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Resultados 1000 a 498 de 498
Resumen de: WO2024137831A2
A composition of bacteriophages comprising at least one phage selected from TTS1, TTS2, TTS3, TTS4, TTS5, and TTS6, wherein the phages are optionally encapsulated; and a method for preventing or treating an infection caused by Salmonella Gallinarum by administering to a subject a therapeutically effective amount of the composition.
Resumen de: EP4641200A2
Disclosed herein are methods and systems for rapid detection of microorganisms such as Listeria spp. in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Listeria-specific bacteriophage, allows detection of a specific microorganism, such as Listeria spp. and an indicator signal may be amplified to optimize assay sensitivity.
Resumen de: CN120842432A
本发明涉及一种应对多种致病性肠杆菌感染防治的融合蛋白、免疫原性组合物、重组简并疫苗，以及分子架构设计和应用等。本发明筛选到3种细胞免疫抗原Tuf、DnaK、fusA，构建的3种抗原的融合蛋白分子可显著抑制多种肠杆菌感染引起的组织病变，具有良好的免疫原性，起到有效防治和免疫保护作用，广谱且高效地预防多种致病性肠杆菌感染，具有广阔的工业应用前景。
Resumen de: WO2024192346A1
Technologies for providing immunogenic compositions (e.g., vaccines) and methods of inducing immune responses in subjects in need thereof.
Resumen de: US2025321205A1
Methods and devices are disclosed for rapid, multiplex molecular detection of diverse nucleic acid target molecules. The invention features an electrophoretic array with immobilized hydrogel microgel deposits. Each deposit comprises a three-dimensional, cross-linked polymer matrix containing an immobilized affinity-binding molecule and a porogen-derived pore network. This structure is configured for rapid molecular transport of nucleic acids (e.g., up to 800 bp), providing a localized environment for target capture, ligation of linear Rolling Circle Amplification (RCA) probes, and RCA. Target-specific components are anchored within distinct microgels for multiplexing. Electric fields enhance transport, reaction kinetics, and amplicon concentration. Detection is achieved in under 20 minutes. The specifically structured and fabricated microgels improve detection speed, sensitivity, and applicability to multiple different targets.
Resumen de: US2025321227A1
The present invention relates to diagnostic platform. More specifically, the invention relates to a dual platform composed of a detection and quantification unit and a characterization unit, systems, kits, methods and uses thereof in detection, quantification and characterization of pathogens, the system comprising monoclonal-antibody-based biosensor chips, for detection and/or quantification of pathogens in a sample, and substrate-based biosensor chips for enzymatically profiling the pathogen in the sample.
Resumen de: CN120757621A
本发明公开了一种提高重组蛋白热稳定性和溶解度的双功能蛋白标签及基于其的产品和应用，属于蛋白工程技术领域。该双功能标签的氨基酸序列SEQ ID NO:1所示，具有70%‑95%以上的相似度，其氨基酸序列变异主要存在但不限于二级结构位置。该双功能标签可在目的蛋白的N端或C端使用，适用于原核表达系统中融合蛋白的表达和纯化。本发明通过理性设计改造天然蛋白的疏水性区域，解决了现有蛋白标签分子量大、易遮蔽目标蛋白活性结构域、标签切除后残留非天然氨基酸可能破坏蛋白正确折叠等技术问题，显著提升融合蛋白在大肠杆菌表达系统中的可溶性及热稳定性，适用于包涵体蛋白的功能性表达与工业化应用。
Resumen de: JP2025149945A
【課題】 本発明が解決しようとする課題は、サルモネラの検出を、血清型横断的かつ他の菌種とは特異的に行うことである。【解決手段】サルモネラの加熱死菌体とアジュバントを含む抗原液を、ＢＡＬＢ／ｃマウスに接種した。接種後、マウスの脾細胞またはリンパ節細胞とＰ３Ｕ１とを融合させてハイブリドーマを作製し、その中から、サルモネラ特異性を示すモノクローナルＩｇＧ抗体を産生するハイブリドーマを選抜し、優れたサルモネラ特異性抗体を産生する３つのハイブリドーマを取得することに成功した。次いで、これらのモノクローナル抗体を用いて、サンドイッチＥＬＩＳＡにより複数血清型のサルモネラおよび他の菌種の検出を行ったところ、血清型横断的にサルモネラを検出し、他の菌種を検出しないという結果を得た。【選択図】図１
Resumen de: CN120737217A
本发明提供了一种亚单位疫苗及其制备方法与应用。所述疫苗包括式（1）的融合蛋白，D0a‑D1a‑Linker‑HA‑Linker‑D1b‑D0b（1）其中，D0a和D0b分别为细菌鞭毛蛋白FliC的D0结构域或其功能性片段的N端部分和C端部分，D0a和D0b组成细菌鞭毛蛋白FliC的D0结构域或其功能性片段；D1a和D1b分别为细菌鞭毛蛋白FliC的D1结构域或其功能性片段的N端部分和C端部分，D1a和D1b组成细菌鞭毛蛋白FliC的D1结构域或其功能性片段；HA为流感病毒HA蛋白的抗原表位或其功能性片段，linker为连接子，‑为肽键或其他化学键。可以用于流感病毒的净化工作，安全有效，具有较高的应用价值。
Resumen de: US2025304662A1
Among the various aspects of the present disclosure is the provision of FimH inhibiting compositions and methods of use thereof. FimH inhibiting compositions that target and inhibit FimH, including monoclonal antibodies, are described. Methods of identifying FimH inhibiting antibodies are also described. Further, a method of treating bacterial infections, including urinary tract infections, is described.
Resumen de: WO2025202803A1
The process comprises providing a recombinant vaccine construct, wherein said construct comprises genetically modified Lactobacillus plantarum NC8 as a live vector; modifying the genetic structure of said Lactobacillus plantarum NC8 to express conserved Salmonella antigens, including PagN, SopE2, and FliC, anchored by a ptrk 892 backbone with a constitutive promoter, phosphoglycerate mutase (PGM); incorporating Signal Lp_2145 and cAM12 Anchor sequences into said genetic construct to enhance surface expression of recombinant proteins on Lactobacillus plantarum NC8; administering said recombinant vaccine orally to poultry, leveraging the probiotic properties of Lactobacillus plantarum NC8 for effective colonization of the poultry gastrointestinal tract; inducing a prolonged and intensified immune response by ensuring sustained high-level expression of target antigens through the utilization of the robust constitutive promoter, phosphoglycerate mutase (PGM); and optimizing immunogenicity through the surface expression of recombinant proteins on Lactobacillus plantarum NC8, fostering a robust and precisely targeted immune response.
Resumen de: EP4623922A2
The invention relates to guided nucleases, CRISPR/Cas systems, crRNAs, single gRNAs, vectors, methods and pharmaceutical compositions, for example for targeting sporulating bacteria, or for targeting C difficile, Salmonella, E coli or Streptococcus.
Resumen de: CN120718821A
本发明公开了一种基因工程菌及其在抗革兰氏阴性菌感染中的应用。所述基因工程菌为在出发菌株中利用细菌表面展示系统过表达菌毛黏附素、肠道消化酶识别位点和细菌素。本发明的基因工程菌能在肠道中肠激酶的作用下，释放有活性的细菌素并暴露出菌毛黏附素，并实现杀灭肠道内的鼠伤寒沙门氏菌，及预防鼠伤寒沙门氏菌的再次定植的效果。
Resumen de: MX2025009500A
The invention relates to the field of vaccine compositions. The invention more particularly relates to a prophylactic vaccine composition that is intended for mammals and birds and comprises a killed whole bacterium, said bacterium being covered with a cationic agent, in particular cationic nanoparticles.
Resumen de: WO2025199547A1
A composition and method for therapeutic or prophylactic treatment of birds includes a mixture of IgY antibodies derived from one or more eggs laid by one or more avian species, wherein each of the one or more avian species have been vaccinated with one or more of the plurality of antigens. The mixture of IgY antibodies can be combined with a protective material that includes colostrum.
Resumen de: US2025295747A1
The present disclosure provides compositions and methods for making and using Salmonella recombinant bacteria that express LPS O78 antigen and/or virulence factors of avian pathogenic Escherichia coli (APEC) as vaccines to prevent colibacillosis infections.
