Alerta

- Recombinant Microbials Expressing Strep-tag and Tumor Imaging Aduvant Comprising the Microbials

Resumen de: KR20240012662A

The present invention relates to a genetic construct characterized in that the anti-sigma factor flgM gene and the gene encoding strep-tag are linked to encode a fusion protein of flgM and strep-tag so that a strep-tag may be expressed on the surface of an anticancer strain that may be targeted to a tumor site and stimulate immune activity, and an anticancer adjuvant and a tumor imaging aid which is transformed thereby and exhibits anticancer activity itself and may be usefully used for the diagnosis and treatment of tumor cells together with an anticancer substance or contrast agent labeled with a substance which specifically reacts with the strep-tag.

一种用于防治多种致病性肠杆菌感染的融合蛋白及应用

Resumen de: CN120842432A

本发明涉及一种应对多种致病性肠杆菌感染防治的融合蛋白、免疫原性组合物、重组简并疫苗，以及分子架构设计和应用等。本发明筛选到3种细胞免疫抗原Tuf、DnaK、fusA，构建的3种抗原的融合蛋白分子可显著抑制多种肠杆菌感染引起的组织病变，具有良好的免疫原性，起到有效防治和免疫保护作用，广谱且高效地预防多种致病性肠杆菌感染，具有广阔的工业应用前景。

免疫原性组合物

Resumen de: WO2024192346A1

Technologies for providing immunogenic compositions (e.g., vaccines) and methods of inducing immune responses in subjects in need thereof.

- Recombinase polymerase amplification primer sets for detecting Salmonella and uses thereof

Resumen de: KR20250151120A

본 발명은 살모넬라(Salmonella) 검출을 위한 재조합효소-중합효소 증폭법(recombinase polymerase amplification)용 프라이머 세트 및 이를 이용한 핵산 측면 흐름 면역 분석(nucleic acid lateral flow immunoassay)용 살모넬라(Salmonella) 검출용 키트에 관한 것으로, 본 발명의 프라이머 세트는 살모넬라(Salmonella)속에 특이적인 유전자인 invA 유전자를 가장 높은 효율로 증폭하여 살모넬라(Salmonella) 검출이 우수할 뿐만 아니라, 재조합효소-중합효소 증폭(recombinase polymerase amplification) 수행시 비교적 낮은 온도에서 짧은 시간안에 증폭이 용의할 뿐만 아니라 상기 프라이머 세트의 역방향 프라이머 말단에 제1 표지물질이 결합하여 검출 가능한 발색입자인 금나노입자가 결합되고, 상기 프라이머 세트가 증폭하는 서열의 일부 서열에 특이적으로 결합하는 부위를 포함하는 프로브는 5' 말단에 제2 표지물질이 결합하여 핵산 측면 흐름 면역 분석(nucleic acid lateral flow immunoassay)에 적용시 RPA 증폭산물을 쉽게 검출 할 수 있는 살모넬라(Salmonella) 검출용 프라이머 세트 및 이의 용도를 제공한다.

STRUCTURED MICROGEL ELECTROPHORETIC ARRAYS FOR RAPID MULTIPLEX NUCLEIC ACID DETECTION

Resumen de: US2025321205A1

Methods and devices are disclosed for rapid, multiplex molecular detection of diverse nucleic acid target molecules. The invention features an electrophoretic array with immobilized hydrogel microgel deposits. Each deposit comprises a three-dimensional, cross-linked polymer matrix containing an immobilized affinity-binding molecule and a porogen-derived pore network. This structure is configured for rapid molecular transport of nucleic acids (e.g., up to 800 bp), providing a localized environment for target capture, ligation of linear Rolling Circle Amplification (RCA) probes, and RCA. Target-specific components are anchored within distinct microgels for multiplexing. Electric fields enhance transport, reaction kinetics, and amplicon concentration. Detection is achieved in under 20 minutes. The specifically structured and fabricated microgels improve detection speed, sensitivity, and applicability to multiple different targets.

