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Method for simultaneously detecting six pathogenic bacteria in cosmetics based on gene membrane chip technology

Resumen de: CN121718619A

The invention belongs to the technical field of cosmetic detection, and particularly relates to a method, a primer group and a kit for simultaneously detecting six pathogenic bacteria in cosmetics based on a gene membrane chip technology, co-enrichment culture, genome DNA extraction, multiple PCR amplification, gene membrane chip preparation and membrane chip color development detection, and an experimental result is judged according to the actual color development condition of a target. According to the present invention, the color developing points on the membrane chip are compared with the probe layout map, the internal reference color developing shows that the bacteria are detected, the color developing of each target point represents the detection of each specific bacteria, and the detection method has characteristics of simple operation, high accuracy, good repeatability, strong specificity, high stability and high sensitivity.

Integrated nano platform for bimodal detection and synergistic sterilization of pathogenic bacteria as well as preparation method and application of integrated nano platform

Resumen de: CN121720929A

The invention discloses an integrated nano platform for bimodal detection and synergistic sterilization of pathogenic bacteria as well as a preparation method and application of the integrated nano platform, and belongs to the technical field of food safety. The platform is composed of a multifunctional nano probe (MPDA/Pd SA-DA-CDs/Apt) and a magnetic separation assembly (MNRs/PGA/AM). The probe integrates enzyme-like catalysis and photo-thermal performance and is used for generating colorimetric and photo-thermal dual-mode signals so as to realize high-sensitivity and high-reliability detection of pathogenic bacteria. The magnetic separation assembly is used for specifically capturing and enriching target bacteria and eliminating matrix interference. After detection is positive, the platform can immediately start secondary propulsion synergistic sterilization: firstly, the action distance is shortened through magnetic aggregation, and then pathogenic bacteria are efficiently inactivated and biological membranes are disintegrated by utilizing the synergistic effect of near-infrared laser excitation photothermal effect and active oxygen. According to the invention, the integration of detection and disinfection is realized, the operation is convenient, and the method has important application value in the fields of food safety and biomedicine.

Anti-pollution microelectrode for identifying pseudomonas aeruginosa strain and detecting phenazine spatial distribution of pseudomonas aeruginosa strain as well as preparation method and application of anti-pollution microelectrode

Resumen de: CN121678796A

The invention belongs to the field of electrochemical sensors, and discloses an anti-pollution microelectrode for identifying pseudomonas aeruginosa strains and detecting phenazine spatial distribution of the pseudomonas aeruginosa strains as well as a preparation method and application of the anti-pollution microelectrode. The anti-pollution microelectrode comprises a carbon fiber electrode base body, a Fe-HHTP-PDA composite substrate layer and a Fe-HHTP-SBMA anti-pollution layer. The two-dimensional conductive metal organic framework material Fe-HHTP is doped in the anti-pollution layer based on the PDA substrate and the SBMA, so that the problem of signal attenuation caused by insulativity of the anti-pollution layer is successfully solved; by virtue of a two-dimensional ordered structure and a mixed valence iron node, the Fe-HHTP not only serves as a conductive bridge to maintain electron transmission, but also specifically catalyzes an oxidation-reduction reaction of phenazine substances, so that the anti-pollution function is realized, and meanwhile, the detection sensitivity is maintained and even improved. The preparation method is simple and convenient to operate and controllable in modification, and does not need a traditional high-pressure and high-temperature process.

Rapid drug sensitivity detection reagent and detection method for pathogenic bacteria of aquatic animals

Resumen de: CN121674527A

The invention relates to a rapid drug sensitivity detection reagent and detection method for pathogenic bacteria of aquatic animals, and the rapid drug sensitivity detection reagent for pathogenic bacteria of aquatic animals comprises the following components in percentage by weight: 1-3% of 2, 3, 5-triphenyl tetrazole chloride, 0.5-1% of iodonitrotetrazole, 5-10% of sodium chloride, 5-8% of sucrose, 0.1-0.5% of vitamin C powder and the balance of water. The invention also discloses a rapid drug sensitivity detection method of the detection reagent for pathogenic bacteria of aquatic animals. The rapid drug sensitivity detection reagent for the pathogenic bacteria of the aquatic animals is short in detection time, suitable for detection of various aquatic pathogenic bacteria, capable of supporting rapid screening of various antibiotics, low in cost and convenient to popularize and use in a large scale. The detection method is simple to operate and short in time consumption, can quickly complete drug sensitivity experiments of pathogenic bacteria of aquatic animals in batches, and is convenient for quickly and accurately screening out drugs for treating and preventing related bacterial diseases of the aquatic animals.

Salmonella lateral flow chromatography biosensor

Resumen de: CN121674407A

The invention provides a salmonella lateral flow chromatography biosensor. The invention particularly relates to a sandwich lateral flow chromatography sensor consisting of a nucleic acid nano enzyme and a bacteriostatic aptamer. When a target to be detected exists, the target to be detected is combined with the nucleic acid nano-enzyme probe on the combination pad, flows through the capillary action and is specifically intercepted by the Sal5-4-17nt aptamer when passing through the T line, and the redundant nucleic acid nano-enzyme probe continuously flows to the C line through the capillary action and is intercepted and captured through hybridization among nucleic acid sequences; therefore, after the color developing solution is added, obvious blue strips appear on T and C; when the to-be-detected target does not exist, the nucleic acid nano-enzyme probe is not intercepted by the T line and is intercepted only when the to-be-detected target passes through the C line, that is, only the C line has a blue band after the developing solution is added. The test paper can realize visual detection of salmonella within 5 minutes after probe dosage and hybridization sequence design and color developing solution and color developing time optimization, and meanwhile, the test paper has good biological safety.

Method for detecting residual blood through object surface disinfection wet tissue

Nº publicación: CN121678650A 17/03/2026

Solicitante:

BIWEI SHANGHAI MEDICAL DEVICES CO LTD
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Resumen de: CN121678650A

The invention provides a method for detecting residual blood through object surface disinfection wet tissue, and relates to the technical field of medical environment, medical equipment and equipment surface cleaning detection. After the object surface disinfection wet tissue with the residual blood detection function is used for collecting surface samples of a medical environment, a medical instrument and equipment, the residual blood pollution degree of the surfaces of the medical environment, the medical instrument and the equipment is qualitatively, semi-quantitatively and quantitatively judged according to the color change of the object surface disinfection wet tissue with the residual blood detection function. The semi-quantitative detection calibrator comprises multiple stages of color blocks and subdivision grids, and the residual blood volume is calculated by counting the area proportion of different color blocks through visual inspection; according to the quantitative detection AI rule, wet tissue images are scanned through photography and analysis equipment, software analyzes the mapping relation between pixel RGB values and standard concentration, the method is easy and convenient to operate, large-batch data can be rapidly processed, the cost is remarkably reduced, and the method is particularly suitable for rapid pollution evaluation of medical environments, medical instruments and equipment surfaces. Meanwhile, the pirameton can generate a composite reaction wi