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Lactobacillus rhamnosus FJS004 for inhibiting food-borne pathogenic bacteria as well as lactein and application of lactobacillus rhamnosus FJS004

Resumen de: CN119979385A

The invention relates to the technical field of probiotics, in particular to lactobacillus rhamnosus FJS004 for inhibiting food-borne pathogenic bacteria, lactein of lactobacillus rhamnosus FJS004 and application of lactobacillus rhamnosus FJS004. The lactobacillus rhamnosus FJS004 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 26, 2022, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.26253. The lactobacillus rhamnosus FJS004 can be used for preparing the lactobacillus rhamnosus FJS004. The lactobacillus rhamnosus FJS004 has the advantages that the lactobacillus rhamnosus FJS004 can be used for preparing the lactobacillus rhamnosus FJS004; the lactobacillus rhamnosus FJS004 and the lactein of the lactobacillus rhamnosus FJS004 provided by the invention have a good bacteriostatic or bactericidal effect on escherichia coli, salmonella, shigella, staphylococcus aureus, listeria monocytogenes, pseudomonas aeruginosa, bacillus cereus and enterobacter sakazakii.

Positively charged silicon shell modification-based magnetic fluorescent probe, preparation method and application

Resumen de: CN119979149A

The invention discloses a magnetic fluorescent probe based on positive silicon shell modification as well as a preparation method and application of the magnetic fluorescent probe. The probe adopts superparamagnetic Fe3O4 nanoparticles as an inner core and provides magnetic enrichment capacity; a cationic polymer electrostatically adsorbs multiple layers of quantum dot particles to serve as a fluorescent interlayer, and a fluorescent signal capable of being used for quantitative detection is provided; and a layer of aminated silicon dioxide shell with strong positive electricity is also arranged on the surface, so that the broad-spectrum capturing capability on bacteria with negative electricity is provided. By taking pseudomonas aeruginosa, streptococcus pneumoniae and salmonella typhimurium as examples, rapid enrichment and broad-spectrum quantitative analysis of the pseudomonas aeruginosa, streptococcus pneumoniae and salmonella typhimurium in a sample can be realized, a magnetic enrichment effect and multiple signal amplification of multiple layers of fluorescent quantum dots are realized, matrix interference in practical application is eliminated, and the detection sensitivity is high. The method has great potential in the aspects of high sensitivity and clinical monitoring of bacterial infection or pollution.

Primer probe composition and kit for simultaneously detecting 17 pathogenic microorganisms in food

Resumen de: CN119979776A

The invention discloses a primer probe composition and a kit for simultaneously detecting 17 pathogenic microorganisms in food. The primer probe composition is used for detecting salmonella, staphylococcus aureus, clostridium perfringens, escherichia coli O157, listeria monocytogenes, vibrio parahaemolyticus, bacillus cereus, vibrio cholerae, campylobacter jejuni, clostridium botulinum, proteusbacillus vulgaris, group A rotavirus, norovirus type GI, norovirus type GII and the like. The invention relates to a primer and probe combination for astrovirus, fiveleaf virus and enteric adenovirus. The primer and probe composition disclosed by the invention can be used for efficiently detecting 17 pathogenic microorganisms in food, has the advantages of simple and accurate judgment result, high sensitivity, strong specificity and high precision, and can meet the requirement of food detection industry on rapid detection.

탄수화물-기반 항-살모넬라 백신의 개발

Resumen de: AU2023312737A1

Provided herein are vaccine composition comprising a

Antibacterial peptide Loong-3 and application thereof

Resumen de: CN119978065A

The invention belongs to the technical field of biology, and particularly relates to an antibacterial peptide Loong-3 and application thereof. The sequence of the antibacterial peptide Loong-3 is as follows: lysine, arginine, isoleucine, alanine, lysine, leucine, alanine, leucine, lysine, alanine, isoleucine, arginine, lysine, isoleucine, leucine, arginine, leucine and isoleucine. The antibacterial peptide has better antibacterial activity on gram-positive bacteria (staphylococcus aureus), gram-negative bacteria (escherichia coli and salmonella) and fungi (candida albicans), and has the characteristics of heat resistance, salt resistance, acid and alkali resistance, repeated freezing and thawing resistance, serum resistance and organic solvent resistance. Therefore, the antibacterial peptide Loong-3 has wide application prospects in the fields of preparation of drugs for resisting bacterial and/or fungal infection, animal husbandry development, prevention and treatment of human diseases, food preservation, detergents, cosmetics, antibacterial molecular materials and the like.

