereof can be used as a feed additive or medicine for aquatic products, is added and fed according to 3-6mg/kg of feed, and effectively relieves the pathological liver injury, caused by cyanobacteria toxin, of the pelteobagrus fulvidraco through various ways of improving the activity of liver function key enzymes, inhibiting liver oxidative stress, relieving inflammatory response, relieving structural tissue injury and the like. As a new food raw material approved in China in 2022, the PQQ has the advantages of high safety, good water solubility, moderate cost, similar feed vitamin dosage and the like, provides a safe, economic and convenient-to-apply novel nutrition intervention strategy for blue-green algae toxin poisoning of the pelteobagrus fulvidraco, and has a wide industrial application prospect.

Microalgae circulating precipitation concentration harvesting device and harvesting method thereof

Resumen de: CN122104390A

The invention belongs to the technical field of microalgae concentration, and discloses a microalgae circulating precipitation concentration harvesting device and a harvesting method.The microalgae circulating precipitation concentration harvesting device comprises a shading circulating precipitation concentration barrel, a liquid outlet pipe is communicated with one side wall of the top of the circulating precipitation concentration barrel, and a liquid inlet pipe is communicated with one side wall of the circulating precipitation concentration barrel; the liquid inlet pipe is positioned below the liquid outlet pipe on the circulating precipitation concentration barrel; the exterior of the liquid inlet pipe is communicated with a power pump, and the water inlet end of the power pump is communicated with an external algae liquid culture device through a pipeline. The liquid inlet flow is controlled by adjusting the power pump, so that the rising speed of the liquid level in the circulating precipitation concentration barrel is smaller than the dark precipitation speed of algae cells, the algae cells can be gradually precipitated and concentrated at the conical bottom of the circulating precipitation concentration barrel in the circulation process of the algae liquid, and the concentrated algae liquid can be collected through the sampling pipe at the bottom of the circulating precipitation concentration barrel. According to the invention, the purposes of continuous circulating

Method for preparing calcium carbonate microcrystal through mineralization of microalgae regulated by carbonic anhydrase, calcium carbonate microcrystal and application of calcium carbonate microcrystal

Resumen de: CN122104815A

The invention discloses a method for preparing calcium carbonate microcrystals through carbonic anhydrase-regulated microalgae mineralization, the calcium carbonate microcrystals and application of the calcium carbonate microcrystals. The method comprises the following steps: S1, microalgae culture and grease accumulation: carrying out multiplication culture on gloeococcus mucilaginosus to enable algae cells to reach a logarithmic end phase or a stable phase; then carrying out nitrogen limitation or nitrogen starvation induction culture to accumulate grease of the algae; s2, mineralization induction culture: transferring the algae liquid into a mineralization culture medium, adding carbonic anhydrase, and culturing for 5-48 hours; the mineralization culture medium contains Ca < 2 + > and HCO3 <->; s3, microcrystal collection: harvesting the algae subjected to mineralization induction culture, and separating microcrystals to obtain calcium carbonate microcrystals. The calcium carbonate microcrystal obtained by the method can be quickly converted into carbonic acid doped hydroxyapatite, and is further applied to 3D printing medical materials. The method is combined with a microalgae low-nitrogen induction process, and co-production of a biomineralization material and high-fat algae powder can be realized.

EXTRACT FROM ALGA AGAINST PLANT PATHOGENIC BACTERIA

Resumen de: HUP2400501A1

The subject of the invention is a unicellular microalgal culture for use against fire blight or tomato, tobacco, pepper and potato rot. The invention also relates to the use of an organic solvent extract of the algal culture containing PUFA fatty acids.

NOVEL USE OF AN EXTRACT OF THE MICROALGA PHAEODACTYLUM TRICORNUTUM

Resumen de: WO2025017264A1

The invention relates to a Phaeodactylum tricornutum extract or to a composition comprising same for facilitating the resumption of a physical activity, advantageously a sports activity, and/or reducing cardiovascular risks and/or risks related to fragile bones and/or joints when resuming a physical activity, advantageously a sports activity.

Efficient extraction and preparation method of chlorella protein based on deep eutectic solvent DES

Resumen de: CN122080111A

The invention belongs to the technical field of microalgae protein extraction, and relates to an efficient chlorella protein extraction and preparation method based on a deep eutectic solvent DES, which comprises the following steps: (1) mixing chlorella powder, a DES hydrogen bond acceptor and water, and carrying out ultrasonic-assisted extraction, centrifugation and filtration to obtain an extracting solution; (2) adding a precipitant into the extracting solution for precipitation; and (3) purifying and freeze-drying the precipitate to obtain the chlorella protein. According to the method, the cell walls of the chlorella are efficiently destroyed based on a DES system in combination with ultrasonic energy, protein dissolution is promoted, the extraction rate of the chlorella protein is increased, and the extraction rate of the chlorella protein is further increased by selecting a DES hydrogen bond receptor and optimizing the ratio of DES to chlorella powder, the material-liquid ratio, the ultrasonic intensity and the time; the method is mild in process, simple to operate, recyclable in solvent, environment-friendly and suitable for large-scale production; the extracted protein keeps natural conformation and activity, and is suitable for development of high value-added products.

