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CONTINUOUS MICROALGAE HARVESTING AND CULTIVATING APPARATUS

NºPublicación:  KR20250038982A 20/03/2025
Solicitante: 
한명호이신형정태성
KR_20250038982_PA

Resumen de: KR20250038982A

미세조류 배양 및 수확장치가 개시된다. 본 발명에 따른 미세조류 배양 및 수확장치는, 내부공간을 형성하는 프레임과, 투명재질로 이루어져 상기 내부공간의 전방을 개폐하도록 상기 프레임에 마련되는 도어와, 투명재질로 이루어지고 상기 프레임의 좌,우측과 후방에 결합되어 상기 내부공간을 외부와 구분하는 각각의 수직판재와, 배양액이 유입되기 위한 배관 연결구를 구비하여 상기 프레임의 상부에 결합되는 빛이 투과되도록 투명재질로 된 상부판재를 포함하는 본체; 메쉬가 서로 다른 메쉬망을 각각 구비하여 상기 본체의 내부공간에 수직방향으로 간격을 유지하여 각각 설치되되, 상부에서 하부로 갈수록 메쉬가 점차 작은 메쉬망이 위치하도록 설치되고, 상기 도어를 통하여 인출 가능하게 설치되는 다수개의 수확용 트레이; 및 상기 배관 연결구를 통하여 공급되는 배양액이 각 상기 수확용 트레이의 각 메쉬망을 통과한 후 수집되도록 상기 본체의 하부에 마련되는 임시 저장탱크를 포함하고, 상기 배관 연결구를 통하여 공급되는 배양액이 각 상기 수확용 트레이의 각 상기 메쉬망을 차례로 통과하여 상기 임시 저장탱크로 수집되는 과정에서 각 상기 메쉬망에 걸러진 미세조류가 공급되는 배양액에 의해 배양되고,

MICROALGAE CULTURING APPARATUS

NºPublicación:  WO2025058231A1 20/03/2025
Solicitante: 
FORNATURES CO LTD [KR]
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WO_2025058231_A1

Resumen de: WO2025058231A1

The present invention relates to a microalgae culturing apparatus comprising: a housing which has a predetermined installation space thereinside; a culture unit which is made of a transparent material and is provided in the form of a water tank having an algae culture space filled with microalgae and a culture medium therein so as to be capable of installation in or removal from the installation space in the housing; a culture unit connection part which is provided on one side of the installation space in the housing so as to, when the culture unit is placed and installed thereon, maintain a watertight state and have a connection structure in which the inside of the culture unit and the inside of the culture unit connection part communicate with each other; a lighting part which is installed on one side of the installation space in the housing and outputs and provides light toward the culture unit when the lighting unit is driven; an air supply unit which is connected to the lower portion of the culture unit connection part and provides an air supply path for supplying air introduced into the installation space from the outside of the housing to the algae culture space in the culture unit; and a culture medium circulation unit which is connected to the lower portion of the culture unit connection part so as to suck in the microalgae and the culture medium discharged to the lower portion of the algae culture space in the culture unit and circulate and discharge the microalgae

Photobioreactor

NºPublicación:  CN119639549A 18/03/2025
Solicitante: 
GUONENG BAORIXILE ENERGY LTD COMPANY
CHN ENERGY NEW ENERGY TECH RESEARCH INSTITUTE CO LTD
\u56FD\u80FD\u5B9D\u65E5\u5E0C\u52D2\u80FD\u6E90\u6709\u9650\u516C\u53F8,
\u56FD\u5BB6\u80FD\u6E90\u96C6\u56E2\u65B0\u80FD\u6E90\u6280\u672F\u7814\u7A76\u9662\u6709\u9650\u516C\u53F8
CN_119639549_PA

Resumen de: CN119639549A

The invention provides a photobioreactor. The photobioreactor comprises a light-permeable container; the first light supplementing assembly is installed in the light-transmitting container and located in the middle of the light-transmitting container; the reaction tubes are used for containing microalgae suspension liquid, and the multiple reaction tubes surround the first light supplementing assembly and are installed on the light-permeable container; the plurality of second light supplementing assemblies are mounted on one side, back on to the first light supplementing assembly, of the reaction tube; and a controller; wherein the controller can receive the illumination intensity information of the surface of the reaction tube and send instructions to the first light supplementing assembly and the second light supplementing assembly to adjust the light supplementing intensity, so that the stability of the illumination environment in the reactor is maintained. When the external illumination condition changes, the controller controls the light supplementing assembly to adjust the light supplementing intensity according to the monitored illumination intensity change so as to maintain the stability of the whole illumination environment in the reactor.

