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Lactococcus lactis capable of producing nisin as well as construction and application of engineering bacteria of lactococcus lactis

Resumen de: CN120399983A

The invention relates to lactococcus lactis capable of producing nisin as well as construction and application of engineering bacteria of the lactococcus lactis. The invention provides a Lactococcus lactis ZLL111 strain, which is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No.31943. The Lactococcus lactis ZLL111 strain has the advantages that the Lactococcus lactis ZLL111 strain is prepared from Lactococcus lactis; the lactococcus lactis provided by the invention has a relatively strong antibacterial effect, produces nisin, has the characteristics of acid resistance, high temperature resistance, protease resistance and the like, and has a relatively strong anti-salmonella effect in vitro. Genetically engineered bacteria are constructed by using the bacteriocin, and the expression quantity is improved.

炭水化物ベースの抗サルモネラワクチンの開発

Resumen de: CN119907682A

Provided herein are vaccine compositions comprising a Salmonella antigen conjugated to a capsid wherein the capsid comprises a wild-type or native sequence. Also provided herein are vaccine compositions comprising a Salmonella antigen conjugated to a capsid wherein the capsid comprises at least one mutation, such as a non-natural mutation. Such compositions are useful in the prevention or treatment of Salmonella infection (salmonellosis), in the treatment and prevention of gastroenteritis, typhoid and/or paratyphoid; and the method can effectively aim at various salmonella strains.

COMPOSITIONS AND METHODS FOR SURFACE DISINFECTION

Resumen de: MX2025007790A

Methods and antimicrobial compositions are provided for surface cleaning. The antimicrobial compositions which include very low concentrations of an organic acid and one or more anionic surfactants can, unexpectedly, provide effective and broad spectrum antimicrobial activity within two minutes of contact with a surface and in some instances within one minute of contact time. Contacting the antimicrobial compositions with a surface can result in greater than or equal to about 4.0 log kill or 5.0 log kill for one or more of <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i>, and <i>Salmonella enterica </i>on the surface in two minutes or less. The compositions can have antimicrobial activity against at least one gram-positive bacterium, gram-negative bacterium, virus, and fungus in two minutes or less of contacting the composition with a surface.

Primer combination for multiple detection kit of food-borne pathogenic bacteria and multiple detection kit

Resumen de: CN120400381A

The invention provides a primer combination of a multiple detection kit for food-borne pathogenic bacteria and the multiple detection kit. The primer combination comprises probes and primers for detecting nine food-borne pathogenic bacteria: vibrio cholerae general type, vibrio cholerae O1 type, vibrio cholerae O139 type, vibrio parahaemolyticus, salmonella, yersinia enterocolitica, campylobacter, listeria monocytogenes and enterobacter sakakaakii, and the sequence is shown as SEQ1-31; the probe and the primer pair are used for detecting nine characteristic genes, namely a shigella universal type: ipaH, a diarrheogenic Escherichia coli universal type: eltA, sta3, sta and aggR, and enterohemorrhagic Escherichia coli: stx1, stx2, escV and rfbE, and the sequences of the probe and the primer pair are shown as SEQ32-64. According to the invention, by combining a digital PCR multiple coding technology, the detection of 12 food-borne pathogenic bacteria can be realized, and the sensitivity, the specificity and the accuracy are relatively good.

Lactobacillus farciminis SR2 and use thereof

Resumen de: GB2637451A

Disclosed are a lactobacillus farciminis SR2 and a use thereof. The present lactobacillus farciminis SR2 shows good tolerance under an acidic condition of pH = 3.0, has strong adaptability in 45 C° high-temperature and high salt (10% NaCl) environment, inhibits the growth of harmful bacteria such as escherichia coli, staphylococcus aureus, and salmonella, has high productivity of ferulic acid esterase, can significantly lower the pH level of rice straw silage feed, significantly increase the content of lactic acid, significantly reduce the content of ammonia nitrogen, significantly increase the content of dry matter and WSC, significantly reduce the content of NDF, ADF and cellulose, improve the fermentation quality of the rice straw silage feed, and the method can be widely applied to the field of rice straw fermentation feed preparation.

