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System and process for coupling carbon capture in air and microalgae electric energy

NºPublicación:  CN120536209A 26/08/2025
Solicitante: 
TIANJIN UNIV
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CN_120536209_PA

Resumen de: CN120536209A

The invention discloses a system and a process for coupling carbon capture in air and microalgae electric energy. The system comprises an air carbon capture unit, a microalgae culture unit, a harvesting dehydration unit, and an electric energy management unit and a resource circulation unit which are connected with the units, wherein the air carbon capture unit comprises a three-stage dust removal subsystem, a modular temperature swing adsorption tower group and an intelligent transportation subsystem; the microalgae culture unit comprises a multi-layer composite vertical raceway pond, a spectrum-adjustable LED illumination system and a multi-parameter cooperative control system; the harvesting and dewatering unit comprises an inclined plate precipitation-air flotation combined pre-concentration device, a centrifugation-electric field coupling separation system and a double-cone rotary low-temperature vacuum dryer. Through the synergistic effect of the MOFs adsorbent, the multi-layer vertical raceway pond and multi-energy complementary intelligent scheduling, the core targets of low energy consumption, low cost and high carbon sequestration efficiency are achieved, and a reproducible and extensible circular economy mode is further constructed.

DIABETIC SKIN DISEASE MEDICAL COMPOSITION CONTAINING EXOSOMES DERIVED FROM MICROALGAE

NºPublicación:  KR20250127240A 26/08/2025
Solicitante: 
주식회사위튼컴퍼니

Resumen de: KR20250127240A

메디컬 조성물은 미세 조류를 광활성 배양하는 것, 광활성 배양된 미세 조류를 초음파 분쇄하여 세포 용해물을 생성하는 것, 및 세포 용해물로부터 엑소좀을 추출하는 것을 포함하는 엑소좀 추출 방법에 의해 획득된 엑소좀을 포함한다.

Oyster biological reaction system construction method, colored oyster feeding method and oyster product

NºPublicación:  CN120530912A 26/08/2025
Solicitante: 
YANTAI INSTITUTE OF COASTAL ZONE RES CHINESE ACADEMY OF SCIENCES
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CN_120530912_PA

Resumen de: CN120530912A

The invention discloses an oyster biological reaction system construction method, a colored oyster feeding method and an oyster product. The oyster biological reaction system construction method comprises the following steps: screening out an optimal filter feeding environment condition of oysters; screening the microalgae filtering efficiency of the oysters under the optimal filtering environment condition, and determining the optimal filtering concentration of the oysters to the microalgae; the oysters are placed under the optimal filter feeding environment condition, and different types of microalgae are selected to be densely fed for a short time at the optimal filter feeding concentration; the method comprises the following steps: carrying out stage division on interception, absorption and conversion conditions of microalgae in oysters from a time scale by taking content change of symbolic components in the oysters and nutritional components as indexes, and constructing oyster biological reaction systems in different stages. By means of the method, the oysters can be fed within a short time, rapid nutrition conversion is achieved, the pigment level of edible parts of the oysters is improved, the quality of the oysters is improved, and meanwhile the accurate nutrition enrichment type colored oysters can be created.

MICROALGAE PRODUCTION METHOD AND MICROALGAE PRODUCTION APPARATUS

NºPublicación:  JP2025123709A 25/08/2025
Solicitante: 
MITSUBISHI CHEM CORP
UNIV CHUO
\u4E09\u83F1\u30B1\u30DF\u30AB\u30EB\u682A\u5F0F\u4F1A\u793E
JP_2025123709_A

Resumen de: JP2025123709A

To provide a microalgae production method and a microalgae production apparatus that are capable of reliably releasing dissolved oxygen, which increases in concentration in the culture solution, from the culture solution.SOLUTION: In one embodiment, the method employs: supplying a gas containing carbon dioxide to a culture solution containing microalgae; circulating the culture solution between a buffer tank that stores the culture solution and a sealed cultivation unit for cultivating the microalgae, by using a circulation pump; measuring the dissolved oxygen concentration of the culture solution while circulating the culture solution between the buffer tank and the sealed cultivation unit; measuring the pressure of the culture solution in the piping through which the culture solution flows while circulating the culture solution between the buffer tank and the sealed cultivation unit; and controlling the pressure in the piping of the sealed cultivation unit so that dissolved oxygen is evaporated from the culture solution in the sealed cultivation unit.SELECTED DRAWING: None

