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Method for improving soil fertility and enzyme activity of rice field by using nitrogen-fixing cyanobacteria biological membrane

Resumen de: CN119506138A

The invention relates to a method for improving soil fertility and enzyme activity of a rice field by using a nitrogen-fixing cyanobacteria biological membrane, which comprises the following steps: step 1, expanding propagation of nitrogen-fixing cyanobacteria: selecting anabaena globifera as a rice field application algae species, and performing expanding culture on the algae species to obtain a nitrogen-fixing cyanobacteria secondary algae species; step 2, applying nitrogen-fixing cyanobacteria: applying the nitrogen-fixing cyanobacteria in a rice base fertilizer period and a tillering period, and replacing part of chemical fertilizer with the nitrogen-fixing cyanobacteria to reduce the application amount of the chemical fertilizer; secondly, the nitrogen-fixing cyanobacteria returns to the rice field through biological nitrogen fixation and rotting decomposition of dead algae, the content of nitrogen and carbon in the soil is increased, the soil fertility is improved, and the nitrogen-fixing cyanobacteria can provide nutrient substances for microorganisms in the soil, improve the enzyme activity of the soil and improve the quality of the soil when applied to the rice field.

Closed marine microalgae culture system

Resumen de: CN119506048A

The invention relates to the field of microalgae culture, in particular to a closed marine microalgae culture system which comprises a greenhouse, a water pool is arranged in the greenhouse, a buoyancy device and a wave making device float in the water pool, a photosynthesis device is arranged on the buoyancy device and is in an upward convex shape, and the photosynthesis device comprises a transparent outer shell and a transparent inner shell. A photosynthesis cavity is formed between the transparent outer shell and the transparent inner shell, an exhaust valve is arranged at the top of the transparent outer shell and communicated with the photosynthesis cavity, the bottoms of the transparent outer shell and the transparent inner shell are both connected with the annular bottom shell in a sealed mode, and a feeding port and a discharging port are formed in the annular bottom shell. A gas uniformizing component is arranged at the bottom of the photosynthesis cavity. The greenhouse provided by the invention can provide a good environment for marine microalgae culture, and is beneficial to the growth of microalgae; meanwhile, the light receiving area of the photoreactor is large, and algae are not easily adsorbed on the container wall; the photoreactor floats in the water tank, so that stirring of a culture solution in the photoreactor is facilitated.

Method for regulating and controlling synthesis of microalgae fatty acid by using long-chain carbon signal substance and application of long-chain carbon signal substance

Resumen de: CN119506183A

The invention provides a method for regulating and controlling synthesis of microalgae fatty acid by using a long-chain carbon signal substance and application thereof, and the method for regulating and controlling synthesis of microalgae fatty acid by using the long-chain carbon signal substance comprises the following steps: culturing microalgae by using a culture medium to obtain a microalgae culture solution; then, a long-chain carbon signal substance is prepared into a solution, the long-chain carbon signal substance is C14-HSL, and the structural formula of the C14-HSL is shown in the formula I; adding a long-chain carbon signal substance solution into the microalgae culture solution to obtain a mixed solution; and culturing the mixed solution. According to the method, the C14-HSL signal substance is inoculated into the microalgae culture solution, so that the growth of microalgae can be effectively promoted, the relative content and composition of saturated fatty acid, monounsaturated fatty acid and polyunsaturated fatty acid in microalgae cells are influenced, the content of fatty acid related to biodiesel precursor synthesis is increased, and the yield and quality of biodiesel are improved. # imgabs0 #

CO2 trapping and recycling photoconductive enhanced osmunda culture'light tree 'bioreactor

Resumen de: CN119506047A

The invention discloses a photoconductive enhanced osmunda culture'light tree 'bioreactor for CO2 capture and resource utilization. The bioreactor comprises a transparent tank body made of a transparent material, and refractive fibers and hollow fibers are embedded in the transparent tank body. The refractive fiber is made of a high-light-conductivity material, can introduce and uniformly distribute an external light source into the whole bioreactor, and provides necessary illumination conditions for microalgae. The reactor can effectively capture carbon dioxide and utilize microalgae for resourceful culture. The reactor system provides a suitable microalgae growth environment with sufficient light and sufficient carbon sources for microalgae through gas-liquid separation, and promotes rapid growth of microalgae. The reactor can be applied to the fields of industrial waste gas treatment, greenhouse gas emission reduction, biological energy production, aquaculture wastewater and the like. A CO2 trapping technology is organically combined with a CO2 biosynthesis and resource utilization technology, so that efficient trapping and resource utilization of CO2 are realized.