Resumen de: US2025295770A1
A composition and method for therapeutic or prophylactic treatment of birds includes a mixture of IgY antibodies derived from one or more eggs laid by one or more avian species, wherein each of the one or more avian species have been vaccinated with one or more of the plurality of antigens. The mixture of IgY antibodies can be combined with a protective material that includes colostrum.
Resumen de: WO2025199081A1
The present disclosure provides compositions and methods for making and using Salmonella recombinant bacteria that express LPS O78 antigen and/or virulence factors of avian pathogenic Escherichia coli (APEC) as vaccines to prevent colibacillosis infections.
Resumen de: EP4621058A1
The present invention relates to a DNA construct including: a gene encoding a flagellin protein; and a gene encoding an immunoenhancer (adjuvant) protein. For effective cancer therapy by selectively killing only cancer cells, an attenuated Salmonella strain according to the present disclosure is designed to produce immunogenic substances in cancer tissue to induce a strong anti-cancer immune response, whereby tumor sizes in metastatic cancers as well as primary cancers can be significantly inhibited. Thus, the strain can be advantageously used in a prophylactic or therapeutic composition for improving survival rates.
Resumen de: WO2024180083A1
The present invention relates to a non-viable bacterium cell wherein an immunogenic polypeptide fused to an autotransporter comprising transmembrane linker and a trans- porter domain is displayed on the surface of the cell, and to a preparation comprising such non-viable cells. The preparation is useful as an oral vaccine.
Resumen de: AU2024217948A1
The invention relates to protein bacteriocins (PBs) as therapeutic agents, and specifically to protein complexes comprising two or more PB molecules associated with a protein scaffold which comprises cognate immunity protein domains for the effector portions of the respective PBs. In particular, the invention provides an anti-bacterial protein complex comprising (a) a first PB molecule and a second PB molecule; and (b) an immunity protein scaffold comprising a first immunity protein domain and a second immunity protein domain; wherein the first and second immunity protein domains are non-covalently bound to the respective first and second PB molecules.
Resumen de: WO2025189290A1
The present invention relates to the use of bacteria, such as Salmonella, as a carrier for an expression vector that encodes all or a portion of replicon proteins from a positive stranded virus, wherein expression of the replicon proteins is under the control of eukaryotic and/or prokaryotic promoters, and wherein expression of a payload is under the control of a sub-genomic promoter. Also provided are methods of using the bacteria comprising the vector in therapeutics and vaccines.
Resumen de: CN120622690A
本发明涉及生物技术处理污水的技术领域，尤其涉及一种含磷污水的处理方法。所述方法包括以下步骤：1）调节含磷污水的pH值为7.0‑7.5，补充乙酸钠和铵盐至浓度分别为0.1‑0.2g/L和0.01‑0.04g/L；2）在上述污水中接种聚磷菌，接种浓度为2‑5%；3）对污水中的磷污染物进行检测，符合排污标准后，排出污水。本发明对微生物进行改造，显著提高了菌体体内多聚磷酸物的水平，其改造相较于野生型提升了87.6%；导入RcsA基因后对于其荚膜产量有明显提升，而且将重度磷污染的污水中磷的含量降低到8.4mg/L，为其在商业中使用奠定了基础。
Resumen de: CN120605324A
本发明涉及一种猪源细菌多糖‑病毒蛋白结合疫苗及其制备方法和应用，属于动物疫苗技术领域。该疫苗以猪源细菌多糖和病毒蛋白为原料，通过生物素‑亲和素体系偶联形成多糖‑病毒蛋白结合物；其中病毒蛋白选自猪瘟病毒E2蛋白、猪圆环病毒2型Cap蛋白、猪伪狂犬病毒gD蛋白等蛋白中的一种，猪源细菌多糖选自猪链球菌荚膜多糖、猪多杀性巴氏杆菌荚膜多糖等多糖中的一种。该疫苗能同时诱导机体产生针对猪源细菌荚膜多糖和病毒蛋白的高水平IgG抗体，实现对猪源细菌和病毒感染的协同免疫保护，具有广谱交叉免疫效果。
Resumen de: KR20240015036A
The present invention relates to a recombinant expression vector for secretion of ifn-03b2# protein and an attenuated salmonella strain transformed by the same. provided are a recombinant expression vector comprising a flgm gene, an ifn-03b2# gene, and a flhdc gene, an attenuated salmonella strain transformed by the same, and a pharmaceutical composition for treating cancer comprising the attenuated salmonella strain as an active ingredient.
Resumen de: US2025277250A1
The invention relates to a gelled reaction medium for the detection, identification, enumeration and/or isolation of at least one target microorganism in a simple that may contain same, comprising at least one binding partner specific to a component of a target microorganism or of a component derived from said microorganism, coupled to at least one nanoparticle to form at least one binding conjugate.
Resumen de: KR20250131847A
본 발명은 대장균 및 살모넬라 균을 동시에 감염하며, 생물막 제거와 병원체 오염 식품 제어에 효과적인 용균성 박테리오파지 vB_ESM-pEJ01에 관한 것으로, 본발명에 따른 파지 및/또는 조성물은 시가 독소 생성 대장균을 제거하기 위한 항균제로 이용될 수 있다.
Resumen de: AU2024220779A1
The invention relates to the field of vaccine compositions. The invention more particularly relates to a prophylactic vaccine composition that is intended for mammals and birds and comprises a killed whole bacterium, said bacterium being covered with a cationic agent, in particular cationic nanoparticles.
Resumen de: MX2025009500A
The invention relates to the field of vaccine compositions. The invention more particularly relates to a prophylactic vaccine composition that is intended for mammals and birds and comprises a killed whole bacterium, said bacterium being covered with a cationic agent, in particular cationic nanoparticles.
Resumen de: CN120550103A
一种基于细菌外膜囊泡的口服疫苗制备方法及其应用，可有效应用于制备预防或治疗肠道黏膜病原体感染的药物，克服传统注射型疫苗依从性差、黏膜免疫反应低的问题。首先制备粪产碱杆菌的细菌外膜囊泡，然后制备DSPE‑PEG‑antigen，将两者混合后搅拌，制得OMV‑DSPE‑PEG‑antigen，取菊粉溶于PBS中，加热振荡，冷却至室温，加入OMV‑DSPE‑PEG‑antigen，搅拌，放置，得成品口服疫苗。制备工艺简单，方法稳定可靠，易操作，生产成本低，制备的基于细菌外膜囊的口服疫苗可有效激发肠道黏膜免疫应答，预防黏膜病原体的感染，经济和社会效益显著。
Resumen de: CN120569193A
The composition containing the arachidonic acid ethanolamine or the derivatives of the arachidonic acid ethanolamine is used for preparing nutritional supplements, health-care products, foods and beverages for regulating intestinal flora, the level of a first intestinal microbiota in a subject can be remarkably improved, meanwhile, the level of a second intestinal microbiota in the subject is reduced, and then intestinal health is improved. The first intestinal microbiota is selected from one or more of the following groups: Rosella, Bacteroides and Prevotella; the second intestinal microbiota is salmonella or escherichia coli.
Resumen de: CN120567452A
The invention provides a salmonella drug resistance monitoring data security management system based on a block chain, and belongs to the technical field of data transmission management. A hierarchical encryption mechanism is constructed, the technical characteristics of the block chain are fused, a standardized feature combination template architecture is designed, and a matched hierarchical transmission and intelligent contract verification system is used; a differential encryption strategy is implemented on the basis of data sensitivity, and the security of each level of data is guaranteed in combination with symmetric and asymmetric encryption technologies; through characteristic parameter modularization recombination and template association identification, data integrity is guaranteed, transmission efficiency is optimized at the same time, and scattered data can be precisely restored into a structured data set; and finally, through consensus node verification driven by an intelligent contract and on-chain state updating, whole-process standardized management and control of data from collection, transmission to storage are realized, and a salmonella drug resistance monitoring data security management mode with security, efficiency and traceability is constructed.
