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Device and method for treating aquaculture wastewater by utilizing microalgae

Resumen de: CN120903746A

The invention provides a device and a method for treating aquaculture wastewater by utilizing microalgae, and particularly relates to the technical field of wastewater biological treatment.The device comprises a primary sedimentation tank, a pretreatment tank, an anaerobic tank, an aerobic tank and a microalgae reaction tank which are sequentially connected, a combustion chamber is arranged on the outer side of the pretreatment tank, the pretreatment tank is connected with an ultrasonic cell crusher, and the microalgae reaction tank is connected with an anaerobic tank; the combustion chamber is connected with the anaerobic tank, and the anaerobic tank is also connected with the microalgae reaction tank; the primary sedimentation tank, the pretreatment tank, the anaerobic tank, the aerobic tank and the microalgae reaction tank are arranged in parallel side by side, and a material transfer structure is arranged above the tanks. According to the device and the method for treating the breeding wastewater by utilizing the microalgae, the microalgae can be used for treating livestock and poultry breeding wastewater, efficient treatment of the breeding wastewater is realized, and the device and the method have the technical characteristic of multiple functions.

Shrimp seed breeding method for regulating and controlling water quality through microalgae and probiotics under specific illumination

Resumen de: CN120898753A

The invention discloses a shrimp seed breeding method for regulating and controlling water quality through microalgae and probiotics under specific illumination. According to the technical scheme, the shrimp seed breeding method is characterized by comprising the following steps that firstly, a seedling breeding workshop is built; step 2, microalgae-probiotic culture: selecting microalgae and probiotic types beneficial to shrimp seedling culture, and culturing to obtain a microalgae solution and a probiotic solution; 3, seedling raising and putting preparation; step 4, putting shrimp larvae; step 5, putting of algae-probiotics; step 6, seedling culture illumination control; 7, feeding the young shrimps with bait; 8, monitoring and regulating water quality; and 9, evaluating the young shrimps out of the pond. According to the method, illumination is precisely controlled, the growth environment of microalgae and probiotics is optimized, and the synergistic effect of the microalgae and probiotics in the aspects of water purification, nutrition supply, disease prevention and control and the like is fully exerted, so that the water quality of penaeus vannamei larvae is improved, the survival rate and health condition of the penaeus vannamei larvae are improved, and the survival rate of penaeus vannamei larvae is increased. And a good foundation is laid for large-scale and high-efficiency culture of the penaeus vannamei.

Carbon sequestration system for synergistically purifying industrial aquaculture tail water based on fish-algae symbiosis

Resumen de: CN120903718A

The invention discloses a carbon sequestration system for synergistically purifying industrial aquaculture tail water based on fish-algae symbiosis, which comprises a filter cloth filter tank, an ozone catalytic oxidation system, a microalgae photobioreactor, an algae-water separation device and a filter material filter tank, water quality pretreatment is carried out through an ozone catalytic oxidation system, most of ammonia nitrogen and nitrite nitrogen in water are oxidized into nitrate nitrogen, then the nitrate nitrogen enters a microalgae photobioreactor, algae liquid obtained after reaction passes through an algae-water separation device, filtrate and filter residues are obtained, the filtrate is disinfected and filtered and then returns to the factory-like culture pond, and the water quality is purified. The method comprises the following steps of: recycling the raw materials of the microalgae photobioreactor to achieve the purpose of circulation, drying the filter residue in a factory to prepare a high-protein feed raw material to achieve the purpose of resource reutilization, and meanwhile, purifying and pretreating the tail gas reaching the standard in the factory, and introducing the tail gas rich in carbon dioxide into the microalgae photobioreactor to achieve the purpose of biological carbon sequestration.