Device and method for improving transparency of natural water body by utilizing microalgae biological membrane

NºPublicación:  CN119638078A 18/03/2025
Solicitante: 
SHANGHAI INVESTIGATION DESIGN & RES INST CO LTD
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CN_119638078_PA

Resumen de: CN119638078A

The invention provides a device and a method for improving transparency of a natural water body by utilizing a microalgae biological membrane, and belongs to the field of water treatment, and the device comprises a water tank, rollers, a microalgae attachment matrix, a water distribution device and a lighting device. The transparency of the natural water body is effectively improved through the microalgae biological membrane. The microalgae biological membrane secretes macromolecular extracellular polymeric substances, can adsorb suspended particulate matters and soluble organic matters in water, and inhibits the reproduction of other planktons through nutrition competition, so that the turbidity is reduced. In addition, the device is designed as up-flow water inlet, so that the contact between the water body and the microalgae biological membrane is enhanced, and the treatment effect is improved. LED lamps and a heating device are adopted to keep a proper growth environment, and meanwhile, a photovoltaic power generation system is arranged, so that green and environment-friendly effects are achieved. The method is simple and easy to implement, low in cost and convenient to manage and maintain, and a novel and effective technical scheme is provided for improving the transparency of the natural water body.

Domestication method of microalgae and application of domesticated microalgae in treatment of sulfur-containing gas

NºPublicación:  CN119614473A 14/03/2025
Solicitante: 
CHINA PETROLEUM & CHEM CORP
SINOPEC NANJING CHEMICAL RES INST CO LTD
SINOPEC NANJING CHEMICAL IND CO LTD
\u4E2D\u56FD\u77F3\u6CB9\u5316\u5DE5\u80A1\u4EFD\u6709\u9650\u516C\u53F8,
\u4E2D\u77F3\u5316\u5357\u4EAC\u5316\u5DE5\u7814\u7A76\u9662\u6709\u9650\u516C\u53F8,
\u4E2D\u56FD\u77F3\u5316\u96C6\u56E2\u5357\u4EAC\u5316\u5B66\u5DE5\u4E1A\u6709\u9650\u516C\u53F8
CN_119614473_A

Resumen de: CN119614473A

The invention relates to the field of microalgae culture, in particular to a microalgae domestication method and application of domesticated microalgae produced by the method in treatment of sulfur-containing gas. The domestication method of the microalgae comprises the following steps: inoculating the microalgae to a domestication culture medium, introducing sulfur-containing gas, and sequentially carrying out at least two stages of domestication culture to obtain the domesticated microalgae. By adopting the domestication method of the microalgae, provided by the invention, the sulfur resistance and the desulfurization efficiency can be remarkably improved.

System for efficiently treating livestock and poultry breeding sewage by utilizing algal-bacterial symbiosis

NºPublicación:  CN119612846A 14/03/2025
Solicitante: 
DONGGUAN SHENGLONG ENVIRONMENTAL PROTECTION TECH CO LTD
\u4E1C\u839E\u5E02\u76DB\u9686\u73AF\u4FDD\u79D1\u6280\u6709\u9650\u516C\u53F8
CN_119612846_PA

