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Beta-carotene hydroxylase mutant, preparation method and application

Resumen de: CN120866251A

The invention discloses a beta-carotene hydroxylase mutant as well as a preparation method and application thereof, and belongs to the technical field of microalgae gene engineering. Amino acid Cys No. 191 of haematococcus pluvialis beta-carotene hydroxylase is mutated into Ala, amino acid Thr No. 213 of haematococcus pluvialis beta-carotene hydroxylase is mutated into Ala, and the yield of astaxanthin is increased by 1.282 times and 1.268 times respectively. According to the mutant obtained through mutation, the catalytic efficiency and substrate selectivity of beta-carotene hydroxylase are remarkably improved, astaxanthin is produced more specifically through enzymatic reaction, and a key mutation direction is provided for construction of engineering strains for high yield of astaxanthin.

In-situ online observation device applied to microalgae growth dynamic response process

Resumen de: CN120870119A

The invention provides an in-situ online observation device applied to a microalgae growth dynamic response process. The in-situ online observation device comprises an in-situ online observation device and an automatic shooting system, the in-situ online observation device comprises a culture observation plate, a mainstream micro-channel and a micro-culture chamber; the culture observation plate is transparent; the mainstream micro-channel is arranged on the upper side of the culture observation plate; a flow inlet and a flow outlet are respectively formed in two ends of the main flow micro-channel in the length direction; the plurality of micro-culture chambers are communicated with the main flow micro-channel; the plurality of micro-culture chambers are arranged between the flow inlet and the flow outlet; the automatic shooting system is used for shooting microalgae in the micro-culture chamber; the in-situ online observation device is simple and effective in structure and convenient to manufacture, and has the characteristics that internal nutrients and metabolic oxygen are efficiently transported and transferred, and the nutrients are continuously, stably and uniformly distributed; and in combination with an automatic shooting system, long-time continuous imaging observation can be carried out on the intrinsic dynamic response process of the growth of a plurality of microalgae cells, and theoretical guidance is provided for the maximum growth potential of the microalgae c

Ecological bait production method and equipment based on food chain

Resumen de: CN120858911A

The invention discloses an ecological bait production method based on a food chain, which comprises the following steps: S1, mixing wine brewing enzyme liquid and culture tail water in proportion, and stirring to form a nutrient medium required by microalgae growth; s2, conveying the nutrient medium into a microalgae culture module, and regulating and controlling illumination and dissolved oxygen conditions; part of microalgae is conveyed to the plankton breeding unit to cultivate plankton to construct a live bait chain; s3, tail water generated by breeding flows back to the plankton breeding unit to serve as a nutrition supplement source of plankton, and closed-loop circulation is achieved.

Infrared light driven microalgae photosynthetic carbon sequestration method and application

Resumen de: CN120866183A

The invention discloses an infrared light driven microalgae photosynthetic carbon sequestration method and application, and belongs to the technical field of photosynthetic organism culture.The method comprises the steps that a near-infrared up-conversion nanometer material is dispersed in a microalgae culture medium, and a full-spectrum or near-infrared light source is used for promoting microalgae photosynthetic carbon sequestration; the near-infrared up-conversion nano material is a rare earth nano material with a NaYF4 inert shell structure. According to the method, the overall sunlight utilization efficiency of the microalgae is improved, and the photosynthetic carbon sequestration capacity of the microalgae is finally improved.

Microalgae culture method

Resumen de: CN120866071A

The invention relates to the technical field of microalgae culture, and discloses a microalgae culture method. The invention relates to a microalgae culture method, which comprises the following steps: (1) in the presence of a fat degradation inhibitor, culturing microalgae species to obtain a microalgae solution; (2) separating, adsorbing and degerming the algae solution to obtain a culture solution; and (3) returning the culture solution to the step (1) for reuse. The method can effectively control the generation of organic matters with a growth inhibition effect in the microalgae culture process, improve the production rate and reduce the culture water consumption.