CAPTURE COMPOSITIONS

Resumen de: US2025147011A1

Disclosed is a covalently-linked multilayered three-dimensional matrix comprising capture molecules, linkers and spacers (referred to as a Molecular Net) for specific and sensitive analyte capture from a sample. Also disclosed herein is a Molecular Net comprising covalently-linked multilayered three-dimensional matrix comprising more than one type of capture molecule and more than one type of linker and may comprise one or more spacer for specific and sensitive capture of more than one type of analyte from a sample. A Molecular Net may comprise a pseudorandom nature. Use of various capture molecules, linkers and spacers in a Molecular Net may confer unique binding properties to a Molecular Net. Porosity, binding affinity, size exclusion abilities, filtration abilities, concentration abilities and signal amplification abilities of a Molecular Net may be varied and depend on the nature of components used in its fabrication. Uses of a Molecular Net may include analyte capture, analyte enrichment, analyte purification, analyte detection, analyte measurement and analyte delivery. Molecular Nets may be used in liquid phase or on solid phases such as nanomaterials, modified metal surfaces, nanospheres, microspheres, microtiter plates, slides, pipettes, cassettes, cartridges, discs, probes, lateral flow devices, microfluidics devices, microfluidics devices, optical fibers and others.

STREPTOCOCCUS SALIVARIUS SUBSP.THERMOPHILUS VB331 AND USE THEREOF

Resumen de: WO2025092264A1

Provided in the present invention are Streptococcus salivarius subsp.thermophilus VB331 and the use thereof. The strain is deposited at the China General Microbiological Culture Collection Center, with the deposit number of CGMCC NO.28407. The Streptococcus salivarius subsp.thermophilus can effectively inhibit the colonization, propagation and growth of various digestive tract pathogenic bacteria, including Escherichia coli, Staphylococcus aureus, Salmonella, Enterococcus faecalis, Listeria monocytogenes and Shigella, and especially has a strong inhibitory effect on Clostridium perfringens. The strain is a natural strain, is very safe, and can provide a new source of probiotics for the development of antibacterial, bacteriostatic and other functional products against multiple harmful bacterial infections.

Application of T1FR protein in inhibition of Klebsiella pneumoniae and salmonella enteritidis type I pilus

Resumen de: CN119925568A

The invention discloses an application of T1FR protein in inhibition of Klebsiella pneumoniae and salmonella enteritidis I-type fimbriae, and relates to the technical field of biology. Specifically, the T1fr gene is subjected to exogenous expression in klebsiella pneumoniae and salmonella enteritidis, and a biological factor T1FR protein coded by the T1fr gene can inhibit klebsiella pneumoniae and salmonella enteritidis adhesion factor I-type fimbriae, so that adsorption and colonization of pathogenic bacteria in human and animal tissues are inhibited through biological factors, and the use of antibiotics is effectively reduced; a theoretical basis is provided for relieving the drug resistance problem of klebsiella pneumoniae and salmonella, and an important reference scheme is provided for controlling bacterial infection and biofilm formation.

Application of deacetylated NDP52 protein in preparing medicine for controlling salmonella infection

Resumen de: CN119930782A

The invention relates to the technical field of genetic engineering, in particular to application of deacetylated NDP52 protein in preparation of a medicine for controlling salmonella infection. The invention provides a deacetylated NDP52 protein, provides a gene for coding the deacetylated NDP52 protein and a nucleotide sequence of the gene, provides a recombinant vector, a transgenic cell line or a recombinant virus containing the gene, and a substance capable of realizing deacetylation of the NDP52 protein, and further provides the gene, the protein and the recombinant vector containing the gene, the transgenic cell line or the recombinant virus containing the gene and the substance capable of realizing deacetylation of the NDP52 protein. The invention relates to application of a substance for realizing NDP52 protein deacetylation in preparation of salmonella infection control drugs, and provides a scientific basis and a potential tool for treatment of bacterial infection diseases by expressing NDP52 and a deacetylated variant thereof in mouse liver to effectively promote removal of liver bacterial infection and relieve hepatic necrosis.