Device and method for recovering nitrous oxide from high-ammonia-nitrogen wastewater by utilizing phycomycete symbiosis technology

Resumen de: CN122079378A

The invention provides a device and a method for recovering nitrous oxide from high-ammonia-nitrogen wastewater by utilizing a phycomycete symbiosis technology. The device provided by the invention comprises an aerobic reaction unit and an anoxic reaction unit, a first water outlet of the aerobic reaction unit is communicated with a water inlet of the anoxic reaction unit, and a univalent anion exchange membrane is arranged at the communication part; the aerobic reaction unit is communicated with the first gas collection unit through a first gas output port, and the anoxic reaction unit is communicated with the second gas collection unit through a second gas output port and is used for recycling nitrous oxide generated by the aerobic reaction unit and the anoxic reaction unit. The aerobic reaction unit is inoculated with the algal-bacterial symbiotic sludge for realizing short-cut nitrification, the aeration energy consumption of short-cut nitrification is effectively reduced through microalgae photosynthesis, nitrous oxide in the aerobic reaction unit is recovered, the denitrification load of the anoxic reaction unit is reduced, the denitrification conversion rate is increased, and the operation cost is reduced; and the design concept of high efficiency, low carbon and environmental protection is embodied.

Biological stimulant prepared by utilizing aerial microalgae Pilidiococcus sp. FDA-4 and application of biological stimulant

Resumen de: CN122081076A

The invention discloses a biological stimulant prepared by utilizing aerial microalgae Pilidiococcus sp. FDA-4 and application of the biological stimulant, and belongs to the technical field of agricultural biotechnology and environmental microorganism application. The biological stimulant is prepared by culturing aerial microalgae under a specific stress condition, crushing and extracting active ingredients. Experiments show that the salt stress resistance of crops can be remarkably improved by applying the salt stress resistant agent to crop seeds in a vernalization stage: in a 0.5% NaCl salt stress environment, the germination potential of arabidopsis seeds is improved to 65.74%, and the length of main roots of seedlings is doubled compared with that of a salt stress group. The preparation technology is green and efficient, inhibition of soil salinization on crop growth can be effectively relieved, a novel solution is provided for saline-alkali soil improvement and crop yield increase, and good application prospects are achieved.

Liquid microalgae fertilizer capable of reducing cadmium content of rice

Resumen de: CN122079703A

The invention discloses a liquid microalgae fertilizer capable of reducing the cadmium content of rice. The liquid microalgae fertilizer capable of reducing the cadmium content of the rice is prepared from the following components in percentage by mass: 50 to 75 percent of concentrated algae liquid, 5 to 12 percent of humic acid, 2 to 8 percent of potassium fulvate, 0.1 to 0.5 percent of xanthan gum, 0.1 to 0.5 percent of sodium alginate and the balance of water.

Preparation method of nano cellulose selenium-protecting tablet

Resumen de: CN122074668A

The invention relates to a preparation method of nanocellulose selenium-protecting tablets, and belongs to the technical field of health-care foods and pharmaceutical preparations. Aiming at the problems of poor selenium element stability, non-uniform bioavailability, poor taste and the like of the existing selenium-rich supplement, the invention provides a solution which takes natural nano cellulose as an embedding material and a skeleton. According to the technical scheme, the method comprises the following steps: preparing a nanocellulose dispersion liquid; mixing with selenium-enriched pellet microalgae to form an embedding mixed solution; performing vacuum freeze drying and low-temperature crushing to obtain composite powder; mixing the composite powder with konjac powder, a filling agent, a disintegrating agent, a flavoring agent and a lubricating agent; and finally pressing into tablets. The method is mainly used for preparing a selenium-rich tablet product which is high in stability, high in bioavailability, good in taste and easy to take.