DUAL-MODE ELECTROCHEMICAL POINT-OF-CARE DETECTION, QUANTIFICATION AND PROFILING OF PATHOGENS

Resumen de: US2025321227A1

The present invention relates to diagnostic platform. More specifically, the invention relates to a dual platform composed of a detection and quantification unit and a characterization unit, systems, kits, methods and uses thereof in detection, quantification and characterization of pathogens, the system comprising monoclonal-antibody-based biosensor chips, for detection and/or quantification of pathogens in a sample, and substrate-based biosensor chips for enzymatically profiling the pathogen in the sample.

BIOMARKER COMPOSITION FOR PREDICTING HYPOPIGMENTATION KIT USING THE SAME AND INFORMATION PROVISION METHOD USING THE SAME

Resumen de: KR20250148070A

본 발명은 저색소침착증 예측용 바이오마커 조성물, 이를 이용한 키트 및 이를 이용한 정보제공방법에 관한 것으로, 보다 구체적으로는 코리네박테리움 세그토섬(Corynebacterium segmentosum), 코리네박테리움 투베르쿨로스테아리쿰(Corynebacterium tuberculostearicum), 큐티박테리움 아크네스(Cutibacterium acnes), 큐티박테리움 그래눌로섬(Cutibacterium granulosum), 로소넬라 클리블란덴시스(Lawsonella clevelandensis), Propionibacterium sp. oral taxon 193, 슈도모나스 올레오보란스(Pseudomonas oleovorans), 슈도모나스 푸티다(Pseudomonas putida), 로도코커스 sp. B7740(Rhodococcus sp. B7740), 및 스트렙토미세스 항생제(Streptomyces antibioticus)로 이루어지는 군에서 선택된 1종 이상의 미생물을 유효성분으로 포함하는 저색소침착증 예측용 바이오마커 조성물, 이를 이용한 키트 및 이를 이용한 정보제공방법에 관한 것이다.

一种提高重组蛋白热稳定性和溶解度的双功能蛋白标签及基于其的产品和应用

Resumen de: CN120757621A

本发明公开了一种提高重组蛋白热稳定性和溶解度的双功能蛋白标签及基于其的产品和应用，属于蛋白工程技术领域。该双功能标签的氨基酸序列SEQ ID NO:1所示，具有70%‑95%以上的相似度，其氨基酸序列变异主要存在但不限于二级结构位置。该双功能标签可在目的蛋白的N端或C端使用，适用于原核表达系统中融合蛋白的表达和纯化。本发明通过理性设计改造天然蛋白的疏水性区域，解决了现有蛋白标签分子量大、易遮蔽目标蛋白活性结构域、标签切除后残留非天然氨基酸可能破坏蛋白正确折叠等技术问题，显著提升融合蛋白在大肠杆菌表达系统中的可溶性及热稳定性，适用于包涵体蛋白的功能性表达与工业化应用。

サルモネラの検出方法

Resumen de: JP2025149945A

【課題】 本発明が解決しようとする課題は、サルモネラの検出を、血清型横断的かつ他の菌種とは特異的に行うことである。【解決手段】サルモネラの加熱死菌体とアジュバントを含む抗原液を、ＢＡＬＢ／ｃマウスに接種した。接種後、マウスの脾細胞またはリンパ節細胞とＰ３Ｕ１とを融合させてハイブリドーマを作製し、その中から、サルモネラ特異性を示すモノクローナルＩｇＧ抗体を産生するハイブリドーマを選抜し、優れたサルモネラ特異性抗体を産生する３つのハイブリドーマを取得することに成功した。次いで、これらのモノクローナル抗体を用いて、サンドイッチＥＬＩＳＡにより複数血清型のサルモネラおよび他の菌種の検出を行ったところ、血清型横断的にサルモネラを検出し、他の菌種を検出しないという結果を得た。【選択図】図１

