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Tumor in-situ vaccine for relieving tumor immunosuppression as well as preparation method and application of tumor in-situ vaccine

Resumen de: CN122516344A

The invention discloses a tumor in-situ vaccine for relieving tumor immunosuppression as well as a preparation method and application of the tumor in-situ vaccine. The tumor in-situ vaccine comprises inactivated bacteria, heat shock protein and adenosine deaminase, wherein the heat shock protein and the adenosine deaminase are arranged in cell membranes of the inactivated bacteria; the inactivated bacteria are any one or two of inactivated escherichia coli (Escherichia coli) Nissle 1917 and inactivated attenuated salmonella typhimurium VNP 20009, and the inactivated bacteria are any one or two of inactivated escherichia coli (Escherichia coli) Nissle 1917 and inactivated attenuated salmonella typhimurium VNP 20009. The tumor in-situ vaccine provided by the invention can synergistically exert a natural immunologic adjuvant and metabolism regulation effect, effectively degrade adenosine and ROS in a tumor microenvironment, activate an immune system through Hsp, and inhibit tumor growth and metastasis.

Mucin-tannic acid packaged atractylodes macrocephala polysaccharide and lactobacillus casei compound as well as preparation method and application of mucoprotein-tannic acid packaged atractylodes macrocephala polysaccharide and lactobacillus casei compound

Resumen de: CN122516140A

The invention discloses a mucoprotein-tannic acid packaged atractylodes macrocephala polysaccharide and lactobacillus casei compound as well as a preparation method and application thereof, and relates to the technical field of biology. The mucoprotein-tannic acid packaged atractylodes macrocephala polysaccharide and lactobacillus casei compound is of a core-shell structure and sequentially comprises a probiotic core, an inner coating layer and an outer coating layer from inside to outside, the probiotics are lactobacillus casei; the inner coating layer is formed by cross-linking tannic acid and mucoprotein; the outer coating layer is rhizoma atractylodis macrocephalae polysaccharide. The mucoprotein-tannic acid packaged atractylodes macrocephala polysaccharide and lactobacillus casei compound provided by the invention can resist the severe environment of the digestive tract, improve the survival rate of probiotics, and perform targeted colonization on the intestinal tract to inhibit the invasion of salmonella. The feed additive can repair double barriers of intestinal tracts, regulate and control immune inflammation of organisms and recover growth performance of piglets, is free of drug resistance and drug residues, can safely prevent and control salmonella infection of the piglets, and is wide in breeding application prospect.

Antibacterial peptide and application thereof

Resumen de: CN122520746A

The invention belongs to the field of pharmacy, and relates to an antibacterial peptide and application thereof. The invention specifically discloses an antibacterial peptide with an amino acid sequence as shown in SEQ ID NO. 2. The antibacterial peptide can be used for remarkably inhibiting staphylococcus aureus with the preservation number of ATCC 29213 or ATCC 33591, enterococcus faecium with the preservation number of ATCC 700221, streptococcus suis with the preservation number of ATCC 43765, acinetobacter baumannii with the preservation number of ATCC 19606, pseudomonas aeruginosa with the preservation number of ATCC 27853, salmonella with the preservation number of ATCC 51741 and escherichia coli with the preservation number of ATCC 25922. The antibacterial peptide is good in safety and high in antibacterial activity and has application and popularization prospects.

Liquid chip nucleic acid detection kit and detection method for simultaneously detecting 10 experimental animal intestinal pathogens

Resumen de: CN122521874A

The invention discloses a liquid chip nucleic acid detection kit and a detection method for simultaneously detecting 10 experimental animal intestinal pathogens. The 10 pathogenic bacteria comprise salmonella typhimurium, Tyzz pathogen, klebsiella pneumoniae, pseudomonas aeruginosa, citrobacter rodent, helicobacter hepatae, klebsiella oxytoca, staphylococcus aureus, corynebacterium bovis and yersinia pseudotuberculosis. Primers are designed for specific target genes of various pathogenic bacteria, the 5'end of an upstream primer is connected with a TAG sequence, and the 5 'end of a downstream primer is labeled with biotin; after multiple PCR amplification, a product is hybridized with MagPlex-TAG microspheres with corresponding numbers, and a liquid phase chip system is used for detecting fluorescence signals. The method disclosed by the invention can be used for simultaneously detecting the 10 pathogenic bacteria in the same reaction system, has the advantages of high throughput, high sensitivity, high specificity, good repeatability and the like, and provides an efficient monitoring tool for quality control of experimental animal microorganisms.