Application of tannin in preparation of drugs and/or functional products for eliminating displacement and/or recessive infection of salmonella in livers and lungs of poultry

Resumen de: CN119925395A

The invention belongs to the technical field of animal feed, and particularly relates to application of tannin in preparation of drugs and/or functional products for eliminating displacement and/or recessive infection of salmonella in livers and lungs of poultry. The montanin disclosed by the invention can effectively inhibit displacement, colonization and recessive infection of salmonella in liver and lung of poultry, alleviate continuous injury of the liver and lung caused by the salmonella, reduce the recurrence rate after salmonella infection of the poultry, and improve the health level and growth performance of the poultry.

Mutagenesis method of high-yield DHA schizochytrium limacinum and application of high-yield DHA schizochytrium limacinum

Resumen de: CN119931841A

The invention discloses a mutagenesis method of high-yield DHA (docosahexaenoic acid) schizochytrium limacinum and application of the high-yield DHA schizochytrium limacinum, the high-yield DHA schizochytrium limacinum is obtained by taking schizochytrium limacinum as an original strain and utilizing ultraviolet mutagenesis, and the high-yield DHA schizochytrium limacinum is schizochytrium limacinum UV1-3. The schizochytrium limacinum mutant strain UV1-3 obtained through ultraviolet mutagenesis has the characteristic of high yield of DHA, the biomass, grease and DHA yield of the schizochytrium limacinum UV1-3 are remarkably improved, salmonella typhimurium can be inhibited, and weaned piglet salmonella infection inflammation can be relieved.

MYH7 gene related to resistance character of host salmonella pullorum as well as SNP (Single Nucleotide Polymorphism) molecular marker and application of MYH7 gene

Resumen de: CN119932201A

The invention relates to an MYH7 gene related to the resistance character of host salmonella pullorum, an SNP molecular marker and application of the MYH7 gene, and application of the MYH7 gene and the SNP molecular marker of the MYH7 gene in breeding or auxiliary breeding of chicken varieties with high resistance to salmonella pullorum. The nucleotide sequence of the MYH7 gene is as shown in SEQ ID NO.1; the MYH7 gene SNP molecular marker is located in an intron region of an MYH7 gene of a No.19 chromosome of a chicken, and the polymorphism is T or G at the 5853bp site of the No.19 chromosome; when the basic group at the SNP molecular marker of the MYH7 gene is T, the chicken has high resistance to salmonella pullorum; and when the basic group at the SNP molecular marker of the MYH7 gene is G, the resistance of the chicken to the salmonella pullorum is low. By utilizing the MYH7 gene and the SNP molecular marker thereof provided by the invention, a chicken variety with high resistance to salmonella pullorum can be quickly and effectively screened and bred.

Bioactive oligopeptide as well as extraction method and application thereof

Resumen de: CN119930536A

The invention provides a cyclic oligopeptide. The oligopeptide provided by the invention has an obvious inhibiting effect on lymphoma, leukemia, breast cancer, lung cancer, liver cancer or myeloma, has a certain inhibiting effect on escherichia coli, staphylococcus aureus, pseudomonas aeruginosa and salmonella paratyphi B, and also has a certain uric acid reducing effect. Therefore, the compound has great significance in preparation of antitumor drugs, antibacterial drugs and uric acid reducing drugs.

一种减毒鼠伤寒沙门氏菌EN-VNP菌株及其制备方法和应用

Nº publicación: CN119932072A 06/05/2025

Solicitante:

江苏靶标生物医药研究所有限公司

Resumen de: CN119932072A

本发明公开了一种减毒鼠伤寒沙门氏菌EN‑VNP菌株及其制备方法和应用，减毒鼠伤寒沙门氏菌EN‑VNP菌株的制备方法，包括如下步骤：在沙门氏菌VNP20009菌株基础上依次敲除或沉默鞭毛蛋白基因fliC、fljB、磷酸乙醇胺转移酶基因eptA、4‑氨基‑4‑脱氧‑L‑阿拉伯糖转移酶基因arnT、磷酸转移酶yeiU以及敲入或过表达磷酸转移酶基因lpxE之后形成的菌株，制得减毒鼠伤寒沙门氏菌EN‑VNP菌株。本发明的具有低毒和T细胞佐剂效果的减毒鼠伤寒沙门氏菌EN‑VNP及其制备的EN‑OMV抗原递送系统，使改善后的菌株不含有鞭毛蛋白且LPS分子结构发生改变，具有低毒和T细胞佐剂效果，且能够正常扩增培养。