EXTRACELLULAR VESICLES FROM MICROALGAE, THEIR BIODISTRIBUTION UPON INTRANASAL ADMINISTRATION, AND USES THEREOF

Resumen de: US2025339483A1

Provided are compositions containing microalgae extracellular vesicles (MEVs) formulated for intranasal delivery, whereby, upon intranasal administration the MEVs traffic through specific routes following intranasal administration to specific regions in the brain via the olfactory nerve and throughout the lateral olfactory tract (LOT) to interconnected brain regions. The MEVs traffic via neuronal axonal transport. The MEVs have the ability to cross-over synapses including: (i) the synapses between the olfactory sensory neurons (OSN) and the mitral/tufted neurons; (ii) the synapses between the mitral/tufted neurons and the local neurons in the various brain regions colonized by the lateral olfactory tract (LOT); and (iii) the synapses between the neurons in the brain regions colonized by the LOT and neurons from the frontal cortex, the hippocampus, the thalamus, and the hypothalamus. The compositions contain extracellular vesicles from microalgae (MEVs) that are loaded with bioactive cargo for treating, detecting, diagnosing, or monitoring a disease, disorder, or condition of the brain or involving the brain, particularly providing neuronal delivery of the cargo. The compositions and methods have a variety of applications as therapeutics and diagnostics for treating, diagnosing, and monitoring a disease, disorder, or condition of the brain or involving the brain. The compositions can be used in methods and uses for treating cancers involving the brain, and can be used, for exa

COMPACT MANNER OF GROWING MICROALGAE SUITABLE FOR CARBON SEQUESTRATION AND CREATING A CIRCULAR ECONOMY

Resumen de: US2025340806A1

There are closed systems of growing microalgae but as they are not able to produce algal biomass economically they are very few and far between and are only used to grow microalgae for very expensive produce. These closed systems also require large space. The result of the lack of economic closed systems is why almost all algal biomass production currently uses raceway ponds. This invention integrates a new compact. very economic and highly productive closed system of growing microalgae including good parameter control with carbon sequestration from say a coal fired power plant or organic waste management in a way that makes the whole process financially attractive and cyclic and if desired it could be made carbon negative. The compact nature of this invention allows algal farms to be sited next to most sources of carbon dioxide.

SUSTAINABLE PHOTOBIOREACTOR

Resumen de: WO2025227221A1

Photobioreactors are described herein. The photobioreactors include a vessel for cultivating microalgae. The vessel defines an interior chamber comprising a culture containing medium. The vessel has at least one transparent wall to permit passage of light of a frequency necessary to promote growth of the microalgae in the culture containing medium. The vessel also includes a plurality of sensors, each sensor being configured to monitor at least one environmental condition within the photobioreactor, and at least one flow generator positioned within the vessel. The photobioreactor also includes a controller communicatively coupled to each of the plurality of sensors and the at least one flow generator. The controller is configured to: receive sensor data from each of the plurality of sensors, apply a mathematical model to the sensor data to determine when to adjust at least one environmental condition within the photobioreactor and adjust one or more components of the photobioreactor.

DEVICE AND METHOD FOR CAPTURING ATMOSPHERIC POLLUTANTS

Resumen de: WO2025227212A1

The invention relates to a structure and method for air purification and addresses a need in the field of sustainability, providing a sustainable alternative for improving air quality with emphasis on the capture of CO2 and carbon monoxide using aquatic organisms, microalgae, and cyanobacteria.

USE OF MICROALGAE AND MICROALGAE DERIVED COMPONENTS IN CULTIVATED FOOD PRODUCTION

Resumen de: AU2023416213A1

Provided herein are methods for cultivated food production involving the use of microalgae. The methods include recycling spent cell media that are used to cultivate cells for cultivated food production as well as enriching culture medium used to cultivate the same cells. The microalgae are used to recycle the spent culture medium. The microalgae may be used to extract microalgae derived components to enrich the culture medium. The algae recycling and algae enriched growth media as provided herein may be used with any animal cell culture. In some variations, the microalgae or microalgae derived components may be used as a raw ingredient in the preparation of cell based seafood products. The methods provided herein may be used for the production of seafood products. The methods herein may provide at least one added advantage—the use of algae may add nutrients, flavors, and aromas typically desired in seafood.