Methods for manufacturing nutritional enhancement of live feed using a nutrient-rich Microalgae Schizochytrium sp. Biomass

NºPublicación:  KR20250126197A 25/08/2025
Solicitante: 
씨제이제일제당주
WO_2025174210_PA

Resumen de: WO2025174210A1

The present invention relates to: a feed composition for zooplankton, comprising biomass derived from Schizochytrium sp. microalgae; a method for culturing zooplankton using same; and an aquaculture method for fish or crustaceans. The biomass derived from Schizochytrium sp. microalgae of the present invention has the effect of nutritionally enriching zooplankton, and when zooplankton fed with the biomass is fed to fish or crustaceans, growth of fish or crustaceans is promoted and non-specific immunity is enhanced, and thus the biomass can be effectively used as a feed composition or a feed additive.

METHOD FOR CULTURING MICROALGAE AND METHOD FOR PRODUCING LIPID

NºPublicación:  JP2025123675A 25/08/2025
Solicitante: 
UNIV KOBE
MITSUBISHI CHEM CORP
\u56FD\u7ACB\u5927\u5B66\u6CD5\u4EBA\u795E\u6238\u5927\u5B66
JP_2025123675_PA

Resumen de: JP2025123675A

To provide a technique for lipid production employing algae.SOLUTION: The present invention provides microalgae, wherein the microalgae show lipid production capability under nitrogen-sufficient conditions and, in cultivation under conditions maintaining a nitrogen concentration of 17 mg/L or more in the medium, the microalgae can exhibit a lipid content of 20% or more based on dry algal body weight.SELECTED DRAWING: Figure 3

MICROALGAE PRODUCTION METHOD AND MICROALGAE PRODUCTION APPARATUS

NºPublicación:  JP2025123942A 25/08/2025
Solicitante: 
MITSUBISHI CHEM CORP
UNIV CHUO
\u4E09\u83F1\u30B1\u30DF\u30AB\u30EB\u682A\u5F0F\u4F1A\u793E
JP_2025123942_A

Resumen de: JP2025123942A

To provide microalgae production method and microalgae production apparatus that are capable of maintaining the total inorganic carbon concentration necessary for microalgae growth and reducing pH fluctuations in the microalgae culture solution.SOLUTION: In one embodiment, the method employs: supplying a gas containing carbon dioxide to a culture solution containing microalgae; circulating the culture solution between a buffer tank that stores the culture solution and a cultivation unit for cultivating the microalgae using a circulation pump; measuring the pH of the culture solution while circulating the culture solution between the buffer tank and the cultivation unit; and, when the pH of the culture solution is 7 or higher, controlling the supply amount of the gas containing carbon dioxide based on the pH of the culture solution.SELECTED DRAWING: None

Slow sinking fish feed and preparation method thereof

NºPublicación:  CN120514065A 22/08/2025
Solicitante: 
FOSHAN SHUNDE HAIHUANG IND CO LTD
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CN_120514065_A

Resumen de: CN120514065A

The present invention discloses a slow sinking fish feed and a preparation method thereof, and relates to the technical field of fish feeds, the slow sinking fish feed comprises basic raw materials: 200 kg of mulberry leaves, 138 kg of soybean meal 43%, 150 kg of peanut bran, 60 kg of fermented soybean meal, 60 kg of rapeseed meal, 100 kg of rice enzymolysis protein, and 150 kg of corn; additives: 20 kg of monocalcium phosphate, 40 kg of refined deep sea fish oil, 50 kg of microalgae meal, 2 kg of multi-vitamin for fish, 5 kg of organic multi-mineral for fish, 1 kg of VC powder, 2 kg of choline, 5 kg of table salt and 2 kg of liver and gallbladder protecting elements; the functional core comprises a composite functional system comprising 3kg of repair group microspheres and 2kg of support group particles, and 10kg of mulberry extract, the contradiction between the response speed and the mechanical strength of a temperature-sensitive material can be overcome, the problem of low self-repair efficiency in a water environment is solved, and the problems of feed pulverization caused by temperature difference, high loss of an antioxidant in water and high cost are solved.