APPARATUS FOR MICROALGAL OR CYANOBACTERIA GROWTH, METHODS AND USES THEREOF

Resumen de: WO2025224659A1

The present disclosure relates to an apparatus for microalgae and/or cyanobacteria cell culture comprising a unitary body formed from a hydrophobic material; and two or more microcapillary channels extending along the unitary body; wherein the inner surface of the microcapillary channels is coated with nutrients suitable for microalgae and/or cyanobacteria cell culture; and wherein the hydrophobic material is transparent to visible light, and to a kit and sensor thereof. A method for culturing microalgae and/or cyanobacteria in an apparatus comprising a unitary body formed from a hydrophobic material and two or more microcapillary channels extending along the unitary body, wherein the hydrophobic material is transparent to visible light is also disclosed.

LIPID PRODUCING MARINE MICROALGA

Resumen de: WO2024132882A1

The invention provides marine microalgal strain of the Ulvales order of the Ulvophyceae class which have utility in the efficient and environmentally beneficial preparation of triacylglycerides and polyunsaturated fatty acids, which can in turn be used for fuels or feeds.

METHOD FOR THE PREPARATION OF A CYANOBACTERIA EXTRACT COMPRISING PHYCOCYANIN ENRICHED IN TRACE ELEMENTS FROM NATURAL TRACE ELEMENT SOURCES FOR THE PRODUCTION OF FOOD SUPPLEMENTS

Resumen de: WO2024133379A1

The invention relates to a method for preparing a cyanobacterium extract, preferably spirulina, comprising phycocyanin enriched in trace elements. The invention also relates to an extract obtained by the method according to the invention, to a composition comprising said extract and to the use thereof.

Microalgae culture solution recycling method

Resumen de: CN120841600A

The invention relates to a microalgae culture solution recycling method, which comprises: carrying out solid-liquid separation on a microalgae culture solution to obtain a filtrate; enabling the filtrate to pass through a resin column and collecting effluent; the effluent is optionally mixed with a supplement and then returned for microalgae culture. By adopting the method for recycling the microalgae culture solution, wastewater discharge in the microalgae culture process can be effectively reduced, the wastewater biochemical treatment pressure is reduced, after the treated effluent is recycled for microalgae culture, the higher microalgae biomass content can still be obtained, the water consumption of microalgae production is remarkably reduced, and the method has a good application prospect.

Microalgae expanding culture method, microalgae expanding culture system and ecological restoration method

Resumen de: CN120843281A

The invention relates to the technical field of algae culture, in particular to a microalgae expanding culture method, a microalgae expanding culture system and an ecological restoration method. The microalgae expanding culture method comprises the following steps: inoculating a microalgae seed solution into a first culture solution, and carrying out first amplification treatment under the illumination condition of 100-500lux to prepare a first algae seed mixed solution; inoculating the first algae seed mixed solution into a second culture solution, and carrying out second amplification treatment under the illumination condition of 1000-3000 lux to prepare a second algae seed mixed solution; inoculating the second algae mixed solution into a third culture solution, and performing third amplification treatment at the pH value of 6.5-10 to prepare a third algae mixed solution; inoculating the third algae mixed solution into a fourth culture solution, and carrying out fourth amplification treatment under a stirring condition to prepare a fourth algae mixed solution; wherein the volumes of the first culture solution, the second culture solution, the third culture solution and the fourth culture solution are sequentially increased. According to the expanding culture method, the microalgae grow fast, and the culture period can be effectively shortened.

Crust biological agent as well as preparation method and application thereof

Resumen de: CN120843118A

The invention relates to the technical field of crust preparation preparation, in particular to a crust biological preparation, a preparation method and application thereof, and the preparation method comprises the following steps: S1, preparing a bacillus modifier; s2, preparation of a microalgae-biopolymer mixed solution; s3, primary compounding; and S4, compounding to form the agent. Through the synergistic effect of the bacillus modifier and the microalgae-biopolymer mixed solution, the biological crust forming period is remarkably shortened, the defect that natural recovery is slow (dozens of years) is overcome, meanwhile, the crust forms a compact net-shaped structure and strongly bonds soil particles, and the wind erosion rate is reduced by 50%. The freeze-drying process is improved, the biological activity is better reserved, the stability of the preparation is improved, the preparation is convenient to store and transport, the powder which is crushed and sieved (100 meshes) can be directly scattered, and field planting is further accelerated.