Chlorella sorokiniana M6, application thereof and method for synchronously synthesizing lutein and protein

Resumen de: CN120888408A

The invention belongs to the technical field of microorganisms, and discloses chlorella sorokiniana M6, application of the chlorella sorokiniana M6 and a method for synchronously synthesizing xanthophyll and protein. The preservation number of the Chlorella sorokiniana M6 provided by the invention is GDMCC NO.66205, the Chlorella sorokiniana M6 has an excellent capability of co-producing lutein and protein, and is almost free of chlorophyll synthesis, so that the Chlorella sorokiniana M6 has very important economic value and practical significance for realizing commercial application of microalgae protein and lutein production.

Preparation method of microalgae extract

Resumen de: CN120888410A

The invention belongs to the technical field of preparation of extracts, and particularly relates to a preparation method of a microalgae extract. The preparation method of the microalgae extract comprises the following steps: (1) adding sterile water into microalgae in a logarithmic phase to obtain a microalgae mixed solution; (2) spraying the microalgae mixed solution obtained in the step (1) on the surface of a culture carrier, then adding the culture carrier into a microalgae culture medium added with ammonium niobate for culture, and collecting a product; and (3) adding the product in the step (2) into an ethanol water solution, heating, refluxing and extracting to obtain an extracting solution, and concentrating and drying the extracting solution to obtain the microalgae extract. The culture carrier is used in the microalgae culture process, and the ammonium niobate component is added into the microalgae culture medium, so that the content of protein and polysaccharide in the prepared microalgae extract is obviously increased.

Microalgae lipid extraction method by utilizing triple-effect cell wall degrading enzyme synergy and ultrasonic assistance

Resumen de: CN120884926A

The invention provides a microalgae lipid extraction method using triple-effect cell wall degrading enzyme synergy and ultrasonic assistance, which comprises the following steps: suspending microalgae powder in a buffer solution containing triple-effect cell wall degrading enzyme, and carrying out enzymolysis to obtain a microalgae enzymolysis mixed suspension; the triple-effect cell wall degrading enzyme comprises cellulase, pectinase and mannase; carrying out ultrasonic treatment on the microalgae enzymolysis mixed suspension in an ice bath, centrifuging, discarding supernatant, and collecting precipitated biomass to obtain wall-broken algae powder; the ultrasonic treatment adopts a pulse mode; mixing the wall-broken algae powder with a mixed solvent of n-hexane and isopropanol, extracting, centrifuging to take supernate, and evaporating and concentrating to obtain the lipid extract. A triple-effect cell wall degrading enzyme system is adopted to precisely degrade a polysaccharide network of microalgae cell walls through a synergistic effect; secondly, ultrasonic treatment is carried out under the ice-bath low-temperature condition, the algal alkene hydrophobic barrier on the outer layer of the microalgae cell wall can be efficiently and selectively damaged, and finally the lipid extraction rate is increased.

Application and harvesting method for harvesting wide-salinity microalgae through cationic polymer compounded bentonite flocculation

Resumen de: CN120888409A

The invention discloses application of cationic polymer compounded bentonite flocculation for harvesting wide-salinity microalgae and a harvesting method, and belongs to the technical field of environmental protection. The method comprises the following steps: adding a polydimethyldiallylammonium chloride solution and a sodium bentonite suspension into an algae solution according to the cell biomass of microalgae to be harvested, stirring, settling and collecting to finish harvesting. According to the method disclosed by the invention, the microalgae in the wide-salt environment are harvested by combining PDDAC and BE for flocculation, the microalgae can be efficiently flocculated and harvested within the range of 0-40% of water salinity, and the harvesting rate can reach 90% or above; the flocculating efficiency is high, the dosage of a flocculating agent is small, the floc is large in size and compact, and the salt-resistant characteristic is remarkable; the method is suitable for efficient harvesting of microalgae in artificial culture or natural water under a wide salinity condition, provides a key technology for the field of microalgae harvesting, and has a wide application prospect; the problems of poor salinity tolerance of a flocculating agent, large dosage of the flocculating agent and poor harvesting effect in microalgae harvesting through flocculation at present are solved.

