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Probiotic product for inhibiting salmonella in chicken body and screening method

Resumen de: CN120888460A

The invention relates to the technical field of microorganisms, in particular to a probiotic product for inhibiting salmonella in a chicken body and a screening method, the probiotic product comprises Chencity bacillus sachariensis, and the colony count of the Chencity bacillus sachariensis in the probiotic product is 107-1010 CFU/mL. Based on the screening method of the probiotics for inhibiting the salmonella in the chicken body, the screened city bacillus sachariensis is prepared into the probiotic product for inhibiting the salmonella in the chicken body, so that the salmonella infection condition of the chicken can be effectively improved, the weight of the chicken with positive salmonella infection is increased, and the salmonella in the chicken body is inhibited. The salmonella loading quantity in chicken bodies is obviously reduced, the salmonella loading quantity has the characteristics of low residual quantity and environment friendliness, and the problems of antibiotic residues, bacterial drug resistance increase and the like caused by antibiotic abuse can be avoided. By adopting the screening method of the probiotics for inhibiting the salmonella in the chicken body, provided by the invention, the dominant probiotics capable of inhibiting the salmonella can be simply, conveniently and efficiently determined.

PCR (Polymerase Chain Reaction) detection kit for rapidly identifying salmonella gallinarum with pullorum disease

Resumen de: CN120888393A

The invention relates to the technical field of pullorum disease, in particular to a PCR (Polymerase Chain Reaction) detection kit for quickly identifying salmonella gallinarum with pullorum disease, which comprises a detection system and a shell, and a top cover is hinged to one side of the top of the shell; a loading plate is vertically arranged in the shell in a sliding fit manner, a plurality of loading ports for loading PCR tubes are formed in the loading plate, and loading bags are fixedly connected to the loading ports; an oscillating assembly used for oscillating the loading bag and a limiting assembly used for limiting the position of the loading plate are arranged in the shell. The oscillation assembly comprises an oscillation plate, a controller and a driving part, and the controller is used for controlling the driving part to operate; the oscillating plate is located below the loading bag and is in vertical sliding fit with the inner side wall of the shell; the driving piece is fixedly connected to the inner bottom wall of the shell, and an output shaft of the driving piece is fixedly connected with a stirring wheel. Through the synergistic effect, the oscillation plate can achieve the efficient oscillation effect, it is ensured that reactants are evenly distributed in a PCR tube, and the uniformity and efficiency of the PCR reaction are improved.

Salmonella typhimurium sensor for regulating CRISPR/Cas12a activity based on DNAzyme conformation conversion and application

Resumen de: CN120888680A

The invention provides a salmonella typhimurium sensor for regulating and controlling CRISPR (clustered regularly interspaced short palindromic repeats)/Cas12a (CRISPR/Cas12a) activity on the basis of DNAzyme conformation conversion and The biosensor comprises an Apt-T composite probe, a hairpin D chain, a hairpin TS chain, crRNA, Cas12a, Mg < 2 + > and FQ. The Apt-T composite probe is obtained by hybridizing Apt and a T chain; and the 44th site of the TS chain is ribonucleotide. The biosensor provided by the invention is low in detection limit, and high-specificity detection of a target object is realized by virtue of specific recognition of the nucleic acid aptamer and combination of the aptamer and salmonella typhimurium. The detection limit of the biosensor is superior to that of most existing methods; and the background signal of the detection sample is also reduced. The sensor has the advantages of high detection speed, simplicity in operation, low detection limit, high specificity and the like, can make up the defects and deficiencies of the existing salmonella typhimurium detection, and realizes rapid and accurate quantitative detection of salmonella typhimurium.

Rutin and fangchinoline synergistic compound preparation for resisting salmonella infection and application

Resumen de: CN120884601A

The invention relates to a compound preparation for treating salmonella infection, which comprises rutin and fangchinoline, and the concentration ratio of the fangchinoline to the rutin is 1: 2. The compound preparation plays an antibacterial role through an anti-virulence strategy and a host-oriented strategy, remarkably reduces the bacteria loading amount of salmonella in tissue organs and intestinal contents of mouse and chick models, improves tissue pathological damage, and has good safety. The compound preparation disclosed by the invention provides an efficient and safe novel scheme for treating salmonella infection.

