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Method for synchronously and quickly detecting various mycotoxins by competitive immunochromatography based on microbial nano-enzyme tag and application thereof

Resumen de: CN121276046A

The invention discloses a method for synchronously and quickly detecting various mycotoxins by competitive immunochromatography based on a microbial nano-enzyme label and application, and the method can be used for simultaneously and accurately detecting various common mycotoxins, including aflatoxin B1, zearalenone and fumonisins B1, by utilizing the enhanced catalytic effect mediated by the microbial nano-enzyme label. The surface of Escherichia coli is coated with a SiO2 interlayer so as to improve the structural stability and the solution dispersity, and meanwhile, the surface of Escherichia coli is coated with a layer of catalytic shell composed of Au (at) Pt nanoparticles so as to generate excellent colorimetric and catalytic performance. According to the application, ESi-Au-Pt is introduced into a nano enzyme-ICA system, so that the sensitivity and accuracy of on-site analysis of a complex sample are remarkably improved, the limit of detection (LOD) of synchronous detection of target fungaltoxin is reduced to 6.1/1.6/5.4 pg/mL, the detection range spans 4-5 orders of magnitude, in addition, the established method shows excellent accuracy and stability in a complex matrix, and the method can be applied to the field analysis of fungaltoxin. The method shows a huge potential in the field-based real-time monitoring of small-molecule pollutants.

Multiplex PCR (Polymerase Chain Reaction) primer for simultaneously detecting eight respiratory tract pathogenic bacteria and application of multiplex PCR primer

Resumen de: CN121272080A

The invention belongs to the technical field of bacterium detection, and discloses a multiple PCR primer for simultaneously detecting eight respiratory tract pathogenic bacteria and application thereof. On the basis of a Luminex liquid phase chip technology, specific primers are designed aiming at eight common respiratory tract infection pathogenic bacteria including pseudomonas aeruginosa, staphylococcus aureus, streptococcus pneumoniae, klebsiella pneumoniae, acinetobacter baumannii, stenotrophomonas maltophilia, haemophilus influenzae and escherichia coli; the multiple PCR primers which are high in amplification efficiency, good in specificity and suitable for simultaneously detecting the eight respiratory tract pathogenic bacteria are screened out, the multiple PCR system and microsphere hybridization conditions are continuously optimized, the multiple PCR method for the eight respiratory tract pathogenic bacteria is successfully constructed by applying the Luminex xTAG technology, and the multiple PCR method can be applied to rapid detection of multiple pathogenic microorganisms.

Method and device for quantitatively detecting pathogenic bacteria and detecting sensitivity of antibacterial agent

Resumen de: CN121249844A

The invention relates to the technical field of biosensing, in particular to a pathogenic bacteria quantitative detection and antibacterial drug sensitivity detection method and device. Based on the relationship between the concentration of gelatin and the time when a to-be-detected solution passes through a detection interval from bottom to top, a pathogenic bacteria quantitative detection and antibacterial drug sensitivity detection method is obtained; according to the quantitative detection method for the pathogenic bacteria, on the basis of ensuring the sensitivity, the detection time is shortened, and staphylococcus aureus as low as 1 CFU/mL can be detected after incubation for 8 hours. The MIC value measured by the antibacterial drug sensitivity detection method is highly consistent with the result of the traditional gold standard method (broth microdilution method), but the required time is greatly shortened (only about 6 hours are needed, and the standard method needs 18-24 hours).

PCR kit for triple PCR detection of escherichia coli, salmonella enteritidis and riemerella anatipestifer and application of PCR kit

Resumen de: CN121249921A

The invention belongs to the technical field of triple PCR detection PCR kits, and particularly relates to a triple PCR detection PCR kit for escherichia coli, salmonella enteritidis and riemerella anatipestifer and application of the triple PCR detection PCR kit. According to the kit, specific primers are designed by taking an escherichia coli alkaline phosphatase gene, a salmonella enteritidis invasion protein A gene and a riemerella anatipestifer helicase gene as target genes, the specific primers comprise a primer pair aiming at the phoA gene, the sequence of an upstream primer phoA-F is 5 '-TACAGGTACTGCGGGCTTATC-3', the sequence of a downstream primer phoA-R is 5 '-CTTACCGGGAATACATCACA-3', and the length of an amplified target fragment is 622bp; according to the primer pair for the invA gene, the sequence of an upstream primer invA-F is 5 '-AAAAGAAGGGTCGTCGTTAG-3', the sequence of a downstream primer invA-R is 5 '-GGAAGGTACTGCCAGAGGTC-3', the length of an amplified target fragment is 801 bp, and according to the primer pair for the dnaB gene, the sequence of an upstream primer dnaB-F is 5 '-GACGCTATATCATAGATTTAAC-3', the sequence of a downstream primer dnaB-R is 5 '-TCCACCGCTATATATTCTAG-3', and the length of the amplified target fragment is 431 bp.