Microbial composition for realizing nitrogen reduction and synergism of flooded rice field as well as preparation method and application of microbial composition

Resumen de: CN120888540A

The invention provides a microbial composition for realizing nitrogen reduction and synergy of a flooded rice field as well as a preparation method and application of the microbial composition, and belongs to the technical field of rice field nitrogen reduction. The microbial composition disclosed by the invention comprises cyanobacteria microspheres and pseudomonas fluorescens microspheres, wherein the mass ratio of the cyanobacteria microspheres to the pseudomonas fluorescens microspheres is (4.8-5.2): (2.8-3.2); the cyanobacteria microspheres and the pseudomonas fluorescens microspheres are independently packaged. The cyanobacteria microspheres are spread on the water surface of a rice field 7-10 days before rice transplanting; in the early tillering stage of the rice, the pseudomonas fluorescens microspheres are applied to a 5-10cm soil layer at the rhizosphere of the rice, so that the nitrogen loss amount of the flooded rice field is reduced, the nitrogen fertilizer utilization rate and the rice yield of the rice are improved, and a technical support is provided for ensuring the stable yield and yield increase of the rice under the condition that the nitrogen fertilizer application amount of the flooded rice field is reduced.

Bioreactor for microalgae culture

Resumen de: CN120888377A

The invention provides a bioreactor for microalgae culture. The bioreactor comprises a base, a culture bin fixedly installed on the base and an inner light supplementing assembly installed in the culture bin. The inner light supplementing assembly comprises a light supplementing pipe arranged in the center of the culture bin, and the light supplementing pipe is transparent and foldable or telescopic; a telescopic screw rod and an adjusting threaded pipe which is in threaded connection with the telescopic screw rod are arranged in the light supplementing pipe; the telescopic screw is fixedly connected to the inner top end of the light supplementing pipe; an adjusting motor used for driving the adjusting threaded pipe to rotate is installed in the base. The base is provided with an incidence channel for an external light source to enter, and the light source passes through the incidence channel, then is conducted into the light supplementing pipe and then is scattered into the culture bin. According to the device, the light supplementing pipe is arranged in the culture bin, and an external light source enters the culture bin after passing through the light supplementing pipe, so that microalgae in the center in the culture bin can obtain more sufficient illumination, the growth and reproduction of the microalgae are more facilitated, and the biomass concentration of the microalgae is improved.

Preparation method of euglena fermentation product filtrate with antioxidant effect

Resumen de: CN120884086A

The invention belongs to the technical field of microalgae biology, and particularly relates to a preparation method of euglena fermentation product filtrate with an anti-oxidation effect. According to the present invention, the patent algae species are fermentation algae species, fermentation and extraction are performed under the special process, and the freezing clarification method and the physical sterilization method are combined, such that the unpleasant odor and the strong color in the euglena fermentation filtrate are effectively removed, and the retention of the active substances in the filtrate is ensured; and an excellent and reliable raw material source is provided for deep utilization and innovative development of subsequent related products. In addition, the euglena fermentation filtrate is non-toxic, harmless and pollution-free, meets the environmental protection standard, contains a large amount of active ingredients, and is a preferred raw material for food and medicine development.

INTENSIVE METHOD OF BIO-COAGULATION FOR THE NON-EXCHANGE OF WATER FROM JUVENILE WATER FROM LABORATORY DULLFISH SPECIES

Resumen de: MX2024004473A

The present invention relates to a laboratory-juvenile intensive production process using a water-sparing biophlocuation system for the reproduction of organisms selected from at least one sweet aquaculture species, namely decapod crustaceans, including acocilles, freshwater lobsters, crayfish, and river crabs, which enables the production of juveniles for experimental and commercial production. The system allows better control of the production by using domesticated players from a selected genetic core to be able to intensify the biomass in a system without replacement of water, allowing the optimisation of the performance by controlling the processes of development of bacteria, yeasts and microalgae, used in bio-coagulation, and also limit the entry of harmful agents.