ANTI-BACTERIAL PROTEIN COMPLEX

Resumen de: MX2025009214A

The invention relates to protein bacteriocins (PBs) as therapeutic agents, and specifically to protein complexes comprising two or more PB molecules associated with a protein scaffold which comprises cognate immunity protein domains for the effector portions of the respective PBs. In particular, the invention provides an anti-bacterial protein complex comprising (a) a first PB molecule and a second PB molecule; and (b) an immunity protein scaffold comprising a first immunity protein domain and a second immunity protein domain; wherein the first and second immunity protein domains are non-covalently bound to the respective first and second PB molecules.

PD-L1 Salmonella mutant expressing anti-PD-L1 peptide and outer membrane vesicle thereof and pharmaceutical comprising the same

Resumen de: KR20230091594A

The present invention relates to a Salmonella mutant strain expressing anti-PD-L1 peptide, outer membrane vesicles thereof, and a pharmaceutical composition containing the same. The Salmonella mutant strain expresses the anti-PD-L1 peptide in an extracellular loop of an outer membrane protein A, and thus, the anti-PD-L1 peptide can be delivered stably and efficiently to the inside of the tumor due to the characteristics of Salmonella, and can exhibit an anti-tumor effect by inducing the activation of T cells through the formation of a bond with PD-1 of cancer cells, thereby using the Salmonella mutant strain or the membrane vesicles thereof for cancer treatment.

Goose source Weissella cibaria F11 and application thereof

Resumen de: CN120866142A

The invention relates to the technical field of probiotic development and utilization, in particular to a goose source Weissella cibaria F11 and application thereof. The Weissella cibarius F11 is preserved in the China General Microbiological Culture Collection Center on July 11, 2025, and the preservation number is CGMCC NO.35211. The Weissella cibarius F11 has the characteristics of good acid resistance and cholate resistance, and can be used for preparing the acid-resistant and cholate-resistant strain. And the bacillus subtilis has a good inhibition effect on pathogenic bacteria such as escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and salmonella typhimurium, and also has stable acid production capacity and good safety. The Weissella sinus F11 provided by the invention meets the probiotic characteristic standard, the antibiotic resistance spectrum of the Weissella sinus F11 meets the feed probiotic safety standard, the growth of goslings can be remarkably promoted, the death rate and diarrhea occurrence rate of the goslings can be remarkably reduced, and the weissella sinus F11 can be used for preparing feed probiotics. The development potential in the fields of preparation of bacteriostatic and antibacterial drugs, probiotics for poultry feed, feed additives, poultry feed and the like is huge.

Application of combination of denosine and polymyxin in preparation of antibacterial drugs

Resumen de: CN120860179A

The invention belongs to the technical field of medicines, and particularly relates to application of pinosine and polymyxin in preparation of antibacterial drugs, and the antibacterial drugs aim at one or more of escherichia coli, salmonella and klebsiella pneumoniae. The antibacterial agent aims at bacteria which are resistant to polymyxin. The bacteria directed by the antibacterial agent are multi-drug-resistant gram-negative bacteria. A checkerboard method minimum inhibitory concentration test and an in-vitro time sterilization curve prove that the pinosine effectively recovers the sensitivity of bacteria to the polymyxin, and meanwhile, a staining experiment and fluorescent quantitative PCR prove that the pinosine can effectively recover the sensitivity of the bacteria to the polymyxin by destroying the completeness of a bacterial cell membrane and inhibiting the transcription expression of a polymyxin drug-resistant gene mcr-1. The antibacterial activity of the polymyxin on bacteria is effectively enhanced. The invention provides a novel application of densipine in enhancing the antibacterial activity of polymyxin antibiotics, and can solve the technical problems of low clinical drug resistance, low therapeutic index and the like of polymyxin.

