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AQUATIC CULTIVATION SYSTEM, METHOD OF CULTIVATION AND PRODUCTS THEREFROM

Resumen de: WO2025191569A1

Provided by the present disclosure is an aquatic cultivation system (mic-RAS), method of cultivation and microalgae obtainable thereby. The system comprises an aquatic cultivating tank comprising treated media inlet and effluent outlet; a photo-membrane bioreactor (PMBR) comprising a membrane having selective permeability, a PMBR inlet and a PMBR outlet; and fluid communication lines for continuous circulating effluent from the effluent outlet into the PMBR and treated media from the PMBR outlet into the aquatic cultivating tank. The method comprises cultivating aquatic organisms in an aquatic cultivating media within a cultivating tank; cultivating microalgae in an effluent media within a PMBR, the PMBR comprising membrane having selective permeability, submerged within the effluent media, the cultivating of the microalgae comprises: illuminating the microalgae; and circulating effluent from the cultivating tank into the PMBR and treated media from the PMBR into the cultivating tank. Further there is provided a microalgae, preferably obtained or obtainable by the system and/or method.

Application of scenedesmus obliquus in improvement of saline-alkaline tolerance of suaeda glauca

Resumen de: CN120642695A

The invention discloses application of scenedesmus obliquus to improvement of saline-alkaline tolerance of suaeda glauca, and belongs to the technical field of saline-alkaline tolerance of suaeda glauca. The purpose of the invention is to improve the saline-alkali resistance of suaeda salsa and improve saline-alkali soil. The invention provides a method for improving saline-alkaline tolerance of suaeda glauca, which comprises the following steps: inoculating scenedesmus obliquus into a BG11 culture medium, culturing under the conditions of 30 DEG C and 200rpm oscillation, introducing 2.5% CO2 and giving 2000lux continuous illumination, after an algae solution reaches a logarithmic phase, centrifugally collecting algae under the condition of 5000rpm, washing the algae with distilled water for three times, and adding the algae into salt-containing wastewater until the final concentration is 0.05 g/L. The invention further provides a method for improving the saline-alkaline tolerance of suaeda glauca. The research reveals composition and functional differences of suaeda glauca rhizosphere bacterial communities under different irrigation treatments, and lays a foundation for promoting plant growth and improving saline-alkali soil by using microalgae.

Leachate treatment method based on microalgae coupling nitrification process

Resumen de: CN120647032A

The invention discloses a percolate treatment method based on a microalgae coupling nitration process, and belongs to the field of wastewater biological treatment and intelligent control, and the method comprises the following steps: constructing initial reaction conditions suitable for microalgae proliferation and nitration reaction; operating parameters such as ammonia nitrogen concentration, nitrite concentration, dissolved oxygen, temperature and microalgae concentration are collected to form real-time operating data; based on the weighted multi-parameter fitting relationship, calculating an optimal combination of the illumination intensity, the carbon source feeding rate, the cycle ratio and the reaction duration; when the ammonia nitrogen removal rate is continuously lower than a threshold value, calling historical data to carry out trend analysis, and dynamically correcting a fitting weight; applying the correction result to control the illumination condition and the water inlet and outlet proportion; after the operation cycle is finished, updating the fitting model by adopting an incremental learning mode to realize continuous optimization of the control strategy; according to the invention, the nitrogen element removal efficiency of the percolation liquid and the system stability are improved, and the self-adaptive adjustment capability is relatively strong.

Aquaculture tail water treatment method

Resumen de: CN120647067A

The invention belongs to the field of aquaculture tail water treatment, and provides an aquaculture tail water treatment method which comprises the following steps: S1, settling and intercepting tail water through the front end of an ecological ditch; s2, performing multi-stage purification treatment on the passing tail water through a sedimentation tank, a filter dam I, an aeration tank, a filter dam II, a microalgae membrane tank and an ecological purification tank. Membrane pool medium filtration is adopted, the manufacturing cost is low, popularization is easy, the ecological property is good, and treatment is more efficient; the tail water treatment method has the advantages of saving land occupation, guaranteeing up-to-standard discharge, and aiming at the defects of low nitrogen and phosphorus pollutant treatment efficiency and slow nitrogen and phosphorus purification speed in the prior art, the tail water treatment method can improve the treatment efficiency and guarantee up-to-standard discharge of the culture tail water.