Method for purifying breeding wastewater

Resumen de: CN120841711A

The invention discloses a purification method of breeding wastewater, and relates to the field of wastewater treatment.The purification method comprises the following steps that straw is taken to be subjected to enzymolysis to prepare straw hydrolysate, and the straw hydrolysate, microalgae and spores of fungi are added into the breeding wastewater to be subjected to oscillation co-culture; the microalgae comprise chlorophyta algae, and the fungi comprise aspergillus fungi. According to the method, a phycomycetes purification system is formed in the sewage through the chlorophyta and the aspergillus fungi, the straw hydrolysate is matched to provide nutritional ingredients such as saccharides and amino acid for phycomycetes, the stability degree of the content of the nutritional ingredients in the sewage is improved, and under the comprehensive action, the removal rate of ammonia nitrogen and total phosphorus in the sewage is increased, and the ammonia nitrogen and the total phosphorus are converted into biomass.

Lipid-producing marine microalgae

Resumen de: CN120835926A

The present invention provides strains of marine microalgae of the ulva lactuca, which can be used for the efficient and environmentally beneficial preparation of triacylglycerides and polyunsaturated fatty acids, which in turn can be used in fuels or feeds.

Method for preparing microalgae protein bait by efficiently treating livestock and poultry biogas slurry by coupling aquatic vegetables with microalgae

Resumen de: CN120827149A

The invention belongs to the technical field of bioengineering and the field of sewage treatment, and particularly relates to a method for efficiently treating livestock and poultry biogas slurry to prepare microalgae protein bait by coupling aquatic vegetables with microalgae, which comprises the following steps: carrying out primary treatment on livestock and poultry wastewater by utilizing the aquatic vegetables, and carrying out secondary treatment on the microalgae with high robustness by coupling; the pollutant content of the biogas slurry is reduced, microalgae protein bait is harvested, and pollutants such as COD content, ammonia nitrogen content and phosphorus content in the livestock and poultry biogas slurry are efficiently reduced. According to the method, the pollutant content of the livestock and poultry biogas slurry is efficiently reduced, the COD content of the treated biogas slurry is reduced by 75%-90%, the ammonia nitrogen content is reduced by 80%-92%, the phosphorus removal rate reaches 90% or above, the biomass of microalgae harvested at low cost through self-flocculation reaches 2 g/L or above, the protein content in the microalgae reaches 50%-70%, the content of essential amino acids in the microalgae is close to that of fish meal, and the content of organic matters in the microalgae is reduced by 50%-70%. The feed can be used as high-quality protein bait.

Strain for degrading aspartame, algal-bacterial symbiotic system and application of algal-bacterial symbiotic system

Resumen de: CN120829860A

The invention discloses a bacterial strain for degrading aspartame, a bacterial-algae symbiotic system and application of the bacterial strain and the bacterial-algae symbiotic system, the bacterial strain is corn sphingomonas zeae NCU J1, and the preservation number of the bacterial strain is CCTCC (China Center for Type Culture Collection) NO: M 20251462. And an algal-bacterial symbiotic system is constructed by adopting an oriented symbiotic combination of the corn sphingomonas NCU J1 and the chlorella sp. The corn sphingomonas NCU J1 can metabolize an artificial sweetening agent aspartame and degradation products thereof, metabolism complementation can be achieved through an algal-bacterial symbiotic interface, and bacteria preferentially promote aspartame to be subjected to photohydrolysis to generate degradation products such as diketopiperazine derivatives. The microalgae synchronously start isomerism enzymatic reaction to convert degradation products such as diketopiperazine derivatives into low-toxicity products, the risk of secondary pollution is reduced by 40% or above compared with that of a traditional physical and chemical method, 150 mg/L aspartame can be efficiently degraded within 2 days, generation of derivatives of aspartame can be greatly reduced, secondary pollution is reduced, and the method is suitable for industrial production. And the method has a good application prospect in the aspect of rapidly treating aspartame-containing wastewater.

