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Nano antibacterial peptide based on leucine zipper as well as preparation method and application of nano antibacterial peptide

Resumen de: CN121471323A

The invention provides a nano antibacterial peptide based on a leucine zipper and a preparation method and application thereof, and belongs to the technical field of biology, and the amino acid sequence of the nano antibacterial peptide is as shown in SEQ ID No.1. The antibacterial peptide disclosed by the invention has self-assembly capability, can be assembled to form a compact nanofiber net, has an antibacterial effect on gram-negative bacteria including escherichia coli, pseudomonas aeruginosa and salmonella typhimurium and gram-positive bacteria including staphylococcus aureus, staphylococcus epidermidis and enterococcus faecalis, and meanwhile, has relatively high biocompatibility, so that the antibacterial peptide can be used for preparing the antibacterial peptide. Moreover, the antibacterial peptide disclosed by the invention can stably exert an antibacterial effect under the conditions of serum, acid and alkali. In conclusion, the antibacterial peptide disclosed by the invention is an antibacterial peptide with relatively good antibacterial activity and biocompatibility.

High-temperature-resistant and inflammation-relieving Witzia ciliaris CN985 and application thereof

Resumen de: CN121472086A

The invention provides a strain of high-temperature-resistant and inflammation-relieving Witzia ciliaris and an application thereof, the high-temperature-resistant and inflammation-relieving Witzia ciliaris is Witzia ciliaris CN985 and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is April 7, 2024, and the preservation number is CCTCC NO: M 2024641. The invention further provides a preparation method of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain for preventing and treating inflammation of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain for preventing and treating inflammation of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain. The invention provides a brand new strain of Witzia ciliaris CN985, which is resistant to high temperature, has better acid resistance and bile resistance, and has no hemolysis risk when being applied to a human body. The Witzia coagulans CN985 has excellent antibacterial performance, and especially can significantly inhibit streptococcus pyogenes, salmonella enteritidis and/or group B streptococcus; besides, experimental results show that the Witzia ciliaris CN985 can relieve enteritis, improve the immunity of the organism and relieve pharyngitis, and especially when the Witzia ciliaris CN985 and the phytobacterium plantarum CE18 are combined for use, the Witzia ciliaris CN985 and the phytobacterium plant

Bacillus subtilis for recombinant expression of hermetia illucens HIAMP0438 gene and construction method thereof

Resumen de: CN121472283A

The invention discloses bacillus subtilis for recombinant expression of a hermetia illucens HIAMP0438 gene and a construction method of the bacillus subtilis, and belongs to the technical field of gene engineering. The hermetia illucens recombinant protein used in the invention is obtained by expression of bacillus subtilis WB800N transferred into a pHT43-HIAMP0438 high-efficiency expression vector, and the pHT43-HIAMP0438 high-efficiency expression vector is obtained by a homologous recombination technology, so that high-level expression of the HIAMP0438 gene is realized, the HIAMP0438 gene can be efficiently secreted out of cells, the preparation process of the recombinant protein is greatly simplified, and the production cost is reduced. According to the present invention, the recombinant protein can significantly inhibit the growth of Escherichia coli, Salmonella and Listeria monocytogenes, and the recombinant protein can be used as the animal health product, and can be used for preventing and treating pig and chicken intestinal diarrhea caused by Escherichia coli and Salmonella and food-borne digestive tract diseases caused by Listeria monocytogenes.

Antibacterial peptide ATMP23 mutant with improved antibacterial activity and application of antibacterial peptide ATMP23 mutant in preparation of antibacterial agent

Resumen de: CN121471322A

The invention provides an antibacterial peptide ATMP23 mutant with improved antibacterial activity and application of the antibacterial peptide ATMP23 mutant in preparation of an antibacterial agent. The amino acid sequence of the antibacterial peptide ATMP23 provided by the invention is shown as SEQ ID NO: 1, amino acid mutation and screening are carried out on the basis of the amino acid sequence, and antibacterial peptide mutants A23-M4, A23-M5 and A23-M6 with significantly improved antibacterial activity are obtained. The antibacterial peptide ATMP23 mutant provided by the invention is small in molecular weight, convenient to artificially synthesize and low in cost, and has a good bactericidal effect on clostridium welchii, staphylococcus aureus, streptococcus, vibrio parahaemolyticus, vibrio alginolyticus, vibrio splendidus, salmonella, escherichia coli and the like; when being applied to livestock and aquatic feed, the feed conversion ratio of livestock and aquatic products can be remarkably reduced, the growth performance and immune function indexes of bred animals can be improved, and the feed additive can be widely applied to the industries of feed, food, medicine and the like.

