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Monoclonal antibody for detecting surface protein SapA of campylobacter fetus and application of monoclonal antibody

Resumen de: CN122404548A

The invention discloses a monoclonal antibody for detecting surface protein SapA of campylobacter fetus and application of the monoclonal antibody. The monoclonal antibodies are named as 4F6 and 7B3 respectively, and amino acid sequences of variable regions of heavy chains and light chains of the two antibodies are disclosed. The monoclonal antibody prepared by the invention can specifically recognize the surface protein SapA of the campylobacter fetus, has better specificity, and does not have cross reaction with non-campylobacter such as standard escherichia coli, salmonella, listeria, klebsiella pneumoniae, staphylococcus aureus, streptococcus and the like; no cross reaction with campylobacter jejuni, campylobacter colon and other campylobacter is generated; the test strip prepared by combining the monoclonal antibody and colloidal gold can realize on-site rapid detection of the campylobacter fetus, the detection limit is low, the sensitivity is high, and an immunological method capable of specifically detecting the campylobacter fetus is successfully developed.

Primer for detecting Pseudomonas tolaasi and application of primer

Resumen de: CN122405862A

The invention provides a primer for detecting Pseudomonas tolaasi, the primer comprises a forward primer and a reverse primer, the nucleotide sequence of the forward primer is as shown in SEQ ID No: 1, and the nucleotide sequence of the reverse primer is as shown in SEQ ID No: 2. The method for detecting the Pseudomonas tolaasi comprises the following steps: extracting genome DNA (Deoxyribonucleic Acid) of a sample to be detected, taking the DNA as a template, carrying out PCR (Polymerase Chain Reaction) amplification by utilizing the forward primer and the reverse primer, carrying out agarose gel electrophoresis detection on a PCR amplification product, and judging whether the sample to be detected contains the Pseudomonas tolaasi or not. The invention also provides an application of the primer for detecting the Pseudomonas tolaasi, and the primer is used for preparing a kit for detecting the Pseudomonas tolaasi, and the application of the primer for detecting the Pseudomonas tolaasi is used for detecting the Pseudomonas tolaasi. The primer for detecting Pseudomonas tolaasi, which is designed by the invention, has the advantages of strong specificity, no need of isolated culture and biochemical identification, short detection period, high efficiency, good universality and wide application prospect in allusion to a specific gene of Pseudomonas tolaasi.

Method, composition and kit for rapidly detecting two food-borne pathogenic pseudomonas

Resumen de: CN122405858A

The invention relates to an oligonucleotide primer probe composition for detecting food-borne pathogenic pseudomonas bacteria (pseudomonas alcaligenes and pseudomonas buchneri) based on an ERA fluorescence method or an ERA test strip method, and a detection method using the composition. The pseudomonas alcaligenes or pseudomonas buchneri in the sample can be rapidly, specifically and sensitively detected, and the method is easy and convenient to operate, short in reaction time and free of complex temperature control equipment, and can be widely applied to rapid screening, pollution monitoring and risk early warning of food-borne pathogenic pseudomonas in various food samples such as milk and dairy products.

Multiple Raman/visual dual-mode detection method and kit for pathogenic bacteria based on Cas12a cis/trans-cleavage activity

Resumen de: CN122385571A

The invention discloses a single-tube multiple Raman/visual detection method and kit for pathogenic bacteria based on Cas12a and an in-situ crRNA synthesis strategy, and belongs to the technical field of food safety detection. By designing single-molecule allosteric hairpin DNA containing a target recognition region, a T7 promoter and a crRNA template, 16S rRNA of specific response target bacteria, hairpin structure recombination and crRNA in-situ synthesis are started, and cis-and trans-cleavage of Cas12a is guided, so that rapid visual detection of the total bacteria amount and SERS identification and quantitative detection of different pathogenic bacteria targets are realized. The method solves the problem of insufficient qualitative accuracy and quantitative sensitivity caused by the interference of a biological matrix in the existing rapid detection method of the pathogenic bacteria, overcomes the dependence of a CRISPR/Cas12a method on a target PAM site, and greatly widens the detection range of the nucleic acid target of the food-borne pathogenic bacteria. The Raman/visual dual-mode detection system adapts to a field rapid detection scene, the detection limit is as low as the single cell level, the specificity and reproducibility are excellent, and the Raman/visual dual-mode detection system can be used for scenes such as food safety control and live pathogenic bacterium diagnosis.