Salmonella typhimurium aptamer lateral flow chromatography test strip based on photothermal effect of ferroferric oxide and colloidal gold composite nanomaterial as well as preparation method and application of salmonella typhimurium aptamer lateral flow chromatography test strip

Resumen de: CN120870552A

The invention discloses a salmonella typhimurium aptamer lateral flow chromatography test strip based on the photothermal effect of a ferroferric oxide and colloidal gold composite nanomaterial, a preparation method and application, and belongs to the technical field of lateral flow chromatography test strips. The preparation of the probe comprises the step of preparing a Fe3O4 (at) Au composite nano material; the preparation method comprises the following steps: under the protection of nitrogen, dissolving FeCl3. 6H2O and FeCl2. 4H2O in deionized water, adding ammonia water, stirring at room temperature, adding trisodium citrate, collecting by using a magnet, then adding into a boiling HAuCl4 solution, keeping boiling until the color of the solution becomes reddish brown, continuing heating, cooling, and collecting by using a magnet; the test strip provided by the invention can realize quantitative detection of salmonella typhimurium.

Probe, kit and detection method for detecting salmonella

Resumen de: CN120866303A

The invention provides a probe, a kit and a detection method for detecting salmonella, and belongs to the technical field of pathogenic bacterium detection. The research provides an unmarked fluorescent biosensing platform for high-sensitivity detection of salmonella. A target sequence is introduced into a catalytic core sequence of Aurora DNAzyme, so that a signal probe E-Aurora is constructed. The E-Aurora can be combined with a substrate 4-methylumbelliferone phosphate disodium salt to generate a strong fluorescent compound 4-MU. When salmonella exists, PfAgo is activated and specifically cuts the E-Aurora probe, so that the structural integrity of E-Aurora is destroyed and the catalytic ability of E-Aurora is lost, and the generation of a fluorescence signal is hindered. The biosensor realizes ultra-high sensitivity detection of salmonella, the limit of detection (LOD) is as low as 1 CFU/mL, and an ideal recovery rate (80-120%) is obtained in actual sample detection. The detection method disclosed by the invention is simple to operate and wide in application range, and can be used for determining trace salmonella in food.

SYSTEMS, METHODS, AND COMPOSITIONS FOR THE DETECTION OF MICROBES

Resumen de: AU2024264505A1

Provided are non-naturally occurring systems, methods, and compositions for the detection of microbes. The disclosure relates to the detection of an antigen specific to a microbe, such as a foodborne, an environment-borne, and a bloodborne bacteria, using capture antibody and moiety, detector antibody and moiety, and a light-emitting particle.

HOST CELL PROTEIN DETECTION USING LATERAL FLOW DEVICES

Resumen de: US2025334572A1

Time sensitive biopharmaceutical processing updates can be implemented using lateral flow devices to test on-site during manufacturing. Lateral flow testing for an analyte such as, for example, impurities (e.g., host cell proteins), during manufacturing of a biopharmaceutical drug product is completed within twenty-five minutes of collecting a sample from the manufacturing line. That is, all sample preparation, such as adding reagent or diluting the sample, contacting the prepared sample to a lateral flow device, and detecting presence or absence of the analyte is completed within twenty-five minutes (e.g., 20 minutes, 18 minutes, 15 minutes) from collection such that processing adjustments during manufacturing can be implemented.

METHOD FOR DETECTING FOODBORNE PATHOGENS USING BACTERIOPHAGES

Resumen de: WO2025225874A1

The present invention relates to a method for detecting live foodborne pathogens using novel bacteriophages LEC1, LBC9, and LSE2. By using this method, Escherichia coli, Bacillus cereus, and Salmonella spp., which are live foodborne pathogens in food, can be rapidly and accurately detected at the same time, and thus the method can be effectively used for preventing food poisoning.

VACUOLE ESCAPE

Resumen de: WO2025224268A1

The present invention relates to a live attenuated Gram-negative bacterium modified to enable enhanced vacuole escape. In particular, the invention relates to a live attenuated Gram-negative bacterium comprising a heterologous polynucleotide encoding a prokaryotic disulfide bond isomerase, or functional fragment thereof, wherein said heterologous polynucleotide encoding the prokaryotic disulfide bond isomerase is operably linked to a promoter, or a live attenuated Gram-negative bacterium comprising an endogenous prokaryotic disulfide bond isomerase, or functional fragment thereof, wherein the endogenous prokaryotic disulfide bond isomerase is upregulated compared to its basal level expression.