一种叶酸-邻苯二胺共掺杂碳量子点、大肠埃希氏菌O157:H7检测探针、检测试剂及应用

Resumen de: CN121471909A

本发明属于食品安全检测技术领域，具体涉及一种叶酸‑邻苯二胺共掺杂碳量子点、大肠埃希氏菌O157:H7检测探针、检测试剂及应用。叶酸‑邻苯二胺共掺杂碳量子点由叶酸和邻苯二胺加热反应制得。基于本发明的叶酸‑邻苯二胺共掺杂碳量子点的检测探针或检测试剂对大肠埃希氏菌O157:H7进行检测，检测限低、特异性优异，实用性强。

一种阪崎克罗诺杆菌核酸适配体及其在制备生物传感器中的应用

Resumen de: CN121472232A

本发明涉及生物技术领域，具体公开了一种阪崎克罗诺杆菌核酸适配体及其在制备生物传感器中的应用。本发明针对阪崎克罗诺杆菌MipA蛋白设计了DNA序列如SEQ ID No:1所示的核酸适配体，5’端氨基修饰。该适配体可用于构建荧光淬灭型生物传感器，包括荧光探针（枸橼酸二铵、硫脲、正硅酸甲酯及二氧化铈合成荧光纳米粒子，第一适配体羧基修饰）和淬灭探针（柠檬酸还原金纳米粒子，第二适配体巯基修饰，SEQ ID No:2）。与传统方法相比，筛选效率显著提升，检测时间<1h，抗干扰强，亲和常数13.28nM，适用于婴幼儿食品快速监测，经济成本降低至少40%。

一种用于检测大肠杆菌的双模态生物传感器检测试剂盒及制备方法

Resumen de: CN121476591A

本发明具体涉及一种用于检测大肠杆菌的双模态生物传感器检测试剂盒及制备方法，属于食品安全检测技术领域。双模态生物传感器检测试剂盒由网状靶向磁珠和生物传感器检测试剂组成；生物传感器检测试剂为适配体修饰的铜金属掺杂碳点溶液，适配体为氨基化适配体。本发明的双模态传感器提供的双信号可以互相校准，避免单信号输出的偶然性误差，还可根据不同的场景选择优势检测模态；以网状PGA结构为中间体包覆四氧化三铁磁珠，高负载的网状结构为识别元件提供更多结合位点进一步增强了磁分离技术的捕获效率；增强磁分离技术驱动的双金属靶向改性碳点在检测大肠杆菌O157:H7时具有良好的检测性能，在食品基质中捕获低浓度目标菌的效率可达90%以上。

A YEAST BIOMASS COMPOSITION AND USES THEREOF

Resumen de: WO2026027798A1

A yeast biomass composition is formed by culturing a strain of yeast in a substrate such as whey permeate to product a yeast broth, dewatering the broth to produce yeast biomass, and hydrolysing the yeast biomass. The yeast biomass composition finds utility as a feed additive for broiler chickens to reduce the adherence of pathogenic bacteria like Salmonella and E.coli to the gut wall of the chickens, thereby reducing intestinal stress and improving life daily weight gain in the chickens.

LIVER DISEASE TREATMENTS USING ATTENUATED STRAINS OF SALMONELLA TYPHIMURIUM

Resumen de: WO2026030756A2

The composition and methods of treatment disclosed or contemplated herein include compositions and methods of treatment for liver-related diseases. The overall treatment strategy would involve the identification of a target protein or receptor within the liver that is responsible for ongoing liver injury. The strain of orally administered Salmonella Typhimurium that colonizes the liver would be engineered to secrete a particular protein or nanobody that would stop and/or reverse the progressive hepatic disease

ANTI-ETEC ADHESIN PROTEIN ANTIBODIES AND METHODS OF USE

Nº publicación: US20260035440A1 05/02/2026

Solicitante:

UNIV OF MASSACHUSETTS [US]
University of Massachusetts

Resumen de: US20260035440A1

The invention provides anti-ETEC adhesin protein antibodies and methods of using the same.