大肠埃希氏杆菌感染的噬菌体疗法

Resumen de: US2017000831A1

0001 The present invention relates to bacteriophage therapy. More particularly, the present invention relates to novel bacteriophages having a high specificity against Escherichia coli strains, their manufacture, components thereof, compositions comprising the same and the uses thereof in phage therapy.

IN OVO VACCINATION OF GENETICALLY MODIFIED SALMONELLA VECTORED VACCINES

Resumen de: WO2026165563A1

Disclosed are methods for inoculating embryonated avian eggs for conferring protective immune responses against diseases caused by a diversity of bacterial, viral and parasite pathogens. According to certain aspects methods are disclosed that involve administering, in ovo, a safe inoculating dose of a Protective Immunity Enhanced Salmonella Vaccine (PIESV) strain, or derivative thereof, and optionally co-administering a safe inoculating dose of a live self-destructing attenuated adjuvant Salmonella strain (SDAAS), or derivative thereof.

COMPOSITIONS AND METHODS FOR ENHANCING ANIMAL FEED SAFETY AND CONTROLLING PATHOGENS

Resumen de: WO2026165105A1

The present inventive concept provides non-toxic compositions that include components selected from ethanol, polymers, polar aprotic solvents, water, organic acids, phospholipids, polyols, polysaccharides, phenols, plant extracts, organic salts, peroxide, and antibiotics. The compositions are designed as an enhancement or additive to animal feed additives, animal feedstuff, animal feed, or animal hydration sources. The compositions provide a safer alternative to traditional formaldehyde (e.g., aldehyde-based) compositions by incorporating preservative and antimicrobial agents into animal feed products. The compositions provided herein render animal feed highly resistant to recontamination by pathogenic bacteria such as Salmonella, Campylobacter, Streptococcus, Staphylococcus, E. coli, Clostridia, and Bacillus. Moreover, the compositions provide safer working conditions by eliminating workers' exposure to carcinogenic substances.

METHOD FOR IDENTIFYING A MULTI-PARAMETER PHENOTYPE OF MICROBIOTA

Resumen de: US20260227396A1

0000 A method for identifying a multi-parameter phenotype of microbiota. The method includes (i) providing a sample including microbiota, (ii) labeling the microbiota with multiple labels, each of which binds a phenotypic parameter of the microbiota, (iii) detecting an intensity of the labelled phenotypic parameters of single cells of the microbiota by flow cytometry, and (iv) segmenting the single cells into bins based on the intensities of detected phenotypic parameters, wherein the distribution of single cells in bins represents a multi-parameter phenotype of said microbiota. Also described is a system for identifying a multi-parameter phenotype of intestinal microbiota, a kit for identifying a multi-parameter phenotype of intestinal microbiota and methods for diagnosing a medical condition associated with microbiota, for example an inflammatory condition, such as an inflammatory bowel disease, in a subject.

BIOSENSOR MATERIALS

Resumen de: US20260227395A1

0000 The disclosure provides a three-dimensional (3-D) macroporous melamine foam membrane comprising an amine reactive group. The membrane foam can be used to perform ELISA assays for the detection of an analyte. Diffusion of the analyte through the different membrane layers is fast and homogeneous in all directions and suitable for detection of large volume of samples in low concentration of targets and multiple targets simultaneously in one integrated system with minimal interference from targets.

METHODS FOR PRODUCING HIGH-LEVEL SQUALENE

Resumen de: US20260226485A1

0000 Aspects of the present disclosure relate to modified yeast cells capable of producing increased quantities of squalene as compared to unmodified yeast cells and methods of using the same.