Method for screening and cultivating yellow chlorella pyrenoidosa mutant strain capable of rapidly growing

Resumen de: CN119506094A

The invention relates to the field of microalgae breeding, in particular to a method for screening and cultivating yellow chlorella pyrenoidosa mutant strains capable of rapidly growing, which mainly comprises the following steps: marking wild type chlorella pyrenoidosa on a flat plate, selecting single algae colonies for activation culture, and culturing the single algae colonies to obtain the yellow chlorella pyrenoidosa mutant strains. The yellow chlorella pyrenoidosa capable of rapidly growing is screened and cultured, the yellow chlorella pyrenoidosa is cultured for 4-6 days under the heterotrophic condition of 25-30 DEG C, the color characterization is stable, the hereditary character of the yellow chlorella pyrenoidosa can be judged to be relatively stable, the growth rate is high, the chlorophyll content is remarkably lower than that of wild type chlorella pyrenoidosa, the biomass of the next day can reach 2.1 g/L under the condition that the optimal carbon source is 5g/L, and the yield is high. Compared with wild type chlorella pyrenoidosa, the invention has the advantages that the content of xanthophyll and biomass are higher than those of wild type chlorella pyrenoidosa, the amino acid composition is excellent, the EAA/TAA value is 44.74%, and the EAA/NEAA ratio is 0.81, so that the chlorella pyrenoidosa has the potential of replacing traditional edible resources, can be used as an optimal substitute of traditional food due to the excellent protein quality, and bro

High-efficiency self-luminous fiber'light tree 'bioreactor and microalgae culture technology thereof

Resumen de: CN119506046A

A high-efficiency self-luminous fiber'light tree 'bioreactor is characterized in that components of the bioreactor are provided with light guide fiber tubes, membrane filaments of a hollow fiber membrane are prepared by blending polymers and self-luminous materials, and the hollow fiber membrane has high light transmission to promote photosynthetic biological reaction. The reactor can effectively capture carbon dioxide and utilize microalgae for resourceful culture. The reactor system provides a suitable microalgae growth environment with sufficient light and sufficient carbon sources for microalgae through gas-liquid separation, and promotes rapid growth of microalgae. The reactor can be applied to the fields of industrial waste gas treatment, greenhouse gas emission reduction, biological energy production, aquaculture wastewater and the like. A CO2 trapping technology is organically combined with a CO2 biosynthesis and resource utilization technology, so that efficient trapping and resource utilization of CO2 are realized.

Landscape lighting and outdoor algae ecological'light tree 'bioreactor

Resumen de: CN119506045A

The invention discloses a landscape illumination and outdoor algae ecological'light tree 'integrated bioreactor, which can provide illumination for algae by utilizing sunlight in the daytime, and can provide necessary illumination conditions for microalgae by utilizing artificial light sources such as street lamps and automobile light at night, and meanwhile, energy is saved. Through efficient photosynthesis, the reactor can remarkably absorb CO2 in the atmosphere, and greenhouse gas emission is reduced. The bioreactor further comprises a landscape lighting device, soft green light can be emitted at night by means of street lamps, the attractive landscape effect is formed, and the night visual attraction of a city is improved. The CO2 supply device ensures that the algae grow in a proper environment through CO2 absorbers arranged around the main body structure. The overall design not only has an environmental protection function, but also can be used as a part of urban landscape, enhances the aesthetic property and environmental awareness of cities, and is suitable for various outdoor environments such as urban green lands, parks and streets.