Extract of herbal composition as antimicrobial and/or anti-biofilm agent

Resumen de: CN120732933A

Described herein is an extract of a herbal composition comprising at least two different dry plants, the compounds are useful as antimicrobial and/or antibiotic film agents in the treatment or prevention of microbial infections caused by bacteria (e.g., Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Streptococcus, or Staphylococcus) or by fungi, as well as in the treatment or prevention of microbial infections caused by bacteria (e.g., Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Streptococcus, or Staphylococcus). It has been found that in such an extract, the active ingredients exert their biological effects in a synergistic manner. The extracts may form active ingredients of food supplements, nutraceuticals, pharmaceutical or cosmetic compositions or functional foods or food additives. Also described herein are methods for preparing the extracts.

Enterococcus lactis NSK220, microbial inoculum and application

Resumen de: CN120738054A

The invention belongs to the technical field of food microorganisms, and particularly relates to an enterococcus lactis NSK220 strain, a microbial agent and application of the enterococcus lactis NSK220 strain. The invention provides the enterococcus faecium NSK220, the clearance rate of the enterococcus faecium NSK220 on DPPH is very high, the serum SOD enzyme activity can be improved by supplementing the strain NSK220 in diet, and it is indicated that the oxidation resistance of the strain NSK220 is high. Meanwhile, the enterococcus faecium NSK220 has relatively strong self-cohesion and surface hydrophobicity, which indicates that the strain has strong colonization ability and is beneficial to long-term play of probiotic functions in intestinal tracts; the strain NSK220 can also improve the feed intake and kidney function of animals, and is beneficial to the growth and body health of the animals. The enterococcus faecium NSK220 has an inhibition effect on escherichia coli, staphylococcus aureus, salmonella, pseudomonas aeruginosa and listeria monocytogenes.

一种亚单位疫苗及其制备方法与应用

Resumen de: CN120737217A

本发明提供了一种亚单位疫苗及其制备方法与应用。所述疫苗包括式（1）的融合蛋白，D0a‑D1a‑Linker‑HA‑Linker‑D1b‑D0b（1）其中，D0a和D0b分别为细菌鞭毛蛋白FliC的D0结构域或其功能性片段的N端部分和C端部分，D0a和D0b组成细菌鞭毛蛋白FliC的D0结构域或其功能性片段；D1a和D1b分别为细菌鞭毛蛋白FliC的D1结构域或其功能性片段的N端部分和C端部分，D1a和D1b组成细菌鞭毛蛋白FliC的D1结构域或其功能性片段；HA为流感病毒HA蛋白的抗原表位或其功能性片段，linker为连接子，‑为肽键或其他化学键。可以用于流感病毒的净化工作，安全有效，具有较高的应用价值。

A PROCESS FOR PREPARING LACTOBACILLUS-BASED RECOMBINANT VACCINE CANDIDATE AGAINST MULTIPLE SALMONELLA SEROVAR IN POULTRY

Resumen de: WO2025202803A1

The process comprises providing a recombinant vaccine construct, wherein said construct comprises genetically modified Lactobacillus plantarum NC8 as a live vector; modifying the genetic structure of said Lactobacillus plantarum NC8 to express conserved Salmonella antigens, including PagN, SopE2, and FliC, anchored by a ptrk 892 backbone with a constitutive promoter, phosphoglycerate mutase (PGM); incorporating Signal Lp_2145 and cAM12 Anchor sequences into said genetic construct to enhance surface expression of recombinant proteins on Lactobacillus plantarum NC8; administering said recombinant vaccine orally to poultry, leveraging the probiotic properties of Lactobacillus plantarum NC8 for effective colonization of the poultry gastrointestinal tract; inducing a prolonged and intensified immune response by ensuring sustained high-level expression of target antigens through the utilization of the robust constitutive promoter, phosphoglycerate mutase (PGM); and optimizing immunogenicity through the surface expression of recombinant proteins on Lactobacillus plantarum NC8, fostering a robust and precisely targeted immune response.

