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LastUpdate Última actualización 14/03/2026 [07:13:00]
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Solicitudes publicadas en los últimos 90 días / Applications published in the last 90 days
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一种CeO2-Au复合材料结合TDN-MultiApt形成的MNP-DNA-Apt复合体及制备方法与应用

NºPublicación:  CN121142039A 16/12/2025
Solicitante: 
大连民族大学
CN_121142039_PA

Resumen de: CN121142039A

本发明公开了一种CeO2‑Au复合材料结合TDN‑MultiApt形成的MNP‑DNA‑Apt复合体及制备方法与应用,所述的复合体是通过下述方法制备得到的:先构建立方体c‑CeO2‑Au纳米颗粒,再构建DNA‑CeO2‑Au探针(DA探针),然后利用四个单链DNA样品构建TDN结构,并通过碱基互补配对与适配体Apt链接,形成TDN‑MultiApt;TDN‑MultiApt通过抗原‑抗体反应和碱基互补配对分别与链霉亲和素修饰的磁珠MNP和DA探针结合形成MNP‑DNA‑Apt复合体,即形成MDA探针。本发明中的立方体CeO2‑Au结合TDN‑MultiApt比色传感系统突破了传统的基于培养和其他分子诊断技术的局限性,该系统通过比色模式进行检测,适用于现场快速检测,突出了其作为现场检测解决方案的潜力。

Application of ramoplanin as antibacterial synergist of polymyxin antibiotics

Nº publicación: CN121130046A 16/12/2025

Solicitante:

SOUTH CHINA AGRICULTURAL UNIV
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CN_121130046_PA

Resumen de: CN121130046A

The invention belongs to the technical field of antibacterial synergists, and discloses application of ramoplanin as a colistin antibacterial synergist. The invention provides a treatment strategy for combined use of ramoplanin as a polymyxin drug sensitizer in treatment of gram-negative bacterial infection, and in a test of combined use of ramoplanin and colistin for detection of a bacterial sterilization curve and the integrity of a bacterial outer membrane, the concentration of the ramoplanin is low and is only 16 mu g/mL; in the aspect of the antibacterial effect, the FICI index is relatively low, and after the ramoplanin and the colistin are jointly used, the MIC (Minimum Inhibitory Concentration) of the colistin can be reduced by 16 times at most. The research shows that ramoplanin is a promising antibiotic sensitizer, and can be used in combination with the polymyxin drug colistin to resist the colistin drug resistance problem of escherichia coli, salmonella and klebsiella pneumoniae.

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