Salmonella detection system based on RPA-CRISPRCas12a and application thereof

Resumen de: CN122382226A

The invention discloses a salmonella detection system based on RPA-CRISPRCas12a and application of the salmonella detection system. The detection system comprises an RPA (recombinase polymerase amplification) primer pair, a probe, crRNA (ribonucleic acid) and LbCas12a protein, the sequences of the RPA amplification primer pair are as shown in SEQ ID NO. 1 and SEQ ID NO. 2; the sequence of the probe is as shown in SEQ ID NO.7; and the sequence of the crRNA is as shown in SEQ ID NO. 8. The RPA-CRISPR/Cas12a visual detection system has the advantages of high sensitivity, strong specificity, simplicity and convenience in operation, rapidness in detection and the like, the lowest detection limit of salmonella reaches 1 fg/mu L, and stable and repeatable fluorescence signals and visual results can still be generated under the concentration. The system effectively overcomes the defects of a traditional detection method, is suitable for on-site rapid screening and risk early warning of salmonella in a food chain, provides technical support for food safety supervision, and also provides a reference technical scheme for rapid detection of other food-borne pathogenic bacteria.

CRISPR-Cas12a-based rapid detection method for food-borne pathogenic bacteria

Resumen de: CN122382221A

The invention relates to the technical field of nucleic acid detection, in particular to a food-borne pathogenic bacterium rapid detection method based on CRISPR-Cas12a. According to the method, after genome DNA is extracted from a food sample, bridging primers of an upstream primer combination identification gene segment and a downstream primer combination virulence gene segment are provided, the two segments are located on the same continuous DNA molecule at the interval of 50-1500 bp, and the two segments are located on the same continuous DNA molecule; amplifying for 20-40 minutes at the constant temperature of 37 DEG C, and amplifying to obtain a chimeric amplicon only when the two segments are linked to the same molecule; and activating Cas12a trans-cleavage activity by combining crRNA of a guide sequence spanning a junction site with a chimeric amplicon, cleaving a single-stranded DNA reporter probe and detecting a signal. In the single-tube constant-temperature reaction, ligase is not needed, the linkage state of the gene and the virulence gene is directly confirmed and identified, the detection limit is 10-100 CFU/mL, and the time consumed in the whole process is less than 60 minutes.

Composite probe and test strip for detecting food-borne pathogenic bacteria and application of composite probe and test strip

Resumen de: CN122385882A

The invention provides a composite probe and a test strip for detecting food-borne pathogenic bacteria and application of the composite probe and the test strip, and belongs to the technical field of food-borne pathogenic bacteria detection. The composite probe comprises a bacteriophage functional capture probe and a ZMPAP signal composite probe; the bacteriophage functional capture probe contains a specific inactivated bacteriophage aiming at the food-borne pathogenic bacteria; the ZMPAP signal composite probe is prepared from a ZIF-67 metal organic framework material, methylene blue, polydopamine, gold nanoparticles and porcine immune globulin G. Compared with a single signal detection technology, the method disclosed by the invention has visual qualitative and accurate quantitative capabilities, is strong in specificity and good in repeatability, and provides a core technical support for on-site rapid prevention and control of the food-borne pathogenic bacteria.

Method for rapidly detecting streptococcus suis or/and salmonella based on RAA-EXO technology and application thereof

Resumen de: CN122382222A

The invention discloses a method for rapidly detecting streptococcus suis or/and salmonella based on an RAA-EXO technology and application of the method. According to the method, specific RAA primers and EXO probes are designed and screened aiming at streptococcus suis recN genes and salmonella invA genes, the sequences of the specific RAA primers and the EXO probes are shown as SEQ ID NO.3, 5 and 10 and SEQ ID NO.14, 15 and 20 respectively, a single or double RAA-EXO detection system for the streptococcus suis and the salmonella is established, detection can be completed within 20 minutes under the condition of the constant temperature of 39-41 DEG C, and the detection sensitivity is high. And no cross reaction with common bacteria such as escherichia coli, staphylococcus aureus and the like exists. Results can be visually judged through a fluorescence curve or a blue light instrument, an effective tool is provided for rapid, sensitive and on-site detection of streptococcus suis and salmonella, and the kit has important application value in the fields of food safety and epidemic disease prevention and control.