FIMH INHIBITING COMPOSITIONS AND METHODS OF USE THEREOF

Resumen de: US2025304662A1

Among the various aspects of the present disclosure is the provision of FimH inhibiting compositions and methods of use thereof. FimH inhibiting compositions that target and inhibit FimH, including monoclonal antibodies, are described. Methods of identifying FimH inhibiting antibodies are also described. Further, a method of treating bacterial infections, including urinary tract infections, is described.

ALTERING MICROBIAL POPULATIONS & MODIFYING MICROBIOTA

Resumen de: EP4623922A2

The invention relates to guided nucleases, CRISPR/Cas systems, crRNAs, single gRNAs, vectors, methods and pharmaceutical compositions, for example for targeting sporulating bacteria, or for targeting C difficile, Salmonella, E coli or Streptococcus.

一种基因工程菌及其在抗革兰氏阴性菌感染中的应用

Resumen de: CN120718821A

本发明公开了一种基因工程菌及其在抗革兰氏阴性菌感染中的应用。所述基因工程菌为在出发菌株中利用细菌表面展示系统过表达菌毛黏附素、肠道消化酶识别位点和细菌素。本发明的基因工程菌能在肠道中肠激酶的作用下，释放有活性的细菌素并暴露出菌毛黏附素，并实现杀灭肠道内的鼠伤寒沙门氏菌，及预防鼠伤寒沙门氏菌的再次定植的效果。

Colloidal gold immunochromatography test paper for rapidly detecting pigeon type I paramyxovirus and preparation method of colloidal gold immunochromatography test paper

Resumen de: CN120721964A

The invention discloses colloidal gold immunochromatography test paper for rapidly detecting pigeon I-type paramyxovirus and a preparation method of the colloidal gold immunochromatography test paper. Viral diseases of pigeons, especially pigeon plague virus and pigeon adenovirus, are rapidly detected on the basis of a newly developed test strip, and the operation is simple and convenient; according to the design of antigens and antibodies of the pigeon plague virus and the pigeon adenovirus, rapid detection of the pigeon plague virus and the pigeon adenovirus can be conveniently carried out, and the obtained test strip is high in specificity and high in sensitivity. The pigeon I-type paramyxovirus detection kit has the advantages that the pigeon I-type paramyxovirus detection kit does not have cross reaction with chicken infectious bronchitis, chicken egg drop syndrome, chicken Marek's disease, avian influenza, fowl adenovirus, infectious bursal disease, escherichia coli, salmonella and other samples, but can have cross reaction with Newcastle disease, so that the pigeon I-type paramyxovirus detection kit can be used for diagnosing pigeon I-type paramyxovirus and Newcastle disease.

Salmonella enteritidis tolerant strain phage, phage preparation and application thereof

Resumen de: CN120699917A

The invention belongs to the technical field of biology, and relates to a bacteriophage, in particular to a salmonella enteritidis tolerant strain bacteriophage, a bacteriophage preparation and application of the bacteriophage preparation. The preservation number of the bacteriophage is CCTCC (China Center for Type Culture Collection) No.M 20251313, and the bacteriophage is preserved in the China Center for Type Culture Collection on June 9, 2025. The bacteriophage sBR4p4 is separated by taking a bacteriophage BS3 tolerant strain BR4 as host bacteria, and the bacteriophage is easy to separate. Biological characteristic determination results show that the sBR4p4 cracking spectrum and temperature tolerance range are wide, the stability is good, and the in-vitro cracking duration is long. Whole genome sequencing analysis shows that the bacteriophage sBR4p4 does not contain virulence genes, drug-resistant genes and lyogenic genes; and a basis is provided for further large-scale production of medicines for preventing or treating salmonella enteritidis and escherichia coli infection.