Alkali precipitation harvesting and re-culture utilization process for microalgae

Resumen de: CN120648560A

The invention belongs to the technical field of bioengineering, and particularly relates to a microalgae alkali precipitation harvesting and re-culture utilization process which comprises the following steps: (1) adding an HEPES buffer solution into a microalgae culture solution to adjust the pH value of the microalgae culture solution to 7.5-8.0 to obtain a microalgae culture solution A; (2) adding a mixed solution of triethanolamine and sodium citrate into the microalgae culture solution A in the step (1) to adjust the pH value of the microalgae culture solution A to 8.0-9.0 to obtain a microalgae culture solution B; (3) adding an alkaline solution into the microalgae culture solution B in the step (2) to adjust the pH value of the microalgae culture solution B to 10.2-13.0 to obtain a microalgae culture solution C; and (4) standing and layering the microalgae culture solution C, discarding the supernatant, collecting the lower concentrated algae solution, transferring into the culture medium, and re-culturing. According to the microalgae alkali precipitation harvesting and re-culture utilization process, the microalgae culture process can be efficiently carried out, and the microalgae alkali precipitation harvesting and re-culture utilization process is suitable for various culture modes of autotrophy, heterotrophy and heterotrophy-autotrophy.

Wool washing wastewater recovery treatment process

Resumen de: CN120647049A

The invention relates to the technical field of environmental engineering, and discloses a wool washing wastewater recovery treatment process which comprises the following steps: S1, carrying out demulsification pretreatment on wastewater through bionic nano-enzyme and recovering wool fat, S2, carrying out supercritical water gasification reaction on the pretreated wastewater, synchronously decomposing organic matters and generating hydrogen, the method comprises the following steps: S1, gasifying effluent, S2, carrying out gasification on the effluent, S3, carrying out filtration and biodegradation on the gasified effluent by adopting a self-energy-supply dynamic membrane bioreactor, S4, carrying out deep purification on membrane effluent through a photocatalysis-microalgae coupling system, and S5, coordinating operation of the whole system through an intelligent control algorithm based on real-time monitoring data. According to the method disclosed by the invention, the technical effects of efficiently recovering the wool fat and improving the oil-water separation selectivity are achieved by adopting a technical scheme of synergistically enriching and demulsifying the magnetic response bionic nano-enzyme and the high-gradient magnetic field; the problems of high reaction residue, serious secondary pollution and easy enzyme inactivation are solved.

Preparation method of cross-linked polyglutamic acid and application of cross-linked polyglutamic acid in microalgae harvesting

Resumen de: CN120623519A

The invention belongs to the field of microbial fermentation and biopolymer modification, and particularly relates to a preparation method of cross-linked polyglutamic acid and application of the cross-linked polyglutamic acid in microalgae harvesting. The preparation method comprises the following steps: by taking polyglutamic acid fermentation liquor subjected to sterilization, salting-out and redissolving treatment or a solution prepared from polyglutamic acid powder as a raw material, adjusting the pH value to be within a proper range, adding ethanol into the polyglutamic acid fermentation liquor or the solution prepared from the polyglutamic acid powder in proportion, uniformly stirring, collecting pasty precipitate, and weighing; and adding a cross-linking agent into the paste precipitate according to a proper mass ratio of the cross-linking agent to the polyglutamic acid, uniformly mixing, spreading in a drying tray, drying in vacuum drying equipment at a certain temperature to constant weight, crushing by using a crusher, and sieving by using a 40-mesh sieve to obtain a cross-linked polyglutamic acid product. The invention provides another preparation method of the cross-linked polyglutamic acid, the cross-linking reaction and the drying process are fused, the ethanol is used as an accelerant of the reaction, the dosage of the cross-linking agent is small, the product yield is high, the preparation cost is low, and the preparation method is suitable for large-scale pr

Multifunctional microalgae hydrogel as well as preparation method and application thereof

