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69
resultados
Última actualización
14/09/2026 [08:19:00]
Resultados 25 a 50 de 69
Resumen de: CN122278855A
The invention discloses sgRNA (small guide RNA), an RPA (recombinase polymerase amplification) primer pair and application of the sgRNA and the RPA primer pair in campylobacter coli detection. The sgRNA contains a target sequence of which the nucleotide sequence is as shown in SEQ ID NO.1 or SEQ ID NO.2. The lowest detection limit of the detection method is 100 CFU/mL, the specificity is high, the detection operation is simple, the detection method is not limited by site equipment and professional ability of operators, the application range is wide, the detection method can be used for rapidly detecting campylobacter colons in actual excrement samples, and the detection result is accurate.
Resumen de: CN122282714A
The invention discloses an embedded whole-cell biosensor, a preparation method of the embedded whole-cell biosensor and application of the embedded whole-cell biosensor in machine learning assisted mercury ion detection, and belongs to the technical field of sensor materials and detection. According to the sensor, escherichia coli (E. coli) for expressing green fluorescent protein (GFP) is encapsulated in a polyacrylamide/hyaluronic acid (PAAM/HA) hydrogel matrix, so that the sensor can realize high-selectivity and high-sensitivity detection on mercury ions, can effectively eliminate interference of competitive metal ions, can realize complete regeneration through EDTA-2Na treatment, and can be used for detecting mercury ions in a high-sensitivity manner. The device can be repeatedly used under the condition that the performance is not lost; stable fluorescence response as long as three weeks can be maintained; excellent biocompatibility and biodegradability are achieved, and the risk of secondary pollution is reduced to the maximum extent; and rapid on-site analysis is facilitated.
Resumen de: CN122256540A
The invention provides a molecular diagnosis method combining a multi-enzyme isothermal rapid amplification (MIRA) technology and a CRISPR-Cas12a nucleic acid detection technology, and particularly relates to application of the molecular diagnosis method in rapid, sensitive and specific detection of common pathogenic bacteria infected by a central nervous system (CNS). By combining a multi-enzyme isothermal rapid amplification technology with a CRISPR-Cas12a nucleic acid detection system, rapid, sensitive and specific detection of nucleic acid of common pathogenic bacteria of the central nervous system is realized under a constant temperature condition, so that a new technical means is provided for early diagnosis of central nervous system infection.
Resumen de: CN122256541A
The invention provides a kit for detecting common pathogenic bacteria of otitis media based on a multi-enzyme constant-temperature rapid nucleic acid amplification technology. The kit comprises a first primer pair for detecting streptococcus pneumoniae, a second primer pair for detecting haemophilus influenzae, a third primer pair for detecting moraxella catarrhalis, a fluorescent probe combination and a colloidal gold probe combination, the nucleotide sequences of the first primer pair are respectively SEQ ID NO: 2 and SEQ ID NO: 4, the nucleotide sequences of the second primer pair are respectively SEQ ID NO: 9 and SEQ ID NO: 10, and the nucleotide sequences of the third primer pair are respectively SEQ ID NO: 13 and SEQ ID NO: 18. According to the triple multienzyme constant-temperature rapid synchronous detection kit and the detection method, high sensitivity, high specificity and operation convenience are achieved, and the clinical bedside rapid diagnosis requirement is met.
Resumen de: CN122235342A
The invention relates to the technical field of biological detection, and particularly discloses a gene chip for simultaneously detecting 12 food-borne pathogenic bacteria and application thereof, the gene chip comprises a polyvinyl chloride solid-phase carrier, and a specific probe, a positive control probe and a hybridization control probe which are fixed on the surface of the polyvinyl chloride solid-phase carrier. The gene chip can be used for simultaneously detecting salmonella, escherichia coli O157: H7, staphylococcus aureus, listeria monocytogenes, bacillus cereus, vibrio parahaemolyticus, vibrio cholerae, vibrio fluvialis, shigella flexneri, campylobacter jejuni, clostridium perfringens and yersinia enterocolitica. The gene chip disclosed by the invention is arranged by adopting a preset dot matrix, and detection is realized through sample total DNA extraction, multiple PCR amplification and biotin labeling, chip hybridization, washing, sealing, enzyme labeling, color development and scanning interpretation; the method can realize synchronous detection of 12 target bacteria, and has good specificity, repeatability and application value.