Resumen de: CN119612846A

The invention relates to the technical field of sewage treatment, in particular to a system for efficiently treating livestock and poultry breeding sewage by utilizing algal-bacterial symbiosis, which comprises a solid-liquid separation module arranged on the ground, the solid-liquid separation module comprises a water inlet pipe, a separation box and a filter basket, a nitrification and denitrification tank is arranged on the top of the ground, and a stirring assembly is arranged in the nitrification and denitrification tank; the chemical phosphorus removal module comprises a coagulation, flocculation and sedimentation tank and a stirring assembly, the coagulation, flocculation and sedimentation tank is arranged on the ground, and the stirring assembly is arranged in a coagulation and flocculation area in the coagulation, flocculation and sedimentation tank; according to the system for efficiently treating the livestock and poultry breeding sewage by utilizing the bacterial-algae symbiosis, compared with an existing method for removing the total nitrogen content in the sewage by independently utilizing microorganisms, pollutants in the sewage are synergistically removed in the oxidation pond by utilizing a bacterial-algae symbiosis structure, microalgae and bacteria, so that the energy consumption and the carbon source are saved, and the environmental pollution is reduced. Meanwhile, the sewage treatment effect is improved.

Mudflat shellfish breeding method

NºPublicación:  CN119605706A 14/03/2025
Solicitante: 
SDIC BIOTECH INVEST CO LTD
\u56FD\u6295\u751F\u7269\u79D1\u6280\u6295\u8D44\u6709\u9650\u516C\u53F8
CN_119605706_PA

Resumen de: CN119605706A

The invention relates to the field of aquaculture, and discloses a mud flat shellfish breeding method which comprises the following steps: mixing algae powder and fresh microalgae in a feeding device to obtain bait; the baits are fed to mud flat shellfish larvae through a feeding device, and seedling raising is conducted; wherein in the bait, the cell number ratio of the algae powder to the fresh microalgae is 100 or less; wherein the mixing conditions are as follows: the pressure is negative pressure, and the time is more than 5 minutes. According to the method, the hidden danger that culture is unstable when the algae powder is used for replacing fresh algae can be reduced, and mixed feeding of the microalgae and the algae powder for full-artificial breeding of the mud flat shellfish is achieved.

Method for treating chlorella circulating culture solution through Fenton oxidative coupling activated carbon adsorption and application of chlorella circulating culture solution

NºPublicación:  CN119614375A 14/03/2025
Solicitante: 
UNIV CHINA PETROLEUM EAST CHINA
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CN_119614375_PA

Resumen de: CN119614375A

The invention belongs to the technical field of biological energy and environmental engineering, and particularly discloses a method for treating a chlorella circulating culture solution through Fenton oxidative coupling activated carbon adsorption to solve the growth inhibition phenomenon caused by extracellular polymeric substance accumulation in the microalgae circulating culture process so as to achieve cyclic utilization of the chlorella culture solution and application of the chlorella culture solution. By means of Fenton oxidative coupling activated carbon adsorption, chlorella extracellular polymeric substances (EPS) can be effectively degraded and oxidized and decomposed into small-molecule organic substances, the growth inhibition effect of the small-molecule organic substances on chlorella is relieved, an organic carbon source can be provided for growth and metabolism of chlorella, and growth of chlorella is remarkably promoted. According to the method, the chlorella circulating wastewater is treated and recycled through Fenton oxidative coupling activated carbon adsorption, so that the yield of chlorella biomass can be effectively increased, and the production and culture cost is reduced.

Photoelectric microfluidic automatic control method for screening microalgae growth conditions

NºPublicación:  CN119619027A 14/03/2025
Solicitante: 
UNIV HAINAN
\u6D77\u5357\u5927\u5B66
CN_119619027_PA

Resumen de: CN119619027A

The invention discloses a photoelectric microfluidic automatic control method for screening microalgae growth conditions, which is characterized by comprising the following steps: preparing different types of culture medium solutions, respectively introducing the culture medium solutions into a photoelectric wetting chip, and generating different types of culture medium drops on the photoelectric wetting chip; automatically obtaining the current positions and IDs of a plurality of culture medium drops, and automatically obtaining the current positions and IDs of algae drops; according to the current positions and the IDs of the plurality of culture medium drops and the current positions and the IDs of the algae drops, respectively planning moving paths of the plurality of culture medium drops to the corresponding algae drops; respectively driving the plurality of culture medium drops to move to the corresponding algae drops in parallel according to the moving path through the plurality of light spots for fusion; and culturing the fused liquid drops in an illumination incubator so as to screen out a culture medium solution which is most suitable for the growth of the algae drops.