Resumen de: CN120617146A

The invention belongs to the technical field of drug delivery systems, and discloses multifunctional microalgae hydrogel as well as a preparation method and application thereof. According to the multifunctional microalgae hydrogel, natural components are taken as carriers, efficient release of drugs at the far end of the intestinal tract is achieved through pH and an intestinal flora enzyme response mechanism, the retention time of the far end of the intestinal tract is remarkably prolonged in combination with the adhesion characteristic, and the time-space distribution of the drugs is monitored in real time by supporting a fluorescent tracing technology. Meanwhile, by precisely regulating and controlling the homeostasis of intestinal flora, the intestinal barrier and the blood brain barrier are repaired, neuroinflammation, neuronal apoptosis and A beta deposition are remarkably improved, and cognitive impairment is reversed. The technical bottlenecks of poor targeting property and low bioavailability of a traditional oral preparation are broken through, a safe and efficient novel drug delivery strategy is provided for intervening neurodegenerative diseases based on the intestine-brain axis, and the preparation has remarkable clinical transformation potential and application value.

Isochrysis galbana continuous culture system and continuous culture method

Resumen de: CN120624177A

The invention relates to the technical field of microalgae continuous culture, and particularly discloses an isochrysis galbana continuous culture system and a continuous culture method.The culture system comprises an upper computer and a lower computer; the upper computer is a remote monitoring and control platform based on a mobile terminal, is connected with the lower computer and is used for real-time monitoring, parameter setting and remote regulation and control; the lower computer comprises a pipeline type photo-biological reaction circulating unit, a control unit, a supplementary material adding unit and a discharged liquid collecting unit; based on the culture system, efficient, stable and low-cost continuous culture of isochrysis galbana can be realized.

Vegetable protein composition and production method thereof

Resumen de: CN120616038A

The invention provides a vegetable protein composition and a production method thereof, and belongs to the technical field of protein. Comprising the following steps: S1, removing bean substance resistance factors; s2, carrying out mixed fermentation on the microalgae, bean substances and protein mulberry, and separating to obtain protein peptide; s3, chelating metal ions of the protein peptide; s4, condensing the protein peptide chelated with the metal ions; s5, preparation of carboxylated pullulan polysaccharide; s6, preparation of hydrophobic modified carboxylated pullulan polysaccharide; and S7, preparing the plant protein composition. The prepared plant protein composition has good physiological activity, through the embedding technology, the storage stability, heat resistance, acid resistance and moisture resistance of protein peptide are improved, growth and development of livestock are promoted, the meat quality is improved, and the plant protein composition has wide application prospects.

Nano-engineered microalgae for photoimmunotherapy of melanoma and preparation method of nano-engineered microalgae

Resumen de: CN120617329A

The invention discloses nano-engineered microalgae for photoimmunotherapy of melanoma and a preparation method of the nano-engineered microalgae. The preparation method comprises the following steps: S1, respectively putting tannic acid, potassium carbonate, sodium citrate and HAuCl4. 3H2O into water to prepare a tannic acid solution with the concentration of 4.3 mg/mL, a potassium carbonate solution with the concentration of 20.7 mg/mL, a sodium citrate reduction solution with the concentration of 0.7 mg/mL and a HAuCl4 solution with the concentration of 10.0 mg/mL; s2, dissolving a tannic acid solution and a potassium carbonate solution in the sodium citrate reduction solution to obtain a mixed solution; s3, the mixed solution is heated under stirring, a HAuCl4 solution is dropwise added, stirring continues, and AuNP is obtained; and S4, mixing the AuNP obtained in the step S3 with the microalgae PCC 7942, standing, incubating, and centrifugally washing, so as to obtain the nano-engineered microalgae PCC-coated AuNP. The functionalized microalgae drug delivery system (PCC-coated AuNP) prepared by the invention has the performance of respectively generating O2 and H2 through photosynthesis and photocatalytic reaction under the irradiation of 635 nm red light.

