Alerta

Detection method of strong-rotting-property pseudomonas reuneari

Resumen de: CN122146907A

The invention relates to a detection method of strong decay-causing pseudomonas reuni. The detection method comprises the following steps: extracting nucleic acid in a sample to be detected; carrying out recombinase-mediated amplification by taking nucleic acid as a template and adopting a specific RAA primer pair to obtain an amplification product; the RAA primer pair targets an aprX gene of the pseudomonas renenii, wherein the aprX gene contains a single nucleotide polymorphic site related to a strong rot-causing phenotype; finally, adding an amplification product into a CRISPR/Cas detection system, carrying out constant-temperature incubation, and then detecting a fluorescence signal in real time; the detection system comprises a Cas protein, a specific crRNA and a fluorescent report probe, and the crRNA can recognize a single nucleotide polymorphism site related to a strong rot-causing phenotype in a pseudomonas renenii aprX gene. According to the method, the detection accuracy is improved, the detection process is simple and convenient to operate, the reaction system is simple, the detection efficiency is improved, and the detection cost is reduced.

Fluorescent protein probe for noninvasive detection of myocardial cells and application of fluorescent protein probe

Resumen de: CN122145585A

The invention discloses a fluorescent protein probe for noninvasive detection of myocardial cells and application of the fluorescent protein probe, and belongs to the field of protein and gene engineering. Affinity peptides of myocardial cells are obtained by displaying a dodecapeptide library through bacteriophages, the affinity peptides are combined with EGFP and transferred into an expression vector, an escherichia coli expression system is used for expression, and a target fluorescent protein probe is obtained. Through experimental detection, the fluorescent protein probe can be used for detecting myocardial cells, real-time myocardial cell level detection is realized, and defects and barriers existing in traditional detection methods are broken through.

Method and system for detecting recombinant human interferon fermentation process parameters

Resumen de: CN122157794A

The invention discloses a method and a system for detecting fermentation process parameters of recombinant human interferon, belongs to the technical field of biological process monitoring and artificial intelligence, and is characterized in that prediction is carried out in the form of Raman spectrum data, so that damage to escherichia coli thalli is avoided, and the purpose of non-destructive prediction is achieved; moreover, training data is obtained by using a small-scale fermentation tank as an experimental data set and is applied to model training, so that a process parameter prediction model is quickly trained, nonlinear feature extraction and calculation which cannot be realized by a simple linear model are realized, accurate Raman spectrum features are obtained, the prediction accuracy is improved, and the prediction efficiency is improved. The method is verified through standard process parameter values of a large-scale fermentation tank, a final application model which can be directly applied to production is obtained, and real-time and accurate process parameter detection is carried out on actual production.

LFS-SERS rapid quantitative detection method for salmonella

Resumen de: CN122128407A

The invention discloses an LFS-SERS rapid quantitative detection method for salmonella, and belongs to the technical field of food safety, the method comprises the following steps: S1, cracking a sample to be detected to obtain a crude DNA solution; s2, carrying out salmonella specific LAMP (loop-mediated isothermal amplification) by taking the crude DNA solution as a template, so as to obtain a biotin labeled product; and S3, mixing dCas9 protein, sgRNA, the SERS-labeled gold nanoparticle-probe conjugate, the biotin labeled product and a loading buffer solution, incubating at room temperature for 1-5 minutes, carrying out a chromogenic reaction through a lateral flow test strip, and carrying out SERS detection on a T line in LFS by using a portable Raman spectrometer connected with an intelligent terminal to realize quantitative detection of salmonella in a to-be-detected sample. The method is strong in specificity, high in sensitivity and good in stability, the whole detection process can be completed within 40 minutes, the cost is low, and the operation is simple and convenient.

Multiple digital PCR detection reagent, detection device and detection method for food-borne pathogenic bacteria

Resumen de: CN122104968A

The invention relates to a multiple digital PCR detection reagent, a detection device and a detection method for food-borne pathogenic bacteria. The invention provides an ultra-multiple food-borne pathogenic bacterium digital PCR (Polymerase Chain Reaction) detection system based on a multicolor coding technology. The system breaks through the physical limitation of a fluorescence channel through an innovative probe coding strategy and a signal decoding algorithm, realizes simultaneous absolute quantitative detection of seven or more food-borne pathogenic bacteria in a single reaction tube, and ensures that the system still has high specificity, high accuracy, wide linear range and good repeatability in a complex matrix.

