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CONTINUOUS FEED, HORIZONTAL WATER SPRAY SHELL EGG PASTEURIZATION SYSTEM

Resumen de: US2025169515A1

A continuous feed, shell egg pasteurization system and method uses sprayed heated water. The unpasteurized shell eggs are conveyed and rotated through a horizontal tunnel. An overlapping pattern of heated water is sprayed downward onto the rotating shells eggs. Verified testing shows that the shell eggs achieve a statistical 5 log reduction of Salmonella enteritidis that may have been present in the yolk in the respective unpasteurized shell egg, while at the same time are not overcooked and have little to no whitening of the albumen.

METHODS FOR TREATING INFECTIVE DISEASES

Resumen de: CN120051271A

The present invention provides a method of treating an infectious disease. The method comprises the step of administering to a subject in need thereof an effective amount of (i) a polymer-flavonoid conjugate, (ii) a flavonoid oligomer, or (iii) a micelle having a shell formed from one or more polymer-flavonoid conjugates or one or more flavonoid oligomers, or a combination thereof and having an agent encapsulated within the shell. The methods of the present invention can effectively treat viral infections, such as, for example; severe acute respiratory syndrome coronavirus (SARS-CoV), enterovirus, HIV, hepatitis B virus, MERS-CoV, influenza virus, dengue fever virus, respiratory syncytial virus, hepatitis C virus, monkey pox virus, human papilloma virus, methicillin-resistant staphylococcus aureus, pseudomonas, tuberculosis, bacillus anthracis, tetanus, streptococcus pneumoniae, meningococcus, escherichia coli, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, , Legionella genus, Neisseria gonorrhoeae, Neisseria meningitidis, and Salmonella genus.

细菌转染

Resumen de: AU2023361317A1

The present invention relates to a modified live attenuated Gram-negative bacteria, wherein said bacteria has been modified in such a way that RNA molecules can be safely and efficiently delivered to target eukaryotic cells. As such, the present invention relates a to a bacterial delivery system and various uses and methods thereof.

Monoclonal antibody for resisting pathogenic campylobacter and application thereof

Resumen de: CN120040584A

The invention discloses a monoclonal antibody CJ116 for resisting pathogenic campylobacter and an application of the monoclonal antibody CJ116. In the monoclonal antibody CJ116, the amino acid sequence of a heavy chain variable region is as shown in SEQ ID NO.1, and the amino acid sequence of a light chain variable region is as shown in SEQ ID NO.5. The CJ116 monoclonal antibody has high affinity and high specificity, the detection sensitivity of the CJ116 monoclonal antibody to campylobacter jejuni and campylobacter colons can reach 10 < 2 > CFU/mL, and the detection sensitivity of the CJ116 monoclonal antibody to campylobacter fetus, campylobacter upsackii and campylobacter seagull is 10 < 3 > CFU/mL. However, the enteropathogenic bacteria have no cross reaction with other common enteropathogenic bacteria such as pathogenic Escherichia coli, salmonella enteritidis, yersinia enterocolitica, Shigella flexneri, Shigella baumannii and Staphylococcus aureus.

Enterococcus faecium capable of simultaneously degrading three mycotoxins and application of enterococcus faecium

Resumen de: CN120041351A

The invention discloses enterococcus faecium capable of degrading three mycotoxins at the same time and application of the enterococcus faecium, and belongs to the technical field of microorganisms. The enterococcus faecium JBHBIO-BSC1 disclosed by the invention is preserved in the China Center for Type Culture Collection, and the preservation number of the enterococcus faecium JBHBIO-BSC1 is CCTCC (China Center for Type Culture Collection) NO: M 20241918. The enterococcus faecium disclosed by the invention has the most remarkable characteristic that the enterococcus faecium has an obvious degradation effect on the aflatoxin, the vomitoxin and the zearalenone, and the degradation rates of the aflatoxin, the vomitoxin and the zearalenone can respectively reach 74.67%, 85.83% and 82.12%. The enterococcus faecium disclosed by the invention is natural, safe, acid-resistant, alkali-resistant and high-temperature-resistant, and has relatively strong organic acid production capacity. In addition, the strain has an obvious inhibition effect on escherichia coli, salmonella and staphylococcus aureus, and is expected to be applied to the fields of food processing and microbial pharmacy.