Accurate temperature gradient regulation and control method and device

NºPublicación:  CN120519640A 22/08/2025
Solicitante: 
QINGDAO HENGNUO SHIJIA SEAWEED TECH CO LTD
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CN_120519640_PA

Resumen de: CN120519640A

The invention discloses a precise temperature gradient regulation and control method and device, and belongs to the field of microalgae cultivation. A precise temperature gradient regulation and control device comprises a transparent cultivation tank, a stirring shaft is rotationally connected into the cultivation tank, a plurality of sets of stirring paddles are installed on the stirring shaft at equal intervals, at least one temperature sensor is arranged on the stirring paddles on the same plane, and a driving part for driving the stirring shaft to rotate is arranged on the cultivation tank; the stirring shaft rotates at a low speed to stir microalgae in the culture tank to move, so that the microalgae are kept in a suspended state and receive uniform illumination, and the culture effect of the microalgae is improved; meanwhile, through cooperation of a reciprocating lead screw, a magnetic suction plate and a piston plate, air can be continuously inflated into cultivation liquid in the cultivation tank, and through cooperation of stirring and the upward floating effect of airflow, the microalgae can make uniform contact with carbon dioxide in the air, the uniform respiration effect is achieved, and the cultivation effect of the microalgae is further improved.

System and optimization process for coupling chemical absorption of DAC and photosynthetic fixation of microalgae

NºPublicación:  CN120519260A 22/08/2025
Solicitante: 
TIANJIN UNIV
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CN_120519260_PA

Resumen de: CN120519260A

The invention discloses a system for coupling DAC chemical absorption and microalgae photosynthetic fixation and an optimization process. The device comprises a microalgae culture unit, a harvesting and dehydrating unit and a control module, the microalgae culture unit is a photobioreactor, an LED light source capable of adjusting light intensity, an aeration distribution pipeline and a propeller stirring system are arranged in the photobioreactor, the photobioreactor is filled with a 0.5 mM sodium carbonate solution which is used for absorbing CO2 in the atmosphere, and the concentration of the CO2 in the microalgae culture unit is 0.5 mM; the harvesting and dehydrating unit sequentially comprises a centrifugal separation device, a plate-and-frame filter pressing device and a heat pump drying device or a solar auxiliary drying device, and is used for concentrating and drying algae liquid. Through innovative coupling of DAC and microalgae photosynthetic immobilization and combination of culture medium, illumination and gas mass transfer system optimization, a complete technical route integrating CO2 capture, carbon sequestration, biomass output and environment evaluation is formed, and remarkable environmental and economic values are achieved.

Recombinant escherichia coli for producing astaxanthin and method for synthesizing astaxanthin

NºPublicación:  CN120519359A 22/08/2025
Solicitante: 
INST OF HYDROBIOLOGY CHINESE ACADEMY OF SCIENCES
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CN_120519359_PA

Resumen de: CN120519359A

The invention relates to recombinant Escherichia coli for producing astaxanthin, which is obtained by taking an Escherichia coli BW-ASTA strain as a chassis strain and transferring a cyanobacteria deacylase gene CddA into the chassis strain, the nucleotide sequence of the cyanobacteria deacylase gene CddA is as shown in SEQ ID NO. 1. After the CddA is over-expressed in the engineered escherichia coli, the synthesis efficiency of the astaxanthin in the escherichia coli is improved, a feasible path is provided for green production and industrial production of the astaxanthin, and the method has a commercial transformation prospect. The invention also relates to a synthesis method of astaxanthin, which comprises the following steps: culturing the recombinant escherichia coli to obtain astaxanthin, dynamically monitoring growth parameters (cell density and metabolic state) of the escherichia coli, and accurately controlling IPTG induction opportunity to coordinate thallus growth and resource allocation of astaxanthin anabolic flow, thereby realizing the balance of high yield and low cost.