SALMONELLA STRAIN WITH CHROMOSOMALLY INTEGRATED LANDING PAD

Resumen de: CN119923470A

The present invention relates to a modified live attenuated Salmonella strain comprising at least one chromosomal integrated synthetic polynucleotide sequence inserted in a predetermined pseudogenomic position, said sequence comprising at least one defined recombination site for introducing a heterologous polynucleotide sequence encoding a polypeptide, wherein the chromosome integration synthetic polynucleotide sequence is located within at least one genomic site, the genomic site being defined by any one of SEQ ID NO: 1-30 or a sequence having at least 70% identity with any one of SEQ ID NO: 1-30. The invention also relates to vaccine compositions comprising the modified strains and various uses and methods thereof.

Application of combination of ebbeselenium and polymyxin in preparation of salmonella infection resisting medicine

Resumen de: CN120381506A

The invention belongs to the technical field of biological medicine, and discloses application of ebb-selenium as a polymyxin synergist in inhibition of salmonella. According to the application, the combination of ebb selenium and polymyxin has a good synergistic effect on salmonella in vitro and in vivo experiments. In a mouse salmonella infection model, compared with the bacterium carrying amount after single-drug treatment, the bacterium carrying amount in the animal liver after EBS and polymyxin combined treatment has the effect that the bacterium carrying amount in the animal liver is obviously reduced. The survival rate of animals treated by the combination of the EBS and the polymyxin is obviously increased compared with the survival rate of the animals treated by single medicines of the EBS and the polymyxin. Compared with the traditional method for killing salmonella through combination of antibiotics, the method has the advantages that the generation of bacterial drug resistance is not easily induced through the synergistic sterilization of the ebb-selenium and the polymyxin, and the ebb-selenium has the characteristics of wide source, multiple effects and good treatment effect, and has better research and application significance for excavating the synergistic replacement of the antibiotics and solving the bacterial drug resistance.

Method and kit for rapidly testing sensitivity of salmonella to ceftriaxone

Resumen de: CN120384115A

The invention discloses a method for rapidly testing sensitivity of salmonella to ceftriaxone. The method comprises the following steps: S1, preparing a salmonella solution; s2, centrifuging the bacterial liquid and removing supernate of the centrifuged bacterial liquid; s3, providing a ceftriaxone solution, wherein the ceftriaxone solution contains ceftriaxone and water; s4, mixing the ceftriaxone solution with the bacterial solution to form a mixed solution, placing the mixed solution in an incubator, and standing for a first preset time; s5, centrifuging the mixed solution after standing for a first preset time, and extracting supernate of the mixed solution; and S6, identifying the ceftriaxone in the supernatant of the mixed solution by using a time-flight mass spectrometer, and judging the sensitivity of the salmonella to the ceftriaxone according to whether a characteristic peak corresponding to the ceftriaxone disappears or not. Compared with PCR (polymerase chain reaction) and LAMP (loop-mediated isothermal amplification) methods, the method has the advantages that the detection result is more reliable, the time consumed by the method is less than that consumed by manual drug sensitivity and full-automatic drug sensitivity identification instruments, and the method can be used for conventional detection in laboratories to timely feed back drug sensitivity results to clinics.

Broad-spectrum salmonella bacteriophage SD40-16 and application thereof

Resumen de: CN120381470A

The invention belongs to the technical field of microbial sterilization preparations, and particularly discloses a broad-spectrum salmonella bacteriophage SD40-16 and application of the broad-spectrum salmonella bacteriophage SD40-16. The bacteriophage SD40-16 can be used for cracking six serotypes of salmonella and escherichia coli including salmonella enteritidis and salmonella typhimurium, is wide in host range and strong in killing capability, and can be used for inhibiting the growth of bacteria. And meanwhile, the strain has good thermal stability and acid-base tolerance, and is easy to proliferate and enrich. The bacteriophage can enhance the antibacterial ability of kanamycin, provides a new drug combination strategy, has an obvious synergistic effect on salmonella resistance when being combined with kanamycin, and has a good application prospect in the aspect of preventing and treating salmonella infection.