Resumen de: CN120555287A
The invention discloses a lactobacillus plantarum RT1 with an anti-inflammatory effect and application of the lactobacillus plantarum RT1. The lactobacillus plantarum RT1 with the anti-inflammatory effect is screened by utilizing a J774-Dual macrophage inflammation model, the influence of the RT1 on the cell activity is evaluated, and the anti-inflammatory effect of the lactobacillus plantarum RT1 is researched. It is found that the lactobacillus plantarum RT1 significantly inhibits LPS and salmonella induced NF-kappa B activation, inhibits expression of pro-inflammatory cytokines IL-1beta, TNF-alpha and IL-6, meanwhile up-regulates the expression level of an anti-inflammatory cytokine IL-10, and maintains good cell activity; dSS-induced weight loss, occult blood, colon shortening and DAI score are significantly relieved, the MPO level and inflammatory cytokine expression are reduced, and colon tissue pathological damage is improved. The lactobacillus plantarum RT1 has the function of inhibiting intestinal inflammation, so that a new material is provided for the development of novel efficient anti-inflammatory probiotics.
Resumen de: CN120555369A
The invention discloses expression and application of an Escherichia coli phage and endolysin thereof, and belongs to the technical field of microorganisms, the Escherichia coli phage VB-EcoM-WM is separated from diarrhea cattle manure of a certain cattle farm in Sichuan, the Escherichia coli phage VB-EcoM-WM is preserved in China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 2025810, the endolysin of the Escherichia coli phage VB-EcoM-WM has broad-spectrum lysing property on bacteria, and the endolysin of the Escherichia coli phage VB-EcoM-WM has broad-spectrum lysing property on bacteria. The endolysin is subjected to hydrophobic modification and prokaryotic expression to obtain the fused endolysin protein, so that the cleavage property of the endolysin is more broad-spectrum and efficient. The gene engineering modified fusion protein not only can crack host escherichia coli, but also has a bacteriostatic effect on 15 tested bacteria such as salmonella, streptococcus, staphylococcus and pasteurella. The minimum inhibitory concentration on escherichia coli is 0.1 mu g/ml. The traditional antibiotics can be replaced.
Resumen de: CN120555263A
The invention discloses a microbiological treatment flora for treating odor of a household garbage treatment station. Comprising the following microbial treatment flora components: 12 to 33 percent of biphasic photosynthetic bacteria, 0.2 to 1.5 percent of clostridium butyricum, 8 to 22 percent of bifidobacterium, 0.2 to 1.5 percent of nitrogen-fixing bacteria, 9 to 18 percent of mesophilic actinomycetes, 0.3 to 2 percent of potassium bacteria, 13 to 23 percent of subdigestion bacteria, 0.3 to 2 percent of phosphate solubilizing bacteria, 18 to 24 percent of bacillus megatherium, 0.5 to 3 percent of lactic acid bacteria and 0.5 to 3 percent of saccharomycetes. Fatty acids and sulfides are decomposed through the biphase photosynthetic bacteria through photosynthesis, stink precursor substances are reduced from the source, bifidobacteria and lactic acid bacteria reduce the pH of the environment through acid production, growth of stink-producing putrefying bacteria such as escherichia coli and salmonella is inhibited, release of stink gases such as ammonia and methyl mercaptan is reduced, saccharomycetes generate alcohol through saccharides, and the alcohol content is reduced. The removal rate of hydrogen sulfide and ammonia is greater than or equal to 80%, and the degradation rate of the volatile fatty acid is greater than or equal to 70%.
Resumen de: CN120539398A
The invention belongs to the technical field of food microorganism detection, and provides a lateral flow chromatography kit for high-sensitivity quantitative detection of salmonella enteritidis, which comprises a salmonella enteritidis RPA primer group and a lateral flow chromatography test strip, raman label streptavidin, a Digoxin antibody and a streptavidin antibody are coated on the lateral flow chromatography test strip. The reporter probe is combined on the release pad of the flow measurement chromatography test strip, has two characteristics of colorimetry and SERS (Surface Enhanced Raman Scattering), can finish simultaneous qualitative and quantitative detection of pathogenic bacteria, and improves the detection sensitivity; and the Digoxin antibody and the streptavidin antibody are respectively combined on the detection line and the quality control line. The kit does not need to use large experimental equipment in the whole detection process, is high in detection accuracy and good in specificity, and is very suitable for real-time detection under the condition of relatively low development level.
Resumen de: CN120536277A
The invention discloses a biological bacteriostatic agent and a preparation method thereof, and the preparation method comprises the following steps: step one to step three, inoculating lactic acid bacteria into a liquid fermentation culture medium, and carrying out three-stage fermentation culture; step 4, after the third-stage seed fermentation is finished, performing high-speed centrifugation on the fermentation liquid to obtain a bacterial sludge compound; 5, uniformly mixing the bacterial sludge compound with a protective agent, and performing freeze drying to obtain microbial freeze-dried powder; and 6, uniformly mixing the microbial freeze-dried powder, the auxiliary agent and the auxiliary factor to obtain the biological bacteriostatic agent. The biological bacteriostatic agent prepared by the method disclosed by the invention has remarkable bacteriostatic and bactericidal effects on various pathogenic bacteria, such as escherichia coli, salmonella and staphylococcus aureus; and main antibacterial substances come from lactic acid bacteria and metabolites thereof, so that pathogenic bacteria do not generate drug resistance to the antibacterial substances. The natural components are adopted for fermentation preparation, no chemical component is contained, the environment-friendly effect is achieved, and safety is high; and the preparation method is simple and easy for industrial production.
Resumen de: CN120514728A
The invention discloses application of synanthrin in improving the action efficiency of ampicillin on drug-resistant salmonella, and relates to the technical field of medical biology. Researches show that synanthrin does not have an inhibiting effect on salmonella, but synanthrin and ampicillin are used together, the inhibiting effect of ampicillin on salmonella can be remarkably improved, and same conclusions are drawn by in-vitro tests and in-vivo tests. According to the invention, the use of antibiotics can be reduced, and a new research direction is provided for the prevention and treatment of salmonella.
Resumen de: CN120518775A
The invention relates to the technical field of biology, in particular to fusion protein, a construction method and application. A new fusion gene is constructed by adopting an SOE-PCR technology for the first time, the fusion protein H3-OtTx1a is obtained by expression in a suitable expression system, the functions of parent proteins of the fusion protein H3-OtTx1a are integrated, the antibacterial activity of the fusion protein H3-OtTx1a is higher than that of hermetia illucens antibacterial peptide H3 and spider peptide OtTx1a, and the fusion protein H3-OtTx1a has good thermal stability, acid-base stability and endogenous protease degradation resistance, and can be used for preparing the antibacterial peptide H3 and spider peptide OtTx1a. Growth of enterobacter, salmonella and staphylococcus aureus can be obviously inhibited, and the compound has wide application prospects in the fields of food, hygienic products, cosmetics, biopesticides, biological feed additives or natural food preservatives and the like.
Resumen de: CN120514781A
The invention relates to the technical field of application of natural active substances, in particular to application of tea polyphenol in yellow tea in inhibition of bacteria, gram-positive bacteria comprise staphylococcus aureus, bacillus subtilis, enterococcus faecalis and enterococcus faecium. The gram-negative bacteria comprise escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, proteus mirabilis, salmonella and acinetobacter baumannii. The bacteriostatic activity and related mechanism of the tea polyphenol in the yellow tea are systematically studied, the bacteriostatic effect of the tea polyphenol in the yellow tea on gram-negative bacteria and gram-negative bacteria is proved, and the bacteriostatic mechanism of the tea polyphenol in the yellow tea on bacteria is disclosed through bacteriostatic experiments of the tea polyphenol on different bacteria, so that the bacteriostatic effect of the tea polyphenol in the yellow tea on the gram-negative bacteria and the bacteriostatic effect of the tea polyphenol in the yellow tea on the gram-negative bacteria are proved. The bacteriostatic activity and related mechanisms of the tea polyphenol in the yellow tea are systematically researched, the development of potential clinical medicinal value and edible value of the tea polyphenol is facilitated, and a foundation is laid for the application of the tea polyphenol in the fields of food, medicine and the like.