Wide-splitting-spectrum salmonella typhimurium bacteriophage, bacteriophage composition thereof and application of bacteriophage composition

Resumen de: CN120041399A

The invention discloses a wide-splitting-spectrum salmonella typhimurium bacteriophage, a bacteriophage composition and application thereof, the bacteriophage is named as salmonella bacteriophage PC817 which is preserved in China General Microbiological Culture Collection Center on August 16, 2024, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.9687. The invention further discloses a preparation method of the wide-splitting-spectrum salmonella typhimurium bacteriophage. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection The bacteriophage PC817 is a salmonella typhimurium bacteriophage with a wide lysis spectrum, has the characteristic of high lysis rate for different types of salmonella, and can be used for preparing drugs for treating or preventing salmonellosis, environment disinfectants, feed additives, drinking water additives, food preservatives, detection kits and the like. While salmonella typhimurium infection is solved, the problems of antibiotic residues and pathogen drug resistance caused by use of antibiotics are avoided. The bacteriophage and the composition thereof can provide a more effective scheme for prevention and control of pigeon salmonellosis.

ＳＡＬＭＯＮＥＬＬＡ ＥＮＴＥＲＩＣＡを制御するためのバクテリオシン

Resumen de: US2023406892A1

The present invention relates to bacteriocins for control of Salmonella enterica (salmocins). The bacteriocins are derived from Salmonella. The salmocins can be expressed in plants and can be used in a method of preventing or reducing infection or contamination of an object with Salmonella.

Method for analyzing composition of human microbiota based on blood and uses thereof

Resumen de: KR20250073901A

본 발명은 혈액 기반 인체 마이크로바이오타 조성 검사 방법 및 이의 용도에 관한 것으로, 보다 구체적으로는 환자 유래 단리된 생물학적 시료를 384종의 인체 장내미생물 항원으로 이루어진 마이크로어레이에 처리하는 단계를 포함하는 질환 관련 치료제 후보 장내미생물의 스크리닝 방법 및 상기 스크리닝 방법을 통해 선발된 질환 관련 치료제 후보 장내미생물에 관한 것이다.

Salmonella longtail bacteriophage and application thereof

Resumen de: CN120025989A

The invention discloses a salmonella longtail bacteriophage and application thereof, the bacteriophage is salmonella bacteriophage YZA15 and is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO: M 20242878, the preservation date is December 23, 2024, and the preservation address is Wuhan. The salmonella bacteriophage YZA15 disclosed by the invention is good in stability, has no toxic drug-resistant genes, has splitting activity on main epidemic serotypes (mouse typhoid, enteritis and pullorum disease) of salmonella, is relatively specific in host range, short in incubation period and high in filial generation bacteriophage outbreak amount, can be applied to links of livestock breeding, slaughtering, processing and breeding, and has a broad application prospect. And a safe and efficient biological antibacterial product is provided for livestock and poultry environments and products thereof.

Salmonella identification culture medium and preparation method thereof

Resumen de: CN120026080A

The invention provides a salmonella identification culture medium and a preparation method thereof. The salmonella identification culture medium comprises the following components: a basic substrate, a sugar agent, a chromogenic substrate and a selective inhibitor. The chromogenic substrate comprises sodium thiosulfate and ammonium ferric citrate. The selective inhibitor is prepared from sodium sulfite, lauryl sodium sulfate, 7-ethyl-2-methyl-4-undecyl sodium sulfate and vancomycin. According to the salmonella identification culture medium, the identification efficiency of salmonella can be effectively improved, and the identification accuracy of salmonella is improved.

Chitosan coupled polypeptide and preservative film prepared from chitosan coupled polypeptide

Resumen de: CN120025401A

The invention provides chitosan coupled polypeptide and a preservative film prepared from the chitosan coupled polypeptide, active peptide used for preparing the preservative film has good antibacterial activity on escherichia coli, salmonella, staphylococcus aureus and drug-resistant escherichia coli, and the amino acid sequence of the active peptide is SEQ ID NO: 1. The preservative film prepared by coupling the active peptide and the chitosan provided by the invention has remarkable antibacterial activity, also has good resistance to drug-resistant strain drug-resistant escherichia coli, is good in mechanical property, air permeability, moisture penetrability and stability, and is suitable for being used in the fields of food preservation, medical dressing and the like.