Method for determining exfoliation of outer membrane of cyanobacteria, device for determining exfoliation of outer membrane of cyanobacteria, and program

Resumen de: CN119522288A

The present disclosure provides a method for determining exfoliation of an outer membrane of cyanobacteria capable of easily determining whether or not the outer membrane has been exfoliated from a cell wall. A method for determining exfoliation of an outer membrane of cyanobacteria according to the present invention comprises: a measurement step (S01) for measuring the concentration of at least one amino acid selected from the group consisting of isoleucine, leucine, tyrosine and phenylalanine in a culture supernatant of cyanobacteria; and a determination step (S02) for determining whether or not the outer membrane of the cyanobacteria has been peeled off from the cell wall on the basis of the concentration of the at least one type of amino acid measured in the measurement step.

Method for controlling bacterial pollution in microalgae culture and culture device thereof

Resumen de: CN119506068A

The invention discloses a control method for bacterial pollution in microalgae culture and a culture device thereof, and relates to the field of microalgae culture, and the control method comprises the following steps: step 1, carbon dioxide supply: when microalgae are cultured in a transparent incubator, when a carbon dioxide sensor detects that carbon dioxide in the incubator is smaller than a preset interval, the carbon dioxide sensor detects that carbon dioxide in the incubator is smaller than the preset interval; the valve is opened, so that the carbon dioxide enters the air supply pipeline through the communicating pipe, the circular reducer pipe and the connecting pipe, and then is sprayed into the nutrient solution from the spray head; 2, carbon dioxide is dissolved, when the carbon dioxide passes through a round reducer pipe, a driving assembly in a treatment mechanism is driven, namely, an impeller rotates, the impeller transmits rotating force to a third straight gear, the rotating third straight gear drives a gear ring in a stirring assembly to rotate, and the carbon dioxide is stirred; a gear ring drives a stirring rod to stir a nutrient solution by controlling a horizontal plate to cooperate with sprayed bubbles, so that the dissolution of carbon dioxide is accelerated, the growth conditions of microalgae are optimized, and the growth of bacteria is inhibited; and step 3, cleaning the inner wall of the box: when a horizontal plate moves, pushing a pushing block

Microalgae culture method

Resumen de: CN119490944A

The invention relates to a microalgae culture method which comprises the following steps: culturing microalgae species in the presence of a fat-soluble additive to obtain a culture solution containing floating layer microalgae; and harvesting the microalgae on the floating layer. The fat-soluble additive is introduced in the microalgae culture process, the concentration of a microalgae floating layer can be effectively improved, the viscosity of the microalgae is improved, and therefore the culture efficiency and the harvesting efficiency of the microalgae are remarkably improved.

BIOACTIVE COMPOUNDS WITH PESTICIDE ACTIVITIES DERIVED FROM AGED CULTURES OF GREEN MICROALGAE

Resumen de: WO2025038756A1

Embodiments of the present disclosure pertain to a composition suitable for use in treating an agricultural material. In some embodiments, the composition includes a microalgae culture, an extract of the microalgae culture, compounds from the microalgae culture, or combinations thereof. Additional embodiments of the present disclosure pertain to methods of treating an agricultural material by applying a composition of the present disclosure to the agricultural material.

FERMENTED TEXTURED MICROALGAE

Resumen de: WO2023198694A1

The present invention relates to a method for producing fermented textured microalgae, comprising steps of mixing and incubating the microalgae with lactic ferments, and also the composition obtained using the method, and the use of the composition for food.

PROCESS FOR OBTAINING A TEXTURISING PROTEIN EXTRACT FROM MICROALGAE

Resumen de: WO2023198689A1

The present invention relates to a process for obtaining a protein extract from microalgae. The protein extract obtained has a texturising ability to form a protein network, pure or in a mixture with another protein source, allowing the formation of food gels, extruded protein material or cooked cereal products. The protein extract obtained also has properties of coagulation, pure or in a mixture with another protein source, allowing the formation of food products that require protein coagulation without the formation of a protein network, such as hot emulsions or desserts ("custards"). The invention also relates to the use of the protein extract for replacing the texturising functionalities provided by animal proteins (egg proteins, dairy proteins, red or white meat proteins) within these products, and therefore for replacing sources of animal protein within these products.