Resumen de: US20260168026A1
The present invention relates to a CRISPR-Cas-based composition for detection of Listeria monocytogenes and a Listeria monocytogenes detection method using same and, more specifically, to a composition for detection of Listeria monocytogenes, comprising a primer pair capable of specifically amplifying Listeria monocytogenes by isothermal amplification, a guide RNA, and a CRISPR-Cas protein, and a detection method using same.
Resumen de: CN122214517A
The invention relates to a triple multiple fluorescence detection kit based on a synergistic pathogenic mechanism of enteropathogenic bacteria and application of the triple multiple fluorescence detection kit. The kit comprises a sample treatment solution, targeted capture magnetic beads, an eluent, a multiple PCR premixed solution, a primer probe mixed solution, a positive reference substance, a negative reference substance and an exogenous internal standard, the sequences of the primer probes are as shown in SEQ ID NO. 1-9. The invention also provides a method for detecting enterotoxin-producing bacteroides fragilis, escherichia coli carrying pks gene island and fusobacterium nucleatum in excrement by using the kit, and a method for assisting in evaluating the risk of colorectal cancer. The triple multiple fluorescence detection kit breaks through the technical bottleneck of synchronous and accurate detection of high and low abundance targets in multiple fluorescence PCR, ensures the detection sensitivity of low abundance key targets BFT gene and clbB gene, significantly reduces the false negative risk, and provides a more comprehensive molecular basis for early warning and risk stratification.
Resumen de: CN122211684A
The invention belongs to the technical field of food safety detection, and discloses a self-locking kit for nucleic acid detection of food-borne escherichia coli O157 and an operation method of the self-locking kit. The self-locking kit comprises a self-locking unit consisting of a clamping arm, a guide rail, an elastic element and a lock catch, when the reagent tube is not put in, the lock catch limits the clamping arm, and when the reagent tube is put in, the guide rail and the elastic element are matched with the clamping arm, so that when the buckle end of the lock catch is separated from the limiting groove, the elastic element extends to push the lower end of the clamping arm to slide outwards, and the clamping arm is clamped in the limiting groove. And the upper ends of the clamping arms are driven to fold inwards to clamp the reagent tube. The self-locking unit utilizes a lever linkage principle and a spring energy storage release mechanism to solve the contradiction between fixing and taking and placing convenience of the reagent tube.
Resumen de: CN122214522A
The invention discloses an LAMP-CRISPR primer composition, a kit and a detection method for detecting Escherichia coli, and belongs to the technical field of molecular detection. According to the invention, LAMP primers and a Cas12b-sgRNA specific recognition sequence are synergistically designed, an LAMP primer group with nucleotide sequences as shown in SEQ ID NO.1-6 and sgRNA as shown in SEQ ID NO.9 are provided, an LAMP-CRISPR primer composition is formed, and the LAMP-CRISPR primer composition and a CRISPR/Cas12b system are made into the kit. LAMP amplification and CRISPR/Cas12b are combined for use, a one-step constant-temperature reaction system is constructed, the amplification detection process is remarkably shortened, meanwhile, high specificity and high sensitivity are achieved, and the problems that a sepsis pathogenic bacterium detection technology is long in time consumption, poor in specificity, incapable of achieving multiple detection, difficult to apply beside a bed and the like are effectively solved.
Resumen de: CN122214285A
The invention discloses application of a salmonella bacteriophage vBSalDSP13 and a rapid detection method of the salmonella bacteriophage vBSalDSP13. The preservation number of the bacteriophage vBSalDSP13 is GDMCC No: 67876-B1, and the preservation number of the bacteriophage vBSalDSP13 The virulent bacteriophage vBSalDSP13 provided by the invention is wide in host spectrum, can split up to 25 salmonella serotypes, can split two strains of escherichia coli at the same time, and has a characteristic molecular target. The bacteriophage SP13 has good thermal stability, pH stability and chloroform tolerance, is not easy to inactivate in the application process, and has a good inhibition effect on target bacteria in milk and chicken. In conclusion, the bacteriophage SP13 can be used as a single inhibitor or combined with other pathogenic bacteria bacteriophages to form an inhibitor for preventing and controlling pathogenic bacteria such as salmonella and the like.