Phage, composition and application

Resumen de: CN120025987A

The invention relates to the field of microorganisms, and provides a bacteriophage, a composition and application of the bacteriophage, and the preservation number of the bacteriophage is CCTCC M 2024569. The invention discloses a bacteriophage capable of splitting multiple salmonella and escherichia coli O157: H7, and a bacteriophage composition comprising the bacteriophage. The effective components of the bacteriophage composition comprise the bacteriophage or the bacteriophage composition. The bacteriophage provided by the invention has good biological characteristics, does not contain virulence genes and antibiotic resistance genes, and is wide in host spectrum and strong in splitting property. The bacteriophage preparation provided by the invention can be used as a biological prevention and control preparation to control infection of salmonella and escherichia coli O157: H7 antibiotic-resistant strains in food.

Application of dephosphorylated NDP52 protein in preparing medicine for controlling salmonella infection

Resumen de: CN120025421A

The invention belongs to the technical field of gene engineering, and particularly relates to application of dephosphorylated NDP52 protein in preparation of salmonella infection control drugs. The technical scheme of the invention is as follows: a protein NDP52S162A with an amino acid sequence of SEQ ID No.1, a gene for coding the protein and with a nucleotide sequence of SEQ ID No.2, a recombinant vector, a transgenic cell line or a recombinant virus containing the gene, and a substance capable of realizing dephosphorylation of the NDP52 protein are provided; the invention also discloses application of the gene, the protein and a substance for realizing dephosphorylation of the NDP52 protein in preparation of salmonella infection control medicines, and experiments in mice show that the NDP52 protein and the 162-site serine dephosphorylation thereof promote removal of salmonella in the liver of the mice and play a role in controlling salmonella infection.

SELF-ASSEMBLING PROTEIN NANOPARTICLES ENCAPSULATING IMMUNOSTIMULATORY NUCLEID ACIDS

Resumen de: US2025161423A1

The present invention relates to self-assembling protein nanoparticles encapsulating immunostimulatory nucleid acids. Furthermore, the invention relates to the use of such nanoparticles for vaccination.

LYTIC PHAGE WITH HIGH SPECIFICITY TOWARDS PATHOGENIC ESCHERICHIA COLI

Resumen de: US2025164483A1

Herein are presented methods for incorporating a novel bacteriophage, CAM-21, that exhibits lytic activity against pathogenic E. coli, into food, food packaging, films, and quantum dot-based biosensors. The bacteriophage contains no genes associated with toxins, virulence factors, antibiotic resistance, lysogeny, or allergens. Methods are provided for encapsulating the bacteriophage with proteins and/or carbohydrate materials to enhance stability. Various films in which the bacteriophage are incorporated, and which similarly exhibit suppression of pathogenic E. coli, are also provided. Additionally, when conjugated with N-doped graphene quantum dots, CAM-21 enables biosensor development for E. coli detection in food products through altered photoluminescence emission spectra, and methods for making and using such biosensors are provided.

METHOD FOR PREPARING FREEZING SHOCK-TREATED MONOCYTES OR MACROPHAGES LOADED WITH ATTENUATED SALMONELLA AND USE THEREOF

Resumen de: WO2025102801A1

Provided are a method for preparing freezing shock-treated monocytes or macrophages loaded with attenuated Salmonella and the use thereof. The method comprises: loading engineering-modified attenuated Salmonella in monocytes or macrophages, and performed a freezing shock treatment on the monocytes or macrophages loaded with the strain by means of liquid nitrogen. The method for preparing the freezing shock-treated immune cells loaded with the strain is simple and easy to operate. The source problem of the monocytes or macrophages loaded with the engineering-modified attenuated Salmonella is solved, and the method has good application prospects. According to the strategy, by means of avoiding exposure and heterologous stimulation of bacteria, the biological safety of bacteria-based anti-tumor treatment is improved; and the anti-tumor immune response is promoted by means of the high titer of an intratumoral strain, so that a stronger anti-tumor efficacy is obtained.

LACTIPLANTIBACILLUS PLANTARUM GOLDGUT-LP618 HAVING FUNCTION OF RESISTING SALMONELLA INFECTION AND APPLICATION THEREOF

Resumen de: AU2024256082A1

The present invention relates to the technical field of probiotics, in particular to a Lactiplantibacillus plantarum GOLDGUT-LP618 having a function of resisting Salmonella infection and application thereof. The Lactiplantibacillus plantarum GOLDGUT-LP618 provided by the present invention is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 28399. The strain has high resistance to acid and bile salt and can adapt to the digestive tract environment. The strain can inhibit Salmonella infection, relieve diarrhea, inflammation and intestinal damage caused by Salmonella infection, and promote intestinal health. The strain can be used for preparing functional foods, dietary supplements or drugs that can resist Salmonella infection and improve diarrhea and inflammation caused by Salmonella infection, and has good application prospects.