Electrochemical sensing detection system and method based on bacterial nucleotide metabolism

Resumen de: CN121253627A

The invention relates to the technical field of water toxicity detection, in particular to an electrochemical sensing detection system and method based on bacterial nucleotide metabolism. Comprising the following steps: dispersing carboxylated multi-walled carbon nanotubes in N, N-dimethylformamide to prepare a dispersion liquid with a concentration of 0.5 to 1 mg/mL; adding ZnO nanoparticles, and performing ultrasonic treatment to obtain a mixed dispersion liquid; and dispensing the mixture on the surface of a pretreated glassy carbon electrode, and drying the glassy carbon electrode under an infrared lamp to obtain the nano-composite modified electrode. A three-electrode system formed by taking the nano-composite modified electrode as a working electrode, taking saturated calomel as a reference electrode and taking a platinum column as a counter electrode is connected to an electrochemical workstation; a guanine and xanthine mixed oxidation peak current generated by metabolism of Escherichia coli is used as a comprehensive toxicity effect index, and the toxicity of a target system is evaluated by monitoring the change of the mixed oxidation peak current. According to the invention, by utilizing the synergistic interaction of the nano composite material, the sensitive and rapid detection of the comprehensive toxicity of the water body is realized without adding a mediator.

Intestinal escherichia coli gene detection kit based on silicon-based micro-fluidic chip and detection method of intestinal escherichia coli gene detection kit

Resumen de: CN121227912A

The invention discloses an intestinal escherichia coli gene detection kit based on a silicon-based micro-fluidic chip and a detection method of the intestinal escherichia coli gene detection kit. The kit provided by the invention comprises a rapid hot start DNA polymerase, a PCR reaction mixed solution, a positive reference substance and a negative reference substance, the kit also comprises the following primers which take the intestinal escherichia coli fliC gene as a target gene, are designed by a PCR fluorescent probe method based on a micro-fluidic chip technical platform: an upstream primer F, a downstream primer R and a detection probe P, and the nucleotide sequences of the primers are shown as SEQ ID NO.1-3. According to the invention, the intestinal escherichia coli gene detection kit which is more comprehensive in detection effect, high in specificity, good in sensitivity, low in omission ratio, convenient, simple and easy to operate is provided.

Multiplex PCR (Polymerase Chain Reaction) method for rapidly detecting pathogenic bacteria of tobacco brown spot

Resumen de: CN121227938A

The invention discloses a multiplex PCR (polymerase chain reaction) method for quickly detecting pathogenic bacteria of tobacco brown spot, which is used for synchronously identifying two main pathogenic bacteria causing tobacco brown spot, namely alternaria tenuissima and alternaria alternata, and comprises the following steps: S1, extracting genome DNA (deoxyribonucleic acid): extracting genome DNA of standard strains of alternaria tenuissima and alternaria alternata as a detection template; the multiple PCR system has the beneficial effects that through system verification, it is confirmed that all primers only generate expected amplification products for target pathogenic bacteria, no cross amplification reaction exists between alternaria alternata and alternaria tenuissima, and it is indicated that the multiple PCR system has high interspecific specificity. The established detection method is high in stability, good in repeatability and suitable for standardized operation under different laboratory conditions. Compared with a traditional morphological identification or single gene sequencing method, the multiplex PCR technology has the advantages that the detection period is remarkably shortened, the operation is simple and convenient, time and labor are saved, the cost is low and the like. The method is suitable for rapid identification of standard strains.