Outdoor air clearing device using microalgae

NºPublicación:  KR20250036368A 14/03/2025
Solicitante: 
주식회사에스아이솔루션

Resumen de: KR20250036368A

본 발명은 내부에 유동공간이 마련되며, 상기 유동공간으로 공기가 유입될 수 있도록 유입구와 배출구가 형성된 본체와 상기 본체에 설치되어 공기를 상기 유입구로 유입되어 상기 배출구로 배출되도록 강제 송풍시키는 송풍유닛과 상기 유동공간 내에 설치되며, 내부에 미세조류가 생장되는 배양액이 수용되는 저수공간이 마련된 배양수조와, 상기 유동공간으로 유입된 공기 중 이산화탄소를 소비하여 산소가 발생되도록 상기 미세조류가 광합성할 수 있게 상기 배양수조 내에 광을 조사하는 광원부가 마련된 배양유닛 및 상기 유동공간으로 유입된 공기 중 이물질을 걸러낼 수 있도록 상기 배양수조에 수용된 배양액 중 일부를 상기 유동공간을 통과하는 공기와 접촉시킨 다음, 상기 배양수조로 회귀시키는 접촉유닛을 특징으로 한다. 본 발명에 따르면, 건물 밖에 야외에 설치되어 미세조류를 이용하여 공기 내의 산소 농도를 증가시키거나 먼지를 제거 할 수 있도록 하는 미세조류를 이용한 야외 공기 클리어 장치를 제공하는 것이다.

Preparation method and application of microalgae exosome-fucoxanthin composite nanoparticles

NºPublicación:  CN119606919A 14/03/2025
Solicitante: 
UNIV NINGBO
\u5B81\u6CE2\u5927\u5B66
CN_119606919_PA

Resumen de: CN119606919A

The invention discloses a preparation method and application of microalgae exosome-fucoxanthin composite nanoparticles, and the preparation method is characterized by comprising the following steps: adding a fucoxanthin ethanol solution and a microalgae exosome solution into a cuvette according to a mass ratio of (0.5-1.5): 1, co-incubating for 30 minutes, adding an electrotransfection buffer solution which is 1-3 times of the volume of the mixed solution, and uniformly stirring; the mixed solution in the cuvette is subjected to electroporation by using an electroporation instrument, electric shock is carried out by controlling the voltage to be 100-500 V and the capacitance to be 300-700 mu F, then incubation is carried out for 0.5-2 h at the temperature of 35-40 DEG C, the mixed solution is centrifuged for 20-40 min under the conditions of 80000-120000 g and the temperature of 4 DEG C, precipitation particles are collected, and the microalgae exosome-fucoxanthin composite nanoparticles are obtained. The application of the composite nanoparticle in preparation of an antioxidant, an ABTS free radical scavenger and/or a DPPH free radical scavenger has the advantages that the stability and bioavailability of fucoxanthin are improved, and a synergistic antioxidant effect is generated.

Microalgae culture medium and microalgae preservation method

NºPublicación:  CN119614374A 14/03/2025
Solicitante: 
UNIV NINGBO
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CN_119614374_PA

Resumen de: CN119614374A

The invention provides a microalgae culture medium and a microalgae preservation method, relates to the technical field of algae culture, and particularly provides a brand-new microalgae preservation liquid culture medium and a microalgae preservation solid culture medium, and develops a brand-new microalgae biphasic preservation method by using the microalgae preservation liquid culture medium and the microalgae preservation solid culture medium. The preservation method comprises the following steps: inoculating microalgae species in an exponential growth phase into a microalgae preservation solid culture medium, culturing for 5-10 days, and adding a microalgae preservation liquid culture medium into the microalgae preservation solid culture medium inoculated with the microalgae species to form a semi-solid culture medium, the semi-solid culture medium is subjected to biphasic preservation under natural light at 2-10 DEG C. The preservation method can effectively prolong the preservation time of microalgae species, ensures the preservation effect, and has wide application prospects.