RAPID DETECTION METHOD FOR SALMONELLA USING POLYDIACETYLENE-BASED COLOR-CHANGING LIPOSOME PARTICLES

Resumen de: KR20250069736A

폴리디아세틸렌 기반 색 변이 리포좀 입자를 이용한 살모넬라균의 신속한 검출방법을 개시한다. 상기 살모넬라균의 신속 검출방법은 (1) 살모넬라균을 박테리아 단백질 추출 시약에 용해시켜 살모넬라균 조각으로 추출하여 살모넬라균을 전처리하는 단계; (2) 전처리된 살모넬라균과 리포좀 입자를 혼합하여 혼합액을 제조하는 단계; 및 (3) 상기 혼합액의 색변환을 분석하여 살모넬라균을 검출하는 단계;를 포함하고, 살모넬라균을 신속하게 검출할 수 있다.

Basa fish Nat10 antibacterial peptide as well as coding sequence, application and preparation method thereof

Resumen de: CN120004982A

The invention discloses a Basa fish Nat10 antibacterial peptide as well as a coding sequence, application and a preparation method thereof, an antibacterial peptide precursor gene sequence is screened and identified from a Basa fish multi-omics data set based on a multi-omics method and biological big data, and an amino acid sequence of the Basa fish Nat10 antibacterial peptide as shown in SEQ ID NO: 3 is obtained through translation design; activity simulation prediction and in-vitro experiments prove that the Basa fish Nat10 antibacterial peptide has an inhibiting effect on growth activity of streptococcus agalactiae, salmonella enteritidis, staphylococcus aureus, vibrio anguillarum and escherichia coli, and particularly has the best antibacterial effect on staphylococcus aureus at the concentration of 2.5 mu mol/L. The compound can be used for preparing various sterilization preparations including bacteriostatic agents, medicines or additives and the like, and has good practical value and social and ecological benefits.

Application of salinomycin in prevention and treatment of pathogenic microorganisms

Resumen de: CN119999693A

The invention discloses application of salinomycin to prevention and treatment of pathogenic microorganisms. According to the present invention, the compound provides significant inhibition activity for agricultural fungi such as Sclerotinia sclerotiorum, Botrytis cinerea, Pyricaria oryzae, Moniliniafacticola ACCC 36263, food-borne bacteria such as Escherichia coli O157: H7ATCC 35150, Bacillus cereus BN CC 103930, Listeria monocytogenes ATCC 19115 and Salmonella enteritidis ATCC 14028, and further provides a certain inhibition effect on other agricultural pathogenic bacteria, such that the compound is expected to be developed into the one type of the compound,

Basa fish Mob1b antibacterial peptide as well as coding sequence, application and preparation method thereof

Resumen de: CN120004981A

The invention discloses a Basa fish Mob1b antibacterial peptide as well as a coding sequence, application and a preparation method thereof, an antibacterial peptide precursor gene sequence is screened and identified from a Basa fish multi-omics data set based on a multi-omics method and biological big data, and an amino acid sequence of the Basa fish Mob1b antibacterial peptide as shown in SEQ ID NO: 3 is obtained through translation design; activity simulation prediction and in-vitro experiments prove that the Basa fish Mob1b antibacterial peptide has an inhibition effect on growth activity of streptococcus agalactiae, salmonella enteritidis, staphylococcus aureus, vibrio anguillarum and escherichia coli; the compound can be used for preparing various antibacterial agents, especially gram-positive bacteria streptococcus agalactiae, gram-negative escherichia coli staphylococcus aureus and vibrio anguillarum, can show an antibacterial effect when the concentration is 2.5 mu mol/L, can achieve the best antibacterial effect when the concentration is 5 mu mol/L, can be used for preparing various antibacterial agents and the like, and has good practical value and social and ecological benefits.