Resumen de: CN120519328A
The invention belongs to the technical field of lead adsorption, and relates to lead-resistant lactobacillus salivarius Pb214 and application thereof. A new lead-resistant lactobacillus salivarius Pb214 is separated from cecum contents of healthy broilers, the classification name of the lead-resistant lactobacillus salivarius Pb214 is Lactobacillus salivarius, the preservation number of the lead-resistant lactobacillus salivarius Pb214 is CGMCC (China General Microbiological Culture Collection Center) NO.33992, and the lead-resistant lactobacillus salivarius Pb214 is preserved in China General Microbiological Culture Collection Center on March 27, 2025. Meanwhile, experiments prove that the maximum tolerance lead concentration of the lactobacillus salivarius is 1000mg/L, the average lead adsorption rate of the lactobacillus salivarius to a 5mg/L lead solution (pH 5) is 94.9%, the lead adsorption rate is the highest and reaches 96.33% when the pH is 7, and the lactobacillus salivarius can tolerate 0.3% cholate and also can inhibit the growth of salmonella pullorum and staphylococcus aureus. Therefore, the lead-resistant lactobacillus salivarius can be used for removing the lead metal element in a matrix, and the matrix can be soil, feed, a culture medium and other substances needing lead removal.
Resumen de: WO2024155027A1
The present invention relates to an attenuated Salmonella gallinarum expressing FliC or FliC-hiL2 and use thereof. The Salmonella strain according to the present invention has excellent immune activity and exhibits excellent anti-cancer efficacy, and thus can be used as a therapeutic agent for cancer together with or independently of existing anti-cancer drugs.
Resumen de: CN120522381A
The invention provides a kit for detecting shigella by a Marker method, and the Marker method represents fluorescence intensity corresponding to shigella with different concentrations and is a basis for establishing a Marker graph; the kit comprises a Marker graph, a magnetic aptamer, a detection aptamer, a bacterial lysis solution, an enzyme digestion solution, a binding solution, a washing solution and a magnetic frame. The detection method comprises the following steps: the kit is used for splitting the shigella through a bacterial lysis solution to release DNA, the DNA is cut through an enzyme digestion solution to obtain a fragment rich in an ipaH gene, the magnetic aptamer and the detection aptamer are specifically combined with the ipaH fragment under the action of a binding solution to form a compound, the compound is washed with washing liquid, the fluorescence intensity is detected and compared with a Marker diagram, and quantitative detection of the shigella is realized. The method does not need to treat a sample in advance, shortens the detection time, and has strong specificity and high sensitivity.
Resumen de: CN120522382A
The invention discloses a nano-enzyme composite material for detecting pathogenic microorganisms in a complex matrix and application of the nano-enzyme composite material, and belongs to the technical field of pathogen detection. The kit containing the nano-enzyme composite material provided by the invention can realize accurate detection of food-borne pathogenic bacteria in a complex matrix, and has an excellent visual detection effect. And the environment-stable storage performance is excellent and can be up to 60 days. Excellent catalytic activity, stability and selectivity are shown in the aspect of detecting escherichia coli, staphylococcus aureus, vibrio parahaemolyticus and salmonella typhimurium. According to the invention, rapid and reliable detection can be realized, and the detection limit of different pathogens in a PB buffer solution (pH 7.2) is 2-7CFU/mL.
Resumen de: CN120518710A
The invention belongs to the technical field of biology, and particularly discloses an antibacterial peptide Meijia-1 and application thereof. The amino acid sequence of the antibacterial peptide is as follows: arginine, glycine, arginine, phenylalanine, glycine, arginine, phenylalanine, leucine, lysine, lysine, valine, arginine, arginine, phenylalanine, isoleucine, proline, lysine and valine. The antibacterial peptide Meijia-1 has better antibacterial activity on gram-positive bacteria (staphylococcus aureus) and gram-negative bacteria (escherichia coli and salmonella), and has the characteristics of heat resistance, salt resistance, acid and alkali resistance, repeated freezing and thawing resistance, serum resistance, organic solvent resistance and pepsin resistance. Therefore, the antibacterial peptide Meijia-1 has wide application prospects in the fields of pathogenic infection resistance, animal feed, food preservation and preservatives, cosmetics, detergents, disinfectants, pesticides, antibacterial molecular materials and the like.
Resumen de: US2025261659A1
Two stage heated water treatment of shell eggs results in-shell poached eggs. The in-shell poached are sufficiently cooked so when the eggs are cracked onto a plate the eggs have the characteristics of conventional poached eggs without further cooking and need only to be warmed for consumption. The in-shell poached eggs are also pasteurized in the shell to meet the FDA and WHO requirement of a 5-log reduction of Salmonella Enteritidis.
Resumen de: WO2025171792A1
Provided is use of a combination of three or more of the following microorganisms in the preparation of an anti-tumor combination vaccine: Bordetella pertussis, Salmonella typhi, Salmonella paratyphi A, Salmonella paratyphi B, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, Proteusbacillus vulgaris, Lactic acid bacteria, Bifidobacterium longum, Bordetella pertussis, Corynebacterium diphtheriae and Clostridium tetani, Clostridium acetobutylicum, Salmonella typhimurium, and Streptococcus pyogenes. The anti-tumor combination vaccine is broad-spectrum, safe and non-toxic, and thus has the prospect of clinical applications.
Resumen de: WO2025175111A1
Two stage heated water treatment of shell eggs results in-shell poached eggs. The in-shell poached are sufficiently cooked so when the eggs are cracked onto a plate the eggs have the characteristics of conventional poached eggs without further cooking and need only to be warmed for consumption. The in-shell poached eggs are also pasteurized in the shell to meet the FDA and WHO requirement of a 5-log reduction of Salmonella Enteritidis.
Resumen de: KR20240015036A
The present invention relates to a recombinant expression vector for secretion of ifn-03b2# protein and an attenuated salmonella strain transformed by the same. provided are a recombinant expression vector comprising a flgm gene, an ifn-03b2# gene, and a flhdc gene, an attenuated salmonella strain transformed by the same, and a pharmaceutical composition for treating cancer comprising the attenuated salmonella strain as an active ingredient.
Resumen de: KR20240015036A
The present invention relates to a recombinant expression vector for secretion of ifn-03b2# protein and an attenuated salmonella strain transformed by the same. provided are a recombinant expression vector comprising a flgm gene, an ifn-03b2# gene, and a flhdc gene, an attenuated salmonella strain transformed by the same, and a pharmaceutical composition for treating cancer comprising the attenuated salmonella strain as an active ingredient.
Resumen de: CN120051482A
The present invention relates to a modified live attenuated Gram-negative bacterium wherein the bacterium has been modified such that an RNA molecule can be safely and efficiently delivered to a target eukaryotic cell. Thus, the present invention relates to a bacterial delivery system and various uses and methods thereof.
Resumen de: CN120504728A
The invention discloses an application of a polypeptide in inhibiting bacteria or preparing a reagent for inhibiting bacteria, and belongs to the technical field of biology. According to preferred codons of bacillus subtilis, a polypeptide coding gene which is derived from myxobacteria and has unknown functions is designed, and a nucleotide sequence is obtained. And then carrying out recombinant expression in bacillus subtilis by utilizing a genetic engineering technology. The fermentation liquor of the recombinant bacillus subtilis shows better antibacterial activity on gram-positive bacteria represented by staphylococcus aureus, pseudomonas aeruginosa, salmonella, escherichia coli and gram-negative bacteria represented by vibrio parahaemolyticus. The antibacterial function of the polypeptide in myxobacteria is found for the first time, namely, the novel antibacterial peptide is provided, and compared with traditional antibiotics, the novel antibacterial peptide is equivalent or higher in antibacterial activity, wide in antibacterial spectrum and low in preparation cost and has wide application prospects in the fields of antibacterial preparations, food preservatives and the like.
Resumen de: CN120505281A
The invention discloses a hybridoma cell strain secreting an anti-salmonella abortus groEL protein monoclonal antibody, the monoclonal antibody and application of the hybridoma cell strain. The hybridoma cell strain is named as E11, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the strain preservation number of the hybridoma cell strain is CGMCC No.46349. According to the present invention, the salmonella universal cELISA antibody detection method capable of being applied to different animals is established by using the E11 MAb secreted by the hybridoma cell strain secreting the anti-horse salmonella abortus groEL protein monoclonal antibody and the horse salmonella abortus groEL protein; the salmonella comprises salmonella abortus (S.Abortuqui), salmonella typhimurium (S.Typhi), salmonella dublin (S.Dublin) and salmonella enteritidis (S.Enteridis), so that the method disclosed by the invention has the advantages of good specificity and broad spectrum. The invention provides an effective technical means for diagnosis, prevention and control of salmonellosis of different animals clinically.