Microalgae crust decontaminating agent for radioactive surface contamination and use method of microalgae crust decontaminating agent

NºPublicación:  CN120519229A 22/08/2025
Solicitante: 
SOUTHWEST UNIV OF SCIENCE AND TECHNOLOGY
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CN_120519229_PA

Resumen de: CN120519229A

The invention discloses a microalgae crust detergent for radioactive surface contamination, which comprises the following components in percentage by mass: 2.5 to 3 percent of polyvinyl alcohol, 1 to 2 percent of sodium carboxymethyl cellulose, 1 to 1.5 percent of plasticizer, 0.2 to 1 percent of microalgae frond, 0.5 to 1 percent of dispersing agent and 90 to 95.5 percent of water. All the components are mixed and stirred to obtain the microalgae crust detergent for radioactive surface pollution. The prepared microalgae crust detergent not only can effectively remove radioactive substances from various surfaces, but also is good in film-forming property, easy to strip, free of damage to treated objects and suitable for various radioactive contamination scenes; in addition, the microalgae crust detergent disclosed by the invention also has good biodegradability and meets the environmental protection requirement.

Selenized microalgae preparation and application thereof in preparation of immune preparation for treating tumors

NºPublicación:  CN120514865A 22/08/2025
Solicitante: 
SOOCHOW UNIV
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CN_120514865_PA

Resumen de: CN120514865A

The invention relates to a selenized microalgae preparation and application thereof in preparation of immune preparations for treating tumors. The microalgae are induced and cultured in a selenium-rich solution for several days, and then are collected and injected to the tumor site to inhibit the growth of the tumor site. The preparation process of the selenized microalgae is simple, and the selenized microalgae can stimulate an organism to generate strong immune response, activate dendritic cells and T cells and regulate an immunosuppressive microenvironment at a tumor through various pathways such as NF-kappa B, so that an efficient and long-term tumor suppression effect is realized; meanwhile, the microalgae can enhance immune checkpoint blocking treatment, and a certain foundation is laid for combined application of microorganism-mediated immunotherapy and immune checkpoint blocking.

Purification treatment method for tail water of aquatic product

NºPublicación:  CN120518226A 22/08/2025
Solicitante: 
HUNAN AOJUN TECH CO LTD
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CN_120518226_PA

Resumen de: CN120518226A

The invention provides a purification treatment method for tail water of aquatic products, and belongs to the technical field of sewage treatment. Adding the symbiotic aggregates or the modified symbiotic aggregates into the tail water of the aquatic product, stirring, detecting the indexes of COD and NH4 < + >-N in the treated water, and discharging after the indexes reach the standard; the symbiotic granules are prepared from chlorella, pseudomonas and cladosporium herbaceous through mixed symbiosis; the modified symbiotic aggregate is prepared by mixing chlorella, pseudomonas, cladosporium herbacea and immobilized enzyme hollow CNT-CuS/TiO2 microspheres for symbiosis and embedding the mixture in sodium alginate. The preparation method comprises the following steps: mixing chlorella, pseudomonas, cladosporium herbacea and immobilized enzyme hollow CNT-CuS/TiO2 microspheres for symbiosis. According to the invention, sewage treatment is carried out through a facultative bacteria + fungus + microalgae symbiotic aggregate structure, and the advantages of good purification treatment effect, fast treatment speed, good purification effect, low cost, no need of additional addition of a carbon source, reduction of the oxygen introduction amount of a sewage treatment system, simplification of the sewage treatment system, reduction of energy consumption, reduction of cost and the like are achieved.

