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C8 esterase activated double-lock chemiluminescent probe, preparation method thereof and application of C8 esterase activated double-lock chemiluminescent probe in preparation of detection kit for detecting salmonella

Resumen de: CN121270430A

The invention relates to the technical field of chemical analysis and detection, and discloses a C8 esterase activated double-lock chemiluminescence probe, a preparation method thereof and application of the C8 esterase activated double-lock chemiluminescence probe in preparation of a salmonella detection kit, the double-lock chemiluminescence probe is DSCP-CBCN, and the structural formula of the double-lock chemiluminescence probe is shown in the specification. The DSCP-CBCN probe is composed of a C8 esterase cleavable substrate, a phenoxy cyclobutane chemiluminophor and a self-eliminating connexon p-hydroxy benzyl alcohol. After the DSCP-CBCN probe and salmonella are incubated, sodium hypochlorite and hydrogen peroxide are added to generate remarkable chemiluminescence, POCT rapid detection of salmonella can be realized by combining a chemiluminescence microplate reader, the bacterial culture time is shortened to 3-7 hours, the limit of detection (LOD) value is as low as 1.9 CFU/mL, and the sensitivity is improved by 1008 times compared with that of a commercial MUCAP probe. The method is suitable for salmonella large-scale screening in the fields of clinical pathogen diagnosis, food safety detection and environmental health monitoring.

Kit for rapidly and jointly detecting multiple pathogen nucleic acids related to dyspnea pregnancy outcome and application of kit

Nº publicación: CN121272078A 06/01/2026

Solicitante:

PEKING UNIV THIRD HOSPITAL PEKING UNIV THIRD CLINICAL MEDICAL COLLEGE
BEIJING COYOTE BIOSCIENCE CO LTD
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Resumen de: CN121272078A

The invention belongs to the technical field of in-vitro diagnostic reagents for rapid detection of human pathogenic microorganisms, and particularly relates to a kit for rapid combined detection of multiple pathogen nucleic acids related to a dyspnea pregnancy outcome and application of the kit. The pathogen is selected from one or more of escherichia coli, candida albicans, candida glabrata, group B streptococcus, streptococcus gonorrhoeae, chlamydia trachomatis or ureaplasma urealyticum, and the kit comprises a pathogen and lactobacillus rapid detection micro-fluidic chip. A primer group for detecting the pathogen and the lactic acid bacillus nucleic acid and an array consisting of an amplification control and a blank control are fixed on the micro-fluidic chip. According to the present invention, the microfluidic chip rapid detection technology and the nucleic acid amplification technology are adopted to achieve the purposes of rapid detection and multiple detection so as to rapidly and conveniently determine the poor prognosis risk of the pregnant woman at present, timely take the specific measures, and avoid the occurrence of the poor prognosis.