Microalgae semi-artificial photosynthesis hydrogen production system as well as preparation method and application thereof

Resumen de: CN120624563A

The invention relates to the field of hydrogen production through semi-artificial photosynthesis, and discloses a microalgae semi-artificial photosynthesis hydrogen production system as well as a preparation method and application thereof. The microalgae semi-artificial photosynthesis hydrogen production system comprises a microalgae-cadmium-based chalcogenide semiconductor heterogeneous aggregate; the microalgae-cadmium-based chalcogenide semiconductor heterogeneous aggregate is composed of a three-dimensional multicellular structure and a cadmium-based chalcogenide semiconductor layered structure, wherein the three-dimensional multicellular structure is formed by mutual adhesion and agglomeration of internal microalgae cells, the cadmium-based chalcogenide semiconductor layered structure is formed on the outer surface of microalgae in situ, and a cadmium-based chalcogenide semiconductor is obtained by in-situ generation of cadmium ions and chalcogenide anions on the surface of the microalgae. According to the microalgae-cadmium-based chalcogenide semiconductor heterogeneous aggregate semi-artificial photosynthesis hydrogen production system provided by the invention, the problem that the hydrogen production time and the hydrogen production rate of a conventional microalgae aggregate photosynthesis hydrogen production system are difficult to consider at the same time is solved through endogenous oxygen inhibition and exogenous photo-induced electron synergy; and the developm

Microalgae culture medium and microalgae culture method

Resumen de: CN120624206A

The invention provides a microalgae culture medium and a microalgae culture method, and belongs to the technical field of algae culture, the microalgae culture medium provided by the invention comprises a nitrogen source and a salt agent, the mass ratio of the nitrogen source to the salt agent is 1: (0.7-1.3), and the nitrogen source comprises a phytic acid compound, amino acid and putrescine. When the microalgae culture medium provided by the invention is used for culturing microalgae, the growth rate, the total lipid accumulation rate and the unsaturated fatty acid content of the microalgae can be effectively improved, and the microalgae culture medium provided by the invention is not easy to cause eutrophication of a water body and is suitable for large-scale culture of the microalgae.

Microalgae culture method

Resumen de: CN120607967A

The invention relates to a microalgae culture method which comprises the following steps: microalgae species are cultured in the presence of a culture solution, a light source for culture is a flash light source, the light emitting duration of the flash light source each time is 0.1-2.0 ms, and the non-light emitting duration of the flash light source is 0.2-4.5 ms. The flash light source is adopted as a culture light source of microalgae, the illumination power consumption for producing unit microalgae biomass can be effectively reduced, the light energy utilization rate and the microalgae biomass production efficiency are improved, and good economic benefits are achieved.

Biological carbon sequestration reaction system

Resumen de: CN223316676U

The utility model relates to the technical field of biological carbon sequestration, in particular to a biological carbon sequestration reaction system, which comprises a micro-nano bubble aeration device used for generating a micro-nano bubble solution and conveying the micro-nano bubble solution into a mixing tank; the mixing tank is used for mixing the micro-nano bubble solution and the microalgae solution entering the mixing tank and then conveying the mixed solution into the buffer column; and the buffer column comprises circulating equipment which is used for circularly conveying the mixed liquid at the bottom of the buffer column into the buffer column through a reactor pipeline. By applying the biological carbon sequestration reaction system, a micro-nano bubble solution can be generated through the micro-nano bubble aeration device, a microalgae solution of the micro-nano bubble solution entering the mixing tank can be mixed through the mixing tank, and the mixed solution is conveyed into the buffer column; the mixed liquid at the bottom of the buffer column can be circularly conveyed through a reactor pipeline by virtue of circulating equipment, so that in practical application, the photosynthesis and respiration of microalgae can be effectively promoted, and the activity and growth speed of the microalgae are enhanced.

Blue-green algae large-scale culture system

Resumen de: CN223316661U

The utility model provides a large-scale blue-green algae culture system. The system comprises a culture pond unit, a supernatant storage unit and a blue-green algae liquid storage unit, the culture pond unit is provided with a plurality of blue-green algae culture ponds, each blue-green algae culture pond is provided with a double-drainage mechanism, and the blue-green algae culture ponds are communicated with the supernatant storage unit and the blue-green algae liquid storage unit through the double-drainage mechanisms; the double-drainage mechanism comprises a first pipeline and a second pipeline, a first liquid outlet is formed in the bottom of the blue-green algae culture pond, a second liquid outlet is formed in the position, away from the ground by a selected height, of the pond body, the first pipeline is communicated with the first liquid outlet, and the second pipeline is communicated with the second liquid outlet; the supernate storage unit is provided with a third pipeline and a first liquid conveying mechanism, the third pipeline is communicated with the blue-green algae culture pond, and the liquid conveying mechanism is used for conveying supernate in the supernate storage unit into the blue-green algae culture pond through the third pipeline. The system can realize separation of cyanobacteria liquid and supernate in the culture pond and cyclic utilization of the supernate.