Wastewater purification device for immobilized beneficial microorganisms and algal-bacterial symbionts

Resumen de: CN224279951U

The utility model relates to the technical field of wastewater purification, in particular to an immobilized beneficial microorganism and algal-bacterial symbiont wastewater purification device which comprises a floating box, a floating mechanism is arranged on the floating box, a mounting groove is formed in the top end of the floating box, a net-shaped frame is arranged on the mounting groove, a microorganism immobilization carrier is filled in the net-shaped frame, and the microorganism immobilization carrier is arranged in the mounting groove. A woven nylon net is arranged at the top end of the net-shaped frame, microalgae are loaded in the woven nylon net, and a through groove is formed in the bottom end of the floating box. According to the structure, through the synergistic effect of bacteria and algae, organic matter can be effectively removed, eutrophication factors such as nitrogen and phosphorus can be synchronously removed, and certain adsorption and enrichment effects on heavy metal are achieved; the comprehensive wastewater treatment effect is improved, the pressure of a subsequent deep treatment link is reduced, and the overall treatment cost is reduced.

Preparation method of schizochytrium limacinum DHA based on pH response type microcapsules and spray freeze drying preparation system of schizochytrium limacinum DHA

Resumen de: CN122074660A

The invention relates to the field of microalgae functional component preparation, in particular to a schizochytrium limacinum DHA (docosahexaenoic acid) preparation method based on a pH response type microcapsule and a spray freeze drying preparation system of the schizochytrium limacinum DHA. By combining a synergistic process of nano atomization, liquid nitrogen shock cooling, vacuum sublimation drying and the like, the problems that the schizochytrium limacinum DHA microcapsule is easy to oxidize and inactivate, gastrointestinal tract release is not targeted, and a traditional freeze-drying process is long in period and low in efficiency are solved.

Multi-pond constructed wetland system construction method based on rhizosphere synergistic symbiosis and application thereof

Resumen de: CN122079359A

The invention provides a multi-pond constructed wetland system construction method based on rhizosphere synergistic symbiosis and application thereof, and relates to the technical field of ecological environment remediation, and the method specifically comprises the following steps: respectively preparing an engineering algal strain A with laccase displayed on the surface and an engineering algal strain B with non-specific nuclease displayed on the surface; mixing the two according to a ratio to prepare a composite microalgae microbial inoculum; inoculating the prepared composite microalgae fungicide into a shallow pond at the front end of a wetland to construct a photocatalytic microalgae pretreatment unit; submerged plants are planted in symbiotic ponds which are arranged in series after pretreatment units, and bacterial laccase and non-specific nuclease are anchored on the cell surfaces of different synechococcus by adopting a multi-pond artificial wetland system construction method for rhizosphere synergetic symbiotic and utilizing a synthetic biology surface display technology, so that the synechococcus can be rapidly and efficiently constructed. The problems that the matrix adsorbs phosphorus and is easy to saturate and short in service life due to insufficient carbon source when the existing constructed wetland is used for treating tail water of a sewage plant are solved, and the defects of the existing single ecological restoration technology are overcome.

Microalgae capable of adsorbing bornyl acetate chitosan nanoparticles as well as preparation method and application of microalgae

Resumen de: CN122070937A

The invention relates to the technical field of biological medicine, in particular to microalgae adsorbing bornyl acetate chitosan nanoparticles and a preparation method and application thereof. The microalgae capable of adsorbing the bornyl acetate chitosan nanoparticles disclosed by the invention comprises the following components: bornyl acetate, chitosan and spirulina platensis, the bornyl acetate is wrapped in the chitosan, and the bornyl acetate and the chitosan jointly form composite nanoparticles; the composite nanoparticles are adsorbed on the surface of the spirulina platensis through electrostatic interaction. Compared with single bornyl acetate, the microalgae adsorbing the bornyl acetate chitosan nanoparticles has a better anti-inflammatory effect and an intestinal retention effect.

KGP1-12 Novel Chlorella sp. KGP1-12 microalgae that produces high levels of polyhydroxyalkanoate and method for inducible production of polyhydroxyalkanoate using thereof

Resumen de: KR20260071903A

본 발명은 폴리-3-하이드록시부티레이트(Poly-3-hydroxybutyrate, PHB) 및 이를 단량체로 하는 폴리하이드록시알칸산(Polyhydroxyalkanoate, PHA) 생산에 이용되는 신규 클로렐라 속 KGP1-12 미세조류에 관한 것으로, 보다 상세하게는 신규 클로렐라(Chlorella sp.) KGP1-12 미세조류; 상기 미세조류를 포함하는 폴리-3-하이드록시부티레이트 및/또는 폴리하이드록시알칸산 생산용 조성물; 상기 미세조류를 배양하여 폴리-3-하이드록시부티레이트 및/또는 폴리하이드록시알칸산을 생산하는 방법을 제공한다.