Kit and method for on-site detection of clostridium perfringens

Resumen de: CN122104859A

The invention relates to the technical field of microbiological detection, in particular to a kit and a method for detecting clostridium perfringens on site. The method comprises the following steps: putting a to-be-detected sample into a sample dilution tube for dilution and standing, dipping a sampling cotton swab into supernatant liquid, then vertically inserting the sampling cotton swab into a detection tube, retaining a cotton swab rod in the tube, and standing and recondensing to form a dual anaerobic sealing system; selecting room-temperature culture or constant-temperature culture at 37 DEG C for 2-6 hours according to the environment temperature; and observing the blackening condition of the culture medium to judge that blackening becomes positive and no blackening becomes negative. According to the method, professional equipment such as an anaerobic incubator and a sterile operation table is not needed, operation is simplified, complex pretreatment is not needed, the result can be rapidly judged and read within 2-6 h, full-process detection can be completed on sites such as farms and outdoors without laboratory conditions, and the problems that an existing detection method is high in equipment dependency degree, tedious in operation, long in period, insufficient in anaerobic guarantee and the like are effectively solved; the method is suitable for base-level and on-site rapid screening application.

SYSTEM AND METHOD FOR DETECTING, ENUMERATING, OR EXTRACTING MICROORGANISMS IN A SAMPLE

Resumen de: US20260146994A1

A system and method for detecting, enumerating, or extracting microorganisms in a sample is disclosed. Target microorganisms, such as Salmonella bacteria, may be of interest. Magnetic beads may be bound to the target microorganisms. After which, the bead-bound cells may be isolated. For example, a magnetic field may be applied in order to separate the target cells (with the magnetic beads attached thereto) and move then to a predetermined section of the well. Agar, or other immobilizing agent, may be added to the wells in order to immobilize the target cells. After which, the target cells are incubated and periodically analyzed to determine whether the target cells are growing, thereby indicating that the microorganisms are contained within the well.

MULTIPLE DETECTION COMBINATION FOR DETECTING VIRAL,

Resumen de: CN122095108A

Described herein are compositions, methods, and kits for detecting diarrhea-causing pathogens from a patient, food, or environmental sample. One embodiment described herein is a primer pair and probe for a multiplex polymerase chain reaction (PCR)-based assay for the detection of gastrointestinal pathogens, these gastrointestinal pathogens such as adenovirus, astrovirus, rotavirus, Norovirus, fiveleaf virus, Campylobacter strain, Clostridium difficile, Enteroaggregating Escherichia coli (EAEC), Enteropathogenic Escherichia coli (EPEC), Enterotoxigenic Escherichia coli (ETEC), Shiga-like toxin producing Escherichia coli (STEC), Shigella/Enteroinvasive Escherichia coli (EIEC), Escherichia coli O157, Escherichia coli O157, Escherichia coli O157, Escherichia coli O157, Escherichia coli O157, Escherichia coli O157, Escherichia The composition is prepared from the following raw materials: bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, listeria monocytogenes, plesiomonas shigelloides, salmonella strains, yersinia enterocolitica, vibrio strains, cryptosporidium strains, banquette sporidium, entamoeba histolytica, giardia intestinal or giardia blue. Other embodiments include methods and kits for detecting gastrointestinal pathogens.

Composite amplification system and detection kit for respiratory tract pathogenic bacteria and drug-resistant genes

Resumen de: CN122081528A

The invention belongs to the technical field of molecular detection, and particularly relates to a detection kit for respiratory tract pathogenic bacteria and drug-resistant genes based on a capillary electrophoresis detection platform and application of the detection kit. Compared with a traditional method, the method has the advantages that common respiratory tract infection pathogenic bacteria and drug-resistant genes are covered, detection sites are more comprehensive, specificity is higher, sensitivity is better, reliability is higher, and batch detection capacity is achieved.

Dual fluorescent quantitative PCR (Polymerase Chain Reaction) primer and probe for detecting isospora suis and enterotoxigenic escherichia coli and application of dual fluorescent quantitative PCR primer and probe

Resumen de: CN122081521A

The invention belongs to the technical field of molecular biological detection, and particularly relates to a dual fluorescent quantitative PCR primer and probe for detecting isospora suis and enterotoxigenic escherichia coli and application of the dual fluorescent quantitative PCR primer and probe. The invention discloses a dual fluorescent quantitative PCR (Polymerase Chain Reaction) primer and a probe. The dual fluorescent quantitative PCR primer comprises an upstream primer ITS1-F, a downstream primer ITS1-R, a probe ITS1-Probe, an upstream primer ST-F, a downstream primer ST-R and a probe ST-Probe, wherein the upstream primer ITS1-F is shown as SEQ ID NO.1, the downstream primer ITS1-R is shown as SEQ ID NO.2, the probe ITS1-Probe is shown as SEQ ID NO.3, the upstream primer ST-F is shown as SEQ ID NO.4, the downstream primer ST-R is shown as SEQ ID NO.5, and the probe ST-Probe is shown as SEQ ID NO.6, and the upstream primer ST-F is shown as SEQ ID NO.4, the downstream primer ST-R is shown as SEQ ID NO.6. The dual fluorescent quantitative PCR primer and probe provided by the invention have the advantages of high sensitivity, high specificity and good repeatability, can synchronously detect isospora suis and enterotoxigenic escherichia coli, and can improve the diagnosis efficiency.