Detection system for rapidly identifying drug resistance phenotypes of pathogenic bacteria based on machine learning in combination with MALDI-TOF MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry)

Resumen de: CN121237227A

The invention relates to the technical field of clinical microbiological detection, in particular to a detection system for rapidly identifying drug resistance phenotypes of pathogenic bacteria based on machine learning in combination with MALDI-TOF MS. According to the system, optimal detection conditions are screened out, clinically separated pathogenic bacteria and antibiotics are subjected to grouping co-incubation, and MALDI-TOF MS detection is carried out through dynamic sampling at multiple time points; preprocessing the obtained mass spectrum data, and calculating to obtain an enhanced dynamic relative growth (RBD-RG) feature vector matrix; a plurality of machine learning models are trained based on the matrix, and an optimal drug resistance phenotype prediction model is screened out through cross validation, so that the drug resistance phenotypes (sensitivity, mediation and drug resistance) of pathogenic bacteria are quickly and accurately judged. The whole detection process can be completed within 2 hours, the detection efficiency is remarkably improved, key technical support is provided for early-stage precise medication of infectious diseases, and improvement of prognosis of patients and restraint of infection spreading are facilitated.

Method for detecting mouse salmonella by using qPCR (quantitative polymerase chain reaction) technology instead of traditional sacrificial sentinel mouse

Resumen de: CN121227909A

The invention discloses a method for detecting mouse salmonella by using a qPCR (quantitative polymerase chain reaction) technology instead of a traditional sacrificial sentinel mouse, and a salmonella specific primer and probe combination comprises a forward primer with a sequence of 5 '-AGCGTACTGGAAAGGGAAAG-3', as shown in SEQ ID NO.1, a reverse primer with a sequence of 5 '-AGCGTACGAAAG-3', as shown in SEQ ID NO.2, a reverse primer with a sequence of 5 '-AGCGTACGAAGGAAAG-3', as shown in SEQ ID NO.3, the sequence of a reverse primer is 5-ATACCGCCAATAAAGTTCACAAAG-3 ', and the sequence of the reverse primer is as shown in SEQ ID NO. 2; the probe sequence of the probe is 5 '-FAM-CGTCACCTTGATAAACTCTATGCA-BHQ1-3', and the probe sequence of the probe is as shown in SEQ ID NO. 3. The method has the beneficial effects that a real-time fluorescent quantitative PCR method is established by utilizing the specific primer and the double-labeled fluorescent probe, so that rapid detection of salmonella pollution in facilities is realized; according to the method based on the environmental sample, use of additional animals is avoided, operation is easy and convenient, the result is accurate, and sensitivity and specificity are both superior to those of a traditional sentinel mouse method.

Method for synchronously detecting food-borne pathogenic bacteria based on multiple bacteriophage modified nano-enzyme

Resumen de: CN121231460A

The invention discloses a method for synchronously detecting food-borne pathogenic bacteria based on multiple bacteriophage modified nano enzyme, and belongs to the technical field of biology, a phage coated FeCoCe NF + TMB color development system is adopted to react with a solution to be detected, and the food-borne pathogenic bacteria in the solution to be detected are detected through the color development reaction; in the phage (at) FeCoCe NF + TMB color development system, a preparation method of the phage (at) FeCoCe NF comprises the following steps: immobilizing phages EhpYZU20, SalmpYZU54 and SapYZUH5 on a FeCoCe NF nano enzyme, so as to obtain the phage (at) FeCoCe NF. The preparation method of the phage (at) FeCoCe NF comprises the following steps of: immobilizing phages EhpYZU20, SalmpYZU54 and SapYZUH5 on the FeCoCe NF nano enzyme; according to the invention, synchronous visual detection of three food-borne pathogenic bacteria can be realized.

Detection method and application of mouse pathogenic bacteria

Resumen de: CN121227913A

The invention relates to a multiplex fluorescent quantitative PCR (Polymerase Chain Reaction) method for detecting mouse pathogenic bacteria, which can be used for simultaneously detecting four pathogens, namely salmonella, corynebacterium murine, pseudomonas aeruginosa and klebsiella pneumoniae.

Specific primer set of Pseudomonas aeruginosa and method for detecting Pseudomonas aeruginosa using the same

Nº publicación: KR20250178279A 29/12/2025

Solicitante:

대한민국농촌진흥청장

Resumen de: KR20250178279A

본 발명은 서열번호 1의 올리고뉴클레오티드 프라이머 및 서열번호 2의 올리고뉴클레오티드 프라이머를 포함하는, 슈도모나스 에르지노사(Pseudomonas aeruginosa) 검출용 프라이머 세트; 이를 포함하는 조성물; 이를 포함하는 키트; 및 이를 이용한 슈도모나스 에르지노사(Pseudomonas aeruginosa) 검출 방법에 관한 것이다.