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Novel reference standard and improved method for quantification of saccharide in vaccine

Resumen de: GB2701058A

A method for quantification of O-specific polysaccharide (OSP) content in a sample, the method comprises: (a) adding a chromogen to the sample to obtain a mixture, where the sample is optionally diluted with a buffer prior to the addition of the chromogen; (b) mixing an acid to the mixture obtain a solution; (c) incubating the solution; (d) subjecting the incubated solution to a spectrophotometric assay or a colorimetric assay; and (e) quantifying the O-specific polysaccharide content in the sample using a mixed saccharide standard solution; where the mixed saccharide standard solution comprises at least two saccharides. The chromogen may be phenol, and the acid may be sulphuric acid. Preferably, the mixed saccharide standard solution comprises rhamnose, mannose, galactose, and paratose in a ratio of about 1:1:1:0.76:1. The method may be used in a method of quantifying the OSP of a conjugate vaccine formulation that induces an immune response against bacterial capsular polysaccharides, such as Typhoid Conjugate Vaccine - Salmonella paratyphi. no figure

INTRACELLULAR DELIVERY OF THERAPEUTIC CARGOS AND VIRAL RNAS BY ENGINEERED SALMONELLA

Nº publicación: EP4724111A2 15/04/2026

Solicitante:

UNIV COLUMBIA [US]

Resumen de: WO2024254419A2

Salmonella typhimurium comprising a first heterologous nucleic acid encoding a first polypeptide that causes bacterial lysis, a second heterologous nucleic acid encoding a second polypeptide that causes vacuolar lysis, and a third heterologous nucleic acid encoding a virus.