Rapid screening and quantitative detection method for salmonella in grease feed additive

Resumen de: CN122084896A

The invention discloses a rapid screening and quantitative detection method for salmonella in a grease feed additive, and relates to the technical field of feed additive detection, the detection method comprises the following steps: matrix compatible pretreatment; selective affinity enrichment: a rapid screening channel and a quantitative channel; and the inner part participates in matrix response calibration. Through matrix compatible pretreatment of reversible lipid shielding, interface interference of grease on a capture layer and a detection reaction is reduced, and the problems of antibody ligand site shielding, reagent adsorption, nuclease reaction inhibition and the like caused by free grease in a high-grease matrix are solved; target mark damage or detection failure caused by pure strong chemical degreasing is avoided, the downstream capture and detection sensitivity is remarkably improved on the premise of not damaging target recognition sites, the sample compatibility is improved, the field pretreatment complexity is shortened, and rapid quality inspection process landing is facilitated.

Primer and probe combination for detecting avian salmonella based on RAA-LFD method and application of primer and probe combination

Resumen de: CN122081526A

The invention belongs to the technical field of molecular biological detection, and particularly relates to a primer and probe combination for detecting avian salmonella based on an RAA-LFD method and application of the primer and probe combination. The invention particularly relates to a primer and probe combination for detecting three avian serotype salmonella such as salmonella pullorum, salmonella enteritidis and salmonella typhimurium based on an RAA-LFD method and application of the primer and probe combination. Different types of probes and primers are respectively designed aiming at genes of serotype salmonella SP, SE and STM, rapid, sensitive and specific detection of salmonella and three serotype avian salmonella such as SP, SE and STM can be realized by adopting the primer and probe combination, the detection period of avian salmonellosis is greatly shortened, the detection time is shortened, and the detection efficiency is improved. The detection efficiency and accuracy of the avian salmonellosis are improved.

CRISPR-Cas12a/Cas12b-based composition and method for simultaneously detecting staphylococcus aureus and salmonella and application of CRISPR-Cas12a/Cas12b-based composition and method

Resumen de: CN122081529A

The invention discloses a composition for simultaneously detecting staphylococcus aureus and salmonella based on CRISPR-Cas12a/Cas12b, a method and application, and belongs to the technical field of food safety detection. Based on the characteristics of RPA isothermal amplification and preference of different Cas12 enzyme cleavage positions, a dual RPA-CRISPR-Cas12a/Cas12b detection method for staphylococcus aureus and salmonella is established through primer screening and optimization of parameters such as crRNA, reaction time and temperature, and the problem that it is difficult to detect multiple DNA targets of a Cas12 system at the same time is effectively solved. The method is good in specificity, high in sensitivity and simple in result interpretation, the detection limits of pure cultures and artificially polluted samples can reach the order of magnitude of 100 CFU/mL, detection can be completed within 1 h, and a new technical path is provided for rapid screening of food-borne pathogenic bacteria.

Reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) detection method aiming at escherichia coli total ribonucleic acid (RNA) residues and application

Resumen de: CN122081523A

The invention belongs to the technical field of biological detection, and particularly relates to a high-sensitivity and high-specificity quantitative detection method for total RNA (ribonucleic acid) of Escherichia coli remained in the production process of biological products (such as recombinant proteins, vaccines, cell therapy products and the like). Particularly relates to detection system construction, primer probe design and standardized operation processes based on a reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) technology, and further expands application of the detection system construction, primer probe design and standardized operation processes in drug quality control, process verification and cleaning verification.

Holding type listeria monocytogenes specific molecular target and rapid detection method thereof

Resumen de: CN122060880A

The invention discloses a persistent listeria monocytogenes specific molecular target and a rapid detection method thereof, and belongs to the technical field of microbiological detection. The molecular target is obtained through bioinformatics analysis and screening, is further screened through specific experimental verification, and is respectively specific genome sequence fragments of six retention types of listeria monocytogenes CC5, CC8, CC31, CC87, CC121 and CC204. A corresponding specific primer group is designed based on the target, sample DNA is amplified through PCR, a product is subjected to visual analysis in combination with gel electrophoresis, and whether the target retention type strain exists or not can be judged according to the size of a band. The detection method has the advantages of being short in detection time, low in cost, simple and convenient to operate and high in specificity, results are accurate, judgment is easy, practicability is high, the limitation that traditional culture and identification periods are long, and phenotypic analysis steps are tedious is broken through, and the detection method is suitable for rapid screening of the persistent listeria monocytogenes in samples of food, environment and the like.