包含用于在没有任何佐剂的情况下经由阳离子多糖纳米颗粒递送灭活的完整细菌的系统的疫苗组合物

Resumen de: MX2025009500A

The invention relates to the field of vaccine compositions. The invention more particularly relates to a prophylactic vaccine composition that is intended for mammals and birds and comprises a killed whole bacterium, said bacterium being covered with a cationic agent, in particular cationic nanoparticles.

Application of undaria pinnatifida fucoidin oligosaccharide in preparation of product for preventing/relieving intestinal inflammation induced by salmonella typhimurium

Resumen de: CN120695024A

The invention discloses application of undaria pinnatifida fucoidin oligosaccharide in preparation of a product for preventing/relieving intestinal inflammation induced by salmonella typhimurium, and belongs to the technical field of medicines. According to the application of the undaria pinnatifida fucoidin oligosaccharide in preparation of the salmonella typhimurium bacteriostatic agent, the molecular weight of the undaria pinnatifida fucoidin oligosaccharide is 4000-4500 Da, and main monosaccharides of the undaria pinnatifida fucoidin oligosaccharide are Man, Gal and Fuc. The undaria pinnatifida fucoidin oligosaccharide has a protection effect on liver and spleen organ injury, can improve the physical sign performance of the liver and spleen organ injury, relieve inflammation symptoms, effectively inhibit invasion of pathogenic bacteria, better protect colon tissues from serious damage and protect organs from pathological damage.

RECOMBINANT ATTENUATED SALMONELLA VACCINES (RASVS) AGAINST INFECTIONS BY AVIAN PATHOGENIC ESCHERICHIA COLI (APEC)

Resumen de: US2025295747A1

The present disclosure provides compositions and methods for making and using Salmonella recombinant bacteria that express LPS O78 antigen and/or virulence factors of avian pathogenic Escherichia coli (APEC) as vaccines to prevent colibacillosis infections.

IGY ANTIBODY COMPOSITIONS AND METHODS FOR TREATING AVIAN SPECIES

Resumen de: WO2025199547A1

A composition and method for therapeutic or prophylactic treatment of birds includes a mixture of IgY antibodies derived from one or more eggs laid by one or more avian species, wherein each of the one or more avian species have been vaccinated with one or more of the plurality of antigens. The mixture of IgY antibodies can be combined with a protective material that includes colostrum.

IgY Antibody Compositions and Methods for Treating Avian Species

Resumen de: US2025295770A1

A composition and method for therapeutic or prophylactic treatment of birds includes a mixture of IgY antibodies derived from one or more eggs laid by one or more avian species, wherein each of the one or more avian species have been vaccinated with one or more of the plurality of antigens. The mixture of IgY antibodies can be combined with a protective material that includes colostrum.

RECOMBINANT ATTENUATED SALMONELLA VACCINES (RASVS) AGAINST INFECTIONS BY AVIAN PATHOGENIC ESCHERICHIA COLI (APEC)

Resumen de: WO2025199081A1

The present disclosure provides compositions and methods for making and using Salmonella recombinant bacteria that express LPS O78 antigen and/or virulence factors of avian pathogenic Escherichia coli (APEC) as vaccines to prevent colibacillosis infections.

IMMUNOENHANCING SALMONELLA STRAIN FOR TREATMENT OF CANCER AND USE THEREOF

Nº publicación: EP4621058A1 24/09/2025

Solicitante:

CNCURE CO LTD [KR]
CNCure Co., Ltd

Resumen de: EP4621058A1

The present invention relates to a DNA construct including: a gene encoding a flagellin protein; and a gene encoding an immunoenhancer (adjuvant) protein. For effective cancer therapy by selectively killing only cancer cells, an attenuated Salmonella strain according to the present disclosure is designed to produce immunogenic substances in cancer tissue to induce a strong anti-cancer immune response, whereby tumor sizes in metastatic cancers as well as primary cancers can be significantly inhibited. Thus, the strain can be advantageously used in a prophylactic or therapeutic composition for improving survival rates.