IMMUNOENHANCING SALMONELLA STRAIN FOR TREATMENT OF CANCER AND USE THEREOF

Resumen de: AU2023382983A1

The present invention relates to a DNA construct including: a gene encoding a flagellin protein; and a gene encoding an immunoenhancer (adjuvant) protein. For effective cancer therapy by selectively killing only cancer cells, an attenuated Salmonella strain according to the present disclosure is designed to produce immunogenic substances in cancer tissue to induce a strong anti-cancer immune response, whereby tumor sizes in metastatic cancers as well as primary cancers can be significantly inhibited. Thus, the strain can be advantageously used in a prophylactic or therapeutic composition for improving survival rates.

DNA CONSTRUCT FOR EXPRESSING SIGNALING SUBSTANCE FOR CANCER TREATMENT, AND CANCER TREATMENT METHOD USING SAME

Resumen de: WO2025100987A1

Cyclic dinucleotides, which are a type of signaling substance for cancer treatment, are secondary messengers of intracellular events initiated by GPCR activation and act as a stimulator of interferon genes (STING), and thus can have a significant effect on tumor suppression. Meanwhile, when cancer occurs in a subject, angiogenesis and cell growth proceed at a very high rate in the body, and thus an oxygen-deficient environment is created due to incomplete angiogenesis in cancer tissues, which may be very suitable for the proliferation of anaerobic bacteria such as Salmonella sp. strains or E. coli. Therefore, the present invention relates to a DNA construct into which is introduced a gene for an enzyme that synthesizes a signaling substance for cancer treatment, e.g., a cyclic dinucleotide, or to a strain transformed with a vector comprising the DNA construct. The DNA construct or the strain transformed with a vector comprising the DNA construct, according to the present invention, targets cancer in a subject and then secretes C-di-AMP or C-di-GMP synthase in the surrounding environment of the cancer, and thus can very effectively prevent or treat cancer and, at the same time, can diagnose cancer in real time.

Positively charged silicon shell modification-based magnetic fluorescent probe, preparation method and application

Resumen de: CN119979149A

The invention discloses a magnetic fluorescent probe based on positive silicon shell modification as well as a preparation method and application of the magnetic fluorescent probe. The probe adopts superparamagnetic Fe3O4 nanoparticles as an inner core and provides magnetic enrichment capacity; a cationic polymer electrostatically adsorbs multiple layers of quantum dot particles to serve as a fluorescent interlayer, and a fluorescent signal capable of being used for quantitative detection is provided; and a layer of aminated silicon dioxide shell with strong positive electricity is also arranged on the surface, so that the broad-spectrum capturing capability on bacteria with negative electricity is provided. By taking pseudomonas aeruginosa, streptococcus pneumoniae and salmonella typhimurium as examples, rapid enrichment and broad-spectrum quantitative analysis of the pseudomonas aeruginosa, streptococcus pneumoniae and salmonella typhimurium in a sample can be realized, a magnetic enrichment effect and multiple signal amplification of multiple layers of fluorescent quantum dots are realized, matrix interference in practical application is eliminated, and the detection sensitivity is high. The method has great potential in the aspects of high sensitivity and clinical monitoring of bacterial infection or pollution.

Primer probe composition and kit for simultaneously detecting 17 pathogenic microorganisms in food

Nº publicación: CN119979776A 13/05/2025

Solicitante:

SHENZHEN ACAD OF METROLOGY & QUALITY INSPECTION
SHENZHEN ZIJIAN BIO TECH CO LTD
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\u6DF1\u5733\u5E02\u6893\u5065\u751F\u7269\u79D1\u6280\u6709\u9650\u516C\u53F8

Resumen de: CN119979776A

The invention discloses a primer probe composition and a kit for simultaneously detecting 17 pathogenic microorganisms in food. The primer probe composition is used for detecting salmonella, staphylococcus aureus, clostridium perfringens, escherichia coli O157, listeria monocytogenes, vibrio parahaemolyticus, bacillus cereus, vibrio cholerae, campylobacter jejuni, clostridium botulinum, proteusbacillus vulgaris, group A rotavirus, norovirus type GI, norovirus type GII and the like. The invention relates to a primer and probe combination for astrovirus, fiveleaf virus and enteric adenovirus. The primer and probe composition disclosed by the invention can be used for efficiently detecting 17 pathogenic microorganisms in food, has the advantages of simple and accurate judgment result, high sensitivity, strong specificity and high precision, and can meet the requirement of food detection industry on rapid detection.