Resumen de: CN120505437A
The invention relates to the technical field of salmonella detection, in particular to a salmonella molecular detection method and application thereof, and the salmonella molecular detection method comprises the following steps: S1, sample treatment: extracting DNA in a to-be-detected sample; step S2, primer design and synthesis: specific primers are designed for the invA gene, the hilA gene and the stn gene of salmonella; s3, multiple PCR reaction: taking the DNA extracted in the step S1 as a template, and carrying out multiple PCR amplification by adopting the primer in the step S2; s4, fluorescent probe hybridization: hybridizing a PCR amplification product in the step S3 with a fluorescent labeled probe; s5, fluorescence signal detection: detecting a fluorescence signal after hybridization through a fluorescence detector, and judging whether the sample contains salmonella or not according to the signal. According to the salmonella molecular detection method provided by the invention, a technology of combining multiple PCR with fluorescent probe hybridization is adopted, a plurality of conserved genes of salmonella can be detected at the same time, and the detection specificity and sensitivity are improved.
Resumen de: CN120505321A
The invention relates to the technical field of salmonella detection, in particular to a multivalent aptamer probe for capturing salmonella, a preparation method of the multivalent aptamer probe and application of the multivalent aptamer probe to salmonella detection. The invention relates to a multivalent aptamer probe for capturing salmonella. The multivalent aptamer probe is prepared from a DNA sample H1, a DNA sample H2, an activation chain, an aptamer and cDNA dry powder through a hybridization chain reaction technology. The multivalent aptamer probe is used in the salmonella detection process, and has the advantages of one-tube detection, no secondary pollution and high detection sensitivity.
Resumen de: CN120505438A
The invention discloses a composition and method for detecting food-borne pathogenic bacteria and application, and belongs to the technical field of food safety detection. The gDNAs and fluorescent probe combination suitable for multi-target parallel detection is obtained through multiple rounds of screening, meanwhile, the advantages of LAMP efficient amplification and PfAgo targeted cutting are combined, a multiple LAMP-PfAgo rapid detection method for three pathogenic bacteria of staphylococcus aureus, salmonella and listeria monocytogenes is established, and the problem that LAMP multiple detection is difficult is effectively solved. According to the present invention, the specificity is high, the result determination is simple, the detection limit on the pure culture can achieve 101 CFU/mL, the detection can be completed within 45 min, and the important practical application value is provided.
Resumen de: WO2025170108A1
The present invention relates to identifying and engineering a tail spike protein gene from a genome of bacteriophage SFP10 infected with a Salmonella strain, purifying and producing a recombinant protein using mSFP10TSP, which is a recombinant protein formulation for detecting Salmonella enterica, EGFP-mSFP10TSP fused with a green fluorescent protein, and SiBD-mSFP10TSP fused with an SiBD, and thereby specifically detecting the Salmonella strain.
Resumen de: CN120484082A
The invention relates to the technical field of biology, in particular to an antibacterial peptide Aquiluscidin-1, a coding gene and application of the antibacterial peptide Aquiluscidin-1. The invention provides an antibacterial peptide Aquiluscidin-1 which has good thermal stability, acid-base stability and endogenous protease degradation resistance, can obviously inhibit the growth of escherichia coli, salmonella and staphylococcus aureus, and has good application prospects in the fields of food, hygienic products, cosmetics, biopesticide, biological feed additives or natural food preservatives and the like. Wide application prospects are realized.
Resumen de: CN120484134A
The invention relates to the technical field of biology, in particular to hermetia illucens antibacterial peptide H3 fusion protein, a construction method and application. The novel hermetia illucens antibacterial peptide H3 fusion protein provided by the invention not only has the antibacterial activity of the antibacterial peptide, but also has the activity of glucoside hydrolase, can realize rapid visual monitoring of the activity of the antibacterial peptide, has an inhibition effect on escherichia coli, salmonella and staphylococcus aureus, and can be used for preparing the antibacterial peptide H3 fusion protein. In the fields of food, hygienic products, cosmetics, biopesticides, biological feed additives or natural food preservatives and the like, the method has a wide application prospect.
Resumen de: CN120478388A
The invention discloses antibacterial application of mulberry polysaccharide, and the mulberry polysaccharide is prepared by the following method: drying fresh mulberry, crushing, and adding into absolute ethyl alcohol for extraction; then taking the precipitate and drying; adding into distilled water, performing water bath, centrifuging, taking supernate, performing vacuum concentration, centrifuging again, taking precipitate, adding distilled water into the precipitate, redissolving, and drying to obtain mulberry polysaccharide; the mulberry polysaccharide obtained by the method has a relatively good inhibition effect on gram-negative bacteria escherichia coli and salmonella and gram-positive bacteria staphylococcus aureus; the antibacterial effect is enhanced along with the rise of the temperature, so that the heat stability is achieved, and meanwhile, the good antibacterial effect is achieved in both acid and alkali environments; the mulberry polysaccharide can be further prepared into a novel natural bacteriostatic agent and has a wide application prospect in the fields of daily chemicals and medicines.
Resumen de: CN120478554A
The invention relates to the technical field of animal medicines, and particularly discloses a veterinary medicine compound preparation as well as a preparation method and application thereof. The veterinary medicine compound preparation is prepared by boiling, extracting, concentrating and diluting eight Tibetan medicines including aconitum pendulum, fructus chebulae, radix inulae inula, benzoin, pterocephalus hookeri, lagotis lagotis, amomum tsao-ko and rhodiola rosea. The veterinary drug compound preparation provided by the invention has an antibacterial range of 8.67 mm to 23.77 mm on enterotoxic large intestine dried bacteria, salmonella enteritidis and pasteurella multocida, and has an inhibition rate of 60% to 72% on enterotoxigenic escherichia coli diarrhea, salmonella enteritidis diarrhea and pasteurella multocida diarrhea; therefore, the veterinary drug compound preparation provided by the invention has a remarkable inhibition effect on enterotoxic large intestine dry bacteria, salmonella enteritidis and pasteurella multocida.
Resumen de: CN120484133A
本发明提供了一种基于猪圆环病毒2型(PCV2)纳米抗体与沙门氏菌鞭毛蛋白的重组融合蛋白及其制备方法与应用。本发明将PCV2衣壳蛋白(Cap)特异性纳米抗体基因与沙门氏菌鞭毛蛋白基因融合，使用大肠杆菌原核表达系统表达制备了Nbcap‑flagellin重组融合蛋白。该重组融合蛋白能够利用Cap蛋白特异性纳米抗体的靶向结合能力，实现鞭毛蛋白佐剂与抗原蛋白的动态偶联，形成佐剂用量可调控的抗原‑佐剂复合物；在充分发挥鞭毛蛋白作为免疫佐剂作用的同时，突破传统鞭毛蛋白与抗原蛋白融合表达导致的固定佐剂‑抗原配比限制，提升了疫苗设计的灵活性，避免了细菌鞭毛蛋白过量可能引发的炎性反应，为开发高效、安全且广谱的PCV2亚单位疫苗提供了新的技术路径。
Resumen de: CN120485133A
The invention discloses a salmonella bacteriophage Y1 with a wide lysis spectrum and a preparation method of a low-endotoxin preparation of the salmonella bacteriophage Y1. The preservation number of the salmonella bacteriophage Y1 is CCTCC M 20242911. The method comprises the following steps: adding a chitosan solution into a bacteriophage suspension, uniformly mixing, and precipitating overnight at room temperature. The salmonella phage Y1 disclosed by the invention is high in cracking capacity and can be applied to preparation of drugs for preventing and treating salmonella. The method disclosed by the invention is low in cost, simple in used equipment, simple and convenient in operation procedure, stable in effect, easy for large-scale industrial production, low in endotoxin preparation 3, good in endotoxin removal effect and small in influence on the activity of the bacteriophage.
Resumen de: WO2025170108A1
The present invention relates to identifying and engineering a tail spike protein gene from a genome of bacteriophage SFP10 infected with a Salmonella strain, purifying and producing a recombinant protein using mSFP10TSP, which is a recombinant protein formulation for detecting Salmonella enterica, EGFP-mSFP10TSP fused with a green fluorescent protein, and SiBD-mSFP10TSP fused with an SiBD, and thereby specifically detecting the Salmonella strain.