Carbon absorption device for microalgae carbon sequestration

NºPublicación:  CN223255205U 22/08/2025
Solicitante: 
ANHUI ZHONGKE MICROALGAE BIOTECHNOLOGY CO LTD
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CN_223255205_U

Resumen de: CN223255205U

The utility model discloses a carbon absorption device capable of being used for microalgae carbon sequestration, and belongs to the technical field of microalgae carbon sequestration. The utility model discloses a carbon absorption device for microalgae carbon sequestration. The carbon absorption device comprises a first reactor and a second reactor, wherein the lower ends of the first reactor and the second reactor are respectively provided with a gas inlet pipeline and a liquid discharge pipe, and the upper ends of the first reactor and the second reactor are respectively provided with a gas outlet; the spraying device is arranged at the upper end of the second reactor; the first liquid inlet is formed in the upper end of the first reactor; the aeration disc is arranged in the first reactor; according to the utility model, the reactors are connected in series, the first reactor adopts a bubbling mode for reaction, the whole process is simple and quick, the cost is lower, the second reactor adopts a spraying mode for secondary reaction, the utilization rate of carbon dioxide is higher, and the final reaction effect is greatly improved through the double-reactor double-mode reasonable series design; and resource waste and production cost are reduced.

METHODS FOR MANUFACTURING NUTRITIONAL ENHANCEMENT PRODUCT FOR LIVE FEED USING NUTRIENT-RICH MICROALGAE SCHIZOCHYTRIUM SP. BIOMASS

NºPublicación:  WO2025174210A1 21/08/2025
Solicitante: 
CJ CHEILJEDANG CORP [KR]
\uC528\uC81C\uC774\uC81C\uC77C\uC81C\uB2F9 (\uC8FC)
WO_2025174210_PA

Resumen de: WO2025174210A1

The present invention relates to: a feed composition for zooplankton, comprising biomass derived from Schizochytrium sp. microalgae; a method for culturing zooplankton using same; and an aquaculture method for fish or crustaceans. The biomass derived from Schizochytrium sp. microalgae of the present invention has the effect of nutritionally enriching zooplankton, and when zooplankton fed with the biomass is fed to fish or crustaceans, growth of fish or crustaceans is promoted and non-specific immunity is enhanced, and thus the biomass can be effectively used as a feed composition or a feed additive.

APPARATUS AND METHOD TO REMOVE TRITIUM FROM HIGH VOLUMETRIC WASTEWATERS

NºPublicación:  US2025266181A1 21/08/2025
Solicitante: 
KOREA ATOMIC ENERGY RES INSTITUTE [KR]
KOREA ATOMIC ENERGY RESEARCH INSTITUTE
US_2025266181_PA

Resumen de: US2025266181A1

The present disclosure relates to an apparatus and method for treating high volumetric wastewater, containing tritium, and more particularly to an apparatus for treating wastewater, containing tritium, includes a container storing wastewater, containing tritium, an LED cable including an LED light source, a gas supply cable, and a transparent cover seating the container, wherein the gas supply cable is provided to supply air and carbon dioxide in a form of fine bubbles, and a method for treating wastewater, containing tritium, includes an operation of pouring wastewater, containing tritium, into a container of the apparatus for treating wastewater, containing tritium; an operation of injecting microalgae into the container; and an operation of inducing photosynthesis by using light of an LED cable under fine bubbles supplied by a gas supply cable.

ENGINEERED MICROORGANISM FOR PRODUCTION OF CANNABINOIDS

NºPublicación:  JP2025121918A 20/08/2025
Solicitante: 
ALGAE C INC
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JP_2025121918_PA

Resumen de: JP2025121918A

To provide a genetically engineered microorganism capable of producing a cannabinoid.SOLUTION: Provided is a genetically engineered microorganism that is a photosynthetic microalgae or cyanobacterium and does not contain an exogenous nucleic acid molecule encoding an aromatic prenyltransferase. In one aspect, the genetically engineered microorganism is capable of producing tetrahydrocannabinolic acid or tetrahydrocannabinol and does not contain an exogenous nucleic acid molecule encoding a tetrahydrocannabinolic acid synthase.SELECTED DRAWING: Figure 1