Primer group, kit and detection method for detecting salmonella enteritidis

Resumen de: CN122060881A

The invention belongs to the technical field of microbiological detection and molecular diagnosis, and discloses a primer group, a kit and a detection method for detecting salmonella enteritidis, the kit comprises an immunocapture component, an isothermal amplification component, a CRISPR/Cas12a detection component and auxiliary components; the isothermal amplification component is any one of an RPA (recombinase polymerase amplification) component or an LAMP (loop-mediated isothermal amplification) component, the RPA component comprises a specific primer group targeting 86th to 212th nucleotides in a salmonella enteritidis hilA gene coding region, and the LAMP component comprises a specific primer group targeting 58th to 250th nucleotides in the salmonella enteritidis hilA gene coding region. The primer group, the kit and the detection method for detecting the salmonella enteritidis successfully integrate three key technologies of RAP and LAMP rapid isothermal amplification, CRISPR/Cas12a high-specificity recognition and trans-cleavage and immunomagnetic bead targeted enrichment, and creatively provide the primer group, the kit and the detection method for detecting the salmonella enteritidis.

Au-TiO2 coating-based pathogenic bacteria splitting decomposition and hydrogel in-situ detection method

Resumen de: CN122060843A

The invention discloses a pathogenic bacteria splitting decomposition and hydrogel in-situ detection method based on an Au-TiO2 coating, and belongs to the technical field of biology. The method comprises the following steps: (1) preparing an Au-TiO2 composite coating on a substrate; (2) dropwise adding a to-be-detected liquid sample to the surface of the Au-TiO2 composite coating, and covering the hydrogel sheet after the solvent is evaporated; or adhering the hydrogel sheet to the surface of the solid sample to be detected, stripping the adsorbed pathogenic bacteria, and covering the Au-TiO2 composite coating with the hydrogel sheet; the hydrogel sheet contains lysozyme, an isothermal amplification reaction reagent and a fluorescent dye; and (3) incubating the reaction system under an isothermal amplification reaction condition, and carrying out fluorescence imaging after the reaction is finished. According to the method provided by the invention, through hydrogel fixation and synergistic effect of lysozyme and Au-TiO2, splitting decomposition of pathogenic bacteria on an interface layer is promoted, in-situ nucleic acid amplification is realized, and the method has an extremely high application value for on-site rapid detection of pathogenic bacteria of agricultural products.

Kit and detection method for detecting multiple piglet diarrhea pathogens

Resumen de: CN122060931A

The invention provides a kit for detecting multiple piglet diarrhea pathogens and a detection method, and belongs to the technical field of biological information detection. The kit comprises a reaction reagent, wherein the reaction reagent comprises a porcine epidemic diarrhea virus specific primer and a probe, a porcine delta coronavirus specific primer and a probe, a piglet pathogenic escherichia coli specific primer and a probe, a clostridium perfringens specific primer and a probe, a buffer solution, an enzyme mixed solution and a freeze-drying protective additive. The detection method adopts the kit for detecting various piglet diarrhea pathogens. According to the kit disclosed by the invention, simultaneous detection of four target genes including the porcine epidemic diarrhea virus, the porcine delta coronavirus, the piglet pathogenic escherichia coli and the clostridium perfringens can be realized only through one-time amplification, so that the detection time is greatly shortened while the detection cost is reduced; the kit has extremely high specificity, sensitivity and repeatability, and the primers and the probes do not interfere with each other; good normal-temperature storage stability is also realized.

Rapid and accurate detection method for pathogenic bacteria

Nº publicación: CN122060834A 19/05/2026

Solicitante:

JIANGSU ACAD OF AGRICULTURAL SCIENCES
YANGZHOU YANGZHOU UNIV KANGYUAN DAIRY CO LTD
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Resumen de: CN122060834A

The invention discloses a rapid and accurate detection method for pathogenic bacteria. The method comprises the following steps: immobilizing bacteriophages on the surfaces of magnetic nanoparticles through physical adsorption or chemical reaction, selectively enriching pathogenic bacteria in a complex sample by adopting the bacteriophage functionalized magnetic nanoparticles, quickly cracking the pathogenic bacteria on the surfaces of the magnetic nanoparticles by using CTAB (cetyltrimethyl ammonium bromide), releasing ATP in the bacteria, and quantitatively analyzing ATP by using bioluminescence. Further calculating the content of the pathogenic bacteria; the detection method is free of enrichment and amplification, can complete capture, enrichment and detection of pathogenic bacteria within 20 minutes, and can specifically detect viable bacteria of target bacteria in various complex matrix (such as food) samples due to the fact that bacteriophages are adopted as recognition molecules.