PROCESSES FOR PRODUCING BIOFERTILIZERS FROM BIOMASS AND PRODUCTS PRODUCED

Resumen de: WO2025221466A1

The invention relates to a method of producing a phototrophic cyanobacteria based biofertilizer.

METHOD FOR ENHANCING GROWTH OF DUNALIELLA SPECIES AND BIOMASS PRODUCTION THEREFROM

Resumen de: US2025327096A1

A method for enhancing growth of a microalgal species and its biomass production yield by co-cultivating the microalgal species with a bacterial isolate under the genus of Pseudoalteromonas in separate compartments. The microalgal species is selected from Dunaliella species. The genome sequence of the Pseudoalteromonas isolate can have a nucleotide similarity of lower than 95% to that of other Pseudoalteromonas species.

Method for Tissue Renewal Using A Microalgae-based Biologically Friendly Base Formula To Reverse Disease Progression And Foster Tissue Regeneration In Conditions Of Mucosal Injury And Inflammation In the Mouth And Gastrointestinal Tract

Resumen de: US2025325609A1

Provided is a composition comprising microalgae and clay, formulated for ingestion, application to skin, an oral cavity, or administration rectally to a mammal. Also provided is a method of treating a mammal. The method comprises administering the above composition to the mammal.

CONTINUOUS ALGAL AQUACULTURES

Resumen de: FI20255069A1

The present approach describes continuous, steady state algal aquaculture systems, methods, and uses. Algal aquaculture systems may employ a plurality of algal aquaculture stages, first to grow the microalga of interest, and subsequent stages for subjecting the microalga of interest to controlled stress. Algae biomass composition in the last algal aquaculture stage can be selectively determined through various process elements to impact product generation and/or to reduce the recalcitrance of the algal biomass towards downstream processing, such as fracking the cells to enable chemical-free harvesting via, e.g., adsorptive bubble technology.

CONTINUOUS ALGAE BIOMASS CULTIVATION IN OPEN SYSTEMS

Resumen de: FI20255068A1

The present approach describes continuous, steady state algal aquaculture systems, methods, and uses. Algal aquaculture systems may employ an algal aquaculture zone to grow the microalga of interest, and a collector for receiving a continuous stream of algal aquaculture medium for fracking and harvesting.

Microalgae production monitoring device

Resumen de: CN223458324U

The utility model discloses a microalgae production monitoring device which comprises a fixing assembly and a monitoring assembly, the monitoring assembly comprises an LED lamp set, a color sensor, a controller and an audible and visual alarm, the controller is electrically connected with the color sensor and the audible and visual alarm, the fixing assembly comprises a shading cylinder, and the shading cylinder is electrically connected with the LED lamp set. A pipeline mounting channel is arranged in the center of the interior of the shading cylinder, a monitoring opening is formed in one side of the center of the pipeline mounting channel, light transmitting openings are formed in the positions, located on the two sides of the monitoring opening, of the center of the pipeline mounting channel, and the detection end of a color sensor is mounted at the monitoring opening. The growth state of the microalgae can be accurately reflected, so that the real-time monitoring of the microalgae growth process is realized. Compared with a traditional naked eye observation method or an indirect parameter speculation method, the monitoring device can rapidly and accurately obtain microalgae growth state information, and the monitoring efficiency is greatly improved.

Feeding training method for wild euchiloglanis kishinouyei

Resumen de: CN120814499A

The invention relates to the technical field of wild euchiloglanis kishinouyei domestication methods, in particular to a wild euchiloglanis kishinouyei domestication method. According to the feeding training method for the wild euchiloglanis kishinouyei, cobblestones attached with microalgae are adopted to simulate the feeding environment of the wild euchiloglanis kishinouyei. According to the characteristic that wild euchiloglanis kishinouyei has a scraper type ingestion mode, microalgae are cultivated and attached to cobblestones, the cobblestones attached with the microalgae are utilized, the natural ingestion environment of euchiloglanis kishinouyei at the bottom of a river is simulated, the wild euchiloglanis kishinouyei can open firstly for ingestion in an artificial breeding environment, the breeding environment can be adapted as soon as possible, the stress reaction is reduced, and the breeding efficiency is improved. The feeding training success rate of the wild euchiloglanis kishinouyei is greatly increased, and the survival rate of the wild euchiloglanis kishinouyei