MODIFIED MICROALGAE FOR ENHANCED PHOSPHATE UPTADE INVOLVING OVEREXPRESSION OF PSR1 AND OPTIONALLY UNDEREXPRESSION OF PTC1

Resumen de: WO2023198774A1

The invention provides a recombinant microalgal strain comprising in its genome a first modification which causes overexpression of a PSR1 gene, and optionally a further modification which reduces or eliminates expression from an endogenous PTC1 gene. The strains of the invention have utility in promoting phosphate uptake, for example from wastewater, with the microalgae then being useful as fertilisers.

USE OF CYANOBACTERIAL BIOREACTOR FOR CARBON SEQUESTRATION

Resumen de: WO2023201190A1

The subject invention provides compositions and methods for reducing deleterious atmospheric gases using microorganisms. In preferred embodiments, a composition comprising one or more beneficial microorganisms convert carbon dioxide to calcium carbonate, magnesium carbonate, a bicarbonate, or a combination thereof. The source of the carbon dioxide can be industrial emissions. In some embodiments, the composition sequesters carbon with the use of a trough growth system and the culture and/or growth by-product can be sequestered in underground geological formations.

Preparation method of microalgae nano-selenium and application of biotransformed nano-selenium in prevention and treatment of plant late blight

Resumen de: CN119464400A

The invention discloses a preparation method of microalgae nano-selenium and application of biotransformed nano-selenium in prevention and treatment of plant late blight. The novel preparation method of the microalgae nano-selenium comprises the following steps: firstly, extracting an intracellular extract of chlorella pyrenoidosa, and then adding sodium selenite into the intracellular extract to carry out nano-selenium conversion; compared with an existing reported method that after sodium selenite is added into a microalgae living body culture solution to be converted into nano-selenium, algae is taken to break cells, and the nano-selenium is collected, the method has the advantages that the microalgae living body does not need to be used when the nano-selenium is biologically converted, and the subsequent complicated nano-selenium separation and purification steps can be avoided; the nano-selenium obtained by converting the intracellular extract can be directly applied. Experiments prove that the effect of the nano-selenium obtained through biotransformation on inhibiting phytophthora infestans of potato late blight pathogenic bacteria is obviously better than that of chemically synthesized nano-selenium, and the nano-selenium obtained through biotransformation has an obvious effect on preventing and treating potato late blight and has an obvious effect on promoting plant growth.

Pipeline type photobioreactor for microalgae culture

Resumen de: CN222499138U

The pipeline type photobioreactor comprises a bottom plate and a top plate, a plurality of cleaning assemblies are arranged between the bottom plate and the top plate, a plurality of reaction straight pipes I and reaction straight pipes II are arranged on the front side and the rear side of each cleaning assembly respectively, the reaction straight pipes II are located on the rear sides of the reaction straight pipes I, and the reaction straight pipes II are located on the rear sides of the reaction straight pipes II. According to the pipeline type photobioreactor, the inner side of the spiral main body of the pipeline type photobioreactor can be fully cleaned by utilizing the cleaning assembly, and a cleaning worker does not need to extend a flushing pipe into a gap of a spiral pipeline when cleaning the inner side of the spiral reactor main body; and a user does not need to worry that the flushing pipe collides with the reaction straight pipe I, the reaction straight pipe II and the reaction curved pipe during cleaning, so that the pipeline type photobioreactor is more convenient and simpler to clean.

Slow-flow thin-layer photobioreactor

Resumen de: CN222499090U

The utility model relates to the technical field of microalgae photobioreactors, and discloses a slow flow thin layer type photobioreactor which comprises a supporting frame and a water tank container installed on the supporting frame, the water tank container is arranged in an inclined mode, and a plurality of baffles which are arranged in a staggered mode are arranged in the water tank container in an array mode in the extending direction. Through cooperation of the baffles which are arranged in the water tank container and are arranged in a staggered mode, the flowing track of algae liquid is in an S shape, the flowing path of the algae liquid is greatly increased, and the retention time of the algae liquid in the water tank container is prolonged; the flow velocity required for maintaining the algae liquid with the same thickness is obviously smaller than that required when the baffle plates are not arranged, so that the energy consumption caused by overhigh flow velocity can be reduced, and the energy consumption can be greatly reduced by adjusting the distance between the two adjacent baffle plates and the width of the water tank reactor.