Rural sewage microalgae treatment device

NºPublicación:  CN223239906U 19/08/2025
Solicitante: 
CHANGZHOU INSTITUTE OF INDUSTRY TECH
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CN_223239906_U

Resumen de: CN223239906U

The utility model discloses a rural sewage microalgae treatment device, which belongs to the technical field of sewage treatment, and comprises an algae box and a peristaltic pump, the top of the algae box is provided with a first illumination component, one side of the algae box is provided with a plurality of circulation ports, the other side of the algae box is provided with a discharge port, the circulation ports are fixedly connected with a circulation component, and the discharge port is provided with a discharge port. A bracket is arranged at the bottom of the circulating assembly, a second illumination assembly is arranged at the top of the bracket, and a mixing assembly is arranged in the circulating assembly. The first illumination assembly and the second illumination assembly are arranged, sewage in the algae tank is irradiated by the LED lamp post, the mixing assembly is continuously irradiated by the LED lamp strip, sufficient illumination on algae is achieved, under the combined action of the circulating assembly, the mixing assembly and the peristaltic pump, the mixing time of the sewage can be prolonged through the mixing pipeline, and the mixing efficiency is improved. Under the guiding action of the spiral blade, the water flow rotates, and the algae and the sewage are mixed by the rotating water flow, so that the effects of fully mixing the sewage and improving the mixing efficiency are realized.

Anti-inflammatory compositions comprising microalgae-derived useful substances

NºPublicación:  KR20250123496A 18/08/2025
Solicitante: 
선문대학교산학협력단
KR_20250123496_PA

Resumen de: KR20250123496A

본 발명은 미세조류 유래 유용물질을 포함하는 항염증 조성물에 관한 것으로, 구체적으로 미세조류 추출물을 포함하는 항염증 조성물, 상기 조성물을 포함하는 건강기능식품에 관한 것이다.

A composition for improving preventing and treating of bone disease comprising microalgae extract

NºPublicación:  KR20250123503A 18/08/2025
Solicitante: 
선문대학교산학협력단
KR_20250123503_PA

Resumen de: KR20250123503A

본 발명은 골질환의 개선, 예방 또는 치료용 조성물에 관한 것으로, 보다 상세하게는 미세조류 추출물을 유효성분으로 함유함으로써, 골질환을 개선, 예방 또는 치료할 수 있으므로, 식품 조성물, 나아가 건강기능식품 또는 약학 조성물로 활용될 수 있다.

Method for the generation and extraction of metabolites from microalgae

NºPublicación:  TW202532636A 16/08/2025
Solicitante: 
TAIAM PAN ALGASIS INC [TW]
TAIAM PAN-ALGASIS, INC
TW_202532636_A

Resumen de: TW202532636A

This invention provides a method for the generation and extraction of metabolites from microalgae. By subjecting microalgal cultures to stress treatments, the microalgae are stimulated to increase the secretion of metabolites. Additionally, this invention utilizes hydrolytic enzymes or fungal cultures to break down the cell walls of the microalgae, thereby releasing the microalgal metabolites more effectively. Finally, methods such as filtration, centrifugation, and ultrafiltration are employed to separate and purify the released metabolites from the microalgae. This technique enhances the yield of microalgal metabolites and is of significant value for applications in biotechnology, food, and pharmaceutical industries involving microalgae.