Super-multiplex PCR (polymerase chain reaction) primer combination capable of simultaneously detecting 35 pathogenic bacteria and fecal indicator bacteria and application of super-multiplex PCR primer combination

Resumen de: CN122382225A

The invention discloses a super-multiplex PCR (polymerase chain reaction) primer combination capable of simultaneously detecting 35 pathogenic bacteria and fecal indicator bacteria and application of the super-multiplex PCR primer combination. The super-multiplex PCR primer combination comprises 35 pairs of target specific primers, and nucleotide sequences of the 35 pairs of target specific primers are shown as SEQ ID NO.1-70. The method for detecting 35 pathogenic bacteria and fecal indicator bacteria comprises the following steps: extracting DNA of a sample to be detected to obtain a DNA mixture of a target sample; carrying out ultra-multiplex PCR amplification on the target sample DNA mixture to obtain a target amplification product, and carrying out high-throughput sequencing to obtain a target amplification product sequencing result; and detecting and judging the to-be-detected sample. According to the method, generation of dimers among the primers and nonspecific amplification are effectively reduced, homogenized amplification of 35 targets in a single reaction system is realized, and the method can be applied to the fields of food-borne pathogenic bacterium detection, public health emergency detection and the like.

O45 Escherichia coli detection antibody and application thereof

Resumen de: CN122356274A

The invention relates to the field of biological medicine, in particular to an O45 Escherichia coli detection antibody and an application method thereof. The antibody is a monoclonal antibody or an antigen binding fragment thereof, and comprises a heavy chain CDR sequence as shown in SEQ ID NO: 1-3 and a light chain CDR sequence as shown in SEQ ID NO: 4-6. The invention also provides a nucleic acid molecule for coding the antibody, an expression vector, a host cell and a preparation method thereof. Specific identification results show that the antibody is specifically combined with O45 Escherichia coli, has no cross reaction with O157, O26, O103, O111, O121, O145 and other Escherichia coli serotypes and O55 related serotypes, and also has no cross reaction with common food-borne pathogenic bacteria. The detection limit of an indirect ELISA detection method established based on the antibody to O45 Escherichia coli can reach 1 * 10 < 2 > CFU/mL, and the whole detection process can be completed within 24 hours. The monoclonal antibody provided by the invention can be applied to preparation of detection kits, detection test paper and other products, and is suitable for rapid screening of O45 Escherichia coli in import and export food.

DETECTION OF ATYPICAL PNEUMONIA

Resumen de: EP4772522A2

Disclosed herein are methods and compositions for detecting one or more pathogens that cause atypical pneumonia. Detectable pathogens include Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila.

Fluorescent rapid detection reagent and application thereof

Resumen de: CN122344612A

The invention provides a fluorescent rapid detection reagent and application thereof, and belongs to the technical field of bacterial detection. The rapid fluorescent detection reagent provided by the invention comprises a bacterium specific aptamer, an initiation chain and hairpin structures H1, H2 and H3. By establishing a fluorescent specific amplification detection system with strong specificity and high sensitivity, a fluorescent signal amplification marker aiming at staphylococcus aureus and pseudomonas aeruginosa susceptible to rodents and a handheld visual detection technology of the fluorescent signal amplification marker are developed, and the fluorescent signal amplification marker and the handheld visual detection technology are prepared into a detection reagent, so that rapid and sensitive detection of target bacteria is realized, and the detection efficiency is improved. The method has important significance on screening detection and quality control of pathogenic microorganisms of experimental animals.

Food-borne pathogen single-tube multiple detection sequence combination based on asymmetric ratio and melting curve, kit, detection method and application

Nº publicación: CN122326781A 03/07/2026

Solicitante:

SICHUAN ZHENXING TESTING TECH CO LTD
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Resumen de: CN122326781A

The invention relates to the field of gene detection, in particular to a food-borne pathogenic bacterium single-tube multiple detection sequence combination based on an asymmetric ratio and melting curve, a kit, a detection method and application. The invention provides a sequence combination for detecting food-borne pathogenic bacteria. The sequence combination comprises a forward primer, a reverse primer, a specific probe and a reverse partial complementary melting curve probe. The invention further provides a kit for detecting the food-borne pathogenic bacteria, the problems of system competitive inhibition and cross mismatch under a complex genetic background are solved by adjusting an asymmetric ratio, and a detection method for detecting type 5 diarrheagenic Escherichia coli, salmonella, listeria monocytogenes and staphylococcus aureus through a single tube is constructed. The method does not need to add a complex hardware channel or expensive detection equipment, has the advantages of high detection flux, strong specificity and low instrument cost, and is suitable for application and popularization of low-resource grass-roots institutions, customs and first-line food safety supervision.