Bio-oxygen therapy and anti-oxidation dual-strategy soluble microneedle as well as preparation method and application thereof

Resumen de: CN119454566A

The invention relates to a biological oxygen therapy and antioxidation dual-strategy soluble microneedle as well as a preparation method and application thereof, and belongs to the technical field of medicines. The microneedle is an integrated soluble microneedle and comprises a matrix formed by a water-soluble polymer and a compound which is uniformly dispersed in the matrix and formed by microalgae and metal organic framework nano-enzyme, the compound formed by the microalgae and the metal organic framework nano-enzyme is a compound of a'core-shell 'structure, the metal organic framework nano-enzyme serving as the shell is wrapped on the surface of the microalgae serving as the core. The microneedle disclosed by the invention has the capability of rapidly resisting oxidation in situ and continuously and deeply conveying oxygen, is widely applied to preparation of medicine design or preparations for treating tumors, promoting wound healing, regulating an inflammation microenvironment, treating skin burns and scalds and removing scars, and is good in patient applicability; and the preparation process is green, environment-friendly, simple, rapid, mild in condition and suitable for large-scale production.

Extraction method of microalgae

Resumen de: CN119462252A

The invention discloses an extraction method of microalgae, and belongs to the technical field of microorganisms, and the extraction method comprises the three steps of microalgae culture, enzymolysis reaction and compounding aftertreatment. When a microalgae extract is applied to a microalgae fertilizer, the yield and quality of crops can be effectively improved; and the schizochytrium limacinum culture medium is treated by using p-hydroxybenzoic acid and diammonium phosphate, so that the cell membrane permeability of the schizochytrium limacinum is changed, other nutrient substances can enter the schizochytrium limacinum conveniently, and meanwhile, cell wall breaking of the schizochytrium limacinum is facilitated. Under a proper ratio of an enzymolysis mixture, pyrolytic biochar, bentonite and biomass tar, the prepared microalgae-biochar simultaneously has abundant pore structures, a large amount of microalgae loads and strong combination structural performance, the activity performance of the microalgae-biochar can be effectively maintained, and the application range of the microalgae-biochar is widened. The method has a wide market prospect when being used for preparing a microalgae fertilizer.

Method for relieving microalgae high-temperature stress and improving sewage treatment performance by utilizing ferroptosis inhibitor

Resumen de: CN119461667A

The invention discloses a method for relieving microalgae high-temperature stress and improving sewage treatment performance by utilizing a ferroptosis inhibitor. According to the method, a ferroptosis inhibitor Fer-1 is added into a microalgae growth environment, so that Fer-1 fully enters microalgae cells, and then the ferptosis inhibitor Fer-1 is applied to sewage treatment. According to the method, the ferroptosis inhibitor Fer-1 is added to regulate and control the way of programmed cell death of the microalgae, so that the inhibition effect of high temperature on the growth of the microalgae is remarkably relieved, the balance of an intracellular oxidation-anti-oxidation system is effectively maintained, the influence of a lipid metabolism way of the chlorella is relieved, high-temperature stress resistance is realized, and the yield of the chlorella is improved. Meanwhile, the nitrogen and phosphorus removal performance of the sewage is improved, the removal rates of ammonia nitrogen and phosphorus are improved by 44.50% and 30.72% respectively, and the method has important application value for effectively protecting microalgae cells under the extreme temperature condition and promoting low-carbon resourceful treatment of the microalgae in the sewage.