COMPOSITIONS AND METHODS FOR CONTROLLING PATHOGENS AND IMPROVING HYGIENE IN THE POULTRY AND LIVESTOCK INDUSTRIES

Resumen de: WO2026165108A1

The present inventive concept provides methods using non-toxic compositions that include components selected from ethanol, polymers, polar aprotic solvents, water, organic acids, phospholipids, polyols, polysaccharides, phenols, plant extracts, organic salts, peroxide, and peptide antibiotics designed as hygienic agents for the poultry and livestock industries. The compositions and methods provide a safer alternative to use of traditional formaldehyde (e.g., aldehyde-based) compositions by incorporating non-toxic preservation and antimicrobial agents to enhance biosecurity and sanitation in the livestock environments, and in particular, poultry production environments. The methods provided herein also control the growth of pathogenic bacteria such as Salmonella, Campylobacter, Streptococcus, Staphylococcus, E. coli, Clostridia, and Bacillus. Moreover, the methods and compositions provide safer working conditions by eliminating workers' exposure to carcinogenic substances.

一种胶体金免疫层析病菌检测系统及其检测方法

Resumen de: CN122506156A

本发明公开了一种胶体金免疫层析病菌检测系统及其检测方法，涉及免疫测定技术领域，包括：获取待测液体样本；利用毛细作用使所述待测液体样本沿所述试纸条层析，与所述胶体金标记抗体结合形成免疫复合物；若样本中存在目标病菌，则所述免疫复合物在迁移至所述检测线时被所述捕获抗体捕获并显色；同时，未结合的胶体金标记抗体在所述质控线处被所述二抗捕获并显色；当层析前沿到达所述吸水垫时，通过智能读取终端采集所述试纸条的显色图像；将所述显色图像上传至云端分析平台，基于深度学习模型对检测线与质控线的显色强度进行定量分析，输出目标病菌的浓度值及阴阳性判定结果；本发明提升了胶体金免疫层析病菌检测的准确性。

基于噬菌体展示技术筛选的沙门菌模拟抗原表位肽及应用

Resumen de: CN122502436A

本发明适用于微生物技术领域，提供了一种基于噬菌体展示技术筛选的沙门菌模拟抗原表位肽及应用，其中，该沙门菌模拟抗原表位肽能够与抗沙门菌的多克隆抗体特异性结合，其包括如序列表SEQ ID NO:1‑4所示的一种或多种氨基酸序列。本发明筛选的沙门菌模拟抗原表位肽能特异性结合抗沙门菌的多克隆抗体，显著诱导机体体液免疫和细胞免疫应答，并对多种沙门菌血清型及产肠毒素大肠杆菌可以产生交叉保护，攻毒存活率高，安全性好。本发明为由革兰氏阴性病原体引起的疾病的预防和治疗提供了一种全新的有效疫苗。

一种具有免疫增强功能的蛋白、其编码基因及应用

Resumen de: CN122502457A

本发明公开了一种具有免疫增强功能的蛋白、其编码基因及应用。本申请所述具有免疫增强功能的蛋白的氨基酸序列如SEQ ID NO.1所示。该蛋白取自鼠伤寒沙门菌鞭毛蛋白FliC的C端部分序列，不仅保留了鞭毛蛋白固有的免疫增强活性，还显著降低了其免疫原性；将其应用于含铝佐剂的猪圆环病毒2型亚单位疫苗时，可有效增强该疫苗的免疫原性，诱导体液和细胞免疫应答，并显著促进抗原特异性的Th1和Th2型免疫应答，对完善现有含铝佐剂亚单位疫苗的配方、提升疫苗免疫效果具有重要意义。

Tumor-targeting pharmaceutical combinations

Resumen de: WO2026150325A1

A pharmaceutical combination and uses thereof, wherein the pharmaceutical combination comprises a tumor-homing bacteria and a host-transient bacterial adjuvant comprising a supplementary dose of an immunostimulant.

Composition for improving intestinal injury related to salmonella infection and application thereof

Resumen de: CN122478915A

The invention belongs to life science, and particularly relates to a composition for improving intestinal injury related to salmonella infection and application of the composition. The invention aims to provide a composition for improving intestinal injury related to salmonella infection and application of the composition so as to solve the problem that in the prior art, active ingredient compositions and effective intervention schemes for infection related intestinal inflammation, mucous membrane injury and intestinal barrier function damage are lacked. The composition comprises an active ingredient related to tryptophan metabolism, and can be used for reducing inflammatory response, improving intestinal tissue damage and maintaining intestinal barrier function.