Method for culturing beneficial algae by resource utilization of cyanobacterial bloom

NºPublicación:  CN119614376A 14/03/2025
Solicitante: 
FISHERY MACHINERY & INSTRUMENT RES INST CAFS
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CN_119614376_PA

Resumen de: CN119614376A

The invention provides a method for culturing beneficial algae by resource utilization of cyanobacterial blooms, which comprises the following steps: 1, obtaining cyanobacterial (microcystis) blooms, and concentrating the cyanobacterial (microcystis) blooms until the concentration of chlorophyll a is 80-150mg/L; 2, carrying out continuous hydrodynamic disturbance on the high-concentration cyanobacterial bloom, wherein the disturbance intensity is suitable for preventing the cyanobacterial bloom from blackening and stinking; 3, continuously disturbing for 20-60 days to obtain cyanobacterial bloom which turns light yellow green or white; 4, tap water is used for diluting cyanobacterial blooms with changing colors, the concentration during dilution is 0.5-2.3 mL/cm < 2 >, and beneficial algae such as diatom and green algae are inoculated for culture under the conditions of normal temperature and weak light; and 5, when the culture is performed for 12-30 days, the growth density of the beneficial algae reaches the highest peak, and the cell density of the beneficial algae can reach up to 2.5 * 10 < 6 >-5.6 * 10 < 7 > cells/cm < 2 >. According to the method, algal toxin can be removed, beneficial algae such as diatom and green algae can be cultured through resource utilization of the cyanobacterial bloom, and a new way is provided for control and resource utilization of the cyanobacterial bloom.

SUSTAINABLE CURTAIN WALL

NºPublicación:  US2025084355A1 13/03/2025
Solicitante: 
THE UNIV OF NORTH CAROLINA AT CHARLOTTE [US]
The University Of North Carolina At Charlotte
WO_2022081803_A1

Resumen de: US2025084355A1

A microalgae curtain wall includes photobioreactors, an interior glass panel, an exterior glass panel, and transoms. The photobioreactors are adapted to receive sunlight and carbon dioxide to grow microalgae received therein. The exterior glass panel is offset from the interior glass panel forming a gap therebetween. The transoms hold the interior glass panel and the exterior glass panel therebetween. The transoms suspend the photobioreactors in the gap and between the interior glass panel and the exterior glass panel.

MICROALGAE-BASED SOIL INOCULATING SYSTEM AND METHODS OF USE

NºPublicación:  US2025081908A1 13/03/2025
Solicitante: 
MYLAND COMPANY INC [US]
MYLAND COMPANY, INC

Resumen de: US2025081908A1

Some embodiments include a microalgae culturing system including a bioreactor adapted to propagate microalgae in a culture solution using in combination at least one of natural and artificial light, and at least one nutrient including at least a carbon source, where the microalgae are freely suspended in and form part of the culture solution. A microalgae feed source is coupled to the bioreactor and a first controller between a water conditioning assembly and the bioreactor. The water conditioning assembly is coupled as an input of supply water to the bioreactor, and configured to condition the supply water to a specified purity that enables substantially unhindered growth of the microalgae in the culture solution to a specified concentration, and the first controller is configured to control supply of the microalgae feed source to the bioreactor.

Method for fixing frequent peak regulation gas power plant flue gas carbon dioxide through chlorella

NºPublicación:  CN119592425A 11/03/2025
Solicitante: 
UNIV CHONGQING
STATE POWER INVEST ZHUHAI HENGQIN THERMOELECTRICITY CO LTD
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\u56FD\u7535\u6295\uFF08\u73E0\u6D77\u6A2A\u7434\uFF09\u70ED\u7535\u6709\u9650\u516C\u53F8
CN_119592425_PA