Resumen de: KR20250122779A
구현예의 수의학적 조성물은 가금류의 대장균 또는 살모넬라균에 의한 설사 증상의 치료 또는 완화용도를 갖는 것으로, 색도 L이 77 이하인 액상이고, 유효성분 및 항산화제를 포함하고, 상기 유효성분은 아프라마이신 또는 이의 염을 유효성분을 포함한다. 구현예의 수의학적 조성물은 조류의 대장균증 또는 추백리의 치료 또는 완화용으로 그 효과가 우수하다. 또, 구현예의 수의학적 조성물은 아프라마이신 또는 이의 염을 유효성분으로 포함하는 액상 조성물로 유효성분에 대한 조성물의 보관 안정성이 우수하고, 투여가 용이한 장점도 있다.
Resumen de: WO2025170306A1
The present invention relates to an antibacterial composition comprising, as an active ingredient, a plantaricin peptide derived from a Lactiplantibacillus plantarum KM2 strain, wherein the plantaricin peptide derived from the strain and a plantaricin peptide combination were found to have antimicrobial activity against at least one selected from the group consisting of Flavobacterium sp, Enterococcus faecalis, Listeria monocytogenes, Staphylococcus aureus, and acromobacter xylosoxidans (Alcaligenese xylosoxidans), Salmonella enterica, <i />and Vibrio parahaemolyticus, and the plantaricin peptide and the plantaricin peptide combination were found to have antibacterial activity and a cell wall lysis effect against the Flavobacterium sp. strain, and accordingly, the plantaricin peptide derived from the Lactiplantibacillus plantarum KM2 strain and the plantaricin peptide combination are provided as antimicrobial agents.
Resumen de: MY209939A
The present invention relates to a molecular detection kit for simultaneously detecting hemorrhagic bacteria (100), comprising a PCR reaction mastermix; a primer pool that comprises a plurality of specific primer pairs of bacteria that include forward and reverse primers and an internal control; and a buffer solution; characterized in that, the internal control is of Vibrio cholera hemA gene; and a plurality of specific primers of bacteria further comprising Shigella spp. 2a plasmid pMYSH6000 (virA) gene; Campylobacter 16S ribosomal RNA (rRNA) gene; Salmonella spp. outer membrane porin C (ompC) gene; and E.coli E6-3 intimin (eaeA) gene. The method for detecting the bacteria consisting of Shigella, Campylobacter, Salmonella and E. coli (200) using the molecular detection kit (100) is also provided. Figure 1
Resumen de: CN120459124A
The invention discloses a novel application of rutin in preparation of a salmonella type III secretion system (T3SS) inhibitor. By constructing a salmonella mouse infection model, the rutin is proved to be capable of remarkably improving the survival rate of infected mice, reducing the bacterial load of liver and spleen target organs and effectively relieving the tissue damage of intestinal tracts, livers and spleens. In-vitro tests show that rutin does not affect the growth of bacteria, and the mechanism of the rutin playing a protection role is that the expression of a salmonella T3SS virulence factor is specifically inhibited, so that the pathogenicity of salmonella is weakened. The invention discloses a potential application of rutin as a salmonella T3SS inhibitor, and provides a theoretical basis for development of related anti-infective drugs.
Resumen de: CN120459191A
The invention provides a novel application of a cordate houttuynia extract as a salmonella type III secretion system (T3SS) inhibitor. Through construction of a mouse infection model and in-vitro experiments, the extract is proved to be capable of remarkably reducing the bacterial load of infected mouse liver and spleen tissues, relieving tissue pathological damage and reducing inflammatory cell infiltration. Mechanism research shows that the Houttuynia cordata extract can down-regulate the expression of T3SS key virulence protein SipA in a dose-dependent manner, interfere the function of a secretion system, and further block the secretion and expression of virulence factors. The potential application of the herba houttuyniae extract in the aspect of resisting salmonella toxicity is disclosed for the first time, and a development strategy and a technical basis of a T3SS inhibitor derived from a natural product are provided.
Resumen de: CN120464522A
The invention discloses bacillus subtilis capable of strongly inhibiting clostridium perfringens and relates to the technical field of microorganisms, the bacillus subtilis is preserved in China General Microbiological Culture Collection Center on November 25, 2024, the preservation number is CGMCC No.32783, the bacillus subtilis is classified and named as bacillus subtilis, and the bacillus subtilis is preserved in the China General Microbiological Culture Collection Center on November 25, 2024. The bacillus subtilis is bacillus subtilis which can produce surfactin well and has a strong inhibition effect on clostridium perfringens, and the bacillus subtilis also has a certain inhibition effect on large intestines, salmonella and staphylococcus aureus.
Resumen de: KR20250121896A
본 발명은 사람에서 세균성 식중독을 일으키는 주요 원인균인 S. Enteritidis 및 S. Typhimurium, 그리고 가금에서 폐사를 일으키는 가금티푸스 원인균인 S. Gallinarum에 대한 산란계에서의 효과적인 살모넬라균 감염 예방을 위한 최적의 예방 프로그램 확립을 위한 발병을 수행하였다. 약독화 생균 백신만을 접종한 경우와 3가 살모넬라 불활화 사균체 백신만을 단독 접종한 산란계 모두에서 폐사는 발생하지 않았지만, 내부 장기 및 맹장 그리고 총 배설강 등에서 도전감염 균주가 분리되었다. 하지만 시판 백신인 약독화 생균 백신인 SG9R을 1차로 피하 접종한 후, 3가 살모넬라 불활화 사균체 (살모넬라 엔터라이티디스, 살모넬라 타이피뮤리움, 살모넬라 갈리나룸 불활화 사균체) 백신으로 추가로 근육 접종할 경우에는 내부 장기 및 맹장과 총배설강 등지에서 도전감염 균주가 분리되지 않았다. 이를 통해 약독화 생균 백신 및 3가 살모넬라 불활화 사균체 백신을 단독으로 접종한 경우보다 약독화 생균 백신 접종 후, 3가 살모넬라 불활화 사균체 백신을 추가로 접종할 경우 산란계에서 주요 살모넬라균을 효과적으로 예방할 수 있음이 확인하였다.
Resumen de: KR20250121172A
E. coli O157:H7을 감염하는 두 종의 박테리오파지 SFP10과 PhiV10로부터 얻은 tail spike protein(TSP)를 엔지니어링 하여 항체를 사용하지 않고도 식품 내 E. coli O157:H7균을 신속하게 검출할 수 있는 방법 및 이의 방법으로 제작된 재조합 단백질이다.
Resumen de: CN120464710A
The invention discloses a screening culture medium and a preparation method and application thereof, the screening culture medium comprises a basic culture medium and additives, and the additives comprise amphotericin B, vancomycin, ox bile salt, sodium deoxycholate and No.3 bile salt. According to the screening culture medium provided by the invention, a special infectious microbe inhibitor is added on the basis of a trisaccharide iron culture medium, so that most gram-negative enterobacter, staphylococcus aureus and part of fungi except salmonella enteritis subspecies and shigella cannot grow; the interference of the infectious microbes on the detection of the salmonella enteritis subspecies and the shigella is avoided, so that the fluorescent probe can be applied to the detection of the salmonella enteritis subspecies and the shigella in a clinical anus swab, and the sensitivity of color development is improved.
Resumen de: CN120468246A
The invention provides a magnetic control type electrochemical biosensor of MWCNTs-MoS2 based on CRISPR (clustered regularly interspaced short palindromic repeats)/Cas12a as well as a preparation method and application of the magnetic control type electrochemical biosensor. The magnetic control type electrochemical biosensor comprises an aminated multi-walled carbon nanotube-molybdenum disulfide modified screen-printed carbon electrode, wherein a CRISPR-crRNA-Target DNA ternary complex and a ferroferric oxide-gold-single-stranded nucleotide probe are dropwise added on the surface of the screen-printed carbon electrode, and the probe is sheared after the reaction of the CRISPR-crRNA-Target DNA ternary complex and the ferroferric oxide-gold-single-stranded nucleotide probe; the magnetic control type electrochemical biosensor respectively shows wide linear response in the range of 4.6 * 10 < 1 >-4.6 * 10 < 7 > CFU/mL of staphylococcus aureus and 5.5 * 10 < 7 > CFU/mL of salmonella, the detection limit of the magnetic control type electrochemical biosensor to two pathogenic bacteria is as low as 2CFU/mL, and the magnetic control type electrochemical biosensor has good specificity to interfering bacteria, can be used for rapid detection of food safety and has good practicability.