Resumen de: CN122189228A
The invention belongs to the technical field of molecular detection of plant diseases, and discloses a primer probe composition, a kit and an RPA-LFD detection method for detecting pathogenic bacteria of northern corn leaf blight, and the primer probe composition comprises an upstream primer as shown in SEQ ID NO.1, a downstream primer marked by 5 '-terminal biotin as shown in SEQ ID NO.2, and a primer probe. The probe is marked by a 5'end fluorophore as shown in SEQ ID NO.3, contains a THF site in the middle and is modified by a 3 'end blocking group. The invention also provides a kit containing the composition and an LFD test strip. The detection method comprises the following steps: extracting DNA of a sample, carrying out RPA amplification by adopting the primer probe composition, detecting an amplification product through an LFD test strip, and judging whether the amplification product contains target pathogenic bacteria or not according to a color development result. The method is high in specificity, high in sensitivity, simple, convenient and rapid to operate, visible in result by naked eyes, free of precise instruments and suitable for on-site rapid detection and early diagnosis of northern corn leaf blight pathogenic bacteria.
Resumen de: CN122189147A
The invention discloses a detection stirring rod based on co-modification of bacteriophage and beta-galactosidase as well as a preparation method and application of the detection stirring rod, belongs to the technical field of analysis and detection, and is mainly used for field detection of pseudomonas aeruginosa ATCC 15442 type. The detection stirring rod takes a wood fiber rod as a substrate, the surface of the substrate is modified with poly (diallyldimethylammonium chloride), and the poly (diallyldimethylammonium chloride) is co-modified with bacteriophage and beta-galactosidase; the bacteriophage is immobilized through dual effects of covalent binding and electrostatic adsorption, and the beta-galactosidase is immobilized through electrostatic adsorption. The stirring rod integrates enrichment-detection functions, the total detection time is less than or equal to 6min, the detection limit is as low as 4CFU/ml, the specificity is strong, no cross reaction exists, the cost is low, the operation is simple and convenient, the detection recovery rate of a real sample reaches 83%-118%, and the stirring rod is adaptive to the rapid detection requirements of multiple scenes.
Resumen de: CN122189209A
The invention relates to the fields of molecular diagnosis, synthetic biology and in-vitro detection, in particular to a molecular sensor system for directly detecting mycobacterium tuberculosis, which comprises a sequence-specific nucleic acid control module, a robust cell-free transcription and translation system and a detection substrate, the sequence-specific nucleic acid control module comprises a single-chain trigger recognition region designed for target RNA (Ribonucleic Acid) highly expressed by mycobacterium tuberculosis in a sputum microenvironment, and a signal report module regulated and controlled by the trigger recognition region; wherein the signal reporting module is used for coding NanoLuc luciferase; the robust cell-free transcription and translation system comprises a T7 RNA polymerase, an escherichia coli ribosome component, an RNase inhibitor with an effective concentration, and at least one matrix stable factor selected from trehalose and a nonionic surfactant. The detection substrate comprises a coelenterazine derivative which is suitable for the NanoLuc luciferase; therefore, the direct detection of the mycobacterium tuberculosis RNA in the sputum sample is realized.
Resumen de: CN122187691A
The invention relates to a D-alanine biological orthogonal probe platform, discloses a preparation method and application thereof, and belongs to the technical field of biological medicine. The probe A1 is firstly metabolized and doped into cell walls, the cell wall permeability of B1, B2 and C1 is gradually enhanced along with the gradual reduction of molecular weight, the fluorescent probe C1 and A1 are effectively subjected to site-click reaction so as to start fluorescence, and gram-positive bacteria staphylococcus aureus, bacillus subtilis and gram-negative bacteria escherichia coli are specifically detected. L-type bacteria losing cell walls are detected through induction of L-type transformation, and the L-type bacteria are obviously distinguished from normal bacteria containing the cell walls. The probe is also used for researching the anti-microbial drug resistance, and powerful evidence of the conclusion that the L-type bacteria cause the drug resistance of the bacteria is obtained. The research lays a foundation for developing the D-alanine biological orthogonal probe for detecting L-type bacteria and researching and diagnosing clinical pathogenic bacteria.
Resumen de: NL1044990A
0001 Abstract: The current invention comprises the novel technological development regarding the detection of fond related bacteria, such as listeria, e. coli and s. aureus in a mobile and modular design. The combination of a multiple target sensor, smart readout optics and spectral processing enables to instantly ascertain, verify and validate the presence of specific microbes. The main application is the monitoring of the fond and fond production in order to prevent the coming to the market of bacteria invested products, such as fish, chicken or other fond products destined for human consumption.