Resumen de: CN119592425A

The invention relates to a gas power plant flue gas treatment technology, and aims to provide a method for fixing frequent peak regulation gas power plant flue gas carbon dioxide through chlorella. The method comprises the steps that high-temperature flue gas of a gas power plant is cooled and filtered, obtained reaction gas is introduced into a chlorella carbon sequestration system, the chlorella carbon sequestration system is composed of a high-carbon grease algae powder area, a medium-carbon protein algae powder area and a low-carbon starch algae powder area, and each area comprises a photobioreactor containing chlorella liquid and a culture medium; sequentially connecting the photobioreactors of the three subareas so that the photobioreactors operate in a series connection manner; the change of the growth stage of chlorella is influenced by changing different nitrogen element content conditions and illumination conditions, so that the growth carbon sequestration metabolic pathway of chlorella is regulated and controlled in the face of CO2 with different concentrations. According to the invention, the growth habit adjustment of chlorella can be controlled by using different nitrogen environments and illumination conditions, so that the microalgae carbon sequestration system can adapt to the CO2 concentration change in a wider range.

Genetically engineered algae for inhibiting protozoa as well as construction method and application of genetically engineered algae

NºPublicación:  CN119592426A 11/03/2025
Solicitante: 
UNIV NINGBO
NINGBO YUJIAN HEALTH TECH CO LTD
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\u5B81\u6CE2\u7389\u5065\u5065\u5EB7\u79D1\u6280\u80A1\u4EFD\u6709\u9650\u516C\u53F8
CN_119592426_PA

Resumen de: CN119592426A

The invention discloses a genetically engineered algae for inhibiting protozoa and a construction method and application thereof, and is characterized in that the genetically engineered algae is phaeodactylum tricornutum into which an expression plasmid pPha-T1-eGFP-Linker-EP2 is introduced, and the nucleotide sequence of the expression plasmid is as shown in SEQ ID NO: 7. The construction method comprises the following steps: artificially synthesizing an EP2 coding sequence optimized by a codon; a step of inserting the expression vector into the downstream of the eGFP of the expression vector, and expressing a plasmid pPha-T1-eGFP-Linker-EP2; the method comprises the following steps of: electrically transforming expression plasmids into phaeodactylum tricornutum, and coating transformed phaeodactylum tricornutum cells on an f/2 plate culture medium for culturing and screening positive algae strains to obtain the genetically engineered algae for inhibiting protozoa, and has the advantages that the large-scale culture efficiency and success rate of microalgae are improved, and the genetically engineered algae have a strong inhibition effect on Tophelenium sectorius and Tophelenium lakeri.

Transgenic synechocystis algal strain with enhanced stress resistance and application of transgenic synechocystis algal strain in sewage treatment

NºPublicación:  CN119592601A 11/03/2025
Solicitante: 
UNIV NORTHWEST A&F
\u897F\u5317\u519C\u6797\u79D1\u6280\u5927\u5B66
CN_119592601_PA

Resumen de: CN119592601A

The invention discloses a transgenic synechocystis strain with enhanced stress resistance and application of the transgenic synechocystis strain in sewage treatment, and belongs to the technical field of microorganisms. According to the invention, the MntH gene is integrated into the genome of the synechocystis PCC6803, so that the transgenic synechocystis PCC6803 is constructed. Through stress of Cr < 6 + > metal ions and hydrogen peroxide, the difference of stress resistance between algae strains before and after transformation is verified. The result shows that the stress resistance of the synechocystis strain is effectively improved by inserting the functional element gene MntH into the genome of the synechocystis PCC6803. Under stress culture of hydrogen peroxide or Cr < 6 + > metal ions, the transgenic algal strain shows better stress resistance, the growth condition is better than that of a wild algal strain, and meanwhile, the content of photosynthetic pigment, protein and the like related to the growth of the transgenic algal strain is higher than that of the wild algal strain. The invention provides a technical means for constructing transgenic algal strains with enhanced stress resistance and researching physiological and metabolic functions of microalgae.