Phosgene synergetic microalgae bioreactor

Resumen de: CN120818425A

The invention belongs to the technical field of bioreactors, and particularly discloses a phosgene synergetic microalgae bioreactor which comprises a barrel body, the bottom of the barrel body is fixedly connected with a gas collection hopper, the lower part of the gas collection hopper is connected with a Venturi nano-bubble generator, the Venturi nano-bubble generator is connected with a liquid delivery pump, and the liquid delivery pump is connected with a liquid storage tank. A bubble remover, a light guide structure and a thermometer are detachably connected to the barrel body, the light guide structure comprises optical fibers inserted into the barrel body at equal intervals, each optical fiber comprises an outer sleeve inserted into the barrel body, and a light guide fiber core is fixedly connected to the interior of each outer sleeve. In the actual use process of the reactor, the Venturi nano bubble generator can convey bubbles into the barrel body, the bubbles can drive microalgae in the barrel body to float and can also refract light rays projected by the light guide fiber core, the light rays can uniformly cover the interior of the barrel body, and then the microalgae are efficiently cultured.

Telescopic claw type microalgae photobioreactor with built-in light arrangement

Resumen de: CN120796052A

The invention relates to the technical field of microalgae carbon sequestration, and discloses a telescopic claw type microalgae photobioreactor with built-in light arrangements, which comprises a reactor main body, a light adjusting assembly, a light adjusting part and a plurality of lamp tubes, a telescopic frame is arranged in the reactor main body, and the light adjusting part is arranged at the top end of the reactor main body. A center shaft of the telescopic frame is rotationally connected to the middle of the reactor top plate, the illumination adjusting part is arranged on the center shaft, the lamp tubes are arranged on the illumination adjusting part in the circumferential direction, and the distance and the illumination angle between the lamp tubes and the microalgae are adjusted through the illumination adjusting part; the gas adjusting part is arranged at the bottom of the reactor main body, and external gas is conveyed into the reactor main body through the gas adjusting part. The photosynthesis efficiency and carbon sequestration capacity of microalgae can be remarkably improved, and the device is compact in structure, energy-saving and environment-friendly, can be widely applied to the fields of industrial carbon capture, urban air purification, ecological restoration and the like, and provides important technical support for achieving the aim of carbon neutralization.

Compound enzyme-bacterium synergistic preparation for controlling cyanobacterial bloom as well as preparation method and use method of compound enzyme-bacterium synergistic preparation

Nº publicación: CN120796238A 17/10/2025

Solicitante:

SUZHOU TONGBIAO URBAN CONSTRUCTION OPERATION CO LTD
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Resumen de: CN120796238A

The invention relates to the technical field of cyanobacterial bloom control in water, and particularly discloses a compound enzyme-bacterium synergistic preparation for cyanobacterial bloom control, a preparation method and a use method. The preparation comprises the following components in percentage by mass: compound enzymes (10-20% of cellulase, 8-12% of beta-1, 3-glucanase and 3-8% of lipase), functional bacteria (10-20% of bacillus subtilis, 8-15% of trichoderma harzianum and 5-10% of bacillus amyloliquefaciens), an enzyme stabilizer (3-8% of trehalose), an adsorption carrier (15-25% of Fe < 3 + > modified charcoal and 8-15% of diatomite) and auxiliary components. The preparation method comprises the steps of carrier modification, solid fermentation and microcapsule embedding. During use, 3-20kg/1000m < 3 > of water is added for the first time, 2-10kg/1000m < 3 > of water is added every 7-14 days in the maintenance stage, and synchronous aeration is performed. Through the synergistic effect of beta-1, 3-glucanase and trichoderma harzianum, the algicidal rate is 92%, and the spore germination inhibition rate is 82%; the Fe < 3 + > modified charcoal improves the stability of the enzyme in an alkaline environment (80% of activity is retained when the pH value is 9); the method is suitable for eutrophic water with chlorophyll a greater than or equal to 30 mu g/L, and has no chemical residue risk.