Resumen de: CN122189216A
The invention discloses a multiple fluorescent quantitative PCR (Polymerase Chain Reaction) detection method and a multiple fluorescent quantitative PCR detection kit capable of simultaneously detecting three microorganisms. The primer pair and probe combination comprises a specific primer pair and a specific probe aiming at escherichia coli, a specific primer pair and a specific probe aiming at pasteurella multocida, and a specific primer pair and a specific probe aiming at clostridium perfringens. According to the method, escherichia coli, pasteurella multocida and clostridium perfringens can be synchronously detected through a single tube, the detection period is greatly shortened, and the detection cost is reduced; the primer probe has excellent compatibility, is simple and convenient to operate, and is suitable for clinical diagnosis and treatment, epidemiological investigation and other multi-scene application.
Resumen de: CN122189217A
The invention relates to a composition of universal oligonucleotide primers and probes for detecting pseudomonas bacteria based on an ERA fluorescence method or an ERA test strip method and a detection method using the composition. The detection method disclosed by the invention can be used for rapidly, specifically and sensitively detecting whether the pseudomonas bacteria exist in the sample or not, is simple and convenient to operate and short in reaction time, does not need complex temperature control equipment, and is suitable for rapid screening, pollution monitoring and risk early warning of the pseudomonas bacteria in milk and dairy product samples, food samples, environmental samples, water samples and related monitoring samples.
Resumen de: CN122189218A
The invention belongs to the technical field of biological diagnosis and environmental detection, and particularly relates to an Escherichia coli detection system and kit based on RPA-Cas12a and application of the Escherichia coli detection system and kit. According to the detection system, a UidA gene is taken as a target sequence, a primer for RPA isothermal amplification is designed, and an RPA amplification product is visually displayed or detected by a microplate reader through an RPA-Cas12a technology, so that whether a sample to be detected contains escherichia coli or not is judged. Compared with a traditional culture detection method, the detection method disclosed by the invention has the advantages that the detection efficiency is remarkably improved, complicated equipment is not needed, and the method is suitable for on-site rapid detection; through real-time fluorescence signal monitoring, a detection result can be obtained in real time, and the operation convenience and efficiency are further improved; besides, the detection method can detect escherichia coli as low as 100 copies/L, the sensitivity is high, the detection period is short, and detection can be completed within 30 min.
Resumen de: CN122193573A
The invention discloses a kit for simultaneously detecting two food-borne pathogenic bacteria based on an SERS (Surface Enhanced Raman Scattering) technology as well as a preparation method and application of the kit, synchronous detection of double target bacteria is realized, staphylococcus aureus and escherichia coli O157: H7 can be simultaneously recognized and quantified in single detection through an SERS probe modified by two specific aptamers, and the detection efficiency is remarkably improved. The etSi (at) ZnO-Ag-Au composite substrate constructed by the method disclosed by the invention is strong in anti-interference capability and good in reproducibility. The method is high in sensitivity and low in detection limit. The sample pretreatment is simple, the detection steps are convenient and fast, the portable Raman spectrometer is combined, the detection can be quickly completed on the drug production and storage and transportation site, and the requirement on the professional skill of an operator is low.
Resumen de: WO2026121311A1
The present invention provides: a method for detecting an antibody against colibactin-producing bacteria in a subject; and a reagent or kit for detecting an antibody against colibactin-producing bacteria in a subject. Provided is a method for detecting an anti-colibactin-producing bacteria antibody in a sample, said method including a step for bringing the sample into contact with at least one of the lipopolysaccharides from among O2, O4, O6, O18, and O50 serotypes of Escherichia coli. Provided is a reagent or kit for detecting an anti-colibactin-producing bacteria antibody, said reagent or kit containing a sample and at least one of the lipopolysaccharides from among O2, O4, O6, O18, and O50 serotypes of Escherichia coli.
Resumen de: CN122168716A
The invention discloses a traditional Chinese medicine bacteriostatic activity detection process for microbiological detection, and relates to the technical field of traditional Chinese medicine activity detection. The process comprises four core steps of sample pretreatment, bacterial liquid preparation, bacteriostatic activity detection, result judgment and data analysis, and solves the technical problems of low sensitivity, poor repeatability and inaccurate result of a traditional detection method by optimizing an extraction solvent ratio, setting gradient concentration, culturing conditions and performing multi-index combined judgment. The standard operation process is adopted, the bacteriostatic activity of various traditional Chinese medicine samples such as traditional Chinese medicine extracts and compound preparations can be accurately detected, the method is suitable for common pathogenic bacteria such as escherichia coli and staphylococcus aureus and fungi such as candida albicans, the detection result is accurate and reliable, repeatability is good, and the method is suitable for popularization and application. The invention provides a scientific and efficient detection method for bacteriostatic activity evaluation, quality control and new drug research and development of traditional Chinese medicines, and has a wide application prospect.