Resumen de: CN120464525A
The invention discloses a bacillus cereus JBH-LY1 strain and an application of the bacillus cereus JBH-LY1 strain in aquaculture, and relates to the technical field of microorganisms. The bacillus cereus JBH-LY1 is preserved in the China General Microbiological Culture Collection Center on June 6, 2024, and the preservation number of the bacillus cereus JBH-LY1 is CCTCC (China Center for Type Culture Collection) NO: M 20241168. The bacillus cereus is natural, safe, acid-resistant, alkali-resistant and high-temperature-resistant, has strong capability of producing protease, amylase, lipase and cellulase, and has a bacteriostatic effect on escherichia coli, salmonella typhimurium, staphylococcus aureus and aeromonas hydrophila. The bacillus cereus is used in the aquaculture industry, can purify aquaculture water and reduce the content of nitrogen and phosphorus, and also can regulate intestinal flora balance of cultured animals, promote growth, improve disease resistance and increase culture benefits.
Resumen de: CN120436313A
The invention provides a portulaca oleracea fermentation process, which utilizes lactobacillus plantarum BJ-M8 with carbohydrate amylase, glucosidase and cellulase production capacity to ferment portulaca oleracea, and promotes the conversion of macromolecular glycoside substances to small molecular aglycones. Metabonomics analysis shows that through fermentation treatment of the strain BJ-M8, the composition of alkaloids, phenolic acids, flavonoids and the like of purslane is effectively improved. Compared with the prior art, the target product calycosin is increased by 10 times, acacetin is increased by 7 times, kaempferol is increased by 4 times, apigenin is increased by 4 times, naringenin is increased by 3 times, dihydroferulic acid is increased by 177 times, dihydrocaffeic acid is increased by 27 times, and spinogenin is increased by 6 times. According to an in-vitro evaluation test, the inhibition effect of the fermented purslane on salmonella and escherichia coli is enhanced, the anti-inflammatory effect on LPS treated macrophage RAW264.7 and the oxidation resistance on Caco-2 cells are improved, and the application prospect in the aspect of functional product preparation is wide.
Resumen de: CN120442560A
The invention relates to the technical field of salmonella bacteriophages, and in particular relates to a bacteriophage capable of splitting salmonella brassicae as well as a composition and application of the bacteriophage capable of splitting the salmonella brassicae. The bacteriophage is salmonella bacteriophage RDP-SA-22010, the preservation number of the bacteriophage is CGMCC (China General Microbiological Culture Collection Center) No.45274, and the bacteriophage is preserved in the China General Microbiological Culture Collection Center on September 21, 2022. The salmonella bacteriophage RDP-SA-22010 can also be used for cracking salmonella enteritidis, salmonella pullorum and salmonella kafenkii. The invention also relates to a composition, which comprises the salmonella bacteriophage RDP-SA-22010. The reagent or the kit comprises the composition. The invention also provides application of the salmonella bacteriophage RDP-SA-22010 to preparation of a biological bactericide, preparation of a medicine for treating diseases caused by salmonella and preparation of a feed additive. The salmonella bacteriophage RDP-SA-22010 disclosed by the invention provides a novel biological prevention and treatment means for the prevention and treatment of salmonella bulidan.
Resumen de: CN120442561A
The invention provides a cross-species lysis bacteriophage RDP-EC-20146 and application thereof, and belongs to the technical field of biology. The cross-species lysis bacteriophage is an escherichia coli bacteriophage, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.45351, and the cross-species lysis bacteriophage is preserved in China General Microbiological Culture Collection Center on October 28, 2022. The cross-species lysis bacteriophage RDP-EC-20146 provided by the invention has the characteristics of high lysis activity and good physical and chemical factor tolerance; the method is not influenced by the drug resistance of bacteria, does not have the problems of drug residues and the like, and has very high use safety; in addition, when the bacteriophage RDP-EC-20146 provided by the invention is combined with 8 low-dose antibiotics for use, a remarkable bacteriostatic effect can be shown, and the bacteriophage RDP-EC-20146 has a good application prospect in the aspect of preventing and treating salmonella, escherichia coli, staphylococcus aureus and/or pseudomonas aeruginosa diseases.
Resumen de: WO2025165867A1
Provided herein are compositions comprising in vivo succinate-producting microorganisms, e.g., Bacteroides and Prevotella species (preferably Bacteroides thetaiotaomicron (e.g., VPI 5482), Bacteroides vulgatus (e.g., NCTC 11154, Prevotella copri (e.g., DSM 18205)), Parabacteroides (e.g., Parabacteroides distasonis), and Bacilli (e.g. Lactobacillus animalis), and methods of use thereof in treating or reducing risk of developing an intestinal infection, optionally an infection with Campylobacter, Salmonella, E. coli, Shigella, Listeria monocytogenes, Vibrio, Enteropathogenic E. coli, Klebsiella, or Clostridioides difficile, or promoting expansion of colonic tuft cells, in a subject.
Resumen de: CN120427903A
The invention discloses a colorimetric-fluorescent dual-signal salmonella detection method based on Ag-MoS2 nano-enzyme. The method is characterized by comprising the following steps: mixing a single strand S1, a single strand S2, a single strand S3, a single strand S4, a salmonella aptamer and cDNA (complementary deoxyribonucleic acid) to prepare a multivalent aptamer competitive probe solution; respectively dissolving a hairpin probe H1 and a hairpin probe H2 in PBS (Phosphate Buffer Solution); a step of preparing an Ag-MoS2 nano enzyme composite material; the method comprises the following steps: mixing and incubating a to-be-detected salmonella solution and a multivalent aptamer competitive probe, adding the Ag-MoS2 nano-enzyme composite material for performing CHA amplification reaction, then adding an H2O2 solution and a TMB solution for performing peroxidase catalytic reaction, respectively performing colorimetric method and fluorescence method detection, and calculating to obtain the salmonella concentration in the to-be-detected solution. The kit has the advantages of simplicity, rapidness, high sensitivity, strong specificity, high accuracy and real-time sterilization after detection.
Resumen de: CN120420281A
The invention discloses florfenicol nano-liposome and a preparation method and application thereof, the florfenicol nano-liposome is prepared from soybean lecithin, florfenicol and cholesterol according to the mass ratio of (10-30): 4: 5, florfenicol, cholesterol and soybean lecithin are dissolved in an organic solvent, rotary evaporation is performed, water is added for dissolution, and then ultrasonication is performed to obtain the florfenicol nano-liposome. The average particle size of the florfenicol liposome prepared by the preparation method disclosed by the invention is 249.6 to 638.2 nm, the Zeta potential value of the florfenicol liposome is-13.7 to-46.3 mV, and the polydispersity coefficient of the florfenicol liposome is 0.22 to 0.86. The entrapment rate is 88.31%-92.94%, the drug loading capacity is 3.21%-6.29%, the minimum inhibitory concentration to a salmonella standard strain is 4 mu g/mL, and the minimum bactericidal concentration to the salmonella standard strain is 8 mu g/mL. The minimum inhibitory concentration of the salmonella drug-resistant strain is 8 mu g/mL, and the minimum bactericidal concentration of the salmonella drug-resistant strain is 34 mu g/mL.
Resumen de: KR20250117498A
본 발명은 살모넬라 균 (Salmonella spp.) 검출용 조성물 및 이를 이용한 검출방법에 관한 것으로서, 보다 상세하게는 살모넬라 균 InvA 유전자 서열을 증폭하기 위한 프라이머 세트와 프로브에 관한 것으로, 식용 갈색거저리 내 유해균인 살모넬라 균을 특이적으로 검출 할 수 있다.