Composition containing extracellular vesicles derived from microalgae

Resumen de: TW202541824A

The purpose of the present invention is to provide a composition that contains extracellular vesicles derived from microalgae and has suppressed discoloration. In one embodiment of the present invention, a composition containing (A) extracellular vesicles derived from microalgae, (B) a polyhydric alcohol, and (C) at least one chemical selected from the group consisting of organic acids, salts thereof, and nonionic surfactants, is prepared.

Light energy weight self-adaptive microalgae internal and external light source complementary carbon sequestration system

Resumen de: CN120866027A

The invention belongs to the technical field of microalgae carbon sequestration, and particularly relates to a light energy weight self-adaptive microalgae internal and external light source complementary carbon sequestration system, which comprises a light culture assembly made of a transparent material and used as a container for containing algae liquid; the light supplementing assembly is arranged in the light culture assembly; the detection system is used for detecting illumination intensity data, illumination angle data, algae liquid biomass concentration data and light field distribution data in the light culture assembly; the control system is electrically connected with the detection system and the light supplementing assembly, and the control system adjusts the illumination intensity of the light supplementing assembly according to the illumination intensity data, the illumination angle data, the light field distribution data and the algae liquid biomass concentration data. According to the system, the light quality and the light intensity of a light source are accurately regulated and controlled by predicting the light field distribution in the light culture assembly and adjusting the light supplementing intensity of the light supplementing assembly, so that light field reconstruction is realized, stable carbon sequestration of microalgae is assisted, and high-quality target carbon neutralization is realized.

Efficient biological carbon sequestration method and system device for coupling culture of microalgae based on flue gas and by-products after decarbonization by ammonia process

Resumen de: CN120866073A

The invention discloses a high-efficiency biological carbon sequestration method and system device for coupling culture of microalgae based on flue gas and by-products after ammonia decarburization, and the method is characterized in that the flue gas (the temperature is 25-35 DEG C, and the CO2 concentration is 1-5%) after the ammonia decarburization is directly used as a microalgae carbon source, and the by-product ammonium sulfate (the adding concentration in a culture medium is 0.1-2.5 g/L) after the ammonia decarburization is used as a nitrogen source; ammonium bicarbonate (the addition concentration in the culture medium is 0.1-2.5 g/L) is used as a nitrogen source and an auxiliary carbon source, microalgae are inoculated into the prepared culture medium, the flue gas decarbonized by the ammonia process is directly introduced, and photosynthetic culture is carried out. The pH value of a culture medium is monitored in real time, the flow of flue gas obtained after ammonia decarburization and the adding proportion of ammonium bicarbonate and ammonium sulfate are cooperatively fed back and regulated, the pH of a culture system is kept stable, carbon and nitrogen sources are sufficient, high-precision stable control over the culture environment is achieved, and efficient biological carbon sequestration is achieved.

Full-nutrient fresh algae gel for 3D printing and preparation method of full-nutrient fresh algae gel

Resumen de: CN120859151A

The invention provides a preparation method of full-nutrient fresh algae gel for 3D printing, which comprises the following steps: mixing and dissolving starch, a rheological property changing agent, nutritive salt required by microalgae growth and boiling water, adding microalgae cells, and uniformly stirring to form bio-ink for 3D printing. The full-nutrition fresh algae gel not only can support efficient culture of microalgae, but also can be directly used as a full-nutrition fresh algae gel functional food after being subjected to microalgae culture due to the full-food-grade raw materials of the full-nutrition fresh algae gel.

MCP transgenic expression vector and application thereof

Resumen de: CN120866369A

The invention belongs to microalgae bioengineering, and particularly relates to an MCP transgenic expression vector and application thereof. The invention provides an MCP recombinant plasmid, the MCP recombinant plasmid is transferred into microalgae for the first time, the microalgae is not only an initial feed but also can be used as an expression vector of an exogenous gene, and the MCP gene of DIV1 is used for preparing a microalgae oral vaccine, so that the vacancy of a medicine for preventing and treating the full-eye iridovirus 1 at present is made up, the homology of medicine and food is realized, and the aim of preventing and treating the full-eye iridovirus 1 is fulfilled. The invention provides an environment-friendly, safe and effective transgenic microalgae vaccine drug, promotes the development of an aquatic vaccine technology, and has far-reaching significance on the stable and sustainable development of global aquaculture industry.