Resumen de: CN122168784A
The invention belongs to the technical field of biological detection, and particularly relates to an RPA primer and a kit for detecting escherichia coli and application of the RPA primer and the kit. The target sequence is a broad-spectrum and specific new target DNA sequence in Escherichia coli, and the RPA primer designed by the target sequence can be used for a recombinase polymerase isothermal amplification reaction of Escherichia coli. On the basis, the invention provides an RPA detection method for detecting the escherichia coli by using the RPA primer, and compared with the existing PCR detection technology, the isothermal amplification detection method disclosed by the invention can be used for rapidly detecting the escherichia coli at normal temperature or under slight heating, the detection can be completed within 30 minutes, and the detection efficiency is remarkably improved; moreover, the detection method disclosed by the invention is relatively high in specificity, the detection sensitivity of the detection method can detect escherichia coli as low as 101 copies/L, and the specificity and the sensitivity are far higher than those of a traditional PCR (Polymerase Chain Reaction) detection technology.
Resumen de: CN122168779A
The invention discloses a kit for rapidly detecting HAP/VAP pathogenic bacteria and drug-resistant genes and application of the kit, and belongs to the technical field of biomedical detection. The invention provides an optimized detection kit aiming at the problems of long detection process, complex operation and poor sample adaptability of the existing tNGS technology. The kit comprises a sample pretreatment and nucleic acid extraction reagent system, a sample preservation solution of which contains 5% of dithiothreitol and 2.5% of potassium azide and is used for synchronously completing sample liquefaction and salt ion chelation in the transportation process; the amplification library-building reagent system comprises a primer mixture consisting of 175 pairs of primers, escherichia coli synthetase III and independently added magnesium ions. By integrating the reagent system and optimizing the whole-process operation, the whole-process time from sample pretreatment to report issuing is shortened to be within 2.5 hours. The method has the advantages of being extremely high in detection speed, wide in sample applicability, high in operation stability and easy to realize automation.
Resumen de: CN122171800A
The invention belongs to the technical field of biological detection, and provides a biosensor for detecting salmonella typhimurium based on CRISPR/Cas12a activated by a DNA nanowire amplified by DNAzyme, and the biosensor comprises CRISPR/Cas12a protein, Mg < 2 + >, an aptamer Apt with a nucleotide sequence as shown in SEQ ID NO: 1-8, a chain A, a chain AB, a DNAzyme chain, a chain H1, a chain H2, a chain H3 and crRNA. The biosensor is high in detection sensitivity, low in detection limit, high in specificity, small in background interference, stable in fluorescence signal, simple to operate, mild in reaction condition, suitable for on-site rapid detection and suitable for actual food sample detection, the detection range can reach 10 <-10 > cfu/mL order of magnitude, the adding standard recovery rate is 100.3%-103.9%, and the detection cost is low. The method can be widely applied to salmonella typhimurium detection in the fields of food industry and public health.
Nº publicación: CN122150572A 05/06/2026
Solicitante:
NORTHEAST NORMAL UNIV
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Resumen de: CN122150572A
The invention discloses an independent electrochemical immunosensing device for instantly detecting salmonella typhimurium. The invention firstly provides a bendable patch. The bendable patch comprises a microfluidic module and a sensing module, the sensing module comprises a substrate and an immune sensing layer arranged on the substrate, and a working electrode, a reference electrode and a counter electrode are arranged on the immune sensing layer; the working electrode is obtained by sequentially modifying a carbon electrode with 3D-PCNAs, EDC/NHS, a salmonella typhimurium antibody and bovine serum albumin; the microfluidic module includes a sealing layer, a spacer layer, and an automatic transport layer. The free-standing electrochemical immunosensing device provided by the invention comprises a bendable patch and an electronic module. The electrochemical immunosensing device is independent working equipment, has the characteristics of no need of a peristaltic pump and low cost, and is suitable for on-site instant detection of salmonella typhimurium.
BOPI
Sede Electrónica