Organic wastewater treatment device coupling photocatalysis and microalgae carbon sequestration

NºPublicación:  CN119591286A 11/03/2025
Solicitante: 
UNIV CHONGQING JIAOTONG
\u91CD\u5E86\u4EA4\u901A\u5927\u5B66
CN_119591286_PA

Resumen de: CN119591286A

The invention discloses an organic wastewater treatment device coupling photocatalysis and microalgae carbon sequestration. The organic wastewater treatment device comprises a photocatalytic degradation device, a microalgae carbon sequestration device and a light guide nuclear pore filter membrane, the front end of the photocatalytic degradation device is provided with a water inlet, and the tail end is provided with a water outlet of which the pipe diameter is smaller than that of the water inlet; the light guide nuclear pore filter membrane is mounted at the top of the photocatalytic degradation device above the baffle plate; a feeding hole is formed in the top of the microalgae carbon sequestration device; a detachable visible light emitting plate is arranged in the microalgae carbon sequestration device. According to the method, electricity is generated in the photocatalytic degradation process, waste recycling is achieved, and generated carbon dioxide provides a carbon source for carbon sequestration microalgae. The microalgae absorb carbon dioxide generated by photocatalysis through photosynthesis. The microalgae cells are converted into energy substances such as biodiesel while realizing emission reduction, so that the effects of saving energy, reducing emission and improving the water treatment efficiency are realized.

Screening method of floating microalgae suitable for floating culture and floating culture method of microalgae

NºPublicación:  CN119592438A 11/03/2025
Solicitante: 
CHINA PETROLEUM & CHEM CORP
SINOPEC RES INSTITUTE OF PETROLEUM PROCESSING
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CN_119592438_PA

Resumen de: CN119592438A

The invention relates to the field of microalgae, and discloses a floating microalgae screening method suitable for floating culture and a microalgae floating culture method.The screening method comprises the steps that microalgae is inoculated and then cultured for 24 h or above, then standing is conducted for 6-12 h, the proportion of the dry weight of algae cells floating within 1 cm of the liquid level to the total dry weight of algae is measured, and the total dry weight of the algae is calculated according to the ratio of the dry weight of the algae cells floating within 1 cm of the liquid level to the total dry weight of the algae; screening microalgae with the proportion of more than 25wt% to obtain floating microalgae; wherein the culture time enables the OD (optical density) of the algae in the algae liquid to reach more than 0.2; wherein the standing condition is as follows: the illumination intensity is 20000 to 40000 lux. The screened spirulina can be used for floating culture, so that the biomass equivalent to that under a stirring culture condition can be obtained while the energy consumption caused by driving the spirulina liquid is greatly reduced.

Chlorella pyrenoidosa mutant strain and application thereof

NºPublicación:  CN119570629A 07/03/2025
Solicitante: 
SHENZHEN HAISENDE TECH CO LTD
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CN_119570629_PA

Resumen de: CN119570629A

The invention relates to the technical field of microalgae biology, in particular to a chlorella pyrenoidosa mutant strain and application thereof. According to the invention, a mutagenesis screening mode is adopted to obtain a chlorella pyrenoidosa mutant strain which is named as RMCP012, the chlorella pyrenoidosa mutant strain can still grow quickly after being continuously cultured for one year and is stable in character, and the protein content of the chlorella pyrenoidosa mutant strain is equivalent to that of a wild type chlorella pyrenoidosa mutant strain. Meanwhile, the algal strain of the mutant strain is light green, the decoloring process of industrial production of algal source protein is greatly reduced, the industrial cost is reduced, and the mutant strain is a high-quality algal strain capable of being used for industrial production of protein. The invention further relates to a heterotrophic culture mode of the chlorella pyrenoidosa, so that the chlorella pyrenoidosa can grow quickly, and protein with a relatively high level can be accumulated.