Device and method for evaluating developmental toxicity of high-concentration cyanobacteria toxin based on fish embryos

Resumen de: CN119464003A

The invention relates to a fish embryo-based high-concentration cyanobacteria toxin development toxicity evaluation device and method, and relates to the technical field of pollutant toxicity analysis, the device structure comprises a base and an electric guide rail mechanism 1; the carrier is arranged on the first electric guide rail mechanism in a sliding mode, a movable oscillation base is arranged on the carrier, a plurality of perforated plates used for placing fish embryos are arranged on the movable oscillation base, and a lighting structure is arranged at the position, located below the movable oscillation base, of the carrier; the liquid injection unit is used for injecting cyanobacteria toxin working solutions with different high concentrations into the porous plates, and an observation unit is arranged at the top of the incubation chamber; and the adjusting shading unit is arranged between the lighting structure and the perforated plate. The evaluation device provided by the invention is convenient to use, reduces the labor intensity of workers, improves the working efficiency, and facilitates large-scale and high-efficiency evaluation and analysis of the fish embryonic development toxicity of the high-concentration cyanobacteria toxin.

Ecological fertilization planting method and system for increasing selenium content in rice

Resumen de: CN119452855A

The invention discloses an ecological fertilization planting method and system for increasing the selenium content in rice, and relates to the field of ecological fertilization planting, and the ecological fertilization planting method comprises the following steps: establishing a microalgae culture pond, and culturing a specific microalgae variety capable of enriching selenium by using a selenium-rich water supply system; analyzing plant electric signal characteristics of rice corresponding to different selenium contents in each growth stage, and establishing a plant selenium absorption electric signal reaction mode library; optimizing and adjusting the application amount of microalgae liquid supplied to the paddy field by utilizing an intelligent irrigation system based on a matching result of a reaction mode library of a rice plant electric signal and a plant selenium absorption electric signal detected in real time; the residual selenium-rich microalgae liquid flows back to the culture pond to be recycled, and the selenium supplementing amount of the microalgae culture pond is dynamically regulated and controlled.

3D Bioink for 3D printing for microalgae water treatment carrier manufacturing

Resumen de: KR20250022553A

본 발명은 수처리 담체를 3D 프린팅하기 위한 3D 프린팅용 바이오잉크에 관한 것으로, 3D 프린터의 니들에서 출력되어 수처리 담체를 이루는 알지네이트 하이드로겔; 및 상기 알지네이트 하이드로겔에 혼합되어, 수처리 과정에서 폐수에 포함된 질소(N)와 인(P)을 제거하는 미세조류가 포함되어 이루어져, 미생물 고정화 기술에 3D 바이오 프린팅 기술을 적용하여 기존의 미생물 고정화 기술의 문제점을 극복할 수 있는 기능적이고 구조 안정적인 미세조류 고정화 담체를 제조할 수 있는 미세조류 수처리 담체 제조를 위한 3D 프린팅용 바이오잉크를 제공한다.

Preparation method of algal-bacterial symbiotic filler and filler composite structure based on algal-bacterial symbiotic filler

Nº publicación: CN119430494A 14/02/2025

Solicitante:

HOHAI UNIV
\u6CB3\u6D77\u5927\u5B66

Resumen de: CN119430494A

The invention discloses a preparation method of a bacteria-algae symbiotic filler, which specifically comprises the following steps: adding an activated sludge dispersion liquid into a reactor filled with a filler, operating for 7-10 days under an aeration condition, and introducing simulated tail water in the operation process; then chlorella is inoculated according to the volume ratio of the activated sludge dispersion liquid to the chlorella dispersion liquid being 1: (5-50), operation is conducted for 14-20 days at the temperature of 22-30 DEG C under illumination, and the light-dark ratio is that illumination is conducted for 6-12 h, and darkness is conducted for 12-18 h; tail water of the simulated sewage plant is introduced in the operation process; the filler hung with the algal-bacterial symbiotic biological membrane is obtained. In the formed algal-bacterial symbiotic membrane, the microalgae can promote enrichment of nitrogen and phosphorus removal flora and organic matter metabolism functional flora, and bacteria can improve the photosynthesis performance and carbon sequestration performance of the microalgae through material exchange, information transfer and other ways, so that the algal-bacterial symbiotic system can efficiently purify N/P/organic matter in a water body; meanwhile, the emission of greenhouse gas is reduced. The invention also discloses a filler composite structure based on the algal-bacterial symbiotic filler.