High-salinity wastewater treatment method and device for realizing granulation of bacteria and algae and synergistic denitrification

NºPublicación:  CN120483392A 15/08/2025
Solicitante: 
HEBEI UNIV OF TECHNOLOGY
TIANJIN RESEARCH INSTITUTE FOR WATER TRANSP ENGINEERING M O T
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\u4EA4\u901A\u8FD0\u8F93\u90E8\u5929\u6D25\u6C34\u8FD0\u5DE5\u7A0B\u79D1\u5B66\u7814\u7A76\u6240
CN_120483392_PA

Resumen de: CN120483392A

The invention relates to the technical field of wastewater treatment, and discloses a high-salinity wastewater treatment method and device for achieving bacterium-alga granulation and synergistic denitrification, the high-salinity wastewater treatment method for achieving bacterium-alga granulation and synergistic denitrification comprises the following steps: placing microalgae and aerobic granular sludge in a photobioreactor for initial culture; after initial culture, adding magnetic iron oxide nanoparticles into the photobioreactor, and carrying out enhanced culture, so as to obtain the algal-bacterial symbiotic aerobic granular sludge, and carrying out denitrification treatment on wastewater by utilizing the bacteria-algae symbiotic aerobic granular sludge. The magnetic iron oxide nanoparticles are added in the growth process of bacteria and algae, so that the problems of slow aggregation and nucleation of bacteria and algae, low activity of photosynthetic electron carriers of algae cells under high salt stress, reduction of denitrification efficiency and the like in the ABGS formation process can be effectively solved; rapid aggregation and nucleation of bacteria-algae cells can be synchronously enhanced, the electron transfer efficiency of an algae cell photosynthetic system under high salt stress is improved, and the denitrification efficiency of microorganisms is improved.

Method for constructing acid-resistant microalgae based on ferredoxin

NºPublicación:  CN120485238A 15/08/2025
Solicitante: 
ZHEJIANG UNIV
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CN_120485238_PA

Resumen de: CN120485238A

The invention discloses an acid-resistant microalgae construction method based on ferredoxin, and belongs to the field of biology and molecular biology. The method specifically comprises the following steps: taking Phatr3J33543 with a sequence structure as shown in SEQ ID No.1, Phatr3J50516 with a sequence structure as shown in SEQ ID No.2 or Phatr3Jdraft1806 with a sequence structure as shown in SEQ ID No.3 as a target gene, removing a termination codon of the target gene, and connecting a fluorescent protein gene with an initiation codon removed through a DNA (Deoxyribose Nucleic Acid) sequence for coding glycine to obtain a recombinant gene; inserting the recombinant gene into multiple cloning sites of a pPhaNR plasmid to obtain a vector plasmid; introducing the carrier plasmids into diatom cells through electroporation, and inoculating the diatom cells to an ESAW solid selective culture medium containing bleomycin to grow brown algal colonies; selecting the algal colonies with fluorescent protein signals from the algal colonies, namely the acid-resistant engineering algae. Compared with wild algae, the acid resistance of the engineering algae obtained by the invention is obviously improved. The method has important reference significance on acid-resistant diatom cultivation.

Acid-resistant microalgae construction method based on bicarbonate ion transporter protein

Nº publicación: CN120485237A 15/08/2025

Solicitante:

ZHEJIANG UNIV
\u6D59\u6C5F\u5927\u5B66

CN_120485237_PA

Resumen de: CN120485237A

The invention discloses an acid-resistant microalgae construction method based on bicarbonate ion transporter protein, and belongs to the field of biology and molecular biology. The method specifically comprises the following steps: taking Phatr3J33543 with a sequence structure as shown in SEQ ID No.1, Phatr3J50516 with a sequence structure as shown in SEQ ID No.2 or Phatr3Jdraft1806 with a sequence structure as shown in SEQ ID No.3 as a target gene, removing a termination codon of the target gene, and connecting a fluorescent protein gene with an initiation codon removed through a DNA (Deoxyribose Nucleic Acid) sequence for coding glycine to obtain a recombinant gene; inserting the recombinant gene into multiple cloning sites of a pPhaNR plasmid to obtain a vector plasmid; introducing the carrier plasmids into diatom cells through electroporation, and inoculating the diatom cells to an ESAW solid selective culture medium containing bleomycin to grow brown algal colonies; selecting the algal colonies with fluorescent protein signals from the algal colonies, namely the acid-resistant engineering algae. Compared with wild algae, the acid resistance of the engineering algae obtained by the invention is obviously improved. The method has important reference significance on acid-resistant diatom cultivation.

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