Combined carbon sequestration method for treating radioactive strontium pollution of seawater by porphyridium

NºPublicación:  CN119569240A 07/03/2025
Solicitante: 
UNIV XIAMEN
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CN_119569240_PA

Resumen de: CN119569240A

The invention relates to the technical field of bioengineering, in particular to a combined carbon sequestration method for treating radioactive strontium pollution of seawater by porphyridium. The method comprises the following steps: inoculating a porphyridium strain into a radioactive strontium polluted seawater culture medium for treatment, adding a certain content of inorganic salt and trace element solution, continuously illuminating, and controlling the temperature, illumination intensity, sterile mixed gas ventilation rate and carbon dioxide ventilation concentration to realize the effect of treating seawater radioactive strontium pollution by porphyridium and combining carbon sequestration. The concentration of strontium element in seawater and the fixation of carbon dioxide are realized by utilizing the growth of porphyridium. According to the method, microalgae carbon sequestration is combined with wastewater treatment and biofuel production, so that the method has remarkable environmental and economic advantages, and the production cost and the energy consumption can be reduced to a certain extent. The method is easy to operate and low in cost and has certain universality.

Blue-green algae separation and purification method

NºPublicación:  CN119570683A 07/03/2025
Solicitante: 
SHENZHEN ACADEMY OF ENV SCIENCES
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CN_119570683_PA

Resumen de: CN119570683A

The invention provides a cyanobacteria separation and purification method. The method comprises the following steps: collecting a water sample; diluting the water sample; a step of culturing; a selection step; selecting a culture hole only with a single filament and without other cells by using a microscope, selecting a single algal filament from the remaining culture holes under the microscope by using a glass capillary tube, removing impurities and foreign matters attached to the surfaces of the algal cells, and transferring the algal cells into another culture hole for continuous culture; and enlarging the culture step. According to the method, the dilution culture method and the capillary separation method are combined for separation and purification of the cylindrospora, so that the separation and purification efficiency is improved.

Microalgae saline-alkali soil conditioner and application thereof

NºPublicación:  CN119570630A 07/03/2025
Solicitante: 
XINJIANG JINZHENG BIOTECHNOLOGY CO LTD
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CN_119570630_PA

Resumen de: CN119570630A

The invention discloses a microalgae saline-alkali soil conditioner and application thereof, and belongs to the technical field of microalgae saline-alkali soil conditioners. The microalgae saline-alkali soil conditioner disclosed by the invention is prepared from three microalgae which are respectively chlorella sorojin, nodularia harneri and chlamydomonas reinhardtii, and the volume percentages of the three microalgae are respectively 40 to 60 percent, 20 to 30 percent and 20 to 30 percent. The invention further provides a method for improving saline-alkali soil by using the microalgae saline-alkali soil conditioner, after the microalgae saline-alkali soil conditioner is used, the saline-alkali content of the soil can be effectively reduced, the salt content of a saline-alkali soil plough layer is reduced by 10%-30%, and the pH value is reduced by 0.2-0.8. The product and the method provided by the invention have good application prospects in the aspect of saline-alkali soil improvement, and are worthy of vigorous popularization.

Solid microalgae-containing composition for culturing fresh microalgae as well as preparation method and application of solid microalgae-containing composition

Nº publicación: CN119570778A 07/03/2025

Solicitante:

INST PROCESS ENG CAS
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CN_119570778_PA

Resumen de: CN119570778A

The invention relates to the field of microalgae culture, in particular to a solid microalgae-containing composition for culturing fresh microalgae as well as a preparation method and application of the solid microalgae-containing composition. The composition containing the solid microalgae and used for culturing the fresh microalgae comprises live microalgae cells and food-grade gel. The viable microalgae cells are algae species with an appropriate pH value of 6 to 8.2 and/or algae species with an appropriate pH value of 8.3 to 13. The food grade gel is insoluble in the culture medium of microalgae and can support the growth of live microalgae cells. The composition containing the solid microalgae is aseptically packaged in the soft photobioreactor, and can be preserved for a long time. By utilizing the composition containing the solid microalgae disclosed by the invention, the sanitary fresh microalgae which can be directly eaten can be easily prepared by residents. According to the method, the sanitation problem of preparing the fresh microalgae by an existing liquid culture process is solved, and the tedious processes of harvesting algae cells, washing algae mud, cleaning and maintaining a culture device and the like in liquid culture can be avoided.

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