MODIFIED MICROORGANISMS

Resumen de: WO2025224251A1

The present invention relates to a live attenuated Gram-negative bacterium comprising a modified hlyCABD operon, wherein the modified hlyCABD operon is split into a first segment and a second segment, the first segment being operably linked to a first independently controlled promoter, wherein the first independently controlled promoter is a strong constitutive promoter or a strong vacuole-induced promoter, and the second segment being operably linked to a second independently controlled promoter, wherein the second independently controlled promoter is a strong vacuole-induced promoter, and wherein the first segment comprises a heterologous polynucleotide encoding a lysin upstream of a hlyAs translocation sequence, wherein the heterologous polynucleotide encoding one or more cargo molecules replaces a hlyA gene, and wherein the second segment comprises hly genes involved in secretion.

Composition or kit for detecting animal intestinal microorganism

Resumen de: KR20250155130A

본 발명은 서울특별시 서울기술연구원 2022년도 캠퍼스타운 기술매칭 지원사업(반려동물 장내미생물 자가검사키트)을 통해 개발된 기술이다. 본 발명은 동물의 장내미생물 검출용 조성물 또는 키트에 관한 것으로서, 본 발명의 조성물 또는 키트는 미생물 유래 독소에 특이적으로 결합할 수 있는 물질을 프로브로 포함함으로써, 시중에 널리 사용되는 코로나 자가검진 키트와 마찬가지로 소비자 스스로 반려동물의 장내미생물을 검사할 수 있도록 하고, 이에 따라 반려동물의 장내미생물 분석에 대한 소비자 접근성을 크게 향상시킬 수 있다.

HOST CELL PROTEIN DETECTION USING LATERAL FLOW DEVICES

Resumen de: WO2025224621A1

Time sensitive biopharmaceutical processing updates can be implemented using lateral flow devices to test on-site during manufacturing. Lateral flow testing for an analyte such as, for example, impurities (e.g., host cell proteins), during manufacturing of a biopharmaceutical drug product is completed within twenty-five minutes of collecting a sample from the manufacturing line. That is, all sample preparation, such as adding reagent or diluting the sample, contacting the prepared sample to a lateral flow device, and detecting presence or absence of the analyte is completed within twenty-five minutes (e.g., 20 minutes, 18 minutes, 15 minutes) from collection such that processing adjustments during manufacturing can be implemented.

BACTERIOPHAGES AND USE THEREOF FOR THE TREATMENT OR PREVENTION OF INFECTION CAUSED BY SALMONELLA GALLINARUM

Resumen de: WO2024137831A2

A composition of bacteriophages comprising at least one phage selected from TTS1, TTS2, TTS3, TTS4, TTS5, and TTS6, wherein the phages are optionally encapsulated; and a method for preventing or treating an infection caused by Salmonella Gallinarum by administering to a subject a therapeutically effective amount of the composition.

- Recombinant Microbials Expressing Strep-tag and Tumor Imaging Aduvant Comprising the Microbials

Resumen de: KR20240012662A

The present invention relates to a genetic construct characterized in that the anti-sigma factor flgM gene and the gene encoding strep-tag are linked to encode a fusion protein of flgM and strep-tag so that a strep-tag may be expressed on the surface of an anticancer strain that may be targeted to a tumor site and stimulate immune activity, and an anticancer adjuvant and a tumor imaging aid which is transformed thereby and exhibits anticancer activity itself and may be usefully used for the diagnosis and treatment of tumor cells together with an anticancer substance or contrast agent labeled with a substance which specifically reacts with the strep-tag.

METHODS AND SYSTEMS FOR THE RAPID DETECTION OF LISTERIA USING INFECTIOUS AGENTS

Nº publicación: EP4641200A2 29/10/2025

Solicitante:

LABORATORY CORP AMERICA HOLDINGS [US]
Laboratory Corporation of America Holdings

Resumen de: EP4641200A2

Disclosed herein are methods and systems for rapid detection of microorganisms such as Listeria spp. in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Listeria-specific bacteriophage, allows detection of a specific microorganism, such as Listeria spp. and an indicator signal may be amplified to optimize assay sensitivity.