Microalgae culture method

Resumen de: CN120866071A

The invention relates to the technical field of microalgae culture, and discloses a microalgae culture method. The invention relates to a microalgae culture method, which comprises the following steps: (1) in the presence of a fat degradation inhibitor, culturing microalgae species to obtain a microalgae solution; (2) separating, adsorbing and degerming the algae solution to obtain a culture solution; and (3) returning the culture solution to the step (1) for reuse. The method can effectively control the generation of organic matters with a growth inhibition effect in the microalgae culture process, improve the production rate and reduce the culture water consumption.

Bacterial agent for producing methane by fermenting blue-green algae bottom mud

Resumen de: CN120866149A

The invention discloses a cyanobacteria bottom mud fermentation methanogenic bacterial agent, which comprises clostridium butyricum, bacillus amyloliquefaciens and methanosarcina martensii, and is characterized in that the clostridium butyricum accounts for 30-60% of the total number of bacterial cells, the bacillus amyloliquefaciens accounts for 25-50% of the total number of the bacterial cells, and the methanosarcina martensii accounts for 30-60% of the total number of the bacterial cells. The methanosarcina marzeberi accounts for 10%-25% of the total number of bacterial cells. The advanced technology of adding microbial compound bacteria and combining high-temperature and high-pressure hydrolysis and anaerobic digestion technologies is utilized, the biodegradability is improved by destroying the cell structure of organic matter, the efficiency and stability of traditional anaerobic fermentation are remarkably improved, the VS removal rate after pyrohydrolysis fermentation reaches 67.5%-70.4%, the gas production rate in the anaerobic fermentation process after pyrohydrolysis is effectively increased, and the production cost is reduced. The yield of methane can be increased, the water body environment can be improved, recycling of bottom mud resources can be promoted, and green and sustainable ecological restoration is achieved.

Beta-carotene hydroxylase mutant, preparation method and application

Resumen de: CN120866251A

The invention discloses a beta-carotene hydroxylase mutant as well as a preparation method and application thereof, and belongs to the technical field of microalgae gene engineering. Amino acid Cys No. 191 of haematococcus pluvialis beta-carotene hydroxylase is mutated into Ala, amino acid Thr No. 213 of haematococcus pluvialis beta-carotene hydroxylase is mutated into Ala, and the yield of astaxanthin is increased by 1.282 times and 1.268 times respectively. According to the mutant obtained through mutation, the catalytic efficiency and substrate selectivity of beta-carotene hydroxylase are remarkably improved, astaxanthin is produced more specifically through enzymatic reaction, and a key mutation direction is provided for construction of engineering strains for high yield of astaxanthin.

In-situ online observation device applied to microalgae growth dynamic response process

Nº publicación: CN120870119A 31/10/2025

Solicitante:

CHONGQING UNIV
\u91CD\u5E86\u5927\u5B66

Resumen de: CN120870119A

The invention provides an in-situ online observation device applied to a microalgae growth dynamic response process. The in-situ online observation device comprises an in-situ online observation device and an automatic shooting system, the in-situ online observation device comprises a culture observation plate, a mainstream micro-channel and a micro-culture chamber; the culture observation plate is transparent; the mainstream micro-channel is arranged on the upper side of the culture observation plate; a flow inlet and a flow outlet are respectively formed in two ends of the main flow micro-channel in the length direction; the plurality of micro-culture chambers are communicated with the main flow micro-channel; the plurality of micro-culture chambers are arranged between the flow inlet and the flow outlet; the automatic shooting system is used for shooting microalgae in the micro-culture chamber; the in-situ online observation device is simple and effective in structure and convenient to manufacture, and has the characteristics that internal nutrients and metabolic oxygen are efficiently transported and transferred, and the nutrients are continuously, stably and uniformly distributed; and in combination with an automatic shooting system, long-time continuous imaging observation can be carried out on the intrinsic dynamic response process of the growth of a plurality of microalgae cells, and theoretical guidance is provided for the maximum growth potential of the microalgae c