Traditional Chinese medicine composition for treating salmonella pullorum as well as preparation method and application of traditional Chinese medicine composition

Resumen de: CN122479009A

The invention discloses a traditional Chinese medicine composition for treating salmonella pullorum as well as a preparation method and application thereof, and belongs to the technical field of medical preparations. Screening is performed through network pharmacology, and the traditional Chinese medicine composition for treating salmonella pullorum is formed on the basis of the compatibility theory of monarch, minister, assistant and guide and the traditional Chinese veterinary pharmacy differentiation theory. The traditional Chinese medicine composition takes the root of Chinese pulsatilla and cortex phellodendri as monarch drugs, radix scutellariae and herba portulacae as ministerial drugs, agastache rugosus and rhizoma atractylodis macrocephalae as adjuvant drugs and liquorice as conductant drugs; the traditional Chinese medicine composition has the main effects of clearing away heat and toxic materials, eliminating dampness and stopping dysentery and the auxiliary effects of tonifying spleen and consolidating foundation, and has good treatment effect on salmonella pullorum

Medium-temperature sterilization process for lunch meat products and application of medium-temperature sterilization process

Resumen de: CN122478209A

The invention discloses a medium-temperature sterilization process for luncheon meat products and application of the medium-temperature sterilization process, enzymatic hydrolysate capable of inhibiting bacillus cereus, escherichia coli, staphylococcus aureus and salmonella is obtained by systematically optimizing a bullfrog skin enzymolysis process, freeze-dried powder of the enzymatic hydrolysate is added into luncheon meat raw materials according to the mass ratio of 0.4%, and medium-temperature sterilization treatment is combined, so that the luncheon meat products are obtained. Storage experiments prove that bacillus cereus, escherichia coli, staphylococcus aureus, shigella and salmonella in luncheon meat can be synergistically and efficiently inhibited, the total number of bacterial colonies is still stabilized to be smaller than 1.0 lg (CFU/g) after the luncheon meat is stored at 30 DEG C for 12 days, all pathogenic bacteria are negative, and the sensory quality and physicochemical indexes (TVB-N and TBARS) are remarkably superior to those of a traditional 121 DEG C high-temperature sterilization product. The safety and the quality of the luncheon meat are successfully and synergistically improved. A novel antibacterial peptide is identified from the enzymatic hydrolysate, the amino acid sequence of the novel antibacterial peptide is RGFPGLPGP, and the antibacterial peptide has a strong inhibition effect on bacillus cereus, escherichia coli, staphylococcus aureus and s

Microorganism recognition method

Resumen de: CN122484250A

Provided is a method for recognizing a microorganism in which a marker protein is selected and used, said marker protein having good reproducibility with respect to a strain of the genus Listeria and being capable of quickly recognizing the strain of the genus Listeria. A method for identifying microorganisms according to the present invention is characterized by comprising: a step for obtaining a mass spectrum by mass-analyzing a sample containing microorganisms; a reading step for reading the mass-to-charge ratio m/z of a peak derived from a labeled protein from the mass spectrum; and an identification step for identifying which strain of Listeria monocytogenes is contained in the microorganism contained in the sample on the basis of the mass-to-charge ratio m/z, using at least one of 17 ribosomal proteins L3, L4, L23, L2, L24, L6, L18, S5, L15, S13, S11, L10, L21, L13, S9, L31, and S16 as the labeled protein, and particularly using at least one of 8 ribosomal proteins L24, L6, L18, L15, S11, S9, L31, and S16 from among the 17 ribosomal proteins as the labeled protein.

Compound probiotic fermented feed capable of improving intestinal health of livestock and poultry and preparation method of compound probiotic fermented feed

Resumen de: CN122478137A

The invention discloses a compound probiotic fermented feed for improving intestinal health of livestock and poultry and a preparation method of the compound probiotic fermented feed, and relates to the technical field of livestock and poultry micro-ecological feeds. The composite probiotic fermented feed is composed of a fermentation substrate and a composite probiotic inoculant, and is prepared by the following steps: respectively carrying out amplification culture on each strain to the end of logarithm, mixing according to a ratio to obtain the composite inoculant, adjusting the moisture of the fermentation substrate to 38-47%, adjusting the pH value to 5.8-6.6, spraying the composite inoculant, fully and uniformly mixing, filling the mixture into a breathing bag or a one-way exhaust fermentation tank, and carrying out fermentation at the end of logarithm to obtain the composite probiotic fermented feed. Carrying out aerobic shallow fermentation for 1.5-3 hours at 28-37 DEG C, then carrying out closed anaerobic fermentation for 52-96 hours, air-cooling to room temperature through a low-temperature fluidized bed after fermentation is finished, carrying out gamma-ray low-dose infectious microbe inactivation, and then carrying out vacuum packaging. After livestock and poultry are fed with the feed, colonization of beneficial bacteria of cecum and rectum villus can be remarkably increased, abundance of escherichia coli and salmonella is reduced, chyme retention time is shorten