Resumen de: CN120400125A
The invention discloses a method for rapidly extracting and enriching salmonella pullorum nucleic acid based on chitosan modified diatomite. The method comprises the following steps: (1) pretreating diatomite; (2) functional modification of chitosan; and (3) extracting salmonella pullorum nucleic acid. The chitosan-diatomite composite material disclosed by the invention is based on a synergistic mechanism of an electrostatic interaction micropore interception effect, the selectivity is improved by utilizing the cationic characteristic of chitosan, the chitosan is fixed on the surface of diatomite through glutaraldehyde crosslinking, the dissolution loss in an acid environment is avoided, the high specific surface area of the diatomite and the charge adsorption of the chitosan are combined, and the adsorption capacity of the diatomite is improved. A'physical interception + chemical bonding 'dual-mode enrichment mechanism is established, efficient capture of existing nucleic acid is achieved, qPCR detection sensitivity is remarkably improved, the bottleneck problem of a traditional nucleic acid extraction method in complex poultry samples is solved, and the method has the advantages of high detection rate, high anti-interference performance and operation convenience and is suitable for popularization and application. And reliable technical support is provided for rapid molecular diagnosis and epidemiological monitoring of the pullorum disease bacteria.
Resumen de: CN120400315A
The invention discloses a rapid detection method and a kit for salmonella typhimurium in a dairy product, and relates to the technical field of biological detection, the kit is provided with a primer group and a reagent, and the kit contains a positive reference substance and a negative reference substance; the detection method comprises the following steps: acquiring a sample, purifying genome DNA of the sample, carrying out PCR amplification by taking the purified genome DNA as a template to obtain a salmonella typhimurium fimY gene segment, purifying the salmonella typhimurium fimY gene segment, carrying out T-A ligation reaction, transformation and recovery, preparing a fluorescent quantitative PCR standard substance, preparing a dye-method real-time fluorescent quantitative PCR reaction system, and detecting the salmonella typhimurium fimY gene segment. Fluorescence signals are collected in real time, a Ct value is measured, a standard curve is drawn, the sample concentration is calculated, and qualitative and quantitative analysis is carried out. The method does not need to design a complex probe, the primer design is relatively simple, the detection accuracy and reliability are improved, the cost is reduced, and the detection efficiency is greatly improved.
Resumen de: CN120400005A
The invention discloses attenuated salmonella recombinant engineering bacteria for expressing TFPI-2 and IFN alpha as well as a preparation method and application of the attenuated salmonella recombinant engineering bacteria, and relates to the technical field of biology. The preparation method of the recombinant engineering bacterium comprises the following steps: sequentially connecting a secretory signal peptide coding gene, a TFPI-2 gene and an IFN alpha gene to obtain a connection product; connecting the connection product to an expression vector to obtain a recombinant plasmid; and transforming the recombinant plasmid into attenuated salmonella to obtain an attenuated salmonella recombinant engineering bacterium for expressing TFPI-2 and IFN alpha. The recombinant engineering bacterium can be colonized in a tumor tissue area, stably and continuously express and secrete TFPI-2 and IFN alpha, promotes tumor tissue immune cell infiltration, and shows good tumor treatment effect and tumor targeting.
Resumen de: CN120400381A
The invention provides a primer combination of a multiple detection kit for food-borne pathogenic bacteria and the multiple detection kit. The primer combination comprises probes and primers for detecting nine food-borne pathogenic bacteria: vibrio cholerae general type, vibrio cholerae O1 type, vibrio cholerae O139 type, vibrio parahaemolyticus, salmonella, yersinia enterocolitica, campylobacter, listeria monocytogenes and enterobacter sakakaakii, and the sequence is shown as SEQ1-31; the probe and the primer pair are used for detecting nine characteristic genes, namely a shigella universal type: ipaH, a diarrheogenic Escherichia coli universal type: eltA, sta3, sta and aggR, and enterohemorrhagic Escherichia coli: stx1, stx2, escV and rfbE, and the sequences of the probe and the primer pair are shown as SEQ32-64. According to the invention, by combining a digital PCR multiple coding technology, the detection of 12 food-borne pathogenic bacteria can be realized, and the sensitivity, the specificity and the accuracy are relatively good.
Resumen de: CN120392738A
The invention discloses an application of bilobalide in preparation of a product for resisting salmonella typhimurium infection. The bilobalide takes salmonella typhimurium QseB protein as a target, has a good interference effect on an AI-2 quorum sensing signal of salmonella typhimurium and has good pharmacological activity, and particularly, the bilobalide has good pharmacological activity under the condition that the growth of salmonella typhimurium is not inhibited. The motility, biofilm formation and infection pathogenicity of salmonella typhimurium are obviously inhibited. The bilobalide does not influence the growth of salmonella typhimurium, directly acts on pathogenic factors, weakens the pathogenicity of bacteria from the source, does not generate selective pressure on salmonella typhimurium, and does not cause the generation of drug-resistant pathogenic bacteria. Therefore, the bilobalide has a good application prospect in the aspect of development of the salmonella typhimurium infection resisting medicine.
Resumen de: CN120405121A
The invention belongs to the technical field of biochemical analysis and detection, and particularly relates to a composite nanomaterial probe for detecting salmonella typhimurium, a preparation method of the composite nanomaterial probe and a dual-mode immunochromatography test strip. According to the present invention, the improved dual-mode reading is achieved by improving the signal label, the BrM (at) Os with the surface modified with the rabbit anti-salmonella typhimurium polyclonal antibody is adopted as the detection probe, and the fluorescence and catalytic colorimetric dual-mode immunochromatography test strip capable of detecting salmonella typhimurium is prepared; the immunochromatographic test strip can realize sensitive immunochromatographic analysis on salmonella typhimurium in both fluorescence and catalytic colorimetric modes.
Resumen de: CN120400375A
The invention discloses a double-target primer probe composition, application and a kit. The double-target primer probe composition comprises amplification primers and fluorescent probes of a salmonella invasion related gene InvA and an avian pathogenic escherichia coli aerobacin receptor gene IutA. The composition can be used for detecting pathogenic bacteria in poultry farms, and is high in sensitivity, good in specificity, reliable in result and free of cross interference among primers; the kit is suitable for a recombinase polymerase amplification technology, can be used for clinical diagnosis and epidemic condition monitoring of avian pathogenic escherichia coli and salmonella, and has excellent industrial application value.
Resumen de: CN119907682A
Provided herein are vaccine compositions comprising a Salmonella antigen conjugated to a capsid wherein the capsid comprises a wild-type or native sequence. Also provided herein are vaccine compositions comprising a Salmonella antigen conjugated to a capsid wherein the capsid comprises at least one mutation, such as a non-natural mutation. Such compositions are useful in the prevention or treatment of Salmonella infection (salmonellosis), in the treatment and prevention of gastroenteritis, typhoid and/or paratyphoid; and the method can effectively aim at various salmonella strains.
Resumen de: MX2025007790A
Methods and antimicrobial compositions are provided for surface cleaning. The antimicrobial compositions which include very low concentrations of an organic acid and one or more anionic surfactants can, unexpectedly, provide effective and broad spectrum antimicrobial activity within two minutes of contact with a surface and in some instances within one minute of contact time. Contacting the antimicrobial compositions with a surface can result in greater than or equal to about 4.0 log kill or 5.0 log kill for one or more of <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i>, and <i>Salmonella enterica </i>on the surface in two minutes or less. The compositions can have antimicrobial activity against at least one gram-positive bacterium, gram-negative bacterium, virus, and fungus in two minutes or less of contacting the composition with a surface.
Nº publicación: CN120399983A 01/08/2025
Solicitante:
BEIJING ACAD OF AGRICULTURE AND FORESTRY SCIENCES
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Resumen de: CN120399983A
The invention relates to lactococcus lactis capable of producing nisin as well as construction and application of engineering bacteria of the lactococcus lactis. The invention provides a Lactococcus lactis ZLL111 strain, which is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No.31943. The Lactococcus lactis ZLL111 strain has the advantages that the Lactococcus lactis ZLL111 strain is prepared from Lactococcus lactis; the lactococcus lactis provided by the invention has a relatively strong antibacterial effect, produces nisin, has the characteristics of acid resistance, high temperature resistance, protease resistance and the like, and has a relatively strong anti-salmonella effect in vitro. Genetically engineered bacteria are constructed by using the bacteriocin, and the expression quantity is improved.
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