Pharmaceutical composition or vaccine for treating and/or inhibiting salmonella infection and application of small molecule compound Epetrabole hydrochloride

Resumen de: CN120361018A

The invention discloses a pharmaceutical composition or a vaccine for treating and/or inhibiting salmonella infection, and an application of a small molecule compound Epetrabole hydrochloride. The invention further discloses an application of the pharmaceutical composition or the vaccine and the small molecule compound Epetrabole hydrochloride for treating and/or inhibiting salmonella infection. The MIC value of the small molecule compound is 0.48 mu M, and compared with existing antibiotics for clinically treating salmonella enteritidis infection, the small molecule compound has relatively strong antibacterial activity when the small molecule compound is in a trace amount. An in-vitro killing bacteriostatic activity experiment result shows that the small molecule compound has a certain effect on reduction of the number of bacteria. The Salmonella enteritidis strain has obvious antibacterial activity on a J774A.1 cell model, and can inhibit the expression of a salmonella enteritidis virulence factor T3SS1 in a targeted manner. According to the present invention, the small molecule compound Epetrabole hydrochloride provides a certain inhibition effect on salmonella isolates from different sources, and has a wide application value;

Compound preparation for preventing and treating bacterial diseases of livestock and poultry and preparation method thereof

Resumen de: CN120361208A

The invention discloses a compound preparation for preventing and treating bacterial diseases of livestock and poultry and a preparation method of the compound preparation. The compound preparation is prepared from the following components in parts by weight: 3.2 to 5.8 parts of astragalus polysaccharide powder, 2.9 to 5.3 parts of xylooligosaccharide powder, 2.5 to 4.8 parts of compound enzyme agent, 2 to 4.4 parts of compound bacterium agent, 1.5 to 3.6 parts of selenium yeast powder, 1.3 to 3.2 parts of egg yolk antibody, 1 to 2.1 parts of hydroxypropyl methyl cellulose, 0.35 to 0.8 part of chitosan and 0.3 to 0.65 part of montmorillonite. The compound preparation disclosed by the invention can effectively inhibit growth and reproduction of viruses such as riemerella anatipestifer, escherichia coli and salmonella, and is beneficial to regulation of immune functions of animals and improvement of body resistance of the animals.

一种四基因敲除减毒鼠伤寒沙门氏菌及其构建方法和应用

Resumen de: CN120366177A

本发明提供一种四基因敲除减毒鼠伤寒沙门氏菌及其构建方法和应用，所提供的一种四基因敲除减毒鼠伤寒沙门氏菌，是敲除了鼠伤寒沙门氏菌的relA、Asd、manA和sifA基因。本发明构建了四种毒力因子缺失的鼠伤寒沙门氏菌的减毒菌株ΔrelA‑ΔAsd‑ΔmanA‑ΔsifA SM14028，可在体外稳定传代。并经安全性评价试验检测，该菌株毒力无毒力，安全性极高。为了进一步评价ΔrelA‑ΔAsd‑ΔmanA‑ΔsifA SM14028是否能够抵抗亲本株的攻击，进行了免疫效力评价，结果显示，ΔrelA‑ΔAsd‑ΔmanA‑ΔsifA SM14028能够提供有效的免疫保护。因此，本发明的ΔrelA‑ΔAsd‑ΔmanA‑ΔsifA SM14028可作为一种有效的活疫苗或者活载体疫苗，为动物疫病的防控奠定基础。

抗原タンパク質または該タンパク質をコードする遺伝子を有する細胞外小胞およびその用途

Resumen de: CN119630803A

The present invention relates to an extracellular vesicle containing an antigen protein or a gene encoding the protein, and a use thereof, and more specifically, to an extracellular vesicle containing an antigen protein derived from a virus, a microorganism or a cancer cell or a gene encoding the protein, or a vaccine composition for preventing or treating viral infections, microbial infections or cancers comprising the same. The extracellular vesicle or the vaccine composition comprising the same according to the present invention is a platform applicable to various diseases, has excellent antigen-specific immune response induction effect and stability, and is expected to be effectively used in the field of vaccine development. The vaccines are useful for the prevention or treatment of various diseases including viral infections, microbial infections or cancers.