Method for improving stress tolerance of microalgae endocrine disrupter by utilizing phycobacterium

Resumen de: CN120607984A

The invention relates to the field of microorganisms, and discloses a method for improving the stress tolerance of microalgae endocrine disruptors by utilizing phycobacterium, which comprises the following steps: co-culturing phycobacterium and microalgae; the endocrine disrupter is nonyl phenol; the phycobacterium is rosette-shaped rhizobium and/or brevundimonas vesicula. According to the invention, the effect of improving the stress tolerance of the endocrine disrupter (nonyl phenol) of the microalgae by part of interalgal bacteria of the microalgae is found for the first time. By co-culturing the algae and the microalgae, the survival and growth capabilities of the microalgae under the stress of nonyl phenol can be effectively improved, so that the tolerance and removal capability of the microalgae to high-concentration endocrine disrupter polluted wastewater are expected to be improved in an algae-bacteria co-culture manner.

Method of producing a medium for culturing cyanobacteria

Resumen de: PL447926A1

Przedmiotem zgłoszenia jest sposób wytwarzania pożywki do hodowli sinic z biomasy bakteryjnej po produkcji polihydroksyalkanianów. Wynalazek znajduje zastosowanie w hodowli zwłaszcza Arthrospira platensis.

Chlorella and application thereof in carbon sequestration, emission reduction and biological fertilizer co-production

Resumen de: CN120591103A

The invention discloses chlorella and application thereof in carbon sequestration and emission reduction and co-production of a biological fertilizer, and a process for coupling carbon sequestration and emission reduction of microalgae and utilization of a microalgae biological fertilizer in flue gas of a power plant comprises the following steps: 1) culturing an algal strain in the flue gas of the power plant, and 2) harvesting the algal strain cultured in the step 1) and using the algal strain to prepare the microalgae biological fertilizer. Through experiments, aiming at the defects of the traditional flue gas treatment technology of the coal-fired power plant, the invention adopts a microalgae carbon sequestration technology which is environment-friendly, economical and efficient and conforms to the natural circulation characteristics, and is coupled with a microalgae biological fertilizer, so that the flue gas treatment is realized, the flue gas resource utilization is realized, the whole ecological chain is prolonged, and the environmental protection is realized. And the full-life-cycle carbon sequestration emission reduction amount is effectively increased.

Preparation method of microalgae bait

Resumen de: CN120584976A

The invention provides a preparation method of microalgae bait, the microalgae bait is prepared through microalgae culture, magnetic separation, microencapsulation wrapping, drying and other methods, during culture, a specific culture solution is added, culture conditions are adjusted, algae liquid cells are separated by adopting a nano magnetic dispersant, effective components in the microalgae cells are ensured, mixed cell sap is screened out, and the microalgae bait is prepared. The components in the core material are wrapped through microencapsulation wrapping, the components of the wall material are used for isolating the external environment, good oxidation resistance and water resistance are achieved, notches and pores of the bait are reduced after wrapping, the performance is stable, the storage life is long, the swelling and collapsing time in water is prolonged, collapsing of nutritional ingredients in the core material is delayed, and the bait has the advantages of being good in stability and good in stability. The loss rate is low, the acid value change rate is within 2.20%, the peroxide value change rate is within 1.41%, the water resistance time reaches 8.5 h, the loss rate is within 4.85%, the sinking rate reaches 98%, the bait is easy to sink into the water bottom and beneficial to being ingested by aquatic animals, and the utilization rate of the bait is increased.

Efficient microalgae culture equipment and method

Resumen de: CN120591067A

The invention discloses efficient microalgae culture equipment which comprises a raceway pond structure, a concentric table, an electric belt conveying mechanism, a water stirring lifting mechanism and a circulating ventilation mechanism, a pond body is arranged in the raceway pond structure, the concentric table is arranged in the center of the pond body, and the electric belt conveying mechanism is installed on the concentric table and drives the circulating ventilation mechanism to move along a set track; the water-stirring lifting mechanism is arranged on one side of the tank body and comprises a screw rod for adjusting the height, a lifting block, a sealing sliding plate and a water-stirring impeller, the circulating ventilation mechanism comprises an air compression mechanism, a ventilation pipe, a transverse pipe and an air outlet head, and the air compression mechanism compresses air through a piston assembly and injects the air into the bottom of the tank body through the air outlet head; according to the equipment, the height of the water-repellent impeller is automatically adjusted along with the liquid level through the water-repellent lifting mechanism, the movable air outlet structure is combined to expand the inflation range and prevent blockage, the air compression mechanism realizes efficient one-way air supply through mechanical reciprocating, and the ventilation effect is improved.