Biomass-recoverable bacteria-algae membrane aeration biological membrane sewage treatment equipment and application thereof

Resumen de: CN122059539A

The invention provides a biomass recoverable bacteria-algae membrane aeration biological membrane sewage treatment device and application thereof. The system comprises an internal and external bidirectional lighting device, a ferromagnetic microalgae collection scraper, a pool wall cleaning device, and an annular membrane aeration biological membrane, a micro-aeration device and a water circulation device which are arranged in a cylindrical reactor. The two-way lighting devices are arranged on the inner side and the outer side of the ring layer, microalgae grow on the ring layer annular biological membrane assembly in an attached mode, a strong magnetic microalgae collecting scraper blade is specially arranged, and the micro-aeration device and the water circulation device are arranged below the device and are in a ring-shaped uniform water distribution mode. The system is used for treating high-ammonia-nitrogen organic wastewater and also can be used for treating wastewater containing novel pollutants such as antibiotics, compared with the prior art, the system has the advantages of uniform water distribution, good light transmission, high space utilization rate, convenience in installation and the like, and the whole system is stable and efficient in operation and good in sewage treatment effect and has a very good development and application prospect.

Method integrating microalgae lipid accumulation promotion, algae cell flocculation and lipid extraction

Resumen de: CN122059871A

The invention discloses a method integrating microalgae lipid accumulation promotion, algae cell flocculation and lipid extraction, and belongs to the field of bioengineering and energy. An AIE group is used as a main body structure, positive charges and hydrophobic chains are modified through quaternization, so that AIE molecules can be combined with algae cells through electrostatic interaction and hydrophobic interaction, and lipid accumulation of the algae cells is promoted by improving the light energy utilization rate of the algae cells. Furthermore, integration from lipid synthesis to extraction is realized based on the positive charge and active oxygen generation performance of molecules.

Device for continuously culturing fresh water microalgae indoors at constant temperature

Resumen de: CN224258605U

The utility model provides a device for continuously culturing fresh water microalgae indoors at constant temperature. According to the device, a cross beam and a hanging frame are installed at the top of each main frame, breeding bags are correspondingly hung on the two sides of each hanging frame, a pH value detector and an air inlet pipe are arranged in each breeding bag, a monitoring alarm box is installed on the main frame at the end, and an upper light bar and a lower light bar are installed on the two sides of each main frame and correspond to each breeding bag. Sewage discharge grooves are formed in the ground at the two outer ends of the main frame, the bottom of each culture bag is communicated with a liquid conveying mechanism, and an algae liquid conveying mechanism is arranged on the ground at the inner end of the main frame. The buffer tank is provided with a non-contact liquid level monitor, a material pipe, a material pipe electromagnetic valve and a buffer tank electromagnetic valve; and the main conveying pipe is provided with a segmented electromagnetic valve, a connecting straight pipe and a branch pipe electromagnetic valve. According to the device, the culture bags are connected in series to culture the freshwater microalgae, the liquid conveying mechanism is used for unified conveying, the structure is simple and practical, the automation degree is high, the equipment investment cost is low, and the yield of the freshwater microalgae is high.

Fungus fungicide and application thereof in algae crust

Resumen de: CN122060501A

The invention discloses a fungal inoculant and application thereof in algae crust, and belongs to the field of microorganisms. The technical problem to be solved by the invention is how to construct the algae crust. The application of the fungal inoculant in the algal crust comprises the following steps: inoculating deep-color endophytic fungi and microalgae to the surface layer of soil to be remediated, and culturing to realize the construction of the algal crust; the deep-color endophytic fungi are bacterial strains with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.17463 in the China General Microbiological Culture Collection Center; the microalgae is composed of Microcoleus sp., Scenedesmus sp. And Chlorella sp., and the microcoleus sp., the Scenedesmus sp. And the Chlorella sp. Are used in the method. According to the method, rapid formation of algae crust and improvement of soil carbon accumulation are successfully realized, the key technical problems of long crust period, poor adaptability and the like are solved, and the purpose of ecological restoration of degraded soil in an arid and semi-arid mining area is favorably realized.

Method for preparing the microalgae-derived exosome analogue and composition for improving immunity comprising the same

Nº publicación: KR20260070008A 18/05/2026

Solicitante:

RESEARCH & BUSINESS FOUNDATION SUNGKYUNKWAN UNIV [KR]
\uC131\uADE0\uAD00\uB300\uD559\uAD50\uC0B0\uD559\uD611\uB825\uB2E8

Resumen de: KR20260070008A

본 발명은 미세조류 유래 엑소좀 유사체 제조방법, 이로부터 제조된 엑소좀 유사체 및 이를 포함하는 면역 증강용 조성물에 관한 것으로, 본 발명의 방법은 미세조류 유래 엑소좀 유사체를 효율적으로 생산할 수 있으며, 본 발명의 미세조류 유래 엑소좀 유사체는 베타글루칸을 포함하고 있는 바, 베타글루칸의 낮은 용해도 문제를 해결할 수 있으며 면역 증강 효과가 우수하여 의약품, 건강기능식품 또는 화장료 조성물로 활용될 수 있다.