High-affinity antibody for resisting Shiga toxin type I

Resumen de: CN122483190A

The invention provides a high-affinity antibody for resisting shiga toxin I, belongs to the field of biological medicine, and particularly relates to a nano antibody PO4 capable of efficiently neutralizing shiga toxin I (Stroga toxin type 1, Stx-1), the antibody can specifically recognize the B subunit of the shiga toxin I, and the binding is dose-dependent. The compound shows a good protection effect in cell experiments, and has a function of resisting I-type shiga toxin poisoning.

Cerebrospinal fluid pathogen bedside instant detection chip and system based on flexible microfluidics and multi-mode nanoprobe

Resumen de: CN122487465A

The invention relates to the technical field of biomedical detection and flexible electronics, and discloses a bedside instant detection chip and system for cerebrospinal fluid pathogenic bacteria based on flexible microfluidics and a multi-mode nanoprobe. The detection chip comprises a flexible electrochemical sensing array and a multi-layer micro-fluidic device, the flexible electrochemical sensing array and the multi-layer micro-fluidic device are aligned and bonded under the assistance of oxygen plasma to form a disposable detection chip module, and the sensing array is modified with broad-spectrum capture molecules and is matched with an aptamer functionalized multi-mode nano-probe; the detection system is composed of the chip module and a portable dual-mode detection terminal. According to the method, efficient enrichment of pathogenic bacteria is realized through a broad-spectrum capture interface, electrochemical quantitative screening and Raman spectrum strain identification are completed by combining a multi-mode nanoprobe with dual-mode signal reading, and the problems of long detection period, poor specificity and difficulty in bedside operation in the prior art are solved.

Salmonella real-time serotyping method based on nanopore sequencing

Resumen de: CN122484316A

The invention discloses a salmonella real-time serotyping method based on nanopore sequencing, which comprises the following steps: step 1, in a sequencing process, reading newly generated read sequence data at regular time; step 2, comparing the read sequence data with a reference database; filtering according to comparison quality and comparison length threshold values, and retaining effective comparison results; 3, classifying effective comparison results according to sites; the first k optimal candidates are reserved for each read segment, weight distribution is normalized, and candidate reference sequence support degrees are accumulated; step 4, comparing the highest/second highest candidate support degree, and performing site judgment in combination with the coverage degree and the distinguishing index; and 5, continuously inputting new data, repeating the steps 1-4, and dynamically updating a result and a report. By utilizing the analysis method disclosed by the invention, the salmonella detection period can be obviously shortened; moreover, according to the method, the normalized weights of the read segments to the candidate reference sequences are accumulated through a soft allocation strategy, and the effective support degree is taken as a judgment basis, so that the accuracy and stability of an early judgment result can be improved.

Application of ABHD13 gene or protein in regulating innate immune response of poultry

Nº publicación: CN122479128A 31/07/2026

Solicitante:

INST OF ANIMAL SCIENCES CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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Resumen de: CN122479128A

The invention belongs to the field of gene engineering, and discloses application of an ABHD13 gene or protein in regulating and controlling innate immune response of poultry. The invention discloses the application of the ABHD13 gene or protein in regulating the innate immune pathway for the first time, clarifies the mechanism of regulating the innate immune pathway receptor linker protein NOD1 by the depalmitase ABHD13 from the angle, and provides a powerful scientific basis and tool for prevention, control and treatment of gram-negative bacteria such as salmonella and the like. The oligonucleotide of the target ABHD13 gene provided by the invention can significantly improve the phagocytic ability of chicken macrophages, and provides a scientific means for scientific research and industrial application related to prevention and control of gram-negative bacteria such as salmonella.