Microalgae species storage rack

Resumen de: CN223304431U

The utility model provides a microalgae species preservation rack. The microalgae species preservation rack comprises a main frame, a containing box, a lighting device and an inflation device. The containing box is installed on the main frame, a containing cavity used for containing a flask is formed in the containing box, and an opening communicated with the containing cavity is formed in the front side of the containing box; the illumination device is mounted on the accommodating box and comprises a plurality of lamp tubes and a switch for controlling the lamp tubes, and the lamp tubes are mounted at the top of the accommodating cavity and provide illumination for microalgae species in the flask in the accommodating cavity; and the air charging device is mounted on the main frame and is used for supplying air to microalgae species in the flasks in the accommodating cavity. The storage rack can ensure the stability and the reliability of a microalgae cultivation environment, and the cultivation protection quality of microalgae is improved.

LED lamp applied to microalgae cultivation

Resumen de: CN223306764U

The utility model relates to the technical field of lighting lamps, in particular to an LED lamp applied to microalgae cultivation, which is characterized by comprising a lamp housing, a lamp panel and a lens, the lamp panel is mounted in the lamp housing, a light source for emitting light is mounted on the lamp panel, and the lens is mounted at an opening of the lamp housing and shields the lamp panel. Therefore, the light emitted by the light source is diffused, and the irradiation range of the light is expanded. The utility model has the advantages that the illumination uniformity is improved, and the exposure is reduced.

Lignocellulose pretreatment liquid resource utilization method based on algal-bacterial co-culture

Resumen de: CN120589942A

The invention discloses a lignocellulose pretreatment liquid resource utilization method based on bacterium-alga co-culture, which comprises the following steps: by taking Kurthia huakuii bacteria and Chlorella sorokiniana microalgae as model microorganisms, establishing an alga-bacterium symbiotic system, inoculating the alga-bacterium symbiotic system into a lignocellulose pretreatment liquid, carrying out phototrophic growth and nitrogen assimilation on the Kurthia huakuii bacteria rapid detoxification inhibitor and the Chlorella sorokiniana microalgae by using bacterial metabolites (organic acid and CO2), and carrying out anaerobic fermentation and nitrogen assimilation on the lignocellulose pretreatment liquid, so as to obtain the lignocellulose pretreatment liquid resource utilization method based on bacterium-alga co-culture. According to the method, toxic substances in lignocellulose pretreatment can be efficiently detoxified, organic carbon in lignocellulose pretreatment liquid is recycled and converted into high-value phycomycete biomass, and the utilization rate of carbon and nitrogen is increased.

GLASS NOODLES WASTEWATER TREATMENT PROCESS BASED ON MICROALGAE

Nº publicación: AU2024387147A1 04/09/2025

Solicitante:

SHANDONG ACAD OF AGRICULTURAL SCIENCES
SHANDONG ACADEMY OF AGRICULTURAL SCIENCES

Resumen de: AU2024387147A1

The present invention relates to a glass noodles wastewater treatment process based on microalgae, belonging to the technical field of microbial wastewater treatment. Chlorella sorokiniana NZ1 and Monoraphidium sp. NZ4 are selected. An analysis of experiment results shows that when Chlorella sorokiniana NZ1 and Monoraphidium sp. NZ4 are subjected to mixed culturing, the biomass of both in the wastewater reaches the highest level. At the same time, the removal rates of water quality indicators also exhibit the most significant improvement; specifically, the removal rates of COD, TN, ammonia nitrogen, and TP can respectively reach 83.4%, 91.19%, 81.51%, and 78.71%. Thus, it can be concluded that subjecting the algal strains NZ1 and NZ4 to mixed culturing is the most effective glass noodles wastewater treatment method.