METHOD FOR TESTING IN-VITRO BACTERICIDAL FUNCTION OF TYPHOID VACCINE IMMUNE SERUM

Resumen de: WO2025152055A1

Provided is a method for testing the in-vitro bactericidal function of a typhoid vaccine immune serum, comprising: testing the in-vitro bactericidal function of a typhoid vaccine immune serum against Salmonella typhi bacteria by means of an opsonophagocytic killing assay, wherein when the phagocytic killing step of the opsonophagocytic killing assay is completed, the Salmonella typhi bacteria having undergone the phagocytic killing step are cultured at the pH of 7.5-9.5. The opsonophagocytic killing assay is applied to Gram-negative Salmonella Typhi bacteria for the first time, and the in-vitro bactericidal levels of typhoid vaccine immune serums are effectively and accurately evaluated.

Lactobacillus plantarum with broad-spectrum antibacterial function

Resumen de: CN120349938A

The invention discloses a broad-spectrum antibacterial lactobacillus plantarum, and belongs to the technical field of microorganisms. The lactobacillus plantarum 9-6 is separated from animal feed, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32568, the lactobacillus plantarum 9-6 and fermentation liquor thereof have an obvious inhibition effect on common vibrio parahaemolyticus, vibrio alginolyticus, vibrio harveyi and vibrio vulnificus, and the lactobacillus plantarum 9-6 and the fermentation liquor thereof have the advantages that the lactobacillus plantarum 9-6 and the fermentation liquor thereof have the obvious inhibition effect; and in addition, the antibacterial agent has a relatively good antibacterial effect on common staphylococcus aureus, escherichia coli, listeria monocytogenes, salmonella enterica and bacillus cereus. The lactobacillus plantarum 9-6 has probiotic safety, can grow under an initial pH condition of 4-10 and a high-salt environment, and plays a role in bacteriostasis under an acidic condition. The invention lays a foundation for developing a good antibacterial probiotic preparation and application of a fermentation culture thereof.

Poultry salmonella drug resistance detection equipment

Resumen de: CN120349878A

The poultry salmonella drug resistance detection equipment comprises a bottom plate, the upper end of the bottom plate is fixedly connected with a first vertical plate and a second vertical plate which are arranged left and right, the upper end of the bottom plate is provided with a positioning frame located between the first vertical plate and the second vertical plate, and the upper end of the positioning frame is provided with a storage mechanism; a discharging opening located in the inner side of the positioning frame is formed in the bottom plate in a penetrating mode, a bearing mechanism is arranged on the inner side of the discharging opening, a positioning ring located on the left side of the positioning frame is arranged at the upper end of the bottom plate, a guide rod is fixedly connected between the first vertical plate and the second vertical plate, and the guide rod is sleeved with a sliding sleeve in a sliding mode. According to the device, continuous operation of taking and moving the drug sensitive paper and pasting the drug sensitive paper into the culture dish can be completed, mechanical linkage is utilized, reliability is good, independent driving is not needed, cost is reduced, the situation that the drug sensitive paper is manually taken out of the containing plates in sequence and then taken for pasting operation is not needed, the experiment efficiency is improved, and meanwhile the pollution risk is reduced.

Method for rapidly detecting salmonella pullorum by using fluorescent quantitative PCR (Polymerase Chain Reaction) and primer used by method

Nº publicación: CN120350143A 22/07/2025

Solicitante:

JIANGSU POLYTECHNIC COLLEGE OF AGRICULTURE AND FORESTRY
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Resumen de: CN120350143A

The invention discloses a method for rapidly detecting salmonella pullorum by fluorescent quantitative PCR (polymerase chain reaction) and used primers, and the method for rapidly detecting salmonella pullorum by fluorescent quantitative PCR specifically comprises the following steps: (1) collecting a sample; (2) nucleic acid extraction; (3) preparing a reaction system; (4) running a sample detection program; and (5) detecting a test result and judging. According to the rapid detection method disclosed by the invention, an ultra-short-range PCR program is designed as follows: pre-denaturation is performed at 95 DEG C for 2 minutes; according to the present invention, with the program setting, the salmonella pullorum detection time is shortened by about 60% compared with the salmonella pullorum detection time of the commercially available kit and the commercial standard program, and the detection efficiency is substantially improved; the cost of a single detection reagent is reduced by 40% compared with that of an imported kit, and the economic cost is reduced; compared with a traditional culture method, the method has the advantages that the time is 3-5 days earlier, the detection result is reliable, the repeatability is good, and the method can be used for clinical diagnosis and monitoring of SP.